scholarly journals Physicochemical and Phytochemical Studies on Sphaeranthus indicus Linn. with HPTLC Fingerprinting

2021 ◽  
Vol 11 (2) ◽  
pp. 100-107
Author(s):  
Mohit Garg ◽  
Neelesh Dwivedi
Keyword(s):  
Water Soluble ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Ph Values ◽  
Natural Agents ◽  
Sphaeranthus Indicus ◽  
Health And Healing ◽  
Therapeutic Compounds ◽  
Phytochemical Studies

Ayurveda, the oldest paradigm of health and healing known to the world, grew out of the Rishi tradition. The medical and research community is constantly seeking for new natural agents. Sphaeranthus indicus Linn. (Gorakhmundi, Family: Asteraceae), is widely used in Ayurvedic system of medicine to treat various diseases. The present investigation was designed to perform physicochemical and phytochemical analysis with HPTLC fingerprints of Sphaeranthus indicus (leave, flower, and stem) to establish the standard parameters of this herb. Different analytical parameters like extractive values, total ash, acid insoluble ash and water-soluble ash, moisture content, pH values of different parts of the drug were performed. Preliminary phytochemical screening was done to detect and quantify different active constituents such as alkaloids, flavanoids, proteins and total poly phenol using various analytical methods. Our preliminary data of phytochemical screening of the extracts revealed the presence of steroids, carbohydrates, proteins, terpenoid, flavonoid, tannins, alkaloids, resin and saponin. In the current study we established the HPTLC fingerprint of the extract using phytochemical standards. We believe that our study provides a substantial data for identification, purification, standardization, and phytochemical characterization of novel therapeutic compounds that can be used potential drug to combat various diseases for betterment of mankind. Keywords: Sphaeranthus indicus, Ayurveda, physicochemical, UV- Spectrophotometer, HPTLC

Phytochemical characterization of Caraway (Carum carvi) seed extract and its use as a potent medicinal agent

2021 ◽  
Vol 16 (10) ◽  
pp. 102-110
Author(s):  
Vivek Chauhan ◽  
Sunita Devi ◽  
Sonaxi Sharma ◽  
Shamsher S. Kanwar
Keyword(s):  
Staphylococcus Epidermidis ◽  
Arable Land ◽  
Seed Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Food Industries ◽  
Carum Carvi ◽  
Medicinal Properties ◽  
The Village

Carum carvi (Caraway) is a member of Apiaceae family which originated in Asia. Due to its economic importance, it is widely cultivated. Caraway is the only annual species, commonly present in arable land, moist meadows and on low lands to mountains. Caraway has different applications in pharmaceutical and food industries. Phytochemical screening of different medicinal plants is helpful in identifying new sources of industrially and therapeutically important compounds. In this study, Caraway obtained from forest surrounding the village Goherman, Lahaul and Spiti, Himachal Pradesh (India) was used. Seed extract of caraway extracted in methanol (MSE) and distilled water (WSE) was used for phytochemical analysis to determine the constituents of caraway seeds. Further, caraway seeds extract was checked for antibacterial activity [Staphylococcus aureus (ATCC 6538), Salmonella typhimurium (NCTC 74), Escherichia coli (ATCC 25922) and Staphylococcus epidermidis (ATCC 12228)], antioxidant activity and acid neutralizing activity. After analysing the results, it can be said that caraway seeds possess many medicinal properties and it can be used in enhancing human health.

scholarly journals Pharmacognostical, Physico-Chemical and Preliminary Phytochemical Analysis of Whole Plant of Piper Longum L.

2021 ◽  
Vol 8 (8) ◽  
pp. 440-449
Author(s):  
Fathima Suman P ◽  
Minhaj . ◽  
I Arthi
Keyword(s):  
Water Soluble ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Plant Identification ◽  
Piper Longum ◽  
Whole Plant ◽  
Physico Chemical ◽  
Physicochemical Evaluation ◽  
Physicochemical Constants

