Mosquito larvicidal efficacy of phenolic acids of seaweed Chaetomorpha antennina (Bory) Kuetz. against Aedes aegypti

Biologia ◽  
2012 ◽  
Vol 67 (1) ◽  
Author(s):  
Subramanium Vimaladevi ◽  
Ayyavu Mahesh ◽  
Balaji Dhayanithi ◽  
Nattarayan Karthikeyan
Keyword(s):  
Aedes Aegypti ◽  
Phenolic Acids ◽  
Phenolic Acid ◽  
Wide Spectrum ◽  
Larval Mortality ◽  
Inhibitory Effect ◽  
The Third ◽  
Third Instar Larvae

AbstractMosquito larvicidal and repellent activities of phenolic acids of Chaetomorpha antennina (Bory) Kuetz. against the third instar larvae of Aedes aegypti were investigated. The larval mortality was observed after 24 h exposure. Results of mosquito larvicidal tests revealed that insoluble bound phenolic acids and soluble conjugated phenolic acid fractions of C. antennina had an excellent inhibitory effect against A. aegypti and its LC50 values were 23.4 and 44.6 μg ml−1, respectively. The repellency assay of insoluble bound phenolic acids and soluble conjugated phenolic acid fractions of C. antennina, at 10 μg cm−2 concentration gave 100% protection up to 120 min. The results indicate that phenolic acids of C. antennina have a wide spectrum of larvicidal and repellent activities against Aedes aegypti.

scholarly journals Larvicidal Activity of Ethyl Acetate Extract of Derris elliptica Root against the Third-instar Larvae of Cypermethrin-Resistant Aedes aegypti Offspring

2021 ◽  
Author(s):  
Sayono Sayono ◽  
Risyandi Anwar ◽  
Didik Sumanto
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Ethyl Acetate Extract ◽  
Larval Mortality ◽  
Laboratory Strain ◽  
The Third ◽  
Resistant Strains ◽  
Four Levels ◽  
Third Instar Larvae

Background: Derris elliptica extracts have a high larvicidal potential against the laboratory strain of Aedes aegypti larvae, but the effect on offspring larvae of pyrethroid-resistant strains of the species is lack understood. This study aimed to determine the larvicidal activity of the ethyl acetate extract of tuba root against the third-instar larvae of the Cypermethrin-resistant Ae. aegypti offspring. Methods: The experimental study occupied four levels of ethyl acetate extract of D. elliptica namely 10, 25, 50, and 100 ppm, and each level was four times replicated. As many as twenty of healthy third-instar larvae, offspring of Cypermethrin-resistant Ae. aegypti were subjected to each experiment group. Larval mortality rate and lethal concentration 50% subject (LC50) were calculated after 24 and 48 hours of exposure time. Results: Mortality of larvae increased directly proportional to the increase of extract concentration. Larval mortality rates after 24 and 48 hours of exposure were 40–67.5% and 62.5–97.5%, and LC50 were 34.945 and 6.461ppm, respectively. Conclusion: The ethyl acetate extract of D. elliptica has the high effectiveness larvicidal potential against the thirdinstar larvae, offspring of the Cypermethrin-resistant Ae. aegypti. Isolation of the specific compound is necessarily done to obtain the active ingredient for larvicide formulation.

scholarly journals EFFECT OF Gliricidia sepium LEAVES EXTRACTS ON Aedes aegypti: LARVICIDAL ACTIVITY

2015 ◽  
Vol 7 ◽  
pp. 26 ◽  
Author(s):  
Krishnaveni K.V. ◽  
ThaiyalNayaki R. ◽  
Balasubramanian, G M*.
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Larval Mortality ◽  
Ethanolic Extract ◽  
Gliricidia Sepium ◽  
Inhibitory Effect ◽  
High Rate ◽  
Phytochemical Analysis ◽  
Significant Difference ◽  
Health Significance

