scholarly journals Relationship of arctic fox (Alopex lagopus L.) sperm morphology with age of males, sperm concentration, ejaculate volume and acrosin activity

2018 ◽  
Vol 18 (3) ◽  
pp. 741-752 ◽  
Author(s):  
Karolina Stasiak ◽  
Stanisław Kondracki ◽  
Maria Iwanina
Keyword(s):  
Sperm Morphology ◽  
Semen Quality ◽  
Morphological Changes ◽  
Sperm Concentration ◽  
Arctic Fox ◽  
Young Males ◽  
Arctic Foxes ◽  
Relationship Of ◽  
Acrosin Activity ◽  
Sperm Acrosome

Abstract The aim of the study was to determine the relationship of sperm morphology with age of males, ejaculate concentration and volume, as well as with acrosin activity determined in sperm acrosome extracts. The study used manually collected ejaculates from 9 male arctic foxes, including 6 young males aged one year and 3 older males (between 3 and 5 years of age). All of the 39 ejaculates used in the study were classified as normal based on motility exceeding 70%. The ejaculates collected from the foxes were evaluated for volume, sperm concentration and frequency of morphological changes including primary and secondary defects. The spermiograms of the male arctic foxes were classified according to a six-grade subjective scale. In addition, acrosin activity was determined in the sperm acrosome extracts. The data were analysed using the criteria of male age, sperm concentration, ejaculate volume, and acrosin activity. The morphology of arctic fox spermatozoa was dependent on the age of the male. A greater number of morphologically altered spermatozoa tended to occur in the ejaculates of young foxes, which were in their first breeding season. In addition, statistical analysis revealed positive relationships between the frequency of morphological changes in sperm and their ejaculate concentration. In contrast, there were no significant correlations between the percentages of morphologically changed spermatozoa and the ejaculate volume and the content of acrosin, which is an indicator of acrosomal integrity. Semen quality is dependent on the number of sperm in ejaculate with morphological defects which prevent oocyte fertilization. Therefore, morphological assessment of semen, which covers both the number and type of morphological changes, is highly useful when selecting appropriate males for reproduction.

Relationship between semen quality and seminal plasma cholesterol, triacylglycerols and proteins in the domestic cat

2019 ◽  
Vol 22 (10) ◽  
pp. 882-889 ◽  
Author(s):  
María F García ◽  
Romina Nuñez Favre ◽  
María C Stornelli ◽  
Ramiro Rearte ◽  
María C García Mitacek ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Count ◽  
Sodium Dodecyl ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Domestic Cat ◽  
Sds Page ◽  
The Relationship

Objectives The current study aimed to evaluate the relationship between specific seminal plasma components – cholesterol (CHOL), triacylglycerols (TAG) and total protein (PROT) concentrations – and semen quality in cats. A further aim was to determine the relationship between specific seminal protein bands and semen quality. Methods Thirteen toms, 2–5 years of age, were included. Semen collection was performed by electroejaculation every 4 weeks. Fifty-eight ejaculates were assessed for motility, velocity, volume, sperm concentration, total sperm count, viability, acrosome integrity, plasma membrane integrity and sperm morphology. Samples were divided into two groups: good semen quality (GSQ) and poor semen quality (PSQ). After evaluation, seminal plasma was separated from the sperm by centrifugation and stored at −20°C. CHOL, TAG and PROT concentrations were then assessed and seminal plasma protein profile was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results Seminal plasma CHOL and TAG concentrations, motility, velocity, sperm concentration, total sperm count and sperm morphology were significantly higher in GSQ cats compared with PSQ cats ( P <0.01). Moreover, seminal plasma SDS-PAGE analysis showed an identifiable extra band exclusively in the GSQ group. Conclusions and relevance Data obtained in this study showed that seminal plasma CHOL and TAG concentrations and specific protein bands could be used to improve semen evaluation in toms. In this sense, the 14 kDa protein band could be a valuable marker for semen quality in the cat and should be further investigated. However, more studies are necessary to determine its relationship with fertility.

Effect of age and breeding season on sperm acrosin activity in the arctic fox (Alopex lagopus L.)

