scholarly journals Evaluation of preliminary phytochemical screening, acute toxicity and antioxidant profile of Ocimum kilimandscharicum

2019 ◽  
Vol 9 (2) ◽  
pp. 372-375 ◽  
Author(s):  
Gaurav Saxena ◽  
Abhilasha Mittal ◽  
Abdul Wadood Siddiqui
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Sprague Dawley ◽  
Oral Toxicity ◽  
Clinical Safety ◽  
Free Radical Activity ◽  
In Vitro Antioxidant Activity

Nature has offered us diverse curative herbs having with powerful antioxidant phytochemicals. Ocimum (Lamiaceae) is a notable source of volatile oils and flavouring agents in general and primarily of eugenol, methyl eugenol, linalool, methyl chavicol, etc. Karpoora Thulasi is a member of this genus; nevertheless, not much literature has been reported on its safety and antioxidant potential. In this investigation, we did a pre-clinical safety assessment of concentrate of O. kilimandscharicum on Sprague Dawley rodents. Toxicological concordat of the O. kilimandscharicum concentrate was carried out following OECD guidelines 423. Further, to verify the traditional efficacy and elucidate the mechanism, the present study compared in-vitro antioxidant activity of the plant by DPPH, ABTS and Hydroxyl radical scavenging method using ascorbic acid as the standard. In acute oral toxicity, no treatment-related death or toxic signs were observed. Moreover, the study revealed that the O. kilimandscharicum extract could be well tolerated up to the dose 2000 mg/kg body weight and could be classified as Category 5. Moreover, ABTS free radical activity of the extract was 79.2148 while that of DDPH Inhibition potential was found to be 70.72758. Our findings present substantiation that the crude extracts of O. kilimandscharicum is a likely source of natural antioxidants, and this justified its long-established uses.  Keywords: Ocimum kilimandscharicum, Acute Toxicity, In-vitro Antioxidant Activity DPPH, ABTS

scholarly journals Antioxidant Activity of Endophytic Fungi Isolated from Turmeric Flowers

2020 ◽  
Vol 12 (2) ◽  
pp. 268-273
Author(s):  
Eris Septiana ◽  
Yadi Yadi ◽  
Partomuan Simanjuntak
Keyword(s):  
Antioxidant Activity ◽  
Endophytic Fungi ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
The Body ◽  
Mixed Cultures ◽  
Additional Information ◽  
Single And Mixed Cultures ◽  
In Vitro Antioxidant Activity

Antioxidants are compounds needed by the body to fight free radicals that can cause adverse effects on health. All parts of turmeric plant have antioxidant activity, so does the flower. It is thought that the endophytic fungi from turmeric flowers also have antioxidant activity and no studies have reported it yet. The purpose of this study was to study the antioxidant activity of single and mixed cultures of endophytic fungi isolated from turmeric flowers. In vitro antioxidant activity assay was conducted using the DDPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging method by measuring its absorption using a UV-Vis spectrophotometer at a wavelength of 517 nm. The extract of single and mixed cultures of endophytic fungi isolated from turmeric flowers showed antioxidant activity. The combination of fungal cultures can increase the antioxidant activity of a single culture. This study is the first to report that the combination of endophytic fungi from turmeric flowers can increase its antioxidant activity. Therefore, the presence of the endophytic fungi in turmeric flowers that have antioxidant activity can provide additional information so that the utilization of turmeric flowers as a source of natural antioxidants will increase.

scholarly journals QUANTITATIVE ANALYSIS OF NATURAL ANTIOXIDANTS AND FREE RADICAL SCAVENGING ACTIVITIES OF CRUCIFERAE VEGETABLES

2017 ◽  
Vol 10 (4) ◽  
pp. 470
Author(s):  
Jamuna K S ◽  
Ramesh C K ◽  
Aditya Rao Sj ◽  
Paramesha M ◽  
Riaz Mahmood
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Plant Materials ◽  
Cruciferous Vegetable ◽  
The Relationship ◽  
In Vitro Antioxidant Activity

