scholarly journals Three-dimensional printing of cell-laden bioink for blood vessel tissue engineering: Influence of process parameters and components on cell viability

2021 ◽  
Author(s):  
CONGCONG ZHAN ◽  
Yasong Hu ◽  
ANDUO ZHOU ◽  
SHANFENG ZHANG ◽  
Xia Huang
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Cell Viability ◽  
Process Parameters ◽  
Umbilical Vein ◽  
Three Dimensional ◽  
Small Diameter ◽  
3D Bioprinting ◽  
Influence Of Process Parameters ◽  
Composite Gels

Three-dimensional (3D) bioprinting is a potential therapeutic method for tissue engineering owing to its ability to prepare cell-laden tissue constructs. The properties of bioink are crucial to accurately control the printing structure. Meanwhile, the effect of process parameters on the precise structure is not nonsignificant. We investigated the correlation between process parameters of 3D bioprinting and the structural response of κ-carrageenan-based hydrogels to explore the controllable structure, printing resolution, and cell survival rate. Small-diameter (<6 mm) gel filaments with different structures were printed by varying the shear stress of the extrusion bioprinter to simulate the natural blood vessel structure. The cell viability of the scaffold was evaluated. The in vitro culture of human umbilical vein endothelium cells (HUVECs) on the κ-carrageenan (kc) and composite gels (carrageenan/carbon nanotube and carrageenan/sodium alginate) demonstrated that the cell attachment and proliferation on composite gels were better than those on pure kc. Our results revealed that the carrageenan-based composite bioinks offer better printability, sufficient mechanical stiffness, interconnectivity, and biocompatibility. This process can facilitate precise adjustment of the pore size, porosity, and pore distribution of the hydrogel structure by optimising the printing parameters as well as realise the precise preparation of the internal structure of the 3D hydrogel-based tissue engineering scaffold. Moreover, we obtained perfused tubular filament by 3D printing at optimal process parameters.

scholarly journals Current Advances in 3D Bioprinting Technology and Its Applications for Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2958
Author(s):  
JunJie Yu ◽  
Su A Park ◽  
Wan Doo Kim ◽  
Taeho Ha ◽  
Yuan-Zhu Xin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Cell Density ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Living Cells ◽  
3D Bioprinting ◽  
3D Objects ◽  
Advantages And Disadvantages ◽  
Resolution Cell

Three-dimensional (3D) bioprinting technology has emerged as a powerful biofabrication platform for tissue engineering because of its ability to engineer living cells and biomaterial-based 3D objects. Over the last few decades, droplet-based, extrusion-based, and laser-assisted bioprinters have been developed to fulfill certain requirements in terms of resolution, cell viability, cell density, etc. Simultaneously, various bio-inks based on natural–synthetic biomaterials have been developed and applied for successful tissue regeneration. To engineer more realistic artificial tissues/organs, mixtures of bio-inks with various recipes have also been developed. Taken together, this review describes the fundamental characteristics of the existing bioprinters and bio-inks that have been currently developed, followed by their advantages and disadvantages. Finally, various tissue engineering applications using 3D bioprinting are briefly introduced.

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

Hydrogel-Based Bioinks for Three-Dimensional Bioprinting: Patent Analysis

2021 ◽  
Vol 7 (1) ◽  
pp. 3
Author(s):  
Ahmed Fatimi
Keyword(s):  
Tissue Engineering ◽  
Detailed Analysis ◽  
State Of The Art ◽  
Three Dimensional ◽  
Patent Analysis ◽  
The State ◽  
Driving Factors ◽  
3D Bioprinting ◽  
Preparation Methods

There are a variety of hydrogel-based bioinks commonly used in three-dimensional bioprinting. In this study, in the form of patent analysis, the state of the art has been reviewed by introducing what has been patented in relation to hydrogel-based bioinks. Furthermore, a detailed analysis of the patentability of the used hydrogels, their preparation methods and their formulations, as well as the 3D bioprinting process using hydrogels, have been provided by determining publication years, jurisdictions, inventors, applicants, owners, and classifications. The classification of patents reveals that most inventions intended for hydrogels used as materials for prostheses or for coating prostheses are characterized by their function or properties Knowledge clusters and expert driving factors show that biomaterials, tissue engineering, and biofabrication research is concentrated in the most patents.

