scholarly journals Possible application of brewer’s spent grain in biotechnology

2013 ◽  
Vol 67 (2) ◽  
pp. 277-291 ◽  
Author(s):  
Jelena Pejin ◽  
Milos Radosavljevic ◽  
Olgica Grujic ◽  
Ljiljana Mojovic ◽  
Suncica Kocic-Tanackov ◽  
...  

Brewer?s spent grain is the major by-product in beer production. It is produced in large quantities (20 kg per 100 liters of produced beer) throughout the year at a low cost or no cost, and due to its high protein and carbohydrates content it can be used as a raw material in biotechnology. Biotechnological processes based on renewable agro-industrial by-products have ecological (zero CO2 emission, eco-friendly by-products) and economical (cheap raw materials and reduction of storage costs) advantages. The use of brewer?s spent grain is still limited, being basically used as animal feed. Researchers are trying to improve the application of brewer?s spent grain by finding alternative uses apart from the current general use as an animal feed. Its possible applications are in human nutrition, as a raw material in biotechnology, energy production, charcoal production, paper manufacture, as a brick component, and adsorbent. In biotechnology brewer?s spent grain could be used as a substrate for cultivation of microorganisms and enzyme production, additive of yeast carrier in beer fermentation, raw material in production of lactic acid, bioethanol, biogas, phenolic acids, xylitol, and pullulan. Some possible applications for brewer?s spent grain are described in this article including pre-treatment conditions (different procedures for polysaccharides, hemicelluloses, and cellulose hydrolysis), working microorganisms, fermentation parameters and obtained yields. The chemical composition of brewer?s spent grain varies according to barley variety, harvesting time, malting and mashing conditions, and a quality and type of unmalted raw material used in beer production. Brewer?s spent grain is lignocellulosic material rich in protein and fibre, which account for approximately 20 and 70% of its composition, respectively.

2000 ◽  
Vol 15 (1) ◽  
pp. 2-8 ◽  
Author(s):  
N.C. Wagner ◽  
S. Ramaswamy ◽  
U. Tschirner

AbstractA pre-economic feasibility study was undertaken to determine the potential of cereal straw for industrial utilization in Minnesota. Specifically, utilizing straw for pulp and paper manufacture was of interest. The availability of cereal straw fiber supplies at various locations across the state of Minnesota, along with pre-processing issues such as transportation, harvesting, handling, and storage, are discussed and priced. The greatest economic advantage of straw for industrial use appears to be the low cost of the raw material compared to traditional raw materials. This also provides an excellent opportunity for additional income for farmers. The methodology and information provided here should be helpful in evaluating the feasibility of utilizing straw for other industrial purposes in other parts of the world. However, in some Third World countries, long-standing on-farm, traditional uses of cereal straws for fuel, fiber, and animal feed may limit their availability for industrial utilization.


2021 ◽  
Author(s):  
Magdalena Lech

Abstract The brewery spent grain (BSG) is a lignocellulosic waste material produced in a huge amount around the world. Strict environmental protection law requires proper utilization. BSG can be transformed into easy-fermentable carbohydrates as a result of hydrolysis. This may be a low-cost raw material for biotechnological fermentation. The literature provides a lot of information that hydrolysis of lignocellulose creates by-products which can be potentially noxious to bacteria cells employed in fermentation. This research examined the influence of most of these by-products: furfural, acetic, formic, gallic, and levuilnic acid, on the LA fermentation effectiveness. These components were introduced to Lactobacillus cultures in various concentrations. The rate of cell growth, glucose consumption, and lactic acid production were measured. This components affects in various extent on LA formation in the culture. In any case, there is a critical value of them harms the fermentation, due to the reduced ability of bacteria propagation. Lower concentrations of bio-catalyst lead to an LA production efficiency drop. The LA concentrations in flasks after one day of propagation with the 2.1 [g/L] of appropriate inhibitor drop app. [%] 16 (F), 22 (GA), 8 (LevA), 40 (AA), and 100 (FA) in comparison to the flask without any inhibitor.


2020 ◽  
Vol 9 (11) ◽  
pp. e45491110225 ◽  
Author(s):  
Anelise Christ-Ribeiro ◽  
Janaína Barreto Alves ◽  
Leonor Almeida de Souza-Soares ◽  
Eliana Badiale-Furlong

The idea that associated agro-industrial by-products only with the production of animal feed has been gradually being replaced, as the scientific community is interested in these products, as low-cost nutrients for the composition of food and/or culture media in research biotechnological to increase or improve nutrients or characteristics inherent in the raw material. Thus, this work aims to characterize input for baking with claim of natural fortification from the fermentation by Saccharomyces cerevisiae. For this, biomass was characterized in terms of its composition (ashes, fibers, lipids, proteins, and carbohydrates) and antioxidant activity of phenolic compounds. The results show an increase in nutrients such as ash, proteins and fibers, in addition the free and bound phenolic compounds present in the biomass showed antioxidant potential. Therefore, fermented rice bran is a potential input to be used in baking.


Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 515
Author(s):  
Lady Laura Del Rio Osorio ◽  
Edwin Flórez-López ◽  
Carlos David Grande-Tovar

The food sector includes several large industries such as canned food, pasta, flour, frozen products, and beverages. Those industries transform agricultural raw materials into added-value products. The fruit and vegetable industry is the largest and fastest-growing segment of the world agricultural production market, which commercialize various products such as juices, jams, and dehydrated products, followed by the cereal industry products such as chocolate, beer, and vegetable oils are produced. Similarly, the root and tuber industry produces flours and starches essential for the daily diet due to their high carbohydrate content. However, the processing of these foods generates a large amount of waste several times improperly disposed of in landfills. Due to the increase in the world’s population, the indiscriminate use of natural resources generates waste and food supply limitations due to the scarcity of resources, increasing hunger worldwide. The circular economy offers various tools for raising awareness for the recovery of waste, one of the best alternatives to mitigate the excessive consumption of raw materials and reduce waste. The loss and waste of food as a raw material offers bioactive compounds, enzymes, and nutrients that add value to the food cosmetic and pharmaceutical industries. This paper systematically reviewed literature with different food loss and waste by-products as animal feed, cosmetic, and pharmaceutical products that strongly contribute to the paradigm shift to a circular economy. Additionally, this review compiles studies related to the integral recovery of by-products from the processing of fruits, vegetables, tubers, cereals, and legumes from the food industry, with the potential in SARS-CoV-2 disease and bacterial diseases treatment.


Energies ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4443
Author(s):  
Rossana Liguori ◽  
Anna Pennacchio ◽  
Luciana Porto de Souza Vandenberghe ◽  
Addolorata De Chiaro ◽  
Leila Birolo ◽  
...  

Brewer’s spent grain (BSG), the solid residue of beer production, is attracting significant attention as raw material for the production of added value substances, since until recently it was mainly used as animal feed or deposited in landfills, causing serious environmental problems. Therefore, this work aimed at developing a bioprocess using BSG as a substrate for the production of cellulases and xylanases for waste saccharification and bioenergy production. Different fungi were analyzed for their cellulolytic and xylanolytic abilities, through a first screening on solid media by assessment of fungal growth and enzyme production on agar containing carboxylmethylcellulose or xylan as the sole carbon source, respectively. The best cellulase and xylanase producers were subjected to quantitative evaluation of enzyme production in liquid cultures. Aspergillus niger LPB-334 was selected for its ability to produce cellulase and xylanase at high levels and it was cultivated on BSG by solid state fermentation. The cellulase production reached a maximum of 118.04 ± 8.4 U/g of dry substrate after 10 days of fermentation, while a maximum xylanase production of 1315.15 ± 37.5 U/g of dry substrate was reached after 4 days. Preliminary characterization of cellulase and xylanase activities and identification of the enzymes responsible were carried out.


Author(s):  
Endah Retno Dyartanti ◽  
Margono Margono ◽  
Anisa Raditya Nurohmah ◽  
Shofirul Sholikhatun Nisa ◽  
Novan Riantosa

Ethanol as a renewable fuel has been widely produced in various countries. One source of raw material for producing ethanol is starch. The process of producing ethanol from starch needs to be pretreated so that starch molecules can split into smaller ones. However, this process requires pre-treatment which will expensive more than ethanol from sugar. There are two types of pretreatment i.e. two-step ethanol production and direct fermentation. There is two kind of hydrolysis, acid hydrolysis, and enzymatic hydrolysis. Two-step ethanol production is a conventional method that separates pretreatment and fermentation process, while direct fermentation is the direct production of starch into ethanol using recombinant yeast that co-produces enzymes such as amylose and glucoamylase. Two-step ethanol production has the advantage of high yield but needs high cost whereas, direct fermentation has the advantage of low-cost production but needs longer time. Common starch to ethanol production consists of two stages, namely hydrolysis of raw materials into glucose and fermentation into ethanol. Both of these processes can be run on average at temperatures of 30-80<sup>o</sup>C with a pH range of 4-6 and varying time intervals. The enzyme used depends on the source of the starch, but the most commonly used is <em>Saccharomyces cerevisiae</em>.


2010 ◽  
Vol 64 (5-6) ◽  
pp. 421-428
Author(s):  
Djordje Okanovic ◽  
Dragan Milicevic ◽  
Milutin Ristic

A significant group of inedible by-products from industrial slaughterhouses that can be used as a raw material for processing into quality feed for pigs and pets are byproducts of slaughtered poultry. The poultry industry yields a large amount of inedible byproducts of which the removal or processing requires hygienic and economic solutions. These activities primarily include the protection of the environmental from degradation processes and biological pollution, as well as securing high-protein animal feed. The incorporation of inedible slaughtered poultry by-products into food for animals involves the application of various technological processes. These processes should be simple and feasible for investments where it is necessary to obtain a quality product. At the same time, it must be taken into account that the production process does not create new secondary products or waste gases that could contaminate the environment. Scientific knowledge in the field of exploitation of inedible slaughtered poultry by-products provides the necessary basis for calculating the amount of certain types of raw materials.


