scholarly journals INVESTIGATION OF ANTHELMINTIC EFFECT OF NEOLAMARCKIA CADAMBA FRUIT EXTRACTS IN ASCARIASIS

2021 ◽  
pp. 70-75
Author(s):  
KULDEEP KAUR ◽  
AJEET PAL SINGH ◽  
AMAR PAL SINGH
Keyword(s):  
Conventional Medicine ◽  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Phenyl Ether ◽  
Time To Death ◽  
Fruit Extracts ◽  
Dose Levels ◽  
Objective Investigation ◽  
Anthelmintic Effect

Objective: Investigation of Anthelmintic effect of Neolamarckia Cadamba fruit extracts (Ethanol, Aquaous, Phenyl ether, Chloroform) in Ascariasis. Methods: The experiment was conducted on an adult Indian earthworm, Eiseniafoetida, owing of its morphological and physiological similarities to the human intestine roundworm parasite. Six sets of six earthworms were discharged sequentially into different extracts of N. cadamba fruits at dose levels of 5,10,15,20, and 25 mg/ml, respectively, and 25 mg/ml of albendazole solutions. Albendazole was employed as the standard reference medication. Results: The results showed that Ethanolic extract has the greatest degree of activity. When compared to any other extract or conventional medicine, it produces a paralytic effect sooner and has a shorter time to death (Albendazole). An ethanol extract included alkaloid, saponin, tannins, flavonoids, proteins, and amino acids. As a result, it is possible to infer that the ethenolic fruit extract of Neolomarckia Cadamba showed much more anthelmintic activity against Indian earthworms than the usual treatment (Albendazole). Conclusion: The solvents Ethanol, Chloroform, Phenyl Ether, and Aquaous were used to extract the Neolamarckia Cadamba. The results showed that Ethanolic extract has the greatest degree of activity. When compared to any other extract or conventional medicine, it produces a paralytic effect sooner and has a shorter time to death (Albendazole).

scholarly journals IN VITRO ANTHELMINTIC AND ANTI-AMYLASE PROPERTIES OF GARCINIA PEDUNCULATA ROXB. ETHANOLIC EXTRACT

2016 ◽  
Vol 9 (1) ◽  
pp. 189
Author(s):  
Pinky Sarmah ◽  
Nako Kobing ◽  
Jyotchna Gogoi ◽  
Ananta Madhab Dutta
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Biological Properties ◽  
Death Time ◽  
Control Sets ◽  
Point Analysis ◽  
Soxhlet Apparatus ◽  
And Control

<p><strong>Objective</strong>:<strong> </strong>The present study aims to investigate <em>in vitro</em> anthelmintic and anti-amylase properties of the ethanolic fruit extract of <em>Garcinia pedunculata</em>.</p><p><strong>Methods</strong>:<strong> </strong>For the study, mature fruits of <em>G. pedunculata</em> were collected from local markets during April-May. Fleshy pericarps of fruits were chopped into small pieces, dried and extracted by using a Soxhlet apparatus. Ethanol extract of <em>G. pedunculata</em> was used for evaluation of <em>in vitro</em> anthelmintic and anti-amylase activities. <em>In vitro</em> anthelmintic activity was evaluated in animal models, <em>Pheretima posthuma</em>, an earthworm species. <em>In vitro</em> anti-amylase activity was evaluated by using zymographic, achromic point analysis (Starch-Iodine method) and spectrophotometric method [Di Nitro Salicylic acid (DNS)–Maltose method].</p><p><strong>Results</strong>:<strong> </strong>Ethanolic extract of <em>G. pedunculata</em> showed anthelmintic activity at a concentration of 75 mg/ml, paralysis and death timing was reported at 0.62±0.26 min and 1.42±0.07 min, respectively. The reference standard (Albendazole) showed paralysis time: 2.13±0.28 min and death time: 5.12±0.29 min. In the anti-amylase study, a zymographic density analysis of <em>G. pedunculata</em> showed significant variation in band intensity as compared to Starch–Iodine achromic point analysis and DNS–Maltose method. A concentration of 1.5 mg/ml of extract showed inhibition of amylase: 67.65±1.53 % as compared to other concentrations and control sets.</p><strong>Conclusion</strong>:<strong> </strong>It could be concluded that ethanolic extract of <em>G. pedunculata</em>has biological properties which could be utilised in medicine by characterising its bioactive components.<p> </p>

scholarly journals The Anthelmintic Activity Of Etanol Extract of Porang Tubers Against Fasciola gigantica In Vitro

