scholarly journals Uji Aktivitas Ekstrak Daun Dan Akar Singawalang (Petiveria alliaceae) Terhadap Penghambatan Tirosinase

2021 ◽  
pp. 189
Author(s):  
Munawarohthus Sholikha ◽  
Nur M, R. ◽  
Nur M, R. ◽  
Mahfudza, A.R.
Keyword(s):  
Kojic Acid ◽  
Root Extract ◽  
Tyrosinase Inhibitor ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Root Extracts ◽  
Activity Test ◽  
Petiveria Alliacea ◽  
Elisa Technique ◽  
Microplate Reader

Tyrosinase is an enzyme that plays a role in the formation of skin pigments from a person because it is involved in the process of melanogenesis. Tyrosinase plays a very important role in the skin depigmentation process, there are several attempts to inhibit the skin depigmentation process, one of which is by inhibiting tyrosinase. Research on the leaves and roots of singawalang (Petiveria alliacea) was conducted to determine the potential as a tyrosinase inhibitor. Leaves and root extracts of singawalang were macerated with ethanol, then tested for identification of secondary metabolites. Singawalang leaves extract contains alkaloids, tannins and terpenoids while singawalang root extract contains alkaloids, flavonoids, tannins and terpenoids. Tyrosinase inhibitory activity used the microplate reader ELISA technique at a wavelength of 492 nm. Tests were carried out on kojic acid as a comparison and L-DOPA as a substrate. The results of the tyrosinase inhibition activity test for the extracts of singawalang leaves, singawalang roots and kojic acid, IC50 were 9.817 mg / mL, 4.987 mg / mL and 0.092 mg / mL, respectively.

scholarly journals In Vitro Enzyme Tyrosinase Inhibitory Activity Test on Liquorice Root Extract Cream (Glycyrrhiza glabra L.)

2018 ◽  
Vol 16 (2) ◽  
pp. 150
Author(s):  
Siti Umroh Noor ◽  
Faridah Faridah ◽  
Pamela Magdalena
Keyword(s):  
Kojic Acid ◽  
Ethanol Extract ◽  
Positive Control ◽  
Glycyrrhiza Glabra ◽  
Root Extract ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Skin Whitening ◽  
Glycyrrhiza Glabra L

Liquorice root extract (Glycyrrhiza glabra L.) contains glabridin, an isoflavone substance that has the ability to inhibit the oxidation of L-tyrosin and L-DOPA in the formation of melanin, therefore it can be used as skin whitening. The aim of the study was to determine the potential of the best skin whitening cream using positive control of kojic acid. Formulated 1 blank formula, 3 cream formulas using various concentrations of ethanol extract 70% liquorice roots of (0.01, 0.11, 1.01)% based on the value of tyrosinase inhibition activity in vitro from extracts of (50, 75, 100)%. Spectrophotometric method is used to measure the absorption of dopacrome by a microplate reader which was incubated at 37°C at λ 490 nm for 20 minutes. Oil in water cream was prepared by mixing extract with cream base at a temperature of 70-75°C at a speed of 400 rpm for 25 minutes. Stability test was carried out for 4 weeks at room temperature storage and a temperature of 40°C, evaluated for physical quality. The results of determination of IC50 of kojic acid was 20.88 µg / mL; IC50 of extract variation were (126.75; 1130.90; and 10092.41) µg / mL respectively. Cream has milky white-yellowish color; smell of flowers; soft texture; homogeneous; there is no separation; has type M/A; plastic thixotropic flow properties; has an increasing value including viscosity, spreadability, globule size, and pH, which are respectively (455000-620000)cPs, (2695.82-3545.83)mm2, (54.66-66.27)μm , and (4.44-5.04); tyrosinase inhibition activity in formula 1, 2 and 3 were stored at weeks 0, 2nd  and 4th  were respectively (1.78; -25.74; 22.04)%, (6.74; 6, 12; 4.49)%, and (-28.78; 53.06; 20.32)%. It can be concluded that the cream formula containing liquorice root extract with a concentration of 1.01% is the best formula so it can be used as skin whitening.

scholarly journals THE RECENT UPDATE OF DEOXYARBUTIN: A SKIN DEPIGMENTATION AGENT WITH TYROSINASE INHIBITION TARGETING

2020 ◽  
pp. 1-7
Author(s):  
MUCHTARIDI MUCHTARIDI ◽  
MENTARI LUTHFIKA DEWI
Keyword(s):  
Kojic Acid ◽  
Enzyme Inhibitor ◽  
Clinical Manifestations ◽  
Tyrosinase Inhibitor ◽  
Tyrosinase Inhibition ◽  
Considerable Potential ◽  
Abnormal Accumulation ◽  
Tyrosinase Enzyme ◽  
The Stability ◽  
Further Development

Melanin is produced through the process of melanogenesis, which serves to protect the skin from the damaging effects of UV radiation. Abnormal accumulation of melanin will aesthetically disturb even interfere with health. One of the clinical manifestations of abnormal accumulation of melanin is the incidence of melasma. Some of the tyrosinase enzyme inhibitor agents most widely used as Hydroquinone, Kojic acid and Arbutin do not give satisfactory results and cause serious side effects. Hydroquinone is known to cause ochronosis exogenous and cytotoxic. Kojic acid is known to cause allergies and mutagenic, while arbutinisis is known to have cytotoxic properties lower than hydroquinone, but less satisfactory depigmentation activity. There was a compound that has been synthesized by removing the hydroxy group of arbutin, known as deoxyarbutin (4-[Tetrahydro-2H-Pyrans-2-yl) oxy] phenol). Deoxyarbutin (dA) shows Ki 10-fold is lower than hydroquinone and 350-fold is lower than arbutin. IC50dA is 17.5+0.5 µmol/l, while the IC50 hydroquinone is 73.7+9.1 µmol/l. In terms of security, dA indicates that cell viability is 95% higher than hydroquinone. However, dA is thermolabile and photolabile. Several studies have shown satisfactory results to improve the stability of dA, that these compounds are considerable potential for further development as a depigmentation agent. The aim of this review is to describe how the potency of dA as a tyrosinase inhibitor interferes melanogenesis process through the latest depigmentation agent, its safety, efficacy and stability.

