scholarly journals EFEK PEMBERIAN EKSTRAK ETANOL BIJI PALA (Myristica fragrans Houtt) TERHADAP ANSIETAS MENCIT (Mus musculus)

2020 ◽  
Vol 2 (1) ◽  
pp. 36-46
Author(s):  
Irin N. Hasanusi ◽  
Paringotan Y. Silalahi ◽  
Johan B. Bension ◽  
Laura B.S. Huwae ◽  
Ony W. Angkejaya ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Control Group ◽  
Observation Data ◽  
Research Subjects ◽  
Control Group Design ◽  
Myristica Fragrans ◽  
Post Test ◽  
Myristica Fragrans Houtt ◽  
Negative Controls ◽  
Positive Controls

Anxiety is excessive worry without reason related to certain precipitating factors. The content of the compound myristicin and alkaloids contained in the nutmeg plant (Myristica fragrans Houtt) can be as antianxiety, so that its use can prevent the dependence effect of most antianxiety drugs. This study aims to determine the effect of nutmeg ethanol extract on anxiety on mice (Mus muculus). This research is a true experimental with post test only control group design. The research subjects were 30 mice which were taken by simple random technique and divided into the normal control groups (KN), negative controls (K-), positive controls (K+), ethanol extract of nutmeg seeds at a concentration of 4% (P1), and ethanol extract of nutmeg seeds at a concentration of 16% (P2). In the group K-, K +, P1, and P2 were given stress treatment with the FST method for 6 minutes. Then in the K+ group were given a dose of alprazolam is 0.2 ml, P1 were given ethanol extract of nutmeg seeds at a concentration of 4% and P2 were given ethanol extract of nutmeg seeds at a concentration of 16%. The treatment is carried out for 7 days and on the 8th day will be tested with EPM. Observation data were analyzed using the ANOVA test. The results showed that the administration of ethanol extract of nutmeg seeds had a significant effect on the length of time the mice were in the open arms of EPM (p<0,05). The decrease of anxiety levels in the P1 and P2 mice in this study was due to the content of the myristicin, flavonoid and saponin compounds found in ethanol extract of nutmeg seeds.

scholarly journals JENIS LEUKOSIT MENCIT (Mus musculus) PASCA STRES AKUT DENGAN PERLAKUAN EKSTRAK ETANOL BIJI PALA (Myristica fragrans Houtt)

2021 ◽  
Vol 2 (2) ◽  
pp. 103-116
Author(s):  
Zulhaimi Hendrajid ◽  
Yuniasih M. J. Taihuttu ◽  
Parningotan Y. Silalahi ◽  
Laura B. S. Huwae ◽  
Vina Z. Latuconsina
Keyword(s):  
Interferon Gamma ◽  
Forced Swim Test ◽  
Control Group ◽  
T Helper ◽  
Control Group Design ◽  
Group Design ◽  
Swim Test ◽  
Myristica Fragrans ◽  
Post Test ◽  
Myristica Fragrans Houtt

Glukokortikoid merupakan salah satu jenis hormon stres yang akan menekan respons imun normal dengan jalan memblokade program sel T helper satu yang merupakan penghasil interferon gamma. Glukokortikoid dapat memberikan dampak terhadap penurunan bobot badan, penurunan sistem imunitas tubuh, dan perubahan diferensiasi leukosit. Pala (Myristica fragrans Houtt) adalah tanaman tradisional Maluku dan mengandung senyawa metabolit sekunder yang dapat bersifat sebagai imunomodulator. Penelitian ini bertujuan untuk mengetahui jumlah dan jenis leukosit mencit yang diinduksi stres dan diberi ekstrak etanol biji pala. Penelitian ini merupakan eksperimen murni dengan desain post test only control group design. Mencit sebanyak 30 ekor di bagi menjadi lima kelompok perlakuan yaitu kelompok kontrol normal (KN), kontrol negatif (K-), kontrol positif (K+), ekstrak etanol biji pala konsentrasi 4% (P1) dan 16% (P2). Pada K-, K+, P1 dan P2 diberi perlakuan stres dengan metode FST (Forced Swim Test) selama enam menit. Kemudian, pada kelompok K+ diberi obat alprazolam dosis 0,2 ml, P1 dan P2 diberi ekstrak etanol biji pala sebanyak 0.5 ml. Perlakuan diberikan selama 7 hari dan pada hari ke-8 dilakukan koleksi darah mencit intrakardial. Data hasil pengamatan dianalisis dengan ANOVA. Hasil penelitian menunjukkan kelompok P1 dapat menurunkan persentase neutrofil, meningkatkan persentase monosit dan limfosit, sedangkan pada P2 dapat menurunkan persentase neutrofil dan monosit, meningkatkan persentase limfosit dan tidak berpengaruh terhadap persentase eosinofil dan basofil. Sehingga dapat disimpulkan bahwa pemberian ektsrak etanol biji pala tidak berpengaruh signifikan terhadap rata-rata persentase neutrofil, monosit, limfosit, eosinofil dan basofil (p>0,05).