scholarly journals Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering

Materials ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 3522
Author(s):  
Su Jeong Lee ◽  
Jun Hee Lee ◽  
Jisun Park ◽  
Wan Doo Kim ◽  
Su A Park
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Research Groups ◽  
Porcine Skin ◽  
Alginate Hydrogel ◽  
Chemical Treatments ◽  
Soft Tissue Engineering

Recently, many research groups have investigated three-dimensional (3D) bioprinting techniques for tissue engineering and regenerative medicine. The bio-ink used in 3D bioprinting is typically a combination of synthetic and natural materials. In this study, we prepared bio-ink containing porcine skin powder (PSP) to determine rheological properties, biocompatibility, and extracellular matrix (ECM) formation in cells in PSP-ink after 3D printing. PSP was extracted without cells by mechanical, enzymatic, and chemical treatments of porcine dermis tissue. Our developed PSP-containing bio-ink showed enhanced printability and biocompatibility. To identify whether the bio-ink was printable, the viscosity of bio-ink and alginate hydrogel was analyzed with different concentration of PSP. As the PSP concentration increased, viscosity also increased. To assess the biocompatibility of the PSP-containing bio-ink, cells mixed with bio-ink printed structures were measured using a live/dead assay and WST-1 assay. Nearly no dead cells were observed in the structure containing 10 mg/mL PSP-ink, indicating that the amounts of PSP-ink used were nontoxic. In conclusion, the proposed skin dermis decellularized bio-ink is a candidate for 3D bioprinting.

Design and additive manufacturing of porous titanium scaffolds for optimum cell viability in bone tissue engineering

2020 ◽  
pp. 095440542093756
Author(s):  
Bingbing Li ◽  
Bani Davod Hesar ◽  
Yiwen Zhao ◽  
Li Ding
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Bone Tissue Engineering ◽  
Pore Size ◽  
Bone Tissue ◽  
Structural Strength ◽  
Three Dimensional ◽  
Porous Titanium ◽  
Nih3t3 Cells ◽  
Pore Sizes

Pore size, external shape, and internal complexity of additively manufactured porous titanium scaffolds are three primary determinants of cell viability and structural strength of scaffolds in bone tissue engineering. To obtain an optimal design with the combination of all three determinants, four scaffolds each with a unique topology (external geometry and internal structure) were designed and varied the pore sizes of each scaffold 3 times. For each topology, scaffolds with pore sizes of 300, 400, and 500 µm were designed. All designed scaffolds were additively manufactured in material Ti6Al4V by the direct metal laser melting machine. Compression test was conducted on the scaffolds to assure meeting minimum compressive strength of human bone. The effects of pore size and topology on the cell viability of the scaffolds were analyzed. The 12 scaffolds were ultrasonically cleaned and seeded with NIH3T3 cells. Each scaffold was seeded with 1 million cells. After 32 days of culturing, the cells were fixed for their three-dimensional architecture preservation and to obtain scanning electron microscope images.

scholarly journals A Customizable, Low-Cost Perfusion System for Sustaining Tissue Constructs

2018 ◽  
Vol 23 (6) ◽  
pp. 592-598
Author(s):  
Brian J. O’Grady ◽  
Jason X. Wang ◽  
Shannon L. Faley ◽  
Daniel A. Balikov ◽  
Ethan S. Lippmann ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Large Scale ◽  
Low Cost ◽  
Three Dimensional ◽  
Tissue Scaffolds ◽  
Perfusion System ◽  
Pump System ◽  
Tissue Constructs ◽  
Engineered Tissue

The fabrication of engineered vascularized tissues and organs requiring sustained, controlled perfusion has been facilitated by the development of several pump systems. Currently, researchers in the field of tissue engineering require the use of pump systems that are in general large, expensive, and generically designed. Overall, these pumps often fail to meet the unique demands of perfusing clinically useful tissue constructs. Here, we describe a pumping platform that overcomes these limitations and enables scalable perfusion of large, three-dimensional hydrogels. We demonstrate the ability to perfuse multiple separate channels inside hydrogel slabs using a preprogrammed schedule that dictates pumping speed and time. The use of this pump system to perfuse channels in large-scale engineered tissue scaffolds sustained cell viability over several weeks.

scholarly journals Vascular Tissue Engineering: Recent Advances in Small Diameter Blood Vessel Regeneration

