scholarly journals Radiosensitivitas dan Heritabilitas Ketahanan terhadap Penyakit Antraknosa pada Tiga Populasi Cabai yang Diinduksi Iradiasi Sinar Gamma

2016 ◽  
Vol 43 (3) ◽  
pp. 201
Author(s):  
Nura , ◽  
Muhamad Syukur ◽  
Nurul Khumaida ◽  
Widodo ,

<p>ABSTRACT</p><p>Commercial varieties of chili is still susceptible to anthracnose disease. Anthracnose is one of diseases which caused yield loss about 10-80% in rainy season and 2-35% in dry season. One of the disease control method is to develop new varieties resistant to anthracnose through gamma-ray irradiation. This research was aimed to determine radiosensitivity level and heritability of chili which generated by mutation inductions of three genotypes: IPB C2, IPB C10, and IPB C15. Seed of the three genotypes were irradiated by gamma rays at dosages of 100, 200, 300, 400, 500, 600, 700, 800, 900 and 1,000 Gy. The LD50 in IPB C2, IPB C10, and IPB C15 were 317.9, 591.4, and 538.8 Gy, respectively. The character of IPB C2 mutants ranged between very susceptible to resistant against anthracnose disease, IPB C10 tended to very susceptible to highly resistant, and genotype IPB C15 tended to susceptible to highly resistant. Genotype IPB C2 had high level in heritability for disease resistance character, while medium level in heritability value was obtained on genotype IPB C10 and IPB C15.</p><p>Keywords: genotype, LD50, mutant, mutation, seed</p>

2016 ◽  
Vol 35 (10) ◽  
pp. 1133-1144
Author(s):  
T Chen ◽  
L Wang ◽  
K Chen ◽  
S Qiu ◽  
X Cen ◽  
...  

To provide support for future pharmacology and preclinical studies, we have established a stable nonhuman primate animal model to demonstrate the histopathological changes in the gastrointestinal tract following gamma ray irradiation. In this study, 12 healthy rhesus monkeys were divided into 2 groups (control and radiation groups). Animals in the radiation group were exposed to gamma rays (cobalt 60 source) at a dose level of 6.5 Gy total body irradiation bilaterally (i.e. 3.25 Gy on each side). Control animals were sham exposed using identical procedures. After a 5-day in-life observation period, gastrointestinal tract tissues (esophagus, stomach, duodenum, jejunum, ileum, colon, and rectum) were collected and fixed in 10% neutral-buffered formalin for subsequent hematoxylin and eosin and 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry processing. The results showed that the esophagus was undergoing degeneration without obvious inflammatory changes, while the stomach and duodenum exhibited both degeneration and inflammation. From the jejunum to the rectum, late-stage inflammation with glandular regeneration, as well as a high-level BrdU labeling index, was present.


Weed Science ◽  
1994 ◽  
Vol 42 (4) ◽  
pp. 528-533 ◽  
Author(s):  
R. Baziramakenga ◽  
Gilles D. Leroux

Field trials were carried out in 1989 and 1990 at St-Augustin, near Québec City, Canada, to determine the critical periods of quackgrass control in potato submitted to three levels of infestation. Potato yield losses due to quackgrass interference increased with quackgrass infestation and length of duration of interference. Quackgrass interference influenced marketable tuber yields more than total tuber yields. Duration of the critical period varied depending on the level of quackgrass infestation and year. Based on an arbitrary 5% level of marketable yield loss, the critical period started at ca. 15 days after emergence (DAE) of potato at low level of infestation, and at ca. 3 DAE at medium level of infestation. At high level of infestation, the critical period began prior to the emergence of potato. The end of the critical period of quackgrass removal was extremely variable across quackgrass infestation level and year and ranged from 23 to 68 DAE of potato at a 5 % yield loss level. It appears that onset of interference varied less than the end of it, indicating that early quackgrass control is necessary to prevent yield loss.


Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 111 ◽  
Author(s):  
Jin-Oh Jeong ◽  
Jong-Seok Park ◽  
Young-Ah Kim ◽  
Su-Jin Yang ◽  
Sung-In Jeong ◽  
...  