2014 ◽  
Vol 17 (1) ◽  
pp. 177-179 ◽  
Author(s):  
K. Stasiak ◽  
B. Janicki
Keyword(s):  
Breeding Season ◽  
The Arctic ◽  
Arctic Fox ◽  
Alopex Lagopus ◽  
A Value ◽  
Arctic Foxes ◽  
The Mean ◽  
One Year ◽  
Acrosin Activity ◽  
Effect Of Age

Abstract The objective of this study was to determine the effect of age and reproductive season on selected properties of semen from the arctic fox, Alopex lagopus L. The experiment used 40 ejaculates collected manually from 6 animals (3 foxes aged one year and 3 foxes older than three years). Statistically less semen (0.39 cm3) was collected from the young compared to the older animals, and the ejaculates obtained were characterized by higher concentration of spermatozoa (195.04 x 106/cm3). In turn, sperm acrosomal extracts from the older animals contained statistically more acrosin (6,4 mU/106 spermatozoa). In the sperm acrosomal extracts prepared during the first semen sampling, the mean acrosin activity did not exceed 2.3 mU/million spermatozoa. At subsequent semen sampling dates, the activity of the analysed enzyme increased to reach 7.72mU/million spermatozoa. In the extracts obtained from the semen collected at the end of the breeding season of arctic foxes, the acrosin activity again reached a value obtained at the beginning of the season.

P–011 Automated sperm morphology assessment using artificial intelligence technology

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Agarwal ◽  
M K Panne. Selvam
Keyword(s):  
Artificial Intelligence ◽  
Predictive Value ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Ease Of Use ◽  
Computer Assisted ◽  
Diagnostic Use ◽  
Normal Sperm

Abstract Study question Can LensHooke X1 PRO semen analyzer be used to evaluate sperm morphology in men with infertility? Summary answer Morphology results generated by X1 PRO are highly reliable when normal sperm forms are ≥4% and therefore they can be reported in such cases . What is known already Most laboratories rely on manual evaluation of sperm morphology smears, which is a time-consuming procedure and its results are subjected to a relatively high variability. However, in recent years the computer-assisted semen analyzers are being increasingly used to evaluate sperm morphology. The X1 PRO semen quality analyzer was designed for in vitro diagnostic use to analyze sperm concentration, total, progressive and non-progressive motility as well as sperm morphology based on WHO 5th edition criteria. Evaluation of sperm morphology using X1 PRO based on AIOM (Artificial Intelligence Optical Microscopic)-based technology requires no fixation steps or staining unlike the manual method. Study design, size, duration This cross-sectional study used 31 semen samples from 8 normozoospermic healthy volunteers and 5 infertile men with a minimum abstinence period between 2 - 3 days. While the 8 healthy semen donors produced a total of 26 ejaculates, which were split into 88 aliquots, the 5 infertile patients produced 5 ejaculates that were split into 13 aliquots. Participants/materials, setting, methods A total of 101 aliquots were prepared from the native semen samples either by dilution or concentration using seminal plasma of the respective donors. Automated semen analysis was performed by the X1 PRO semen analyzer and the results of sperm morphology were compared with manual morphology results using Diff-Quik staining. Statistical analysis was carried out to calculate the positive predictive value (PPV) and negative predictive value (NPV) of X1 PRO semen analyzer. Main results and the role of chance The X1 PRO sperm morphology results show a weak non-significant (P = 0.2441) correlation (r = 0.119) with the manual results. However, X1 PRO demonstrated a high PPV (97.7%) and a low NPV (9.1%) for correct assessment of sperm morphology (≥4%) when compared to manual results. Due to its high PPV, laboratories can report the morphology results generated by X1 PRO in all such cases when normal sperm forms are ≥4%. However, a manual evaluation is necessary in patients with abnormal morphology (&lt;4%). Limitations, reasons for caution One of the limitation of this study is that X1 PRO morphology values did not correlate with manual results. The low NPV seen in our study is due to the inclusion of very few samples with abnormal sperm forms (&lt;4%) in the analysis. Wider implications of the findings: The X1 PRO’s combination of speed, ease of use, accuracy and portability makes it a good choice of device for small medical offices to large IVF centers. High PPV of X1 PRO allows it to correctly identify normal sperm forms for diagnostic use. Trial registration number 18–771

scholarly journals Influence of Fruit and Vegetable Consumption on Antioxidant Status and Semen Quality: A Cross-Sectional Study in Adult Men

