THE EFFECT OF SOME OF COCONUT WATER CONCENTRATION IN ARTIFICIAL MEDIA TO CHRYSANTHEMUM GROWTH (Dendranthema grandiflora) BY IN VITRO

Artificial medium for in vitro culture is an alternative media to subtitute Murashige and Skoog (MS). Growmore added with organic substances as coconut water can be used as artificial medium. Coconut water can affect plant growth, so the research is to investigate the influence of coconut water in different concentration to chrysanth’s (Dendranthema grandiflora) growth should be done. The research was conducted in Laboratory of Molecular and Cell Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Syiah Kuala University Darussalam, Banda Aceh, from April until July 2013. The research was designed by using Randomized Block Design with sub sampling. Media act as group, while coconut water concentration as treatment. Observation was started from growing time up to a month after planting. The result showed that there was no significant different between using MS and artificial medium with different coconut water concentration to initiate shootlet.

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RESPONS PERTUMBUHAN TUNAS MIKRO STEVIA (Stevia rebaudiana Bertoni) SECARA IN VITRO PADA BEBERAPA JENIS SITOKININ DAN KONSENTRASI AIR KELAPA

Agrin ◽

10.20884/1.agrin.2017.21.2.395 ◽

2018 ◽

Vol 21 (2) ◽

Author(s):

Sepdian Luri Asmono ◽

Vega Kartika Sari ◽

Rudi Wardana

Keyword(s):

Shoot Growth ◽

Block Design ◽

Water Concentration ◽

Stevia Rebaudiana ◽

Coconut Water ◽

Node Number ◽

Stevia Rebaudiana Bertoni ◽

Shoot Number ◽

Number Of Shoots

Penelitian ini bertujuan mengetahui pengaruh penggunaan beberapa jenis sitokinin dan beberapa levelkonsentrasi air kelapa terhadap respon pertumbuhan tunas mikro stevia. Rancangan Penelitian ini menggunakanRancangan Acak Lengkap (RAL), meliputi 3 jenis sitokinin (2 ppm Kinetin, 2ppm BAP, 2ppm TDZ) dan 4 levelkonsentrasi air kelapa (0%; 5%; 10%; 15%) dengan 5 ulangan. Parameter pengamatan meliputi persentasekontaminasi, persentase browning, saat muncul tunas, jumlah tunas, panjang tunas dan jumlah ruas. Data dianalisisdengan analisis ragam (ANOVA) dan uji DMRT pada taraf 5%. Hasil penelitian pada 30 HST untuk parametersaat muncul tunas, jumlah tunas, panjang tunas dan jumlah ruas, interaksi antara BAP dan air kelapa menunjukkanperbedaan yang tidak nyata. Namun hasil analisis menunjukkan pengaruh tunggal sitokinin dan air kelapa dalampembentukan dan pertumbuhan tunas. Media perlakuan dengan menggunakan MS+2ppm BAP tanpa air kelapaterbukti memacu kemunculan tunas lebih cepat, yaitu rata-rata 3,05 Hari Setelah Kultur (HSK) serta memacupertambahan jumlah ruas (2,6 ruas per tunas) dan tunas (3,7 tunas per eksplan). Penambahan air kelapamenghambat kecepatan pertumbuhan tunas. Parameter pertumbuhan tunas menunjukkan semakin besarkonsentrasi air kelapa yang ditambahkan semakin menurunkan pertumbuhan tunas baik dari jumlah, panjang atauruas.Kata kunci: Stevia, in vitro, air kelapa, sitokininABSTRACTThis study aimed to determine effect of use of several types of cytokinin and levels of coconut waterconcentration on stevia micro shoot growth response. This study was arranged in a Randomized Block Design,including 3 types of cytokines (2 ppm Kinetin, 2ppm BAP, 2ppm TDZ) and 4 levels of coconut water concentration(0%, 5%, 10%, 15%) with 5 replications. The parameters included percentage of contamination, percentage ofbrowning, time to form shoots, number of shoots, shoot length and number of nodes. Analyzed by variance analysis(ANOVA) and DMRT test at 5% level. For analysis results of time to form shoots, number of shoots, shoot lengthand number of segments showed that the interaction between BAP and coconut water gave no significantdifference. However, the results of the analysis showed a single effect of cytokinin and coconut water in shootformation and growth. Treatment media using MS + 2ppm BAP without coconut water proved to accelerate theappearance of shoot more rapidly, that is average 3.05 days after culture and induced the increase of the numberof node (2.6 node per shoot) and shoot number (3.7 shoots per explant). The addition of coconut water inhibitedthe growth rate of buds. Shoot growth of the number, length, and node number of shoots decline due to highconcentration of coconut water added further decrease the growth of shoot either from the number, length, andnode number of shoots.Key words: Stevia, in vitro, coconut water, cytokinins

