scholarly journals CYTOTOXICITY OF Phaleria macrocarpa (Scheff.) Boerl. FRUIT MEAT AND SEED ETHANOL EXTRACT TO MONONUCLEAR PERIFER NORMAL CELL OF HUMAN BODY

2010 ◽  
Vol 6 (2) ◽  
pp. 212-218
Author(s):  
Endang Astuti ◽  
Deni Pranowo ◽  
Santi Dwi Puspitasari
Keyword(s):  
Cytotoxic Activity ◽  
Anticancer Drugs ◽  
Human Body ◽  
Human Blood ◽  
Normal Cell ◽  
Ethanol Extract ◽  
F Test ◽  
Activity Test ◽  
Phaleria Macrocarpa

There were many research on Phaleria macrocarpa (Scheff.) Boerl. fruit for its activity as possible anticancer. However, there wasn't investigation that P. macrocarpa seed and fruit meat ethanol extract effect to normal cell. The research was conducted to identify the ethanol extract of P. macrocarpa for cytotoxic activity against mononuclear perifer normal cell of human blood. The research comprised several sections including P. macrocarpa seed and fruit meat maceration with ethanol, and the cytotoxic activity test against mononuclear normal cell. The results showed that the ethanol extract of P. macrocarpa fruit meat and seed was slightly toxic against mononuclear normal cell with the LC50 of 3817.54 μg/mL and 1349.29 μg/mL respectively. Tamoxifen and 5-fluorourasil, anticancer drugs were extremely toxic against mononuclear normal cell giving LC50 of 3.52 μg/mL and 4.05 μg/mL. The ANOVA f-test (P

Identification of Secondary Metabolite Compounds and Cytotoxic Activity Test of Papuan Grape (Sararanga Sinuosa Hemsley) Extract using Brine Shrimp Lethality Test (BSLT) Method

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Elsye Gunawan ◽  
Enrick Kharo Etmond ◽  
Linus Yhani Chrystomo
Keyword(s):  
Cytotoxic Activity ◽  
Secondary Metabolite ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Organ System ◽  
Activity Test ◽  
Anti Cancer ◽  
Extraction Stage ◽  
Cancer Agent ◽  
Concentration Series

Papua has a diversity of flora species, one of which is the Papuan Grape (Sararanga sinuosa Hemsley). It is commonly used by the Depapre community, Jayapura, as a stamina booster. This research aims to identify the secondary metabolite compounds, to test the cytotoxic activity of Papuan Grape (Sararanga sinuosa Hemsley) extract, and to determine the best concentration that inhabits the growth of Artemia salina larvae using the BSLT method. This study was conducted with the extraction stage using the maceration method by making use of 96% ethanol solvent. Subsequently, the concentration series 0, 50, 100, 150, 200, 250, 300 ppm of Papuan Grape (Sararanga sinuosa Hemsley) extract were made to test the cytotoxic activity on the mortality of Artemia salina shrimp larvae. The results showed that Alkaloids, Flavonoids, Saponins, and Tannins were compounded as secondary metabolite. An antioxidant research that had been carried out previously had LC50 of green-white fruit (12,49 ± 0,35 mg/ml), orange-red fruit (17,62 ± 3,49 mg/ml) and red fruit (12,23 ± 0,46 mg/ml). The community process one stalk of it into juice and used or consumed it two times a day. An inappropriate dose of traditional medicine usage can affect the organ system and had adverse effects in the future The result of cytotoxic research obtained the value of LC50 in ethanol extract of Papuan Grape was 140,863 ppm, and concentration of 250 ppm was the best concentration to inhibit the growth of shrimp larvae (Artemia salina L). The conclusion of this study was the ethanol extract of Papuan Grape (Sararanga sinuosa Hemsley) showed the highest cytotoxic activity and potentially become an anti-cancer agent.

