scholarly journals Frequency and Distribution of Fok1rs 2228570 of Vitamin D Receptor Gene among Healthy Sudanese Population

2021 ◽  
Vol 11 (5) ◽  
pp. 61-64
Author(s):  
Amira S. Khalafalla ◽  
Yousif A. Mohammed ◽  
Adam D. Abakar ◽  
A Mergani ◽  
Amer M. Dafaalla ◽  
...  
Keyword(s):  
Vitamin D ◽  
Dna Extraction ◽  
Vitamin D Receptor ◽  
Receptor Gene ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Receptor Protein ◽  
Cross Sectional ◽  
Study Results ◽  
Area Survey

Background: Vitamin D plays a role in critical cellular processes such as apoptosis, cell development, and division. The Fok1 polymorphism in the VRD gene is T/C resulting in larger vitamin D receptor protein with lesser expression activity. Fok1 polymorphism is thought to be linked to many diseases including diabetes mellitus, cancers, and rheumatoid arthritis. The snipe also provides effective and sensitive diagnostic and predictive tools and affected by differences in ethnicity and individuals' life style. This study reports on the frequency and distribution of Fok1 SNP among healthy Sudanese transplantation donors. Method: This cross sectional study was conduct in National Cancer Institute University of Gezira, Sudan. One hundred and forty six healthy Sudanese transplantation donors were enrolled in this study. Two ml of venous blood was collected from each participant and stored at -20 till DNA extraction. Qaigen kit was used for DNA extraction. VDR Fok1 was genotyped by CTPP-PCR with proper primers designing and PCR condition. Data was collected by structured questionnaire and analyzed by SNP Stats online tool and medical calculator. Results: According to study results theFok1 polymorphism distributed among Sudanese with percentage of 49.9% (TT + CT), frequency of SNP variants was 69.8% regarding C allele and 30.2% for T allele, while the three genotypes was 10.2% for TT, 39.7% for TC and 50.1% for CC genotype. Conclusion: The frequency of Fok1 VDR SNP was high among healthy Sudanese transplantation donors, this necessitate the conduction of wide area survey among other healthy Sudanese population. This intern will help in investigating the nature of the relation of this SNP with other different diseases. Keywords: Vitamin D, Fok1rs. 2225870, VDR SNP, Sudanese

Interaction between a variant of vitamin D receptor gene and a posteriori dietary patterns on metabolic syndrome and its components

2018 ◽  
Vol 48 (5) ◽  
pp. 780-794 ◽  
Author(s):  
Fatemeh Shokrzadeh ◽  
Zahra Aslani ◽  
Abbas Rahimi-Foroushani ◽  
Sakineh Shab-Bidar
Keyword(s):  
Metabolic Syndrome ◽  
Vitamin D ◽  
Vitamin D Receptor ◽  
Dietary Patterns ◽  
Receptor Gene ◽  
Cross Sectional Study ◽  
Serum Glucose ◽  
Vdr Gene ◽  
Cross Sectional ◽  
Content Type

Purpose This paper aims to investigate whether the interaction between vitamin D receptor (VDR) FokI polymorphism and dietary patterns is associated with metabolic syndrome (MetS) and its components. Design/methodology/approach In total, 304 Iranians were enrolled in this cross-sectional study. Dietary patterns were identified using factor analysis. Fasting serum glucose and lipid profile were also assessed. FokI polymorphism of the VDR gene was genotyped using the restriction fragment length polymorphism method. Findings Individuals in third tertile of “Unhealthy Patterns had greater odds for MetS (odds ratio: 2.9; 95 per cent CI: 1.3, 6.1; P for trend = 0.03) compared to those in first tertile. Significant results disappeared after controlling for covariates (p = 0.09). There was no significant relationship between adherence to ‘Healthy Pattern’ and odds of MetS (p = 0.55). There were not any interactions between FokI polymorphism and major dietary patterns associated with MetS. Originality/value No evidence found for the interaction between polymorphism FokI and major dietary patterns associated with MetS and its components in Iranian subjects. Genome-wide association techniques are needed to assess the direct effect of this polymorphism on MetS.

scholarly journals Vitamin D status among adolescents in Europe: the Healthy Lifestyle in Europe by Nutrition in Adolescence study

2011 ◽  
Vol 107 (5) ◽  
pp. 755-764 ◽  
Author(s):  
Marcela González-Gross ◽  
Jara Valtueña ◽  
Christina Breidenassel ◽  
Luis A. Moreno ◽  
Marika Ferrari ◽  
...  
Keyword(s):  
Vitamin D ◽  
Healthy Lifestyle ◽  
Cross Sectional Study ◽  
Skeletal Structure ◽  
Specific Reference ◽  
Childhood And Adolescence ◽  
Vitamin D Status ◽  
Geographical Differences ◽  
Cross Sectional ◽  
Study Results

