scholarly journals Detection of Biological Activity of Curcuma longa Methanol Extract and Its Antibacterial effect on Staphylococcus aureus

2021 ◽  
Vol 5 (2) ◽  
pp. 56-62
Author(s):  
Covenant Ogbonna ◽  
Erkay Özgör
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Methanol Extract ◽  
Curcuma Longa ◽  
Agar Plate ◽  
Negative Control ◽  
Penicillin G ◽  
Chemical Components ◽  
Bacterial Strains ◽  
Ic50 Values

Abstract Plants are known to possess enormous profound benefits, which if well incorporated in daily living have the potential to influence our health in ways unexpected. Significant progress has been made concerning the anti-oxidative and anti-cancerous effect of bioactive components in food products, worthy of note in the curcuminoids derived from turmeric (Curcuma longa) rhizomes. Therefore, to this end, this research aims to describe succinctly the phytocomponents, antioxidant activity and anti-microbial activity of turmeric methanol extract. The zones of inhibition were measured after plating on a Mueller Hinton agar plate and compared to the standards penicillin G and chloramphenicol. Chloramphenicol used as a positive control was found to be most effective on Staphylococcus aureus while Curcuma longa methanol extract and penicillin G were found to have the same microbial inhibition due to the negative control. Compared to the IC50 values of Ascorbic acid which was 5 mg/ ml, Curcuma longa methanol extract had a lower IC50 value is 3.2 mg/ml which according to previous literature is a good one given that lower IC50 values indicate great antioxidant activity. It was gathered by the observation that the qualitative extraction of Curcuma longa using methanol shows the presence of varying chemical components such as AR-tumerone, arachinsaeure, alpha-tumerone amongst others. Research data suggest that ar-turmerone has the potential to promote the positive and negative proliferation of tumor cell lines. As a result, Curcuma longa has high potential to be developed into an antibiotic against Methicillin-resistant Staphylococcus aureus and other clinically important bacterial strains in future.

scholarly journals Phytochemicals, Antimicrobials and Antioxidants Studies of the Stem Bark Extract from Calophyllum ferrugineum

2020 ◽  
Vol 17 (2) ◽  
pp. 1
Author(s):  
Izzah Afifah Noh ◽  
Vivien Yi Mian Jong
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Biological Activities ◽  
Stem Bark ◽  
Extraction Process ◽  
Bark Extract ◽  
Chemical Components ◽  
Isolation And Purification ◽  
Antioxidant Agents ◽  
Ic50 Values

Traditionally, Calophyllum genus was used for swollen gums, arthritis, diarrhea, chronic abscess, skin infections, and lesions treatment. C. ferrugineum from Sarawak has least studied about their phytochemical constituents and biological activities. The objectives are to isolate and characterize the chemical components from C. ferrugineum and to determine their antimicrobial and antioxidant activity. The plant stem barks were collected from National Park in Sarawak and underwent extraction process. The extracts underwent isolation and purification processes by using several chromatographic methods. Structural elucidation was achieved by using infrared, MS and NMR spectra. The isolation process from the species has afforded 5 known compounds;  isocalanone (1) and 1-hydroxy-7-methoxy-9H-xanthen-9-one (2), lupeol (3), friedelin (4) and diethylene glycol dibenzoate (5). The chloroform and methanol extract showed strong inhibitions against S. aureus, B. subtilis, P. aeruginosa and E. coli with the MIC and MBC values ranging from 225 and 112.5 µg/mL. Isocalanone (1) and the methanol extract of C. ferrugineum showed potent antioxidant activity with the IC50 values of 28 ± 5.23 and 35 ± 3.69 µg/mL compared to ascorbic acid, respectively. The results obtained from this study emphasized the potential of the species as antimicrobial and antioxidant agents.

scholarly journals STUDY ON CHEMICAL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF ALPINIA KWANGSIENSIS T. L. WU AND S. J. CHEN COLLECTED IN VIETNAM

2018 ◽  
Vol 56 (4A) ◽  
pp. 273
Author(s):  
Tran Thi Minh
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Spectroscopic Data ◽  
Methanol Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Ethyl Acetate Fraction ◽  
Ic50 Values

