scholarly journals COMPARATIVE STUDY OF MYCOFLORA, ANTIBACTERIAL ACTIVITY AND PHYTOCHEMISTRY OF SELECTED FRESH AND STORED MEDICINAL FRUITS

2017 ◽  
Vol 9 (10) ◽  
pp. 43
Author(s):  
Sushma K. S. ◽  
Puttaswamy Rajeshwari ◽  
Koteshwar Anandrao Raveesha
Keyword(s):  
Diversity Index ◽  
Wiener Index ◽  
Diversity Indices ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Fungal Contamination ◽  
Terminalia Bellerica ◽  
Significant Difference ◽  
Proper Training ◽  
Antibacterial Potential

Objective: To understand the mycoflora, phytochemical constituents and antibacterial potential of fresh and stored herbal fruits of selected plants, extensively used in herbal medicines.Methods: Mycoflora analysis of Terminalia bellerica, Phyllanthus emblica and Myristica fragrans fruits was done employing serial dilution method, colony forming unit (CFU) and relative density of each fungi was recorded. The diversity of fungi associated with test fruits was calculated using various diversity indices. Methanol extracts of test fruits was subjected to preliminary phytochemical analysis, presence or absence of alkaloids, flavonoids, tannins, saponins, terpenoids, quinones and cardiac glycosides was detected. Antibacterial potential of test fruits was studied by well diffusion method.Results: Fresh fruits were free of fungal contamination, whereas stored fruits were found in association with various fungi. A total of 64 isolates of 29 species belonging to 13 genera were identified from stored fruits. Aspergillus was predominant followed by Penicillium. T. bellerica was highly contaminated (1x105CFU/g). Stored fruits of M. fragrans recorded high fungal diversity with highest Simpson’s diversity index (D-1=0.840) and Shannon-Wiener index (H=2.888). P. emblica exhibited high species richness with high Margalef Richness (R=2.925), Menhinick index (E=1.830) and Berger Parker Dominance (d’=0.418). Phytochemical analysis of fresh and stored fruits did not show a significant difference in the presence or absence of tested phyto-constituents. Stored fruit extract recorded up to 41% increase in antibacterial activity.Conclusion: Results suggests the need of proper training to the herbal material handler’s right from the harvest to retail selling, and also points out the need to assess the fungal contamination of herbal materials before using them for herbal drug manufacture.

scholarly journals FLORISTIC STUDY ON ANGIOSPERMS SURROUNDING THE MEDAVAKKAM LAKE, CHENGALPATTU DISTRICT, TAMIL NADU, INDIA

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Amzad Basha Kolar ◽  
Palanivel. S, ◽  
M. Sheik Noor Mohamed ◽  
S. Sheik Mohamed ◽  
M. Shareef Khan ◽  
...  
Keyword(s):  
Native Species ◽  
Tamil Nadu ◽  
Diversity Index ◽  
Species Abundance ◽  
Wiener Index ◽  
Diversity Indices ◽  
Economic Importance ◽  
The Family ◽  
Species Diversity Indices

Biodiversity plays a significant role in maintaining the ecosystem for their sustainable utilization. A preliminary survey was conducted to document the diversity and economic importance of the angiosperms around the Medavakkam Lake, Chengalpattu district, Tamil Nadu. The study area act as a seed bank for native species and provide suitable conditions to endemic and certain endangered species. Diversity indices of the study area was found significant, in which the species abundance, richness and evenness were found. A total of 71 species belonging to 36 families of angiosperms were documented. Among which the Dicotyledons plants were 87.32% and monocotyledon plants were 12.67% of total flora reported. The study area was dominated by the family fabaceae (14.1%) with ten species. Habit wise classification of plants reveal that trees are predominant with 32%, followed by hers (25%), Shrubs (21%), Climbers (13%), undershrubs (6%) and grasses (3%). The Simpson Diversity Index, Shannon Wiener Index and evenness index of the study area is 0.777, 1.5634 and 0.8725 respectively. These plants was found with enormous benefits to the mankind such as medicinal, edible, timber, ornamental, oil and other sources. This investigation divulges that the Medavakkam Lake has substantial angiosperm diversity served as major source for native plants having greater economic importance.

