Identification of Dendrobium (Orchidaceae) in Liwa Botanical Garden Based on Leaf Morphological Characters

Orchid is one of the most popular ornamental plants in the world. One of the orchid genera that is collected in a large number and known to have high morphological variations in the Liwa Botanical Garden is Dendrobium. However, to date, many Dendrobium collections have not been identified. Given the urgency of identification and the limitations of specimens in the field, especially flower organs, this study is important. This study aims to determine variations in morphological characters, phenetic relationships, and to identify Dendrobium collections based on leaf morphological characters in the Liwa Botanical Garden. Five accessions of Dendrobium were collected, namely CAT140, CAT 144, CAT 271, CAT 274, and IR015. Observation of 11 morphological characters leaves showed that leaf had high variations. The phenetic relationship based on the Gower similarity value and the UPGMA method shows that the Dendrobium in the Liwa Botanical Garden can be classified into 2 main groups formed with a similarity index value of 0.813. Based on Principle Component analysis values, it is known that the characters that have a large influence on grouping are the ratio of leaf length and width, leaf cross section, and leaf arrangement. The phenetic dendrogram topology is supported by the morphological character classification. The results of this study are expected to be basic information in the identification of natural orchids and conservation efforts in the Liwa Botanical Garden.

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BIODIVERSITICAL CHARACTERIZATION AND BIOCHEMICAL EVALUATION OF NATURALIZED SHRUB, ATI BALA (ABUTILON INDICUM) IN AGRA (UP) INDIA

FLORA AND FAUNA ◽

10.33451/florafauna.v24i1pp53-57 ◽

2018 ◽

Vol 24 (1) ◽

Author(s):

ATUL TIWARI

Keyword(s):

In Situ Conservation ◽

Similarity Index ◽

Distribution Patterns ◽

Botanical Garden ◽

Wiener’S Index ◽

The Status ◽

Biochemical Evaluation ◽

Index Value ◽

Natural Herb ◽

Abutilon Indicum

For the study of Biodiversity characterization and Biochemical analysis, the natural herb was transplanted at Botanical Garden, R.B.S. College, Agra. For the study of species diversity at four natural sites of Agra district, four formulae were used to study the maximum and minimum diversity of species at its natural sites. Shannon Wienner’s Index, Menhinick’s Index, Margalef’s Index value at four different sites was given in Table -1. These values determined the biodiversity variation at natural sites in Abutilon indium. The values of Moisture contents (%), Oil content (%) and Protein concentration (μ”/ml) in Abutilon indicum were also determined with variation at biochemical level Table -2. With the help of SDS-PAGE, in four samples of seeds of Abutilon indicum from four natural sites ( Bichpuri site -Lane 1, Kailash site -Lane 2, Kheria site - Lane 3, Chhalesar site - Lane 4). The total no. of protein bands showed diversity in Abutilon indicum plant in Table: 3. Shannon- Wiener’s Index, Menhinick’s Index, Margalei’s Index and Similarity Index (S) were applied to determine the distribution patterns and concentrations of wild species to pinpoint the diversity built-up and variation in the gene pool and identifying sites for in-situ conservation. The present work determined the status of diversity of experimental herb at particular four sites the need to conserve this diversity for current and future use.

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Molecular phylogeny of the sect. Adonis (Genus adonis L., Ranunculaceae)

Genetika ◽

10.2298/gensr2003075n ◽

2020 ◽

Vol 52 (3) ◽

pp. 1075-1086

Author(s):

Fatemeh Najariyan ◽

Masoud Sheidai ◽

Fahimeh Koohdar ◽

Mehdi Talebi

Keyword(s):

