scholarly journals EFFECT OF SUB-CHRONIC EXPOSURE TO CHROMIUM ON HAEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF MALE ALBINO RAT

2017 ◽  
Vol 10 (5) ◽  
pp. 345 ◽  
Author(s):  
Jahnabi Saha ◽  
Soma Choudhuri ◽  
Dipayan Choudhuri
Keyword(s):  
Toxic Effect ◽  
Blood Cells ◽  
Chronic Exposure ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Transition Element ◽  
Albino Rats ◽  
Albumin Concentration ◽  
Chromium Vi

Objectives :  Exposure to chromium, the transition element found in many compounds of earth’s crust, leads to various health hazards including cancer, dermatitis, damage of liver  and kidneys and reproductive toxicity in both male and female. Toxic  effect of chronic exposure to chromium at low environmentally relevant dose is recently recognized and less studied. In the present study, we evaluated the effect of sub-chronic exposure to low doses of chromium VI (K2Cr2O7) on various haematological and biochemical parameters in male albino rats.Methods : Male albino rats (160±10 gm) received 1/25, 1/50 and 1/100 of reported oral LD50 dose of chromium VI (K2Cr2O7) for 90 consecutive days. After treatment, the blood was collected for analysis of various haematological and biochemical parameters.Results :  Exposure to chromium VI at 1/25 LD50 dose caused decrease in body weight gain and reduction in the weight of the reproductive organs along with increase in the weight of liver and kidney. Numbers of red blood cells, white blood cells, platelets and haemoglobin concentration reduced with increase in clotting time. The total sperm count and motility was also reduced in exposed rats. There was decrease in plasma protein and albumin concentration, with increase in plasma glucose and cholesterol level. The activity of enzyme alanine aminotransferase and enzyme aspertate aminotransferase was stimulated in treated rats.Conclusion : The results of the present study revealed that chronic low dose  exposure to chromium VI resulted in a toxic effect in experimental male albino rats.  Keywords: Chromium VI (K2Cr2O7), Haematological parameters, Biochemical parameters.  

scholarly journals Haematological and Serum Biochemical Profile of Growing Yankasa Ram Lambs Fed Diets Containing Graded Levels of Sesame Residue

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Y. Garba ◽  
E. A. Adeola
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Serum Biochemical Parameters ◽  
Corpuscular Haemoglobin ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Mean Corpuscular Haemoglobin ◽  
Ram Lambs

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.

scholarly journals Effect of aspartame on biochemical and oxidative stress parameters in rat blood

2015 ◽  
Vol 67 (2) ◽  
pp. 535-545 ◽  
Author(s):  
Marko Prokic ◽  
Milica Paunovic ◽  
Milos Matic ◽  
Natasa Djordjevic ◽  
Branka Ognjanovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Lipid Peroxides ◽  
Hdl Cholesterol ◽  
Albino Rats ◽  
Total Body Mass ◽  
Antioxidative Status

Aspartame (ASP) is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks) in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased in the ASP-treated rats. Treatment with ASP caused an increase in the concentrations of glucose, cholesterol, LDL-cholesterol, and in the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH), as well as a decrease in the levels of HDL-cholesterol in the serum. A significant decline in the number of white blood cells (WBC) was observed after ASP uptake. Based on the results we conclude that ASP induces oxidative stress, observed as an alteration of the glutathione redox status, which leads to increased concentrations of nitric oxide (NO) and lipid peroxides (LPO) in the red blood cells. Changes in biochemical parameters, lipid metabolism, as well as changes in the levels of oxidative stress markers and the appearance of signs of liver damage indicate that chronic use of ASP can lead to the development of hyperglycemia, hypercholesterolemia and associated diseases.

scholarly journals Effects of diet supplementation with different level of Celmanax® in rainbow trout (Oncorhyncus mykiss)

2019 ◽  
Vol 70 (1) ◽  
pp. 1347
Author(s):  
A. KHODADADI ◽  
A. HAGHIGHI ◽  
H. MALEKINEJADH ◽  
A. TUKMECHI ◽  
M. AFSHARNASAB
Keyword(s):  
Rainbow Trout ◽  
Neutrophil Count ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Serum Biochemical Parameters ◽  
Control Groups ◽  
Serum Biochemical ◽  
And Control

