scholarly journals EVALUATION OF ANTIMICROBIAL AND SYNERGISTIC ANTIMICROBIAL PROPERTIES OF PTEROCARPUS SANTALINUS

2017 ◽  
Vol 10 (11) ◽  
pp. 204 ◽  
Author(s):  
Savan Donga ◽  
Pooja Moteriya ◽  
Sumitra Chanda
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Polymyxin B ◽  
Bark Extract ◽  
Synergistic Activity ◽  
Agar Disc ◽  
Pterocarpus Santalinus ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay ◽  
Agar Disc Diffusion Assay

Objectives: The aim of the present study was to evaluate antibacterial and synergistic antimicrobial properties of leaf, stem and bark of Pterocarpus santalinus. Methods: The extraction was done by decoction method. The antimicrobial activity was done by agar well diffusion assay and the synergistic antimicrobial activity was done by agar disc diffusion assay.  Results: The synergistic activity was studied with plant extracts plus antibiotics viz. Ampicillin , Polymyxin-B,  Clotrimazole and  Fluconazole.  Conclusions: Amongst the three parts, the best antimicrobial activity was shown by bark extract. All the three parts showed synergistic antimicrobial activity with antibiotics but their level varied.  The results suggest that all the three parts phytochemicals that enhance the antimicrobial efficacy of the antibiotics against some microorganisms and hence can be developed as a new therapeutic weapon against infectious diseases.

scholarly journals ONE-POT SYNTHESIS OF 2,4,5-TRIARYLIMIDAZOLES FROM KETO-OXIMES: CHARACTERIZATION AND EVALUATION OF ANTIMICROBIAL ACTIVITY

2019 ◽  
pp. 263-270
Author(s):  
KRANTHI KUMAR T ◽  
SREENIVASULU R
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activities ◽  
Antimicrobial Properties ◽  
Antimycobacterial Activity ◽  
Diffusion Method ◽  
Current Investigation ◽  
One Pot ◽  
Fungal Strains ◽  
Aryl Aldehydes ◽  
Agar Disc

Background and Objective: Imidazole scaffold is pervasive in pharmaceuticals and it possesses diverse type of biological activities, especially triarylimidazole derivatives are biologically prominent molecules which inspired the current investigation. The objective of the work is to synthesize 15 novel 2,4,5-triarylimidazole derivatives and evaluate their antimicrobial and antimycobacterial activity against selected bacterial and fungal strains. Methods: The title compounds 2,4,5-triaryl-imidazole were synthesized from the corresponding aryl aldehydes and keto-oximes through the cyclization to N-hydroxyimidazoles and reduced thermally to the different imidazole derivatives. Agar disc diffusion method is employed for the antimicrobial and antimycobacterial studies. Results: Fifteen novel 2,4,5-triarylimidazoles were synthesized in adequate yields and characterization of the molecules was done by detailed spectral analysis using advanced analytical support. Results disclosed that all the synthesized compounds were exhibiting antimicrobial properties. Compounds 3h, 3g, 3b, and 3m were stated to possess potent antimicrobial properties in the given bacterial and fungal strains. Conclusion: The current investigation results support the antimicrobial and antimycobacterial activity of the synthesized 2,4,5-triarylimidazole derivatives. Further, research is necessary to explore the mechanism involved in the antimicrobial activity.

scholarly journals Antimicrobial Activity of Opuntia cochenillifera (L.) Mill Fruit and Cladode Extracts

2016 ◽  
Vol 3 ◽  
pp. 84-89
Author(s):  
Pooja Suryawanshi ◽  
G.M. Vidyasagar
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Maximum Activity ◽  
Methanolic Extracts ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

In the present study the antimicrobial activity of chloroform and methanolic extracts of Opuntiacochenillifera for both cladode and fruits was investigated. Methanolic extract was found to be an effective against the microbes namely, E. coli,Bacillus subtilis,Staphylococcus aureus, Pseudomonas aeruginosaCandidaalbicanC.glabrataC.haemuloniiC.Tropicalis. Maximum activity was observed against E. coli, B. Subtilisand,C.albican and C.glabrata at 40mg/ml. Agar well diffusion assay was used to determine minimum inhibitory concentration of all test microorganisms.

