scholarly journals LARVICIDAL ACTIVITY OF ESSENTIAL OIL OF GLIRICIDIA SEPIUM LEAF

Author(s):  
UMADEVI A. ◽  
ABDUL JALEEL

Objective: This study focus at the larvicidal activity of essential oil of Gliricidia sepium leaf Methods: Pharmacological evaluation of leaf was carried out for larvicidal activity. Household mosquitoes of fourth instar larvae were used for the study. The study was carried out for 5 different concentrations for aqueous extract. Results: The aqueous extract shown dose-dependent activity with the maximum larvicidal potential was seen with leaf of essential oil (250 mg/ml). Conclusion: The study is designed to evaluate the larvicidal activity of essential oil of leaf of G. sepium. The relationship between the chemical composition and biological activity of essential oil of G. sepium is confirmed by lethal effect.

2015 ◽  
Vol 70 (1-2) ◽  
pp. 1-6 ◽  
Author(s):  
Xin Chao Liu ◽  
Qiyong Liu ◽  
Xu Bo Chen ◽  
Qi Zhi Liu ◽  
Zhi Long Liu

Abstract The aim of this study was to evaluate the larvicidal activity of the essential oil of Youngia japonica aerial parts against the larvae of Aedes albopictus and to isolate any active compounds from the oil. Gas chromatography-mass spectrometry (GC-MS) analyses revealed the presence of 31 compounds, with menthol (23.53%), α-asarone (21.54%), 1,8-cineole (5.36%), and caryophyllene (4.45%) as the major constituents. Bioactivity-directed chromatographic separation of the oil led to the isolation of menthol and α-asarone as active compounds. The essential oil of Y. japonica exhibited larvicidal activity against the fourth instar larvae of A. albopictus with an LC50 value of 32.45 μg/mL. α-Asarone and menthol possessed larvicidal activity against the fourth instar larvae of A. albopictus with LC50 values of 24.56 μg/mL and 77.97 μg/mL, respectively. The results indicate that the essential oil of Y. japonica aerial parts and the two constituents can be potential sources of natural larvicides.


2021 ◽  
Vol 10 (1) ◽  
pp. e35710111166
Author(s):  
Thércia Gabrielle Teixeira Martins ◽  
Paulo Victor Serra Rosa ◽  
Mariana Oliveira Arruda ◽  
Andressa Almeida Santana Dias ◽  
Ari Pereira de Araújo Neto ◽  
...  

This study aimed to evaluate the larvicidal activity of essential oil microparticles (EO) of Melissa officinalis L. against Aedes aegypti. The leaves of M. officinalis were collected in the municipality of São José de Ribamar, Maranhão, Brazil, later dried, crushed and ground. 90g of the dried leaves were used to obtain the EO by the hydrodistillation method. For the synthesis of microencapsulated EO, 60g of sodium alginate (2.5% m/v) was added to the mixture of 15g of Tween 20 with 6g of EO. The mixture was homogenized and drips over CaCl2 5% m/v solution for the hardening of particles via crosslinking. The microparticles were washed with distilled water in filter and dried at 35ºC/24h and 15 days at tamb (30ºC). The eggs of Aedes aegypti were collected at the Federal University of Maranhão by the ovitrampas method. The larvae that hatched were fed until they reached the fourth instar. Groups of larvae (n=20) were submitted to solutions of EO and microparticles of 10-90 mg/L . After 24 h, live and dead larvae were counted and LC50 was calculated by the Reed&Muench method, using Cheng's criterion for classification of active potential. All larvae presented mortality in all concentrations tested. The LC50 obtained for the EO was 40.60 mg/L and for the microparticles 22.10 mg/L, both classified as active according to the adopted criterion, but it is observed that the microparticles increased the larvicidal potential of the EO. Through the results obtained, it is concluded that the microparticles formulated with the EO proved to be efficient in the face of the larvae of Aedes aegypti, being interesting and important in controlling and combating the mosquito that transmits dengue.


2010 ◽  
Vol 13 (3) ◽  
pp. 29-38
Author(s):  
Ha Bich Phan ◽  
Thach Ngoc Le

Coriandrum sativum L. belongs to the Apiaceae family, which is cultivated in Dong Nai province, in this paper its seed oil of was studied. The essential oil was extracted by hydrodistillation. We used two methods for activating of hydrodistillation: conventional heating and microwave irradiating. Its physical and chemical indexes were measured. The chemical composition of this oil was identified by GC/MS and quantified by GC/FID. Linalool (75.51-77.21 %), and geranyl acetate (15.64-12.79 %) were the main constituents of the oil which were obtained in 0,32-0.39 % yield. The biological activity of this oil was reported.


