scholarly journals Antimicrobial activity of herbal extracts against Vibrio spp. bacteria isolated from white feces syndrome on white leg shrimp (Litopenaeus vannamei) in some provinces in the mekong delta

2021 ◽  
Vol 13 (2) ◽  
pp. 61-68
Author(s):  
Minh Ut Truong ◽  
Thi Tu Uyen Dao ◽  
Thanh Dung Tu
Keyword(s):  
Antibacterial Activity ◽  
Mekong Delta ◽  
Abundant Species ◽  
Psidium Guajava ◽  
Herbal Extract ◽  
Diffusion Method ◽  
Herbal Extracts ◽  
Opportunistic Pathogens ◽  
Phyllanthus Urinaria ◽  
Vibrio Spp

The study was conducted to determine the antibacterial activity of three herbal extracts: guava (Psidium guajava), leafflower (Phyllanthus urinaria L), beach daisy (Wedelia biflora (L.) DC ) against Vibrio spp. isolated from white feces syndrome infected shrimp in some provinces in the Mekong Delta. The antibacterial activity of the three herbal extracts was evaluated by the well diffusion method, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The results indicated that there were 102 isolates in total of 378 shrimp samples which were clarified into 7 groups. Among these group, Vibrio alginolyticus group were the most abundant species with the percentage of 50 %, followed by Vibrio cholerae group with the percentage of 17.7%. The herbal extracts of P. guajava and P. urinaria L showed a broad - spectrum antibacterial activity against ten isolates which were selected for MIC and MBC test. The extract of P. urinaria L had the strongest antibacterial activity against V. harveyi CM3HPA2 and V. alginolyticus CM3IB2 (MIC of 0.2 mg/ml, MBC of 0.39 mg/ml). The obtained results indicated that the herbal extract of P. urinaria L will be a good candidate for reducing opportunistic pathogens Vibrio spp. abundant in gastrointestinal tract of shrimp.

scholarly journals IN VITRO ANTAGONISTIC ACTIVITIES OF INDONESIAN MARINE SPONGE AAPTOS AAPTOS AND CALLYSPONGIA PSEUDORETICULATA EXTRACTS AND THEIR TOXICITY AGAINST Vibrio spp.

2011 ◽  
Vol 6 (2) ◽  
pp. 173 ◽  
Author(s):  
Rosmiati Rosmiati ◽  
Habsah Mohamad ◽  
Tengku Sifzizul Tengku Muhammad ◽  
Najiah Musa ◽  
Aziz Ahmad ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Diffusion Method ◽  
Larval Rearing ◽  
Butanol Extract ◽  
Vibrio Spp ◽  
Mic Value

Vibriosis is one of diseases which often results in mass mortality of Penaeus monodon larval rearing systems. It attacks shrimp of all stages in zoea, mysis and shrimp postlarva stage. This disease is caused by Vibrio spp, particularly Vibrio harveyi (a luminescent bacterium). Several kinds of antibiotics and chemical material have been used to overcome the disease but they have side effects to environment and human. The searching of bioactive compounds as an alternative treatment has been done for multi purposes. In this study diethyl eter, butanol and aqueous extract of Indonesian sponges Aaptos aaptos and Callyspongia pseudoreticulata were tested for in vitro activity against Vibrio spp. and Vibrio harveyi by using disc diffusion method. The result showed that all extracts of Aaptos aaptos gave a positive antibacterial activity towards those pathogenic bacteria. Meanwhile, only butanol extract of Callyspongia pseudoreticulata obtained to exhibit an antibacterial activity on those pathogenic bacteria. The strong anti-vibrio activity were shown by butanol and aqueous extract of Aaptos aaptos with the minimum inhibitory concentration (MIC) value of 0.313 and 0.625 mg/mL, respectively. Whilst, the butanol extract of Callyspongia pseudoreticulata indicated a low antibacterial activity with the MIC value of 10 mg/mL. Toxicity of those active extracts was evaluated by Brine Shrimp Lethality Test (BST). Interestingly, butanol and aqueous extracts of Aaptos aaptos did not show any toxic effect in Artemia salina larvae up to 8 x MIC (2.504 mg/mL and 5.000 mg/mL). It is the first report for the anti-vibr io activity of both Aaptos aaptos and Callyspongia pseudoreticulata. This results suggest that Aaptos aaptos has a potential to be used as a source of alternative compound to vibriosis prevention for mariculture.