This study deals with the Pharmacognostical physicochemical and phytochemical evaluation of the whole plant of Piper longum L. Pharmacognostical studies include macroscopic, microscopic and physicochemical evaluation. Physicochemical constants showed the values of total ash (10.8%), acid insoluble ash (1.3%), water soluble ash (3.2%), sulphated ash (12%), nitrated ash (1.85%), hot extraction (11.2%), water soluble extractive (4.8%), ethanol soluble extractive (12.4%), ether soluble extractive (5.6%), loss on drying (29%), swelling index (0.33) and mucilage content (0.8%) w/w. The preliminary phytochemical screening of the alcoholic extract showed presents of alkaloids, phenolic compounds, tannins, mucilage, starch, steroid and flavonoids. This study helps to development of different standard parameters of the plant which help the plant identification and authentication. Keywords: Piper longum L., Whole plant, Pharmacognostic, Phytochemical, Extract.

scholarly journals PHARMACOGNOSTIC STANDARDIZATION, PRELIMINARY PHYTOCHEMICAL SCREENING AND TLC FINGERPRINTING OF THE BARK OF CASCABELA THEVETIA L.

2019 ◽  
pp. 125-130
Author(s):  
Neelutpal Gogoi ◽  
Biman Bhuyan ◽  
Trinayan Deka
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Cork Cell ◽  
Layer Chromatography

Objectives: In this study, systematic pharmacognostic study and preliminary phytochemical screening of the bark of Cascabela thevetia L. were carried out. Methods: The selected plant part was collected, processed and stored in an airtight container. From the bark different pharmacognostic studies like macroscopic and microscopic evaluation, physicochemical parameters, fluorescence analysis were done. Powdered bark was successively extracted by petroleum ether, chloroform, ethyl acetate, and methanol using a Soxhlet apparatus and finally macerated with the hydro-alcoholic solvent system (30:70). The preliminary phytochemical analysis and thin layer chromatography of the extracts were done to find the nature and number of the different phytoconstituents present. Results: Transverse microscopy reveals the presence of crystal oxalate, cork cell, starch granules, vascular bundle, phloem fiber, parenchyma cells, and collenchyma cells. Powder microscopy also showed the presence of cork cell, fiber and calcium oxalate crystal. Results obtained in different physicochemical analysis like total ash, acid insoluble ash, water soluble ash, alcohol-soluble extractive, water-soluble extractive, and moisture content were 8.67%, 0.83%, 5.33%, 4.53%, 12.27%, and 7.83% respectively. Phytochemical analysis showed the presence of alkaloid, flavonoid, triterpenoid, phytosterol, tannin, saponin, anthraquinone, carbohydrate and fatty acid in the different extracts. TLC (Thin Layer Chromatography) study revealed 4 spots in petroleum ether, chloroform, ethyl acetate, and methanol extracts and 3 spots in the Hydro-alcoholic extract with different solvent systems. Conclusion: The results obtained from the study will provide a reliable basis for identification, purity, and quality of the plant.

scholarly journals PHYSICOCHEMICAL AND PHYTOCHEMICAL ANALYSIS OF Mangifera indica linn.( AAMRA KERNEL )

2020 ◽  
Vol p4 (05) ◽  
pp. 2394-2400
Author(s):  
Pooja Dogra ◽  
A. Rama Murthy ◽  
Sumit Nathani
Keyword(s):  
Mangifera Indica ◽  
Natural Antioxidants ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Tropical Regions ◽  
Potential Source ◽  
Mango Kernel ◽  
Phytochemical Constituents ◽  
Phytochemical Studies

Introduction: Mangifera Indica Linn., Anacardiaceae family, is a large evergreen tree of tropical and sub-tropical regions of the world. Mango kernel can be used as a potential source for functional food ingredi-ents, animal feeds, antimicrobial compounds and cosmetic due to its high quality of fat and protein as well as high levels of natural antioxidants. Aim: The aim of the present study was to evaluate the physicochemical and phytochemical analysis of Mangifera Indica Linn. Methods Aamra Kernel were collected, cleaned, dried and powdered. Aqueous and alcoholic extracts of Aamra kernel were analyzed for physicochemical and phytochemical constituents. The study was conduct-ed as per the guidelines of Ayurvedic pharmacopeia of India. Results: The result of physicochemical parameters (moisture content: 10.2%, total ash: 2.12%, acid insolu-ble ash: 0.3%, water soluble ash: 1.42%, aqueous extractive value: 14.67% and alcoholic extractive value 12.98%). The phytochemical studies revealed the presence of active constituents, carbohydrates, amino acid, steroids, proteins, saponins, alkaloids, glycosides, tannins in aqueous and alcoholic extracts. Conclusion: All studies standardization parameters like physicochemical and phytochemical screening pa-rameters provide the knowledge in the identification, authentication of kernel of Mangifera Indica Linn. Hence it is concluded that this drug supposed to be great beneficial and boon for our society.