Author(s):  
Noorzaid Muhamad ◽  
Syahirah Sazeli ◽  
Resni Mona ◽  
Jannathul Firdous

The anthelmintic resistance has limited the control of gastrointestinal nematodes of small ruminants and thus has awakened interest in the study of plants extract as a source of anthelmintics. These experiments were carried out to evaluate the in vitro efficacy of Jatrophacurcas latex extract against Haemonchuscontortus larval motility. To evaluate the larvicidal activity, H.contortus L3 were incubated with the extracts with varying concentration of 5 mg/mL, 10 mg/mL, 15 mg/mL and 20 mg/mL at 27°C for 48, 72 and 96 hrs. The results were subjected to the Kruskal-Wallis test (P less than 0.05). The extracts showed dose-dependent larvicidal effects. These results suggest that J.curcas can be used to control gastrointestinal nematodes of small ruminants.


2017 ◽  
Vol 04 (1) ◽  
pp. 43-49
Author(s):  
Amir Reza Jassbi ◽  
Mehdi Zare ◽  
Fatemeh Heidary Jamebozorgi

Author(s):  
Retno Widyowati ◽  
Suciati Suciati ◽  
Dewi Melani Haryadi ◽  
Hsin-I Chang ◽  
IPG Ngurah Suryawan ◽  
...  

Abstract Objectives Glucocorticoid-induced osteoporosis (dexamethasone) is a primary cause of secondary osteoporosis by the decreasing formation and increasing resorption activities. Previously, the in vitro study showed that 70% ethanol and aqueous extract of deer antler have increased alkaline phosphatase in osteoblast cell that known as marker of bone formation. The mind of this study is to analyze the effect of deer antlers in increasing the bone trabecular density of osteoporosis-induced male mice. Methods This study used a post-test control group design. A total of 54 healthy male mice were randomly divided to nine groups, i.e., healthy control, osteoporotic, positive control, 70% ethanol (4, 8, and 12 mg/kg BW), and aqueous extracts (4, 8, and 12 mg/kg BW) of deer antler groups. All of the interventions were given 1 mL of test sample for 4 weeks orally. The bone densities were determined using histomorphometry by Image J and Adobe Photoshop. The statistical data were performed using SPSS 23 and statistical significance was set at p<0.05. Results The results showed that alendronate group, 70% ethanol, and aqueous extract groups increased bone density and calcium levels in serum (p<0.05) compared to osteoporotic group in dose dependent manner. It indicated that 70% ethanol and aqueous extract of deer antler stimulating bone turnover and aqueous extract showed the highest. Conclusions Dexamethasone induction for 4 weeks caused osteoporotic mice and the administration of 70% ethanol and aqueous extracts of deer antler from East Kalimantan increased trabecular bone density and calcium levels in dose dependent manner.


Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1303 ◽  
Author(s):  
Do N. Dai ◽  
Nguyen T. Chung ◽  
Le T. Huong ◽  
Nguyen H. Hung ◽  
Dao T.M. Chau ◽  
...  

Members of the genus Cinnamomum (Lauraceae) have aromatic volatiles in their leaves and bark and some species are commercially important herbs and spices. In this work, the essential oils from five species of Cinnamomum (C. damhaensis, C. longipetiolatum, C. ovatum, C. polyadelphum and C. tonkinense) growing wild in north central Vietnam were obtained by hydrodistillation, analyzed by gas chromatography and screened for antimicrobial and mosquito larvicidal activity. The leaf essential oil of C. tonkinense, rich in β-phellandrene (23.1%) and linalool (32.2%), showed excellent antimicrobial activity (MIC of 32 μg/mL against Enterococcus faecalis and Candida albicans) and larvicidal activity (24 h LC50 of 17.4 μg/mL on Aedes aegypti and 14.1 μg/mL against Culex quinquefasciatus). Cinnamomum polyadelphum leaf essential oil also showed notable antimicrobial activity against Gram-positive bacteria and mosquito larvicidal activity, attributable to relatively high concentrations of neral (11.7%) and geranial (16.6%). Thus, members of the genus Cinnamomum from Vietnam have shown promise as antimicrobial agents and as potential vector control agents for mosquitoes.


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