2021 ◽  
Vol 10 (2) ◽  
pp. 66
Author(s):  
Firanda Agustina ◽  
Rochmah Kurnijasanti ◽  
Lucia Tri Suwanti ◽  
Nunuk Dyah Retno Lastuti ◽  
Kadek Rachmawati ◽  
...  
Keyword(s):  
Microscopic Examination ◽  
Immersion Time ◽  
Morphological Changes ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Fasciola Gigantica ◽  
Histopathological Changes ◽  
Extract Concentration ◽  
Anthelmintic Effect

This study aims to determine the anthelmintic activity ethanol extract of porang tubers (A. oncophillus) against F. gigantica worms in vitro. There were five treatments and each treatment was done in five replications and used 10 F. gigantica. The treatments of this research were K- with CMC Na 1%, K+ with Albendazole 2.4 mg/ml, P1 with extract concentration 5%, P2 with extract concentration 10%, P3 with extract concentration 20%. The results showed that the extract of Porang tuber (A. oncophillus) had an anthelmintic effect against F. gigantica worms in vitro. In the extract with a concentration of 20%, there were anthelmintic properties that almost the same as Albendazole. The higher the extract concentration, the higher the anthelmintic properties. The longer the immersion time, the higher the number of dead worms. The morphological changes was evaluated by light microscopic examination and the results showed many histopathological changes on the morphology of F. gigantica. The results indicate that the possible use of the tubers as a potential anthelmintic against F. gigantica.

scholarly journals Evaluation of Anthelmintic Activity of Calendula officinalis Flowers Extract

2021 ◽  
Vol 11 (4) ◽  
pp. 48-50
Author(s):  
Alok Bhardwaj ◽  
Khursheed Alam ◽  
Devender Pathak ◽  
Shahid Ansari ◽  
Vandana Arora Sethi
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Chemical Investigation ◽  
Calendula Officinalis ◽  
Pharmacological Activities ◽  
Ethanolic Extracts ◽  
Level Of Significance ◽  
Dose Dependent ◽  
One Way Anova

Background: The present study is an attempt to explore the anthelmintic activity of ethanolic extract of flower of Calendula officinalis. Various doses of ethanolic extracts were evaluated for their anthelmintic activity on adult Indian earthworms, Pheretima posthuma. Calendula officinalis Linn has been widely used in homeopathic medicine for the treatment of many diseases. It has been reported to possess many pharmacological activities, which include antibacterial, antifungal, antiviral,antioxidant and anti-inflammatory. Methods: The ethanol extract of air dried flowers powder (500g) was prepared by using soxlet apparatus, concentrated and vacuum dried which gave an orange yellowish mass (60.46 g). Extract was subjected to qualitative chemical investigation of phytoconstituents such as glycosides, alkaloids, flavonoids, carbohydrates, tannins, proteins, vitamins, coumarins etc. Results: All extracts were able to show anthelmintic activity at 10mg/mL concentration. The activities are comparable with the standard drugs, piperazine citrate and albendazole. The data were found statistically significantly by using one way Anova at 5% level of significance (p< 0.05). Conclusion: All the doses of ethanolic extract of calendula officinalis showed dose dependent anthelmintic activity in comparison to standard drugs. Keywords: Calendula officinalis, Asteraceae, Anthelmintic, Piperazine citrate, Albendazole

scholarly journals Assessment of Anthelmintic Property and Insilico study of phytocompounds in roots of Dechaschistia crotonifolia Wight & Arn.

2022 ◽  
Author(s):  
Raveesha Peeriga ◽  
Keerthi Priyanka Adarapu ◽  
Kavya Sri Sanivar ◽  
Jyothsna Kanumuri ◽  
Rikith Swamy Akunuri ◽  
...  
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Tween 80 ◽  
Docking Studies ◽  
Vital Role ◽  
Standard Drug ◽  
Death Time ◽  
The Family ◽  
And Control

