scholarly journals STUDY OF PREPARATION METHOD IN ANTIOXIDANTS ANALYSIS FROM KELOR SEEDS (Moringa oleifera L.) TOFU COAGULATION

2017 ◽  
Vol 1 (2) ◽  
pp. 73
Author(s):  
Selly Harnesa Putri ◽  
Farah Nuranjani ◽  
Irfan Ardiansah
Keyword(s):  
Free Radical ◽  
Extraction Process ◽  
High Yield ◽  
Solvent Ratio ◽  
Natural Coagulant ◽  
Assisted Extraction ◽  
Dpph Method ◽  
Ultrasonic Assisted ◽  
The Impact ◽  
Yield And Purity

Currently, Indonesia is exporter of the moringa seed. Moringa seed is a plant which is contain a vegetable oil with many benefits, especially for health. In addition to the utilization of moringa seeds used as vegetable oils, the seeds of this moringa as a natural coagulant, supported by its antioxidant is suitable combined in its use as a natural coagulant on tofu. The analytical method used in the antioxidant content testing using DPPH method is an easy, fast and sensitive method using DPPH free radical (1,1-diphenyl-2-picrilhidrazil). In antioxidant analysis required materials of extract, which high yield and purity consisting of extraction method of multilevel maceration extraction with 3 solvents (n-hexane, 70% of acetone and 96% of ethanol) 1: 3 solvent and solvent ratio and Ultrasonic Assisted Extraction (UAE). The result of the research showed that the extraction process using multilevel maceration extraction with high yield and purity. Determination of extract concentration for IC50 calculation measured by using a spectrophotometer so that the percentage of inhibition is 722,01 which concentration needed to 50 % can inhibit free radical in antioxidants analysis. The impact of research results is utilized optimally on extract moringa seeds with many benefits especially for health.   

scholarly journals Optimization and Isolation of 4,8,12,16-Tetramethylheptadecan-4-olide from Deinbollia pinnata

2019 ◽  
Vol 31 (11) ◽  
pp. 2503-2511
Author(s):  
Y. Rufai ◽  
N. Basar ◽  
A. Sani
Keyword(s):  
2D Nmr ◽  
Bulk Sample ◽  
Extraction Process ◽  
High Yield ◽  
Solvent Ratio ◽  
Isolation And Purification ◽  
Assisted Extraction ◽  
Sample Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Manual agitation on ultrasonic assisted extraction process, fractionation, isolation and purification afforded 4,8,12,16-tetramethylheptadecan-4-olide (1) along with 11 compounds 2-12 i.e., squalene (2), phytyl palmitate (3), lupeol (4), taraxasterol (5), myristic acid (6), palmitic acid (7), campesterol (8), stigmasterol (9), λ-sitosterol (10), stigmastan-3, 5-diene (11) and stigmasta-5,22-diene-3-ol acetate (12). Their structures were elucidated spectroscopically (2D NMR, IR, GC-MS and 1D NMR). The optimal conditions for high yield of extracts were obtained at 45 °C, after 35 min and solvent ratio 50:50 mL for 83.01 % yield; which was applied on agita-sonication process for bulk sample extraction of D. pinnata leaves with single solvent at a time. Thus, this work provides alternative method to overcome large sample extraction for phyto-constituents of isolation of 4,8,12,16-tetramethylheptadecan-4-olide (1) with ten other compounds from this specie pinnata and genius Deinbollia except stigmasterol (9).

scholarly journals Pengaruh Amplitudo Ultrasonik dan Waktu Ekstraksi Terhadap Rendemen dan Mutu Oleoresin Pala

2021 ◽  
Vol 8 (2) ◽  
pp. 45-52
Author(s):  
I Wayan Budiastra ◽  
Sutrisno Suro Mardjan ◽  
Ahmuhardi Abdul Azis
Keyword(s):  
Extraction Process ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Yield And Quality ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Long Time ◽  
Four Levels ◽  
Derivative Products