scholarly journals Antimicrobial activity of leaf extracts of Euphorbia paralias L. and Melilotus sulcatus Desf. against some pathogenic microorganisms

2021 ◽  
pp. 10-15
Author(s):  
Miloud M. Miloud ◽  
Najma A. Senussi
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Methanolic Extract ◽  
Bacterial Species ◽  
Fungal Species ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Bioactive Agents ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

The present study was aimed to investigate the antimicrobial potential of leaf extracts of Euphorbia paralias and Melilotus sulcatus against four bacterial species Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella sp. and two fungal species Asperigillus niger and Aspergillus flavus. The agar well diffusion assay was used to evaluate the antimicrobial activity. The effect of these extracts was most effective against the bacterial species compared to the fungal species at a used concentration (100 mg/ml). Methanolic extracts of selected plants displayed good antimicrobial activity against all tested microorganisms species, while, no activity for aqueous extracts against tested fungal species. Methanolic extracts were the most effective plant extracts against all tested bacterial species, with MIC and MBC reached 6.2 and 12.5 mg/ml, except Klebsiella sp. which was less sensitive to M. sulcatus methanolic extract and its MIC and MBC reached 12.5 and 25 mg/ml, respectively. These plant extracts which proved to be potentially effective can be used as bioactive agents to control microorganisms caused for diseases and they can be used naturally in the human and veterinary healthcare systems.

scholarly journals In vitro leaves and twigs antimicrobial properties of Psidium guajava L. (Myrtaceae)

2015 ◽  
Vol 61 (3) ◽  
pp. 93-104
Author(s):  
Basel Saleh ◽  
Lila Al-Halab ◽  
Ayman Al-Mariri
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Inhibitory Concentration ◽  
Activity Index ◽  
Antimicrobial Properties ◽  
Psidium Guajava ◽  
Hot Water ◽  
Antibacterial And Antifungal ◽  
Diffusion Assay

Summary The current study aimed to investigate antimicrobial activity of methanol, ethanol, acetone, ethyl acetate and hot water (leaves and twigs fractions) Psidium guajava L. crude extracts against three bacteria and two fungi pathogens. Antimicrobial activity expressed by disc-diffusion assay (zone of inhibitions - ZIs), activity index (AI) and minimum inhibitory concentration (MICs) that were measured as reported in many investigations. Based upon the estimated ZIs, AI and MICs values, hot water twigs <1 cm diameter extract was the most potent against all tested microorganisms. The MICs value ranged between 4 and 7.2 mg/ml for bacteria, while, it was between 14.5 and 37.3 mg/ml for fungi. Moreover, ethyl acetate had the lowest antimicrobial activity compared to the other tested solvents. From the results obtained herein, it could be concluded that P. guajava serve as antibacterial and antifungal agent.

In Vitro Antimicrobial Properties of Propolis against Caries-Associated Microorganisms

2015 ◽  
Vol 752-753 ◽  
pp. 387-390
Author(s):  
Boonyanit Thaweboon ◽  
Sroisiri Thaweboon ◽  
Rattiporn Kaypetch ◽  
Thaniya Muadcheingka
Keyword(s):  
Antimicrobial Activity ◽  
Molecular Mechanisms ◽  
Biological Activities ◽  
Oral Care ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Scientific Basis ◽  
Agar Disc ◽  
Zones Of Inhibition

