Influence of Drying Temperature on the Content of Bioactive Compounds in Scots Pine (Pinus sylvestris L.) Shoots as Well as Yield and Composition of Essential Oils

2020 ◽  
Vol 24 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Natalia Matłok ◽  
Józef Gorzelany ◽  
Tomasz Piechowiak ◽  
Maciej Balawejder
Keyword(s):  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Vitamin C ◽  
Pinus Sylvestris ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Scots Pine ◽  
Process Conditions ◽  
Drying Process ◽  
Chromatography Method

AbstractThe study concerns the effects of drying process conditions (40, 50, 60°C) on the contents of bioactive compounds in Scots pine (Pinus sylvestris L.) shoots as well as the yield and composition of essential oils. The obtained dry material was examined for the total contents of polyphenols and the antioxidant activity as well as the content of vitamin C. The material was examined by using the HS-SPME method, and the composition of the essential oils was determined using gas chromatography method (GC-MS). The highest contents of bioactive compounds were found in the material subjected to drying at 40°C. The highest yield of essential oils (0.63 ml 100 g−1 d.m. of the material) was obtained from the pine shoots dried at 40°C.

scholarly journals Impact of Ozonation Process on the Content of Bioactive Compounds with Antioxidant Properties in Scots Pine (Pinus sylvestris L.) Shoots as Well as Yield and Composition of Essential Oils

2020 ◽  
Vol 24 (2) ◽  
pp. 146-155
Author(s):  
Natalia Matłok ◽  
Józef Gorzelany ◽  
Tomasz Piechowiak ◽  
Piotr Antos ◽  
Miłosz Zardzewiały ◽  
...  
Keyword(s):  
Vitamin C ◽  
Pinus Sylvestris ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Antioxidant Properties ◽  
Raw Material ◽  
Volatile Fraction ◽  
Polyphenol Content ◽  
The Impact ◽  
Ozonation Process

AbstractThis study examined the impact of the ozonation procedure on the content of bioactive compounds in young shoots of Pinus sylvestris L. as well as on the yield and the composition of the essential oils and volatile fraction. The ozonation process consisted of exposure of the raw material during 5, 15 and 30 min to the ozone gas with the concentration of 10 and 100 ppm, and then it was stored for 24 hours. In the ozonated pine shoots, the content of vitamin C overall content of polyphenol content and antioxidant potential were determined. It was found that the investigated parameters increased as a result of the utilized procedure. The observed increase in the content of tested bioactive compounds was dependent on exposition time and was found to be highest for ozonated material for 15 min with ozone gas at a concentration of 10 ppm. As a result of the ozonation procedure the content of polyphenolic compounds and vitamin C content increased. Whereas it, did not have a significant effect on composition of the obtained essential oils. It was found that ozone is an effective elicitor, which allows increasing the content of bioactive compounds in pine shoots.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DARI EKSTRAK ETANOL SPONS Ianthella basta DARI DESA TUMBAK KECAMATAN PUSOMAEN KABUPATEN MINAHASA TENGGARA

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 713
Author(s):  
Nurhati Anton ◽  
Adithya Yudistira ◽  
Jainer Pasca Siampa
Keyword(s):  
Antioxidant Activity ◽  
Coral Reef ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Ethanol Extract ◽  
Positive Control ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
Ethanol Extracts

ABSTRACT Sponges are a component of coral reef biota that has bioactive compounds with a greater percentage of activity compared to compounds produced by terrestrial plants. This study aims to determine the presence of antioxidant activity in the ethanol extracts of sponge Ianthella basta from the waters of Tumbak Village. Ianthella basta sponge was extracted using maceration method with 96% ethanol solvent. Antioxidant activity testing was carried out using the DPPH method with a concentration of 100 µg / mL, 75 μg / mL, 50 μg / mL, and 25 μg / mL and Vitamin C p.a as positive control. Each sample was made three repetitions of the test. Testing using a UV-Vis spectrophotometer. The results showed that the ethanol extract of the Ianthella basta sponge had antioxidant activity with a percentage of 48.73% at a concentration of 100 µg / mL. Keywords: Ianthella basta sponge, Antioxidants, Extraction, DPPH  ABSTRAK Spons merupakan salah satu komponen biota penyusun terumbu karang yang mempunyai senyawa bioaktif dengan persentase keaktifan lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan di dalam ekstrak etanol Spons Ianthella basta dari perairan Desa Tumbak. Spons Ianthella basta diekstraksi menggunakan metode maserasi dengan pelarut etanol 96%. Pengujian aktivitas antioksidan dilakukan dengan menggunakan metode DPPH dengan konsentrasi 100 µg/mL, 75 µg/mL, 50 µg/mL, dan 25 µg/mL dan Vitamin C p.a sebagai kontrol positif. Masing-masing sampel dibuat tiga kali pengulangan uji. Pengujian menggunakan alat spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa ektrak etanol Spons Ianthella basta memiliki aktivitas antioksidan dengan persentase sebesar 48,73% pada konsentrasi 100 µg/mL. Kata Kunci: Spons Ianthella basta, Antioksidan, Ekstraksi, DPPH

scholarly journals Nutrients and bioactive compounds of pulp, peel and seed from umbu fruit