Nutmeg oleoresin is one of the nutmeg derivative products with high values produced from the extraction process. The conventional extraction process using maceration takes a long time so that it is less suitable for industries needs. This study aims to examine the UAE direct sonication method for increasing yield and quality of nutmeg eleoresin. Nutmeg with optimal maturity level were harvested, dried and milled into 60 mesh particle size. Nutmeg powder of 200 g was placed in beaker glass filled with etanol 800 ml (material and solvent ratio 1:4) and UAE was carried out using a sonicator with a frequency of 20 kHz and power of 700 W. Four ultrasound amplitude levels (45, 60, 75, 90%) and four levels of extraction time (30, 45, 60, 75 minutes) were taken as UAE treatments. Extraction using maceration at room temperature for 7 hours was done as control.  The results showed that the greater the amplitude of the ultrasound, the higher the yield of nutmeg oleoresin. The longer the extraction time, the higher the yield of nutmeg oleoresin. Ultrasonic assisted extraction can increase nutmeg oleoresin yield by 11 to 52% and faster time (<= 1,25 hour) than maceration extraction method (7 hours).

scholarly journals PENENTUAN KANDUNGAN ANTIOKSIDAN DALAM RIMPANG KENCUR YANG DIEKSTRAKSI DENGAN BANTUAN GELOMBANG ULTRASONIK

2018 ◽  
Vol 3 (2) ◽  
pp. 46-56
Author(s):  
Refilda Suhaili
Keyword(s):  
Method Validation ◽  
Extraction Process ◽  
Kaempferia Galanga ◽  
Antioxidant Content ◽  
Assisted Extraction ◽  
Percent Recovery ◽  
Dpph Method ◽  
Total Antioxidant ◽  
Ultrasonic Assisted ◽  
Long Time

Kaempferia galanga L. rhizome is used a traditional medicine and as seasoning. Kaempferia galanga L. rhizome has many bioactivies beneficial to antioxidant. Extraction of antioxidant by maceration method takes a long time and the extraction process is not perfect so ultrasonic assisted extraction (UAE) are used to accelerate the extraction process. The propose of the research is to extract antioxidant from Kaempferia galanga L. rhizome by UAE and to determinate the antioxidant content by 1,1-diphenyl-2-picrilhydrazyl (DPPH) method. The optimum condition for extraction were 20 mL methanol as the solvent, the weight of sample 1,5 g, and extraction time 60 minutes. The total antioxidant content from Kaempferia galanga L. rhizome extraction by maceration and UAE were 0.6688 ± 0.02 and 0.8126 ± 0.02 mg/g DW. This study also validated the method of determining the total antioxidant content extracted with ultrasonic. The results of the method validation obtained linearity (r) = -0.9984, LoD value = 0.0283 mg/L, LoQ value = 0.0947 mg/L, precision (SDR = 1.97% and HORRAT = 0.96) and percent recovery 103.21%. Based on the method validation value, it can be said that the determination of the total antioxidant content in the kencur rhizome extracted with the help of UAE can be applied.

Kinetics on the Ultrasonic-Assisted Extraction of Polysaccharides from Limonium bicolor kunze (Bge.)

2014 ◽  
Vol 1003 ◽  
pp. 3-6
Author(s):  
Xiao Song ◽  
Peng Zhao ◽  
Qing Hua Meng ◽  
Zhi Shu Tang ◽  
Chang Li Wang
Keyword(s):  
Kinetic Equation ◽  
Extraction Process ◽  
Ultrasonic Extraction ◽  
Second Law ◽  
Technical Design ◽  
Kinetics Parameters ◽  
Limonium Bicolor ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

To explore the kinetics parameters in the ultrasonic-assisted extraction from Limonium bicolor Kunze (Bge.) , the kinetic equation for polysaccharide ultrasonic extraction process was established with ball model. According to Fick’s second law of diffusion, extraction process was analysed. The results can provide the valuable theory basis for the technical design and further research of polysaccharide extraction process.

scholarly journals Chemical composition of ultrasonic-assisted n-hexane extracts of Sideritis scardica Grieseb. and Sideritis raeseri Boiss. & Heldr. (Lamiaceae) from Macedonia and Albania

