White Tea is More Effective in Preservation of Bone Loss in Adult Rats Co-Exposed to Lead and Cadmium Compared to Black, Red or Green Tea

AbstractLead (Pb) and cadmium (Cd) are toxic metals occurring commonly in the human environment that show mutagenic, genotoxic and carcinogenic effects. Dietary components could prevent heavy metals intoxication by reducing their accumulation in the body. The purpose of the study was to check possible protective effect of regular consumption of white, black, red, or green tea on bone metabolism during long-term exposure to Pb and Cd in adult rats. The 12 week-long exposure to Pb and Cd (50 mg Pb and 7 mg Cd/kg of the diet) in a rat model was studied. Twelve-week-old adult male Wistar rats were randomly divided into a negative control group (Pb and Cd exposure without tea), a control (without Pb and Cd and teas), and groups co-exposed to Pb and Cd and supplemented with green, red, black, or white tea (n=12 each group). The experiment lasted for 12 weeks. The co-exposure to Pb and Cd led to the increase of bone resorption depending on the tea treatment, which was confirmed by the mechanical testing and histomorphometrical examination of cancellous bone. Pb and Cd influenced mechanical strength, reduced the densitometric and geometric parameters and the thickness of growth plate and articular cartilages. Concluding, white tea exerted the best protective effect on bone tissue and hyaline cartilage against heavy metal action.

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Multi Floral Honey Has a Protective Effect against Mammary Cancer Induced by 7,12-Dimethylbenz(a)anthracene in Sprague Dawley Rats

Journal of Agricultural Science ◽

10.5539/jas.v9n2p196 ◽

2017 ◽

Vol 9 (2) ◽

pp. 196 ◽

Cited By ~ 1

Author(s):

Hamed R. Takruri ◽

Maha S. Shomaf ◽

Saida F. Shnaigat

Keyword(s):

Protective Effect ◽

Mammary Cancer ◽

Histopathological Examination ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Light Cycle ◽

Sprague Dawley ◽

Dark Light

This research was conducted to study the protective effect of bee honey on the 7,12-dimethylbenz(a)anthracene (DMBA)- induced breast cancer in rat model. The study consisted of three groups: honey group, positive control group (PC), and negative control group (NC) to which the carcinogen was not administered. All rats were fed the diet recommended by the American Institute of Nutrition for growing rats (AIN-93G), with addition of honey (50 g/kg diet) to the honey group. All Rats were fed their diets ad libitum on 12 hours dark/light cycle. At the age of 50 days all rats in the honey and PC groups were gavaged once by the carcinogen DMBA with a dose of 80 mg/kg body Wt. After three weeks of carcinogen administration, rats were palpated weekly to detect any tumor growth. After 18 weeks, all rats were sacrificed. The palpable structures and the mammary glands along with associated lymph nodes were removed and fixed in saline formalin and prepared for histopathological examination. The results revealed that the honey group diet significantly (p < 0.05) reduced the incidence rate of mammary cancer, palpable tumor multiplicity, tumor size and weight compared to the PC group. In conclusion, multi floral honey has a protective effect against DMBA- induced mammary cancer in the initiation, promotion, and progression stages of DMBA-induced mammary carcinogenesis. However, further research is needed to reveal the mechanisms that might have contributed to the preventive effect of honey against mammary cancer.

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Protective Effect of Combination Commercial Black Seed Oil (Nigella sativa) and Honey Against Cisplatin-Induced Hepatotoxicity in Rats

Muhammadiyah Medical Journal ◽

10.24853/mmj.1.2.43-48 ◽

2020 ◽

Vol 1 (2) ◽

pp. 43

Author(s):

Andri Muhrim Siddiq ◽

Muhammad In'am Ilmiawan ◽

Mitra Handini

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Rat Liver ◽

Liver Tissue ◽

Seed Oil ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Black Seed ◽

Black Seed Oil

Background: The chemotherapeutic use of cisplatin (CP) is restricted because of its hepatotoxicity induced by oxidative stress. Malondialdehyde (MDA) is a secondary product of lipid peroxidation as a biomarker of oxidative stress. Individual administration of black seed oil (BSO) or honey (H) demonstrated hepatoprotective effect in rats. Interaction of both substances when administrated as combination can be evaluated using combination index (CI) to quantitatively depict synergism (CI<1), additive (CI=1) and antagonism effect (CI>1). Objective: to know the combination effect of BSO and honey on rat liver tissue given CP exposure. Methods: This study used 30 rats were divided into 10 groups. Normal group (N); Negative control group (NC); P1-P4 groups were administerated BSO (1 and 2 mL/kg) and honey (3.7 and 7.4 mL/kg); P5-P8 groups were combination of BSO and H. P1-P8 groups were given BSO and honey orally for 21 days. On the 18th day, NC and P1-P8 groups were given CP 8 mg/kg intraperitoneally, while the N group was given NaCl 0.9% 1 mL/kg intraperitoneally. Result: Malondialdehyde (MDA) levels were found to be lower in P1-P8 groups compared to negative control group and P6 and P7 groups have levels equivalent to MDA levels of normal control group (p > 0.05). Conclusion: Combination of BSO and honey provides a protective effect on cisplatin-induced rat liver tissue damage indicated by reduced MDA levels, but all combination group showed antagonism effect.