2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Prabin Koirala ◽  
Ndegwa Henry Maina ◽  
Hanna Nihtilä ◽  
Kati Katina ◽  
Rossana Coda

Abstract Background Lactic acid bacteria can synthesize dextran and oligosaccharides with different functionality, depending on the strain and fermentation conditions. As natural structure-forming agent, dextran has proven useful as food additive, improving the properties of several raw materials with poor technological quality, such as cereal by-products, fiber-and protein-rich matrices, enabling their use in food applications. In this study, we assessed dextran biosynthesis in situ during fermentation of brewers´ spent grain (BSG), the main by-product of beer brewing industry, with Leuconostoc pseudomesenteroides DSM20193 and Weissella confusa A16. The starters performance and the primary metabolites formed during 24 h of fermentation with and without 4% sucrose (w/w) were followed. Results The starters showed similar growth and acidification kinetics, but different sugar utilization, especially in presence of sucrose. Viscosity increase in fermented BSG containing sucrose occurred first after 10 h, and it kept increasing until 24 h concomitantly with dextran formation. Dextran content after 24 h was approximately 1% on the total weight of the BSG. Oligosaccharides with different degree of polymerization were formed together with dextran from 10 to 24 h. Three dextransucrase genes were identified in L. pseudomesenteroides DSM20193, one of which was significantly upregulated and remained active throughout the fermentation time. One dextransucrase gene was identified in W. confusa A16 also showing a typical induction profile, with highest upregulation at 10 h. Conclusions Selected lactic acid bacteria starters produced significant amount of dextran in brewers’ spent grain while forming oligosaccharides with different degree of polymerization. Putative dextransucrase genes identified in the starters showed a typical induction profile. Formation of dextran and oligosaccharides in BSG during lactic acid bacteria fermentation can be tailored to achieve specific technological properties of this raw material, contributing to its reintegration into the food chain.


Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 681
Author(s):  
Monika Sterczyńska ◽  
Marek Zdaniewicz ◽  
Katarzyna Wolny-Koładka

During the production of beer, and especially beer wort, the main wastes are spent grain and hot trub, i.e., the so-called “hot break.” Combined with yeast after fermentation, they represent the most valuable wastes. Hot trub is also one of the most valuable by-products. Studies on the chemical composition of these sediments and their rheological properties as waste products will contribute to their effective disposal and even further use as valuable pharmaceutical and cosmetic raw materials. So far, hot trub has been studied for morphology and particle distribution depending on the raw material composition and beer wort extract. However, there are no preliminary studies on the rheological properties of hot trub and hops. In particular, no attention has yet been paid to the dependence of these properties on the hop variety or different protein sources used. The aim of this study was to examine the effect of different hopping methods on hot trub viscosity and beer wort physicochemical parameters. Additionally, the hop solutions were measured at different temperatures. A microbiological analysis of hop sediments was also performed to determine the post-process survival of selected microorganisms in these wastes. For manufacturers of pumps used in the brewing industry, the most convenient material is that of the lowest viscosity. Low viscosity hot trub can be removed at lower velocities, which reduces costs and simplifies washing and transport. The sediments also had similar equilibrium viscosity values at high shear rates.


Author(s):  
P.K. Dang ◽  
N.T.P. Giang ◽  
T.T. Nguyen ◽  
S. Chu-Ky ◽  
N.C. Oanh ◽  
...  

Background: In Vietnam, animal feed mainly depends on imported raw materials, while available agro-industrial by-products are often released into the environment and only a its unsubstantial portion used as an animal feed. Therefore, efficient use of these by-products as animal feed is essential. This work aimed to evaluate the effect of dietary levels of rice distiller’s dried grain (Rice DDG) on broiler performance, meat yield and meat quality. Methods: The research was conducted at the experimental station, Vietnam National University of Agriculture, Vietnam, from May to September 2020. A total of 240 a day-old broiler chicks were randomly assigned into 4 groups with 3 replications to receive 4 diets with different levels of RDDG at 0, 5, 8 and 10% for 14 weeks. Growth, meat yield and meat quality were estimated. Result: ADG was higher in broiler chickens fed diets with rice DDG compared to that in control diet at 12 and 14 weeks (P less than 0.05) while ADFI and FCR were not affected by the diets. There were significantly no differences in meat yield and quality among the diets. In conclusion, rice DDG could be used to partially replace main ingredients in chicken diets.


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