scholarly journals Uji daya hambat ekstrak buah pala (myristica fragrans Houtt) terhadap bakteri penyebab periodontitis porphyromonas gingivalis secara in vitro

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Pricillia T. Kaawoan ◽  
Jemmy Abidjulu ◽  
Krista V. Siagian
Keyword(s):  
Porphyromonas Gingivalis ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Myristica Fragrans ◽  
Post Test ◽  
Myristica Fragrans Houtt

Abstract: Periodontal disease is preceded by a buildup of plaque that contains a collection of bacteria. The most common bacteria found in plaques are Porphyromonas gingivalis that cause periodontitis. There are several ways to treat periodontitis inter alia the usage of natural materials. Nutmeg (Myristica fragrans Houtt) contains volatile oil, saponins, and alkaloids known as antibacterials. This study aimed to investigate the inhibitory effect of nutmeg extract on Porphyromonas gingivalis. This was an experimental laboratory study in vitro with a post test only control group design. The testing method used in this study was a modified method of Kirby-bauer. Nutmeg was extracted by using maceration method with ethanol 96%. Porphyromonas gingivalis bacteria were ordered from University of Hasanuddin Makassar and then were rejuvenated in the Laboratory of Microbiology Pharmacy FMIPA University of Sam Ratulangi Manado. The results showed that the average inhibitory zone of nutmeg extract was 13,5 mm. Conclusion: Nutmeg (Myristica fragrans Houtt) extract had an inhibitory effect on the Porphyromonas gingivalis bacteria.Keywords: nutmeg (Myristica fragrans Houtt), inhibition zone, periodontitis, Porphyromonas gingivalisAbstrak: Penyakit periodontal berawal dari penumpukan plak yang mengandung kumpulan bakteri. Bakteri yang paling banyak ditemukan yaitu bakteri Porphyromonas gingivalis yang menyebabkan penyakit periodontitis. Terdapat beberapa cara untuk mengobati periodontitis, salah satunya dengan penggunaan bahan alami. Pala (Myristica fragrans Houtt) memiliki kandungan minyak atsiri, saponin, dan alkaloida yang diketahui berefek antibakteri. Penelitian ini bertujuan untuk mengetahui adanya daya hambat ekstrak buah pala terhadap bakteri penyebab periodontitis Porphyromonas gingivalis. Jenis penelitian ini ialah eksperimental laboratorik secara in vitro dengan post test only control group design. Metode pengujian yang digunakan yaitu modifikasi Kirby-bauer menggunakan sumuran. Sampel buah pala diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri Porphyromonas gingivalis yang digunakan dalam penelitian ini dikirim dari Universitas Hasanuddin Makassar yang telah diremajakan di Laboratorium Mikrobiologi Farmasi FMIPA Universitas Sam Ratulangi Manado. Hasil penelitian mendapatkan zona hambat ekstrak buah pala sebesar 13,5 mm. Simpulan: Ekstrak buah pala (Myristica fragrans Houtt) mempunyai daya hambat terhadap bakteri penyebab periodontitis Porphyromonas gingivalis.Kata kunci: pala (Myristica fragrans Houtt), zona hambat, periodontitis, porphyromonas gingivalis

Pengaruh Endorphin Massage terhadap Nyeri Haid Mahasiswi Tingkat Akhir S1 Keperawatan Universitas Nahdlatul Ulama Surabaya

2019 ◽  
Vol 4 (2) ◽  
Author(s):  
Yurike Septianingrum ◽  
Nety Mawarda Hatmanti
Keyword(s):  
Nursing Students ◽  
Design Research ◽  
Intervention Group ◽  
Simple Random Sampling ◽  
Control Group ◽  
Research Subjects ◽  
Menstrual Pain ◽  
Control Group Design ◽  
Vas Score ◽  
Post Test