2021 ◽  
Vol 8 ◽  
Author(s):  
Dawid Madej ◽  
Dominika Granda ◽  
Ewa Sicinska ◽  
Joanna Kaluza
Keyword(s):  
Antioxidant Status ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Vegetable Consumption ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Fruit And Vegetable Consumption ◽  
Fruit And Vegetables ◽  
Fruit And Vegetable ◽  
Sperm Tail

The influence of fruit and vegetable consumption on semen quality by reducing oxidative stress is inconsistent. Thus, the association between the consumption of these products, antioxidant status, and semen quality was investigated in 90 men aged 18–40. The consumption of fruit and vegetables was collected using the 3-day food record method. Antioxidant status: total antioxidant capacity in semen (TAC-s) and blood (TAC-b), blood superoxide dismutase (SOD-b), glutathione reductase (GR-b), glutathione peroxidase (GPx-b), catalase (CAT-b) activity, and malondialdehyde concentration in blood (MDA-b) were measured. Sperm concentration, leukocytes in the ejaculate, vitality, motility, and sperm morphology were examined using computer-aided semen analysis (CASA). The consumption of fruit and vegetables was positively correlated with sperm concentration, vitality, motility, TAC-s, TAC-b, and SOD-b activity. The TAC-s and TAC-b were positively related to motility, TAC-s was inversely correlated with sperm tail defects. The SOD-b activity was positively correlated with vitality, motility, sperm morphology, and inversely with sperm tail defects and leukocytes in the ejaculate. Compared to the men in the first quartile of fruit and vegetable consumption (&lt;318 g/day), those in the highest quartile (&gt;734 g/day) had the highest sperm concentration, vitality, motility, TAC-s, TAC-b, GPx-b activity, and the lowest MDA-b concentration (based on multivariate regression models). A high consumption of fruit and vegetables may positively influence selected sperm quality parameters by improving the antioxidant status of semen and blood.

scholarly journals ASSOCIATIONS BETWEEN PITUITARY-GONADAL AXIS PARAMETERS AND SEMEN QUALITY IN YOUNG, HEALTHY MEN (AndroLS)

2019 ◽  
Vol 15 (3) ◽  
pp. e11-e24
Author(s):  
Józków Paweł ◽  
Medras Marek ◽  
Szmigiero Leszek ◽  
Słowinska-Lisowska Małgorzata ◽  
Lwow Felicja
Keyword(s):  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Free Testosterone ◽  
Quality Parameters ◽  
Sex Hormone Binding Globulin ◽  
Total Testosterone ◽  
Semen Volume ◽  
The Impact

Background and ObjectiveHormonal measurements play an important role in the evaluation of male fertility potential. In men without hypogonadism, the impact of androgen status on semen is rarely analyzed.Material and MethodsWe evaluated associations between parameters of the pituitary-gonadal axis: luteinizing hormone (LH), follicle-stimulating hormone (FSH), total testosterone (T), free testosterone (FT), calculated testosterone (TC), bioavailable testosterone (TB), sex hormone-binding globulin (SHBG), free androgen index, and semen quality parameters in healthy young men.ResultsIn our study group, sperm concentration and sperm count were associated with FSH and FT. The percentage of immotile sperm was associated with LH and T. The percentage of vital sperm was negatively related to LH. We identified negative, independent associations between T and semen volume (p<0.026) and TC and semen volume (p<0.025). We observed negative, independent association between FSH, FT, and total sperm count (both P<0.002) and between FT and the percentage of normal forms (P<0.012). There were positive associations between LH and T and the percentage of immotile sperm (P<0.007 and P<0.034, respectively). There were no relationships between sperm morphology parameters and the parameters of the pituitary-gonadal axis.ConclusionIn healthy eugonadal men, variations in FSH, LH, T, and FT (within normal limits) are reflected in semen characteristics but not in sperm morphology features.

