EFFECTS OF ETHANOL CINCAU (CYCLEA BARBATA MIERS) LEAVES EXTRACT ON MACROPHAGE MIGRATION ON BURN WOUND

Objective: Macrophages are essential cells in burn wound healing. The primary function of macrophages is phagocytosis of microbes and necrosis tissue. Migration of macrophages into the wound area is affected by interleukin-1 (IL-1). Ethanol extract of Cincau or green grass jelly leaves or Cyclea barbata Miers contains pectin, flavonoids, and alkaloids. These compounds influence the inflammatory process. The purpose of this study was to reveal the effect of ethanol Cincau leaves extract on the expression of IL-1 and the number of macrophages in second degree burn wounds tissue. Methods: This study used 30 rats divided into six groups based on different treatments: NaCl 0.9% (K), silver sulfadiazine/(SSD) (P1), hydrogels (P2), Cincau extract 40% (P3), Cincau extract 50% (P4), and Cincau extract 60% (P5). Treatment was conducted over 4 days. On the 5th day, burn wounds tissues were taken, then immunohistochemistry staining was applied. Results: One-way ANOVA test on the expression of IL-1 showed a difference among groups (p<0.05) and on the number of macrophages (p<0.05). Post hoc tests on the expression of IL-1 showed Cincau extracts 40% and 50% treated-groups significantly differ with the NaCl 0.9% group but not with hydrogel treated group. The similar result showed Cincau extracts 40% and 50% treated-groups significantly differ from the control group but not with SSD and hydrogel groups. Conclusions: It can be concluded that ethanol extract of Cincau leaves can decrease IL-1 expression and increase the number of macrophages which have an important role in inflammation resolution of burn wounds.

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Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

10.1101/436444 ◽

2018 ◽

Author(s):

Yasuhiko Kaita ◽

Takehiko Tarui ◽

Hideaki Yoshino ◽

Takeaki Matsuda ◽

Yoshihiro Yamaguchi ◽

...

Keyword(s):

Wound Healing ◽

Therapeutic Effect ◽

Healing Process ◽

Control Group ◽

Type I ◽

Burn Wound ◽

Burn Wounds ◽

Burn Wound Healing ◽

Significant Difference ◽

Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

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Comparison Profile of Nutrition Status in Stage V Chronic Kidney Disease Patients Hemodialyzing Using New and Reuse Dialyzer

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v5i2.9613 ◽

2019 ◽

Vol 5 (2) ◽

pp. 17

Author(s):

Yuli Hermansyah ◽

Ika Aulia Kurniasari ◽

Heni Fatmawati

Keyword(s):

Oxidative Stress ◽

Wound Healing ◽

Antioxidant Properties ◽

Negative Control ◽

Silver Sulfadiazine ◽

Control Group ◽

Burn Wound ◽

Burn Wound Healing ◽

Degree Burn ◽

Significant Difference

Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine

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Evaluation of the Healing Effects of Hypericum perforatum and Curcumin on Burn Wounds in Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/6462956 ◽

2020 ◽

Vol 2020 ◽

pp. 1-5

Author(s):

Nevra Seyhan

Keyword(s):

Wound Healing ◽

Hypericum Perforatum ◽

Control Group ◽

Sprague Dawley ◽

Burn Wound ◽

Burn Wounds ◽

Burn Wound Healing ◽

Significant Difference ◽

Group A ◽

Second Degree

Background. For centuries, medicinal plants have been extensively used in wound healing of burn injuries. The aim of this study is to analyze comparatively the effects of curcumin and Hypericum perforatum (HP) on second-degree burn wounds in rats. Materials and Methods. This experimental study was conducted on 24 male Sprague-Dawley rats with second-degree burns. The animals were randomly divided into three groups. The burns were treated with curcumin (Group B) and Hypericum perforatum (Group C) with daily application. Group A was considered as the control group and received no medication. Histological parameters, reepithelization, granulation tissue formation, inflammation, and angiogenesis were assessed after the scar biopsy at the end of research. Results. All histological parameters of the control group showed statistically significant difference than the other groups (p<0.05). There was no statistically significant difference between Groups B and C in terms of reepithelization and inflammation (p=0.351, p=0.067). In the curcumin group compared with the HP group, a significant difference was observed for two parameters of fibrosis and angiogenesis (p<0.05). Conclusion. Both curcumin and Hypericum perforatum oil are effective in burn wound healing. Our findings showed a better quality of healing in curcumin-treated rats.

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Wound Healing Effect of Nonthermal Atmospheric Pressure Plasma Jet on a Rat Burn Wound Model: A Preliminary Study

Journal of Burn Care & Research ◽

10.1093/jbcr/irz120 ◽

2019 ◽

Vol 40 (6) ◽

pp. 923-929 ◽

Cited By ~ 2

Author(s):

Yoonje Lee ◽

Sanjaya Ricky ◽

Tae Ho Lim ◽

Ki-Seok Jang ◽

Hongjung Kim ◽

...