Introduction: Menstrual pain or dysmenorrhea is a common problem that is often complained of by women during menstruation. Menstrual pain can interfere learning activities, especially final year students in completing their thesis. The purpose of this study were to analyze the effect of endorphin massage on menstrual pain in final year nursing students of UNUSA. Method: This study using an experimental with pre-test and post-test control group design. Research subjects of this study was recruited using simple random sampling who was included inclusion and exclusion criteria. Research subjects in this study was of 46 students who were then divided into two groups, 23 students into intervention group and 23 students into control group. Data were collected by using observation with VAS. Data were analyzed by using Wilcoxon and Mann-Whitney U test. Result and Analysis: The results showed that: 1) There was difference VAS score between pre test and post test in intervention group (p= 0,000), 2) There was difference VAS score between intervention and control group (p= 0,017). Conclusion: Endorphin massage could reduce menstrual pain in final year nursing student of UNUSA. Further research, is expected that endorphin massage can be compared with music therapy to reduce menstrual painKeywords: endorphin massage, menstrual pain, primary dysmenorrhea

scholarly journals PELATIHAN PUKULAN BAYANGAN LEBIH BAIK DARI PADA PUKULAN BIASA DALAM MENINGKATKAN VO2MAX DAN DAYA TAHAN OTOT LENGAN PADA ATLET TINJU AMATIR KABUPATEN LOMBOK TENGAH (NTB)

2019 ◽  
Author(s):  
Khaerul Anam ◽  
I P G Adiatmika ◽  
I Putu Adiartha Griadhi ◽  
I Made Muliarta ◽  
Luh Putu Ratna Sundari ◽  
...  
Keyword(s):  
Treatment Group ◽  
Anaerobic Capacity ◽  
T Test ◽  
Control Group ◽  
Research Subjects ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Arm Muscles ◽  
Test Control

Boxing is the basic gesture for all martial arts, because all of them definitely use the shadow shot and common shot. These two types of shots are related each other because anaerobic capacity must go along with aerobic capacity.To attain the proper knowledge about the method of shadow shot and common shot in increasing VO2max for the endurance of arm muscles for boxer athletes in Central Lombok Regency. Research was experimental with pre-test and post-test control group design, this research wasconducted in Pertina Lombok Tengah Regency. The research subjects involved 15 people divided into two groups, 7 and 8 samples respectively. The control group given only shadow shot while treatment group given common shot. This training held 3 times a week in six weeks for both groups. The analyses data with independent t-test. Researcher found The endurance of arm muscles (p<0,05) that training in twu group his’not significant to VO2max (p>0,05).Keywords: Shadow blows, regular blows, Boxing, VO2max and Endurance of arm Muscl

scholarly journals UJI EFEKTIFITAS BIOLARVASIDA EKSTRAK ETANOL BUAH LAMPESU (Baccaurea lanceolata) TERHADAP LARVA INSTAR III Culex quinquefasciatus

2020 ◽  
Vol 3 (3) ◽  
pp. 7
Author(s):  
Dea Alfani Nandjan
Keyword(s):  
Culex Quinquefasciatus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Chemical Insecticide ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Infection Disease

Filariasis is chronic infection disease caused by worm and carried by Culex quinquefasciatus mosquito. One of theways to controlling mosquitoes vectors are use biolarvacide or chemical insecticide. Chemical insecticide causingresistance Culex quinquefasciatus larvae and toxic for human. This research aim to determine the biolarvacideeffectiviteness of ethanol extract lampesu fruit (Baccaurea lanceolata) to Culex quinquefasciatus larvae instars III.This study was true experimental with a Post test-only control group design. This study used 700 larvae instars III ofCulex quinquefasciatus divided into 7 groups consentration of 0,2%, 0,4%, 0,6%, 0,8% and 1%, the positive control(abate) and negative control (aquadest). The observation was did after treatment in 3 hours, 6 hours, 12  hours and 24hours. The experiment is replicated four times. At 24 hours exposure concentration of 0,6% the test larvae mortalityreached 38% and at concentration of 1% the test larvae mortality reached 30%. In this stuy the concentration of1,531 % was effective to kill larvae with of 50% mortality(LC50) and concentration of 10,729 % was effective to killlarvae with of 90% mortality(LC90). Ethanol Ekstract of Lampesu Fruit (Baccaurea lanceolata) not effective asbiolarvacide ofCulex quinquefasciatus larvae instars III.

