scholarly journals Essential oil and ethanolic extract composition from Myrtus nivellei Batt. & Trab. and their biological evaluations

2021 ◽  
pp. 111-121
Author(s):  
Farah Ramdane ◽  
Oualid Medjour ◽  
Aoun Ben ◽  
Soumia Hadjadj ◽  
Messouda Guemouda ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biological Effects ◽  
Condensed Tannin ◽  
Ethanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Antibacterial Screening ◽  
Aerial Parts ◽  
Colorimetric Methods

This study aims to evaluate the chemical composition and biological effects of an endemic Algerian species Myrtus nivellei belonging to the Myrtaceae family. The aerial parts of the plant were submitted to hydrodistillation and analysed with gas chromatography - mass spectrometry (GC-MS). This oil was yielded 0,75% (w/w). The GC-MS revealed that 1,8 cineole is the most abundant component (53,44%) while other compounds were present in high contents: 2,2,6,7-tetramethyl-10-oxatricyclo [4.3.0.1(1,7)]- decan-5-one (11,73%), trans ocimene (7,54%), linalyl propirionate (6,81%) and lavandulyl acetate (5,24%). In addition ethanolic extract of studied plant was prepared. Total phenol, tannin, flavonoid, and condensed tannin?s contents were determined using Folin-Ciocalteu, aluminum chloride and vanillin colorimetric methods, respectively. Three tests were used for the investigation of antioxidant activity of both extract and oil. Strong antioxidant activity was shown mainly by crude extract. Antibacterial screening was evaluated against Gram positive and Gram negative bacteria. Ethanolic extract and oil were active especially against Staphylococcus aureus.

scholarly journals In vitro antimicrobial and antioxidant activities of the essential oil of Craniotome furcata

2010 ◽  
Vol 2 (1) ◽  
pp. 57-62 ◽  
Author(s):  
R. K. Joshi
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Radical Cation ◽  
Steam Distillation ◽  
Antioxidant Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Aerial Parts ◽  
Tube Dilution

The aim of present study was, to investigate the essential oil of Craniotome furcata for its antimicrobial and antioxidant activities. The essential oil was obtained by steam distillation of the flowering aerial parts of C. furcata. The oil was tested for its antimicrobial activity in vitro against four Gram-positive, four Gram-negative bacteria and three fungi, by using disk diffusion and tube dilution methods. The antioxidant potential of the essential oil was investigated by two different models DPPH free radical and ABTS free radical cation scavenging. The essential oil was effective against all the tested microorganisms, with minimum inhibitory concentrations (MICs) ranged from 4.11±1.44 to 0.30±0.10 mg/ml. The oil showed antioxidant activity in DPPH free radical and ABTS free radical cation scavenging model. The results of this study revealed that the essential oil of C. furcata possesses antimicrobial and antioxidant activities.

Analysis and comparison of alkaline and acid phosphatases of Gram-negative bacteria by bioinformatic and colorimetric methods

2020 ◽  
Vol 308 ◽  
pp. 56-62
Author(s):  
Masoomeh Amoozadeh ◽  
Mandana Behbahani ◽  
Hassan Mohabatkar ◽  
Mehrnaz Keyhanfar
Keyword(s):  
Gram Negative Bacteria ◽  
Acid Phosphatases ◽  
Gram Negative ◽  
Alkaline And Acid Phosphatases ◽  
Colorimetric Methods

scholarly journals Synthesis and biological activities of some indoline derivatives

2009 ◽  
Vol 74 (12) ◽  
pp. 1377-1387 ◽  
Author(s):  
Milind Rode ◽  
Sahebrao Rindhe ◽  
Bhausaheb Karale
Keyword(s):  
Antioxidant Activity ◽  
Aromatic Amines ◽  
Benzoyl Chloride ◽  
Stannous Chloride ◽  
Biological Activities ◽  
Gram Negative Bacteria ◽  
Good Activity ◽  
Dpph Assay ◽  
Gram Negative

The reaction of indoline with a substituted benzoyl chloride in the presence of K2CO3 in THF gave compound 4. Compound 4 was subjected to chlorosulphonation to obtain compound 5. Condensation of aromatic amines with compound 5 led to the synthesis of indoline derivatives 6(a-f). Similarly, 5-nitroindoline was treated with a substituted benzoyl chloride to obtain the nitro compound 9, which was reduced using stannous chloride and reacted further with aromatic sulphonyl chloride to obtain the indoline derivatives 11(a-e). These compounds were tested for antibacterial, anti-tuberculosis and antifungal activity. Some of them showed very good activity against some gram-positive and gram negative bacteria, fungal strains and also Mycobacterium tuberculosis. All of the synthesized compounds were subjected to antioxidant activity testing using the in vitro DPPH assay and most of them showed very good activity.

scholarly journals A study of antimicrobial activities of aqueous and ethanolic extract of bee pollen against