Keyword(s):

Wound Healing ◽

Atmospheric Pressure ◽

Atmospheric Pressure Plasma ◽

Plasma Jet ◽

Treated Group ◽

Control Group ◽

Atmospheric Pressure Plasma Jet ◽

Plasma Medicine ◽

Burn Wound ◽

Burn Wounds

Abstract Worldwide, an estimated 6 million patients seek medical attention for burns annually. Various treatment methods and materials have been investigated and developed to enhance burn wound healing. Recently, a new technology, plasma medicine, has emerged to offer new solutions in wound care. As the development of plasma medicine has shown benefit in wound healing, we aimed to assess the effects of plasma medicine on burn wounds. To investigate the effectiveness of a nonthermal atmospheric pressure plasma jet (NAPPJ) for burn wound treatment on a brass comb burn wound rat model. Burn wounds were made by applying a preheated brass comb (100°C) for 2 minutes, which resulted in four full-thickness burn wounds separated by three interspaces. Interspaces were exposed to NAPPJ treatment for 2 minutes and morphological changes and neutrophil infiltration were monitored at 0, 4, and 7 days post-wounding. The percentage of necrotic interspace was higher in the control group than in the plasma-treated group (51.8 ± 20.5% vs 31.5 ± 19.0%, P < .001). Moreover, the exposure of interspace to NAPPJ greatly reduced the number of infiltrating neutrophils. In addition, the percentage of interspace that underwent full-thickness necrosis in the plasma-treated group was smaller than that in the control group (28% vs 67%). NAPPJ exposure on interspaces has a positive effect on burn wounds leading to wound healing by reducing burn injury progression.

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bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway

Scientific Reports ◽

10.1038/s41598-021-82888-9 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Srijita Chakrabarti ◽

Bhaskar Mazumder ◽

Jadab Rajkonwar ◽

Manash Pratim Pathak ◽

Pompy Patowary ◽

...

Keyword(s):

Growth Factor ◽

Delivery System ◽

Wound Closure ◽

Collagen Matrix ◽

Fibroblast Cell ◽

Control Group ◽

Burn Wound ◽

Burn Wound Healing ◽

Degree Burn ◽

And Control

AbstractBurn injuries are most challenging to manage since it causes loss of the integrity of large portions of the skin leading to major disability or even death. Over the years, hydrogels are considered as a significant delivery system for wound treatment because of several advantages over other conventional formulations. We hypothesized that the bFGF-collagen-AgSD incorporated hydrogel formulation can accelerate the rate of burn healing in animal model and would promote fibroblast cell proliferation. Neovascularization and re-epithelialization is a hall mark of burn wound healing. In the present study, histopathological investigation and scanning electron microscopy of skin tissue of Wistar rats showed almost complete epithelialisation after 16 days in the treatment group. The developed hydrogel showed significantly accelerated wound closure compared with a standard and control group. The faster wound closure resulted from increased re-epithelialization and granulation tissue formation because of the presence of collagen and growth factor. Expressions of proteins such as TrkA, p- TrkA, ERK1/2, p-ERK1/2, NF-kβ, and p-NF-kβ involved in nerve growth factor (NGF) signalling pathway were analysed by western blot. All the findings obtained from this study indicated that the hydrogel can be considered as a promising delivery system against second degree burn by faster healing.

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Topical treatment of standardised burns with herbal remedies in model rats

Bosnian Journal of Basic Medical Sciences ◽

10.17305/bjbms.2005.3233 ◽

2005 ◽

Vol 5 (4) ◽

pp. 50-57 ◽

Cited By ~ 1

Author(s):

Fahir Bečić ◽

Nedžad Mulabegović ◽

Zakira Mornjaković ◽

Elvedina Kapić ◽

Senad Prašović ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Silver Sulfadiazine ◽