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Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v6i1.5449 ◽

2019 ◽

Vol 6 (1) ◽

pp. 78

Author(s):

Muhammad Ade Salim ◽

Muhammad Nur Ihsan ◽

Nur Isnaini ◽

Trinil Susilawati

Keyword(s):

Semen Quality ◽

Block Design ◽

Animal Husbandry ◽

Egg Yolk ◽

Coconut Water ◽

Boer Goat ◽

Randomized Block Design ◽

Frozen Semen ◽

Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari 3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1: young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%), while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

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Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽

10.30598/a.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

S. Tuhuteru ◽

Meity L Hehanussa ◽

Simon H.T Raharjo

Keyword(s):

Culture Medium ◽

Culture Media ◽

Water Concentration ◽

Medium Composition ◽

Coconut Water ◽

Orchid Species ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

Jurnal SAINS ALAMI (Known Nature) ◽

10.33474/j.sa.v3i2.7222 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Wulan Dari Neng Gumiwang ◽

Tintrim Rahayu ◽

Ari Hayati

Keyword(s):

Root Length ◽

Cocos Nucifera ◽

Water Concentration ◽

Coconut Water ◽

Culture Bottle ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%) dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan

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Nutritional value of sorghum silages

Acta Scientiarum Animal Sciences ◽

10.4025/actascianimsci.v39i2.32677 ◽

2017 ◽

Vol 39 (2) ◽

pp. 137

Author(s):

Marielly Maria Almeida Moura ◽

Daniel Ananias de Assis Pires ◽

Renê Ferreira Costa ◽

Daniella Cangussú Tolentino ◽

João Paulo Sampaio Rigueira ◽

...

Keyword(s):

Crude Protein ◽

Block Design ◽

Significance Level ◽

Dry Matter Digestibility ◽

Randomized Block Design ◽

Nutritional Characteristics ◽

High Participation ◽

Sorghum Genotypes

This study aimed to evaluate the nutritional characteristics and quality of silages of five sorghum genotypes, namely: Volumax, AG2005E, Qualimax, BRS610 and AG2501. The study was conducted at the Experimental Farm Unimontes, in the municipality of Janaúba, state of Minas Gerais. The treatments were distributed in a randomized block design with four replicates. Data were tested by analysis of variance andmeans were compared by Scott-Knott test at a significance level of 5%. The pH values ranged from 3.93 and 4.10. Genotypes differed in all studied nutritional characteristics (p < 0.05). For the acid detergent fiber, differences were detected between the genotypes, AG2501 (35.66%), Volumax (34.89%), AG2005E (34.53%), Qualimax (32.39%) and BRS610 (33.60%). The high participation of lignin was verifiedin all genotypes except for Volumax (4.14%). All silages were within the recommended range 50-65% for in vitro dry matter digestibility and classified as having good quality, however only the AG2005E genotype met nitrogen requirements for microbial fermentation, with 7.06% crude protein.