<p>Mosquitoes are the single most important group of insects in terms of public health significance and causing diseases. They are the vectors for the transmission of many viral pathogens and pose serious threat to human health. Chemical insecticides are widely used but they are non-selective and harmful to beneficial organisms. In the present study <em>G. Sepium</em> leaves extracts were used to analyse its effect against <em>Aedes aegypti</em>, to compare the mortality rate while using different solvent extracts of the leaf, to identify the solvent extract which give high rate of larval mortality and to characterise the compounds present in the corresponding leaf extract using phytochemical analysis. The ethanolic extract of <em>G. sepium</em> leaves constitute flavanoids, steroids, glycoside, carbohydrate and saponins compound. Thus it was found to have an inhibitory effect on the growth of larvae than other solvent extracts. In the statistical analysis highest significant difference was observed between 1.5g/l and 2.0g/l concentration of ethanolic extract and other extracts. However no significant difference was observed in other concentrations. The larvicidal activity of the plant extract may be attributed to the presence of active compounds like terpinoids, saponins and steroids.<strong></strong></p>

scholarly journals Pemanfaatan Jeruk Purut (Citrus Hystrix) Sebagai Biolarvasida

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Titik Lestari
Keyword(s):  
Essential Oil ◽  
Literature Review ◽  
Aedes Aegypti ◽  
Research Methods ◽  
Chemical Compound ◽  
Mosquito Vector ◽  
The Third ◽  
Chemical Content ◽  
Lethal Time ◽  
Third Instar Larvae

Absctract: Citrus hystrix, Biolarvicides. Insectisides and pestisides were used as larvicides to control mosquito vector Aedes aegypti particularly. Some research suggested there had been a larvae resistance to pesticides. Biolarvicides with basic ingredients plant was another alternative that can be used. Lime (Citrus hystrix) was one of plants that often used as biolarvicides, both lits fruit and leaves. This paper aimed to know about chemical compound in lime and its potential as biolarvicidal. Research methods was literature review from several research journals. The results showed that the presence of coumpounds were essential oil, flavonoids, saponins, terpeesn, limonoids, citronellal, linalool, citronellol, acetate citronelil, kariofilin and geraniol. LC50 of lime leaves as biolarvicides againts the third instar larvae of Aedes aegypti was 279.882 ppm, biolarvicides oxicity values (LC90) was 4000 ppm with the lethal time (LT90) was 12.67 hours. Based on results of this study concluded that the presence of the chemical content of Citrus hystrix plant potential as biolarvicides.

scholarly journals New Purinyl-Steroid and Other Constituents from the Marine Fungus Penicillium brefeldianum ABC190807: Larvicidal Activities against Aedes aegypti

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Zong-Min Hou ◽  
Sen-Quan Yu ◽  
Min Tao ◽  
Cai-Bei Xia ◽  
Yu-Lian Xia ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
2D Nmr ◽  
Marine Fungus ◽  
Mangrove Sediment ◽  
Health Issues ◽  
Etoac Extract ◽  
The Third ◽  
Larvicidal Activities ◽  
Third Instar Larvae

Mosquitoes historically threatened human health; the major mosquito-related global health issues include malaria, dengue fever, yellow fever, and Zika, as well as several other vector-borne outbreaks. Here, the EtOAc extract of the mangrove sediment fungus Penicillium brefeldianum ABC190807 exerted larvicidal activities against the third instar larvae of Aedes aegypti with an LC50 of 0.089 mg/mL. One new purinyl-steroid (ergosta-4,6,8(14),22-tetraen-3-(6-amino-9H-purin-9-yl) (1)), along with six (2–7) known compounds, were isolated from the EtOAc extract of Penicillium brefeldianum ABC190807. Structures of the compounds were elucidated via 1D/2D NMR and HR-ESI-MS data. Respective spectral data were compared with those of known compounds. Among all compounds whose larvicidal activity against the third instar larvae of Aedes aegypti was evaluated, compounds 2 and 7 showed larvicidal activity with respective LC50 values of 0.452 and 0.337 mg/mL.

scholarly journals Momordica charantia L. extracts against Aedes aegypti larvae

2022 ◽  
Vol 82 ◽  
Author(s):  
L. M. P. Mituiassu ◽  
M. T. Serdeiro ◽  
R. R. B. T. Vieira ◽  
L. S. Oliveira ◽  
M. Maleck
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Methanol Extract ◽  
Larval Mortality ◽  
High Toxicity ◽  
The Third ◽  
Low Toxicity ◽  
Aegypti Population ◽  
Ethyl Acetate Extracts

