scholarly journals PENGAMATAN PERTUMBUHAN TANAMAN BAYAM (Amaranthus tricolor L.) PASCA APLIKASI BIOFERTILIZER (BAHAN AKTIF Aspergillus sp.) SEDIAAN CAIR

Biocelebes ◽  
2020 ◽  
Vol 14 (2) ◽  
pp. 199-209
Author(s):  
Yulia Astuti ◽  
Umrah ◽  
Abdul Rahim Thaha

The study about the observation of spinach (Amaranthus tricolor L.) growth after the addition of liquid biofertilizer (active ingredient Aspergillussp.) had been conducted in Taman Pangan Gizi Langaleso Village, Dolo Subdistrict, Sigi District, Central Sulawesi, and Laboratory of Biotechnology, Biology Department, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu, from March to November 2018. The aim of the study was to observe the growth of spinach after the addition liquid biofertilizer (active ingredient Aspergillus sp.) along with the fermented soybean liquid waste and coconut water waste as the main media. This study was designed using the Completely Randomized Design which consisted of 7 treatments with 5 repetitions. The treatments were arranged as follows: P0 (without the addition ofbiofertilizer, as the negative control), P1 (2.5%biofertilizer addition), P2 (5%biofertilizer addition), P3 (7.5%biofertilizer addition), P4 (10%biofertilizer addition), P5 (12.5%biofertilizer addition), P6 (NPK addition, without the addition of biofertilizer, acting as the positive control). The results showed that treatment P4 was indicated as the best growth among any other treatments as well as the control, based on the parameters of the height of the plants (10 cm), the number of leaves (8.35 leaf blades),  the dry weight of biomass (1.95 g), the fresh weight of canopy (4.94), the fresh weight of leaves (2.07 g), the dry weight of leaves (0.22 g), the fresh weight of roots (2.11 g), the dry weight of roots (0.24 g) and the length of the roots (12.2 cm).

Biocelebes ◽  
2020 ◽  
Vol 14 (3) ◽  
pp. 292-302
Author(s):  
Miranty Sirappa ◽  
Umrah ◽  
Abdul Rahim Thaha

Liquid Organic fertilizer is a fertilizer produced from organic materials in the form of liquid preparations. Research on the application of liquid organic fertilizer (active ingredient Aspergillus sp.) is based on liquid waste of Tempe and coconut water on the Kangkung land plant (Ipomoea reptans Poir.), this research has been conducted in March to November 2018 in the village Langaleso, Dolo subdistrict, Sigi Regency, Biotechnology Laboratory of Biology Department of Mathematics and Natural Sciences of Tadulako University. This research aims to observe the growth of plant Kangkung post application of liquid organic fertilizer (active ingredient Aspergillus sp.). The research designed in the complete random plan (RAL) consists of seven types and five times the replay. Composition of treatment; P0 (without the application of POC, control), P1 (Application of POC 2.5%), P2 (Application of POC 5%), P3 (Application of POC 7.5%), P4 (Application of POC 210%), P5 (Application of POC 12.5%), P6 (NPK application, without POC). Description POC Liquid Organic fertilizer (active ingredient Aspergillus sp.). The observation results showed the best growth on the implementation of P1 based on the fresh weight of the heading (8.47 g), the fresh weight of biomass (6.02 g), the fresh weight of the leaves (4.66 strands), the fresh weight of the roots (5.72 g). The P2 is based on the root length (27.10 cm). P4 treatment based on the high average plant (27.34 cm), the number of leaves (14.90 strands), dry weight of the roots (1.61 g).  P6 treatment based on leaf dry weight (2.07 g).


2020 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Icha Rusita ◽  
Hadi Sasongko

Moler disease is the primary disease that disturbs the shallot’ cultivation. Hence, it is beneficial to have preventive measures to mitigate the risk and to improve growth. The research objective is to know the effectivity of Trichoderma harzianum suspension as a bio-fungicide and bio-stimulator. The study used seven treatments, i.e., immersion of shallot seeds on negative control (distilled water), positive control (synthetic fungicide+ZPT 1g/liter), T.harzianum suspension with different concentrations of 2 ppm, 4 ppm, 6 ppm, 8 ppm, and 10 ppm, assigned in a Completely Randomized Design (CRD). Treatment that results in a significant level will be continued to the Least Significance Different (LSD) 5% test. The observed variables are the intensity of moler disease and the growth of shallot seeds. Experiment results show the significant difference between treatments. The optimal treatment to reduce the percentage of moler disease intensity is the immersion of shallot seeds in 10 ppm-concentrated T.harzianum suspension, while to improve the shallot growth is in 8 ppm-concentrated T.harzianum suspension. The intensity of moler disease has a negative correlation to the plant’s height and leaf’s diameter but shows no correlation pattern to the number of leaves, wet weight, dry weight, and length of roots.