2019 ◽  
Vol 49 (4) ◽  
Author(s):  
Leilson de Oliveira Ribeiro ◽  
Eliseth de Souza Viana ◽  
Ronoel Luiz de Oliveira Godoy ◽  
Sidinea Cordeiro de Freitas ◽  
Suely Pereira Freitas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Nutritional Composition ◽  
Total Phenolic ◽  
Total Carotenoids ◽  
Fresh Pulp ◽  
Main Barrier ◽  
By Products

ABSTRACT: The objective of this research was to evaluate the nutritional composition and bioactive compounds of whole umbu fruit, including pulp, seed and peel, and also of a commercial umbu pulp. Samples of the fractions and of commercial pulp were analyzed for determination of minerals and proximate composition, total phenolic and antioxidant activity. Pulps and peel were also analyzed for vitamin C and carotenoids contents. Commercial pulp presented better nutritional composition than fresh pulp (P<0.05) and the peel presented higher phenolic content and antioxidant activity than seed. Peel also stood out by its vitamin C (79 mg.100 g-1) and total carotenoids (2,751 µg.100 g-1) contents, showing that, as the main barrier of the fruit for its protection, it is a fraction rich in bioactive compounds. The highest dietary fiber and iron contents were observed in umbu seed (P<0.05). Therefore, umbu by-products may be ingredients proper for development of food richer in nutrients and bioactive compounds.

scholarly journals Ammoides pusilla (Brot.) Breistr. from Algeria: Effect of harvesting place and plant part (leaves and flowers) on the essential oils chemical composition and antioxidant activity

2016 ◽  
Vol 14 (1) ◽  
pp. 343-350
Author(s):  
Choukri Tefiani ◽  
Ali Riazi ◽  
Boumediene Belbachir ◽  
Hicham Lahmar ◽  
Smail Aazza ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Essential Oils ◽  
Reducing Power ◽  
Plant Part ◽  
Gas Chromatography Mass Spectrometry ◽  
Scavenging Activity ◽  
Strong Negative Correlation ◽  
Chemical Evaluation ◽  
Chemical Variability

AbstractThe chemical variability and antioxidant activity of the flower and leaf essential oils (EOs) of Ammoides pusilla, collected at Algeria was evaluated. The EOs were isolated by hydrodistillation and analyzed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS). Antioxidant activity was assessed by DPPH, ABTS, Reducing Power and TBARS assays. Oxygen-containing monoterpenes (54-77%) dominated all but one A. pusilla leaf EOs, and in two flower EOs (53% both). Thymol dominated in practically all leaf and flowers EOs, but cumin alcohol, p-cymene and limonene attained also relatively high percentages in some EOs. A strong negative correlation (p<0.01) between IC50 values of ABTS, DPPH, and hydroxyl scavenging activity and the percentages of p-cymene and cumin alcohol present in EOs were observed, showing that higher levels of these monoterpenes were responsible for the best activities found. In spite of this finding, the antagonism and/or synergism between EO components must be taken into account, since the EO activity can only be considered as a whole. Moreover, given the EOs chemical variability their use as antioxidants, should be preceded by their chemical evaluation.

scholarly journals Influence of drying on bioactive compounds and antioxidant activity of fruits of guabiju (Myrcianthes pungens)

2021 ◽  
Vol 10 (8) ◽  
pp. e5510817024
Author(s):  
Vanessa Barbieri Bombana ◽  
Carolina Elisa Demaman Oro ◽  
Diane Rigo ◽  
Caroline Chies Polina ◽  
Andressa Franco Denti ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Water Activity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Dry Extract ◽  
Influence Of Temperature ◽  
Physical Chemical ◽  
Influence Of Temperature On

