scholarly journals Deteksi Endoparasit Cacing pada Hepar Tikus Laboratorium (Rattus norvegicus) dari Sentra Peternak di Kabupaten Banyumas dan Kabupaten Purbalingga

Author(s):  
Dita Pratiwi Kusuma Wardani ◽  
Kurnia Ritma Dhanti ◽  
Arif Mulyanto ◽  
Tantri Analisawati Sudarsono

The presence of laboratory rats that are maintained and bred for laboratory purposes or laboratory observations is very necessary. The presence of endoparasite in laboratory rats will have an impact on the result of the research or laboratory observations. This study aims to detect helminth endoparasites in the liver of laboratory rats (Rattus norvegicus) from animal breeders in Banyumas and Purbalingga Districts. This research was an observational study with a cross-sectional design. A total of 52 laboratory rats were used in the study. Rats are killed with chloroform, liver surgery then identify the presence of worm larvae. Out of the 52 rats obtained, 7 (29.17%) from 24 laboratory rats in Banyumas District and 5 (17.86%) from 28 laboratory rats in Purbalingga District were infected with Taenia taeniaeformis. It is necessary to control helminth infections in laboratory rats, such as laboratory animal quarantine, health monitoring, and antihelmintic treatment. It is important to handle carefully during travel to assure the results of research or laboratory observations using the animals.

IKESMA ◽  
2017 ◽  
Vol 13 (2) ◽  
Author(s):  
Gading Giovani PutriPutri

To realize food safety is applying good personal hygiene and sanitation.This study aims to describe personal hygiene and foodstalls sanitation of pecel tumpang in Kediri.An observational study with cross sectional design done on 20 sampels. Data performed in table with descriptive analyzed. Most of respondents had quite personal hygiene (80%), good location and building (50%), poor waste disposal (50%), poor sanitation facilities(50%), good sanitation of equipments (100%), and good clean water (90%).Somevariables indicate poor sanitation i.e. waste disposal and sanitation facilities. Keywords: personal hygiene, food stalls sanitation, pecel tumpang


2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Berliana Irianti ◽  
Ermawati Ermawati ◽  
Arni Amir

Abstrak Penyebab dismenore belum semuanya diketahui, ada dugaan peningkatan proses peroksida lipid yang akan mengaktivasi mediator inflamasi pada endometrium yang menimbulkan rasa nyeri haid (dismenore). Tujuan penelitian ini adalah menentukan perbedaan kadar malondialdehide dan tromboksan B 2  pada dismenore dan tanpa dismenore. Studi observasional ini menggunakan desain potong lintang komparatif. Subjek penelitian terdiri dari dua kelompok yaitu 23 remaja dismenore dan 23 remaja tanpa dismenore dengan waktu penelitian dari Juni sampai Juli 2014. Analisis sampel dilakukan di Laboratorium Biomedik dan Biokimia Universitas Andalas Padang. Pemeriksaan kadartromboksan B 2  menggunakan metode ELISA dan kadar malondialdehide menggunakan metode Asam Thiobarbiturat (TBA). Hasil penelitian diperoleh bahwa rerata kadar malondialdehid pada remaja dengan dismenore yaitu 2,60±0,63 µmol/ml, rerata remaja tanpa dismenore 1,98±0,12 µmol/ml dengan probabilitas p<0,05 (0,000), sedangkan reratakadar Tromboksan B 2  pada remaja dengan dismenore 20,043±9,56 ng/ml, rerata remaja tanpa dismenore 19,222±10,79 ng/ml, dengan probabilitas p>0,05 (0,786). Kesimpulan penelitian ini adalah terdapat perbedaan yang signifikan rerata kadar malondialdehid pada remaja dengan dismenore dan tanpa dismenore dan tidak terdapatperbedaan signifikan pada kadar tromboksan B 2 pada remaja dengan dismenore dan tanpa dismenore.Kata kunci: remaja, dismenore, malondialdehide, tromboksan B2 Abstract The precise cause of dysmenorrhea is still unclear, there may be increased lipid peroxidation process will activate the inflammatory mediators at endometrium that cause menstrual cramps (dysmenorrhea). The objective of this study was to determine the difference of malondialdehyde levels and thromboxane B 2  levels in dysmenorrhea and without dysmenorrhea. It was an observational study with comparative cross-sectional design. The subjects consisted of two groups, they are 23 adolescent with dysmenorrhea and 23 adolescents without dysmenorrhea, done in Juny -July 2014. Sample analysis was conducted in Laboratory of Biochemistry and Biomedical Laboratory of Andalas University Padang. The examination of Thromboxane B 2  levels used ELISA and the examination of malondialdehyde levels used a Thiobarbituric acid method. The results showed the mean of malondialdehyde levels in adolescents withdysmenorrhea was 2.60±0.63 µmol/ml, the mean level in adolescent without dysmenorrhea was 1.98±0.12 µmol/ml with probability p<0.05 (0.000), while the mean levels of thromboxane B 2  in adolescents with dysmenorrhea was 20.043±9.56 ng/ml, the mean level in adolescent without dysmenorrhea was 19.222±10.79 ng/ml, with probabilityp>0.05 (0.786). It can be concluded that there is a significant difference in the mean of malondialdehyde levels between adolescents with dysmenorrhea and without dysmenorrhea and there is no significant differences in thromboxane B 2 level between adolescents with dysmenorrhea and without dysmenorrheaKeywords: adolescent, dysmenorrhea, malondialdehyde, thromboxane B2