Abstract Background:Worm infections in developing countries were reported high. Phytoconstituents have been a vital role for the treatment of many ailments. The current study was aimed assess for anthelmintic activity of different root extracts of Dechaschistia crotonifolia Wight & Arn. belongs to the family Ebanaceae against Pheretima posthuma. Further Insilico study was carried out for phytocompounds present in Dechaschistia. Results: The chloroform, ethylacetate and ethanol extract of Dechaschistia crotonifolia Wight & Arn. were considered for the study of anthelmintic property on earthworms at concentrations 20 mg/ml, 40 mg/ml and 60 mg/ml. During this study, the parameters paralysis time (Pt) and Death Time (Dt) of adult Indian earthworms was observed. As a standard and control Albendazole 10 mg/ml and 2% Tween 80 in distilled water were taken respectively. The study resulted that ethanolic extract was significant when compared with the Albendazole 10 mg/ml. Docking studies revealed the all phytocompounds in Dechaschistia shown binding affinity, however comparatively scopoletin and stigmasterol had shown a good binding affinitiy about -7.7 Kcal/mol and -7.6 Kcal/mol compared to standard drug Albendazole which was shown about -8.7 Kcal/mol. Conclusion: The study revealed that the ethanol extract of Dechaschistia crotonifolia Wight & Arn. at a concentration of 60mg/ml exhibited a stronger anthelmintic property compared to Albendazole 10mg/ml. A dose dependent anthelmintic activity is exerted by all the extracts in an ascending manner Chloroform<Ethyl acetate<Ethanol. These observations were made evidenced by docking studies of phytocompounds in Dechaschistia as the phytocompounds were shown excellent docking score when compared with standard Albendazole.

IN VITRO ANTHELMINTIC ACTIVITY OF ARTEMISIA MARITIMA LINN.

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (08) ◽  
pp. 39-41
Author(s):  
M. M Hayat ◽  
◽  
S Sharma ◽  
D Vashishth ◽  
R. Kaur
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Aqueous Extracts ◽  
Reference Standard ◽  
Dose Levels ◽  
Artemisia Maritima

Ethanolic and aqueous extracts of the flower heads of Artemisia maritima Linn. were evaluated for anthelmintic activity on Indian earthworms Pheritima posthuma, using albendazole as reference standard. The extracts caused paralysis followed by death of the worms at all tested dose levels. Ethanolic extract was found to be more potent than the reference control albendazole. The result supports the traditional claims of Artimisia maritime as a powerful anthelmintic.

scholarly journals Evaluation of phytochemical, anthelmintic activity and antidiabetic activity-melochia corchorifolia ethanol extract

2021 ◽  
Vol 12 (1) ◽  
pp. 234-239
Author(s):  
Vinoth M ◽  
Natarajan B
Keyword(s):  
Folk Medicine ◽  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Soxhlet Apparatus ◽  
Maximum Inhibition ◽  
Anti Cancer ◽  
Cytotoxicity Effects

Melochia corchorifolia belongs to the Sterculiaceae family and is a common weed distributed throughout tropical and subtropical regions in many countries. Melochia corchorifolia is a medicinal plant which is used as a traditional folk medicine for the treatment of the various diseases. Several studies have reported antibacterial, antifungal, cytotoxicity effects and anti-cancer activity of water and methanol extracts of the leaf. The dried leaves were powdered and extracted with ethanol solvent through the Soxhlet apparatus. The phytochemical analysis was done using standard techniques on the ethanolic extract. The analysis showed the presence of various phytoconstituents such as flavonoids, alkaloids, steroids, terpenoids, glycosides, tannins, phenols, saponins and anthraquinones. Besides, the research was aimed at assessing the Anthelmintic behaviour of ethanol extract of Melochia corchorifolia leaves against Eisenia fetida (Indian earthworms)  the result shown that 300 mg/ml proved to be very active by paralyzing and killing the earthworms in a shorter time and followed by  Antidiabetic activity in  inhibition assay for α-amylase activity was evaluated using Melochia corchorifolia leaves showed maximum inhibition of the enzyme with the highest value of 85.0% seen at 100mg/ml. Our results reveal that ethanol extract of the leaf of Melochia corchorifolia acquires potent bioactive phytocompounds that might be developed into novel Anthelmintic and Antidiabetic activities.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

In Silico Studies on Anti-Stress Compounds of Ethanolic Root Extract of Hemidesmus indicus L.