scholarly journals EFEK EKSTRAK ETHANOL DAUN KELOR (MORINGA OLEIFERA) TERHADAP PROTEKSI FUNGSI HATI DAN HISTOPATOLOGI TIKUS PUTIH (RATTUS NORVEGICUS) YANG DIINDUKSI KARBONTETRAKLORIDA (CCL4)

2021 ◽  
Vol 21 (1) ◽  
pp. 177-185
Author(s):  
Siti Hardiyanti Nawir ◽  
Peter Kabo ◽  
Ilhamjaya Pattelongi
Keyword(s):  
Liver Function ◽  
Rattus Norvegicus ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Blood Chemistry ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Moringa Oleifera Leaves ◽  
One Way Anova

Abstrak Kerusakan hati menjadi salah satu penyakit dengan prevalensi yang tinggi khususnya di negara berkembang. Penelitian ini bertujuan untuk melihat adanya efek ekstrak ethanol daun kelor (Moringa oleifera) terhadap proteksi fungsi hati dan histopatologi tikus putih (Rattus norvegicus) yang diinduksi karbontetraklorida (ccl4). serta menentukan dosis efektif dari kombinasi tersebut. Penelitian ini dilakukan secara eksperimental laboratorium menggunakan metode eksperimen dengan desain post test only control group pada tikus wistar. Tikus dibagi menjadi 5 kelompok, yaitu kelompok kontrol positif (ekstrak kurkuma), kontrol pembanding (NaCMC 1%) dan 3 kelompok pemberian ekstrak dengan 3 variasi dosis masing-masing 250 mg/kgBB, 500 mg/kgBB dan 750 mg/kgBB. Dilakukan pengamatan terhadap uji profil kimia darah yang meliputi SGOT dan SGPT serta dilanjutkan dengan uji histopatologi. Hasil menunjukkan bahwa adanya perbedaan perubahan hasil pengukuran darah fungsi hati (SGOT dan SGPT) antar kelompok selama 8 hari. Hasil menunjukkan bahwa adanya perbedaan perubahan hasil pengukuran profil kimia darah antar kelompok selama perlakuan. Berdasarkan hasil uji one way ANOVA pada pengukuran SGOT dan SGPT terdapat hasil tes bermakna (P<0,05). Hasil uji histopatologi menunjukkan bahwa terdapat perbaikan fungsi dan struktur hati pada kelompok pemberian ekstrak etanol daun kelor. Dapat disimpulkan bahwa ekstrak etanol daun kelor (Moringa oleifera) memiliki kemampuan sebagai hepatoprotektor terhadap fungsi hati pada dosis 750 mg/kgBB. Abstract Liver damage is one of the diseases with high prevalence especially in developing countries. This study aims to see the effect of ethanol extract of Moringa oleifera leaves on liver function protection and histopathology of white rat (Rattus norvegicus) induced by carbontetrachloride (CCl4). and determine the effective dose of the combination. This research was conducted experimentally using a laboratory experimental method with a post test only control group design in wistar rats. Rats were divided into 5 groups, namely the positive control group (curkuma extract), comparative control (NaCMC 1%) and 3 groups giving extracts with 3 variations of each dose of 250 mg / kgBB, 500 mg / kgBB and 750 mg / kgBB. Observations were made on the blood chemistry profile test which included SGOT and SGPT and continued with histopathological testing. The results showed that there were differences in changes in the results of liver function blood measurements (SGOT and SGPT) between groups for 8 days. The results showed that there were differences in changes in the measurement results of blood chemistry profiles between groups during treatment. Based on the results of the one way ANOVA test on SGOT and SGPT measurements there were significant test results (P <0.05). The results of histopathological tests showed that there was an improvement in liver function and structure in the group given ethanol extract of kelor leaves. It can be concluded that the ethanol extract of Moringa oleifera leaves has the ability as a hepatoprotector of liver function at a dose of 750 mg / kgBB.

scholarly journals Pengaruh Musik Latar Terhadap Kenyamanan Kerja Karyawan Bank BCA

PROMUSIKA ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 69-76
Author(s):  
M. Alfiah Akbar
Keyword(s):  
Work Environment ◽  
T Test ◽  
Background Music ◽  
Control Group ◽  
Research Subjects ◽  
Control Group Design ◽  
Management And Development ◽  
Post Test ◽  
Quasi Experimental ◽  
Test Control