Three compounds named ester methyl-trans-p-coumarate (1), 7-hydroxy-6-methoxycoumarin (2), and (+)-gallocatechin (3) have been isolated from the ethyl acetate fraction of methanol extract of Alpinia kwangsiensis roots collected in Thai Nguyen province. Their structures were elucidated on the basis of spectroscopic data and by comparison with their spectral data reported in literature. This is the first isolation of three compounds from this species. The ethyl acetate fraction was found to be active against bacterias as Staphylococcus aureus and Bacillus subtilis with the IC50 values ranging at 74.65 mg/ml and 80.54 mg/ml, respectively. This fraction also showed antioxidant activity through DPPH test with the EC50 value 87.98 mg/ml.

scholarly journals Antioxidant, antimicrobial and anti-inflammatory activities development of methanol extract of Cyclamen africanum B. et R., growth in Jijel - Algeria

2020 ◽  
Vol 10 (1-s) ◽  
pp. 130-134
Author(s):  
Gaamoune Sofiane ◽  
Nouioua Wafa
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Methanol Extract ◽  
Reducing Power ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Anti Inflammatory

The genus Cyclamen L. (Primulaceae) is represented by 20 species, where inter alia Cyclamen africanum B. et R, is the only endemic species presented in Algeria. The study of their biological potentialities was evaluated through antioxidant, antimicrobial and anti-inflammatory activities. The antioxidant activity was evaluated by DPPH and the reducing power essay, the antimicrobial activity was tested with three bacterial strains and one yeast (Staphylococcus aureus ATCC25923, Klebsiella pneumonia ATCC700603, Bacillus subtilis ATCC6633 and Candida albicans ATCC1024) and anti-inflammatory activity with the Human Red Blood Cell (HRBC) membrane stabilization method. The results demonstrate a perfect antioxidant activity and an excellent anti- inflammatory power at very small concentrations. Keywords: Cyclamen; crude extract; reducing power; antimicrobial; HRBC.

Uşak İli’nden Toplanan Taxus Baccata (L) Türünün Metanol Ekstresinin Yaprak, Dal ve Meyvesinde Biyolojik Aktivitenin Belirlenmesi

2019 ◽  
Vol 7 (10) ◽  
pp. 1533
Author(s):  
Yasemin Karafakıoglu
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Maximum Amount ◽  
Chemical Components ◽  
Taxus Baccata ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Future Studies ◽  
Different Parts

Taxus species in different parts of the world prevent the formation of hepatoprotective, anticoagulant, antiulcerogenic, anti-coagulant, antifungal and tumour cells. In this study, biological activity studies were performed on the leaves, branches and fruits of the methanol extract of Taxus baccata L. species collected from Sivaslı district of Uşak province. The methanol extract of the T.baccata species, the total amount of phenolic content of the sample was found to be 14.76 mg GAEQ/1g dry sample in the highest leaves. In T.baccata methanol extract, the maximum amount of flavonoid content was 0.468±mg QE/g dry sample. Antioxidant activity value as DPPH radical; % inhibition value was found as 93.21%. Based on the results; It can be concluded that the leaves of T.baccata have higher antioxidant activity than fruits and branches. Future studies need to explore the chemical components contained in the T.baccata species to determine and investigate in further detail.

scholarly journals Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Irfan Zamzani ◽  
Nita Triadisti
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Oxidation Process ◽  
Antioxidant Activities ◽  
Alternative Source ◽  
Dpph Method ◽  
Indigenous Plant ◽  
Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

Plasmid profile analysis and evaluation of antibiotic susceptibility of Staphylococcus aureus strains isolated from table chicken eggs

2013 ◽  
Vol 16 (2) ◽  
pp. 307-312 ◽  
Author(s):  
E. Pyzik ◽  
A. Marek
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Profile Analysis ◽  
Plasmid Profile ◽  
Penicillin G ◽  
Bacterial Strains ◽  
Phenotypic Properties ◽  
Molecular Weights ◽  
Antibiotic Susceptibility Patterns ◽  
Chicken Eggs

