scholarly journals EFFECT OF Gliricidia sepium LEAVES EXTRACTS ON Aedes aegypti: LARVICIDAL ACTIVITY

2015 ◽  
Vol 7 ◽  
pp. 26 ◽  
Author(s):  
Krishnaveni K.V. ◽  
ThaiyalNayaki R. ◽  
Balasubramanian, G M*.
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Larval Mortality ◽  
Ethanolic Extract ◽  
Gliricidia Sepium ◽  
Inhibitory Effect ◽  
High Rate ◽  
Phytochemical Analysis ◽  
Significant Difference ◽  
Health Significance

<p>Mosquitoes are the single most important group of insects in terms of public health significance and causing diseases. They are the vectors for the transmission of many viral pathogens and pose serious threat to human health. Chemical insecticides are widely used but they are non-selective and harmful to beneficial organisms. In the present study <em>G. Sepium</em> leaves extracts were used to analyse its effect against <em>Aedes aegypti</em>, to compare the mortality rate while using different solvent extracts of the leaf, to identify the solvent extract which give high rate of larval mortality and to characterise the compounds present in the corresponding leaf extract using phytochemical analysis. The ethanolic extract of <em>G. sepium</em> leaves constitute flavanoids, steroids, glycoside, carbohydrate and saponins compound. Thus it was found to have an inhibitory effect on the growth of larvae than other solvent extracts. In the statistical analysis highest significant difference was observed between 1.5g/l and 2.0g/l concentration of ethanolic extract and other extracts. However no significant difference was observed in other concentrations. The larvicidal activity of the plant extract may be attributed to the presence of active compounds like terpinoids, saponins and steroids.<strong></strong></p>

scholarly journals Larvicidal Effectiveness of Azolla pinnata against Aedes aegypti (Diptera: Culicidae) with Its Effects on Larval Morphology and Visualization of Behavioural Response

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Nor Shaida Husna Zulkrnin ◽  
Nurul Nadiah Rozhan ◽  
Nur Amanina Zulkfili ◽  
Nik Raihan Nik Yusoff ◽  
Mohd Sukhairi Mat Rasat ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Larval Mortality ◽  
Control Program ◽  
Test Method ◽  
World Health ◽  
Azolla Pinnata ◽  
Agricultural Field ◽  
Aquatic Fern ◽  
Significant Difference

Dengue is vector-borne diseases with 390 million infections per year extending over 120 countries of the world. Aedes aegypti (L.) (Diptera: Culicidae) is a primary vector for dengue viral infections for humans. Current focus on application of natural product against mosquito vectors has been the main priority for research due to its eco-safety. The extensive use of chemical insecticides has led to severe health problems, environmental pollution, toxic hazards to human and nontarget species, and development of insecticide resistance on mosquitoes. Azolla pinnata is an aquatic fern and predominantly used as feed in poultry industry and as fertilizer in agricultural field for enhancing the fertility of rice paddy soil. The present study was conducted to explore the larvicidal efficacy of A. pinnata using fresh and powdered form against late third-stage larvae (6 days, 5 mm in larvae body length) of Ae. aegypti (L.) (Diptera: Culicidae). The larvicidal bioassays were performed using World Health Organization standard larval susceptibility test method for different concentration for powdered and fresh A. pinnata. Powdered A. pinnata concentration used during larvicidal bioassay ranges from 500ppm to 2000ppm; meanwhile, fresh A. pinnata ranges from 500ppm to 9,000,000 ppm. The highest mortality was at 1853 ppm for powdered A. pinnata compared with fresh A. pinnata at 2,521,535 ppm, while the LC50 for both powdered and fresh A. pinnata recorded at 1262 ppm and 1853 ppm, respectively. Finally, the analysis of variance (ANOVA) showed significant difference on Ae. aegypti larval mortality (F=30.439, df=1, p≤0.001) and concentration (F=20.002, df=1, p≤0.001) compared to powdered and fresh A. pinnata at 24-hour bioassay test. In conclusion, the powdered A. pinnata serves as a good larvicidal agent against Ae. aegypti (L.) (Diptera: Culicidae) and this study provided information on the lethal concentration that may have potential for a more eco-friendly Aedes mosquito control program.

scholarly journals Larvicidal Effect of Vinca Fruit Extract (Vinca rosea) Against Aedes aegypti Larvae and Secondary Metabolites Profile by Thin Layer Chromatography