Objective: The aim of this research program is to evaluate the antioxidant potentialities comprehensively utilizing commonly available cruciferous vegetables of India, viz., cabbage, cauliflower, kohlrabi, and radish.Methods: The plant materials were procured and processed for cold extraction procedure using 70% ethanol. The extracts were primarily assessedfor their phytoconstituents and further for their in vitro antioxidant activity using various qualitative and quantitative estimations. The results of quantitative estimations were expressed in terms of equivalence with respective standards, and the antioxidant potentiality of various scavenging and chelating activities were expressed in terms of 50% effective concentration. Further, correlation studies were made between quantitative and qualitative assays to study the relationship between the effects of different phytoconstituents groups.Results: In this study, the results revealed that all the four cruciferous vegetable extracts possess potential antioxidant activities. Among the extractsunder study kohlrabi has recorded superior antioxidant potential than others. It is clear from the study that the tested cruciferous vegetablesmanifested differential expression of antioxidant capacity due to their phytoconstituents.Conclusion: From the results, it can be concluded that the Cruciferae vegetables have potent antioxidant activity contributing to the use for healthbenefits in addition to their nutritive role as vegetable.Keywords: Cruciferae vegetables, Phytochemicals, In vitro antioxidant activity, Correlation.

scholarly journals Antioxidant and Hypoglycemic Properties of Seeds of Cucurbitaceae Family from Western Nepal

2019 ◽  
Vol 9 (2) ◽  
pp. 1-5
Author(s):  
Atisammodavardhana Kaundinnyayana ◽  
Anita G.C ◽  
Asmita Banstola ◽  
Kishwor Poudel ◽  
Nirmal Acharya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Lagenaria Siceraria ◽  
Benincasa Hispida ◽  
Ic50 Values ◽  
In Vitro Antioxidant Activity

Introduction: Diabetes mellitus is an endocrine and metabolic disorder characterized by the defect in insulin secretion or insulin action or both. The association between diabetes mellitus and oxidative stress is well established. Cucurbitaceae is the largest family containing 120 genera mostly grown for its sweet and juicy fruit in warm climates all over the world. In the present work ethanolic extracts of the seeds of Trichosanthes cucumerina Linn (Chichinno), Lagenaria siceraria (Lauka), Cucurbita pepo (Pharsi), Luffa aegiptiaca (Ghiraula), and Benincasa hispida (Kubindo) collected from Western Nepal has been evaluated for their in vitro antioxidant activity and in vivo hypoglycemic effects. Methods: In vitro antioxidant activity was assessed by using DPPH free radical scavenging activities and their IC50 values were calculated. In vivo hypoglycemic effect was examined on normoglycemic rats. The clinical significance of ethanolic extract at the doses of 250 and 500 mg/kg body weight was investigated in 0, 30, 60, 120 and 180 minutes of oral administration. Metformin treated group was used as the positive control. Results: For DPPH radical scavenging action, the IC50 values of Trichosanthes cucumerina, Luffa aegiptiaca, Benincasa  hispida, Cucurbita pepo, Lagenaria siceraria were found to be 60.72, 127.73, 49.63, 98.16 and 52.46 µg/ml respectively which were compared to the IC50 value of ascorbic acid (Positive control) which was found to be 38.11 µg/ml. The extracts of Benincasa hispida, and were having higher antioxidant activity and tested for in vivo hypoglycemic activity.In vivo administration of two doses of ethanolic extract of Lagenaria siceraria reduced the level of blood glucose while the best result was obtained at 250 mg/kg. Conclusions: Present study revealed promising antioxidant and hypoglycemic activity of ethanolic extract of Lagenaria siceraria. The further exploration of Lagenaria siceraria for its effective use in the traditional medicinal system is essential.

scholarly journals Purification, characterization and antioxidant activities in vitro of polysaccharides from Amaranthus hybridus L.

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9077
Author(s):  
Zizhong Tang ◽  
Caixia Zhou ◽  
Yi Cai ◽  
Yujia Tang ◽  
Wenjun Sun ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Structural Characteristics ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Monosaccharide Composition ◽  
In Vitro Test ◽  
Amaranthus Hybridus ◽  
Total Antioxidant