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Ameliorative Effect of Ethanolic Extract of Petroselinum Crispum Against Gentamicin-Induced Nephrotoxicity in Rabbits Male

Al-Anbar Journal of Veterinary Sciences ◽

10.37940/ajvs.2020.13.2.5 ◽

2020 ◽

Vol 13 (2) ◽

Author(s):

Sarah Hussain ◽

Mariam Kadhem

Keyword(s):

Body Weight ◽

Clinical Signs ◽

Ethanolic Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

The Body ◽

Positive Control Group ◽

Control Group ◽

Petroselinum Crispum

The experiment was conducted to investigate the protective effect of Petroselinum crispum leave extracted against gentamicin-induced nephrotoxicity in male rabbits by studying the body weight, clinical signs, haematological and biochemical parameters, gross lesion and histopathological changes. Twenty four rabbits male were used and divided into 4 groups. Group 1: rabbits served as a negative control, received distilled water 1 ml(orally). Group 2: rabbits served as a positive control group, received gentamicin at a dose of 80 mg/kg/day intramuscular for 15 days. Group 3: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 125 mg/kg orally for 15 days. Group 4: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg orally for 15 days.The results of the gentamicin treated group( positive control group) showed clinical signs such as loss of body weight, loss of appetite and rough hair with hematuria. The body weight a significantly declined (p≤ 0.05) compared other groups. There was a significant decrease (p≤ 0.05) in WBC count, lymphocyte, GSH, SOD, CAT, and GPX levels, while it recorded a significant increase (p≤0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA. Histological studies showed several kidney pathological changes such as pale colour, enlargement in size and weight and easy from detaching as opposed to negative control group. On the other hand, the group treated with ethanolic extractof Petroselinum crispum at dose 125 mg/kg induced improved of parameters as recorded significant increased(P ≤ 0.05) in body weight, WBC count, lymphocyte, GSH, SOD, CAT, and GPX, while significant decreased (P ≤ 0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA compared with the positive control group whereas rabbits treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg restored the parameters and histological changes of the kidney to near normal status compared with the negative control group. These results showed that the dose-detected Petroselinum crispum extract (250mg / kg) acts as potential curative effect against gentamicin-induced nephrotoxicity in male rabbits.

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POTENTIAL ETHANOL OF Carica papaya L. EXTRACT AS IMMUNOMODULATORY THROUGH HISTOLOGY OBSERVATION AT MICE BALB/C SPLEEN

Berkala Kedokteran ◽

10.20527/jbk.v13i2.4077 ◽

2017 ◽

Vol 13 (2) ◽

pp. 205

Author(s):

Sri Tasminatun ◽

Ria Pravitasari ◽

SN. Nurul Makiyah

Keyword(s):

Treatment Group ◽

Carica Papaya ◽

Negative Control Group ◽

Negative Control ◽

The Body ◽

Control Group ◽

Control Group Design ◽

Post Test ◽

One Way Anova

Abstract: The diameter of the alba pulp of the spleen will be enlarged, when the body responds to allergens by increasing the activity of the immune system. Papaya fruits (Carica papaya L.) containing flavonoid that can be as an immunomodulator. This study aims to determine the effect of C. papaya extract to the diameter size alba pulp of the spleen of Ovalbumin (OVA) induced Balb/C mice. The research design was experimental in vivo animal test with post-test only control group design. Thirty male Balb/C mice, ±20grams weight, 8 weeks old, divided into six groups consists of a normal control, negative control, Methylprednisolone, and C. papaya extract treatment group (175mg/kg, 350mg/kg and 700mg/kg dose per day). The extract and Methylprednisolone are given for 28 days, while OVA is given on 15thand 22ndday (intraperitoneal,) and 23rd until 28thday (peroral). On the 29thday the mice are sacrificed and the duodenum was made into histological preparation with HE staining. The immunomodulator effect is assessed by the average of the diameter size alba pulp of the spleen under a light microscope. The data were analyzed using SPSS15 with one way ANOVA continued with Tukey test. The average of the diameter size alba pulp of the spleen for control group is 10,165 ± 0,195; negative control group is 30,357 ± 0,826; methylprednisolone group is 10,220 ± 0,346; 175mg/kgbb dose extract is 14,956 ± 1,057; 350mg/kgbb dose extract is 14,030 ± 0,667; and 700mg/kgbb dose extract is 12,504 ± 0,729. The conclusion of this study is Carica papaya L. extract treatment group (175mg/kg, 350mg/kg and 700mg/kg dose per day) has an effect to the average of the diameter size alba pulp of the spleen in OVA-induced Balb/C mice significantly. 700mg/kg dose per day has the same effect with the methylprednisolone group. Keywords: immunomodulator, Carica papaya L., alba pulp of the spleen, ovalbumin