Abstract Mormodica charantia (Curcubitaceae) is a plant with great medicinal potential, also used as an alternative of mosquitoes control as demonstrated by previous studies. We evaluated the larvicidal activity of crude extracts of ethyl acetate, methanol and hexane from flowers and fruits of M. charantia against Aedes aegypti (Culicidae). Flowers and fruits were macerated in methanol, ethyl acetate and hexane. Bioassays were performed with application of the extracts at final concentrations of 1 - 200 µg/mL in the middle of the third instar larvae of A. aegypti (L3). The results showed high toxicity to ethyl acetate extracts from flowers and fruits at concentrations of 200 µg/mL and 100 µg/mL, with 97% and 87% of larvae mortality (L3), respectively. Hexane extract demonstrated low toxicity, while methanol extract exhibited 78% larval mortality. The data suggested that the ethyl acetate extracts of flowers and fruits of M. charantia can effectively contribute to larvicidal activity. In addition, purification of M. charantia extracts may lead to a promising larvicidal activity to control the A. aegypti population.

scholarly journals Expression in Escherichia coli of Native and Chimeric Phenolic Acid Decarboxylases with Modified Enzymatic Activities and Method for Screening Recombinant E. coli Strains Expressing These Enzymes

2001 ◽  
Vol 67 (3) ◽  
pp. 1063-1069 ◽  
Author(s):  
Lise Barthelmebs ◽  
Charles Diviès ◽  
Jean-François Cavin
Keyword(s):  
Escherichia Coli ◽  
Phenolic Acids ◽  
Phenolic Acid ◽  
Inhibitory Effect ◽  
Property A ◽  
E Coli ◽  
Phenol Derivatives ◽  
Activity Form ◽  
Vinyl Phenol ◽  
Carboxy Terminal

ABSTRACT Four bacterial phenolic acid decarboxylases (PAD) fromLactobacillus plantarum, Pediococcus pentosaceus, Bacillus subtilis, and Bacillus pumilus were expressed in Escherichia coli, and their activities on p-coumaric, ferulic, and caffeic acids were compared. Although these four enzymes displayed 61% amino acid sequence identity, they exhibit different activities for ferulic and caffeic acid metabolism. To elucidate the domain(s) that determines these differences, chimeric PAD proteins were constructed and expressed in E. coli by exchanging their individual carboxy-terminal portions. Analysis of the chimeric enzyme activities suggests that the C-terminal region may be involved in determining PAD substrate specificity and catalytic capacity. In order to test phenolic acid toxicity, the levels of growth of recombinant E. colidisplaying and not displaying PAD activity were compared on medium supplemented with different concentrations of phenolic acids and with differing pHs. Though these acids already have a slight inhibitory effect on E. coli, vinyl phenol derivatives, created during decarboxylation of phenolic acids, were much more inhibitory to theE. coli control strain. To take advantage of this property, a solid medium with the appropriate pH and phenolic acid concentration was developed; in this medium the recombinant E. colistrains expressing PAD activity form colonies approximately five times smaller than those formed by strains devoid of PAD activity.

Larvicidal Activity of Essential Oils Against Aedes aegypti (Diptera: Culicidae)

2020 ◽  
Vol 26 (33) ◽  
pp. 4092-4111
Author(s):  
Mikael A. de Souza ◽  
Larissa da Silva ◽  
Maria A. C. dos Santos ◽  
Márcia J. F. Macêdo ◽  
Luiz J. Lacerda-Neto ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Essential Oils ◽  
Larval Mortality ◽  
Analytical Techniques ◽  
Extraction Methods ◽  
Control Programs ◽  
Plant Parts ◽  
Natural Insecticides ◽  
Methods Analytical ◽  
Meta Analyses