Sains Medika ◽  
2015 ◽  
Vol 6 (1) ◽  
pp. 8
Author(s):  
Desia Laila Dian Saputri ◽  
Ika Rosdiana ◽  
Endang Lestari

Introduction: Stroke ranks as the first cause of disabilities and the third cause of death in the world. Many factors associated with stroke. Objective: this study aimed at evaluating the factors associated with stroke. Study Design: This study was an analytical observational study using cross sectional design. Samples were obtained from medical records of 263 patients who were admitted in neurology’s ward of Sultan agung Islamic hospital during 1 January 2012-31 August 2013. Samples categorized in 2 groups: 97 hemorrhagic’s stroke patients and 166 non hemorrhagic’s stroke patients with simple random sampling technique. This study were conducted by analyzing 5 variables such as gender, age, hypertension, diabetes mellitus and total cholesterol levels. Results: Logistic regression test using SPSS showed that hypertension was the most dominant factor associated with stroke (p=0.001; PR=1.707; CI=1.249–2.433). Conclusion: Hypertension was the most dominant risk factor for stroke.


e-CliniC ◽  
2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Gabriella A. Koagouw ◽  
Steward K. Mengko ◽  
Armenius R. Sondakh

Abstract: The most throat health problem is inflammation. Around 80% of sore throat are caused by viruses and only 10-20% are caused by bacteria. The types of inflammation found on the throat are tonsillitis and pharyngitis. This study aimed to obtain data of throat health among students of SMA Negeri 9 (Senior High School) Manado. This was a descriptive observational study with a cross sectional design. Respondents were 10th grade students of SMA Negeri 9 Manado. The results showed that most respondents had normal tonsil and pharynx. Conclusion: Most students of SMA Negeri 9 Manado had good throat health status. Keywords: throat health status, tonsil examination, pharynx examination Abstrak: Masalah kesehatan pada tenggorok yang terbanyak ditemukan ialah peradangan. Sekitar 80% radang tenggorok disebabkan oleh virus dan hanya 10-20% disebabkan oleh bakteri. Peradangan yang sering ditemukan pada tenggorok ialah tonsilitis dan faringitis. Penelitian ini bertujuan untuk mendapatkan gambaran kesehatan tenggorok pada siswa-siswi Sekolah Menengah Atas (SMA) Negeri 9 Manado. Penelitian ini menggunakan metode deskriptif observasional dengan desain potong lintang. Responden ialah siswa-siswi kelas X.4 Sekolah Menengah Atas (SMA) Negeri 9 Manado. Hasil penelitian menunjukkan bahwa umumnya responden mempunyai tonsil dan faring yang normal. Simpulan: Umumnya gambaran kesehatan tenggorok pada siswa-siswi Sekolah Menengah Atas (SMA) Negeri 9 Manado baik.Kata kunci: kesehatan tenggorok, pemeriksaan tonsil, pemeriksaan faring