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Green Coffea robusta (Coffea canephora) from Lampung province effect toward free radicals in chickens infected with Salmonella enteritidis bacteria

Open Veterinary Journal ◽

10.4314/ovj.v11i1.10 ◽

2021 ◽

Vol 11 (1) ◽

pp. 61-69

Author(s):

Dahliatul Qosimah ◽

Djalal Rosyidi ◽

Lilik E. Radiati ◽

Indah A. Amri ◽

Dodik Prasetyo ◽

...

Keyword(s):

Salmonella Enteritidis ◽

Pathogenic Bacteria ◽

Negative Control Group ◽

Coffea Canephora ◽

Positive Control ◽

Negative Control ◽

The Body ◽

Control Group ◽

High Dose ◽

Treatment Groups

Background: Foodborne diseases are caused by acquired pathogenic bacteria such as Salmonella enteritidis. It causes an intestinal imbalance and the microbial toxins found in the gastrointestinal tract induce symptoms such as diarrhea. Coffee contains active ingredients such as antioxidants and is used as an anti-inflammatory agent by reducing pro-inflammatory cytokine levels in the body. Aim: The purpose of this study was to determine the interaction between Lampung’s robusta coffee and tissue damage in chickens infected by S. enteritidis. Methods: This study used first-day-old Isa brown layer chickens (n = 60), which were divided into five treatment groups. The negative control group consisted of healthy and normal chickens, whereas the positive control group consisted of chickens infected with S. enteritidis bacteria at a concentration of 108 CFU/ml. Groups T1, T2, and T3 were given coffee extract with doses of 500 mg/kg BW (low dose), 1,000 mg/kg BW (moderate dose), and 1,500 mg/kg BW (high dose), respectively, and then infected with S. enteritidis bacteria at a concentration of 108 CFU/ml. The coffee extract and bacteria were given orally via a feeding tube at a volume of 0.5 ml per chick. The extract was given for 14 days (from day 3 to day 16), and the bacteria were given on days 16 and 17. On day 18, the chickens were necropsied. The malondialdehyde (MDA) level was analyzed using one-way analysis of variance test with the GLM procedure (<0.05), while the tissue histopath was analyzed using a descriptive qualitative study to examine the ileal damage Results: The results showed that the MDA levels (nmol/l) decreased in treatment groups T1, T2, and T3 compared to the positive control. On the contrary, we found improvements in the ileum histopathology of group T1 and T2 in the form of normal and regular intestinal epithelium arrangement of the ileum, long intestinal villi, and decreased total leukocytes. Conclusion: Green coffee robusta has the potential to increase antioxidants and reduce inflammation in the small intestine of chickens infected with S. enteritidis.

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Protective effect of total flavonoids from boxthorn leaf against UVB irradiation-induced skin injury

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v18i9.23 ◽

2021 ◽

Vol 18 (9) ◽

pp. 1943-1947

Author(s):

Qing Yu ◽

Ying Shen ◽

Yadan Gan ◽

Liang Zheng

Keyword(s):

