Antibacterial and Antibiofilm Activity of Aqueous Extract and Essential Oil of Origanummajorana and their Activity on Some Physiological Parameters of Blood in the Male of White Rats

2020 ◽  
Vol 11 (2) ◽  
pp. 2098
Author(s):  
Nibras Y. Abdulla ◽  
Baydaa A. Hassan
Keyword(s):  
Essential Oil ◽  
Aqueous Extract ◽  
Physiological Parameters ◽  
Antibiofilm Activity ◽  
White Rats

Antibacterial and antibiofilm activity of essential oil of clove against Listeria monocytogenes and Salmonella Enteritidis

2021 ◽  
pp. 108201322110132
Author(s):  
Mariem Somrani ◽  
Hajer Debbabi ◽  
Alfredo Palop
Keyword(s):  
Cell Adhesion ◽  
Listeria Monocytogenes ◽  
Essential Oil ◽  
Salmonella Enteritidis ◽  
Bioactive Compound ◽  
Gas Chromatography Mass Spectrometry ◽  
Antibiofilm Activity ◽  
Disk Diffusion Assay ◽  
Clove Essential Oil ◽  
Potential Strategy

The antibacterial and antibiofilm activity of essential oil of clove against Listeria monocytogenes and Salmonella Enteritidis were investigated. The chemical composition of the oil was characterized by gas chromatography–mass spectrometry. Stock solution of the essential oil of clove was prepared in 95% (v/v) ethanol (EOC). The antibacterial assays were performed by disk diffusion assay and minimal inhibitory concentration (MIC). The biomass of adhered cells and preformed biofilms after incubation with different concentrations of EOC was assessed by crystal violet. Eugenol was the major bioactive compound of clove essential oil, accounting for 78.85% of the total composition. The MIC values for L. monocytogenes and S. Enteritidis were 0.05 mg/ml and 0.1 mg/ml, respectively. The initial cell adhesion at MIC was inhibited by 61.8% for L. monocytogenes and 49.8% for S. Enteritidis. However, the effect of EOC was less marked on biofilm eradication than on cell adhesion. At MIC and within 1 hour of incubation with the EOC, the preformed biofilms were reduced by 30.2% and 20.3% for L. monocytogenes and S. Enteritidis, respectively. These results suggest that sanitizers based on clove essential oil could be a potential strategy to control biofilms in food-related environments.

scholarly journals Studies on the toxicity of an aqueous extract of the leaves of Abrus precatorius in rats

2007 ◽  
Vol 74 (1) ◽  
Author(s):  
A.A. Adedapo ◽  
O.A. Omoloye ◽  
O.G. Ohore
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Aqueous Extract ◽  
Haemoglobin Concentration ◽  
Total Serum Protein ◽  
Total Serum ◽  
Blood Cell Count ◽  
Abrus Precatorius ◽  
White Rats ◽  
Histopathological Studies

The toxic effects of an aqueous extract of Abrus precatorius were studied in 20 male white rats over a period of 18 days. The rats were divided into four groups of five rats per group. Those in Group A served as controls while the rats in Groups B, C and D were dosed per os with 400 mg/kg, 800 mg/kg and 1 600 mg/kg of the extract, respectively. Blood samples were collected for haematological and biochemical analysis and specimens of the liver, kidney and testes were taken for histopathological studies. The study showed that the extract of A. precatorius caused decreased levels of packed cell volume, haemoglobin concentration, red blood cell count, white blood cell count, mean corpuscular volume and mean corpuscular haemoglobin. The extract also resulted in increased levels of total serum protein, albumin, alanine amino transaminase, aspartate amino transferase, alkaline phosphatase and total bilirubin. Histologically, testicular degeneration characterized by decreased numbers of lining cells of the epithelium as well as reduction in sperm cells with presence of scattered Sertoli cells were noted. The study thus showed that aqueous extract of Abrus precatorius is toxic and caution should be exercised in its use for medicinal purpose.