The aim of this study was to evaluate the effect of a prebiotic (Celmanax®) containing yeast cell wall with mannan oligosaccharides on the haematological and serum biochemical parameters in rainbow trout. Three levels of prebiotic (0, 0.1, 0.5 and 1 %) were mixed into pellets. Fish (19.08±1.45 g) were fed a supplemented commercial diet for 60 days. Blood samples were colected from the onset and on days 30 and 60 of the trial to measure the haematological and serum biochemical parameters in rainbow trout. The results showed significant differences in haemoglobin, mean corpuscular volume,mean corpuscular haemoglobin concentration, white blood cells and neutrophil count between control and all test groups (p<0.05). The highest and the lowest white blood cells and neutrophil count (on day 60) were observed in the 0.1 %, prebiotic-received and control groups, respectively. Also, the result showed significant differences in Alkaline phosphatase enzymes, serum glutamic oxaloacetic transaminase, Serum glutamic-pyruvic transaminase, between the test and control groups(p<0.05) while non-significant elevation of blood urea nitrogen, creatinine and total protein levels was found in the Celmanax®-received groups (p>0.05). These results suggest that the Celmanax® supplementation enhances white blood cells and neutrophil count, and changes some biochemical parameters in rainbow trout.

scholarly journals The Effect of Adding Protected Amino Acids in the Rumen (Methionine and Lysine and their Mixtures) on Some Physiological Characteristics of Male Arabi Lambs

2021 ◽  
Vol 13 (2) ◽  
pp. 16-23
Author(s):  
Yahya Harbi Abdul-Noor ◽  
Amad Falah Hassan
Keyword(s):  
Amino Acids ◽  
Growth Hormone ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Growth Hormones ◽  
Average Weight ◽  
Albumin Concentration ◽  
Treatment Control ◽  
Group 4

This study was conducted at the animal field of the Agriculture College / University of Basra / Karma Ali site for the period from 7/10/2019 to 1/5/2020 to investigation the effect of adding protected amino acids (methionine and lysine and their mixtures) on some physiological trails of male Arabi lambs. Sixteen male lambs with an average weight of (27.95) kg and an age of 5-6 months were selected, and randomly divided into four group (4 animals per group). The results indicated that there were no significant differences in the concentration of hemoglobin, packed cell volume, the number of red and white blood cells, cholesterol, triglycerides and the activity of the liver enzyme (ALT and AST) between the different treatments, while there was a significant (p <0.05) increase in the total protein and albumin concentration in the fourth treatment compared with the first treatment (control), while, there was a significant decrease (p <0.05) in the urea concentration for the fourth, third and second treatments compared with the first treatment. The concentrations of glucose, thyroxine and growth hormone increased significantly (p <0.05) in the fourth and second treatment compared with the first treatment. However, it can be concluded from the study that feeding the male lambs with 5g methionine or mixing it with lysine by 5g raises the concentration of glucose, growth hormones and thyroxine in the blood. Also, the levels of methionine and lysine used did not change the concentration of the studied biochemical parameters

scholarly journals Effect of salinity changes on growth, survival and biochemical parameters of freshwater fish Gibelion catla (Hamilton, 1822)

2021 ◽  
Vol 42 (6) ◽  
pp. 1519-1525
Author(s):  
S.K. Ahirwal ◽  
◽  
P.C. Das ◽  
K. Sarma ◽  
T. Kumar ◽  
...  
Keyword(s):  
Growth Rate ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Experimental Period ◽  
Quality Parameters ◽  
Control Fish ◽  
Feed Conversion ◽  
Nitrite Nitrate

Aim: The present investigation was conducted to access the effect of salinity stress on growth performance, survival and biochemical parameters of Gibelion catla under different salinity conditions with an idea to assess the potentiality of this species in inland saline affected areas. Methodology: A 30-day-experiment was conducted in 1000 l (n=10) FRP tanks to study the effect of different salinity levels on the growth and biochemical parameters of catla species. A total of 120 fish (10.4 g) was randomly distributed into four treatments (0, 3, 6 and 9 ppt) with three replicates. The water quality parameters such as pH, temperature, nitrite, nitrate, ammonia, alkalinity and dissolved oxygen were recorded for each treatment on weekly basis, whereas fish sampling was carried out at 0th and 30th day of the experimental period to ascertain survival, weight gain, specific growth rate and feed conversion ratio of the fishes from each treatment. Results: The highest growth rate was found in control subsequently in 3 and 6 ppt and the survival rate was 96.67, 83.33, 76.67 and 0% against 0, 3, 6 and 9 ppt, respectively. The red blood cells (3.65×106 μl) and haemoglobin concentration, (8.17 gm dl-1) were also higher in control fish, followed by 3 and 6 ppt. However, white blood cells (24.40×106 μl) and glucose level (123.23 mg dl-1) were higher at 6 ppt, compared to control. Plasma protein level of fish at control (0 ppt salinity) was significantly higher than those of fish exposed to 3 and 6 ppt salinities. Interpretation: The present investigation revealed that an increase in salinity level had a significant impact on the growth and physiology of Gibelion catla. However, this species can be reared in low saline areas for some time which will not only help in the utilization of salt affected areas but will also help in the generation of employment and income.