2011 ◽  
Vol 56 ◽  
pp. 45-56 ◽  
Author(s):  
Bujar Qazimi ◽  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Svetlana Kulevanova
Keyword(s):  
Fatty Acids ◽  
Chemical Composition ◽  
Plant Material ◽  
Extraction Process ◽  
Triterpene Alcohols ◽  
Assisted Extraction ◽  
Aromatic Alcohols ◽  
Ultrasonic Assisted ◽  
Hexane Extracts ◽  
Ultrasonic Assisted Extraction

Chemical composition of n-hexane extracts obtained from dried over-ground parts of two species of Sideritis, S. scardica Grieseb. and S. raeseri Boiss. & Heldr. (Lamiaceae) was analyzed using GC/FID/MS. The collection of plants was made on different locations in the western part of Macedonia and the southern part of Albania, comprising twelve different samples of plant material. The ultrasonic-assisted extraction process was used for preparation of the n-hexane extracts yielded 0.73-3.33 % and 9.11-10.44 % of extracts for S. scardica and S. raeseri, respectively. Over one hundred constituents of the extracts were identified, belonging to several classes of components: diterpenes, hydrocarbons, dominantly present in each of the extracts, followed by fatty acids, aliphatic and aromatic alcohols, sterols, triterpene alcohols, and monoterpenes and sesquiterpenes, which were found in much smaller amounts or only in traces. The most abundant constituents of the extracts of both species of Sideritis were two diterpene components, both with M=286, which were not fully identified. Large percentages of nonacosane (1.71-12.22% and 7.46-19.68% for S. scardica and S. raeseri, respectively) and hentriacontane (4.48-20.79% and 8.09-30.31 % for S. scardica and S. raeseri, respectively) were also found in the extracts of both species.

scholarly journals Antioxidant Activity of Extract from Ultrasonic-Assisted Extraction of Durian Peels

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Bambang Kunarto ◽  
Ely Yuniarti Sani
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Extraction Process ◽  
Extraction Time ◽  
Total Flavonoids ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Total Flavonoids Content ◽  
Phenolics Content ◽  
Ultrasonic Assisted Extraction

The increase in durian production results in the accumulation of durian peel waste. The bioactive component of durian peel has the potential to be used as an antioxidant. Thus, there is a need to carry out an extraction process to obtain  bioactive compounds from durian peel. However, conventional extraction methods cause damage to phenolic compounds due to oxidation, hydrolysis and ionization reactions during the extraction process. Therefore, durian peel extraction was carried out using ultrasonic assisted extraction method (UAE) in this study. The purpose of this study was to investigate the effect of varying ratios of durian peel to ethanol solvents and extraction time on the yield, total phenolics content, total flavonoids content and antioxidant activity. The results of the research data were analyzed using a two-factor completely randomized design, which included variations of the ratio of durian peel to ethanol solvents and extraction time The Duncan’s New Multiple Range Test (DNMRT) was carried out as a follow up test to determine the differences in each treatment at a significance level of 0.05. The results showed that the best treatment for peel extraction using ultrasonic assisted extraction was a 1: 9 ratio of durian peel to ethanol at an extraction time of 20 minutes. The extraction of durian peel under this condition gave the highest yield of 12.77 ± 0.16%, antioxidant activity (IC50) of 38.33 ± 0.12 ppm, total phenolic content of 63.30 ± 0.08 mgGAE / g and total flavonoids content of 47.53 ± 0.48 mgQE / g. In addition, total phenolics content and total flavonoid content showed a strong correlation to the antioxidant activity of durian peel extract.

scholarly journals Optimization of Anthocyanin Extraction from Cockspur Coral (Erythrina Crista-Galli L.) Petals with Microwave-Assisted Extraction (MAE) using Response Surface Methodology

2021 ◽  
Vol 21 (2) ◽  
pp. 143
Author(s):  
Astrilia Damayanti ◽  
Bayu Triwibowo ◽  
Megawati Megawati ◽  
Miftahuddin Azhari ◽  
Sandra Anggita Fadriana
Keyword(s):  
Response Surface ◽  
Extraction Process ◽  
Operating Conditions ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Surface Method ◽  
Assisted Extraction ◽  
Order Polynomial ◽  
Independent Variable