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Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA

Journal of Tropical Crop Science ◽

10.29244/jtcs.4.3.102-107 ◽

2017 ◽

Vol 4 (3) ◽

pp. 102-107

Author(s):

Darda Efendi ◽

Mirza R Putra

Keyword(s):

Carica Papaya ◽

Clonal Propagation ◽

Block Design ◽

Shoot Multiplication ◽

Plant Materials ◽

Randomized Block Design ◽

Lateral Shoots ◽

Shoot Production ◽

Set Up

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

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Uji Efektivitas Rizobakteria Dalam Menghambat Perkembangan Penyakit Hawar Pelepah Daun (Rhizoctonia solani Kuhn.) Pada Padi Secara In Vitro

EnviroScienteae ◽

10.20527/es.v16i1.8999 ◽

2020 ◽

Vol 16 (1) ◽

pp. 44

Author(s):

Hanisa Desy Ariani ◽

Noor Aidawati ◽

Dewi Arika Adriani

Keyword(s):

Pseudomonas Aeruginosa ◽

Rhizoctonia Solani ◽

Plant Protection ◽

Block Design ◽

Sheath Blight ◽

In Vitro Test ◽

Sheath Blight Disease ◽

Randomized Block Design ◽

Blight Disease

One of the causes of the declining productivity of rice is sheath blight disease caused by the mold Rhizoctonia solani Kuhn. Control of sheath blight disease that is often done by the farmers is by using chemical pesticides (fungicides), which caused environmental problems. One way to reduce the use of pesticides is to biological control by using antagonist bacteria. This study aimed at in vitro test of rhizobacteria in preventing the development of sheath blight disease in rice plants. This research was conducted in the Phytopathology laboratory of Plant Protection Department of Faculty Agriculture, University of Lambung Mangkurat Banjarbaru from March to May 2018. The experiment used a randomized block design with three groups consisting of eight types of rhizobacteria isolates: (r1) Pseudomonas aeruginosa (Barito Kuala), (r2) Bacillus megaterium (Hulu Sungai Tengah), (r3) Azotobacter sp. (Barito Kuala), (r4) Pseudomonas sp. (Hulu Sungai Selatan), (r5) Flavobacterium sp. (Tanah Laut), (r6) Bacillus bodius (Barito Kuala), (r7) Pseudomonas aeruginosa (Hulu Sungai Selatan), (r8) Necercia sp. (Tanah Laut). The results showed that all rhizobacteria have the ability to inhibit the development of R. solani with different percentages of inhibitions. Pseudomonas aeruginosa (Barito Kuala) was the most effective rhizobacteria in inhibiting the development of R. solani.

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Variabilitas Genotipe-Genotipe Mutan Krisan (Dendranthema grandiflora Tzvelv.) Generasi MV5 Hasil Irradiasi Sinar Gamma

Agrikultura ◽

10.24198/agrikultura.v27i3.10881 ◽

2016 ◽

Vol 27 (3) ◽

Author(s):

Dedeh Kurniasih ◽

Dedi Ruswandi ◽

Murdaningsih Haeruman Karmana ◽

Warid Ali Qosim

Keyword(s):