2021 ◽  
Author(s):  
Kasim Roba Jilo
Keyword(s):  
Medicinal Plants ◽  
Aqueous Extract ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Distilled Water ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Bee Pollen ◽  
Stock Solutions

Abstract Background Ethiopia is one of the plant species-rich countries in the world and the center of origin of many medicinal plants. Studying antimicrobial activities of pollen is vivacious to investigate plant resources for medicinal values and the study was conducted to evaluate antimicrobial properties of bee pollen against mentioned bacteria. Methods Completely Randomized Design was used for laboratory work. After adjusting turbidity, consistent growth of bacterial culture was made using a sterilized cotton swab. 20 grams of bee pollen was added to 200 ml of distilled water as well as ethanol and finally, the extract was filtered by Whatman filter by paper, dried and weighted and stock solutions were made as follows,3.6 gm. was added to 12 ml of distilled water to prepare stock solutions as follows 3.6:12 = 0.3 x \({10}^{6}\) = 3 x \({10}^{5}\) ppm stock solution and antimicrobial activities of pollen were tested against mentioned bacteria. Data were imported to R software version 3.44. Multilevel analysis was used to see the interaction between bacteria species and each concentration of pollen and Anova was used to see the significance of these concentrations on bacteria species. A p-value of < 0.05 was considered statistically significant. Results Results indicated that bacteria were more inhibited at 72 hours than 48 and 24 hours and the results showed an ethanolic extract of bee pollen had antimicrobial activities against both Gram-negative and Gram-positive bacteria mentioned above. Time has significant effects on tested bacteria (p = 0.000) and treatments have significant effects on tested organisms (p = 0.000). The ethanolic extract inhibited the growth of more Gram-negative bacteria: Escherichia coli and Shigella boydii. Bacillus subtilis was mostly inhibited by aqueous extract of bee pollen than others. Conclusions Ethanolic extract of pollen had antibacterial activities against all tested bacterial strains even though it is concentration and time-based. The ethanolic extract inhibited more Gram-negative bacteria relatively while aqueous extract inhibited more Gram-positive relatively. Negative controls (sterilized water) didn’t show any antimicrobial properties, while positive control (Chloramphenicol) had antimicrobial activities. Further isolation and characterization of bioactive compounds from pollen are useful to develop a novel botanical formulation for further applications from the pollen of medicinal plants.

scholarly journals Potential Assessment of Rumex spp. as a Source of Bioactive Compounds and Biological Activity

2021 ◽  
Vol 12 (2) ◽  
pp. 1824-1834
Keyword(s):  
Antioxidant Activity ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Gram Negative Bacteria ◽  
Meoh Extract ◽  
Gram Positive ◽  
Filter Paper Disc ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Antibacterial Performance

Secondary metabolites from the shoots and roots of three Rumex species collected from three different habitats were investigated (Rumex dentatus collected from cultivated land, R. pictus collected from the coastal desert and R. vesicarius collected from the inland desert) and tested for antioxidant activity as well as for anti-microbial activity against some human pathogenic bacteria. The present study indicated that the quantitative analysis of shoot and root extracts of three Rumex spp. were found to be rich in tannins and phenolics composition. The aerial parts of the three plants exhibited the highest significant values compared to the root parts. The MeOH extracts of Rumex species showed adequate antioxidant activity, wherein the IC50 values of the MeOH from the cultivated sample was 41.61 and 31.31 mg mL-1, coastal samples were 34.99 and 23.99 mg mL-1, while the sample of inland showed IC50 value of 41.59 and 31.67 mg mL-1, for root and shoot, respectively. Furthermore, using a filter paper disc assay, the MeOH extracts of the three Rumex species showed a substantial anti-microbial inhibitory effect on the growth of 10 pathogenic bacteria. According to sensitivity, the tested organisms could be sequenced as following: E. coli < K. pneumoniae ˂ S. typhi < P. aeruginosa for Gram-negative bacteria and B. subtilis < S. pneumoniae ˂ L. monocytoyenes < S. epidermis < S. aureus < B. cereus for Gram-positive bacteria. In addition, the antibacterial performance of R. dentatus root and R. vesicarius shoot MeOH extract is 100% broad spectrum against Gram-negative bacteria. A shoot of R. dentatus and R. pictus MeOH extract against Gram-positive bacteria is 83.3% broad spectrum. A further study is recommended for more characterization of the major compounds and assesses their efficiency and biosafety.

scholarly journals Synthesis, antioxidant and antimicrobial activity of carbohydrazones

2017 ◽  
Vol 82 (5) ◽  
pp. 495-508 ◽  
Author(s):  
Aleksandra Bozic ◽  
Nenad Filipovic ◽  
Irena Novakovic ◽  
Snezana Bjelogrlic ◽  
Jasmina Nikolic ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Dimethyl Sulphoxide ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Antioxidant Compound ◽  
Antioxidant And Antimicrobial Activity ◽  
Bacteria And Fungi