Protective Effect ◽

Negative Control Group ◽

Negative Control ◽

Ultraviolet B ◽

Control Group ◽

Total Flavonoids ◽

Skin Damage ◽

Skin Injury ◽

Uvb Irradiation ◽

Base Group

Purpose: To investigate the protective effect of total flavonoids from boxthorn leaf against skin injury induced by UVB irradiation, and to elucidate the underlying mechanism of action. Method: Healthy female mice (n = 100) were randomly divided into four groups: normal control group, UV negative control group, cream base group, and boxthorn leaf total flavonoid (BLTF) group, with 25 mice in each group. The mice in each group were irradiated with ultraviolet B (UVB) irradiation instrument for 1.5 h daily for 3 weeks. Mice in the cream base group were smeared with cream base on their backs, while mice in BLTF group were smeared with 15 mg/g boxthorn BLTF cream. The control and negative control group mice were not treated. Changes in superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) levels were determined using standard methods. Results: Compared to the negative control group, the levels of SOD and GSH-Px in the control group and BLTF were significantly elevated, while MDA levels declined significantly (p < 0.05). Although higher GSH-Px and SOD levels, and lower MDA were seen in the cream base group than in negative control group, these indices were comparable for the two groups (p > 0.05). Conclusion: The total flavonoids of boxthorn leaves improve resistance to UVB-induced skin damage by regulating SOD, MDA and GSH-Px levels in the skin of mice. Thus, they exert protective effects on the skin.

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Anti-diabetic Potential of Aqueous Extract of Moringa oleifera, Ocimum gratissimum and Vernonia amygdalina in Alloxan-Induced Diabetic Rats

10.1101/2020.08.09.242941 ◽

2020 ◽

Author(s):

A. L. Ayobami ◽

E. A. Kade ◽

K. A. Oladimeji ◽

S. Kehinde ◽

K. Gurpreet

Keyword(s):

Body Weight ◽

Aqueous Extract ◽

Fasting Blood Glucose ◽

Negative Control Group ◽

Negative Control ◽

Diabetic Rats ◽

The Body ◽

Control Group ◽

Vernonia Amygdalina ◽

Ocimum Gratissimum

AbstractThe incidence of diabetes mellitus (DM) is increasing globally and it is a major source of concern. This study was undertaken to assess the antidiabetic effect of the aqueous extract of Moringa oleifera, Ocimum gratissimum and Vernonia amygdalina. Sixty adult Wistar rats with body weight of 120-150 g were randomly assigned to groups of five rats each (n=12). Groups 1 served as normal control; Groups 2-5 were diabetic groups; group 2 served as negative control; group 3-5 received 100, 200 and 400 mg/kg of triherbal formula respectively. The body weight (BW) and fasting blood glucose level (FBSL) of the rats were monitored weekly. At the end of the experiment, all the rats were anaesthetized intraperitoneally (I.P) and blood samples were collected by cardiac puncture for biochemical analysis. There was an increase in the BW of the control group and varying doses of tri-herbal formation. It caused 88.0% decrease in FBSL; 371.7%, 386.6% and 296.0% with respect to 100, 200 and 400 mg/kg. Sub-chronic study of the effect of the extract showed a significant increase (P<0.05) in packed cell volume (PCV), white blood counts in rat induced diabetes. The histological studies showed that the diabetic rats with the architecture of the pancreas distorted, was restored to normal by the extract. Its LD50 was found to be greater than 1000 mg/kg indicating its safety in rats. This study has shown that triherbal formula has hypoglycemic and haematogical effects.

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Protective effect of Shenfu on gut epithelium in a porcine model of hemorrhagic shock

Journal of Investigative Medicine ◽

10.1136/jim-2021-001939 ◽

2021 ◽

pp. jim-2021-001939

Author(s):

Yong Liang ◽

Chunsheng Li ◽

Wei Yuan ◽

Junyuan Wu ◽

Qiang Zhang ◽

...

Keyword(s):

Blood Transfusion ◽

Protective Effect ◽

Hemorrhagic Shock ◽

Porcine Model ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Necrosis Factor Alpha ◽

Fatty Acid Binding ◽

Gut Epithelium

This study aimed to explore the protective effect of Shenfu on the hemodynamics and gut integrity in a porcine model of hemorrhagic shock. Hemorrhagic shock was induced in 32 domestic pigs with a rapid bleeding via the arterial sheath to a mean arterial pressure of 40 mm Hg within 10 min. Animals with hemorrhagic shock were then randomly assigned into the negative control group (n=8), receiving neither blood transfusion nor drug treatment; the blood transfusion group, in which animals were given blood transfusion alone; the saline group, in which animals were blood transfused and resuscitated with saline (3 mL/kg); and the Shenfu group, in which animals received blood transfusion and resuscitation with Shenfu (3 mL/kg). Blood tumor necrosis factor-alpha (TNF-ɑ) and interleukin-6 were measured using ELISAs. Tissue levels of superoxide dismutase (SOD), malondialdehyde (MDA), Na+/K+-ATPase, Ca++ATPase, myeloperoxidase (MPO), and fatty acid binding protein 2 (FABP2) were determined using respective quantitation kits. Fluid resuscitation with Shenfu significantly improved HR, CI, and MAP of pig with hemorrhagic shock, which was accompanied with mitigation of tissue damages in intestinal epithelium. Blood TNF-ɑ was reduced in the Shenfu group. Bcl-2 and cleaved caspase-3 expression in intestinal tissues were elevated and decreased, respectively, in pigs treated with Shenfu. Notably, treatment with Shenfu suppressed oxidative stress markers MDA, MPO, and FABP2 in the intestine. Oppositely, SOD, Na+/K+-ATPase and Ca++ATPase levels in intestinal tissues were promoted by Shenfu treatment. Shenfu demonstrates significant protective effect on the hemodynamics and gut epithelium of pigs with hemorrhagic shock.