scholarly journals Aromatherapy with ylang ylang for anxiety and self-esteem: a pilot study

2014 ◽  
Vol 48 (3) ◽  
pp. 492-499 ◽  
Author(s):  
Juliana Rizzo Gnatta ◽  
Patricia Petrone Piason ◽  
Cristiane de Lion Botero Couto Lopes ◽  
Noemi Marisa Brunet Rogenski ◽  
Maria Júlia Paes da Silva
Keyword(s):  
Pilot Study ◽  
Essential Oil ◽  
Self Esteem ◽  
Physiological Parameters ◽  
Post Intervention ◽  
Temperature Method ◽  
Significant Difference ◽  
Perception Of Self ◽  
Cutaneous Application

Objective: To verify if the use of ylang ylang essential oil by cutaneous application or inhalation alters the anxiety and self-esteem perception and physiological parameters as blood pressure and temperature. Method : A pilot study with 34 professionals from a nursing group randomized in three groups: one received the ylang ylang essential oil by cutaneous application, the second received through inhalation and the third (placebo) received the ylang ylang essence through cutaneous application. The assessment was done by an Anxiety Inventory (IDATE) and the Dela Coleta self-esteem scale, applied on baseline, after 30, 60 and 90 days and after 15 days post-intervention (follow up). Results : In the pre and post-intervention intergroup analysis, there was a significant difference in self-esteem for the three groups (p values: G1=0.014; G2=0.016; G3=0.038). There were no differences in the analysis between groups for anxiety or for physiological parameters. Conclusion : It was found significant alterations only to the intergroup perception of self-esteem for the three groups.


scholarly journals Kaempferia galanga L. Rhizome As a Potential Dental Plaque Preventive Agent

2010 ◽  
Vol 1 (1) ◽  
pp. 19
Author(s):  
Triana Hertiani ◽  
Sylvia Utami Tunjung Pratiwi ◽  
Iramie Duma Kencana Irianto ◽  
Aini Febriana
Keyword(s):  
Essential Oil ◽  
Inhibitory Activity ◽  
Dental Plaque ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antibiofilm Activity ◽  
Kaempferia Galanga ◽  
Degradation Activity ◽  
Biofilm Inhibitor

Dental plaque prevention can be achieved by inhibition of mouth cavity microbes to built biofilm. Kaempferia galanga rhizome has been known as a potential antibacterial agent. This research aimed to reveal the potency of Kaempferia galanga extract and essential oil as anti plaque active agents, based on their in vitro inhibitory activity against the planktonic growth and biofilm of Streptococcus mutans ATCC 21752. Kaempferia galanga extract was obtained by defatting dried-pulverized samples in petroleum ether prior to immersion in 70% ethanol. The fresh rhizome was steam-hydro distilled for 6 h to yield the essential oil. Antibacterial and anti biofilm assays were measured by micro dilution technique on polystyrene 96-wells micro titer plates at 37°C. The percentage of inhibition was calculated by comparing the absorbance of samples to the vehicle (control) measured by micro plate reader at 595 nm. Biofilms formed were first stained by 1% crystal violet. The above assays were performed in triplicates. This study revealed that both K. galanga rhizome essential oil and ethanolic extract showed antibacterial and antibiofilm activity towards S. mutans. The ethanol extract showed MIC90 value at 0.091% w/v and MBC at 2.724% w/v for antibacterial activity; IC50 at 0.048 % w/v for anti biofilm formation activity; and EC50 at 0.052%w/v for biofilm degradation activity. Until the highest concentration tested (0.6%w/v), the MIC90 and MBC values of the essential oil were not revealed, but higher biofilm inhibitory activity i.e. IC50 at 0.025 % w/v; and EC50 at 0.034 %w/v were observed. Key words: biofilm inhibitor, antibacterial, Kaempferia galanga

Phytotoxic effects of extract and essential oil of Eucalyptus saligna (Myrtaceae) leaf litter on grassland species

