scholarly journals ANTIBACTERIAL EFFECTIVENESS OF SELECTED MOROCCAN ESSENTIAL OILS AGAINST THE HIGHLY VIRULENT JP2 CLONE OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS

2017 ◽  
Vol 9 (2) ◽  
pp. 47
Author(s):  
Leila Lakhdar ◽  
Abdellah Farah ◽  
Idriss Lahlou ◽  
Sana Rida ◽  
Amal Bouziane ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Aggressive Periodontitis ◽  
Oral Bacteria ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Broth Microdilution Method ◽  
Origanum Compactum ◽  
Highly Virulent

<p><strong>Objective: </strong><em>Aggregatibacter actinomycetemcomitans </em>(<em>Aa</em>) serotype b JP2 clone is a highly virulent strain, considered as a major etiologic agent in aggressive periodontitis in patients of African descent, such as Moroccan adolescents. Antibiotics have been and continue to be the only effective treatment of periodontal infections caused by this periodontal bacterium. However, today there is enough scientific evidence on the existence of an increased resistance of oral bacteria to antibiotics. Therefore, the search for new natural agents, that are safe and effective, such "essential oils," has become a necessity. The present study was conducted to evaluate the <em>in vitro</em> antibacterial activities of three selected essential oils from Moroccan aromatic medicinal plants (<em>Origanum compactum</em>, <em>Thymus vulgaris</em> and <em>Cymbopogon martinii</em>) against clinical Moroccan isolate of <em>Aa </em>JP2 strain.</p><p><strong>Methods: </strong>Antibacterial activity of essential oils was investigated using agar well diffusion method, then measured using broth microdilution method.</p><p><strong>Results: </strong>All the selected essential oils exhibited significant antibacterial activity on the highly pathogenic JP2 strain of <em>Aa</em>. Essential oil of <em>Origanum compactum</em> was found to be the most effective with a minimum inhibitory concentration (MIC) value of 0.03% (v/v) and a minimum bactericidal concentration value (MBC) of 0.07%.</p><p><strong>Conclusion: </strong>The present findings indicate the possibility of exploiting these essential oils as potential antimicrobial agents in treatment of aggressive periodontitis associated to this pathogen.</p>

scholarly journals In VitroAntibacterial Activity of Essential Oils againstStreptococcus pyogenes

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Julien Sfeir ◽  
Corinne Lefrançois ◽  
Dominique Baudoux ◽  
Séverine Derbré ◽  
Patricia Licznar
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Dilution Method ◽  
Origanum Compactum ◽  
Broth Dilution

Streptococcus pyogenesplays an important role in the pathogenesis of tonsillitis. The present study was conducted to evaluate thein vitroantibacterial activities of 18 essential oils chemotypes from aromatic medicinal plants againstS. pyogenes. Antibacterial activity of essential oils was investigated using disc diffusion method. Minimum Inhibitory Concentration of essential oils showing an important antibacterial activity was measured using broth dilution method. Out of 18 essential oils tested, 14 showed antibacterial activity againstS. pyogenes. Among themCinnamomum verum, Cymbopogon citratus, Thymus vulgaris CT thymol, Origanum compactum, andSatureja montanaessential oils exhibited significant antibacterial activity. Thein vitroresults reported here suggest that, for patients suffering from bacterial throat infections, if aromatherapy is used, these essential oils, considered as potential antimicrobial agents, should be preferred.

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oils from the Algerian Endemic Origanum glandulosum Desf. against Multidrug-Resistant Uropathogenic E. coli Isolates

Antibiotics ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 29 ◽  
Author(s):  
Larbi Zakaria Nabti ◽  
Farida Sahli ◽  
Hocine Laouar ◽  
Ahmed Olowo-okere ◽  
Joice Guileine Nkuimi Wandjou ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
E Coli ◽  
Broth Microdilution Method ◽  
Resistant Strains ◽  
Tract Infections

Antibiotics are becoming ineffective against resistant bacteria. The use of essential oils (EOs) may constitute an alternative solution to fight against multidrug-resistant bacteria. This study aims to determine the chemical composition of EOs from five populations of the endemic Algerian Origanum glandulosum Desf. and to investigate their potential antibacterial activity against multidrug-resistant uropathogenic E. coli strains. The EOs were obtained by hydrodistillation and their composition was investigated by gas chromatography/mass spectrometry (GC/MS). The antibacterial activity was evaluated by the disc diffusion method against eight E. coli strains (six uropathogenic resistant and two referenced susceptible strains). Minimum inhibitory and bactericidal concentrations (MIC/MBC) were obtained by the broth microdilution method. The main EO components were thymol (15.2–56.4%), carvacrol (2.8–59.6%), γ-terpinene (9.9–21.8%) and p-cymene (8.5–13.9%). The antibacterial tests showed that all the EOs were active against all the strains, including the multidrug-resistant strains. The EO from the Bordj location, which contained the highest amount of carvacrol (59.6%), showed the highest antibacterial activity (inhibition diameters from 12 to 24.5 mm at a dilution of 1/10). To our knowledge, this is the first description of the activity of O. glandulosum EOs against resistant uropathogenic strains. Our study suggests that O. glandulosum EO could be used in some clinical situations to treat or prevent infections (e.g., urinary tract infections) with multidrug-resistant strains.