2009 ◽  
Vol 8 (1) ◽  
pp. 11
Author(s):  
Barunawaty Yunus

Radiography as diagnostic tool is essential for treatment plan of implant. Manytechniques used to produce radiography are recommended to support the dentist inaccessing area of implantation. Many implant experts use panoramic radiography asguidelines of jaw bone evaluation as recommended by implant procedures. In orderto get optimal measurement, occlusal radiography technique can be added to evaluatethe bone thickness.panoramic and periapical radiography can solely evaluate thewidth and height of jaw bone. This study proposed to evaluate parametric value, aswidth, height, and thickness of jaw bone by conventional dental diagnosticradiography. This was observational study, with cross sectional design. Samples werechosen as population target that fulfilled samples criteria. Number of samples were 30and and analyzed using the SPSS program for Windows 14.0 and T-test. In this study,it can be concluded that there was significant difference before and after conversion ofall parametric radiography conventional tooth on jaw bone.


2013 ◽  
Vol 4 (3) ◽  
Author(s):  
Damajanty H.C. Pangemanan ◽  
Joice N.A. Engka ◽  
Ivonny M. Sapulete

Abstract. This study aimed to disclose the influence of noise on hearing and the correlation of noise on blood pressure in people who worked in game centers in Manado. This was an observational study using a cross sectional design. The data were obtained by measuring the noise level in three game centers in Manado, measuring the respondent’s hearing and blood pressure, and using questionnaires. Data were taken from three game centers in Manado. Data were obtained through questionnaires and the measurement of a minimal hearing level of noise, and blood pressure.  The number of samples were 25 people who worked in the game centers. Data were analyzed by using the Anova test with a significance level of p<0, for finding out the correlation between noise and blood pressure.The result showed that there were hearing disturbances in 60% of respondents who worked 7-8 hours/day, and in 100% of respondents who worked >8 hours/day. There was no significant correlation value beetween noise and blood pressure. Conclusion: Noise could cause hearing disturbances; however there was no correlation between noise and blood pressure among game center workers in Manado. Keywords: noise, blood pressure, deafness.     Abstrak. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh adanya kebisingan pada pendengaran dan tekanan darah pada para pekerja game center di kota Manado. Penelitian ini merupakan suatu studi observasional potong lintang. Data didapatkan dengan cara mengukur tingkat kebisingan pada tiga game center di kota Manado, pengukuran nilai ambang pendengaran dan tekanan darah, serta dengan menggunakan kuesioner.Data diambil dari 3 game center di Kota Manado. Data didapatkan melalui kuesioner dan pengukuran tingkat kebisingan, nilai ambang pendengaran, dan tekanan darah. Jumlah sampel yaitu 25 orang yang berkerja di game center. Analisis data dilakukan dengan menggunakan uji Anova, dengan tingkat kemaknaan P <0,1, untuk mengetahui pengaruh kebisingan terhadap tekanan darah. Hasil penelitian menunjukkan adanya gangguan pendengaran pada 60% responden yang bekerja 7-8 jam/hari, dan pada 100% responden yang bekerja >8 jam/hari. Tidak ada nilai korelasi bermakna antara kebisingan dan tekanan darah. Simpulan: Kebisingan dapat menyebabkan gangguan pendengaran, tetapi tidak ditemukan korelasi antara kebisingan dan tekanan darah pada pekerja game center di kotaManado.. Kata kunci: kebisingan, tekanan darah, ketulian.


e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Jizel Zarra ◽  
Krista V. Siagian ◽  
Juliatri .