scholarly journals Effect of L-Carnitine Supplementation on Boar Semen Quality

2007 ◽  
Vol 76 (4) ◽  
pp. 595-600 ◽  
Author(s):  
E. Jacyno ◽  
A. Kołodziej ◽  
M. Kamyczek ◽  
M. Kawęcka ◽  
K. Dziadek ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Morphological Changes ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Carnitine Supplementation ◽  
Boar Semen ◽  
Positive Effect ◽  
Plasma Activity

The effect of addition of L-carnitine on boar semen quality was studied in 5 Pietrain boars at age 1.5 - 2.0 years. The boars received 500 mg of L-carnitine per day for 5 weeks. During this period, their ejaculates were collected once a week and evaluated for quality. The control ejaculates had been collected before the application of L-carnitine. It was found that the addition of L-carnitine to the boars' feed had a positive effect on the quality of boar semen. The total ejaculate volume and sperm-rich fraction volume increased by 11% and 10%, respectively; the total ejaculate sperm count increased by 11.5% (P < 0.05). Also, the number of spermatozoa with major and minor morphological changes decreased and seminal plasma activity of AspAT was significantly reduced (P < 0.01). Sperm concentration and motility, as well as normal acrosome sperm percentage, did not increase considerably. The positive effect of L-carnitine on boar semen quality was observable as early as after one week of its application.

scholarly journals Semen quality trends in French regions are consistent with a global change in environmental exposure

Reproduction ◽  
2014 ◽  
Vol 147 (4) ◽  
pp. 567-574 ◽  
Author(s):  
Joëlle Le Moal ◽  
Matthieu Rolland ◽  
Sarah Goria ◽  
Vérène Wagner ◽  
Perrine De Crouy-Chanel ◽  
...  
Keyword(s):  
General Population ◽  
Global Change ◽  
Environmental Exposure ◽  
Time Trends ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Temporal Model ◽  
Spatio Temporal ◽  
Almost All

A retrospective study carried out recently in a large sample of men, close to the general population, has reported a significant and strong decline in sperm concentration and morphology in the whole of France between 1989 and 2005. We studied these trends within each region of France. Data were obtained from the Fivnat database. The study sample comprised male partners of sterile women in whom both tubes were absent or blocked. They were located at the assisted reproductive technology center. A Bayesian spatio-temporal model with parametric time trends, adjusted for age, was used to model overall time trends for each region. The results show that sperm concentration decreased in almost all regions of France. Among them, Aquitaine showed the highest decrease and Midi-Pyrénées had the lowest average for the whole period. Regarding total motility, most regions showed a slight increase while Bourgogne showed a steep and significant decrease. While considering sperm morphology, there was a decrease in most of the regions. The decrease in Aquitaine and Midi-Pyrénées was stronger when compared with the overall trend. In conclusion, a decrease in sperm concentration and morphology, already shown at the French metropolitan territory level, was observed in most regions of France. This is consistent with a global change in environmental exposure, according to the endocrine disruptor hypothesis especially. Indeed, ubiquitary exposure to chemicals has been growing in the general population of France since the 1950s, and the results do not appear to support the lifestyle hypothesis. The highest decreases and lowest values are consistently observed in two proximate regions that are both highly agricultural and densely populated.

scholarly journals Clinical and Structural Features of Sperm Head Vacuoles in Men Included in the In Vitro Fertilization Programme

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Nina Fekonja ◽  
Jasna Štrus ◽  
Magda Tušek Žnidarič ◽  
Katja Knez ◽  
Eda Vrtacnik Bokal ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Human Sperm ◽  
Structural Features ◽  
Fertilization Rate ◽  
Sperm Head ◽  
Vitro Fertilization