2017 ◽  
Vol 65 (2) ◽  
pp. 172 ◽  
Author(s):  
Eliane R. Silva ◽  
Diana C. Lazarotto ◽  
Joséli Schwambach ◽  
Gerhard E. Overbeck ◽  
Geraldo L. G. Soares
Keyword(s):  
Essential Oil ◽  
Leaf Litter ◽  
Aqueous Extract ◽  
Seedling Growth ◽  
Electrolyte Leakage ◽  
Membrane Damage ◽  
Initial Growth ◽  
Eucalyptus Saligna ◽  
Allelopathic Potential ◽  
Grassland Species

Under Eucalyptus plantations in the South Brazilian grassland region, few plants establish, and allelopathy may be involved in shaping this pattern. We aimed to assess the phytotoxicity of essential oil and aqueous extract of Eucalyptus saligna Sm. leaf litter on grassland species. We tested the effects of E. saligna pure oil and extract on germination, seedling growth, H2O2 levels and electrolyte leakage of seedling membranes of Paspalum notatum Flüggé, Eragrostis plana Ness (Poaceae), Trifolium repens L. and Lotus corniculatus L. (Fabaceae). Essential oil and aqueous extract of E. saligna affected all recipient species, even at the lowest amounts/concentrations, inhibiting germination and initial growth, and also increasing H2O2 levels and electrolyte leakage of seedling membranes. Essential oil consisted mainly of monoterpenes and presented α-pinene and 1,8-cineole as the major compounds. The extract contained phenolics, and lower levels of these compounds in the extract were associated with decreased phytotoxicity. We conclude that E. saligna contains phytotoxic compounds in leaf litter that generate oxidative stress and lead to membrane damage, affecting seeds and seedling growth. In addition, we relate E. saligna phytotoxicity to monoterpenes that may be released from leaf litter by volatilisation, and phenolics that may be leached by rainfall. Our study indicates that E. saligna has allelopathic potential on the tested grassland species.

scholarly journals Effect of peppermint and thyme essential oil mist on performance and physiological parameters in broiler chickens

2019 ◽  
Vol 49 (1) ◽  
pp. 29 ◽  
Author(s):  
D Witkowska ◽  
J Sowińska ◽  
D Murawska ◽  
P Matusevičius ◽  
A Kwiatkowska-Stenzel ◽  
...  
Keyword(s):  
Essential Oil ◽  
Broiler Chickens ◽  
Physiological Parameters ◽  
Thyme Essential Oil ◽  
Oil Mist

scholarly journals Biological Activity and Antibiofilm Molecular Profile of Citrus aurantium Essential Oil and Its Application in a Food Model

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3956 ◽  
Author(s):  
Miroslava Kačániová ◽  
Margarita Terentjeva ◽  
Lucia Galovičová ◽  
Eva Ivanišová ◽  
Jana Štefániková ◽  
...  
Keyword(s):  
Essential Oil ◽  
Vapor Phase ◽  
Molecular Profile ◽  
Quantitative Composition ◽  
Antibiofilm Activity ◽  
Citrus Aurantium ◽  
Microdilution Method ◽  
Dpph Method ◽  
The Media ◽  
Glass Surfaces

The main aim of the study was to investigate the chemical composition, antioxidant, antimicrobial, and antibiofilm activity of Citrus aurantium essential oil (CAEO). The biofilm profile of Stenotrophonomonas maltophilia and Bacillus subtilis were assessed using the mass spectrometry MALDI-TOF MS Biotyper and the antibiofilm activity of Citrus aurantium (CAEO) was studied on wood and glass surfaces. A semi-quantitative composition using a modified version was applied for the CAEO characterization. The antioxidant activity of CAEO was determined using the DPPH method. The antimicrobial activity was analyzed by disc diffusion for two biofilm producing bacteria, while the vapor phase was used for three penicillia. The antibiofilm activity was observed with the agar microdilution method. The molecular differences of biofilm formation on different days were analyzed, and the genetic similarity was studied with dendrograms constructed from MSP spectra to illustrate the grouping profiles of S. maltophilia and B. subtilis. A differentiated branch was obtained for early growth variants of S. maltophilia for planktonic cells and all experimental groups. The time span can be reported for the grouping pattern of B. subtilis preferentially when comparing to the media matrix, but without clear differences among variants. Furthermore, the minimum inhibitory doses of the CAEO were investigated against microscopic fungi. The results showed that CAEO was most active against Penicillium crustosum, in the vapor phase, on bread and carrot in situ.