The objective of this work was to evaluate the influence of temperature on the content of bioactive compounds of fruits of guabiju (Myrcianthes pungens (O. Berg) D. Legrand). The peel, pulp and seed of fresh guabiju were analyzed in relation to physical-chemical composition, metals, color, phenolic compounds, flavonoids, anthocyanins, vitamin C and antioxidant activity. On the dehydrated samples at temperature of 60 °C, where also determined the moisture and water activity. The fractions of the fruit showed high amounts of metals. After drying, moisture of 1.3, 1.0 and 0.9% were observed for peel, pulp and seed and water activity of 0.44 to 0.54. All the samples darkened, with less variation in the dehydrated peel (∆E 9.2). The samples showed high values of bioactive compounds, and in the fresh peel were observed higher levels of phenolic compounds (8459.8 mg EGA/100g dry extract), anthocyanins (152.0 mg/100 dry extract) and vitamin C (222.9 mg/100g) and on the dehydrated seed higher value of flavonoids (7480.7 mg EQ/100g dry extract). There was 86.3% degradation of anthocyanins in the dehydrated peel. The best values of antioxidant activities were obtained for the dehydrated peel (IC50 1.37 mg/mL), seed (IC50 1.49 mg/mL) and in the fresh peel (IC50 1.41 mg/mL).

scholarly journals Physical, physicochemical, microbiological, and bioactive compounds stability of low-calorie orange jellies during storage: packaging effect

2020 ◽  
Vol 9 (9) ◽  
pp. e759997900
Author(s):  
Hellen Vidal Santos ◽  
Cassandra Justina Souza Maia ◽  
Elismara de Jesus Ferreira Lima ◽  
Ana Clara Costa Dias ◽  
Reginaldo de Souza Monteiro ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Total Phenolic ◽  
Total Sugars ◽  
Low Calorie ◽  
Great Progress ◽  
Useful Life ◽  
Compounds Stability ◽  
Growth Of Fungi

Fruit jellies are widely produced as a way to utilize fresh fruits, which are highly perishable. Orange a fruit widely consumed in Brazil, it has a significant amount of bioactive compounds. Despite the great progress in the development of jellies, several factors can change its useful life, among them is the packaging. Therefore, the objective of this study was to evaluate the effect of packaging on the physicochemical, physical, microbiological and bioactive compounds stability of low-calorie orange jellies during storage. Analyses every 30 days during the 180 days of storage. The results showed that increased storage time led to a decrease in pH, reduction of the flow rate (polypropylene packaging), reduction in yellow intensity, and growth of fungi and yeasts (higher in polypropylene packaging). In contrast, luminosity, red intensity, moisture, total sugars, and the consistency index tended to remain stable during storage. The DPPH results showed an increase in the antioxidant activity and reduction of vitamin C throughout the period of storage, especially in polypropylene packaging. The total phenolic content was stable with a tendency to decrease during storage. Notably, vitamin C showed a positive correlation with antioxidant activity in jellies. Low-calorie orange jellies packaged in glass showed the least changes during storage.

scholarly journals The Antioxidant and Antibacterial Activities of the Essential Oils of Marrubium vulgare Growing Wild in Eastern Algeria

2013 ◽  
Vol 15 ◽  
pp. 159-166 ◽  
Author(s):  
Abderazak Abadi ◽  
Abdellatif Fahima
Keyword(s):  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Marrubium Vulgare ◽  
Eastern Algeria

The chemical composition was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The constituents were identified by their mass spectra and Kovats’ indices. Fivety (50) components in the oil of M. vulgare were identified. The results demonstrated that the major components of the essential oil were: 4,8,12,16-Tetramethyl heptadecan-4-olid (16.97%), Germacrene D-4-ol (9.61%), α- pinéne (9.37%), Phytol (4.87%), Dehydro-sabina ketone (4.12%), Piperitone (3.27%), δ-Cadinene (3.13%), 1-Octen-3-ol (2.35%) and Benzaldehyde (2.31%). The antioxidant activity of the oils was assessed by measurement of metal chelating activity, the reductive potential, the free radical scavenging (DPPH) assay. The antioxidant activity was compared with that of synthetic antioxidants: butylated hydroxyanisole (BHT), and the essential oils. The results showed as the Essential oil of M. vulgare was evaluated for its antibacterial activities against Gram-positive and Gram negative pathogenic bacteria: Listeria monocytogene, Pseudomonas aeruginosa, Agrobacterium tumefaciens and Salmonella enterica.

scholarly journals Effects of the drying process on the fatty acid content, phenolic profile, tocopherols and antioxidant activity of baru almonds (Dipteryx alata Vog.)