scholarly journals PHARMACOGNOSTICAL, PHYSICOCHEMICAL AND PHYTOCHEMICAL STUDIES OF PIPER LONGUM LINN. FRUITS

2019 ◽  
Vol 1 (1) ◽  
pp. 19-24
Author(s):  
Priyanka Sharma
Keyword(s):  
Chemical Constituents ◽  
Volatile Oil ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Chemical Parameters ◽  
Piper Longum ◽  
The Family ◽  
Perennial Shrub ◽  
Physical And Chemical ◽  
Phytochemical Studies

Introduction: Piper longum Linn, belonging to the family Piperaceae, is a climber, perennial shrub which is commonly found in India.  The dried fruits of Piper longum are widely used in Ayurvedic System of medicines since time unknown. It posses significant pharmacological properties due to presence of variety of chemical constituents in it. Objective: The present study is aimed to evaluate the pharmacognostical, physiochemical and phytochemical parameters for Piper longum fruits, as per the WHO guidelines for herbal drug standardization. Materials and Methods: WHO emphasized the use of standardized herbs and formulations for safety and best therapeutic results. Results and Discussion: Pharmacognostical studies shows that the fruits of Piper longum are found as green when fresh which turns grayish black upon drying. It posses pungent, bitter and acrid taste. The fruits are cylindrical with small petiole. The powder microscopy shows the presence of brown content, oleo resins, stone cells and calcium oxalate crystals. Physiochemical analysis shows variable extractive values in different solvents with maximum extractives of 20.6±0.021% in water and minimum extractive value of 6.6±0.036% in chloroform. Total Ash value of 8.3±0.015%, Acid Insoluble Ash- 1.16±0.025% and Water Soluble Ash- 5.5±0.01%, Foreign Matter- 1.62±0.12%, Moisture content of 15.70±0.051%, Bitterness value-1.96, Swelling Index-1.5±0.01 and Foaming Index- 102.33. Phytochemical analysis of the fruits of Piper longum shows the presence of alkaloids, volatile oil, tannins, fixed oils, and phenolics. Conclusion: The botanical, physical and chemical parameters obtained in this study can be used for establishing the identity and purity of the drug that will lead to safety and efficacy of the herb.

scholarly journals Identification of the Secondary Metabolites and Characterization of Lagerstroemia Loudonii T. & B.

2021 ◽  
Vol 6 (1) ◽  
pp. 1-6
Author(s):  
Fahrauk Faramayuda ◽  
Faizal Hermanto ◽  
Ari Sri Windyaswari ◽  
Soraya Riyanti ◽  
Viola Aditya Nurhayati
Keyword(s):  
Herbal Medicine ◽  
Specific Gravity ◽  
Traditional Medicine ◽  
Ash Content ◽  
Water Soluble ◽  
Raw Material ◽  
Bark Extract ◽  
Phytochemical Screening