Natural products have attracted much attention in recent years as a useful substance used in medicine and dental products. Propolis is a lipophilic resinous mixture collected from various plant sources by honeybees. It has been known for many biological activities including antimicrobial properties. Objective: To evaluate thein vitroantimicrobial activity of propolis against caries-associated microorganisms,Streptococcusmutans,LactobacilluscaseiandCandidaalbicans. Materials and methods: The antimicrobial activity was initially screened using agar disc diffusion and then the minimum lethal concentrations (MLCs) were determined using broth dilution technique. Chlorhexidine (0.2%) and dimethyl sulfoxide (5%) were used as positive and negative controls, respectively. Results: Propolis exhibits the zones of inhibition ranged from 15 to 18 mm forS.mutans, and from 10 to 12 mm forL.casei, but presented no activity againstC.albicans. The MLC againstS.mutanswas 2.86 mg/mL and those againstL.caseiwere 11.44 mg/mL and 12.87 mg/mL. Conclusion: Propolis shows a potent antimicrobial effect againstS.mutansandL.casei.This could provide a scientific basis to its application of usage in oral care products for the prevention of dental caries. However, further studies are necessary to evaluate other biological activities and molecular mechanisms of action as well as its cytotoxic effect.

scholarly journals DETECTION OF THE ANTIMICROBIAL ACTIVITY OF SILVER NANOPARTICULES BIOSYNTHESIZED BY STREPTOCOCCUS PYOGENES BACTERIA

2020 ◽  
Vol 51 (2) ◽  
pp. 500-507 ◽  
Author(s):  
Kadhum & Hussein
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Streptococcus Pyogenes ◽  
X Ray Diffraction ◽  
Pathogenic Microbes ◽  
Vitek 2 ◽  
Uv Visible ◽  
Agar Well Diffusion Assay ◽  
Moderate Range ◽  
Diffusion Assay

This study was aimed to biosynthesized silver nanoparticles by Streptococcus pyogenes bacteria and its antimicrobial activity against (S.aureus ,P. aeruginosa, E.coli, and C. albicans yeast) at different  concentrations (20, 40, 60 ,80 and 100)  μg/ml by agar  well  diffusion  assay. Fifty sample was collected from Wounds and burns, from Baghdad Teaching City Medicine Laboratories. Samples identified by culture, VITEK 2 Compact system ID-YST kit. The sensitivity of bacterial isolates to antibiotics ware tested and the microbes were more  sensitive,  resistant  and  moderate  range to antibiotics. Several techniques where used to characterize AgNPs: X-ray Diffraction (XRD), UV–Visible Spectroscopy(UV) and Scanning electron microscope (SEM).The results show that biosynthesized silver nanoparticles are more effective than bacterial supernatant on human pathogenic microbes.

scholarly journals Antimicrobial Activity of Endophytic Fungi Isolated from Swietenia macrophylla Leaves

2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Darah Ibrahim ◽  
Chong Chai Lee ◽  
Lim Sheh-Hong
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Leaf Age ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Swietenia Macrophylla ◽  
Stages Of Growth ◽  
Agar Plug ◽  
Fungal Assemblages ◽  
Diffusion Assay

The endophytic fungi isolated from leaves of Swietenia macrophylla of different ages were examined for antimicrobial activity. The agar plug diffusion assay was used for primary screening, followed by the disc diffusion method. A total of 461 filamentous endophytic fungi were isolated and cultured to examine their antimicrobial properties. In the primary screen, 315 isolates (68.3%) exhibited activity against at least one of the test pathogenic microorganisms. The percentage of isolates exhibiting antimicrobial activity increased with leaf age. Endophytic fungal assemblages, as well as those isolates exhibiting antimicrobial properties appeared to increase with leaf age. The main antimicrobial compounds were produced extracellularly by the endophytic fungi. The results suggest that healthy leaves at older stages of growth can be a potential source for the isolation of endophytic fungi with antimicrobial properties.