scholarly journals Influence of Some Antibiotics and Essential Oils Used Alone or in Combination on the Vitality of Presumptive Probiotic Lactic acid Bacteria

2021 ◽  
pp. 1-14
Author(s):  
Gislaine Aurelie Kemegne ◽  
Aurore Vanessa Nana Ngahmi ◽  
Abraham Nkoue Tong ◽  
Sylvain Leroy Sado Kamdem ◽  
Jean Justin Essia Ngang
Keyword(s):  
Antibacterial Activity ◽  
Lactic Acid ◽  
Chemical Composition ◽  
Lactic Acid Bacteria ◽  
Essential Oils ◽  
Experimental Studies ◽  
Thymus Vulgaris ◽  
Probiotic Lactic Acid Bacteria ◽  
Origanum Compactum ◽  
The One

Aims: The aim of this study was to assess the in vitro antibacterial activity of selected antibiotics and essential oils alone or in combination, on selected presumptive probiotic lactic acid bacteria. Study Design: Experimental studies. Place and Duration of Study: Department of Microbiology of the University of Yaounde I between August 2017 and December 2017 (5 months). Methodology: The chemical composition of five essential oils was determined by gas chromatography coupled with Solid-phase micro extraction. Then the sensitivity of four lactic acid bacteria to the essential oils and four antibiotics was assessed by the well diffusion and macrodilution method. Subsequently, two essential oils active on these bacteria and broad spectrum antibiotics were combined according to the central composite design plan. Results: In general, the chemical composition of essential oils is very diverse, with the example of carvacrol found only in Origanum compactum at 53.24% and thymol in Thymus vulgaris at 56.19% and in Origanum compactum at 15.28%. The antibacterial activity shows that the majority of antibiotics used are active on the bacteria in the study compared to the essential oils where two were active (Origanum compactum and Cymbopogon winterianus). The evaluation of the combinations of essential oils and antibiotics in terms of kinetics has given us three cases: the first case is the one with no acidity or no growth at all; the second is the one where growth is normal; the third where growth is delayed with a more pronounced latency phase. Conclusion: This study suggest that the effect of essential oils and medicinal plant used alone or in combination to antibiotics on the gut microbiota have to be evaluated for validation as well as their toxicity activities before using them for human therapy.

scholarly journals Antibacterial activity of aromatic plants essential oils from Serbia against the Listeria monocytogenes

2009 ◽  
Vol 54 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Anita Klaus ◽  
Damir Beatovic ◽  
Miomir Niksic ◽  
Slavica Jelacic ◽  
Tanja Petrovic
Keyword(s):  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Disk Diffusion Method ◽  
Growth And Survival ◽  
Salvia Officinalis L ◽  
Rosmarinus Officinalis L

The purpose of this study was to examine the effectiveness of selected essential oils for the control of the growth and survival of pathogenic bacteria Listeria monocytogenes ATCC 19112 and Listeria monocytogenes ATCC 19115, which are of significant importance in food hygiene. Essential oils extracted from Salvia officinalis L., Rosmarinus officinalis L., Majorana hortensis Moench., Thymus vulgaris L., Carum carvi L., Pimpinella anisum L. and Coriandrum sativum L. were evaluated. Antibacterial activity was done by the disk diffusion method in the presence of pure essential oils and four suspensions in alcohol. The best results obtained with Thymus vulgaris and Majorana hortensis essential oils, which were acting microbicidaly on both observed strains of Listeria monocytogenes, even in the small concentration. Because some of the essential oils were highly inhibitory even in small quantities to selected pathogenic bacteria, they may provide alternatives to conventional antimicrobial additives in foods. .

scholarly journals Evaluation of the in vitro antibacterial activity of some essential oils and their blends against Staphylococcus spp. isolated from episodes of sheep mastitis

2021 ◽  
Author(s):  
Filippo Fratini ◽  
Margherita Giusti ◽  
Simone Mancini ◽  
Francesca Pisseri ◽  
Basma Najar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Diffusion Method ◽  
Minimal Bactericidal Concentration ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Method ◽  
In Vitro Antibacterial Activity ◽  
High Concentrations ◽  
Thymus Serpyllum