Molecular Phylogeny ◽

Multivariate Analyses ◽

Morphological Characters ◽

Leaf Length ◽

Species Differentiation ◽

New Classification ◽

Species Relationship ◽

Petal Length ◽

Leaf Arrangement ◽

Flower Diameter

The ornamentally important genus Adonis L. (Ranunculaceae) consists of about 30 species. In Iran, 8 species are recognized. The new classification system recognizes two subgenera, six sections, and six series for Adonis. The species differentiation within the genus is controversial due to morphological overlaps in different species and extensive morphological variation. Therefore, the aims of present study were: 1- Species delimitation within the Sect. Adonis based on morphological and molecular evidences and, 2- Studying the species relationship based on molecular phylogeny. Multivariate analyses of morphological and molecular (ITS and ISSR) data differentiated the Adonis species. PCA biplot analysis of morphological characters revealed morphological characters such as leaf arrangement; leaf length, flower diameter, petal length and width, calyx length, and pedicel length are of taxonomic implication in Adonis. Bayesian tree of ITS sequences revealed that the sect. Adonis is differentiated from the sect. Adonanthe. Based on ITS data, A. microcarpa showed close affinity to A. scrobiculata, while A. aestivalis, A. icrocarpa and A.dentata were close to each other.

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Bamboo Diversity in Indrokilo Botanical Garden, Central Java

Buletin Plasma Nutfah ◽

10.21082/blpn.v27n1.2021.p57-70 ◽

2021 ◽

Vol 27 (1) ◽

pp. 57

Author(s):

Baiq Farhatul Wahidah ◽

I Putu Gede P. Damayanto ◽

Sri Mulyani

Keyword(s):

Scientific Information ◽

Similarity Index ◽

Botanical Garden ◽

Morphological Characters ◽

Similarity Analysis ◽

Bamboo Species ◽

Similarity Coefficients ◽

Botanical Gardens ◽

Identification Keys ◽

Central Java

The efforts of Bamboo conservation have been conducted by botanical gardens in Indonesia, including the Indrokilo Botanical Garden, Central Java. Scientific information regarding the bamboo collection of the Indrokilo Botanical Garden, however, is not yet available. This study aimed to determine the diversity of the species, as well as, to provide a synopsis and the similarity analysis of the bamboo species in the Indrokilo Botanical Garden. Exploration and collection of bamboo specimens have been carried out by the Indrokilo Botanical Garden. Processing and identification of the specimens were carried out at Herbarium Bogoriense. A total of 27 morphological characters were used for similarity analysis using the UPGMA method with Nei & Li similarity coefficients. The data were analyzed descriptively. There are seven species of bamboo in the Indrokilo Botanical Garden: Bambusa glaucophylla, B. lako, B. multiplex, B. vulgaris, Dendrocalamus sp., Guadua cf. angustifolia, and Schizostachyum sp. A synopsis of the bamboo species and their identification keys to the species were provided. Based on the similarity analysis, bamboo in the Indrokilo Botanical Garden consists of five groups at a similarity index of 70%. The diversity of bamboo species in the Indrokilo Botanical Garden is relatively low compared to some botanical gardens in Indonesia.

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Spikemoss flora (Selaginella) in Mindanao Island, the Philippines: species composition and phenetic analysis of morphological variations

10.26757/pjsb.2018a12007 ◽

2018 ◽

Vol 12 (1) ◽

Keyword(s):

Species Richness ◽

New Species ◽

Species Composition ◽

Morphological Characteristics ◽

Morphological Characters ◽

The Philippines ◽

Phenetic Analysis ◽

Morphological Variations ◽

Diverse Species ◽

Phenetic Relationships

The genus Selaginella, commonly referred to as spikemosses, is an important component of the Philippines’ lycophytes diversity. Mountain ecosystems in Mindanao island hold diverse species of lycophytes, however the morpho-taxonomy diversity of this group is poorly documented there. The study aimed to determine Selaginella species richness, distribution and phenetic relationships based on morphological characteristics in Mindanao island. Specimens of Selaginella were collected in Mt. Apo, Kidapawan City; Mt. Hamiguitan, Davao Oriental; Mt. Kiamo, Mt. Kitanglad, Mt. Musuan and Mt. Limbawon in Bukidnon; Mt. Timpoong, Camiguin; and Mt. Malindang, Misamis Occidental. The 24 Selaginella species were collected and subjected to numerical phenetic analysis using the PC-ORD program. A total of 11 morphological characters and 44 character states were used in the analyses. Of the 24 species, seven are Philippine endemics, four are potential new species and two species are widely distributed in the areas studied. Results of the phenetic analysis showed four major clusters.