2020 ◽  
Vol 3 (2) ◽  
pp. 17-24
Author(s):  
Muhammad Habibullah ◽  
Danes Suhendra

Introduction: Downy mildew control using pesticides has been used intensively and led to the onset of resistant strains. This research aims to find out the effect of bamboo vinegar administration on disease incidence, disease severity, disease progression, and growth of maize. Materials and Methods: This research was conducted from June to August 2020 in the laboratory and Screenhouse of the Faculty of Agriculture, Universitas Tidar. The experiment was experimentally conducted using a Completely Randomized Design (CRD) consisting of 3 treatments with 6. are plants. In each polybag used there are 3 maize so there are 54 plants tested. The treatment tested is of bamboo vinegar and maydis (negative control), of bamboo vinegar with inoculation P. maydis (positive control), application bamboo vinegar 2 ml l-1 with inoculation P. maydis. Observations on the agronomic influence of maize covering height, , and dry weight were analyzed using ANOVA at a significance of 5% followed by LSD test at the same real level using software R v. 4.0.2. Results: Bamboo vinegar showed lignification in the treatment of stronger bamboo vinegar in corn leaf tissue, did not decrease in disease incidence, but decreased the disease severity by 22.18% and decreased disease the progression by 34.74% based on AUDPC value. The growth of maize, bamboo vinegar-treated shows values are not significant negative control.


2020 ◽  
Vol 9 (9) ◽  
pp. e266996912
Author(s):  
Sara Beatriz da Costa Santos ◽  
Solange de Sousa ◽  
Vênia Camelo de Souza ◽  
George Rodrigo Beltrão da Cruz ◽  
Thiago de Sousa Melo ◽  
...  

This work compares the productivity and postharvest quality of C. sativum produced in organic and conventional systems. We used a randomized block experiment with five treatments and six repetitions each. Treatments comprised a negative control (C-: without fertilization), three doses of bovine manure as organic fertilization (35, 70, and 105 kg of N ha -1, named T35, T70, and T105 respectively), and a positive control (C+: 70 Kg of N ha -1 of conventional fertilization). We evaluated the morphometry, productivity, and physical-chemical characteristics of all plants used. The effect of organic fertilizer doses was analyzed using polynomial regressions. The differences among negative control, the positive control, and the organic fertilization with 70 Kg of N ha -1 of manure were tested with orthogonal contrasts. The doses of N used in organic fertilization influence the water and protein activity of C. Sativum leaves, promoting their decrease. The contrasts influenced the productivity, total fresh weight, fresh weight of the aerial part, dry weight of the aerial part, dry weight of the root, the total dry weight, water activity, titratable acidity, soluble solids, carotenoids, total chlorophyll, chlorophyll b, C. sativum was influenced by comparisons. The conventional production system using ammonium sulfate as the source of nitrogen promoted higher productivity and better postharvest quality in the culture of C. sativum. The period of implementation of bovine manure hampered the organic system. We suggest the fertilization with bovine manure before sowing, providing the necessary time for its mineralization during the cultivation cycle.


2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.


2020 ◽  
Vol 9 (1) ◽  
pp. 706-714
Author(s):  
Muhammad Ikram ◽  
Naveed Iqbal Raja ◽  
Bilal Javed ◽  
Zia-ur-Rehman Mashwani ◽  
Mubashir Hussain ◽  
...  