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Differences in effectiveness of water steeping from green tea leaf and black tea leaf as hemostasis effect on wound cut-tail mice (mus musculus)

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v1i3.310 ◽

2016 ◽

Vol 1 (3) ◽

pp. 163

Author(s):

Hasmawati Hasan ◽

Wenni Raharja

Keyword(s):

Green Tea ◽

Tooth Extraction ◽

Bleeding Time ◽

Black Tea ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Tea Leaves ◽

Green Tea Leaves ◽

Tea Leaf

Tooth extraction is a simple procedure that is frequently performed in the practice of dentistry. Bleeding is a common complications of tooth extraction. There are several materials or methods that may be used to control the bleeding. To minimize side effect, it might need natural ingredients as a replacement. Tannin and flavonoid is a compounds that can reduce bleeding time. Green tea and black tea are plants that have these compounds with different concentrations. Aims of this study is to determine the differences in the effectiveness of water steeping green tea leaves and black tea leaves as a hemostasis effect on wound cut-tail mice (Mus musculus). This is an experimental research with study design post-test only control group design. Samples were 30 male mice which divided into three groups. One negative control group (aquadest) and two treatment group (green tea and black tea). Mice that match the criteria is cutted the tail equal to 3 mm from the tip of the tail, then the ingredients is applied to the wound. Blood is dripped on absorbent paper until bleeding stop and data were analyzed using Kruskal-Wallis. There are significant differences between negative control group and the treatment group. In the treatment groups there were significant differences in the bleeding time between green tea group and black tea group. As a conclusion, water steeping from green tea leaves have a better effect than black leaves tea as hemostasis on wound cut-tail mice.

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Do Different Samples From Pregnant Women and Their Neonates Share the Common Microbiome: A Prospective Cohort Study

10.21203/rs.3.rs-1062191/v1 ◽

2021 ◽

Author(s):

Jee Yoon Park ◽

Huiyoung Yoon ◽

Seung-been Lee ◽

Hyeon Ji Kim ◽

Young Hwa Jung ◽

...

Keyword(s):

Pregnant Women ◽

Strong Association ◽

Alpha Diversity ◽

Negative Control Group ◽

Negative Control ◽

The Body ◽

Control Group ◽

Microbiome Composition ◽

Tertiary Center ◽

A Prospective Study

Abstract Background We characterized the microbiome composition from various samples in pregnant women and their neonates and evaluated the association between the distribution of microbiome and obstetric factors. Results A prospective study was performed in live births delivered at a single tertiary center between March 2020 and January 2021. Samples collected from pregnant women and their newborns included maternal cervicovaginal discharge (VD), amniotic fluid (AF), umbilical cord blood (CB), neonatal gastric liquid (GL), and meconium. Next-generation sequencing was used to analyze the composition of the microbiome from the samples. We identified 19,597,239 bacterial sequences from the 641 samples. The alpha diversity of the negative control group was slightly higher than that of the VD group. The samples were separated well based on the microbiome pattern unique to their body sites despite the significant batch effects present within our dataset. We observed a higher relative abundance of Lactobacillus at the genus level but lower abundances of Aerococcus, Fusobacterium, Gardnerella, Peptoniphilus, Porphyromonas, and Prevotella in VD. In evaluating the association with clinical data, Staphylococcus in M showed a strong association with preterm birth. The lists of bacterial taxa were connected to the weights of newborn babies in the CB group. The microbiome pattern of GL was similar to that of AF, but not technically identical. Twins did not demonstrate significant differences in the composition of microbiomes from neonate samples. Conclusions In this study, samples known to be traditionally sterile, such as AF and CB, showed specific bacterial taxa although they were extremely close to negative controls. The microbiome patterns were grouped according to the body compartments where the samples had been obtained, not according to the mother-fetus/infant pairs. Further studies lowering the possibility of contamination and redeeming the low-biomass specimens are necessary to determine the relationship between microbiomes and specific pathological complications during pregnancy.

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