Conducting polymer (CP)-based hydrogels exhibit the behaviors of bending or contraction/relaxation due to electrical stimulation. They are similar in some ways to biological organs and have advantages regarding manipulation and miniaturization. Thus, these hydrogels have attracted considerable interest for biomedical applications. In this study, we prepared PPy/PVP hydrogel with different concentrations and content through polymerization and cross-linking induced by gamma-ray irradiation at 25 kGy to optimize the mechanical properties of the resulting PPy/PVP hydrogel. Optimization of the PPy/PVP hydrogel was confirmed by characterization using scanning electron microscopy, gel fraction, swelling ratio, and Fourier transform infrared spectroscopy. In addition, we assessed live-cell viability using live/dead assay and CCK-8 assay, and found good cell viability regardless of the concentration and content of Py/pTS. The conductivity of PPy/PVP hydrogel was at least 13 mS/cm. The mechanical properties of PPy/PVP hydrogel are important factors in their application for biomaterials. It was found that 0.15PPy/PVP20 (51.96 ± 6.12 kPa) exhibited better compressive strength than the other samples for use in CP-based hydrogels. Therefore, it was concluded that gamma rays can be used to optimize PPy/PVP hydrogel and that biomedical applications of CP-based hydrogels will be possible.


2018 ◽  
Vol 53 ◽  
pp. 01007
Author(s):  
Yinghong Zuo ◽  
Maoyu Zhang ◽  
Guoxin Cheng ◽  
Shengli Niu

To obtain the transient current response law of the metal component irradiated by pulsed gamma rays, the pulsed gamma ray irradiation experiment of the iron plate was carried out on “Qiangguang-I” accelerator. The transient current of iron plate generated by pulsed gamma rays was measured and analysed, and the relationship between the amplitude of pulse current and the dose rate of gamma rays was obtained. The results show that the current response sensitivity of the iron plate is about 5.7×10-7(A/m2)/(Gy/s) when the gamma rays with the energy of 0.8 MeV irradiate the iron plate. The charge deposition rate in the iron plate can be obtained by Monte Carlo simulation, and then it can be converted to gamma ray induced current of the metal component irradiated by gamma rays.


2008 ◽  
Vol 600-603 ◽  
pp. 707-710
Author(s):  
Shigeomi Hishiki ◽  
Naoya Iwamoto ◽  
Takeshi Ohshima ◽  
Hisayoshi Itoh ◽  
Kazu Kojima ◽  
...  

The effect of the fabrication process of n-channel 6H-SiC MOSFETs on their radiation resistance is investigated. MOSFETs that post implantation annealing for source and drain was carried out with carbon coating on the sample surface are compared to MOSFETs that post implantation annealing was carried out without carbon coating. The radiation resistance (gamma-rays) of the carbon-coated MOSFETs is higher than that of non-coated ones. The channel mobility for MOSFETs whose gate oxide was formed using pyrogenic or dry oxidation process dose not change by gamma-ray irradiation below 1x105 Gy. The value of channel mobility slightly increases with increasing dose above 1x105 Gy. No significant increase in irradiation induced interface traps is observed.


2011 ◽  
Vol 679-680 ◽  
pp. 362-365 ◽  
Author(s):  
Takeshi Ohshima ◽  
Naoya Iwamoto ◽  
Shinobu Onoda ◽  
Takahiro Makino ◽  
Shinji Nozaki ◽  
...  

Charge induced in 6H-SiC nMOS capacitors by 15 MeV oxygen ion microbeams was measured using Transient Ion Beam Induced Current (TIBIC) before and after gamma-ray irradiations. The peak amplitude of TIBIC signals decreases and the fall time increases with increasing number of incident ions. The decrease in the TIBIC peak eventually saturated. The TIBIC signal can be refreshed to its original shape by applying a positive bias of + 1V to gate oxide. Small decrease in both the peak amplitude of TIBIC signal and collected charge was observed due to gamma-ray irradiation.


Pastura ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Prihantoro, I. ◽  
Anandia, A. ◽  
Aryanto, A. T. ◽  
Setiana, M. A. ◽  
Karti, P. D. M. H.