Bungur  (Lagerstroemia loudonii T. B) is a type of plant widely grown in Indonesia and can be found in teak forests, mixed forests, and is found as ornamental plants or protective trees on the roadside. In the fruit section, Lagerstroemia loudonii is used as antituberculous and antimalarial. On the bark, the part is used as antidiarrheal. Based on some parts of the Lagerstroemia loudonii  plants' activity data, this plant has the potential to be developed into traditional medicine. Standardized traditional medicine material is necessary to identify efficacious compounds and characterization in some parts of Lagerstroemia loudonii. The purpose of this research is to develop Lagerstroemia loudonii into traditional herbal medicine or standardized herbal medicine. Identification of efficacious compounds and characterization of crude leaf drugs, bark, stems, and fruit of Lagerstroemia loudonii. The phytochemical screening phase of the crude drugs of leaves, bark, stems, and fruit ofLagerstroemia loudonii against includes examining alkaloids, flavonoids,  quinones, tannins, polyphenols, saponins, steroids and triterpenes, monoterpenoids and sesquiterpenoids. The determination of the characteristics of raw material carried out includes nonspecific parameters. Nonspecific parameters are the determination of total ash content, water-soluble ash content, acid insoluble ash content. each experiment was carried out three times and calculated the average yield and deviation.  Identification results of the class of efficacious compounds in some parts of the Lagerstroemia loudonii  plant are on the leaves and fruits containing alkaloids, flavonoids, saponins, quinones, tannins, polyphenols, monoterpenoids, and sesquiterpenoids as well as steroids and triterpenoids. At the bark and stem, the bark contains alkaloids, flavonoids, saponins, quinones, tannins, polyphenols, monoterpenoids, and sesquiterpenoids. Characterization results of Lagerstroemia loudonii  leaf extract total ash content 4.45 ± 0.30% w/w, water-soluble ash content 4.08 ± 0.27% w/w, acid insoluble ash content 0.59 ± 0.06% w/w, the extract specific gravity was 0.59 ± 0.063. Lagerstroemia loudonii  stem bark extract, total ash content 1.94 ± 0.12% w/w, water-soluble ash content 1.47 ± 0.03% w/w, acid insoluble ash content 0.24 ± 0.02% w/w, the extract specific gravity is 0.82 ± 0.01. Lagerstroemia loudonii  stem extract, total ash content3.18 ± 0.16% w/w, water-soluble ash content 2.36 ± 0.38% w/w, acid insoluble ash content 0.43 ± 0.07% w/w, extract specific grafity 0.81 ± 0.01. Lagerstroemia loudonii  fruit extract, total ash content 11.45 ± 1.16%w/w, water-soluble ash content 10.1 ± 1.49% w/w, acid insoluble ash content 1.46 ± 0.88% w/w,extract specific grafity 0.81 ± 0.01. Based on phytochemical screening data and the characterization of bungur plants potential to be developed into raw materials for traditional medicineKeywords: Lagerstroemia loudonii, secondary metabolite, raw material characterization

scholarly journals Analytical Characterization of an Inulin-Type Fructooligosaccharide from Root-Tubers of Asphodelusramosus L

2021 ◽  
Vol 14 (3) ◽  
pp. 278
Author(s):  
Valentina Noemi Madia ◽  
Daniela De Vita ◽  
Antonella Messore ◽  
Chiara Toniolo ◽  
Valeria Tudino ◽  
...  
Keyword(s):  
Degree Of Polymerization ◽  
Water Soluble ◽  
Extensive Literature ◽  
Alkaline Extraction ◽  
Alkaline Extract ◽  
Therapeutic Uses ◽  
Ft Ir ◽  
Ir Analysis ◽  
Phytochemical Studies

Plant-based systems continue to play a pivotal role in healthcare, and their use has been extensively documented. Asphodelus L. is a genus comprising various herbaceous species, known by the trivial name Asphodelus. These plants have been known since antiquity for both food and therapeutic uses, especially for treating several diseases associated with inflammatory and infectious skin disorders. Phytochemical studies revealed the presence of different constituents, mainly anthraquinones, triterpenoids, phenolic acids, and flavonoids. Although extensive literature has been published on these constituents, a paucity of information has been reported regarding the carbohydrate composition, such as fructans and fructan-like derivatives. The extraction of water-soluble neutral polysaccharides is commonly performed using water extraction, at times assisted by microwaves and ultrasounds. Herein, we reported the investigation of the alkaline extraction of root-tubers of Asphodelus ramosus L., analyzing the water-soluble polysaccharides obtained by precipitation from the alkaline extract and its subsequent purification by chromatography. A polysaccharide was isolated by alkaline extraction; the HPTLC study to determine its composition showed fructose as the main monosaccharide. FT-IR analysis showed the presence of an inulin-type structure, and NMR analyses allowed us to conclude that A. ramosus roots contain polysaccharide with an inulin-type fructooligosaccharide with a degree of polymerization of 7–8.

scholarly journals Qualitative Phytochemical Analysis of Incarvillea emodi (Royle ex Lindl.) Chatterjee and Malva neglecta (Wallr.)