The Aedes aegypti is responsible for the transmission of arboviruses, which compromise public health. In the search for synthetic product alternatives, essential oils (OEs) have been highlighted by many researchers as natural insecticides. This systematic review (SR) was performed according to PRISMA guidelines (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) and its objective was to evaluate studies addressing OEs with larvicidal properties against Ae. aegypti, through electronic database searches (Pubmed, Science Direct and Scielo), covering an overview of the plant sources OEs, which plant parts were used, the extraction methods, analytical techniques, major and/or secondary constituents with greater percentages, as well as the LC50s responsible for larval mortality. Following study analysis, plants distributed across 32 families, 90 genera and 175 species were identified. The Lamiaceae, Myrtaceae, Piperaceae, Asteraceae, Rutaceae, Euphorbiaceae and Lauraceae families obtained the highest number of species with toxic properties against larvae from this vector. Practically all plant parts were found to be used for OE extraction. Hydrodistillation and steam distillation were the main extraction methods identified, with GC-MS/GC-FID representing the main analytical techniques used to reveal their chemical composition, especially of terpene compounds. In this context, OEs are promising alternatives for the investigation of natural, ecologically correct and biodegradable insecticides with the potential to be used in Ae. aegypti control programs.

Phenolic Acids Exert Anticholinesterase and Cognition-Improving Effects

2018 ◽  
Vol 15 (6) ◽  
pp. 531-543 ◽  
Author(s):  
Dominik Szwajgier ◽  
Ewa Baranowska-Wojcik ◽  
Kamila Borowiec
Keyword(s):  
Phenolic Acids ◽  
Ex Vivo ◽  
Amyloid Β ◽  
Inhibitory Effect ◽  
In Vivo Studies ◽  
Amyloid Β Peptide ◽  
Beneficial Effects ◽  
Aβ Fibrils

Numerous authors have provided evidence regarding the beneficial effects of phenolic acids and their derivatives against Alzheimer's disease (AD). In this review, the role of phenolic acids as inhibitors of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) is discussed, including the structure-activity relationship. In addition, the inhibitory effect of phenolic acids on the formation of amyloid β-peptide (Aβ) fibrils is presented. We also cover the in vitro, ex vivo, and in vivo studies concerning the prevention and treatment of the cognitive enhancement.

Effect of guaianolides in the meiosis reinitiation of amphibian oocytes

Zygote ◽  
2016 ◽  
Vol 25 (1) ◽  
pp. 10-16 ◽  
Author(s):  
J. Zapata-Martínez ◽  
G. Sánchez-Toranzo ◽  
F. Chaín ◽  
C.A.N. Catalán ◽  
M.I. Bühler
Keyword(s):  
Biological Activities ◽  
Wide Spectrum ◽  
Sesquiterpene Lactones ◽  
Inhibitory Effect ◽  
Biological Molecules ◽  
Plant Metabolites ◽  
Major Mechanism ◽  
Bufo Arenarum ◽  
Asteraceae Family

SummarySesquiterpene lactones (STLs) are a large and structurally diverse group of plant metabolites generally found in the Asteraceae family. STLs exhibit a wide spectrum of biological activities and it is generally accepted that their major mechanism of action is the alkylation of the thiol groups of biological molecules. The guaianolides is one of various groups of STLs. Anti-tumour and anti-migraine effects, an allergenic agent, an inhibitor of smooth muscle cells and of meristematic cell proliferation are only a few of the most commonly reported activities of STLs. In amphibians, fully grown ovarian oocytes are arrested at the beginning of meiosis I. Under stimulus with progesterone, this meiotic arrest is released and meiosis progresses to metaphase II, a process known as oocyte maturation. There are previous records of the inhibitory effect of dehydroleucodin (DhL), a guaianolide lactone, on the progression of meiosis. It has been also shown that DhL and its 11,13-dihydroderivative (2H-DhL; a mixture of epimers at C-11) act as blockers of the resumption of meiosis in fully grown ovarian oocytes from the amphibian Rhinella arenarum (formerly classified as Bufo arenarum). The aim of this study was to analyze the effect of four closely related guaianolides, i.e., DhL, achillin, desacetoxymatricarin and estafietin as possible inhibitors of meiosis in oocytes of amphibians in vitro and discuss some structure–activity relationships. It was found that the inhibitory effect on meiosis resumption is greater when the lactone has two potentially reactive centres, either a α,β–α′,β′-diunsaturated cyclopentanone moiety or an epoxide group plus an exo-methylene-γ-lactone function.

Sign in / Sign up

Export Citation Format

Share Document