An adequate vitamin D status is essential during childhood and adolescence, for its important role in cell growth, skeletal structure and development. It also reduces the risk of conditions such as CVD, osteoporosis, diabetes mellitus, infections and autoimmune disease. As comparable data on the European level are lacking, assessment of vitamin D concentrations was included in the Healthy Lifestyle in Europe by Nutrition in Adolescence (HELENA) study. Fasting blood samples were obtained from a subsample of 1006 adolescents (470 males; 46·8 %) with an age range of 12·5–17·5 years, selected in the ten HELENA cities in the nine European countries participating in this cross-sectional study, and analysed for 25-hydroxycholecalciferol (25(OH)D) by ELISA using EDTA plasma. As specific reference values for adolescents are missing, percentile distribution were computed by age and sex. Median 25(OH)D levels for the whole population were 57·1 nmol/l (5th percentile 24·3 nmol/l, 95th percentile 99·05 nmol/l). Vitamin D status was classified into four groups according to international guidelines (sufficiency/optimal levels ≥ 75 nmol/l; insufficiency 50–75 nmol/l; deficiency 27·5–49·99 nmol/l and severe deficiency < 27·5 nmol/l). About 80 % of the sample had suboptimal levels (39 % had insufficient, 27 % deficient and 15 % severely deficient levels). Vitamin D concentrations increased with age (P < 0·01) and tended to decrease according to BMI. Geographical differences were also identified. Our study results indicate that vitamin D deficiency is a highly prevalent condition in European adolescents and should be a matter of concern for public health authorities.

scholarly journals Geographic and Seasonal Variation of Vitamin D: A Retrospective Study in Two Centers of Nepal

2020 ◽  
Vol 18 (1) ◽  
pp. 103-107
Author(s):  
Arun Sedhain ◽  
Gandhi R Bhattarai ◽  
Sanjaya Raya Yadav ◽  
Bhupendra Raj Pandey ◽  
Thakur Prasad Pant
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Winter Season ◽  
Hypovitaminosis D ◽  
Cross Sectional Study ◽  
Reproductive Age ◽  
Age Group ◽  
Cross Sectional ◽  
Study Results ◽  
The Status

Background: Vitamin D deficiency is a common problem in many countries throughout the world. This study aimed at understanding the status of vitamin D deficiency in relation to age, gender, geography and season among relatively bigger numbers of population residing in central and western regions of Nepal.Methods: This retrospective cross-sectional study was carried out by reviewing the records of vitamin D tests in two centers of Nepal- namely united reference laboratories at Pokhara and Chitwan Medical College at Chitwan. A total of 3320 individuals who were tested for clinical suspicion of vitamin D deficiency over a period of one year were included in the study. Results: Mean vitamin D level was 18.7 ng/mL. Majority of subjects tested for vitamin D were females (71.36 %) with female to male ratio of 2.49:1. Hypovitaminosis D (? 30 ng/mL) was found in 84.5% subjects and was moderate to severely deficit (<10 ng/mL) in 25.9% of subjects. Status of hypovitaminosis D was found to be less in ?50 years (79.9%) than the younger subjects (89.9% in ?18 years and 87.0% in 19-49 years age group). Vitamin D deficiency was found lower in hills districts (80.8% vs 89.7% in the plains), which was found to be more pronounced during winter through summer seasons as compared to fall season.Conclusions: Population residing in plain areas and summer, spring and winter season are found to have increased problem of Hypovitaminosis D. It is also found to be common among younger children and female of reproductive age group. We have also found out the increased problem of hypovitaminosis D among population residing in the plain areas than in the hills especially in the summer, spring and winter season.Keywords: Geographic variation; hypovitaminosis D; Nepal

scholarly journals Vitamin D receptor gene expression in mammalian kidney.