Fourteen mono- and bis-carbohydrazone ligands have been synthesized and characterized. Antioxidant activity of the substances was investigated together with possible (E)/(Z) isomerization, and explained on the most active antioxidant compound 4 in various dimethyl sulphoxide?water mixtures. The addition of water to the system was involved in the formation of hydrated molecules which was confirmed in NMR after the addition of D2O. The ligands were tested in vitro against Gram-positive and Gram-negative bacteria and fungi, and their activity was discussed in relation to the structure of investigated carbohydrazone.

scholarly journals Bacillus phage endolysin, lys46, bactericidal properties against Gram-negative bacteria

2020 ◽  
Author(s):  
Mohammad Reza Sarjoughian ◽  
Fereshte Rahmani ◽  
Shamsozoha Abolmaali ◽  
Shakiba Darvish Alipour Astaneh
Keyword(s):  
Bacillus Subtilis ◽  
Screening Program ◽  
Inhibitory Effect ◽  
Indicator Bacteria ◽  
Subtilis Strain ◽  
Gram Negative Bacteria ◽  
Gene Encoding ◽  
Gram Negative ◽  
Antibacterial Screening ◽  
Bacillus Phage

Background and Objectives: The great potential of bacteriophage for removing pathogen bacteria via targeting the cell wall is highly concerned. With a priority for overcoming drug-resistance, we screened against endolysins targeting Gram-negative bacteria to introduce a new antibacterial agent. This study was aimed to identify endolysins from the lysogenic phage of the Siphoviridea family in Bacillus subtilis. Materials and Methods: The Bacillus subtilis strain DDBCC46 was isolated from a preliminary antibacterial screening program. The endolysin (s) was extracted, concentrated with ammonium sulfate saturation, and their activity evaluated against the indicator bacteria. The phage particles were extracted from the bacteria using the minimum inhibition concentration of mitomycin C, followed by testing the phage inhibitory effect on the growth of indicator bacteria. The NCBI, Virus-Host DB, and EXPASY databases were used to obtain and confirm the sequences of the genes encoding PG hydrolases in Siphoviridea phages hosted in B. subtilis. Results: An 816 bp gene encoding an endolysin enzyme, was approved in the B. subtilis DDBCC 46, with specific primers of Bacillus phage SPP1. The purified-endolysin indicated antibacterial activity against Klebsiella pneumoniae, Salmonella typhimurium, Proteus (sp), and Escherichia coli. SDS-PAGE profiling followed by silica gel purification, led to introduceLys4630 as a therapeutic product and food preservative. Conclusion: lys4630 showed antibacterial effects on the common Gram-negative pathogens in clinics and food industries; E. coli, P. aeruginosa and Salmonella (sp).

scholarly journals Antibacterial Screening of some Heterocyclic Compounds Containing Quinoline Nucleus

2017 ◽  
Vol 76 ◽  
pp. 27-40
Author(s):  
Shipra Baluja ◽  
Nilesh Godvani ◽  
Sumitra Chanda
Keyword(s):  
Heterocyclic Compounds ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Spectroscopic Methods ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
H Nmr ◽  
Antibacterial Screening ◽  
Derivatives Of ◽  
Agar Well Diffusion Method

In this work, some novel derivatives of Cyanopyridines and Isoxazoles were synthesized using Vilsmeier-Haack reagent and their structures were confirmed by FTIR, 1H NMR and mass spectroscopic methods. The antibacterial activities of these synthesized compounds were studied in DMSO and DMF by agar well diffusion method against some Gram positive and Gram negative bacteria. It is observed that activity depends upon three S: solvent, strain and structure.

scholarly journals Volatile Constituents and Antibacterial Screening of the Essential Oil of Chenopodium Ambrosioides L. Growing in Nigeria

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Moses S. Owolabi ◽  
Labunmi Lajide ◽  
Matthew O. Oladimeji ◽  
William N. Setzer ◽  
Maria C. Palazzo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Cluster Analysis ◽  
Essential Oil ◽  
Chenopodium Ambrosioides ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Antibacterial Screening ◽  
Aerial Parts

The essential oil of the aerial parts of Chenopodium ambrosioides L. has been isolated by hydrodistillation and analyzed using GCMS. The major components were found to be α-terpinene (63.1%), p-cymene (26.4%) and ascaridole (3.9%). The oil displayed no antibacterial activity against either Gram-positive bacteria Bacillus cereus or Staphylococcus aureus, or the Gram-negative bacterium Escherichia coli (MIC = 1250 μg/mL). A cluster analysis of C. ambrosioides essential oils reveals at least seven distinct chemotypes: ascaridole, α-terpinene, α-pinene, p-cymene, carvacrol, α-terpinyl acetate, and limonene.

Sign in / Sign up

Export Citation Format

Share Document