Antibacterial Effects of Calotropisgigantea (L) Extracts on Porphyromonasgingivalis

2020 ◽  
Vol 48 ◽  
pp. 111-118
Author(s):  
Zulfan M. Alibasyah ◽  
D.S. Ningsih ◽  
Sunnati ◽  
R. Andayani ◽  
N.P. Ranggaswuni
Keyword(s):  
Ethanol Extract ◽  
Dilution Method ◽  
Test Results ◽  
Treatment Groups ◽  
Significant Difference ◽  
The One ◽  
Dominant Bacteria ◽  
Post Hoc ◽  
Potential Test ◽  
Antibacterial Potential

The Porphyromonasgingivalis is one of the dominant bacteria that causes chronic periodontitis. These bacteria can penetrate the gingiva and cause tissue damage directly or indirectly with the induction of inflammation. The growth of Porphyromonasgingivalis can be inhibited by administrating the active compounds from plants that have an antibacterial effect such as Calotropisgigantea leaves.This study aimed to determine the antibacterial potential of Bidurileaves (Calotropisgigantea) extract against the Porphyromonasgingivalis ATCC. In this study the Biduri leaves were extracted by maceration method using 70% ethanol solvent. The antibacterial potential test of 70% ethanol extract of Biduri leaves (with a concentration of 5%, 10%, 15%, 20%, 25%, 30%, and 35%) against thePorphyromonasgingivalis was carried out using dilution method with 4 times repetition on Tryptone Soy Agar (TSA) media.The results of the study were analyzed using the One Way ANOVA test showing there were significant differences in the number of Porphyromonasgingivalis ATCC 33277 colonies due to administration of 70% ethanol extract of Bidurileaves (Calotropisgigantea) with p values <0,05. The test results were continued with the Post Hoc Tamhane test to determine the significant difference of the treatment groups. Based on the results of the study it could be concluded that 70% ethanol extract of Biduri leaves (Calotropisgigantea) had antibacterial potential against Porphyromonasgingivalis ATCC 33277 starting from the smallest concentration of 5% to the highest concentration of 35%.

scholarly journals Exotic Tree Species Outnumbering Native Tree Species: an Alarming Situation for the Sustainable Urban Plantation Development

2021 ◽  
Author(s):  
Muhammad Umair Umair Shafique ◽  
Adnan Younis ◽  
Muhammad Asif ◽  
Mansoor Hameed
Keyword(s):  
Tree Species ◽  
Diversity Index ◽  
Wiener Index ◽  
Diversity Indices ◽  
Tree Distribution ◽  
Tree Population ◽  
Exotic Tree ◽  
Native Tree ◽  
Native Tree Species ◽  
The City

Abstract This present study mainly focuses on the tree distribution and diversity of some selected roadsides in Faisalabad, a major city in Punjab, Pakistan. A total census of the tree population on selected roads was carried out and then studied for various parameters. Shannon–Wiener index, Reciprocal of Simpson’s diversity index, and Shannon Evenness were among the different species diversity indices which were studied. Observational results showed that the selected roads in the city hold low tree diversity, with nearly 2858 trees of 34 species, 30 genera, and 15 families. Mainly exotic tree species dominated the plantation of selected roadsides. The topmost three species contributing approximately 60% of the tree population are Conocarpus erectus, Eucalyptus crebra, and Terminalia mantaly. Despite the dominance of exotic tree species, many native tree species are also growing along selected roadsides. Since, Faisalabad has been reported as one of the most polluted cities in the world due to high suspended particulate matter, appropriate efforts are needed to improve the plantation pattern and also to enhance the canopy of the trees in the city to minimize the problem of air pollution.

scholarly journals Phytochemical Analysis and Antibacterial Activity of Purple Leaf Extract [Graptophyllum pictum (L.) Griff] Against Streptococcus mutans

2021 ◽  
Vol 55 (8) ◽  
Author(s):  
Devi Eka Juniarti ◽  
Tuti Kusumaningsih ◽  
Wisnu Setyari Juliastuti ◽  
Adioro Soetojo ◽  
Nabilla Dara Wungsu
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Petri Dish ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Microbiological Analysis ◽  
Significant Difference ◽  
The Relationship ◽  
Purple Leaf