scholarly journals ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT OF WHITE AND RED FLESH FROM GUAVA LEAF ( Psidium guajava. L) AGAINTS Staphylococcus aureus AND Escherichia coli

2016 ◽  
Vol 16 (1) ◽  
pp. 51-56
Author(s):  
Hilda Maysarah ◽  
Rika Apriani ◽  
Misrahanum Misrahanum
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Activity Test ◽  
Agar Diffusion Method ◽  
Guava Leaf ◽  
Mic Value

An antibacterial activity test of ethanol extract of white and red flesh from guava leaf (Psidium guajava. L) against S.aureus and E.coli; using agar diffusion method was carried out in order to produce the extract. The extract was collected using maceration method. The concentration of extract was 7,8125; 6,1035; 5,00; 4,8828; 4,3944; and 3,90625 mg/mL. The results showed that both of extracts had antibacterial activities. Ethanol extract of white flesh of fruit guava leaf had (Minimum Inhibitory Concentration) MIC value at 5.000 mg/mL against S.aureus and 4.8828 mg/mL against E.coli. Whereas ethanol extract of red flesh of fruit guava leaf had MIC value at 4.3944 mg/mL against S.aureus and E.coli.  MIC value of ethanol extract of white flesh of fruit guava leaf is equal with MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli. The MIC value of red flesh of fruit guava leaf is equal to the MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli.

scholarly journals Antibacterial Activity of Locally Prepared Herbal Cough Extracts against Klebsiella pneumoniae and Streptococcus pneumoniae

2019 ◽  
pp. 1-9
Author(s):  
Atuheirwe Maxine ◽  
Jacob Stanley Iramiot
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pneumoniae ◽  
Klebsiella Pneumoniae ◽  
Diffusion Method ◽  
Dilution Method ◽  
Herbal Extracts ◽  
Bacterial Strains ◽  
Zones Of Inhibition ◽  
Tract Infections

Aim: Cough due to Klebsiella pneumoniae and Streptococcus pneumoniae is currently managed by conventional antibiotics and herbal extracts in Uganda. However, much as these herbal extracts are extensively used, their antibacterial activity is not known. This study aimed at determining the antibacterial activity of the selected locally prepared herbal cough extracts against two bacterial strains i.e. Klebsiella pneumoniae (ATCC 700603), and Streptococcus pneumoniae (ATCC 49619). Methods: The herbal cough extracts were screened for antibacterial activity using Agar-well diffusion method for determining zone of inhibition, macro broth dilution method for Minimum Inhibitory Concentration (MIC) determination and Streak plate method for Minimum Bactericidal Concentration (MBC). Results: In vitro evaluation of antibacterial activity of the 5 brands of herbal cough extracts against K. pneumoniae and S. pneumoniae   revealed that all extracts possessed significant antimicrobial effects against all microorganisms tested (p < 0.05). However, MM04 (35.6±0.0) mm and MM03 (33.6±1.5) mm had maximum zones of inhibition as compared to other herbal extracts against K. pneumoniae and S. pneumoniae respectively. Average MIC results for extracts against K. pneumoniae   indicated that MM01 had the highest MIC (2.5000 mg/ml) while MM03 had the least MIC (0.0625 mg/ml). Average MIC results for extracts against S. pneumoniae showed MM01 had the highest MIC (2.0000 mg/ml) while MM03 3 had the least MIC (0.0438 mg/ml). Average MBC results for extracts against K. pneumoniae indicated that MM01 had the highest MBC (4.000 mg/ml) while MM03 had the least MBC (0.030 mg/ml). Average MBC results for extracts against S. pneumoniae showed MM01 had the highest MBC (4.000 mg/ml) while MM03 had the least MBC (0.033 mg/ml). Conclusion: The results obtained in present study were revealed that locally prepared herbal extracts had significant antibacterial activity. Hence they can be used as promising alternatives of antibiotics used against Respiratory Tract Infections due to K. pneumoniae and S. pneumoniae.

scholarly journals Assessment in Vitro of Antibacterial Activity of Manipulated Product, on Solution Form, Obtained from Dry Extract of Leaves of Psidium guajava L.