Abstract: Gingiva is prone to any disturbances. Poor dental and oral health can cause inflammation of the gingiva. Children aged 14-15 years have already permanent teeth that had fully grown. This study was aimed to obtain the gingival status of children aged between 14-15 years old living in the highland and at the coastal area. This was a descriptive observational study with a cross sectional design. The study population was all students aged 14-15 years at SMP GMIM Rurukan and SMP PGRI Tandurusa (Junior high schools). Subjects were 30 students obtained by using purposive sampling. Data of gingival status were obtained from examination of gingival index according to Loe and Silness. The results showed that the gingival status of subjects living in the highland were mild inflammatory category (66.7%) and moderate inflammatory category (33.3%), whereas of those at the coastal area were mild inflammatory category (93.4%) and moderate inflammatory category (3.3%). Keywords: gingival status, children 14-15 years, highland, coast Abstrak: Gingiva merupakan jaringan yang rentan terhadap berbagai gangguan. Buruknya kesehatan gigi dan mulut dapat menimbulkan peradangan gingiva. Pada kelompok usia 14-15 tahun anak telah memiliki gigi permanen yang sudah tumbuh lengkap dalam rongga mulut. Penelitian ini bertujuan untuk mengetahui status gingiva anak usia 14-15 tahun di daerah dataran tinggi dan di daerah pesisir pantai. Jenis penelitian ialah deskriptif observasional dengan desain potong lintang. Populasi penelitian ialah seluruh siswa berusia 14-15 tahun yang bersekolah di SMP GMIM Rurukan dan SMP PGRI Tandurusa. Subjek penelitian diambil sebanyak 30 siswa menggunakan purposive sampling. Pengumpulan data menggunakan formulir pemeriksaan status gingiva berdasarkan indeks gingiva Loe and Silness. Hasil penelitian mendapatkan status gingiva subjek di daerah dataran tinggi tergolong kategori inflamasi ringan (66,7%) dan kategori inflamasi sedang (33,3%), sedangkan status gingiva subjek di pesisir pantai tergolong kategori inflamasi ringan (93,4%) dan kategori inflamasi sedang (3,3%). Kata kunci: Status gingiva, usia 14-15 tahun, dataran tinggi, pesisir pantai


2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Piere Tendean ◽  
Lydia Tendean ◽  
Benny Wantouw

Abstract: Spermiogram is an examination for determination of male fertility. Evaluation of a spermiogram consists of macroscopical and microscopical examination of sperm cells, which are concentration, motility and morphology of sperm cells. This study aimed to obtain the spermiogram of infertile patients with varicocele. This was an observational study with a cross-sectional design by using sperm samples of 30 infertile patients with varicocele. Evaluation of spermatozoa quaity was determined by using WHO standard 2010. The results showed that the sperm concentrations were <15 million/ml, sperm motility <40% /field, and sperm morphology <30%/field. Conclusion: In this study the spermiogram of infertile patients with varicocele was abnormal with oligozoospermi, asthenozoospermi, and teratozoospermi.Keywords: varicocele, spermiogramAbstrak: Spermiogram merupakan salah satu pemeriksaan untuk menentukan fertilitas seorang pria. Evaluasi spermiogram meliputi makroskopik dan mikroskopik sel spermatozoa yaitu konsentrasi, motilitas, dan morfologi sel spermatozoa. Penelitian ini bertujuan untuk mendapatkan gambaran spermiogram penderita infertil dengan varikokel. Penelitian ini bersifat observasional dengan desain potong lintang dengan menggunakan sampel sperma dari 30 penderita infertil dengan varikokel. Evaluasi kualitas spermatozoa berdasarkan standard WHO 2010. Hasil penelitian ini didapatkan yaitu konsentrasi sperma <15 juta/ml, motilitas sperma <40%/lp, dan morfologi sperma < 30%/lp. Simpulan: Pada penelitian ini gambaran spermiogram penderita infertil dengan varikokel ialah abnormal dengan oligozoospermi, asthenozoospermi, dan teratozoospermi. Kata kunci: varikokel, spermiogram


2020 ◽  
Vol 5 (1) ◽  
pp. 195
Author(s):  
Yunita Armiyanti ◽  
Nisrina Salsabila Firmansyah ◽  
Angga Mardro Raharjo ◽  
Muhammad Ali Shodikin ◽  
Enny Suswati ◽  
...  