Background Amaranthus hybridus L. is an annual, erect or less commonly ascending herb that is a member of the Amaranthaceae family. Polysaccharides extracted from traditional Chinese medicines may be effective substances with antioxidant activity. Methods In this study, we isolated crude polysaccharides from A. hybridus (AHP-M) using microwave-assisted extraction. Then, the AHP-M was purified by chromatography with DEAE-32 cellulose, and two fractions, AHP-M-1 and AHP-M-2, were obtained. The structural characteristics of AHP-M-1 and AHP-M-2 were investigated, and their antioxidant activities were analyzed in vitro. Results We found that the monosaccharide composition of AHP-M-1 was different from that of AHP-M-2. The molecular weights of AHP-M-1 and AHP-M-2 were 77.625 kDa and 93.325 kDa, respectively. The results showed that the antioxidant activity of AHP-M-2 was better than that of AHP-M-1. For AHP-M-2, the DPPH radical scavenging rate at a concentration of 2 mg/mL was 78.87%, the hydroxyl radical scavenging rate was 39.34%, the superoxide anion radical scavenging rate was 80.2%, and the reduction ability of Fe3+ was approximately 0.90. The total antioxidant capacity per milligram of AHP-M-2 was 6.42, which was higher than that of Vitamin C (Vc). Conclusion The in vitro test indicated that AHP-M-1 and AHP-M-2 have good antioxidant activity, demonstrating that A. hybridus L. polysaccharide has immense potential as a natural antioxidants.

scholarly journals Chromatographic and Spectroscopic Fingerprinting of Ficus carica and Evaluation of In Vitro Antioxidant Activity

2021 ◽  
Vol 25 (03) ◽  
pp. 677-682
Author(s):  
Amara Javaid
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Ficus Carica ◽  
In Vitro Antioxidant Activity

The present study was conducted to evaluate the in vitro antioxidant activity of Ficus carica, commonly known as fig. Methanol and ethanol extracts of F. carica leaves were subjected to 2, 2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity assay where ascorbic acid being positive control had an IC50 value of 3.98±0.26 while methanol and ethanol fractions showed an IC50 of 101.76±1.12 and 93.12±1.17 respectively exhibiting their high antioxidant potential. DPPH assay was also performed on high performance liquid chromatography (HPLC) elutions. Most active antioxidant components in ethanol extract were eluted between 17–18 min, and those in methanol were eluted over 14–15 min and upon ultra-high performance liquid chromatography-mass spectrometery (Orbitrap Liquid Chromatography-Mass Spectrometry) were identified to be 13-Docosenamide, (Z)- for ethanol and ficusin for methanol fraction. Thus, it is concluded that these two components are most probable determinants of antioxidant potential of F. carica leaf extracts. © 2021 Friends Science Publishers

scholarly journals Antioxidant Activity of Plant Extracts from Colombian Coffee-Growing Eco-Region

2017 ◽  
Vol 13 (1) ◽  
pp. 56-59
Author(s):  
Aura M. Blandón ◽  
Oscar M. Mosquera ◽  
Antônio E. G. Sant’ana ◽  
Aldenir F. Dos Santos ◽  
Luana L. S. Pires
Keyword(s):  
Antioxidant Activity ◽  
Plant Species ◽  
Plant Extracts ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Dpph Assay ◽  
Methanolic Extracts ◽  
In Vitro Antioxidant Activity

The present study describes the in vitro antioxidant activity of methanol extracts of 34 plant species collected in the Colombian coffee-growing eco-region belonging to Euphorbiaceae, Piperaceae and Solanaceae families. The antioxidant properties of extracts were evaluated by determining radical scavenging power measured with a DPPH assay. The methanolic extracts of  Hyeronimia antioquiensis, Mabea montana, and Alchornea grandis species (Euphorbiaceae), presents EC50 values equal to 0.686, 12.35, and 13.01 µg/mL, respectively, showing high antioxidant potential.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND WOUND HEALING ACTIVITY OF WHEATGRASS BY 1,1- DIPHENYL, 2 PICRYLHYDRAZYL METHOD

2018 ◽  
Vol 11 (12) ◽  
pp. 170
Author(s):  
Veermaneni Alekhya ◽  
Thiyagarajan Deepan ◽  
Magharla Dasaratha Dhanaraju
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Wheat Plant ◽  
Wide Range ◽  
Wound Healing Activity ◽  
In Vitro Antioxidant Activity

Objective: This study was designed to evaluate in vitro antioxidant activity and wound healing activity in Triticum aestivum (wheat grass).Methods: T. aestivum commonly known as Wheatgrass had a wide range of health benefits among the young grass of common wheat plant components includes chlorophyll, flavonoids, and Vitamins A, C, and E. Wheatgrass is used in Folklore medicine for treatment of skin diseases and wound healing. In our present study, petroleum ether, ethanol and aqueous extracts of T. aestivum have been evaluated for in vitro antioxidant activity and wound healing activity by 1,1- diphenyl, 2 Picrylhydrazyl radical scavenging activity, and Chick chorioallantoic method, respectively.Results: The results of both the assay showed that all the extracts of T. aestivum have significant antioxidant and wound healing activity on dose-dependent manner.Conclusion: The wheatgrass has antioxidant and wound healing activity.