2014 ◽  
Vol 3 (1) ◽  
pp. 31
Author(s):  
Rahmawati Ekaputri ◽  
Sudarsono Sudarsono ◽  
Budi Mulyaningsih
Keyword(s):  
Aedes Aegypti ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Larvicidal Activity ◽  
Larval Mortality ◽  
Probit Analysis ◽  
Mosquito Vector ◽  
Fruit Extract ◽  
Vinca Rosea ◽  
Layer Chromatography

<p>Background: <em>Vinca rosea</em> is known contain alkaloids, it was usually used to treat various diseases. Alkaloids from Vinca leaves are also already known have larvicidal activity. Based on this toxicological activity, the fruit of <em>Vinca rosea</em> was selected to investigation its larvicidal activity against the 3<sup>rd</sup> instar larvae of the mosquito vector of dengue haemorrhagic fever (DHF) <em>Aedes aegypti.</em>  Five concentrations of Vinca fruit extract were tested against the 3<sup>rd</sup> instar Aedes aegypti larvae. The different larval mortality percentages were recorded after 24 hours. Lethal concentration (LC<sub>50</sub> anf LC<sub>90</sub>) of Vinca fruit extract were calculated using Probit analysis. Phytochemical compounds  of ethanolic extract also investigated using Thin layer Chromatography (TLC). LC<sub>50</sub> and LC<sub>90</sub> values of fruit extract were 2.987 mg/ml and 32.861 mg/ml. Alkaloids were detected in extract.</p>

scholarly journals INHIBITORY EFFECT OF ETHANOLIC EXTRACT OF ERIOBOTRYA JAPONICA LEAVES PRE-INCUBATED WITH THEOPHYLLINE AND ASPIRIN ON ISOLATED GUINEA PIG TRACHEAL

2018 ◽  
Vol 11 (13) ◽  
pp. 46
Author(s):  
Marianne Marianne ◽  
Urip Harahap ◽  
Emil Salim ◽  
Dadang Irfan Husori ◽  
Fahrumsyah Jali Rambe ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Protective Effect ◽  
Contractile Response ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Eriobotrya Japonica ◽  
Relaxant Effect ◽  
Significant Difference

 Objectives: The objectives of the study were to examine the inhibitory effect of ethanol extract of Eriobotrya japonica leaves (EEEJL) pre-incubated with theophylline and aspirin on isolated guinea pig tracheal chains against acetylcholine (ACh)-induced contraction.Methods: The effect of EEEJL (1-8 mg/Ml) on the isolated tracheal strips was tested in vitro. Furthermore, the mechanism of relaxant effects of EEEJL was evaluated in the presence of theophylline and aspirin.Results: The contractile response evoked by Ach (1.25 × 10−3 M) was decreased by EEEJL (effective concentration50 = 1.36 mg/mL) and has no significant difference of relaxant effect to that of EEEJL pre-incubated with theophylline and aspirin (p>0.05).Conclusion: The EEEJL decreased the ACh-induced contraction through the inhibition of PDE and the protective effect on prostaglandin E2.

scholarly journals Larvicidal Activity of Native Plant Extracts From the Araripe National Forest on Aedes aegypti

2019 ◽  
Vol 11 (7) ◽  
pp. 105
Author(s):  
Francisco R. de Azevedo ◽  
Glauber C. Maciel ◽  
Gilberto B. Oliveira e Silva ◽  
Francisco de O. Mesquita ◽  
Antonio C. Leite Alves
Keyword(s):  
Aedes Aegypti ◽  
Native Plants ◽  
Larval Mortality ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
National Forest ◽  
Native Plant ◽  
Chemical Insecticide ◽  
Ethanolic Extracts ◽  
Different Doses

Aiming to verify the insecticidal potential of 16 native plants from the Araripe National Forest (ANFO) on L3 Aedes aegypti larvae in laboratory conditions, were researches performed in controlled conditions of temperature, air relative humidity and photophase, in a type B.O.D. climatized chamber, executed in period from August/2016 to May/2017. The adopted experimental design was completely randomized, represented by ethanolic extracts obtained from 16 native plants, besides the witness without application and with pyriproxyfen as chemical insecticide, conducted with four replicates in each plant extract. The application of the extracts was performed only once, and at 24, 48 and 72 hours, after the infestation, was determined the effects of the extracts on larval mortality. The extracts of Amargoso, oil&rsquo;tree, Lacre, Cajui, Louro smelling, Field Rosemary, Murici truthful, Janaguba and laranjinha provoke mortality above of 90% to the larvae of Aedes aegypti after three days of exposure, in the dose of 50 mL of the extracts. After 24 hours of exposure, the ethanolic extracts from barks and leaves of Field Rosemary and of laranjinha killed all the larvae. On the other hand, the extracts with higher larvicide potential were evaluated at different doses (i.e., 12.5, 25, and 50 mL/L) in a 9 &times; 3 factorial scheme, with four replicates. The ethanolic extract of the leaves of Louro smelling is the most efficient, because in any dosage it eliminates all the larvae of Aedes aegypti, thus demonstrating to be an excellent vegetable larvicide in the control of this vector after three days of exposure, whereas the bark extract of the oil&rsquo;tree is the least effective. The alkaloids and flavonoids are present in the leaves of the Louro smelling.