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Effects of Gamma Irradiation on Phenotypic Changes in Vanda Hybrid

Journal of Biodiversity and Biotechnology ◽

10.20961/jbb.v1i1.49298 ◽

2021 ◽

Vol 1 (1) ◽

pp. 1

Author(s):

Sri Hartati ◽

Prasetyo Prasetyo ◽

Endang Setia Muliawati

Keyword(s):

Gamma Irradiation ◽

Plant Height ◽

Gamma Ray ◽

Ornamental Plants ◽

Morphological Characteristics ◽

Morphological Characters ◽

Leaf Length ◽

Gamma Ray Irradiation ◽

Number Of Leaves ◽

Light Green

Vanda Orchid is one of the most popular ornamental plants. Orchids have slow growth. One way to overcome this weakness is by using gamma-ray irradiation. This research aimed to study the morphological characters of various radiation doses resulting from the crossing of the Vanda Orchid. This study was carried out by observing the characters of vegetative growth for each plant on 0 Gy, 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy doses of gamma irradiation treatments. The observed variables were the increase in plant height, leaf length, root length, number of roots, number of leaves, leaf width, and leaf color. The results showed that the dose of gamma-ray irradiation were able to increase plant height. 10 Gy and 20 Gy, gamma-ray irradiation doses, resulted in discolored yellowing of orchid leaves. The discoloration of orchid leaves to light green occurs at irradiation doses of 30 Gy, 40 Gy, and 50 Gy. The morphological characteristics of Vanda sp. hybrid results gamma irradiation are high at dose 10-40 Gy. Keywords: characteristics; morphological; orchid.

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Durian variety (Durio zibethinus L.) in Kota Bahagia District, South Aceh, Indonesia

JURNAL BIOLOGI TROPIS ◽

10.29303/jbt.v21i1.2361 ◽

2021 ◽

Vol 21 (1) ◽

pp. 42

Author(s):

Mardudi Mardudi ◽

Eka Selviyanti ◽

Adi Bejo Suwardi

Keyword(s):

Data Collection ◽

Southeast Asia ◽

Field Observation ◽

Similarity Index ◽

Morphological Characteristics ◽

Morphological Characters ◽

Tropical Fruit ◽

Association Coefficient ◽

Durio Zibethinus ◽

Index Value

Durian (Durio zibethinus L.) is one of tropical fruit grown in Southeast Asia and known as King of the fruit. Durian (D. zibethinus) has many varieties with distinctive of morphological characters. The study aim is to explore the diversity of durian varieties in Kota Bahagia sub-district, Aceh, Indonesia. This study was carried out in October-December 2020 in five villages, namely Jambo Keupok, Alur Duamas, Seneubok Alur Buloh, Seneubok Keranji and Beutong. Data collection on the morphological characteristics of durian varieties was carried out through direct field observation of morphology. The data were tabulated and the similarity index was calculated. A total of 18 varieties of durian were found in 5 villages in Kota Bahagia sub-district, i.e. Jantung, Semut, Nanas, Kunyit, Bintang, Tamago, Labu, Bingen, Patai, Jelatang, Jerat, Ampo, Toba, Lilin, Kondo, Limeng, Langsat, dan Gadang Tampok. The highest similarity index value was found in Limeng (Lm) and Patai (Pa) varieties with an association coefficient value of 63.04, while the lowest values were found in Nanas (Na) and Semut (Se) varieties and Kondo (Ko) and Nanas (Na) varieties with an association coefficient of 15.21.