Mangostanaxanthone VII, a new cytotoxic xanthone from Garcinia mangostana

2018 ◽  
Vol 73 (5-6) ◽  
pp. 185-189 ◽  
Author(s):  
Sabrin R.M. Ibrahim ◽  
Gamal A. Mohamed ◽  
Mahmoud A. Elfaky ◽  
Mohamed F. Zayed ◽  
Amal A. El-Kholy ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mcf7 Cell ◽  
2D Nmr ◽  
Garcinia Mangostana ◽  
Spectral Techniques ◽  
Sulforhodamine B ◽  
Agar Disc ◽  
Diffusion Assay ◽  
Agar Disc Diffusion Assay ◽  
Mcf 7

Abstract Garcinia mangostana L. (the queen of fruits, mangosteen, family Guttiferae) is a wealthy source of xanthones. The CHCl3 soluble fraction of the air-dried pericarps of G. mangostana provided a new xanthone: mangostanaxanthone VII (5), along with four known xanthones: mangostanaxanthones I (1) and II (2), gartanin (3) and γ-mangostin (4). The structural verification of these metabolites was achieved by different spectral techniques, including UV, IR, 1D and 2D NMR and HRESIMS. The new metabolite was assessed for cytotoxic potential, using sulforhodamine B (SRB) assay towards the A549 and MCF-7 cancer cell lines. Moreover, its antimicrobial effects were evaluated against various bacterial and fungal strains, using agar disc diffusion assay. Mangostanaxanthone VII showed moderate cytotoxic activity against the A549 and MCF7 cell lines with IC50s 26.1 and 34.8 μM, respectively, compared with doxorubicin (0.74 and 0.41 μM, respectively).

scholarly journals Antibacterial potential of lactobacilli isolated from a lamb

2011 ◽  
Vol 56 (No. 7) ◽  
pp. 319-324 ◽  
Author(s):  
A. Bilkova ◽  
H. Kinova Sepova ◽  
M. Bukovsky ◽  
L. Bezakova
Keyword(s):  
Inhibitory Activity ◽  
Pathogenic Bacteria ◽  
Lactobacillus Reuteri ◽  
Lactobacillus Rhamnosus ◽  
Antimicrobial Properties ◽  
Residual Activity ◽  
Line Method ◽  
Agar Well Diffusion Assay ◽  
Lactobacillus Mucosae ◽  
Diffusion Assay

The antimicrobial properties of three potential probiotic strains of lactobacilli isolated from a lamb (Lactobacillus murinus C, Lactobacillus mucosae D and Lactobacillus reuteri E) were studied using the streak line method and the agar well diffusion assay. The probiotic lactobacilli strains Lactobacillus rhamnosus ATCC 53103, Lactobacillus reuteri ATCC 55730, Lactobacillus reuteri ATCC 55845 and Lactobacillus plantarum DSM 9843 were used for comparison. Using the streak line method the inhibitory activity of lactobacilli products towards ten Gram-positive and Gram-negative potential pathogenic bacteria under different cultivation conditions (anaeorobic or microaerobic preincubation of lactobacilli for 24 h or 48 h) was tested. The strongest inhibitory activity was demonstrated by the Lactobacillus reuteri E strain. The most sensitive strains to the antimicrobial activity of lactobacilli were Yersinia enterocolitica clinical isolate (19.9 &plusmn; 6.8 mm) and Listeria monocytogenes ATCC 51774 (17.7 &plusmn; 6.0 mm) after microaerobic and anaerobic preincubation, respectively. Generally, microaerobic conditions and longer preincubation of lactobacilli resulted in stronger inhibition of target bacteria. The inhibitory activity of lactobacilli towards selected lactobacilli strains was also tested. Only low inhibition of growth was observed. In the agar well diffusion assay the inhibitory effect of natural and modified lactobacilli culture cell-free supernatants, obtained from MRS broth cultures, on Staphylococcus aureus ATCC 6538 growth was determined. Supernatants were modified by heat (10 min/60 &deg;C; 60 min/100 &deg;C) and protease treatment and neutralization of pH. Neutralization elicited the most significant impact on the activity of supernatants and resulted in total loss of activity. After all other modifications supernatants retained some residual activity. The highest inhibitory activity was observed for the cell-free supernatant produced by Lactobacillus mucosae D.

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