Herbal Remedies ◽

Control Group ◽

Therapeutic Effects ◽

Skin Area ◽

Burn Wound ◽

Burn Wounds ◽

Burn Wound Healing

Experimental studies of burns require the use of different animal models. The aim of this work was to establish experimental model of thermal injuries and to evaluate the effects of topical agents on healing of the burn wounds. Forty female Wistar rats were randomly classified in 4 groups and isolated for 2 weeks before the onset of experiment. Animals were primarily anaesthetized with pentobarbital-sodium and then shaved (skin area of their back with diameters 5 cm x 5 cm). A round metal stamp with contact area of 5 cm2 and total weight of 100 g was heated up to 80°C and then applied without additional pressure on the depilated skin of the back for 14 seconds. This procedure produced a standardized burn wound. Induced burn wounds were immediately drowned in the 4°C-water for 3 s in order to maintain microcirculation. After the inducement of thermal injures, all rats were treated with 1% silver sulfadiazine cream, herbal topical preparations or were not treated at all. Burn wounds were treated twice a day until the healing completion. The result of treatment application was a significant reduction of burn wound diameters. Herbal topical preparations expressed positive therapeutic effects on the parameters of burn wounds. The efficiency of silver sulfadiazine cream in burn wound healing was significantly more expressed in comparison to healing process in control group of animals (p<0,001). We conclude that herbal topical preparations efficiently caused the completion of burn wound healing process without scar formation.

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The Effectiveness of Edamame Seed (Glycine max L. Merril) Ethanolic Extract to Fibroblast Count on Second Degree Burn Wound Healing

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v5i3.6831 ◽

2019 ◽

Vol 5 (3) ◽

pp. 154

Author(s):

Arifah Nur Hasanah ◽

Ika Rahmawati Sutejo ◽

Enny Suswati

Keyword(s):

Wound Healing ◽

Antioxidant Properties ◽

Ethanolic Extract ◽

Negative Control ◽

Control Group ◽

Burn Wound ◽

Burn Wound Healing ◽

Degree Burn ◽

Significant Difference ◽

Second Degree

Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The study was conducted at University of Jember during November until December 2017. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. The number of fibroblast of each picture was processed using Image-J software. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine

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Burn wound healing effects of the root hydroethanolic extract of Cochlospermum planchonii in mice

International Journal of Biological and Chemical Sciences ◽

10.4314/ijbcs.v14i9.24 ◽

2021 ◽

Vol 14 (9) ◽

pp. 3275-3284

Author(s):

Kossi Metowogo ◽

Nakpane Fankibe ◽

Yendubé T. Kantati ◽

Kodjo Adi ◽

Tchin Darré ◽

...

Keyword(s):

Group Iv ◽

Control Group ◽

Burn Wound ◽

Burn Wounds ◽

Group Iii ◽

Burn Wound Healing ◽

Group I ◽

Group Ii ◽

Imagej Software ◽

Hydroethanolic Extract

Cochlospermum planchonii is a plant traditionally used in Togo to treat burn wounds. The present study aimed at evaluating the effect of the root hydroethanolic extract of Cochlospermum planchonii on the skin burn wounds. Burns were induced in ICR mice by applying on the skin of the dorsal region with a metal stick (0.7 cm diameter) preheated at 100 °C for 10 seconds. Four (4) groups of 8 mice were formed, Control Group (I); Group II, III and IV treated respectively with C. planchonii gel 2.5%, 5%, and Brulex® (Zinc oxide 15% cream). The surfaces of the wounds were evaluated daily by the ImageJ software. Biopsies were performed on day six and twelve for hydroxyproline assays and histological examinations. On the 12th day, wound contraction rates were 44.12%, 67.82%, 66.68% and 69.32% respectively for Group I, Group II, Group III and Group IV. Hydroxyproline levels on day 12 were significantly higher in samples of animals treated with C. planchonii 2.5% and 5% (+114,93 ± 1,61 and +120,54 ± 7,22 of augmentation respectively, P<0.01 when compared to the control group). Histological examination confirmed these observations. C. planchonii root hydroethanolic extract could be a potentially natural remedy for burn wound.

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Pengaruh Papain Getah Pepaya Terhadap Pembentukan Jaringan Granulasi pada Penyembuhan Luka Bakar Tikus Percobaan

Jurnal Kesehatan Andalas ◽

10.25077/jka.v3i1.30 ◽

2014 ◽

Vol 3 (1) ◽

Author(s):

Meishinta Fitria ◽

Deddy Saputra ◽

Gusti Revilla

Keyword(s):