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Phenetic analysis of Curcuma spp. in Yogyakarta, Indonesia based on morphological and anatomical characters

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d200832 ◽

2019 ◽

Vol 20 (8) ◽

Author(s):

Mulya Sungkawati ◽

L. HIDAYATI ◽

B.S. DARYONO ◽

Purnomo Purnomo

Keyword(s):

Economic Value ◽

Similarity Index ◽

Morphological Characteristics ◽

Principal Component ◽

Phenetic Analysis ◽

Anatomical Characteristics ◽

Morphological Variations ◽

Phenetic Relationships ◽

Anatomical Characters ◽

Group B

Abstract. Sungkawati M, Hidayati L, Daryono BS, Purnomo. 2019. Phenetic analysis of Curcuma spp. in Yogyakarta, Indonesia based on morphological and anatomical characters. Biodiversitas 20: 2340-2347. Curcuma spp., also known as ginger (Zingiberaceae), has economic value in traditional medicine. However, its many morphological variations cause difficulties in identification and classification. Therefore, observation of its morphological and anatomical characteristics, and of the phenetic relationships between Curcuma species, is important. This research aims to determine the specific characteristics of Curcuma spp. and to examine its phenetic relationships based on morphological and anatomical characteristics. The research was conducted in July 2018-February 2019 in the Bantul, Gunungkidul, Sleman and Karanganyar regions. Observation of the anatomical characteristics was conducted on the leaves and rhizomes and the data analyzed according to descriptive and quantitative/numerical methods. Clustering analysis with the Gower General Similarity Coefficient and Principal Component Analysis (PCA) was performed to determine the role of each character in groupings. The results of the research found seven species from 23 OTUs observed (C. aeruginosa, C. domestica, C. manga and C. xanthorrhiza, each with four OTUs; C. soloensis and C. zedoaria, with three OTUs each; and C. heyneana with one OTU) and showed that specific morphological characteristics were found in the flesh color rhizome, pseudostem color and midrib color. Specific anatomical characteristics were evident in the secretion cell color and the presence of trichomes on the leaves and rhizomes. The dendrogram shows a 0.70 phenon line consisting of two groups, group A (C. soloensis and C. domestica) fused in a 0.760 similarity index, and group B (C. aeruginosa, C. mangga, C. heyneana, C. soloensis, C. xanthorrhiza and C. zedoaria) fused in a 0.654 similarity index, which means that C. soloensis and C. domestica have a close phenetic relationship. The 0.80 phenon line consisted of five groups: C. domestica, C. soloensis, C. xanthorrhiza, C. zedoaria-C. mangga-C. heyneana and C. aeruginosa.

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Variation on morphology and spore characters of Dicranopteris and Sticherus (Gleicheniaceae) from Rokan Hulu District, Riau, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d221041 ◽

2021 ◽

Vol 22 (10) ◽

Author(s):

Afni Atika Marpaung ◽

Ratna Susandarini

Keyword(s):

Principal Component Analysis ◽

Cluster Analysis ◽

Principal Component ◽

Component Analysis ◽

Morphological Characters ◽

Morphological Variations ◽

Phenetic Relationships ◽

The Family ◽

Taxonomic Approach

Abstract. Marpaung AA, Susandarini R. 2021. Variation on morphology and spore characters of Dicranopteris and Sticherus (Gleicheniaceae) from Rokan Hulu District, Riau. Biodiversitas 22: 4475-4486. Dicranopteris and Sticherus are genera in the family Gleicheniaceae which have unique habits and distinctively specific growth with the presence of a bud on the forking branches. This study aims to reveal the species diversity of Gleicheniaceae members found in the forest area of Bukit Suligi Tandun, Rokan Hulu District, Riau and to document variations in their morphological characters and spores. Eleven collections were obtained from the exploration in the study area. The identification of plant specimens showed that these eleven taxa consisted of Dicranopteris curranii, Dicranopteris speciosa, seven varieties of Dicranopteris linearis, and two varieties of Sticherus truncatus. Seven varieties within D. linearis were D. linearis var. linearis, D. linearis var. alternans, D. linearis var. altissima, D. linearis var. demota, D. linearis var. inaequalis, D. linearis var. subspeciosa, and D. linearis var. tetraphylla. The only one species of Sticherus found was S. truncatus which consisted of two varieties, namely S. truncatus var. truncata and S. truncatus var. involuta. Morphological characters were observed from fertile specimens, while spore characters were observed from spore microscopic slides prepared using acetolysis method. There were 42 morphological characters and spores examined and used as the basis for determining the phenetic relationships using cluster analysis and principal component analysis. The results of cluster analysis showed the formation of two clusters that clearly separated the genus Sticherus from Dicranopteris. The three species of Dicranopteris were also clearly separated from each other in different sub-clusters. The result of principal component analysis indicated characters that contribute to the grouping of the eleven taxa and was able to explain the distinguishing characters in each group recognized from the cluster analysis. Overall, the results of this study contributed to documenting in detail the morphological variations in sporophyte and spore characters in eleven taxa of Dicranopetris and Sticherus, as well as proving the role of morphological and spore characters in the classification and defining phenetic relationships using numerical taxonomic approach.

