scholarly journals Stability Test of Gel Hand Sanitizer Ethanol Extract of Nutmeg (Pala) Leaves (Myristica fragrans Houtt.) with Variation of the Concentration of HPMC (Hydroxy Propyl Methyl Cellulose) and Glycerine

2021 ◽  
pp. 395-402
Author(s):  
Srie Rezeki Nur Endah ◽  
C Shintia ◽  
A Nofriyaldi
Keyword(s):  
Methyl Cellulose ◽  
Ethanol Extract ◽  
Stability Test ◽  
Gelling Agent ◽  
Cycling Test ◽  
Hand Sanitizer ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt

Daun pala (Myristica fragrans Houtt.) memiliki senyawa metabolit sekunder seperti flavonoid, saponin dan tanin. Oleh karena itu, daun pala dikembangkan ke dalam bentuk sediaan gel hand sanitizer yang berpotensi sebagai antibakteri. Dalam formulasi gel, penggunaan gelling agent dan humektan merupakan faktor penting yang dapat menghasilkan sediaan gel yang stabil. Tujuan dari penelitian ini yaitu untuk mengetahui stabilitas sediaan gel hand sanitizer ekstrak etanol daun pala dengan variasi konsentrasi HPMC dan gliserin. Gel hand sanitizer ini dibuat 3 formulasi dengan variasi HPMC dan gliserin, yaitu formula 1 (HPMC 1% : gliserin 5%), formula 2 (HPMC 1.5% : gliserin 7.5%), formula 3 (HPMC 2%, gliserin 10%). Uji stabilitas yang dilakukan terhadap sediaan menggunakan metode cycling test sebanyak 6 siklus. Berdasarkan hasil analisis cycling test 6 siklus dihasilkan bahwa formula yang memiliki stabilitas  paling baik adalah formula 1 dengan variasi konsentrasi HPMC 1% dan gliserin 5%.

scholarly journals PENGARUH VARIASI KONSENTRASI HPMC DAN GLISERIN TERHADAP SIFAT FISIK GEL HAND SANITIZER EKSTRAK ETANOL DAUN PALA (Myristica fragrans Houtt.)

2021 ◽  
Vol 4 (1) ◽  
pp. 58-69
Author(s):  
Citra Shintia ◽  
Srie Rezeki Nur Endah ◽  
Ali Nofriyaldi
Keyword(s):  
Methyl Cellulose ◽  
Gelling Agent ◽  
Hand Sanitizer ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt ◽  
One Way Anova

Daun pala (Myristica fragrans Houtt.) mengandung senyawa flavonoid, saponin dan tanin yang berpotensi sebagai antibakteri, sehingga dapat dimanfaatkan kedalam bentuk sediaan gel hand sanitizer. Persyaratan yang harus dipenuhi untuk sediaan gel salah satunya adalah sifat fisiknya. Gelling agent dan humektan merupakan faktor yang dapat mempengaruhi sifat fisik sediaan gel. Penelitian ini bertujuan untuk mengetahui pengaruh variasi konsentrasi HPMC (Hidroxy Propyl Methyl Cellulose) dan gliserin terhadap sifat fisik sediaan gel hand sanitizer ekstrak etanol daun pala. Gel hand sanitizer ini dibuat formulasi dengan variasi HPMC dan gliserin, yaitu formula 1 (HPMC 1% : gliserin 5%), formula 2 (HPMC 1,5% : gliserin 7,5%), formula 3 (HPMC 2%, gliserin 10%). Evaluasi yang dilakukan terhadap sediaan meliputi, uji homogenitas, uji organoleptik, uji pH, uji daya sebar, uji daya lekat. Berdasarkan hasil analisis menggunakan One Way Anova dengan program SPSS for Windows, menunjukan bahwa HPMC dan gliserin mempengaruhi parameter daya sebar dan daya lekat sediaan gel hand sanitizer ekstrak etanol daun pala (Myristica fragrans Houtt.).

scholarly journals Stability Test of Glycosaminoglycan and Achasin in Snail (Achatina fullica) Slime and Its Gel Formulation