The human sperm head vacuoles and their role in male infertility are still poorly understood. The aim of this study was to identify the clinical and ultrastructural features of human sperm head vacuoles in men included in the in vitro fertilization programme: men with normal (normozoospermia) and impaired sperm morphology (teratozoospermia). The sperm samples were observed under 6000-time magnification using motile sperm organelle morphology examination (MSOME). The proportion of sperm with head vacuoles was evaluated and related to the outcome of in vitro fertilization. The sperm of men with impaired sperm morphology was characterized by a higher proportion of sperm head vacuoles. The sperm head vacuoles were related to impaired semen quality (sperm concentration, motility, and morphology) but were not influenced by male factors (semen volume, height, age, weight, or body mass index). Moreover, sperm head vacuoles were related to impaired fertilization rate merely after classical in vitro fertilization (IVF), while there was no relation to pregnancy. In a subgroup of men, the sperm was fixed and observed by transmission electron microscopy (TEM). The ultrastructural study revealed that sperm head vacuoles are large nuclear indentations of various sizes and positions, packed with membranous material organized in membrane whorls (MW).

scholarly journals Urinary Bisphenol A, F and S Levels and Semen Quality in Young Adult Danish Men

2021 ◽  
Vol 18 (4) ◽  
pp. 1742
Author(s):  
Thea Emily Benson ◽  
Anne Gaml-Sørensen ◽  
Andreas Ernst ◽  
Nis Brix ◽  
Karin Sørig Hougaard ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Male Fertility ◽  
Parental Education ◽  
Negative Binomial ◽  
Semen Quality ◽  
Sperm Count ◽  
Smoking Status ◽  
Sperm Concentration ◽  
First Trimester ◽  
Negative Binomial Regression Model

Bisphenol A (BPA) is considered an endocrine disruptor and has been associated with deleterious effects on spermatogenesis and male fertility. Bisphenol F (BPF) and S (BPS) are structurally similar to BPA, but knowledge of their effects on male fertility remains limited. In this cross–sectional study, we investigated the associations between exposure to BPA, BPF, and BPS and semen quality in 556 men 18–20 years of age from the Fetal Programming of Semen Quality (FEPOS) cohort. A urine sample was collected from each participant for determination of BPA, BPF, and BPS concentrations while a semen sample was collected to determine ejaculate volume, sperm concentration, total sperm count, sperm motility, and sperm morphology. Associations between urinary bisphenol levels (continuous and quartile–divided) and semen characteristics were estimated using a negative binomial regression model adjusting for urine creatinine concentration, alcohol intake, smoking status, body mass index (BMI), fever, sexual abstinence time, maternal pre–pregnancy BMI, and first trimester smoking, and highest parental education during first trimester. We found no associations between urinary bisphenol of semen quality in a sample of young men from the general Danish population.

scholarly journals Expression of Apoptosis-Related Genes in Cat Testicular Tissue in Relation to Sperm Morphology and Seasonality—A Preliminary Study

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 489
Author(s):  
Sylwia Prochowska ◽  
Agnieszka Partyka ◽  
Wojciech Niżański
Keyword(s):  
Sperm Morphology ◽  
Semen Quality ◽  
Testicular Tissue ◽  
Reproductive Season ◽  
Tissue Samples ◽  
Protein Levels ◽  
Expression Of Genes ◽  
Testicular Regression ◽  
Preliminary Study ◽  
Apoptotic Genes

Apoptosis is a crucial process in spermatogenesis, responsible for the elimination of abnormal sperm cells and testicular regression out of breeding season. The aim of this study was to assess if the expression of apoptosis-related genes in testicular tissue of domestic cats differed: (1) between normozoospermic and teratozoospermic donors, and (2) between reproductive and non-reproductive season. The expression of genes: BCL2L1, BCL2, BAX, BAD, FAS, FASLG, and caspases (CASP3, CASP8, CASP9, and CASP10) was analyzed by qRT-PCR in testicular tissue samples. During non-reproductive season significantly higher expression of two anti-apoptotic genes (BCL2L1 and BCL2) was observed. Additionally, there was a significant higher expression of CASP10 in teratozoospermic cats during non-reproductive than during reproductive season. No differences were noted between normozoospermic and teratozoospermic groups. Upregulation of some genes during the non-reproductive season indicates engagement of apoptotic mechanisms in the seasonal changes of semen quality in cats, however further studies on protein levels and analysis of changes on distinct testicular germinal layers are required. At the same time, teratozoospermia in the general population of cats seems to be not connected with dysregulation of apoptosis in the testes.

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