2014 ◽  
Vol 2014 ◽  
pp. 1-27 ◽  
Author(s):  
Valentina Catto ◽  
Silvia Farè ◽  
Giuliano Freddi ◽  
Maria Cristina Tanzi
Keyword(s):  
Tissue Engineering ◽  
Cardiovascular Diseases ◽  
Blood Vessel ◽  
Polyethylene Terephthalate ◽  
Vascular Tissue ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Synthetic Polymers ◽  
Vascular Tissue Engineering ◽  
Vessel Regeneration

Cardiovascular diseases are the leading cause of mortality around the globe. The development of a functional and appropriate substitute for small diameter blood vessel replacement is still a challenge to overcome the main drawbacks of autografts and the inadequate performances of synthetic prostheses made of polyethylene terephthalate (PET, Dacron) and expanded polytetrafluoroethylene (ePTFE, Goretex). Therefore, vascular tissue engineering has become a promising approach for small diameter blood vessel regeneration as demonstrated by the increasing interest dedicated to this field. This review is focused on the most relevant and recent studies concerning vascular tissue engineering for small diameter blood vessel applications. Specifically, the present work reviews research on the development of tissue-engineered vascular grafts made of decellularized matrices and natural and/or biodegradable synthetic polymers and their realization without scaffold.

Spatiotemporal Intracellular Deformation of Cells During Freezing-Induced Cell-Fluid-Matrix Interactions

2013 ◽  
Author(s):  
Soham Ghosh ◽  
J. Craig Dutton ◽  
Bumsoo Han
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Three Dimensional ◽  
Tissue Type ◽  
Major Constituent ◽  
Tissue Banks ◽  
Chemical Additives ◽  
Cryoprotective Agents ◽  
Cell Tissue ◽  
The Status

Freezing of biomaterials is emerging as one of the key biotechnologies in cell/tissue engineering, medicine and biology. Its applications include — 1) preservation of cell/tissue engineering products, 2) quality control of biospecimens cryopreserved in tissue banks and repositories, and 3) synthesis procedures of biomaterials such as decellularization of native tissues to create acellular (i.e., cell-free) complex three-dimensional extracellular matrices (ECMs). Traditionally, research efforts have focused on determining optimal freeze/thaw (F/T) protocols with chemical additives, so called cryoprotective agents, for a given cell/tissue-type by comparing the outcomes of F/T protocols, which are mainly gauged by cell viability. Although cell viability is the major constituent, it has recently been recognized that other features beyond viability are also critical to the functionality of biomaterials, including the microstructure of the ECM, the status of cell-matrix adhesion, and the cytoskeletal structure and organization [1, 2, 3].

The Fabrication of Tissue Engineering Scaffolds by Inkjet Printing Technology

2018 ◽  
Vol 934 ◽  
pp. 129-133 ◽  
Author(s):  
Chao Fan Lv ◽  
Li Ya Zhu ◽  
Jian Ping Shi ◽  
Zong An Li ◽  
Wen Lai Tang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sodium Alginate ◽  
Inkjet Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Tissue Engineering Scaffolds ◽  
Printing Technology ◽  
3D Printed ◽  
Inkjet Printing Technology ◽  
Alginate Scaffold

Three-dimensional (3D) printing has been playing an important role in diverse areas in medicine. In order to promote the development of tissue engineering, this study attempts to fabricate tissue engineering scaffolds using the inkjet printing technology. Sodium alginate, exhibiting similar properties to the native human extracellular matrix (ECM), was used as bioink. The jetted fluid of sodium alginate would be gelatinized when printed into the calcium chloride solution. The characteristics of the 3D-printed sodium alginate scaffold were systematically measured and analyzed. The results show that, the pore size, porosity and degradation property of these scaffolds could be well controlled. This study indicates the capability of 3D bioprinting technology for preparing tissue engineering scaffolds.

Electrospun tecophilic/gelatin nanofibers with potential for small diameter blood vessel tissue engineering

Biopolymers ◽  
2014 ◽  
Vol 101 (12) ◽  
pp. 1165-1180 ◽  
Author(s):  
Elham Vatankhah ◽  
Molamma P. Prabhakaran ◽  
Dariush Semnani ◽  
Shahnaz Razavi ◽  
Mohammad Morshed ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Small Diameter
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