ABSTRACT Tuberculosis is one of the world's health problems even in Indonesia. The immune response that needed to deal with TB can be influenced by other infections, such as helminth infection. The helminth infections induce Th2 immune responses and eosinophilia to eliminate these pathogen. Meanwhile, the Th2 response and eosinophilia can also suppress the Th1 immune response which is very important to eliminate the bacterium M. tuberculosis and make a negative impact on the success of TB therapy. Thus, the eosinophil profile can describe the immune response in TB patients with helminth co-infection. This study aims to determine the prevalence of helminthiasis in TB patients and determine the differences in eosinophil amount in tuberculosis patients with and without helminth co-infection. This study was an observational analytic study with a cross-sectional design conducted at Puskesmas Panti in August 2019-January 2020 using fecal and blood samples from 24 research subjects. Helminth co-infection status was obtained from fecal examination with sedimentation and flotation methods while the number of eosinophils was obtained from leukocyte counts. Data analysis used the Mann Whitney U test to determine differences in the number of eosinophils in the two groups. The results showed there was no difference in the number of eosinophils in TB patients with helminth co-infection and without helminth co-infection (p> 0.05). There is no typical picture of the number of eosinophils in both groups so that eosinophils cannot describe the immune response that arose in TB patients with helminth co-infections in Panti district, Jember.


e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Yuniar P. Panjaitan ◽  
Shane H. R. Ticoalu ◽  
Krista V. Siagian

Abstract: Full dentures are made for people with missing teeth entirely. One of the objectives of full denture usage is to improve and enhance the function of mastication. Mastication is a process of pulverizing food to be swallowed and digested. This study aimed to assess the mastication ability of patients with full denture made at Dental Hospital of the University of Sam Ratulangi Manado. This was a descriptive observational study with a cross sectional design. There were 56 respondents in this study obtained by using total sampling method. Respondents were patients with full dentures made at the Dental Hospital University of Sam Ratulangi during January 2014 - December 2015. The instrument consisted of questionnaires about mastication ability. Data were processed and analyzed descriptively then were presented in tables. The results showed that 60.71% of respondents had good ability of mastication. Conclusion: The mastication ability of patients with full denture at the Dental Hospital of the University of Sam Ratulangi was in good categoryKeywords: full denture, mastication, the ability of masticationAbstrak: Gigi tiruan penuh dibuat untuk penderita kehilangan gigi seluruhnya yang bertujuan antara lain untuk memperbaiki dan meningkatkan fungsi mastikasi. Mastikasi merupakan proses melumatkan makanan untuk ditelan dan dicerna. Penelitian ini bertujuan untuk menilai kemampuan mastikasi pasien pengguna gigi tiruan penuh di Rumah Sakit Gigi dan Mulut Universitas Sam Ratulangi Manado. Jenis penelitian ialah deskriptif observasional dengan desain potong lintang. Terdapat 56 responden yang diperoleh dengan metode total sampling yaitu pasien yang membuat gigi tiruan penuh di Rumah Sakit Gigi dan Mulut Universitas Sam Ratulangi periode Januari 2014-Desember 2015. Instrumen penelitian berupa kuesioner mengenai kemampuan mastikasi. Data yang diperoleh diolah dan dianalisis secara deskriptif kemudian disajikan berdasarkan distribusi dalam bentuk tabel. Hasil penelitian menunjukkan bahwa 60,71% responden memiliki kemampuan mastikasi yang baik. Simpulan: Kemampuan mastikasi pasien pengguna gigi tiruan penuh di Rumah Sakit Gigi dan Mulut Universitas Sam Ratulangi dalam kategori baik.Kata kunci: gigi tiruan penuh, mastikasi, kemampuan mastikasi


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