Wound Healing ◽

Granulation Tissue ◽

Control Group ◽

Tissue Formation ◽

Burn Wound ◽

Control Group Design ◽

Granulation Tissue Formation ◽

Burn Wounds ◽

Group Design ◽

Burn Wound Healing

AbstrakLuka bakar merupakan masalah yang serius dalam kesehatan dunia, khususnya di negara berkembang. Di Indonesia belum ada laporan tertulis mengenai jumlah penderita luka bakar dan jumlah angka kematian yang diakibatkannya. Di RSUP Dr. M. Djamil Padang pada tahun 2010 ditemukan 84 kasus luka bakar dengan penyebab sengatan listrik, siraman air panas, kompor, dan minyak panas. Sejumlah studi menunjukkan bahwa tanaman tradisional potensial sebagai agen penyembuhan luka, salah satunya papain getah pepaya (Carica papaya). Penelitian ini bertujuan untuk mengetahui pembentukan jaringan granulasi pada penyembuhan luka bakar tikus percobaan. Jenis penelitian ini adalah penelitian eksperimental dengan rancangan posttest only control group design. Subjek penelitian adalah 10 ekor tikus Wistar jantan yang dibagi menjadi dua kelompok, yaitu kelompok kontrol (K) dan kelompok perlakuan (P). Masing-masing kelompok terdiri dari lima (5) ekor tikus. Plat logam (1,5 cm x 1,5 cm) yang dipanaskan digunakan untuk menghasilkan luka bakar full thickness pada bagian dorsal tikus. Papain getah pepaya diberikan pada kelompok P selama 7 hari. Hasil penelitian ini menunjukkan bahwa pemberian papain getah pepaya tidak memberikan pengaruh yang signifikan (p > 0,05) terhadap pembentukan jaringan granulasi pada penyembuhan luka bakar tikus percobaan. Pada kelompok P didapatkan hasil pembuluh darah 29,26 ± 12,34, fibroblas 26,40 ± 21,94, neutrofil 1,4 ± 0,44, limfosit 1,06 ± 0,13, dan makrofag 1,00 ± 0,00. Kesimpulan penelitian ini adalah papain getah pepaya tidak memberikan pengaruh yang signifikan terhadap pembentukan jaringan granulasi pada penyembuhan luka bakar tikus percobaanKata kunci: papain, jaringan granulasi, luka bakar, penyembuhan lukaAbstractBurn wounds is the serious problem in world health specifically for developing contries. In Indonesia, there is no written report about burn wounds patient and mortality account yet. In 2010, M. Djamil Padang Government Public Hospital found 84 cases of burn wounds with some causes as burn effect from sting of electric, hot water, stove flame and hot oil. Some researches indicate that traditional plant able to be wound healing agent as papaya sap. The purposed of this studi was to find out the effect of papain from papaya sap to granulation tissue formation on burn wounds healing in rat models. This was experimental research with posttest only control group design. The subjects were ten male Wistar rats divided in to two group (control group K and experimental group P). Every group consist of five rats. Heated metal plat (1,5 cm x 1,5 cm) used to get full thickness burn wound on dorsal rat part. Then, papain of papaya sap was given to group P for seven days. The results showed that papain of papaya sap didn’t have significant effect (p > 0,05) to granulation tissue formation in burn wound healing of rat models. In group P, the research found the vascular 29,26 ± 12,24, fibroblast 26,40 ± 21,94, neutrophil 1,4 ± 0,44, lymphocyte 1,06 ± 0,13, and macrophag 1,00 ± 0,00. The conclusion of this research is that papain of papaya sap didn’t have the significant effect to granulation tissue formation in burn wound healing of rat models.Keywords:papain, granulation tissue, burn, wound healing

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Ankaferd blood stopper accelerates deep second degree burn wound healing in rats

Acta Veterinaria Brno ◽

10.2754/avb201887030261 ◽

2018 ◽

Vol 87 (3) ◽

pp. 261-267 ◽

Cited By ~ 1

Author(s):

Ayse Topal ◽

Nihal Y. Gul Satar ◽

I. Taci Cangul ◽

M. Ayberk Oktay ◽

Kivanc Inan ◽

...

Keyword(s):

Wound Healing ◽

Histopathological Examination ◽

Wound Contraction ◽

Control Group ◽

Sprague Dawley ◽

Burn Wound ◽

Burn Wound Healing ◽

Degree Burn ◽

Ankaferd Blood Stopper ◽

The Mean

In this study, the effects of Ankaferd Blood Stopper (ABS) and silver sulphadiazine (SSD) cream on burn wound healing were investigated in rats. A total of 24 outbred, male, Sprague-Dawley rats were randomly allocated to (1) ABS, (2) SSD, and (3) control groups. Bilateral burn wounds were created near the caudal border of the scapula. Wounds in each group were treated daily with sponges soaked in ABS solution, 1% SSD cream, or saline, respectively. On days 0, 7, 14, 21, and 28, unhealed wound area was measured and biopsy samples were taken for histopathological examination (except day 0). At the end of day 28, all rats in the ABS and SSD groups had complete coverage of the wounds with granulation tissue and epithelialization, whereas wounds in the control group were not completely epithelialized. On day 7, the mean unhealed wound areas and the mean percentages of wound contraction were not significantly different among the groups. However, the mean percentage of wound contraction in the ABS and SSD groups was significantly higher than in the control group on days 14, 21, and 28. Histopathologically, wound healing was characterized by a decrease in neutrophil counts and an increase in vessel counts. Our results suggest that ABS can be successfully used for burn wound healing besides SSD.

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