2020 ◽  
Vol 10 (01) ◽  
pp. 5-8
Author(s):  
Fenita Shoviantari ◽  
Lia Agustina ◽  
Dimas Aditya
Keyword(s):  
Wound Healing ◽  
Protein Concentration ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Methyl Cellulose ◽  
Stability Test ◽  
Gelling Agent ◽  
Accelerate Wound Healing ◽  
Sds Page ◽  
The Stability

Snail slime (Achatina fullica) contains glycosaminoglycan, which can accelerate wound healing and achasin, which has antibacterial activity. The Objectives of the study to determine the stability of the protein in snail mucus in three different storage conditions. The snail slime stability test was carried out in two ways, namely the determination of protein content by the Lowry method and seeing the stability of the achasin and glycosaminoglycan proteins by the sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS PAGE) method. In addition, the formulation of snail slime gel was made with a variety of gelling agents, namely Hydroxy Propyl Methyl Cellulose (HPMC) and Carbomer. Protein concentration showed a decrease in protein concentration, but the SDS-PAGE result showed that the decrease was not inactive protein (glycosaminoglycan and achasin). Further study should be conducted to evaluate stability in long time with different excipient and also the effectiveness of formulation in accelerate wound healing. According to the result, we are observed, the snail slime was successfully formulated into a gel with HMPC and carbopol as a gelling agent.

scholarly journals EVALUASI SEDIAAN EMULGEL ANTI JERAWAT TEA TREE (Melaleuca alternifolia) OIL DENGAN MENGGUNAKAN HPMC SEBAGAI GELLING AGENT

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Lusi Nurdianti
Keyword(s):  
Methyl Cellulose ◽  
Gelling Agent ◽  
Tea Tree Oil ◽  
Melaleuca Alternifolia ◽  
Tea Tree ◽  
Cycling Test

<p><em>Tea tree (Melaleuca alternifolia) oil</em> memiliki terpinen-4-ol yang berfungsi sebagai antibakteri dan anti jamur yang memiliki potensi efektivitas mengobati jerawat. Penelitian ini bertujuan untuk mengetahui formulasi pembuatan sediaan dan evaluasi sediaan emugel <em>Tea tree (Melaleuca alternifolia) oil</em> dengan menggunakan <em>Hidroxy propyl methyl cellulose</em> (HPMC) sebagai <em>gelling agent.</em>Untuk memperoleh sediaan emulgel yang memiliki stabilitas baik maka dilakukan serangkaian tahapan penelitian mulai dari pengumpulan bahan baku, formulasi dan evaluasi sediaan emulgel <em>Tea tre oil</em>. Formulasi yang dibuat terdiri dari tiga formula yaitu F1, F2 dan F3 dengan variasi HPMC sebagai <em>gelling agent</em>. Evaluasi yang dilakukan adalah uji organoleptik, pH, homogenitas, daya sebar, viskositas, <em>Cycling test</em> dan uji hedonik. Analisis data hasil uji viskositas dan uji hedonik diolah dengan penjabaran deskriptif. Hasil uji hedonik menunjukan bahwa emulgel anti jerawat <em>tea tree oil</em> dapat diterima oleh responden.<em></em></p><p><strong> </strong></p><p><strong>Kata kunci</strong> : emulgel,<em> Melaleuca alternifolia</em>, HPMC<em></em></p>

scholarly journals FORMULATION AND ANTIBACTERIAL ACTIVITY TEST OF HAND SANITIZER ETHANOL EXTRACT OF BASIL (Ocimum Sanctum. L) AGAINST Escherichia coli dan Streptococcus Aureus

2020 ◽  
Vol 15 (2) ◽  
pp. 228
Author(s):  
Nurfiddin Farid ◽  
Nurhikma Nurhikma ◽  
Suhartina Hamzah ◽  
Muhammad Yusuf ◽  
Rahmania Rahmania
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Gelling Agent ◽  
Ocimum Sanctum ◽  
Hand Sanitizer ◽  
Activity Test ◽  
Before And After ◽  
And Staphylococcus Aureus