scholarly journals Effect of plant extracts of Salvia officinalis L. and Costus speciosus L. in growth of some bacteria species isolated from urinary tract infections in Samarra city

2020 ◽  
Vol 25 (1) ◽  
pp. 47
Author(s):  
Huda Ali Hadi ◽  
Rasheed H. Hasan
Keyword(s):  
Urinary Tract ◽  
Methanol Extract ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Salvia Officinalis ◽  
Alcoholic Extract ◽  
Costus Speciosus ◽  
Significant Difference ◽  
Salvia Officinalis L ◽  
Tract Infections

The present study aimed to investigate the inhibitory effect of alcoholic extract (methanol - ethanol) and flavonoids by diffusion  methods of wells of Salvia officinalis L. and Costus speciosus L. plants on Escherichia . coli and Proteus  spp. isolated from patients with urinary tract infection Samarra city, the samples were collected during the period from 15/7/2018 to 30/9/2018, It was found through the results that the extracts plant that used in this study showed various inhibitory effect against, The bacteria species study imitation , where on differ this effect by difference the concentration that used and type the plant extract , showing  E.coli significant difference at significant (p< 0.05) in inhibition diameter of alcoholic (methanol) extract for Salvia officinalis L. (10.61±7.5) at higher concentration used, while these bacteria showed on significant difference in the level of inhibition diameter of alcoholic (ethanolic) extract for Salvia officinalis L., whereas the flavonoids Netted significant difference of these plant in some of concentration that used comparied with the antibiotic that used in this study. Either regarding proteus spp. bacteria notted no significant difference of both the Costus speciosus L. and Salvia officinalis L. by using the alcoholic (ethanolic) extract, while the flavonoids extract showed no significant difference in Costus speciosus L., whereas showed high significant difference in flavonoids extract of Salvia officinalis  L., due to the fact that the flavonoids extract is one of the active substance that done laboratory extraction for both the plants where that higher inhibitory effect of microbial growth from the alcoholic (ethanolic – methanolic) extract of plants .    http://dx.doi.org/10.25130/tjps.25.2020.009

scholarly journals Larvicidal activity of Vietnamese Solanum nigrum on mosquitoes Aedes aegypti and Aedes albopictus (Diptera: Culicidae)

2020 ◽  
Vol 52 (1) ◽  
Author(s):  
T.D.H. Dinh ◽  
Q.T. Le ◽  
T.D. Nguyen ◽  
T.Q.T. Nguyen ◽  
A.S. Ho ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
Plant Extract ◽  
Ethanolic Extract ◽  
Laboratory Strain ◽  
Hemorrhagic Fever ◽  
Solanum Nigrum ◽  
Hot Water ◽  
Control Effort

A Vietnamese domestic plant namely Solanum nigrum (S. nigrum) was subjected to test for larvicidal activity on two majors Dengue hemorrhagic fever (DHF) vectors Aedes aegypti (Ae. aegypti) and Aedes albopictus (Ae. albopictus). The plant was processed to get infusions in hot water or extracted in ethanol. Laboratory and field larval strains of two Aedes species were exposed to the infusions and extract at increasing concentrations for one hour and followed-up intensively for up to 72 hours. The obtained results of bioassay showed larvicidal effects of extract on all mosquito strains. The effects on laboratory strain of Ae. aegypti larvae were correlated with infusions and extract concentrations. Chopped plant infusions in hot water indicated mortality up to 77.3% of larvae. Ground plant infusions killed all of exposed larvae at day 3 postexposure. Median lethal concentrations (LC50,s) of chopped and ground plant infusions were 10.25 and 7.54%, respectively. Ethanolic extract had very strong effect on experimental subjects. Within 72 hours, 100% of laboratory strain of Ae. aegypti larvae died after exposure to extract at 100 parts per million (ppm) or higher concentrations. Ethanolic plant extract showed similar larvicidal effect on field strains of Ae. aegypti and Ae. albopictus. The percentage mortality of field strains larvae reached 100% after exposure to 100 ppm of plant extract. At concentrations of 1000 ppm, 100% of exposed larvae died with 8 hours. LC50 on tested larvae was 25.07-33.60 ppm. Strong larvicidal activity of S. nigrum suggests the possible application in DHF vector control effort.