2015 ◽  
Vol 8 (1) ◽  
pp. 64
Author(s):  
Marcos V.S. Silva ◽  
Tayara S. Frazão ◽  
Melyssa M.A.V. Ferreira ◽  
Jandir S. Sales ◽  
Denis B. Vieira ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Cytotoxic Activity ◽  
Staphylococcus Epidermidis ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Dry Extract ◽  
Agar Diffusion ◽  
Agar Diffusion Method

<p class="Default">The <em>Psidium guajava L</em>. specie is a perennial shrub, belonging to the <em>Myrtaceae</em> family and it is popularly known as guava, its leaves are used in therapy for treating various diseases. The study aims to evaluate the antibacterial activity <em>in vitro </em>of manipulated product obtained from dried extract of the leaves of <em>P. guajava </em>L. front standard bacteria ATCC and clinical isolates. The tests were conducted on bacterial samples: <em>Staphylococcus aureus </em>(ATCC 25923), <em>Pseudomonas aeruginosa </em>(ATCC 27883), <em>Escherichia coli </em>(ATCC 25922), <em>Salmonella spp</em>, <em>Acinetobacter baumannii</em>, <em>Proteus mirabilis</em>, <em>Shigella flexneri</em>, <em>Staphylococcus epidermidis</em>, <em>Staphylococcus haemolyticus</em>, <em>Streptococcus agalactiae, Streptococcus mutans</em>. Among the tests can be mentioned phytochemical of the ethanol extract (EE), microbiological control and physical-chemical analysis of the product and microbiological tests such as agar diffusion method (wells), minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), an evaluation test of hemolytic capacity of the solution and in vitro assay cytotoxic activity were performed, . The best result of the product in the agar diffusion method was front <em>Staphylococcus epidermidis</em>, while the lower MIC and MBC were front <em>Staphylococcus aureus </em>(ATCC 25923). The product showed no hemolytic activity and no cytotoxic activity at the tested concentrations. According to the test results, it is believed on the possibility of the production of a pharmaceutical formulation derived from the dry extract of <em>Psidium guajava</em>, since it showed great antibacterial activity.</p>

Assessment of the antibacterial activity of chestnut (Castanea sativa) and cloves (Syzygium aromaticum) herbal extracts as an alternative to antibiotics use during post-hatching period of chicks

2021 ◽  
Vol 11 (4) ◽  
pp. 48-54
Author(s):  
Rafał Kędzia ◽  
Marcin Lis
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Bacterial Infections ◽  
Castanea Sativa ◽  
Herbal Extract ◽  
Control Group ◽  
Syzygium Aromaticum ◽  
Herbal Extracts

Bacterial infections of newly hatched chicks are the most common cause of their death in the initial period of rearing. These infections are always treated with antibiotics. The aim of the study was to investigate the antimicrobial activity of herbal extracts of chestnut (Castanea sativa) and clove (Syzygium aromaticum) against bacterial infections i.e. Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumonice in comparison to antibiotics. The results of the microbiological analyses showed that the Castanea sativa and Syzygium aromaticum extracts had a slighter antibacterial activity in comparison to antibiotics. The diameter of zone inhibition of the culture's growth of gram-negative bacteria (i.e. Escherichia coli and Klebsiella pneumoniae) and gram-positive bacteria (i.e. Staphylococcus aureus and Enterococcus faecalis) was 6-13 mm for these extracts in comparison to 15-30 mm for antibiotics. However, some bacterial strains presented full resistance to the selected antibiotics, e.g., wild strains of Enterobacteriaceae to amoxicillin or Staphylococcus aureus and Enterococcus faecalis to florfenicol, colistin, and doxycycline. In the second experiment, the effect of the herbal extract mixture added into drinking water on the growth and mortality of chicken broiler during the first rearing week was investigated. There was found that the use of herbal extracts improved the chickens’ body weight (157.4 g; P≤0.008) and decreased mortality rate (2.4%) compared to the control group (144.1 g and 3.9%, respectively) but not to the group treated with antibiotic (161.5 and 0.6% respectively; P≤0.009). In summary, the use of herbal extracts as a nutritional supplement for poultry seems to have a positive effect on weight gain of young birds, and to some extent reduce mortality in the first week of rearing.

scholarly journals Study on the antibacterial activity of selected natural herbs and their application in water treatment

2013 ◽  
Vol 6 (2) ◽  
pp. 199-231
Author(s):  
P. S. Harikumar ◽  
C. M. Manjusha
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Herbal Extract ◽  
World Health ◽  
Complete Analysis ◽  
Diffusion Method ◽  
Most Probable Number ◽  
Ocimum Sanctum ◽  
Probable Number ◽  
Ocimum Tenuiflorum