AbstractStaphylococcus aureus and coagulase-negative staphylococci are among the major causes of mastitis in sheep. The main goal of this research was to determine the in vitro antibacterial activity of several essential oils (EOs, n 30), then five of them were chosen and tested alone and in blends against staphylococci isolates. Five bacteria were isolated from episodes of ovine mastitis (two S. aureus and three S. xylosus). Biochemical and molecular methods were employed to identify the isolates and disk diffusion method was performed to determine their antimicrobial-resistance profile. The relative percentage of the main constituents in the tested essential oils and their blends was detected by GC-EIMS analysis. Antibacterial and bactericidal effectiveness of essential oils and blends were evaluated through minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). All of them showed sensitivity to the used antimicrobials. The EOs with the highest antibacterial activity were those belonging to the Lamiaceae family characterized by high concentrations of thymol, carvacrol and its precursor p-cymene, together with cinnamon EO, rich in cinnamaldehyde. In terms of both MIC and MBC values, the blend composed by Thymus capitatus EO 40%, Cinnamomum zeylanicum EO 20%, Thymus serpyllum EO 20% and Satureja montana EO 20% was found to be the most effective against all the isolates. Some essential oils appear to represent, at least in vitro, a valid tool against ovine mastitis pathogens. Some blends showed a remarkable effectiveness than the single oils, highlighting a synergistic effect in relation to the phytocomplex.

scholarly journals Anti-bacterial activity of three essential oils - An in vitro study

2019 ◽  
Vol 10 (2) ◽  
pp. 1049-1053 ◽  
Author(s):  
Geetha RV ◽  
John Rozar Raj B ◽  
Lakshmi Thangavelu
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Bacterial Species ◽  
In Vitro Study ◽  
Oral Bacteria ◽  
Zone Of Inhibition ◽  
Thyme Oil ◽  
Rosemary Oil

To conduct a study regarding the antibacterial activity of essential oils against bacteria causing Caries. Essential oils are distillates of the volatile compounds of a plant’s secondary metabolism and may act as photoprotective agents. Their curative effect has been known since antiquity. It is based on a variety of pharmacological properties which are specific for each plant species. The mouth contains a variety of oral bacteria, but only a few species of bacteria are believed to cause dental caries. Antibacterial activity of the three essential oils, Rosemary oil, Holy basil oil, Thyme oil was screened against Streptococcus mutans, using disc diffusion technique. The rosemary oil was more effective against Streptococcus mutans with a zone of inhibition of 52 mm diameter (at concentration 200 µl), Rosemary oil showed a zone of inhibition of 44 mm diameter and with thyme oil, the zone diameter was 30 mm. The results of this study showed that the essential oils at different concentrations exhibited antibacterial activity against the bacterial species tested.

scholarly journals CHEMICAL COMPOSITION OF ESSENTIAL OILS FROM VITEX AGNUS-CASTUS L. GROWING IN MOROCCO AND ITS IN VITRO ANTIBACTERIAL ACTIVITY AGAINST CLINICAL BACTERIA RESPONSIBLE FOR NOSOCOMIAL INFECTIONS

2018 ◽  
Vol 11 (10) ◽  
pp. 365 ◽  
Author(s):  
Fatima El Kamari ◽  
Amal Taroq ◽  
Yassine El Atki ◽  
Imane Aouam ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Nosocomial Infections ◽  
University Hospital ◽  
Mass Spectrometry Analysis ◽  
Diffusion Method ◽  
Strong Activity ◽  
Agnus Castus

Objective: The aim of the current study is to determine the chemical composition and evaluate antibacterial activity of Vitex agnus-castus L. (VAC) essential oils against some bacteria causing nosocomial infections in the neonatal and intensive care rooms at the university hospital center of Fez Morocco. Methods: The phytochemical screening of essential oils was determined using gas chromatography (GC) and GC-mass spectrometry analysis. The antibacterial test was evaluated against Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria species (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis) using disc diffusion method. Results: Twenty-nine components were identified in the fruits’ oil representing 93.1% of total oil. The major components in the fruits oil are 1,8-cineole (11.6%), α-thujene (9.3%), phyllocladene (8.2%), α-pinene (7.9%), caryophyllene (5.9%), and cubenol (5%). Furthermore, 28 components were identified in the leaf essential oil. The main component was caryophyllene (9.5%), followed by 1,8-cineole (8.7%), manoyl oxide (7.3%), eugenyl acetate (7.1%), phyllocladene (6.8%), and α-pinene (5.2%). Antibacterial activity of both oils showed a strong activity against nosocomial bacteria tested. Conclusion: Essential oils of Moroccan VAC could be exploited as natural drugs for bacteria, especially those who have acquired resistance to conventional antibiotics.