scholarly journals Antifungal Activity of Beluntas “Indian Camphorweed” (Pluchea indica) Ethanol Extract on Candida albicans In Vitro Using Different Solvent Concentrations

2021 ◽  
Author(s):  
Wayan Larissa Demolsky ◽  
Vinna Kurniawati Sugiaman ◽  
Natallia Pranata
Keyword(s):  
Candida Albicans ◽  
Oral Candidiasis ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Pathogenic Fungi ◽  
Inhibitory Effect ◽  
Plate Count ◽  
Minimum Fungicidal Concentration ◽  
Significant Difference

Abstract Objective Oral candidiasis is an infection caused by pathogenic fungi Candida albicans, with a considerably high prevalence of 20 to 72%. Indian camphorweed (Pluchea indica) also known as “beluntas” as the local name has been known as a traditional medicine in Indonesia. The objective of this study is to research the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of beluntas ethanolic extract against the growth of C. albicans. Materials and Methods The MIC and MFC were measured by microdilution assay and total plate count respectively with a variation of solvents (DMSO 1%, 10%, and 4%) and beluntas extract with concentrations between 0.3125 and 200 mg/mL. Amphotericin and nystatin were used as a comparison. Statistical Analysis One-way analysis of variance and posthoc Tukey test were used to determine the significant difference between treatments. Results It was found that the MIC ranged from 50 to 200 mg/mL in the test with DMSO 10% solvent and MFC was found to be at a concentration of 200 mg/mL. However, there is a significant inhibitory effect and killing effect from DMSO 10% against C. albicans (p = 0.000). MIC was also found within concentrations of 100 mg/mL of beluntas extract in DMSO 4%. In this study, the DMSO 4% concentration neither showed significant inhibitory effects nor killing effects; therefore, the result was acceptable (p = 0.357). Conclusion Ethanol extract of beluntas (P. Indica) has the potential of being an antifungal agent with inhibitory activity in concentrations ≥100 mg/mL, which is similar to nystatin (p = 0.278). The MFC for the extract was above 100 mg/mL, which cannot be measured with this method as a higher concentration of DMSO is needed, which had a toxic effect on the tested fungi.

scholarly journals Ovicidal and Larvicidal Activities of Ethanolic Leaf Extracts of Three Botanicals Against the Malaria Vector - Anopheles Gambiae

2020 ◽  
Vol 9 (1) ◽  
pp. 111-121
Author(s):  
Funmilayo Bukola Egunjobi ◽  
Ikem Chris Okoye
Keyword(s):  
Anopheles Gambiae ◽  
Larval Mortality ◽  
High Rate ◽  
Pennisetum Purpureum ◽  
Health Concern ◽  
Phytochemical Analysis ◽  
Hatching Rate ◽  
Leaf Extracts ◽  
Tridax Procumbens ◽  
Larvicidal Activities

Malaria, transmitted by Anopheles gambiae, has been a major public health concern in Africa. Chemicals used in the control of A. gambiae have caused a lot of havoc in the environment and to non-target organisms. More so, a high rate of resistance by these mosquitoes has been recorded. This study evaluated the ovicidal and larvicidal activities of ethanolic leaf extracts of Duranta erecta, Tridax procumbens and Pennisetum purpureum against A. gambiae. Phytochemical analysis of these plants revealed the presence of tannins, saponins, alkanoids, flavonoids, glycosides and anthroquinone. Ground dry leaves of each plant material were concentrated in 7 litres of 95% ethanol for 72 hours followed by filtration and evaporation. D. erecta, T. procumbens and P. purpureum yielded 617.2g, 598.3g and 552g of extracts respectively. The WHO standard for mosquito bioassay was adopted and concentrations 40, 100, 140 and 200 parts per million (PPM) were tested against 20 eggs and 25 larvae using emersion method. The hatching rate and % larval mortality of the extracts were recorded in which a concentration dependent increase was observed. High ovicidal activity (low egg hatchability) was recorded in D. erecta (LC50 -10.037 PPM) followed by P. purpureum and T. procumbens with LC50 values of 17.380 and 39.198 respectively. The highest larvicidal activity was observed in D. erecta (LC50 -76.943 PPM) compared to P. purpureum and T. procumbens (LC50 - 213.410 PPM and 214.217 PPM). Evidently, D. erecta ethanolic leaf extracts showed the best efficacy in the control of A. gambiae in this study. D. erecta is an environmentally friendly alternative in reducing the use of chemicals for mosquito control.