<p><strong>Abstract.</strong> The microbial contamination of water is a world-wide environmental problem. Many traditional methods are being used in various parts of the world to purify the water. According to World Health Organization, 80% of world's population living in rural areas relies on herbal traditional medicines as their primary health care. So the study on properties and uses of medicinal plants are subject to growing interests. An attempt was made to assess the antibacterial properties of certain selected herbs such as <i>Ocimum sanctum</i> (Krishna Tulsi), <i>Ocimum kilimandscharicum</i> (Karpoora Tulsi), <i>Ocimum tenuiflorum</i> (Ram Tulsi), <i>Azadirachta indica</i> (Neem), <i>Simarouba glauca</i> (bitterwood), <i>Caesalpinia sappan</i> (Pathimugam), <i>Cuminum cyminum</i> (Jeerakam), <i>Vetiveria zizanioides</i> (Ramacham), <i>Saraca indica</i> (Ashoka tree) and <i>Murraya koenigii</i> (curry leaves) against different bacteria such as total coliforms, faecal coliforms, <i>Escherichia coli</i>, <i>Bacillus</i> sp. and <i>Serratia</i> sp. The antibacterial activity of the plant extracts was determined by spread plate method, Kirby–Bauer disc diffusion method, most probable number (MPN) method and Petrifilm method. The shelf life of the herbal extract <i>Ocimum sanctum</i> (Krishna Tulsi) was also determined using a UV-visible spectrophotometer. A comparison study of the antibacterial efficiency of the three varieties of <i>Ocimum sanctum, Ocimum kilimandscharicum</i> and <i>Ocimum tenuiflorum</i> was also done. After the complete analysis of the antibacterial activity of different herbs, <i>Ocimum sanctum</i>, the most efficient herb, was selected and treatment methods based on the herb were developed so that it can be used conveniently in various households. Therefore <i>Ocimum sanctum</i> plant can be further subjected to isolation of therapeutic antimicrobial and pharmacological evaluation.</p>

scholarly journals Determination of Antibacterial Activity of Psidium guajava Leaf Extract against Bacteria Isolated from Mobile Phones of Umaru Musa Yar’adua University, Katsina Community

2021 ◽  
Vol 6 (1) ◽  
pp. 219-226
Author(s):  
B. Abdulkadir ◽  
◽  
A. Ado ◽  
A. I. Alli
Keyword(s):  
Antibacterial Activity ◽  
Mobile Phones ◽  
Leaf Extract ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Bacterial Contaminants ◽  
Suggested Keywords ◽  
Zones Of Inhibition ◽  
Aqueous Leaf Extract ◽  
The University

Mobile phones are increasingly becoming one of the indispensable accessories of professional and social daily life, although constant handling and usage in various environmental conditions makes it fomite. Thus, antibacterial activity of Psidium guajava leaf extract was evaluated against bacteria isolated from mobile phones within Umar Musa Yar’adua University, Katsina community. A total of sixty (60) mobile phones were randomly swabbed, twenty (20) each, from hawkers, students, and staff of the University community. These were used as candidates for isolation of bacterial contaminants using standard protocols. Variable concentrations (500mg/ml, 300mg/ml and 100mg/ml) of aqueous extract of the Psidium guajava were prepared and tested against mobile phones bacterial isolates using agar well diffusion method. Preliminary phytochemical screening revealed the presence of flavonoids, cardiac glycosides, saponins, tannins and terpenoids. Staphylococcus aureus (39.6%), Escherichia coli (29.7%), Klebsiella sp. (18.8%), Proteus sp. (8.3%) and Pseudomonas aeruginosa (4.2%) were the bacterial contaminants isolated and identified from the mobile phones. Aqueous leaf extract of the plant displayed promising antibacterial activity at 500mg/ml against all the isolates, with average zones of inhibition of 25.0 mm for S. aureus, Proteus sp., P. aeroginosa and 24mm, 6.0mm for E. coli and Klebsiella sp. respectively. The use of Psidium guajava leaf extract as candidate for production of antibacterial agent which can be used to disinfect mobile handsets is suggested. Keywords: Mobile phones, contaminants, Antibacterial activity, Psidium guajava, antibacterial hand wash

scholarly journals In vitro assessment of the antibacterial activity of combinations of methanolic extracts of Mangifera indica L. bark and Psidium guajava L. leaf on multidrug-resistant Pseudomonas aeruginosa