scholarly journals Haematological and serum biochemical indices of broiler chickens fed sorghum SK- 5912 ( , L. Moench) variety as a replacement for maize

2020 ◽  
Vol 45 (3) ◽  
Author(s):  
O.A. Lakurbe ◽  
U.D. Doma ◽  
K.M. Bello ◽  
M. Abubakar
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
Broiler Chickens ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Blood Analysis ◽  
Serum Biochemical Parameters ◽  
Feeding Trial ◽  
Serum Biochemical ◽  
Biochemical Indices

An eight-week feeding trial was carried out with three hundred unsexed day-old (Marshall) broilers to investigate the effect of sorghumSK-5912 variety as replacement for maize on the haematological and serum biochemical parameters of broiler chickens. Five experimental diets were formulated with SK-5912 replacing maize at 0, 25, 50, 75 and 100% in the diet and labelled as diets 1 (control), 2, 3, 4 and 5, respectively for both starter (23% CP) and finisher (20% CP) phases. The day-old chicks were randomly allotted to the dietary treatments with four replications each and 15 birds per replicate in a completely randomized design (CRD). Feed and water were supplied ad libitum and the experiment lasted eight weeks. At the end of the feeding trial eight birds per treatment were randomly selected and blood samples collected for haematological and serum biochemical analyses. Results from blood analysis showed no treatment effect on Packed Cell Volume (PCV), White Blood Cells (WBC), Red Blood Cells (RBC) counts, haemoglobin concentration (Hb) and corpuscular values. Similarly, there were no significant treatment effects among all the groups for serum biochemical indices. It can therefore be concluded that Sorghum SK-5912 can replace maize in the diets of broiler chickens without adverse effects on the haematological and serum biochemical parameters of the birds.

Hazardous Effects of HighVoltage on Albino Rats and Role of Rosmarinus Officinalis

2021 ◽  
Author(s):  
Sallm Tony ◽  
Sallam K. Tony ◽  
Hamadi A. Ismail ◽  
Fawzy S. Hatour ◽  
Magda E. Mahmoud
Keyword(s):  
Electromagnetic Field ◽  
Blood Cells ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Rosmarinus Officinalis ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Field Exposure ◽  
Blood Indices ◽  
The Impact

Abstract Electromagnetic fields (EMFs) are common in our everyday lives. They have many origins and severe effects on individuals and environments where they inflict a great deal of health and psychological harm. The current study investigated the impact of high voltage (H.V.) EMF 5.4 k / v for 2, 4 h per day with a frequency equal to 50 Hz on body weight(b.wt), blood indices, and certain liver enzymes of albino rats after 25 days of exposure to the electromagnetic field. This work focuses on the therapeutic action of methanol extract of Rosmarinus officinalis (R. officinalis) leaves at a dose (5 mg/kg b. wt) against harmful EMF-induced effects. The findings showed that electromagnetic field exposure induced a substantial decrease in red blood cells (RBC), haemoglobin concentration (Hb), and catalase activity (CAT). Although white blood cells (WBCs), aspartate aminotransferase (AST), alanine aminotransferase (ALT), Total Bilirubin, Urea, Creatinine, Uric Acid, and Malondialdehyde (MDA) levels have increased significantly under EMF treatment. Treatment with R.officinalis showed attenuation in these parameters that were induced in rats exposed to H.V. These findings were followed by the histopathological analysis of the liver in the observations. Finally, we conclude that R.officinalis leaves extract offered substantial protection against H.V induced liver damage and can be applied in drug production.

scholarly journals Influence of heavy metals on physiological and biochemical parameters of Pseudorasbora parva (Cypriniformes, Cyprinidae)

2021 ◽  
Vol 12 (4) ◽  
pp. 745-752
Author(s):  
O. M. Marenkov ◽  
O. O. Izhboldina ◽  
M. M. Nazarenko ◽  
R. V. Mylostyvyi ◽  
O. M. Khramkova ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Toxic Effect ◽  
Dehydrogenase Activity ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Pseudorasbora Parva ◽  
Lactate Dehydrogenase Activity ◽  
The Impact ◽  
Physiological And Biochemical