Cockspur coral (Erythrina crista-galli L) petals are flowers that contain anthocyanins and active compounds of flavonoids and tannins. This study aims to determine the optimum conditions for the anthocyanin extraction process of cockspur coral petals using Microwave-Assisted Extraction (MAE), analyzed through the Response Surface Method (RSM). This process uses ethanol-hydrochloric acid solvents and a Box-Behnken experimental design involving three factors, namely the solvent ratios (w/v) (1:5, 1:15, and 1:25), microwave powers (300, 450, and 600 watts), and extraction times (3, 9, and 15 minutes). As a result, the second-order polynomial model was enhanced and sufficient to explain the variation of the data that denoted the significant correlation with the independent variables and the response. Derringer's desired function methodology was used for optimizing studies and generated ideal conditions for each or combined an independent variable.  The optimum anthocyanin extract of 5.82 mg/L was obtained at a power condition of 325,5 Watts, an extraction time of 3.05 minutes, and a solvent ratio of 20.5. Meanwhile, the operating conditions at a power of 310.8 Watts, a time of 14.94 minutes, and a solvent ratio of 24.96 resulted in the optimum color intensity (IC) of 1040.26. In the meantime, the optimum antioxidant activity was obtained at a power of 585.97 Watts, a time of 4.93 minutes, and a solvent ratio of 5.43 with IC50 of 0.115.

scholarly journals Optimization of Extraction Conditions of Bioactive Compounds by Ultrasonic-Assisted Extraction from Artichoke Wastes

2021 ◽  
Vol 68 (3) ◽  
pp. 658-666
Author(s):  
Izzet Turker ◽  
Hilal Isleroglu
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Extraction Process ◽  
Extraction Yield ◽  
Extraction Time ◽  
Total Phenolic ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Extraction Processes ◽  
Ultrasonic Assisted Extraction

In this study, bioactive compounds were extracted by ultrasonic-assisted extraction and classical extraction processes using distilled water as solvent from artichoke leaves which are considered as agricultural wastes. Antioxidant capacity, total phenolic and total flavonoid content values of the obtained bioactive extracts were determined, and extraction yields and times were evaluated to compare the extraction processes. Also, the optimum extraction conditions of ultrasonic-assisted extraction (extraction time and ultrasonic power) which provide the highest extraction yield were determined using D-optimal design by ‘desirability’ function approach. According to the results, bioactive extracts having high antioxidant capacity were obtained at shorter times and higher extraction yields were achieved by ultrasonic-assisted extraction process than classical extraction. The highest extraction yield was estimated as 98.46% with an application of 20.05 minutes of extraction time and 65.02% of ultrasonic amplitude for the ultrasonic-assisted extraction process.

scholarly journals Study on the Extraction of Polyphenols from Blueberry Leaves and their antioxidant properties

2021 ◽  
Vol 251 ◽  
pp. 02059
Author(s):  
Fenghong Liu ◽  
Xianhao Cheng ◽  
Wenguang Liu ◽  
Jing Miu ◽  
Jiahui Wang ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Orthogonal Experiment ◽  
Antioxidant Properties ◽  
High Yield ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Solid Liquid ◽  
Blueberry Leaves

Blueberry leaf as materials, ethanol ultrasonic assisted extraction of blueberry polyphenols, ethanol concentration and solid-liquid ratio on blueberry ultrasonic time leaves influence the yield of polyphenols were researched, and the orthogonal experiment to optimize the ethanol ultrasonic assisted extraction technological conditions of blueberry leaf polyphenol polyphenols was used. The results showed that the optimal extraction conditions of polyphenols from blueberry leaves were as follows, 9.06% with 4min ultrasonic time, 80% ethanol concentration and 1:45 (g/mL) ratio. Ultrasonic assisted extraction is a convenient and high yield method, which is suitable for the extraction of polyphenols from blueberry leaves.

scholarly journals Optimasi Ekstraksi Berbantu Gelombang Ultrasonik pada Biji Melinjo Kerikil (Gnetum gnemon L., ‘Kerikil’) Menggunakan Response Surface Methodology