1994 ◽  
Vol 5 (5) ◽  
pp. 1251-1258
Author(s):  
L Liu ◽  
M Ng ◽  
A M Iacopino ◽  
S T Dunn ◽  
M R Hughes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Vitamin D Receptor ◽  
Collecting Duct ◽  
Receptor Gene ◽  
Receptor Protein ◽  
Vitamin D Receptor Gene ◽  
Cortical Collecting Duct ◽  
Distal Nephron ◽  
Receptor Gene Expression

The vitamin D-receptor protein and its mRNA were localized in microscope sections of paraffin-embedded mammalian kidneys by means of immunocytochemistry and in situ hybridization, respectively. A monoclonal antibody against chicken intestinal vitamin D receptor immunostained the nucleus and cytoplasm of cells within the distal convoluted tubule, connecting segment, and initial cortical collecting duct of both rats and pigs. Although fainter, immunostaining also was present over proximal tubular cells. (35S)UTP-labeled cRNA probes were detected over both the proximal and distal portions of the mouse nephron, but silver grain densities were 5.8-fold greater over the latter. In conclusion, localization of both the vitamin D-receptor protein and its mRNA in both the proximal and distal nephron of adult mammals suggests that the gene for this protein is expressed in cells at both of these sites. The intensity of immunostaining and the density of cRNA-associated silver grains suggest that vitamin D-receptor gene expression is greatest in the distal nephron.

scholarly journals Vitamin D epimers are associated with circulating haemoglobin levels independently of C-reactive protein

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
La-or Chailurkit ◽  
Piyamitr Sritara ◽  
Prin Vathesatogkit ◽  
Sukit Yamwong ◽  
Nisakron Thongmung ◽  
...  
Keyword(s):  
Vitamin D ◽  
Venous Blood ◽  
High Sensitivity ◽  
Cross Sectional Study ◽  
Total Serum ◽  
C Reactive Protein ◽  
Cross Sectional ◽  
Reactive Protein ◽  
Long Term Follow Up

AbstractVitamin D deficiency has been shown to be associated with anaemia. Circulating 25(OH)D consists of both epimeric and nonepimeric forms. However, the relative roles of epimeric and nonepimeric vitamin D in regulating anaemia and haemoglobin levels remain unknown. Therefore, in this study, we examined the effect of vitamin D, including its epimers, on haemoglobin levels, independently of its effect on circulating high-sensitivity C-reactive protein (hsCRP). This was a cross-sectional study of 1655 subjects from a long-term follow-up cohort at the Electricity Generating Authority of Thailand. Venous blood sample were collected for determination of vitamin D [25(OH)D2, 25(OH)D3, 3′-epi-25(OH)D2, and 3′-epi-25(OH)D3], haemoglobin, and hsCRP levels. Data are presented as mean ± standard deviation. Age, sex, and body mass index (BMI) were significantly associated with circulating haemoglobin levels, while no association was found between total serum 25(OH)D and haemoglobin levels. However, when total 25(OH)D was separated into 3′-epimeric and non-3′-epimeric forms, 3′-epi-25(OH)D was significantly associated with haemoglobin levels, independently of age, sex, and BMI (P < 0.01). No association was found between non-3′-epi-25(OH)D and haemoglobin. When hsCRP was added to the model, the effect 3′-epi-25(OH)D on haemoglobin levels remained significant (P < 0.01). In conclusion, vitamin D epimers are associated with circulating haemoglobin levels, which supports the role of vitamin D in red blood cell and iron physiology.

scholarly journals Vitamin D Status of Adults in the Outpatient Department in Bangladesh

2018 ◽  
Vol 27 (1) ◽  
pp. 94-97
Author(s):  
NA Chowdhury ◽  
Mohammad Zaid Hossain ◽  
MM Mia ◽  
S Hoque ◽  
NA Chowdhury ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Adult Population ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Vitamin D Insufficiency ◽  
Outpatient Department ◽  
Cross Sectional ◽  
Hydroxyvitamin D ◽  
Males And Females

Background: Vitamin D deficiency (VDD) is widespread, yet it is the most underdiagnosed and undertreated nutritional deficiency in the world. The prevalence of VDD is estimated to affect over 1 billion people worldwide. The present study was conducted to estimate the prevalence of VDD among adults aged 18–89 years visiting the outpatient department in a clinic/chamber. Objective: To assess the status of vitamin D deficiency (VDD) among the adult population in Bangladesh. Methods: A cross sectional study was conducted among 189 adults in the year 2017 including both males and females aged 18–89 years in outpatient department of a clinic/chamber. Venous blood sample was collected for the measurement of Vitamin D (25 hydroxyvitamin D). Results: This study included a total of 189 adults (29 males and 160 females) aged between 18 and 89 years. Majority of the patients were vitamin D deficient, 67.2% and 20.6% were suffering from vitamin D insufficiency. Maximum males and females are deficient, 69% and 66.9% respectively where as a very small percentage has sufficient level of vitamin D. Conclusions: Vitamin D deficiency (VDD) is alarmingly high. Screening services should be implemented while researchers focus on strategies to lessen the incidence and morbidity associated with these conditions. J Dhaka Medical College, Vol. 27, No.1, April, 2018, Page 94-97

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