scholarly journals Screening of Different Extracts of Marine Macro Green Algae for Larvicidal Activity against Dengue Fever Mosquito, Aedes aegypti (Diptera: Culiadae)

2017 ◽  
Vol 62 ◽  
pp. 44-51
Author(s):  
G. Adaikala Raj ◽  
M. Jayaraman ◽  
S. Krishnamoorthy ◽  
M. Chandrasekaran ◽  
V. Venkatesalu
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Green Algae ◽  
Larvicidal Activity ◽  
Ethyl Acetate Extract ◽  
Larval Mortality ◽  
Control Agent ◽  
Probit Analysis ◽  
Mosquito Vector ◽  
Ethyl Acetate Extracts

The present study larvicidal activities of hexane, chloroform, ethyl acetate, acetone and methanol extracts of Halimeda macroloba, Decsne, Caulerpa racemosa (Frosk) Weber-Van-Bosses and Ulva lactuca Lin, (Chlorophyceae) against Aedes aegypti. The marine macro green algae extracts were tested against early 4th instar larvae of A. aegypti using WHO protocol and concentrations of 200, 400, 600, 800 and 1000 ppm. The observed mortality was made 24 and 48 h after treatment, data was subjected to probit analysis to determine the lethal concentration (LC50 and LC90) of the treated larvae of the tested species.Among the tested extracts the maximum efficicacy was observed in the ethyl acetate extracts. The ethyl acetate extracts of the seaweeds showed the presence of terpenoids, tannins and phenolic compounds stronger than the other extracts. The results revealed that all the extracts showed varied levels of larvicidal activity against A. aegypti tested. However, the ethyl acetate extract of C. racemosa showed remarkable larvicidal activity against A. aegypti (LC50=579.9 and LC90=1255.4 ppm values at 24 h and LC50 =495.4 and LC90 =1073.9 ppm at 48 h) followed by U. lactuca (LC50=588.1 and LC90=1290.7 ppm values at 24 h, and LC50= 530.8 and LC90= 1160.0 ppm at 48 h), respectively. The lowest larval mortality was observed with hexane extract of H. macroloba against A. aegypti with values of LC50=1116.8 and LC90= 1824.5 ppm (after 24 h) and LC50=1059.9 and LC90=1768.3 ppm (after 24 h). The present studies indicate that the larvicidal activity and phytochemicals derived from the ethyl acetate extract of C. racemosa have the potential to be used as an ideal eco-friendly approach and effective mosquito vector control agent.

scholarly journals Antioedematous and Analgesic Properties of Fertile Fronds of Drynaria quercifolia

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
G. I. Anuja ◽  
P. G. Latha ◽  
V. J. Shine ◽  
S. R. Suja ◽  
P. Shikha ◽  
...  
Keyword(s):  
Biological Response ◽  
Ethanolic Extract ◽  
Dry Weight ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Therapeutic Effects ◽  
Hot Plate Test ◽  
Standard Procedures

Inflammation is a complex biological response of tissue cells to harmful stimuli including trauma, tissue necrosis, and infections which plays a key role in the pathophysiology of many deadly diseases. In ethnomedicine Drynaria quercifolia fronds are used to treat inflammation as poultice on swellings and as antibacterial, hepatoprotective, and antipyretic agent. Herein, we have evaluated the antioedematous, antiproliferative, and analgesic properties of the ethanolic extract of fertile fronds of D. quercifolia (FF) by standard procedures. Oral administration of FF produced significant inhibition of carrageenan and histamine induced paw oedema in Wistar rats. FF significantly reduced both wet weight and dry weight of granuloma tissue which shows the inhibitory effect on exudative and proliferative phases of inflammation. FF significantly attenuated acute and delayed phases of formalin induced pain, acetic acid-induced writhing, capsaicin-induced nociception, and hot plate test in mice. Phytochemical analysis revealed the presence of coumarins, flavonoids, glycosides, phenolics, saponins, steroids, tannins, and terpenoids. Total phenolic content was 186 mg/g equivalent of gallic acid. The HPLC estimation showed flavanone glycoside naringin (1.2%) and its aglycone naringenin (0.02%). The presence of potent anti-inflammatory and analgesic principles in FF and their synergistic action may be the reason for the proposed therapeutic effects.

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