Background. Streptococcus mutans is the leading cause of dental caries. One of many medicinal plants, purple leaf [Graptophyllum pictum (L.) Griff], which contains flavonoids, alkaloids, tannins, steroids, and saponins, is a potential antibacterial agent. Objective. This study aimed to determine the antibacterial activity of purple leaf extract (Graptophyllum pictum L. Griff) against Streptococcus mutans. Methods. Streptococcus mutans were suspended in several Graptophyllum pictum (L.) Griff extract concentrations in a BHIB medium using the dilution method so that the concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78% were obtained. Each tube was incubated for 24 hours, then subcultured in a Tryptone Yeast Extract Cystine medium in a petri dish using a spreader. Each petri dish was set for 24 hours; the growth of the colony, using CFU/mL unit, was manually calculated. The samples were then subjected to microbiological analysis. The Tukey's Honest Significant Difference test was performed to determine if the relationship between the sets of data in the treatment group is statistically significant (p<0.05). Results. Purple leaf extract contains bioactive compounds such as flavonoid, alkaloid, tannin, triterpenoid/ steroid, and saponin. The Minimum Inhibitory Concentration (MIC) of Graptophyllum pictum (L.) Griff against Streptococcus mutans was in concentration 3.125%, and the Minimum Bactericidal Concentration (MBC) was in concentration 6.25%. Conclusion. Purple Leaf Extract [Graptophyllum pictum (L.) Griff] has antibacterial activity against Streptococcus  mutans.

scholarly journals Diversity, distribution and relative abundance of the mosquito fauna (Diptera: Culicidae) of Malakand and Dir Lower, Pakistan

2023 ◽  
Vol 83 ◽  
Author(s):  
M. Attaullah ◽  
S. Gul ◽  
D. Bibi ◽  
A. Andaleeb ◽  
I. Ilahi ◽  
...  
Keyword(s):  
Biological Sciences ◽  
Relative Abundance ◽  
Wiener Index ◽  
Diversity Indices ◽  
Significant Difference ◽  
Chi Squared ◽  
Freshwater Bodies ◽  
Veterinary Entomology ◽  
Abundance And Distribution ◽  
Satellite Species

Abstract The present study was conducted to evaluate the diversity, distribution (C) and relative abundance (RA) of the mosquito fauna (Diptera: Culicidae) of Malakand and Dir Lower, Pakistan. Collection of specimens (n = 1087) was made during September 2018 to July 2019 at six different habitats including freshwater bodies, rice fields, animal sheds, indoors, drains and sewage waters. Specimens were collected through light traps, pyrethrum spray, aspirators and nets and subsequently killed, preserved and then arranged in entomological boxes for identification. Three genera were identified namely Culex, Anopheles and Aedes. A total of fourteen species were identified namely: Cx. quinquefasciatus (Say, 1823), An. stephensi (Liston, 1901), Cx. tritaeniorhynchus (Giles, 1901), Ae. vittatus (Bigot, 1861), An. maculatus (Theobald, 1901), An. fluviatilis (James, 1902), Cx. vishnui (Theobald, 1901), Ae. aegypti (Linnaeus, 1762) An. subpictus (Grassi, 1899), An. dthali (Patton, 1905), An. culicifascies (Giles, 1901), An. pallidus (Theobald, 1901), Ae. albopictus (Skuse, 1894) and An. annularis (van der Wulp, 1884). Cx. quinquefasciatus was found constantly distributed in the study area with RA = 16.5% and C = 100%. An. annularis was found as a satellite species, sporadically distributed in the study area having RA = 0.9% and C = 17%. Diversity indices of mosquitoes in the studied habitats were found as, Shannon-Wiener Index (2.415), Simpson Index (9.919), Fisher’s Index (2.269) and Margalef’s Index (1.859). A statistically significant difference was recorded in mosquito diversity in the six habitats (Kruskal-Wallis, chi-squared, H = 17.5, df = 5, P = 0.003 at α = 0.05). The present study encompasses mosquito fauna of Malakand, Pakistan with respect to diversity, relative abundance and distribution in diverse habitats and all seasons of the year. This will assist scientists working in various fields related with epidemiology, medical and veterinary entomology, ecology and allied areas of biological sciences.

scholarly journals EVALUATION OF ANTIBACTERIAL POTENTIAL OF LEAF AND LEAF DERIVED CALLUS EXTRACTS OF ORTHOSIPHON ARISTATUS (BLUME) MIQ.