Basil leaves (Ocimum sanctum L) Against to inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria to increase its effectiveness, it is made in practical and easy-to-use preparations. One of the effective dosage forms for topical use is Hand Sanitizer. The factor that influences the quality of gel preparations is Carbopol as a gelling agent. To know the antibacterial effect and ethanol extract concentration of basis leaves (Ocimum sanctum L). preparation of Hand sanitizer, in which the selection and type of carbopol concentration will determine the stability of the gel formed. Basil leaf extract was obtained by maceration method using 96% ethanol solvent. The product is formulation in the form a gel Hand sanitizer with the addition of carbopol as a gelling agent with a concentration of 1,5% this type of research is  laboratory Experiment with Stability Test, Organoleptic Test, pH, Homogeneity and Scattering power and Antibacterial Test on Escherichia coli and Staphylococcus aureus. For testing antibacterial activity the method used is the diffusion method. Form the research results obtained antibacterial Hand sanitizer preparations with concentration of 1%, 3% and 5%  showed changes in odor , color and shape before and after storage at pH 4,8- 6,4. Organoleptic  testing showed no changes in odor, color and shape before and after accelerated storage. pH testing shows charges before and after storage are caused by temperature light factors. Homogeneity testing showed no changes before and after storage. Scatter tests indicate changes before and after storage caused by temperature light factors. Antibacterial activity test shows that the formulation of Hand sanitizer ethanol extract of basil leaves (Ocimum sanctum L) with a concentration of 1%, 3% and 5%. can inhibit the growth of Escherichia coli and Staphylococcus aureus with Medium -Strong inhibitory zones. From the results of research conducted it was concluded that the antibacterial Hand sanitizer ethanol of basis was physically and chemically stable for Organiletic, pH, Homogeneity and Scattering effects.

Uji Aktivitas Antioksidan Sediaan Masker Hidrogel Ekstrak Etanol Teh Hijau (Camellia sinensis) Dengan Metode BCB

2021 ◽  
Vol 1 ◽  
pp. 1059-1070
Author(s):  
Asri Septiani ◽  
W Wirasti ◽  
S Slamet ◽  
Urmatul Waznah
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Color Change ◽  
Ethanol Extract ◽  
Test Method ◽  
Stability Test ◽  
Cycling Test ◽  
The Stability ◽  
Β Carotene

AbstractGreen tea is a plant that contains antioxidants. The content of green tea compounds that act as antioxidants are polyphenols. The addition of natural antioxidants in hydrogel masks has the potential as an alternative to synthetic antioxidants. The purpose of this study was to determine the antioxidant activity of the hydrogel mask preparation of green tea (Camellia sinensis L.) ethanol extract. The method of determining antioxidant activity was carried out by the -Carotene Bleaching (BCB) method. The stability test of the preparation using the cycling test method was carried out for 6 cycles including organoleptic, viscosity, pH, homogeneity tests. The data obtained from the antioxidant activity test is the IC50 value. The IC50 results of the green tea ethanol extract were 27,162 ppm, while the IC50 values for the formulations 1, 2 and 3 were 40,893 ppm, respectively; 35,348 ppm; and 32,270 ppm. The results of the stability test showed that the preparation was stable from the parameters of viscosity, pH and homogeneity but not stable from the organoleptic parameters because there was a color change in the preparation containing the extract after the cycling test process. In conclusion, both extracts and hydrogel mask preparations contain high antioxidants with relatively stable dosage forms.Keywords: Green Tea, Antioxidants, Hydrogel Mask, -Carotene Bleaching (BCB), IC50. AbstrakTeh hijau merupakan salah satu tanaman yang mengandung antioksidan. Kandungan senyawa teh hijau yang berperan sebagai antioksidan adalah polifenol. Penambahan antioksidan alami pada masker hidrogel berpotensi sebagai alternatif pengganti antioksidan sintetis. Tujuan penelitian ini adalah mengetahui aktivitas antioksidan sediaan masker hidrogel ekstrak etanol teh hijau (Camellia sinensis L.). Metode penentuan aktivitas antioksidan dilakukan dengan metode β-Carotene Bleaching (BCB). Pengujian stabilitas sediaan dengan metode cycling test dilakukan sebanyak 6 siklus meliputi uji organoleptik, viskositas, pH, homogenitas. Data yang diperoleh dari uji aktivitas antioksidan berupa nilai IC50. Hasil IC50 ekstrak etanol teh hijau sebesar 27,162 ppm, sedangkan pada sediaan menunjukan nilai IC50 berturut-turut dari formulasi 1, 2 dan 3 sebesar 40,893 ppm; 35,348 ppm; dan 32,270 ppm. Hasil uji stabilitas menunjukkan sediaan stabil dari parameter viskositas, pH dan homogenitas tetapi tidak stabil dari parameter organoleptik karena terjadi perubahan warna pada sediaan yang mengandung ekstrak setelah proses cycling test. Kesimpulannya ekstrak maupun sediaan masker hidrogel mengandung antioksidan tinggi dengan bentuk sediaan relatif stabil.Kata kunci:Teh Hijau; Antioksidan; Masker Hidrogel; β-Carotene Bleaching (BCB);IC50.