AbstractThe present study was aimed to biosynthesize selenium nanoparticles (SeNPs) and assess their foliar applications to improve the growth of wheat plants under controlled irrigation and drought stress. Bud aqueous extract of Allium sativum L. was used as a reducing and stabilizing agent of SeNPs followed by their optical and morphological characterization by using ultraviolet-visible spectroscopy, scanning electron microscopy, Fourier-transform infrared, and energy dispersive X-ray analysis. Various concentrations of SeNPs (10, 20, 30, and 40 mg/L) were applied exogenously to drought-tolerant (V1) and drought-susceptible (V2) wheat varieties at the trifoliate stage. Under the positive control conditions, plants were irrigated with 450 mL of water/pot (100% field capacity); and under water-deficit environment, plants were irrigated with 160 mL of water/pot (35% field capacity). Remarkable increase in plant height, shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, leaf area, leaf number, and leaf length has been observed when 30 mg/L concentration of SeNPs was used. However, the plant morphological parameters decreased gradually at higher concentrations (40 mg/L) in both selected wheat varieties. Therefore, 30 mg/L concentration of SeNPs was found most preferable to enhance the growth of selected wheat varieties under normal and water-deficient conditions.


PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 736
Author(s):  
Grace Laury Tulung ◽  
Widdhi Bodhi ◽  
Jainer Pasca Siampa

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords:  Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus  ABSTRAK  Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus


2020 ◽  
Vol 11 (1) ◽  
pp. 25
Author(s):  
AHMAD TAOFIK ◽  
SOFIYA HASANI ◽  
AYU CAHYANINGTYAS ◽  
BUDY FRASETYA

The paitan plant contains allelopathic compounds for other plants so that the utilize of paitan plant as a source of liquid organic fertilizer (LOF) has not been widely applied. This research aimed to study the interaction between LOF dosage and appropriate application method of LOF to increase the growth of kailan plants. The research conducted at experimental garden of UIN Bandung from June-August 2016. The experimental design was a completely randomized design with two factors and replicated four times. The first factor was application methods (A), i.e. a1=direct application to the soil, and a2= spraying. The second factor was LOF dosage (T), i.e. t0: 0 ml plant-1, t1: 20 ml plant-1, t2: 40 ml plant-1, t3: 60 ml plant-1, t4: 80 ml plant-1. The growth parameters observed i.e. plant height (14, 21, 28, 35 and 42 Day after Transplanting (DAT)), leaf area 42 DAT, plant dry weight 42 DAT, and plant fresh weight 42 DAT. The data then analyzed with analysis of variance at a significant level of 5%. If the analysis of variance was significant, the Duncan multiple range test was used at α=5%. The research results showed there was no interaction between method of application with a various dosage of paitan LOF to all of the growth parameters. The application paitan LOF showed similar growth in terms of plant height, leaf area, plant dry, and plant fresh weight. The application method of paitan LOF direct to the soil or spraying to leaves can not increase kailan plant growth.


2016 ◽  
Vol 16 (2) ◽  
pp. 76
Author(s):  
Wasir Ibrahim ◽  
Rita Mutia ◽  
Nurhayati Nurhayati ◽  
Nelwida Nelwida ◽  
Berliana Berliana

ABSTRAK. Penelitian ini bertujuan untuk mengetahui penggunaan kulit nanas fermentasi ke dalam ransum yang mengandung gulma berkhasiat obat terhadap konsumsi nutrient ayam broiler. Penelitian ini menggunakan DOC jantan strain cob umur 2 hari sebanyak 200 ekor yang dipelihara selama 42 hari. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. P0 = 0% tepung kulit nanas fermentasi (TKNF) dalam ransum mengandung 0% gulma obat(GO), (kontrol positif), P1 = 0% TKNF dalam ransum mengandung 2 % gulma obat (GO),(kontrol negatif), P2 = 7.5% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat, P3 = 15% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat, P4 = 22.5% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat. Parameter yang diamati kandungan gizi kulit nanas sebelum dan sesudah fermentasi dan konsumsi nutrient. Pengaruh yang nyata terhadap parameter yang diamati dilanjutkan dengan Uji Jarak Berganda Duncan, Data yang diperoleh dari setiap parameter dianalisis ragam (ANOVA) menggunakan bantuan software SPSS 16.0. Dari hasil penelitian disimpulkan bahwa penggunaan kulit nanas yang fermentasi dengan yoghurt dalam ransum yang mengandung gulma berkhasiat obat pada taraf 15% memberikan hasil terbaik pada konsumsi nutrient ayam broiler. (Fermented pineapple peel supplementation with addition of medicinal weeds on nutrient intake consumption of broiler chicken) ABSTRACT. The aim of this study was to determine the effect of using fermented pineapple peel in the ration containing medicinal weeds on nutrient consumption and performance of broiler . Two hundred 2 days male broiler chicken cob strain were used in this study and kept for 42 days. Completely randomized design (CRD)was applied with 5 treatments and 5 replicates. Treatments consisted of P0 = 0% of fermented pineapple peels meal (FPPM) in ration containing 0% medicinal weed (MW), positive control). P1 = 0% of fermented pineapple peels meal (FPPM) in the ration containing 2 % medicinal weed (MW), negative control). P2 = 7.5% of FPPM in the ration containing 2 % medicinal weed (MW). P3 = 15% of FPPM in the ration containing 2 % medicinal weed (MW). P4 = 22,5% of FPPM in the ration containing 2 % medicinal weed (MW). The parameters measured was nutrien intake before and after fermented peel and consumption. The observed data were analyzed by statistical product and service solution (SPSS 16.0). Results showed that use of fermented pineapple peel meal up to 15% with addition medicinal weeds was the best on nutrient consumption of broiler chicken.