Experimental Method ◽

Plant Height ◽

Gamma Ray ◽

Phenotypic Variability ◽

Block Design ◽

Mutation Breeding ◽

Dendranthema Grandiflora ◽

Randomized Block Design ◽

Flower Diameter ◽

Flower Type

ABSTRACTVariability of mutant genotypes chrysanthemum (Dendranthema grandiflora Tzvelv.) fifth generationsthrough gamma iIrradiationDendranthema grandiflorum Tzvelv. is a major floriculture in Indonesia, and it is one of the five most popular flowers in Indonesia. Chrysanthemum varieties in Indonesia is largely the introduced varieties. Chrysanthemum hybridization especially for decorative flower type in order to obtain superior varieties is relatively difficult, so the mutation breeding is one approach that can be taken to get the chrysanthemum varieties with different phenotypic performances with the that parent.The purpose of this study was to obtain information genetic and phenotypic variability characters observed on chrysanthemum irradiated with gamma ray. The experiment was conducted by an experimental method using a randomized block design (RBD). The treatments consisted of 37 mutants genotypes and 11 genotypes chrysanthemums parent as controls with two replications. The results of this study indicated that the genotypes tested had broad genetic and phenotipic variation for the plant height, flower diameter, number of flower and neck lengths.Key words: Chrysanthemum mutants, Variability, Gamma ray irradiation.ABSTRAKKrisan merupakan komoditas tanaman hias utama di Indonesia dan paling banyak diminati masyarakat. Varietas-varietas krisan yang beredar di Indonesia sebagian besar merupakan varietas introduksi. Persilangan krisan khususnya untuk tipe bunga dekoratif dalam rangka memperoleh varietas unggul relatif sulit dilakukan, sehingga pemuliaan mutasi merupakan salah satu pendekatan yang dapat ditempuh untuk mendapatkan varietas krisan dengan penampilan fenotipik yang berbeda dengan induknya. Tujuan penelitian ini adalah untuk mendapatkan informasi variabilitas genetik dan fenotipik karakter-karakter yang diamati pada tanaman krisan yangd iradiasi dengan sinar gamma. Percobaan dilakukan dengan metode eksperimen menggunakan Rancangan Acak Kelompok (RAK). Perlakuan terdiri atas 37 genotipe mutan krisan dan 11 genotipe tetua krisan sebagai kontrol dengan dua ulangan. Hasil penelitian menunjukkan bahwa genotipe-genotipe yang diuji memiliki variabilitas yang luas untuk karakter tinggi tanaman, diameter bunga, jumlah kuntum dan panjang tangkai bunga.Kata kunci: Mutan krisan, Variabilitas, Sinar gamma

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THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽

10.31315/agrivet.v27i1.4694 ◽

2021 ◽

Vol 27 (1) ◽

pp. 39

Author(s):

Salma Nabila ◽

Endah Budi Irawati ◽

Rina Srilestari

Keyword(s):

Water Concentration ◽

Coconut Water ◽

Fresh Weight ◽

Randomized Design ◽

Two Factors ◽

Completely Randomized Design ◽

Range Test ◽

Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

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The Effect of Processing and Cooling Methods on Coleus tuberosus in vitro Starch Digestibility

Jurnal Agritech ◽

10.22146/agritech.44596 ◽

2021 ◽

Vol 41 (3) ◽

pp. 238

Author(s):

Jhauharotul Muchlisyiyah ◽

Tri Dewanti Widyaningsih ◽

Retno Wulansari ◽

Hera Sisca Prasmita

Keyword(s):

Resistant Starch ◽

Room Temperature ◽

Potato Starch ◽

Block Design ◽

In Vitro Digestibility ◽

Randomized Block Design ◽

Two Factors ◽

Cooling Methods ◽

Total Starch

Coleus tuberosus, also known as black potato, is one of the Indonesian local tubers consumed as a carbohydrate substituent. Therefore, this study aimed to examine the effect of processing and cooling methods on the in vitro digestibility of black potato starch. Furthermore, two factors Randomized Block Design with a 2x3 experimental design was used, which consisted of processing methods (boiling, roasting, and microwave) and cooling at room temperature and 4 °C for 24 hours with 3 repetitions. Black potato flour was compared with the raw form, by assessing some parameters, namely Resistant Starch (RS), Slowly Digestible Starch (SDS), Rapidly Digestible Starch (RDS), and Glycemic Index (GI). Also, the analysis of total starch, moisture, and color was performed, hence raw black potatoes generally have 10% resistant starch (%wb). Different treatments of cooking and cooling had a significant effect (α = 0.05) on moisture content, total starch, RS, RDS, SDS, GI, brightness (L), and yellowness (b). Black potatoes subjected to the processing method followed by cooling had lower RDS and increased RS content. Furthermore, refrigeration at 4°C for 24 hours reduced the digestibility of black potato starch more than cooling at room temperature. Contrarily, microwaved black potato cooled at room temperature showed a higher digestion rate compared to the raw counterpart. Conclusively, processing followed by cooling reduces the GI and increases the RS content of Coleus tuberosus.

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