scholarly journals In Vitro Activity of Essential Oils Against Planktonic and Biofilm Cells of Extended-Spectrum β-Lactamase (ESBL)/Carbapenamase-Producing Gram-Negative Bacteria Involved in Human Nosocomial Infections

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 272 ◽  
Author(s):  
Ramona Iseppi ◽  
Alessandro Di Cerbo ◽  
Piero Aloisi ◽  
Mattia Manelli ◽  
Veronica Pellesi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Essential Oils ◽  
Disk Diffusion ◽  
Mentha Piperita ◽  
Thymus Vulgaris ◽  
Extended Spectrum ◽  
Disk Diffusion Assay ◽  
Diffusion Assay

The aim of this study was to analyze the antibacterial activity of four essential oils (EOs), Melaleuca alternifolia, Eucalyptus globulus, Mentha piperita, and Thymus vulgaris, in preventing the development and spread of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae, metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa and carbapenemase (KPC)-producing Klebsiella pneumoniae. A total of 60 strains were obtained from the stock collection from the Microbiology Laboratory of Hesperia Hospital, Modena, Italy. Twenty ESBL-producing E. coli, 5 K. pneumoniae, 13 KPC-producing K. pneumoniae, and 20 MBL-producing P. aeruginosa were cultured and reconfirmed as ESBL and carbapenamase producers. Polymerase chain reaction was used for the detection of genes responsible for antibiotic resistance (ESBL and KPC/MBL). Antibacterial activity of the EOs was determined using the agar disk diffusion assay, and minimal inhibitory concentrations (MICs) were also evaluated. Lastly, adhesion capability and biofilm formation on polystyrene and glass surfaces were studied in 24 randomly selected strains. M. alternifolia and T. vulgaris EOs showed the best antibacterial activity against all tested strains and, as revealed by agar disk diffusion assay, M. alternifolia was the most effective, even at low concentrations. This effect was also confirmed by MICs, with values ranging from 0.5 to 16 µg/mL and from 1 to 16 µg/mL, for M. alternifolia and T. vulgaris EOs, respectively. The EOs’ antibacterial activity compared to antibiotics confirmed M. alternifolia EO as the best antibacterial agent. T. vulgaris EO also showed a good antibacterial activity with MICs lower than both reference antibiotics. Lastly, a significant anti-biofilm activity was observed for the two EOs (*P < 0.05 and **P < 0.01 for M. alternifolia and T. vulgaris EOs, respectively). A good antibacterial and anti-biofilm activity of M. alternifolia and T. vulgaris EOs against all selected strains was observed, thus demonstrating a future possible use of these EOs to treat infections caused by ESBL/carbapenemase-producing strains, even in association with antibiotics.

scholarly journals Chemical Composition, Olfactory Analysis and Antibacterial Activity of Thymus vulgaris Chemotypes Geraniol, 4-Thujanol/Terpinen-4-ol, Thymol and Linalool Cultivated in Southern France

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Erich Schmidt ◽  
Jürgen Wanner ◽  
Martina Höferl ◽  
Leopold Jirovetz ◽  
Gerhard Buchbauer ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Thymus Vulgaris ◽  
Gram Positive ◽  
Southern France ◽  
Food Borne ◽  
Brochothrix Thermosphacta ◽  
Olfactory Analysis ◽  
The Impact ◽  
And Staphylococcus Aureus

The essential oils of four chemotypes of Thymus vulgaris L. (Lamiaceae) were analyzed for their composition and antibacterial activity to assess their different properties. GC-MS and GC-FID analyses revealed that the essentials oils can be classified into the chemotypes thymol (41.0% thymol), geraniol (26.4% geraniol), linalool (72.5% linalool) and 4-thujanol/terpinen-4-ol (42.2% cis- and 7.3% trans-sabinene hydrate, 6.5 % terpinen-4-ol). The olfactory examination confirmed the explicit differences between these chemotypes. Furthermore, antibacterial activity was investigated against several strains of two Gram-positive ( Brochothrix thermosphacta and Staphylococcus aureus) and four Gram-negative food-borne bacteria ( Escherichia coli, Salmonella abony, Pseudomonas aeruginosa and P. fragi). All essential oil samples were demonstrated to be highly effective against Gram-positive strains, whereas the impact on Gramnegative microorganisms was significantly smaller, but still considerable. The results obtained indicate that, despite their different properties, the essential oils of selected T. vulgaris chemotypes are potent antimicrobials to be employed as useful additives in food products as well as for therapeutic applications.

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