2020 ◽  
Vol 10 (4) ◽  
pp. 93-95
Author(s):  
M. Huzafa ◽  
Z. Jamal ◽  
A. Ahmed
Keyword(s):  
Bioactive Compounds ◽  
Plant Material ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Aerial Parts ◽  
Isolation And Characterization ◽  
Confirmatory Tests ◽  
Phytochemical Profile ◽  
Positive Results

The present study was carried out to investigate the phytochemical profile of Incarvillea emodi and Malva neglecta. The two plants were screened for the presence of nine different classes of bioactive compounds. The qualitative tests were performed on the aerial and underground parts of the selected plants to relate the presence of phytochemicals with bioactivities of the plants. The selected plant material was extracted with three different solvents. These extracts were then subjected to phytochemical screening and the presence of various phytochemicals was confirmed by different standard confirmatory tests. Most of the phytochemicals were found to be present. Results showed that the underground parts of Incarvillea emodi contain comparatively greater number of phytochemicals than the aerial parts. Whereas, in case of Malva neglecta the aerial parts showed more positive results than the underground parts. The current study offers proof for the presence of important bioactive compounds in the studied plants and recommends isolation and characterization of these compounds in the future.

scholarly journals MICROSCOPIC, PHYSICOCHEMICAL AND PHYTOCHEMICAL SCREENING OF CRATEVA MAGNA (LOUR) DC. (LEAF)

2017 ◽  
pp. 201-204
Author(s):  
Abirami J. ◽  
Jothi G. ◽  
Brindha P.
Keyword(s):  
Aqueous Extract ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Lower Epidermis ◽  
Foreign Matter ◽  
Preliminary Screening ◽  
Drug Discovery And Development ◽  
Microscopic Studies ◽  
Physicochemical Constants

Objective: Preliminary screening of phytochemicals is a valuable step, in the detection of the bioactive principles present in medicinal plants and subsequently may lead to drug discovery and development. In the present study, the identity, purity and strength of Crateva magna (Lour) DC. (Leaf), HPTLC, LC MS and phytochemical screening were analysed.Methods: Tests for Identity, Purity, and Strength of Crateva magna (Lour) DC. (Leaf) Botanical standards, Physicochemical constants such as Foreign matter, Total ash, moisture content, Acid insoluble ash, and water soluble ash and Extractive value such as Hexane, Chloroform, Ethyl acetate, Alcohol were determined. The Preliminary Phytochemical Analysis of various extracts was also determined. HPTLC, LCMS ANALYSIS was analysed.Results: Microscopic studies T S of Crateva magna leaf shows the presence of prominent midrib, upper and lower epidermis and lamina. Preliminary phytochemical analysis of dry powder and aqueous extracts of Crateva magna (Lour) DC. (Leaf) showed the presence of flavones, steroids, glycosides, sugar, alkaloids, quinones, phenols, coumarin and lignin. HPTLC of an Aqueous extract of the Crateva magna (Lour) DC. (Leaf). LC MS spectrum indicates may be the presence of bioactive compounds.Conclusion: The results revealed that aqueous extract of Crateva magna (Lour) DC. (Leaf) has suitable for anti ulcer study.

Standardization and Phytochemical screening of Carica papaya seeds

2021 ◽  
pp. 4540-4546
Author(s):  
Varsha Singh ◽  
Aleza Rizvi ◽  
Udaivir Singh Sara
Keyword(s):  
Physicochemical Parameters ◽  
Plant Secondary Metabolites ◽  
Chemical Properties ◽  
Fluorescence Analysis ◽  
Water Soluble ◽  
Correct Identification ◽  
Phytochemical Screening ◽  
Physico Chemical ◽  
Hexane Extracts ◽  
Phytochemical Studies

The present work focus to evaluate the physicochemical and preliminary phytochemical studies on the seeds of family Caricaceae. The plants resources which are used in pharmaceutical formulation standardization was carried out on the basis of organoleptic properties, physical characteristics, and physico-chemical properties. Different Physicochemical parameters ash values, extractive values, loss on drying, foreign matter, fluorescence analysis, and pH were evaluated. Macroscopical characteristics and Physicochemical parameters like total ash, acid insoluble ash and water soluble ash were found to be 83.7%, 71.7% and 61.5% w/w respectively. Hexane, ethyl acetate, ethanol and water soluble extractive values (hot)were 7.6%, 11.6%, 27.4%, 37.5%w/w respectively. The pH of 1% and 10% aqueous solution was found to be 3.57 and 3.78 respectively. Preliminary phytochemical screening showed the presence of Tannins, Proteins and amino acids, Glycosides, Phenolic compounds, Carbohydrates, Saponins, Alkaloids and Flavonoids. Thin layer chromatographic studies also had been done on ethanolic and hexane extracts. HPTLC fingerprinting is a valuable method for the quantitative determination of phytochemicals present in plant extract. These studies aim to investigating referential information for correct identification and standardization of this plant secondary metabolites.

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