AbstractThe aim of this study was to isolate and characterize Staphylococcus aureus bacteria present on the shell surfaces and in the contents of chicken eggs, taking into account their phenotypic properties, antibiotic susceptibility patterns, and the presence of plasmid DNA. The study included 90 table chicken eggs from laying farms situated in the vicinity of Lublin. A total of 105 bacterial strains identified as Staphylococcus were isolated from the material, of which 18 (17.14%) were of the species Staphylococcus aureus. All 18 S. aureus strains were found to be resistant to at least one of the antibiotics tested, while some (55.55%) showed resistance to five or more of the 17 therapeutic agents. The greatest number of strains showed resistance to erythromycin (66.66%), tetracycline (66.66%), oxytetracycline (61.11%), penicillin G (50%), and amoxicillin (44.44%). The plasmid profile analysis of the S. aureus strains made it possible to evaluate the dependence between antibiotic susceptibility and the presence of plasmids in particular isolates. The results showed that plasmids in various quantities and of varying molecular weights were isolated from 17 of the strains. Most often isolated were small plasmids, of 5.6 kb - from 11 of the S. aureus strains (61.11%), 2.5 kb - from 9 strains (50%), 4.1 kb - from 8 (44.44%), and 4.6 kb - from 7 (38.88%) of the strains.

scholarly journals The Measurement of Antioxidant Activity of Velvet Beans (Mucuna pruriens) and Velvet Beans (Mucuna pruriens) in Coffee Preparations

Biomedika ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 117-122
Author(s):  
Indah Tri Susilowati ◽  
Purwati Purwati
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Mucuna Pruriens ◽  
Methanol Extracts ◽  
Activity Test ◽  
Antioxidant Content ◽  
Ic50 Values

Antioxidant activity tests of methanol extracts of velvet beans (Mucuna pruriens) and velvet beans (Mucuna pruriens) in coffee preparations have been previously carried out by several researchers. This present study aims to examine the phytochemical and antioxidant activities of methanol extracts of Mucuna pruriens and Mucuna pruriens in coffee preparations. The methanol extract was tested for the phytochemical and antioxidant activities by applying DPPH (1,1-Dhiphenyl-2-picrylhydrazil) method. The results of the phytochemical test showed that the methanol extracts of the Mucuna pruriens and Mucuna pruriens in coffee preparations contained secondary metabolites of flavonoids, alkaloids, saponins, terpenoids, and tannins. The outcomes of the antioxidant activity test revealed that the methanol extracts of the Mucuna pruriens and Mucuna pruriens in coffee preparations had antioxidant activities, as indicated by the IC50 values of 42.09 ppm and 37.23 ppm. It was also revealed that the antioxidant content of Mucuna pruriens in coffee preparations was stronger than the content in the velvet beans alone.

scholarly journals Aktivitas Antioksidan Ekstrak Metanol Daun Alpukat Menggunakan Metode DPPH

2021 ◽  
Vol 11 (2) ◽  
pp. 104-110
Author(s):  
Alfin Surya ◽  
Zaiyar Nazir ◽  
Anggun Syazulfa
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radicals ◽  
Methanol Extract ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values

Free radicals are molecules that lose electrons in their outer orbitals so that the number of electrons becomes odd and unstable. Free radicals can damage various macromolecule cells including proteins, carbohydrates, fats. Antioxidants are compounds that can donate one or more electrons to free radicals. Avocado leaves contain high bioactive components that can be used as a source of natural antioxidants. Avocado leaves that have been dried, mashed and then shifted with methanol. The extracts were tested by phytochemical screening, including flavonpoid, phenolic, tannin and antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhidrazyl) method. This method is based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) against free radicals which causes a change from purple to yellow 2,2-diphenyl-1-picrylhydrazyl) (DPPH). From the research, the IC50 values ​​in avocado leaves were 118.8056 µg / mL and 7,276 µg / mL on ascorbic acid as a positive control. From the IC50 value obtained, it is known that the methanol extract of avocado leaves has strong antioxidant activity to fight free radicals.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANG KUNYIT (Curcuma longa L.) TERHADAP Staphylococcus aureus DAN Pseudomonas aeruginosa