Pembinaan dan pemeliharaan lingkungan kerja yang nyaman merupakan sasaran yang penting dalam kaitannya dengan pengelolaan dan pengembangan sumber daya manusia dalam perusahaan. Salah satu upaya untuk menciptakan kenyamanan kerja karyawan adalah dengan mendengarkan musik, karena musik dapat memberikan suasana baru bagi lingkungan kerja. Tujuan dari penelitian ini adalah untuk menguji hipotesis “Ada peningkatan kenyamanan kerja pada karyawan BCA saat diperdengarkan musik latar” dan mengidentifikasi tingkat kenyamanan kerja karyawan setelah mendengarkan musik serta mengidentifikasi jenis musik latar yang dapat mendukung suasana kenyamanan kerja karyawan BCA. Metode yang digunakan dalam penelitian ini adalah kuantitatif dengan pendekatan kuasi eksperimen. Adapun desain kuasi ekperimen yang digunakan adalah Pre-test Post-test Control Group Desain. Subjek penelitian adalah 30 orang karyawan Divisi Keuangan Bank BCA, 15 orang kelompok kontrol (KK) dan 15 orang kelompok eksperimen (KE). Pengumpulan data dalam penelitian ini menggunakan kuesioner kenyamanan kerja, Hasil perhitungan menggunakan t-test menunjukkan nilai t sebesar 0,113 (p > 0,05), maka Ho diterima dan Ha ditolak. Hal tersebut berarti bahwa tidak terdapat peningkatan kenyamanan kerja yang signifikan pada kelompok kontrol. Selanjutnya melakukan uji nilai pretest dan posttest pada kelompok eksperimen. Hasil perhitungan menggunakan t-test menunjukkan nilai t sebesar 0,01  (p < 0,05), maka Ha diterima dan Ho ditolak. Berdasarkan hasil analisis tersebut, dapat disimpulkan bahwa terdapat peningkatan kenyamanan kerja yang signifikan pada kelompok eksperimen. Fostering and maintaining a comfortable work environment is an important target in relation to the management and development of human resources in the company. One effort to create employee work comfort is to listen to music, because music can provide a new atmosphere for the work environment. The purpose of this study is to test the hypothesis "There is an increase in work comfort for BCA employees when playing background music" and identify the level of work comfort of employees after listening to music and identify the type of background music that can support the comfort atmosphere of BCA employee work. The method used in this research is quantitative with a quasi-experimental approach. The quasi-experimental design used is the Pre-test Post-test Control Group Design. The research subjects were 30 employees of the Bank BCA Finance Division, 15 control groups (KK) and 15 experimental groups (KE). Collecting data in this study using a work comfort questionnaire, the results of calculations using the t-test showed a t value of 0.113 (p> 0.05), then Ho was accepted and Ha was rejected. This means that there is no significant increase in work comfort in the control group. Next test the pretest and posttest values in the experimental group. The results of calculations using the t-test showed a t value of 0.01 (p <0.05), then Ha was accepted and Ho was rejected. Based on the results of the analysis, it can be concluded that there is a significant increase in work comfort in the experimental group.Keywords: Background music, work comfort, moods, emotions

scholarly journals PENGARUH KONSELING GIZI DENGAN MEDIA BOOKLET TERHADAP KONSUMSI SAYUR BUAH DAN FAST FOOD PADA REMAJA OBESITAS

2019 ◽  
Vol 7 (2) ◽  
pp. 115-119
Author(s):  
Neldawati Ningrum ◽  
Ria Ambarwati ◽  
Enik Sulistyowati
Keyword(s):  
Fast Food ◽  
Vegetable Consumption ◽  
Control Group ◽  
Nutrition Counseling ◽  
Research Subjects ◽  
Control Group Design ◽  
Fast Food Consumption ◽  
Post Test ◽  
Vegetables And Fruits ◽  
The Impact

Background: Several factors cause obesity in adolescence are the lack of consumption of vegetables, fruits and excessive consumption of fast food. Nutrition counseling about benefits of vegetables, fruits and the impact of fast food on obesity is an effort that would be increase consumption of vegetables, fruits and reduce consumption of fast food.Objective: To determine the effect of counseling with booklet media on consumption of vegetables, fruits and fast food in obese adolescents at SMP N 40 Semarang.Method: The type of research is a true experiment with randomized pre and post test control group design. The research subjects consisted of 15 intervention samples who took nutrition counseling treatment with booklet media and 15 control samples were only given booklet media without nutritional counseling. The data was analysed by Independent T Test and Mann Whitney.Results: Nutrition counseling with booklet increased vegetable consumption (0,37 portion) and decreased fast food consumption (1,09 portion) but the result were not significant in the statistical test (p 0.05). However, nutrition counseling with booklet significantly (p 0.05) increased fruit consumption (0,63 portion).Conclusion: Nutrition counseling with booklet increased consumption of vegetables and fruits, and also reduce consumption of fast food.