Alfalfa (Medicago sativa L.) a high nutritious and palatability legume for ruminant. Constrain of alfalfa availability in Indonesia are due to the plant adaptability in tropical environment. Aim of the study was to measure the adaptation level of alfalfa that irradiated with gamma rays on a field scale. The study consisted of four types of plant sources, mutation plants with different gamma ray levels (0Gy, 200Gy, 300Gy and 400Gy). Plant tillers was analyzed with a complete randomized design with 3 replications, 20 plants per replication. Growth capability, leaf color, flowering time and pest attack level were analyzed descriptively. The results showed that alfalfa irradiated with 300 Gy significantly (p<0.05) produced highest tillers. Irradiated 300 Gy plant gave better result on growth capability, flowering capability, and number of not attack plant from pests. The higher level of gamma ray irradiation showed the changed in leaf color levels from dark green to light green. Keywords: alfalfa (Medicago sativa L.), mutation, gamma ray irradiation, field scale


2016 ◽  
Vol 43 (3) ◽  
pp. 235
Author(s):  
Arrin Rosmala ◽  
Nurul Khumaida ◽  
Dewi Sukma

<p>ABSTRACT</p><p>Handeuleum (Graptophyllum pictum L.Griff) is a medicinal plant widely used as a traditional medicine due to its benefecial content. Therefore, it should be developed as one of the leading Indonesian medicinal plants. The purpose of this research was to study the effect of gamma ray irradiation on morphological alteration and growth of Handeuleum accession from Bogor. The doses of gamma rays were 0, 15, 30, 45, 60, 75, 90, and 105 Gy. The results of the research showed that gamma-ray irradiation induced morphological changes and influences the growth of Handeuleum. Irradiation dose at rate of 105 Gy produced new leaf morphology in Handeuleum, namely cordate. Doses of 60, 75, 90, and 105 Gy produce stunted plants with stiff and yellowish-green leaves. The dose of 45 Gy produced relative green color index which was higher than the control plants.</p><p>Keywords: handeuleum, gamma irradiations</p>


2021 ◽  
Vol 12 ◽  
Author(s):  
Sang Woo Lee ◽  
Yu-Jeong Kwon ◽  
Inwoo Baek ◽  
Hong-Il Choi ◽  
Joon-Woo Ahn ◽  
...  

Protons may have contributed to the evolution of plants as a major component of cosmic-rays and also have been used for mutagenesis in plants. Although the mutagenic effect of protons has been well-characterized in animals, no comprehensive phenotypic and genomic analyses has been reported in plants. Here, we investigated the phenotypes and whole genome sequences of Arabidopsis M2 lines derived by irradiation with proton beams and gamma-rays, to determine unique characteristics of proton beams in mutagenesis. We found that mutation frequency was dependent on the irradiation doses of both proton beams and gamma-rays. On the basis of the relationship between survival and mutation rates, we hypothesized that there may be a mutation rate threshold for survived individuals after irradiation. There were no significant differences between the total mutation rates in groups derived using proton beam or gamma-ray irradiation at doses that had similar impacts on survival rate. However, proton beam irradiation resulted in a broader mutant phenotype spectrum than gamma-ray irradiation, and proton beams generated more DNA structural variations (SVs) than gamma-rays. The most frequent SV was inversion. Most of the inversion junctions contained sequences with microhomology and were associated with the deletion of only a few nucleotides, which implies that preferential use of microhomology in non-homologous end joining was likely to be responsible for the SVs. These results show that protons, as particles with low linear energy transfer (LET), have unique characteristics in mutagenesis that partially overlap with those of low-LET gamma-rays and high-LET heavy ions in different respects.


2020 ◽  
Vol 36 (1) ◽  
pp. 97
Author(s):  
Nandariyah Nandariyah ◽  
Endang Yuniastuti ◽  
Sukaya Sukaya ◽  
Sonia Ika Yudhita

<p><a name="_Hlk39513249"></a><span lang="EN-US">Raja Bulu is one of the banana varieties favored by the community because of its thick fruit flesh and sweet taste. However, its parthenocarpic characteristic and vegetative propagation make this banana variety has limited genetic variation. Attempt to improve the genetic variation was conducted through induced mutation breeding using gamma-ray mutagens. This research aimed to select M1V1 generation of Raja Bulu banana (<em>Musa paradisiaca</em> Linn.) obtained by gamma rays’ irradiation for their growth traits which are expected to produce banana varieties that have an early maturity and high yield. This study used a randomized complete block design without replication by observing the generative growth of each individual of Raja Bulu banana irradiated by gamma rays and without radiation as a control. The results showed that gamma-ray irradiation treatment caused Raja Bulu banana to be harvested earlier and produced higher fruit weight than controls. The gamma-ray irradiation had a random influence on Raja Bulu bananas. The 10 Gy gamma-ray irradiation dosage influenced the morphological diversity in the generative phase of Raja Bulu banana. The treatment of gamma irradiation resulted in 5 individual plants that flowered and matured earlier as compared to controls</span><span lang="IN">.</span></p>


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