Uji Aktivitas Sitotoksik Ekstrak Etanol dari Variasi Teh Daun Sirsak (Annona muricata Linn) Terhadap Larva Udang (Artemia salina Leach)

2016 ◽  
Vol 6 (1) ◽  
pp. 13
Author(s):  
Mega Yulia ◽  
Devahimer Harsep Rosi
Keyword(s):  
Cytotoxic Activity ◽  
Green Tea ◽  
Ethanol Extract ◽  
Black Tea ◽  
Artemia Salina ◽  
Tea Leaves ◽  
Lc50 Value ◽  
Oolong Tea ◽  
Activity Test ◽  
Processing Techniques

A research on the cytotoxic activity of several variants leaves of the soursop (Anona muricata Linn). Soursop leaf is processed into three kinds of tea leaves are green tea, black tea, oolong tea. Cytotoxic activity test was conducted using "Brine Shrimp Lethality Test". LC50 value of the three extracts of the leaves of the soursop is 102.32 ppm for ethanol extract of green tea, black tea 38.01 ppm and 63.09 ppm for oolong tea. Soursop leaf tea processing techniques can affect the cytotoxic activity.

scholarly journals Antiproliferation activity of God's crown fruit (Phaleria macrocarpa) extract and fractions against MCM-B2 breast cancer cells

2020 ◽  
Author(s):  
Ratna Puspita ◽  
Hasim ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Ethyl Acetate ◽  
Cancer Cells ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Water Fraction ◽  
Activity Test ◽  
Phaleria Macrocarpa

Breast cancer is a disease caused by abnormal cell proliferation in the breast. God’s crown fruit (Phaleria macrocarpa) and its seed have potential as an antiproliferation of cancer cells. It contains active compounds such as flavonoids, alkaloids, polyphenols, and tannins. The sample of God’s crown fruit was obtained by extraction and fractionation using the maceration method. Cytotoxicity of extracts and fractions was determined using the Brine Shrimp Lethality Test method. The antiproliferation activity test of God’s crown fruit against MCM-B2 was performed using the hemacytometer method. The God’s crown fruit sample consists of crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction. Lethal concentration 50 (LC50) values in crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction were 13.72, 147.55, 405.81, and 149.55 ppm, respectively. The concentration of the test sample was directly used for the antiproliferation activity test on MCM-B2 cells. God’s crown fruit can act as an antiproliferation of MCM-B2. The smallest concentration of those samples has inhibited MCM-B2 cell proliferation which is 3.5 ppm crude ethanol extract lower than 100 ppm doxorubicin. The maximum percentage of the antiproliferation activity of crude ethanol extract (56 ppm) was able to inhibit MCM-B2 cell proliferation by 58.28% while doxorubicin (100 ppm) by 31.2%. This is due to the fact that crude ethanol extract has a lot of complex polar phytochemical content. The crude ethanol extract compounds inhibit MCM-B2 cell proliferation synergistically.

scholarly journals Efektivitas Kombinasi Ekstrak Etanol Daun Bunga Matahari dan Tanaman Anting-Anting sebagai Antimalaria Secara In Vivo

2019 ◽  
Vol 5 (1) ◽  
pp. 12-19 ◽  
Author(s):  
Khoirul Ngibad
Keyword(s):  
Medicinal Plants ◽  
Human Body ◽  
Antimalarial Activity ◽  
Combination Index ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Activity Test ◽  
Acalypha Indica

Sunflower (Helianthus annuus) and anting-anting (Acalypha indica Linn), can be studied as efficacious malaria medicinal plants for preventing the development of malaria plasmodium resistance. This study aims to determine the effectiveness of the combination of 80% ethanol extract of sunflower leaves and anting-anting as antimalarials. This study included extraction of sunflower leaves and anting-anting plant separately using the maceration method for 24 hours with 80% ethanol. Stirring is aided by a 3 hour shaker and then the obtained extracts were mixed and tested in vivo antimalarial activity against mice test animals. Data of the parasitemia degree was analyzed for the determination of Effective Dose (ED50) using probit analysis then determination of Combination Index (CI) value. The results of the antimalarial activity test on tested mice were ED50 of 1.23 mg/kg human body with a Combination Index (CI) of 0.46 (CI <1) which means synergistic potency.

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

scholarly journals In Vitro Cytotoxic Effects of Secondary Metabolites Present in Sarcopoterium Spinosum L.

2021 ◽  
Vol 11 (11) ◽  
pp. 5300
Author(s):  
Jozef Hudec ◽  
Jan Mojzis ◽  
Marta Habanova ◽  
Jorge A. Saraiva ◽  
Pavel Hradil ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Benzalkonium Chloride ◽  
Ethanol Extract ◽  
Cytotoxic Activities ◽  
Sarcopoterium Spinosum ◽  
Mcf 7