2020 ◽  
Vol 71 (1) ◽  
pp. 343
Author(s):  
M. L.L. Campidelli ◽  
J. D.S. Carneiro ◽  
E. C. Souza ◽  
M. L. Magalhães ◽  
E. E.C. Nunes ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Vitamin C ◽  
Fatty Acid Content ◽  
Monounsaturated Fatty Acids ◽  
Coumaric Acid ◽  
Drying Process ◽  
Phenolic Profile ◽  
Dipteryx Alata

This study carried out a chromatographic and spectrophotometric characterization of the bioactive compounds, antioxidants, phenolics, tocopherols, sterols and fatty acids of baru almonds “in natura” and submitted to drying processes. It was determined that baru “in natura” almonds presented high levels of phenolic compounds, vitamin C, antioxidants, phenolics, sterols, total monounsaturated fatty acids and low thrombogenic, and atherogenic indexes. During the process of drying it at 65 °C for 30 minutes, a decrease was noted in the levels of caffeic acid, chlorogenic acid, anthocyanins, p-coumaric acid, ferulic acid, o-coumaric acid, quercetin, and polyunsaturated fatty acids. The same condition resulted in an increase in the levels of gallic acid, rutin, catechin, trans-cinnamic acid, vanillin, m-coumaric acid, tocopherols, monounsaturated fatty acids and antioxidant activity (ORAC and DPPH). When submitted to a temperature of 105 ºC for 30 minutes the same behavior was seen with a reduction in the vitamin C and ORAC contents and increased presence of flavonoids.

scholarly journals Effect of Vacuum Impregnation with Apple-Pear Juice on Content of Bioactive Compounds and Antioxidant Activity of Dried Chokeberry Fruit

Foods ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 108
Author(s):  
Agnieszka Nawirska-Olszańska ◽  
Marta Pasławska ◽  
Bogdan Stępień ◽  
Maciej Oziembłowski ◽  
Kinga Sala ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Water Activity ◽  
Bioactive Compounds ◽  
Polyphenolic Compounds ◽  
Vacuum Pressure ◽  
Vacuum Impregnation ◽  
Drying Process ◽  
Vacuum Technology ◽  
Pear Juice ◽  
Positive Effect

Food technology seeks ways to preserve products while maintaining high bioactive properties. Therefore, an attempt was made to assess the effect of the process of impregnation with apple-pear juice and the drying process on the content of bioactive compounds in chokeberry fruit. Chokeberry fruits were subjected to impregnation with apple-pear juice at three levels of vacuum pressure, 4, 6, and 8 kPa; then, they were dried using microwave-vacuum technology. The water activity of the obtained products, the content of fructose, glucose, sorbitol, and polyphenolic compounds, and antioxidant activity were determined. A total of 20 polyphenolic compounds were identified in the fruits and the obtained products (seven anthocyanins, six flavonols, four phenolic acids, and three flavan-3-ols). Preliminary processing, which consisted of introducing the juice ingredients into tissue of the chokeberry fruit, resulted in increased content of bioactive compounds. Moreover, a positive effect of impregnation on the antioxidant stability of the fruit after drying was noted. Water activity in the obtained products showed their microbiological safety. Impregnation at 4 kPa vacuum pressure proved to be the most desirable; in such conditions, the best product in terms of the content of bioactive compounds was obtained.

CHEMICAL PROFILING OF BUCHANANIA LANZAN SPRENG ESSENTIAL OIL AND ITS BIOLOGICAL ACTIVITIES

2020 ◽  
pp. 103-108
Author(s):  
AJITH S ◽  
KRISHNA V ◽  
RAVI KUMAR S ◽  
VINAY KUMAR NM
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Molecular Docking ◽  
Essential Oil ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Dna Gyrase ◽  
Diffusion Method

Objective: The present study was designed to evaluate the chemical composition of the essential oil of Buchanania lanzan Spreng extracted from the seeds and to evaluate in vitro antimicrobial antioxidants and molecular docking studies of the major bioactive compounds of essential oil. Methods: The essential oil was obtained by hydrodistillation of the B. lanzan seeds and analyzed by gas chromatography-mass spectrometry (GC-MS). Antibacterial activity was evaluated against Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholerae, Staphylococcus aureus, and Streptococcus pneumoniae clinical isolates by disk diffusion method and resazurin assay determined the minimum inhibitory concentration. The in vitro antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging assay; the essential oil major bioactive compounds are Androstan-3-ol, Campesterol, and γ-Sitosterol were docked against bacterial protein DNA gyrase. Results: GC-MS analysis exhibited the presence of 19 bioactive compounds. The essential oil showed that significant antibacterial activity was noticed against V. cholerae and S. typhi with the highest zone of inhibition 15.67–1.20 and 13.83–0.33, respectively. Antioxidant activity in DPPH and H2O2 scavenging assays with IC50 values of 134.23 and 191.24, respectively. The molecular docking of Androstan-3-ol and γ-Sitosterol with bacterial DNA gyrase unveiled a good binding affinity of −6.4 kcal/mol and −6.3 kcal/ mol, respectively. Conclusion: It could be concluded that the essential oils potential sources of antibacterial, antioxidant activities, and molecular docking of bioactive components. The results of this study provide partial scientific support for the traditional application of essential oils to cure diarrhea and also major bioactive compounds responsible for important biological activities.

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