2017 ◽  
Vol 10 (5) ◽  
pp. 245
Author(s):  
Nissar Ahmad Reshi
Keyword(s):  
Callus Formation ◽  
Diffusion Method ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Antibacterial Efficacy ◽  
Zone Of Inhibition ◽  
Serial Dilution Method ◽  
Leaf Segments ◽  
Mic Value ◽  
Antibacterial Potential

ABSTRACT:                                                                                                                                   Objective: To evaluate the antibacterial efficacy of chloroform, petroleum ether, ethyl acetate, methanol, ethanol and aqueous extracts of leaf and leaf derived callus of Orthosiphon aristatus against Bacillus cereus, Bacillus subtiltis, Staphylococcus aureus, Streptococcus pyogenes, Enterobater aerogenes, Escherichia coli, Proteus mirabilis, and Klebseilla pneumoniae. Methods: The leaf segments were cut into small pieces of size 1-2 sq. cm and were cultured on Murashige and Skoog solid medium supplemented with different auxins alone and in combination. Antibacterial efficacy was performed by disc diffusion method followed my MIC determination by two fold serial dilution method. Leaf and leaf callus extracts were subjected to the qualitative phytochemical analysis. Results: Maximum callus formation percentage was obtained from the leaf segments cultured on MS medium supplemented with 2, 4-D (2 mg/l). Ethanolic leaf extract showed maximum inhibition activity with 28 mm zone of inhibition against P. mirabilis with MIC value of 0.32 mg/ml. Out of the callus extracts, ethanolic callus extract showed the maximum bio-efficacy against S. aureus with 26 mm zone of inhibition and MIC value of 0.64 mg/ml. Results revealed that both leaf and leaf derived callus are effective against Gram positive and Gram negative test bacteria.Conclusion: The bioefficacy study confirms the strong antibacterial potential of leaf and leaf derived callus of O. aristatus.Keywords: Leaf, Leaf callus, Antibacterial efficacy, Orthosiphon aristatus.

scholarly journals The Influence of Abattoir Wastes on Macroinvertebrate Distribution in River Idemili, Obosi, South-Eastern Nigeria

2017 ◽  
Vol 9 (4) ◽  
pp. 480-486
Author(s):  
Keziah N. IBEMENUGA ◽  
Blessing C. NDUBA ◽  
Joseph E. EYO
Keyword(s):  
Water Quality ◽  
Species Richness ◽  
Diversity Index ◽  
Oxygen Demand ◽  
Wiener Index ◽  
Organic Pollution ◽  
Temperature Conductivity ◽  
South Eastern ◽  
Significant Difference ◽  
Impacted Site

The influence of abattoir wastes on macroinvertebrates distribution in River Idemili, South-Eastern Nigeria, was studied. Three sampling stations 1, 2 and 3 representing upstream of the impacted site, impacted site and downstream of the impacted site respectively were selected. The water quality characteristics, which were significantly different (p<0.05) among the study stations, include water temperature, conductivity, dissolved oxygen and biochemical oxygen demand and phosphatephosphorus. There was no significant difference (p>0.05) in the levels of pH and nitrate-nitrogen among the study stations. Thirteen taxa composed of 201 individuals were encountered. Station 1 had the highest taxa (13) species, as well as the number of individuals (92, 45.8%), followed by station 3 with 12 species and 64 (31.8%) individuals. Station 2 had the least diversity and abundance of 6 species composed of 45 (22.4%) individuals. Macroinvertebrate abundance in the study stations were significantly different (p<0.05). Arthropoda (54.7%) was the dominant phylum. Chironomids (Family: Chironomidae) dominated the samples at all the study stations. Coleoptera was completely absent at the impacted site due to organic pollution. Margalef’s species richness and Shannon Wiener index for general diversity were the highest in stations 1 and 3. The low macroinvertebrate abundance, species richness and Shannon diversity index in station 2 may be attributed to the decimating impacts of abattoir wastes entering the water in this station. Renkonen’s number calculated for pairs of stations revealed that stations 1 and 3 were significantly similar, while stations 1 and 2, 2 and 3 were dissimilar. Conclusively, abattoir wastes distort water quality and affect the community structure, composition, distribution and abundance of macroinvertebrates inhabiting aquatic ecosystems.

scholarly journals Floristic composition and distribution pattern of herbaceous plant diversity in fallow lands of the central districts of Punjab, India

2020 ◽  
Vol 12 (8) ◽  
pp. 15864-15880
Author(s):  
Jashanpreet Kaur ◽  
Rajni Sharma ◽  
Pushp Sharma
Keyword(s):  
Plant Species ◽  
Plant Diversity ◽  
Diversity Index ◽  
Similarity Index ◽  
Floristic Composition ◽  
Wiener Index ◽  
Diversity Indices ◽  
Herbaceous Plant ◽  
Dissimilarity Index ◽  
Fallow Lands

This study explores the change in composition of herbaceous plants with change in season and site in the fallow lands of central districts of Punjab, India.  Overall 41 plant species were reported from studied sites.  Poaceae and Asteraceae were recorded as dominant families with seven and six plant species, respectively.  Density and IVI values of perennial plant species were recorded to be the maximum from July to September and for annuals maximum values were from February to March and from July to September.  Diversity indices like Shannon Wiener index, evenness index, Menhinick index, and Simpson diversity index values showed variation with season and site.  Similarity index value between studied sites was recorded to be the minimum in July (0.45) which indicates a maximum value of dissimilarity index in this month.  The information generated in this study can be exploited by researchers for conservation of natural plant diversity and timely assessments of such areas help to study climate change. ,,,,,.