2021 ◽  
Vol 18 (2) ◽  
pp. 87-94
Author(s):  
Churchill Akena ◽  
Gloria Maxensia Akampa ◽  
Edson Ireeta Munanura ◽  
Robert BD Otto ◽  
Bruhan Kaggwa ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Mangifera Indica ◽  
Psidium Guajava ◽  
Multidrug Resistant ◽  
Positive Control ◽  
Diffusion Method ◽  
Methanolic Extracts ◽  
The Individual

Pseudomonas aeruginosa causes various infections in humans both in hospital and community settings. Mangifera indica Linnaeus bark and dry Psidium guajava Linnaeus leaves have individually demonstrated activity against P. aeruginosa. This study aimed to assess the combined antibacterial activity of methanolic extracts of dry M. indica bark and dry P. guajava leaves on Multidrug-Resistant P. aeruginosa. Different proportion combinations of P. guajava and M. indica were assessed for antipseudomonal activity using Agar well diffusion method. Colistin was the positive control. The Fractional Inhibitory Concentration Index (FICI) was also determined. The combination of methanolic extracts of P. guajava leaves (35 mg/mL) and M. indica bark (6.25 mg/mL) had a superior antibacterial effect on Multidrug-Resistant P. aeruginosa when compared with the individual extracts used alone (p˂0.05), save for P. guajava (100 mg/mL) (p = 0.1373). Colistin was significantly more active on MDR P. aeruginosa than all the test extract concentrations used. This combination of M. indica bark and P. guajava leaves methanolic extracts had a FICI of 0.2434. This study demonstrates that the combination of P. guajava leaves (35 mg/mL) and M. indica bark (6.25 mg/mL) has synergistically enhanced activity against MDR P. aeruginosa. Keywords: Antipseudomonal, MDR Pseudomonas aeruginosa, Mangifera indica, and Psidium guajava

Antibacterial Properties of Chinese Herbal Medicines against Nosocomial Antibiotic Resistant Strains of Pseudomonas aeruginosa in Taiwan

2007 ◽  
Vol 35 (06) ◽  
pp. 1047-1060 ◽  
Author(s):  
Ching-Shen Liu ◽  
Thau-Ming Cham ◽  
Cheng-Hong Yang ◽  
Hsueh-Wei Chang ◽  
Chia-Hong Chen ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Herbal Medicines ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Herbal Extracts ◽  
Chinese Herbal Medicines ◽  
Antibiotic Resistant ◽  
Chinese Herbal ◽  
Resistant Strains

Pseudomonas aeruginosa is well-recognized as a nosocomial pathogen, which exhibits inherent drug resistance. In this study, the antibacterial activity of ethanol extracts of 58 Chinese herbal medicines used in Taiwan were tested against 89 nosocomial antibiotic resistant strains of Pseudomonas aeruginosa. The results gathered by the disc diffusion method showed that 26 out of the 58 herbal extracts exhibited antibacterial activity. Among the 26 herbal extracts, 10 extracts showed broad-spectrum antibacterial activities and were selected for further antibacterial property assay. The minimum inhibitory concentrations (MIC) of the active partition fractions ranged from 0.25 to 11.0 mg/L. The presence of flavonoid compounds in the active fractions of test herbal extracts was observed by the TLC-bioautography. The results from the time-kill assay revealed that most of the herbal extracts completely killed the test organisms within 4 hours. Exposure of the test strains to a sub-MIC level of the herbal extracts for 10 consecutive subcultures did not induce resistance to the active components. A combination of the active herbal fractions with antibiotics showed that one of the herbal medicines, the hexane fraction of Ramulus Cinnamomi, possessed a synergistic effect with tetracycline, gentamycin, and streptomycin. In conclusion, the tested Chinese medical herbs have the potential to be developed into natural antibiotics. This is the first evaluation for screening large amounts of medical plants against nosocomial antibiotic resistant bacteria in Taiwan.

In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

2016 ◽  
Vol 6 (4) ◽  
pp. 68
Author(s):  
Sushma Vashisht ◽  
Manish Pal Singh ◽  
Viney Chawla
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Positive ◽  
Gram Negative ◽  
Shorea Robusta ◽  
Dose Dependent ◽  
Resin Extract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

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