scholarly journals Pullulan Films Containing Rockrose Essential Oil for Potential Food Packaging Applications

Antibiotics ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 681
Author(s):  
Ângelo Luís ◽  
Ana Ramos ◽  
Fernanda Domingues
Keyword(s):  
Essential Oil ◽  
Shelf Life ◽  
Bacterial Adhesion ◽  
Food Packaging ◽  
Bornyl Acetate ◽  
Antibiofilm Activity ◽  
Gram Negative Bacteria ◽  
Violacein Production ◽  
Potential Food ◽  
Microscopy Images

Active packaging is designed to control the development of decay- and disease-causing microorganisms and is emerging as a promising technology for extending shelf-life, maintaining food safety, reducing waste, and minimizing the risks for foodborne diseases. The goal of this work was to develop and characterize bioactive pullulan-based films, containing rockrose (Cistus ladanifer) essential oil. Among other abundant compounds (camphene, bornyl acetate and trans-pinocarveol), α-pinene was identified as the major compound of rockrose essential oil (39.25%). The essential oil presented stronger antibacterial activity against Gram-positive than against Gram-negative bacteria. The antioxidant results indicate the potential of the developed films to be used to package foods susceptible to oxidation and rancification, thus improving their shelf-life. Also, this study reflects the potential of rockrose essential oil, free or incorporated in pullulan, as a promising quorum sensing inhibitor, since it was able to interrupt intercellular communication, inhibiting violacein production. Electronic microscopy images showed the antibiofilm activity of the films with rockrose essential oil that were able to influence bacterial adhesion, which may be explained by the differences in the surface free energy of the films, as also determined.

scholarly journals Antioxidant, Antimicrobial and Antibiofilm Activity of Coriander (Coriandrum sativum L.) Essential Oil for Its Application in Foods

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 282 ◽  
Author(s):  
Miroslava Kačániová ◽  
Lucia Galovičová ◽  
Eva Ivanišová ◽  
Nenad L. Vukovic ◽  
Jana Štefániková ◽  
...  
Keyword(s):  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Early Growth ◽  
Coriandrum Sativum ◽  
Penicillium Expansum ◽  
Antibiofilm Activity ◽  
Planktonic Cells ◽  
Grouping Pattern ◽  
The Media

The aim of this study was to assess the chemical composition, antioxidant, antimicrobial and antibiofilm activity of the Coriandrum sativum essential oil. Changes in the biofilm profile of Stenotropomonas maltophilia and Bacillus subtilis were studied using MALDI-TOF MS Biotyper on glass and wooden surfaces. The molecular differences of biofilms in different days were observed as well. The major volatile compounds of the coriander essential oil in the present study were β-linalool 66.07%. Coriander essential oil radical scavenging activity was 51.05% of inhibition. Coriander essential oil expressed the strongest antibacterial activity against B. subtilis followed by S. maltophilia and Penicillium expansum. The strongest antibiofilm activity of the coriander essential oil was found against S. maltophilia. A clearly differentiated branch was obtained for early growth variants of S. maltophilia in case of planktonic cells and all experimental groups and time span can be reported for the grouping pattern of B. subtilis preferentially when comparing to the media matrix, but without clear differences among variants. The results indicate that coriander was effective against the tested Penicillium expansum in the vapor phase after 14 days with MID50 367.19 and MID90 445.92 µL/L of air.

Sign in / Sign up

Export Citation Format

Share Document