2020 ◽  
Vol 21 (6) ◽  
pp. 502-515
Author(s):  
Jayasimha R. Daddam ◽  
Basha Sreenivasulu ◽  
Katike Umamahesh ◽  
Kotha Peddanna ◽  
Dowlathabad M. Rao
Keyword(s):  
Conventional Medicine ◽  
Herbal Medicines ◽  
Ethanolic Extract ◽  
Data Bank ◽  
Docking Studies ◽  
Dynamics Simulation ◽  
Alternative Methods ◽  
Root Extract ◽  
Hemidesmus Indicus ◽  
In Silico Studies

Background: Alternative medicine is available for those diseases which cannot be treated by conventional medicine. Ayurveda and herbal medicines are important alternative methods in which the treatment is done with extracts of different medicinal plants. This work is concerned with the evaluation of anti-stress bioactive compounds from the ethanolic root extract of Hemidesmus indicus. Methods: Gas chromatography and Mass Spectrum studies are used to identify the compounds present in the ethanolic extract based on the retention time, area. In order to perform docking studies, Vasopressin model is generated using modeling by Modeller 9v7. Vasopressin structure is developed based on the crystal structure of neurophysin-oxytocin from Bos taurus (PDB ID: 1NPO_A) collected from the PDB data bank. Using molecular dynamics simulation methods, the final predicted structure is obtained and further analyzed by verifying 3D and PROCHECK programs, confirmed that the final model is reliable. The identified compounds are docked to vasopressin for the prediction of anti-stress activity using GOLD 3.0.1 software. Results: The predicted model of Vasopressin structure is stabilized and confirmed that it is a reliable structure for docking studies. The results indicated ARG4, THR7, ASP9, ASP26, ALA32, ALA 80 in Vasopressin are important determinant residues in binding as they have strong hydrogen bonding with phytocompounds. Among the 21 phytocompounds identified and docked, molecule Deoxiinositol, pentakis- O-(trimethylsilyl) showed the best docking results with Vasopressin. Conclusion: The identified compounds were used for anti-stress activity by insilico method with Vasopressin which plays an important role in causing stress and hence selected for inhibitory studies with phytocompounds. The phytocompounds are inhibiting vasopressin through hydrogen bodings and are important in protein-ligand interactions. Docking results showed that out of twenty-one compounds, Deoxiinositol, pentakis-O-(trimethylsilyl) showed best docking energy to the Vasopressin.

In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

2016 ◽  
Vol 5 (04) ◽  
pp. 4512
Author(s):  
Jackie K. Obey ◽  
Anthoney Swamy T* ◽  
Lasiti Timothy ◽  
Makani Rachel
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity ◽  
Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

scholarly journals Ekstrak Etanol Kulit Buah Naga Menurunkan Indikasi Neoplasia Mammae Tikus Putih Berdasarkan Histopatologi dan Inhibitor Siklooksigenase-2

2018 ◽  
Vol 19 (1) ◽  
pp. 23
Author(s):  
Muhammad Thohawi Elziyad Purnama ◽  
Ragil Angga Prastiya ◽  
Faisal Fikri ◽  
Amung Logam Saputro ◽  
Bodhi Agustono
Keyword(s):  
Mammary Gland ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Growth Function ◽  
Histopathological Features ◽  
White Rats ◽  
Fruit Skin ◽  
Dragon Fruit ◽  
Cox 2 ◽  
Dermal Injection

Cancer caused uncontrolled cell proliferation and triggered by changes on cell information that regulate growth function. Each cell has different potential so many cancer prevalence such as endometrial cancer, lymph node cancer, lung cancer, skin and mammary gland cancer. The aims of this study were to assess the potential of dragon fruit skin ethanolic extract on white rats (Rattus norvegicus) exposed 7,12- Dimethylbenz(á)antrasena (DMBA) on mammary gland based on histopathological features and cyclooxygenase-2 (Cox-2) intensity. This study were used 20 of rats randomly divided into five group and each groups consisted of four rats, i.e: K+ weren’t treated DMBA and extract; K- were treated with DMBA; P1 were treated with DMBA and extract 10 mg/kg BW; P2 were treated with DMBA and extract 15 mg/kg BW; P3 were treated with DMBA and extract 20 mg/kg BW. The DMBA was given by intra dermal injection during twice a week for five weeks and the extracts with gastric tube everyday till 14 days. The data was analyzed by Anova test and continued with Duncan test. The result showed that the histopathological features were decrease significantly on P3. The variables of Cox-2 intensity were decrease significantly on P1, P2 and P3. Conclusion of this study was ethanol extract of dragon fruit skin can decrease neoplastic indication of mammary gland on white rats (R. norvegicus) based on histopathological features and Cox-2 inhibitors.

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