Anthropogenic load on aquatic ecosystems leads to increased inputs of heavy metals, which can have a toxic effect on aquatic organisms. Some of the most appropriate objects for research are short-cycle fish species. This article considers the results of studies on the adaptive reactions of the stone moroko Pseudorasbora parva (Temminck et Schlegel, 1846) to Mn, Pb, Ni heavy metal ions, which exceeded the reference values in the reservoir by 1.7, 1.5 and 2.0 times, respectively. Changes in morphological parameters of the blood and histocytological pattern of the hepatopancreas of the experimental species under the influence of toxicants, as well as changes in biochemical parameters, were determined. It was noted that the influence of Mn caused pathological changes in the form of poikilocytosis. The morphometric parameters of erythrocytes (the cell area and the nuclear area) reached 67.48 ± 0.67 and 13.97 ± 0.22 µm2 respectively (4.0% and 13.8% less compared to the control). The influence of Ni resulted in an increased number of leukocytes and immature forms of erythrocytes. The area of erythrocytes was 0.9% smaller than that of the control group, and the area of the nucleus was 4.5% smaller than in the control. The effect of Pb as well as Mn consisted in poikilocytosis. The area of red blood cells and nuclei was smaller by 6.5% and 8.3%, respectively, compared to the control. The percentage of white blood cells in fish exposed to Ni and Pb tended to increase. In the experiment with nickel, the percentage of white blood cells was 10.2% of the number of formed elements; in the experiment with lead – 11.3%; with manganese – 6.1%, while in the control, the number of white blood cells compared to the total number of formed elements of fish was only 1.2%. Cytometric studies have revealed that there are significant differences in the shape, size, and location of hepatocytes in different experimental fish. The structural components of the liver for histological specimens were stained differentially with different intensities and different colours. The hepatocytes on histological specimens of the liver of the stone moroko exposed to Ni and Mn ions did not have clearly defined boundaries, there was a large number of destroyed cells, which indicates the toxic effect of these heavy metals. The hepatocytes exposed to Pb had the largest area of cells and nuclei and the highest nuclear-cytoplasmic ratio is typical for hepatocytes under the influence of Mn. The nuclei had the largest size and occupied 12.7% of the internal contents of the cells. It is shown that under the impact of the studied concentrations of Mn, Ni and Pb, lactate dehydrogenase activity increased by 1.22, 1.14 and 1.48 times compared to the control, respectively. In contrast, there was a 3.27-fold decrease in succinate dehydrogenase activity under the impact of Mn. Besides that, the activity of the enzyme decreased by 1.48 and 1.68 times under the action of Ni and Pb. Subsequently, we found an increase in the activity of alkaline phosphatase in muscle tissue by 3.25–3.94 times under the influence of the studied toxicants. Muscle protein levels under the impact of Mn were 1.14 times lower than in the control, the most distinct decrease in protein was found under the impact of Ni (1.53 times). The obtained data of physiological and biochemical reactions of the stone moroko to the influence of heavy metals provide an opportunity to predict changes in the species composition of fish fauna under conditions of excessive toxic pollution of ecosystems.

ALLIUM SATIVUM ESSENTIAL OIL (ASEO);

2017 ◽  
Vol 24 (04) ◽  
pp. 612-616
Author(s):  
Faisal Irshad ◽  
Hina Mawani ◽  
Sana Naz
Keyword(s):  
Essential Oil ◽  
Blood Cell ◽  
Blood Cells ◽  
Allium Sativum ◽  
White Blood Cells ◽  
Albino Rats ◽  
Serum Triglycerides ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Animal House

Objectives: To determine the effects of Allium sativum essential oil (ASEO)phytotherapy on serum triglycerides, total cholesterol, HDLc, LDLc and blood cell counts inalbino rat model. Study design: Experimental study. Setting and Duration: Animal House,Sindh Agriculture University and Isra University Hyderabad from May 2014 to January 2015.Materials and Methods: 60 albino rats were divided into four groups. Controls were givenPlacebo. Experimental rat groups were given ASEO 100 mg/kg, 200 mg/kg and 300 mg/kgorally for 30 days. Cardiac puncture was performed for blood sampling. Research variableswere analyzed on Statistix 10.0 (USA). Results: Blood lipids showed significant reduction invarious blood lipid fractions. Serum LDLc exhibited with a concomitant rise in serum HDLc (p=0.0001) in high ASEO treated rats. Red blood cells, white blood cells and platelet showedsignificant improvement ASEO fed rats (p=0.001). Conclusion: Allium sativum essential oil(ASEO) phytotherapy showed a rise in HDLc and a reduction in LDLc, triglycerides and totalcholesterol with improvement in red blood cell counts.

scholarly journals STAT3 methylation in white blood cells as a novel sensitive biomarker for the toxic effect of low-dose benzene exposure

2016 ◽  
Vol 5 (3) ◽  
pp. 800-807 ◽  
Author(s):  
Di Liu ◽  
Yujiao Chen ◽  
Pengling Sun ◽  
Wenlin Bai ◽  
Ai Gao
Keyword(s):  
Dna Methylation ◽  
Toxic Effect ◽  
Health Effects ◽  
Blood Cells ◽  
Low Dose ◽  
White Blood Cells ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Benzene Exposure

A cross-sectional study was conducted in a sample of 571 workers to explore the toxic effect and early sensitive biomarker of the health effects of low-dose benzene exposure (LDBE), as well as the correlation between DNA methylation and the toxic effect of LDBE.

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