Agrikultura ◽  
2019 ◽  
Vol 30 (1) ◽  
pp. 17
Author(s):  
Sri Hartati ◽  
Linda Tarina ◽  
Endah Yulia ◽  
Luciana Djaya

ABSTRACTEffect of induced resistance by Candida tropicalis on the growth of chili plants infected by Colletotrichum acutatumColletotrichum acutatum is a major pathogen of chili that causes a big yield loss. Candida tropicalis has been reported as a biocontrol agent antagonistic to C. acutatum. The research was objected tofind out the effect of induced resistance by C. tropicalis on the growth of chili plants infected by C. acutatum. The application of the yeast was carried out by seed dipping and seedling spraying methods. As treatments, C. acutatum, the pathogen was inoculated at different time, i.e. A = inoculation at 3 days after the induction (dai) by C. tropicalis, B = inoculation at 7 dai, C = inoculation at 10 dai, D = positive control on inoculation at 3 dai, E = positive control on inoculation at 7 dai, F = positive control on inoculation at 10 dai, G = negative control with no induction nor inoculation. The results showed that the induction by C. tropicalis was able to increase the plant height, leaf number, and dry weight. The highest increase of plant height of 30.72% was caused by induction of C. tropicalis inoculated at 7 dai. Induction of C. tropicalis inoculated at 3 dai increased the leaf number by 49.47%, and the plant dry weight by 50%. C. tropicalis has a potency to be developed as plant growth inducer.Keywords: Antagonist, Inoculation, Fungi, YeastABSTRAKJamur Colletotrichum acutatum merupakan patogen penyebab kehilangan hasil panen yang cukup besar pada tanaman cabai. Salah satu mikrob antagonis jamur ini yang telah dilaporkan adalah khamir Candida tropicalis. Penelitian ini bertujuan untuk mengetahui pengaruh induksi resistensioleh C. tropicalis terhadap pertumbuhan tanaman cabai terinfeksi C. acutatum. Penelitian dilaksanakan dengan menggunakan teknik perendaman benih dan penyiraman bibit tanaman cabaimenggunakan suspensi C. tropicalis dengan waktu inokulasi patogen C. acutatum yang berbeda. Perlakuan tersebut adalah A = Inokulasi C. acutatum 3 hari setelah perlakuan induksi C. tropicalis (hsp), B = Inokulasi C. acutatum 7 hsp, C = Inokulasi C. acutatum 10 hsp, D = Kontrol (+) dengan inokulasi C. acutatum 3 hsp, E = Kontrol (+) dengan inokulasi C. acutatum 7 hsp, F = Kontrol (+) dengan inokulasi C. acutatum 10 hsp, G = Kontrol (-) tanpa induksi dan inokulasi. Hasil penelitianmenunjukkan bahwa perlakuan induksi resistensi oleh khamir C. tropicalis mampu meningkatkan tinggi, jumlah daun, dan bobot kering tanaman cabai. Kemampuan meningkatkan tinggi tanamancabai tertinggi terjadi pada perlakuan C. tropicalis dengan waktu inokulasi 7 hsp yaitu sebesar 30,72%. Perlakuan khamir C. tropicalis dengan inokulasi patogen 3 hsp mampu meningkatkan jumlah daun tanaman cabai sebesar 49,47%. Perlakuan C. tropicalis dengan waktu inokulasi 3 hsp mampu meningkatkan bobot kering tanaman sebesar 50%. Khamir C. tropicalis berpotensi untuk dikembangkan sebagai agens pemacu pertumbuhan tanaman.Kata Kunci: Antagonis, Inokulasi, Jamur, Patogen


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