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Readjustment of the Standard ASUDAS to Encompass Dental Morphological Variations in Plio-Pleistocene Hominins

Bulletins et Mémoires de la Société d anthropologie de Paris ◽

10.3166/bmsap-2018-0002 ◽

2018 ◽

Vol 30 (1-2) ◽

pp. 32-48

Author(s):

M. Louail ◽

S. Prat

Keyword(s):

Phylogenetic Relationships ◽

Homo Sapiens ◽

Initial Study ◽

Morphological Characters ◽

State University ◽

Arizona State University ◽

Dental Anthropology ◽

Morphological Variations ◽

Fossil Hominins ◽

Fossil Hominin

The standard ASUDAS scoring system (Arizona State University Dental Anthropology System) is used to assess dental morphological variations in modern humans. It is also frequently used to study, score, and compare morphological variations in fossil hominin taxa and to examine their phylogenetic relationships. However, using ASUDAS in studies of this type is under debate because it is based on modern Homo sapiens populations and does not appear to cover all variations observed in fossil Plio-Pleistocene homi- nins. Our observations and coding of 178 dentals casts of Plio-Pleistocene specimens based on ASUDAS and from the literature have confirmed the need to adapt the standard system to fossil hominins. In this initial study, we propose that the scoring procedures for some morphological characters need to be readjusted, while others could be standardized following the ASUDAS system.

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Thermo-Chemical Treatment for Carcass Disposal and the Application of Treated Carcass as Compost

Applied Sciences ◽

10.3390/app11010431 ◽

2021 ◽

Vol 11 (1) ◽

pp. 431

Author(s):

Jae-In Lee ◽

Eun-Ji Cho ◽

Fritz Ndumbe Lyonga ◽

Chang-Hee Lee ◽

Sue-Yun Hwang ◽

...

Keyword(s):

Chemical Treatment ◽

Dry Weight ◽

Heavy Metal Concentration ◽

Leaf Length ◽

Air Pollutant ◽

Germination Index ◽

Pollutant Emission ◽

Reinforced Plastic ◽

Korean Ministry ◽

Index Value

A mechanized thermo-chemical treatment system was developed to treat the undecomposed carcass and remediate livestock burial sites. Animal carcasses were thus processed via crushing, mixing, and treatment with quicklime treatment, heat treatment (200–500 °C), and mixing with sawdust. The machinery was applied to two sites where 16,000 chickens and 418 pigs had previously been buried in fiber-reinforced plastic storage bins. No dioxins were detected in the gas discharged during processing, and the concentration of total volatile organic compound, toluene, ethylbenzene, xylene, and styrene were 430.3, 139.0, 18.3, 21.4, and 10.4 μg/m3, respectively, which were below the air pollutant emission standards issued by the Korean Ministry of Environment. Korean standards stipulating the use of treated carcasses as compost (C, N, and P content, heavy metal concentration, Escherichia coli, and Salmonella) were met, but the germination index value was less than 70, not satisfying the criteria. Plant height, leaf length, leaf width, and dry weight of lettuce grown in soil amended with treated carcass product were significantly lower than those grown in low nutrient soil due to the poor germination index of the treated carcass. These results indicate that a composting process is required before the use of the treated carcass as a fertilizer. The addition of zeolite retarded the elution of ammonia from the carcasses and its efficiency was about 87.9%. It is expected that the mechanized thermo-chemical treatment process developed in this study could replace other technologies for the remediation of livestock burial sites.

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