2019 ◽  
Vol 4 (1) ◽  
pp. 278-287
Author(s):  
Lia Fikayuniar ◽  
Neni Sri Gunarti ◽  
Mellya Apriliani
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Curcuma Longa ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Paper Disk

ABSTRAK Kunyit (Curcuma longa L.) merupakan salah satu jenis tanaman obat yang termasuk dalam keluarga Zingiberaceae. Senyawa aktif yang terkandung dalam rimpang kunyit (Curcuma longa L.) mampu bekerja sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak etanol rimpang kunyit (Curcuma longa L.) terhadap Staphylococcus aureus dan Pseudomonas aeruginosa. Ekstraksi dilakukan dengan cara refluks menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri dilakukan menggunakan metode difusi paper disk dengan masing-masing konsentrasi ekstrak 10%, 20%, 30%, 40% b/v. Kontrol positif yang digunakan adalah Ciprofloxacin sedangkan kontrol negatif yang digunakan adalah DMSO. Hasil skrining fitokimia ekstrak etanol rimpang kunyit mengandung alkaloid, flavonoid, fenol, tanin dan terpenoid. Berdasarkan hasil penelitian, ekstrak etanol rimpang kunyit dapat menghambat bakteri Staphylococcus aureus dan Pseudomonas aeruginosa pada konsentrasi 10%, 20%, 30%, 40% dan konsentrasi 40% merupakan konsentrasi yang memberikan diameter zona hambat terbesar terhadap kedua bakteri uji yaitu 8,63 mm dan 7,8 mm. Kata Kunci : aktivitas antibakteri, Curcuma longa L., Staphylococcus aureus,    ABSTRACT Turmeric (Curcuma longa L.) is one type of medicinal plant that belongs to the Zingiberaceae family. The active compounds contained in the Curcuma longa L. rhizome can work as antibacterial. This study aims to determine the antibacterial activity of the ethanol extract of Curcuma longa L. Antibacterial activity testing was carried out using the paper disk diffusion method with each extract concentration of 10%, 20%, 30%, 40%. The positive control used was Ciprofloxacin while the negative control used was DMSO. The results of phytochemical screening of the ethanol extract of Curcuma longa L. rhizome contain alkaloids, flavonoids, phenols, tannins and terpenoids. Based on the results of the study, the ethanol extract of turmeric rhizome can inhibit Staphylococcus aureus and Pseudomonas aeruginosa bacteria at concentrations of 10%, 20%, 30%, 40% and 40% concentrations which give the largest inhibition zone diameter of the two test bacteria which is 8.63 mm and 7.8 mm. Keywords: antibacterial activity, Curcuma longa L., Staphylococcus aureus,  

scholarly journals Evaluation of antibacterial and antioxidant activities of three types of benzoin resin

2018 ◽  
Vol 9 (4) ◽  
pp. 408-411
Author(s):  
Zineb Hacini ◽  
Fatima Khedja ◽  
Ibrahim Habib ◽  
Zaouia Kendour ◽  
Zineb Debba
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antioxidant Activities ◽  
Ic50 Values ◽  
Therapeutic Properties ◽  
Resin Extract ◽  
And Staphylococcus Aureus

The benzoin resin is used extensively in traditional medicine for its many reported therapeutic properties. The essential oils of three different types of benzoin resin were extracted using the traditional method in this study. The yield of essential oils of the white, red and gray types of resin was 1.01, 0.92 and 0.54%, respectively. The obtained extracts were tested against two types of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. The tests showed that essential oil of gray type resin is effective against both Escherichia coli (14 mm) and Staphylococcus aureus (11 mm). The antioxidant activity has been also evaluated to compare the efficiency of different type of resin with DPPH· assay. In the DPPH· system, the antioxidant activity of the red resin extract (0.01 μg/mL) was superior to that of the white (27.32 μg/mL) and gray (42.90 μg/mL) extracts, with IC50 values, respectively.

Sign in / Sign up

Export Citation Format

Share Document