In Vitro Antioxidant Activity of Pigment Extracted from Fruits of Padus racemosa and Padus virginiana

2011 ◽  
Vol 183-185 ◽  
pp. 565-569 ◽  
Author(s):  
Jian Ren ◽  
Zhen Yu Wang
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Reducing Power Assay

The total anthocyanin content (TAC) of pigment extracted from fruits of Padus racemosa and Padus virginiana was determined by pH-differential method. TAC in pigment from fruits of Padus racemosa was higher than that in Padus virginiana. The vitro antioxidant activity of the two kinds of pigment was evaluated by different assays, including DPPH• assay, ABTS•+assay, OH• assay and reducing power assay. The results showed that except for reducing power, pigment from fruit of Padus racemosa showed stronger ABTS•+, DPPH• and OH• radical scavenging activity than pigment from fruit of Padus virginiana. The study concluded that pigment extracted from fruit of Padus racemosa and Padus virginiana can be used as a source of natural antioxidants instead of synthetic antioxidants.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY, IN VIVO ANTIPYRETIC AND ANTI-INFLAMMATORY ACTIVITIES OF PARONYCHIA CAPITATA L.

2017 ◽  
Vol 10 (3) ◽  
pp. 177
Author(s):  
Soumia Bouzidi ◽  
Benkiki Naima ◽  
Allaoua Zina
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Butanol Extract ◽  
Antipyretic Activity ◽  
Male Wistar Rats ◽  
Anti Inflammatory ◽  
In Vitro Antioxidant Activity

ABSTRACTObjective: The aim of this study is to evaluate the in vitro antioxidant activity of Paronychia capitata various extracts, acute toxicity, the in vivoantipyretic and anti-inflammatory activities of the n-butanol extract.Methods: The antioxidant properties were tested using 2,2-diphenyl 1-picrylhydrazyl radical scavenging and β-carotene linoleic acid system, thetotal phenolic and flavonoid contents were determined. Furthermore, anti-inflammatory activity and antipyretic activity of the n-butanol extractwere assessed on male Wistar rats at the dose levels 250, 500 mg/kg body weight, using the egg albumin-induced edema, and brewer’s yeast-inducedpyrexia method, respectively.Results: The n-butanol extract of P. capitata had considerably the highest antiradical activity with inhibitory concentration 50 value of 8.667 μg/ml.Conclusion: The experimental data demonstrated that n-butanol extract of P. capitata possess significantly remarkable anti-inflammatory andantipyretic activities compared to the standard drugs.Keywords: Caryophyllaceae, Flavonoid, Inflammation, Fever, Paronychia capitata.

scholarly journals Biopolymer from Adenanthera pavonina L. Seeds: Characterization, Photostability, Antioxidant Activity, and Biotoxicity Evaluation

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Roberta Cardoso Melo ◽  
Maurycyo Silva Geronço ◽  
Rayran Walter Ramos Sousa ◽  
Lara Polyana Silva Ramos ◽  
Francisca Pereira Araújo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Acute Toxicity ◽  
Uv Irradiation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Adenanthera Pavonina ◽  
Pharmaceutical Industries

Plant polysaccharides have been increasingly employed in the pharmaceutical, industrial, and food environments due to their versatile functional properties. In the present investigation, a heteropolysaccharide galactomannan (GAP) was extracted from Adenanthera pavonina L. seeds and characterized by physicochemical analyses to determine its thermal properties, photostability, antioxidant activity, and acute toxicity. GAP was characterized by FTIR, DSC, and TG. The photostability of GAP submitted to artificial UV irradiation was analyzed. Antioxidant activity was evaluated by the DPPH (2,2-diphenyl-1-1-picrylhydrazyl) free radical-scavenging method, while a bioassay method was carried out to study acute toxicity in Artemia salina L. Physical-chemical and functional characteristics of GAP support its potential role in the food and pharmaceutical industries. GAP was photostable under UV irradiation. In vitro GAP antioxidant evaluation showed that it bears free radical-scavenging activity for DPPH radicals. The median lethal concentration (LC50) of GAP was 239.4 mg∙mL−1, indicating that this biopolymer is nontoxic. Such results indicate that this biopolymer presents characteristics of neutrality, photostability, and nontoxicity that are commercially attractive.

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