Sarcopoterium spinosum (L.) is a medicinal plant traditionally used for the treatment of various diseases including cancer in the Near- and Middle East. The fractions and constituents of the ethanol extract of S. spinosum were screened for in vitro cytotoxic activities on Jurkat (acute T-lymphoblastic leukemia), HeLa (cervical adenocarcinoma), MCF-7 (mammary gland adenocarcinoma), Caco-2 (human colorectal adenocarcinoma), and MDA-MB-231 (mammary gland adenocarcinoma) cell lines using the MTT (3-(dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The ethanol extract was subsequently re-extracted with ethyl acetate and in its sub-fraction obtained by column chromatography three compounds (stachydrine, benzalkonium chloride and rutine) were the first time identified by nuclear magnetic resonance (NMR) analyses. The most active subfraction showed cytotoxic activity against HeLa, MCF-7, and Caco-2 cell lines. The three compounds mentioned, as standards of high-performance liquid chromatography (HPLC) quality, were studied individually and in combination. Cytotoxic activity observed might be due to the presence of benzalkonium chloride and rutin. Benzalkonium chloride showed the strongest growth suppression effect against HeLa cells (IC50 8.10−7 M) and MCF-7 cells (IC50 5.10−6 M). The mixture of stachydrine and benzalkonium chloride allowed a synergistic cytotoxic effect against all tested cancer and normal cells to be obtained. Anti-cancer activity of the plant extract of S. spinosum remains under-investigated, so this research describes how the three major compounds identified in the ethyl acetate extract can exert a significant dose dependent in vitro cytotoxicity.

scholarly journals In vitro antioxidant and anticancer activity of crude ethanol extract of Mahkota Dewa (Phaleria macrocarpa) leaves

2021 ◽  
Author(s):  
Yuyun Ika Christina ◽  
Wirdatun Nafisah ◽  
Widodo ◽  
Muhaimin Rifa’i ◽  
Muhammad Sasmito Djati
Keyword(s):  
Anticancer Activity ◽  
Ethanol Extract ◽  
Phaleria Macrocarpa

Cyclopentadienyl‐Based Anticancer Drugs: Improvement of Cytotoxic Activity through Functionalisation of the π Ligand

ChemMedChem ◽  
2021 ◽  
Author(s):  
Monika Absolonová ◽  
Lucie Melounková ◽  
Jaromír Vinklárek ◽  
Jan Honzíček ◽  
Libor Dostál ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Anticancer Drugs

scholarly journals FORMULATION, STABILTY TEST AND ENZYME ACTIVITY TEST TYROSINASE OF CREAM PEPARATIONS FROM HERENDONG FRUIT EXTRACT(Melastoma affine D. Don)

2020 ◽  
Vol 5 (2) ◽  
pp. 149-173
Author(s):  
Zuraida Sagala ◽  
Kurnia Telaumbanua
Keyword(s):  
Enzyme Inhibitors ◽  
Enzyme Inhibitor ◽  
Mechanical Test ◽  
Ethanol Extract ◽  
Activity Test ◽  
Irritation Test ◽  
Ic50 Values ◽  
Tyrosinase Enzyme ◽  
Ethanol Extracts ◽  
The Stability

One way to prevent or inhibit melanin formation is by inhibiting tyrosinase activity (Lloyd,2011). Tyrosinase enzyme is an enzyme that plays a role in the formation of skin pigments or known as melanogenesis. In the process of melanogenesis, the enzyme tyrosinase acts as a catalyst in two different reactions, namely the hydroxylation process of tyrosine to dihydroxy-phenylalanine (L-DOPA) and oxidation of L-DOPA to quinone DOPA. Tyrosinase in skin tissue is activated by solar UV radiation so that it accelerates the process of melanin production (Fais et al.  2009).  This  study  was  conducted  to  determine  the  stability,  effectiveness  of  cream preparations from ethanol extracts of Harendong fruit (Melastoma affine D. Don) as tyrosinase enzyme inhibitors so it can be used as a cosmetic ingredient for whitening or skin lightening. Results Cream with variants of Harendong (Melastoma affine D. Don) ethanol extract was physically stable and formulated with organoleptic test, homogeneity, viscosity, mechanical test, pH, cycling test, and irritation test. Type M / A cream preparations from ethanol extract of Harendong fruit (Melastoma affine D. Don) have a strong / moderate tyrosinase enzyme inhibitor activity with IC50 values of F1 (1%) of 526,192 ppm, F2 (1.5%) ) amounted to 317,534 ppm and F3  (2%)  amounted  to  128,523  ppm.  Keywords: Foemulation,  stability test, activity test, tyrosinase enzyme, herenong fruit (Melastoma affine G.Don)

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