scholarly journals EFEK PEMBERIAN EKSTRAK ETANOL BIJI PALA (Myristica fragrans Houtt) TERHADAP ANSIETAS MENCIT (Mus musculus)

2020 ◽  
Vol 2 (1) ◽  
pp. 36-46
Author(s):  
Irin N. Hasanusi ◽  
Paringotan Y. Silalahi ◽  
Johan B. Bension ◽  
Laura B.S. Huwae ◽  
Ony W. Angkejaya ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Control Group ◽  
Observation Data ◽  
Research Subjects ◽  
Control Group Design ◽  
Myristica Fragrans ◽  
Post Test ◽  
Myristica Fragrans Houtt ◽  
Negative Controls ◽  
Positive Controls

Anxiety is excessive worry without reason related to certain precipitating factors. The content of the compound myristicin and alkaloids contained in the nutmeg plant (Myristica fragrans Houtt) can be as antianxiety, so that its use can prevent the dependence effect of most antianxiety drugs. This study aims to determine the effect of nutmeg ethanol extract on anxiety on mice (Mus muculus). This research is a true experimental with post test only control group design. The research subjects were 30 mice which were taken by simple random technique and divided into the normal control groups (KN), negative controls (K-), positive controls (K+), ethanol extract of nutmeg seeds at a concentration of 4% (P1), and ethanol extract of nutmeg seeds at a concentration of 16% (P2). In the group K-, K +, P1, and P2 were given stress treatment with the FST method for 6 minutes. Then in the K+ group were given a dose of alprazolam is 0.2 ml, P1 were given ethanol extract of nutmeg seeds at a concentration of 4% and P2 were given ethanol extract of nutmeg seeds at a concentration of 16%. The treatment is carried out for 7 days and on the 8th day will be tested with EPM. Observation data were analyzed using the ANOVA test. The results showed that the administration of ethanol extract of nutmeg seeds had a significant effect on the length of time the mice were in the open arms of EPM (p<0,05). The decrease of anxiety levels in the P1 and P2 mice in this study was due to the content of the myristicin, flavonoid and saponin compounds found in ethanol extract of nutmeg seeds.

Phytochemical and Pharmacological Activity of Myristica fragrans Houtt (Myristicaceae)

2017 ◽  
Vol 9 (01) ◽  
Author(s):  
Md Arifur Rahman Chowdhury ◽  
Manirujjaman . ◽  
Md Mazedul Haq
Keyword(s):  
Acetic Acid ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Writhing Test ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt

Objective: Myristica fragrans Houtt commonly used as traditional medicine for alleviating of various disorders. The purpose of our study was to map out the in vitro antioxidant property and in vivo anti-hyperglycemic and analgesic effect of the methanolic extract of Myristica fragrans Houtt. (Seed and mace) (Myristicaceae) on Swiss albino mice. Methods: The processed powder of Myristica fragrans Houtt (seed and mace) were subjected to methanolic extraction by soxhlet filtration methods, and the desiccated extract was used for screening of antioxidant by DPPH free radical scavenging assessment as well as total phenolic content by using folin-ciocalteu reagent.Anti-hyperglycemic effect and analgesic action tested through alloxan induced antidiabetics test and acetic acid-tempted writhing test on mice. Results: In DPPH free radical scavenging assessment, free radicals neutralization expressed as % of inhibition 49.69±0.06% also by IC50 values as 68.43 µg/ml surmise middle level of antioxidant property. The total phenolic content expressed as 186.25 mg/g equivalent of gallic acid indicates, active phenolic content. Oral administration of 200 and 400 mg/kg of extract dose and reference drug vildagliptin (50 mg/kg) for the duration of the 4-day study period, and initiated % of inhibition the blood glucose level measured as 22.48%, 44.78% and 62.02% regard as the significant anti-hyperglycemic properties. The analgesic activity was investigated by using the acetic acid-induced writhing test in mice, at the dose of 200 mg/kg body and 400mg/kg weight, and resulting 50.4% and 68.10% correspondingly, which was considerably significant with a standard drug. Conclusion: The present study suggests that methanolic extract of seed and mace of Myristica fragrans Houtt can manage moderate oxidative stress as well as perform the painkilling action. Besides, prolong medication may enhance the new dimension of anti-hyperglycemic activity.

Karekteristik Mikrokapsul Oleoresin Fuli Pala (Myristica Fragrans Houtt) Yang Dienkapsulasi Dengan Gum Arab

2020 ◽  
Vol 14 (2) ◽  
pp. 1
Author(s):  
Alston Millan
Keyword(s):  
Scavenging Activity ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt

<p align="center"> </p><p align="center"><strong>ABSTRAK</strong></p><p align="center"><strong> </strong></p>Tujuan<strong> </strong>penelitian ini adalah untuk mengetahui pengaruh formulasi berbagai total padatan yang terdiri dari gum Arab sebagai enkapsulan dan oleoresin fuli pala sebagai core terhadap karakteristik mikrokapsul yang dihasilkan. Variasi formulasi oleoresin fuli pala dan gum arab yang dicobakan adalah A1 (5%), A2 (10%), A3 (15%), A4 (20%) dan A5 (20%). Pembentukan emulsi dilakukan dengan <em>Turrax homogenizer</em> dengan kecepatan 4000 rpm selama 5 menit. Pembentukan mikrokapsul dilakukan dengan menggunakan pengering semprot (<em>spray</em> <em>dryer</em>) dengan suhu inlet dan outlet masing-masing 110<sup>0</sup>C dan 62<sup>0</sup>C. Mikrokapsul yang dihasilkan dianalisis karakteristiknya yang meliputi rendemen mikrokapsul, kadar oleoresin terkapsulkan, kadar oleoresin tak terkapsulkan, kadar air, <em>engel of repose,</em> <em>wettability</em> dan rehidrasi, aktivitas antioksidan dengan metode <em>RSA (radikal scavenging activity) DPPH (2-2Dhpypenil-2 Picrylhydrazil)</em> dan kromatografi gas-spektrometri massa (GC-MS). Mikrokapsul yang terbaik diperoleh dengan variasi formulasi oleoresin fuli pala dan gum arab adalah  A1 (5%). Sifat mikrokapsul yang diperoleh menpunyai rendemen mikrokapsul 24,68%, oleoresin terkapsulkan 92,68%, oleoresin tak terkapsulkan 7,607%, kadar air 8,444%, <em>angel of repose</em> 19,083 (<sup>0</sup>), <em>wettability</em> 5,9 (menit), aktivitas antioksidan (IC<sub>50)</sub> 1032 ppm dan kromatografi gas-spektrometri massa (GC-MS) dengan komponen terbesar penyusun mikrokapsul oleoresin fuli pala yaitu <em>sabinene hexane, 4-methylene-1-, myristycin, phenol 2,6 dimetoksi-4-2 (2-peopenil), phenol, 2-metoksi-4-(propennyl).</em>

Sign in / Sign up

Export Citation Format

Share Document