Surface treatment with silver particles isles on Titanium cp: study of antimicrobial activity

2020 ◽  
Vol 9 (4) ◽  
pp. e27942662
Author(s):  
Patrícia Capellato ◽  
Cláudia Eliana Bruno Marino ◽  
Gilbert Silva ◽  
Lucas Victor Benjamim Vasconcelos ◽  
Rodrigo Perito Cardoso ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Surface Treatment ◽  
Titanium Surface ◽  
Silver Clusters ◽  
Silver Particles ◽  
Silver Deposition ◽  
Dispersed Particles ◽  
Significant Difference ◽  
Diffusion Assay ◽  
Antibacterial Potential

During the last decades, researchers have been growing the interest in surface treatment with an antimicrobial agent. Silver nanoparticles (AgNPs) are widely used in biomedical fields due to their potent antimicrobial activity. So, in this study was investigated silver particles (isles) coated on titanium surface for dental and orthopedic application. Silver particles coating process on titanium surface were performed via sputtering that is a plasma-assisted deposition technique with and titanium without treatment was applied as comparing standard. Plasma treatment parameters were optimized so that the result was not a thin film of Ag but dispersed particles of Ag on the Ti-cp surface. The alloy surfaces were investigated using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). In order to investigate antibacterial potential Staphylococcus aureus and Escherichia coli have been used at Agar diffusion assay. The results were analyzed by analysis of variance (ANOVA) in order to verify significant difference antimicrobial activity between samples that have shown no difference between the surfaces studied treatments. For silver deposition scattered particles (isles) over titanium surface for a 10-minute treatment, EDS revealed by silver clusters that the particles were not properly scattered onto surface, hence, the low effectiveness in antibacterial activity.

Assemblage Patterns of Hymenoptera at Different Elevations of Gunung Datuk, Rembau

2017 ◽  
Vol 14 (2) ◽  
pp. 1 ◽  
Author(s):  
Noor Nasuha Abd Aziz ◽  
Siti Khairiyah Mohd Hatta ◽  
Idris Abd Ghani ◽  
Saiyid Jalaluddin Saiyid Shaifuddin
Keyword(s):  
Species Richness ◽  
Diversity Index ◽  
High Elevation ◽  
Significant Difference ◽  
Index Site ◽  
Evenness Index ◽  
Abundance And Diversity ◽  
Richness Index ◽  
Paired T Test ◽  
Malaise Traps

A study on abundance and diversity of Hymenoptera was conducted in Gunung Datuk, Rembau. Samplings were conducted from November 2014 to February 2015 using six Malaise traps. Three traps were placed at Site 1 at 700m height for high elevation and the remaining traps were placed at Site 2 at 200m height for low elevation. A total number of 221 Hymenopteran were collected which consist of nine families namely Ichneumonidae, Formicidae, Braconidae, Bethylidae, Evaniidae, Tiphiidae, Vespidae, Pompilidae and Apidae. In this study, 93 individuals were obtained from Site 1, comprising nine families and 43 morphospecies while 127 individuals were obtained from Site 2 with nine families and 45 morphospecies. Formicidae was the most dominant family collected from both sites with a total of 104 individuals while the least family recorded was Apidae with only one individual. Shannon’s Weiner Diversity Index (H’) showed Site 1 had the higher diversity value with H’ = 3.17 compared to Site 2 with value H’ = 3.12. For Evenness Index, Site 1 had higher value compared to Site 2 with E’ = 0.84 and E’ = 0.82 respectively. Moreover, for Margalef Richness Index, Site 1 recorded R’ = 9.24 while site two recorded R’ = 9.08 which concluded that Site 1 had higher species richness compared to Site 2. Paired t-test showed that both sites had no significant difference with p>0.05. Overall study showed that the diversity and abundance of Hymenoptera in Gunung Datuk were low since the value of H’ is less than 3.50.

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