2019 ◽  
Vol 8 (3) ◽  
pp. 104
Author(s):  
Bambang Kunarto ◽  
Sutardi Sutardi ◽  
Supriyanto Supriyanto ◽  
Chairil Anwar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Reducing Power ◽  
Extraction Process ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Ultrasonic Bath ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Biji melinjo kerikil mengandung senyawa fenolik dan resveratrol sehingga berpotensi sebagai antioksidan, oleh karena itu perlu dilakukan ekstraksi. Metoda ekstraksi konvensional (maserasi, perebusan, refluxing) mempunyai kelemahan yaitu terjadinya kerusakan senyawa fenolik akibat reaksi oksidasi, hidrolisis dan ionisasi selama proses ekstraksi. Oleh karena itu, pada penelitian ini ekstraksi biji melinjo kerikil dilakukan menggunakan metode ekstraksi berbantu gelombang ultrasonik. Penelitian ini bertujuan untuk optimasi ekstraksi biji melinjo kerikil berbantu gelombang ultrasonik yang dilakukan pada berbagai waktu ekstraksi, suhu ekstraksi dan konsentrasi pelarut etanol. Surface response methodology digunakan untuk optimasi kondisi ekstraksi. Biji melinjo kerikil diekstrak menggunakan ultrasonic bath pada frekuensi 40 kHz dengan berbagai suhu (25, 30, 35, 40, 45oC), waktu ekstraksi (10, 20, 30, 40, 50 menit) dan konsentrasi pelarut etanol (40, 50, 60, 70 80%). Hasil penelitian menunjukkan bahwa kondisi optimum ekstraksi biji melinjo kerikil berbantu gelombang ultrasonik adalah pada suhu 30,18oC, waktu 33,01 menit dan konsentrasi pelarut etanol 71,04%. Pada kondisi ini, diperoleh yield ekstrak 18,41 ± 0,01%, total fenolik 11,26 ± 0,06 mg GAE/g, total flavonoid 533,70 ± 0,18 mg CE/100g, resveratrol 7,64 ± 0, 03%, IC50 sebesar 59,52 ± 0,04 ppm dan reducing power 76,31 ± 0,08%. Sebagai kesimpulan, optimasi ekstraksi biji melinjo kerikil berbantu gelombang ultrasonik menggunakan response surface methodology ini cukup baik karena nilai respon yang sebenarnya sesuai dengan nilai respon yang diprediksi.Melinjo kerikil seeds contain phenolic and resveratrol compounds so that it has the potential as an antioxidant, therefore extraction needs to be done. Conventional extraction methods (maceration, boiling, refluxing) have the disadvantage of devasting phenolic compounds due to oxidation, hydrolysis and ionization reactions during the extraction process. Therefore, in this study the extraction of melinjo kerikil seeds done by using the ultrasonic-assisted extraction method. The study aims to optimize ultrasonic-assisted extraction of melinjo kerikil seeds that be done at various extraction times, extraction temperatures and ethanol solvents concentrations. Response surface methodology was used to optimize experimental condition for extraction. Melinjo kerikil seeds were extracted by using ultrasonic bath at a frequency of 40 kHz with various temperatures (25, 30, 35, 40, 45oC), extraction time (10, 20, 30, 40, 50 minutes) and ethanol solvents concentrations (40, 50, 60, 70 80 %). The results showed that the optimum conditions for ultrasonic-assisted extraction of melinjo kerikil seeds were at a temperature of 30.18oC, a time of 33.01 minutes and an ethanol solvent concentration of 71.04%. In this condition, obtained the extract yield 18.41 ± 0.01%, total phenolic 11.26 ± 0.06 mg GAE/g, total flavonoids 533.70 ± 0.18 mg CE/100g, resveratrol 7.64 ± 0,03%, IC50 in the amount of 59.52 ± 0.04 ppm and reducing power 76.31 ± 0.08%. As a conclusion, the optimization ultrasonic-assisted extraction of melinjo kerikil seeds by using response surface methodology is quite good because the actual response value is in accordance with the predicted response value.

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