scholarly journals The Effect of Co-Administration of Telang Leaves Ethanolic Extract Towards Fluoxetin’s Sedative Effect on Male Balb/C Mice Based on Sleeping Duration Parameter

2017 ◽  
Vol 6 (1) ◽  
pp. 7
Author(s):  
Anif Nur Artanti ◽  
Maria Ulfah
Keyword(s):  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Sedative Effect ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Control Group Design ◽  
Group Design ◽  
Sleep Analysis ◽  
Post Test ◽  
The Mean

One of Telang plant’s advantages is its activity as sedative agent. Previous studies indicate that Telang plant has anticonvulsant activity on mice at certain dose. This study was conducted to find out the sedative effect of Telang plant, particularly its leaf, when being co-administered with fluoxetin. Phytochemical analysis was carried out qualitatively on Telang ethanolic extract leaves (TEE) to find out the content of chemical compound first.This study was an experimental research with post-test only control group design, employing male balb/c mice. The effect of Telang ethanolic extract co-administration with fluoxetin was observed. The parameter being used in the study was the duration of sleep. Analysis was done by comparing mice sleeping duration prior to administration of fluoxetin alone and in combination with Telang ethanolic extract. The data was then analyzed using SPSS 17.0 for Windows.The results showed that TEE contained tannin, saponin, and flavonoid compounds, and co-administration of TEE and fluoxetin at various doses could provide sedative effect on mice. The co-administration of 400 mg/kgBW extract and 15 mg/kgBW fluoxetin could provide the mean sleeping duration 43 minutes longer than positive control.Keywords:  telang leaves ethanol extract, fluoxetin, sleeping duration

scholarly journals Effectivity Test of 96% from Soe (Citrus sinensis L.) Sweet Orange Rind Ethanol Extract as Biolarvaside

2019 ◽  
Vol 17 (2) ◽  
pp. 176-183
Author(s):  
Kuntum Ekawati Nurdin ◽  
Loisa Ratna Yuvita Olla ◽  
Stefany Febiand Feoh ◽  
Anita Dwi Putri Galla ◽  
Khusnul Dwi Istnaini ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Citrus Sinensis ◽  
Sweet Orange ◽  
Ethanol Extract ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Minimum Concentration ◽  
Post Test ◽  
Orange Fruit

One of the plants that can be used as larvicides is sweet orange (Citrus sinensis). Sweet orange rind containing saponins, tannins, flavonoids, and triterpenoids have a characteristic of aromatic odor and bitter taste which contain 96% essential oils containing limonene, glucoside, hesperidium, and resin that can function as biolarvasides. The Regency of the Middle East South is one of the areas in Indonesia which is known as the center of sweet orange. Soe sweet orange fruit has a distinctive color, aroma, and taste compared to other oranges in Indonesia. The objectives of this study are to discover the effectiveness of the orange rind extract in killing the Aedes aegypti larvae and to identify the minimum concentration of the extract of the sweet, dry rind in killing the larvae. This type of research is experimental post-test only control group design. This research was conducted at the Health Analyst laboratory of Kupang Poltekkes (Health Polytechnic) in January 2019. The third (III) instar larvae were placed in 5 vials, each containing 15 larvae. The total number of samples needed was 375 larvae. It was mixed with 15 ml of Soe sweet orange rind with a concentration of 0.075%, 0.1%, 0.25%, 0.50%, 0.75%. One-Way Anova test results obtained sig p-value=0,000 (p<0.05), meaning that there is an influence of sweet orange rind ethanol extract on the death of larvae or orange rind extract is effective as a natural insecticide. Significant values ​​were obtained for all concentrations p=0.008 (p> 0.05) meaning that there was an average difference of each concentration. The conclusion of the study shows that the ethanol extract of sweet orange rind (Citrus sinensis L.) is effective in killing Aedes aegypti mosquito larvae with a minimum concentration of ethanol extract sweet orange rind (Citrus sinensis L.) which can kill 0.075% of larvae.

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