Petiveria alliacea root extract as Green Inhibitor for Acid Corrosion of Aluminium

2020 ◽  
Vol 45 (4) ◽  
Author(s):  
O.O. Adeyemi ◽  
A.O. Salisu
Keyword(s):  
Weight Loss ◽  
Acid Corrosion ◽  
Root Extract ◽  
Green Inhibitor ◽  
Isotherm Equation ◽  
Langmuir Adsorption ◽  
Root Extracts ◽  
Petiveria Alliacea ◽  
Polarization Studies ◽  
Mixed Type Inhibitor

Corrosion inhibition of aluminium in 0.5M 𝐻 􀀀􀀁􀀂 by methanol root extracts of Petiveria alliacea was investigated by weight loss and linear polarization techniques. The weight loss is concentration dependent and increases with increasing concentration of Petiveria alliacea root extracts (PARE). The inhibitor (PARE) exhibited highest inhibition efficiency of 75.28% at the highest inhibitor concentration of 8g investigated. Inhibition was found to increase with increasing PARE concentration. PARE showed molecular adsorption of phytochemicals present as active ingredients through the hetero atoms in their molecules. Polarization studies showed that the extracts act as a mixed type inhibitor. The inhibitor obeyed the Langmuir adsorption isotherm equation.

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

Synergistic Effects of Linderanolide B Combined with Arbutin, PTU or Kojic Acid on Tyrosinase Inhibition

2015 ◽  
Vol 16 (12) ◽  
pp. 1120-1126 ◽  
Author(s):  
You-Cheng Hseu ◽  
Kuo-Chen Cheng ◽  
Yi-Chieh Lin ◽  
Chung-Yi Chen ◽  
Hsin-Yu Chou ◽  
...  
Keyword(s):  
Kojic Acid ◽  
Synergistic Effects ◽  
Tyrosinase Inhibition

scholarly journals Cylindromicin from Arctic-Derived Fungus Tolypocladium sp. SCSIO 40433

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1080
Author(s):  
Imran Khan ◽  
Jing Peng ◽  
Zhuangjie Fang ◽  
Wei Liu ◽  
Wenjun Zhang ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Nuclear Overhauser Effect ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Bioactive Natural Products ◽  
Chemical Structures ◽  
Overhauser Effect ◽  
Spectroscopic Analyses ◽  
Potential Resource ◽  
Nuclear Overhauser

The fungus strain SCSIO 40433 was isolated from an Arctic-derived glacier sediment sample and characterized as Tolypocladium cylindrosporum. A new compound, cylindromicin (1), and seven known secondary metabolites (2–8) were isolated from this strain. The chemical structures of these compounds were elucidated by comprehensive spectroscopic analyses. Cylindromicin (1) featured a 3,4-dihydro-2H-pyran skeleton. The absolute configuration of compound 1 was assigned via interpretation of key Nuclear Overhauser Effect Spectroscopy (NOESY) correlations and Electronic Circular Dichroism (ECD) calculation. Cylindromicin (1) exhibited significant tyrosinase inhibition activity. This study highlights Polar fungi as a potential resource for new bioactive natural products.

scholarly journals Valonea Tannin: Tyrosinase Inhibition Activity, Structural Elucidation and Insights into the Inhibition Mechanism

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2747
Author(s):  
Jiaman Liu ◽  
Yuqing Liu ◽  
Xiaofeng He ◽  
Bo Teng ◽  
Jacqui M. McRae
Keyword(s):  
Molecular Weight ◽  
Structure Function ◽  
Hydrophobic Interactions ◽  
Average Molecular Weight ◽  
Commercial Application ◽  
Inhibition Mechanism ◽  
Resonance Spectroscopy ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Function Activity

Valonea tannin is a natural product readily extracted from acorn shells that has been suggested to have potential skin whitening properties. This study investigated the tyrosinase inhibition activity of extracted valonea tannin and the associated structure–function activity. Nuclear magnetic resonance spectroscopy and molecular weight analysis with gel permeation chromatography revealed that valonea tannin could be characterized as a hydrolysable tannin with galloyl, hexahydroxydiphenoyl and open formed-glucose moieties and an average molecular weight of 3042 ± 15 Da. Tyrosinase inhibition assays demonstrated that valonea tannin was 334 times more effective than gallic acid and 3.4 times more effective than tannic acid, which may relate to the larger molecular size. Kinetic studies of the inhibition reactions indicated that valonea tannin provided tyrosinase inhibition through mixed competitive–uncompetitive way. Stern–Volmer fitted fluorescence quenching analysis, isothermal titration calorimetry analysis and in silico molecule docking showed valonea tannin non-selectively bound to the surface of tyrosinase via hydrogen bonds and hydrophobic interactions. Inductively coupled plasma-optical emission spectroscopy and free radical scavenging assays indicated the valonea tannin had copper ion chelating and antioxidant ability, which may also contribute to inhibition activity. These results demonstrated the structure–function activity of valonea tannin as a highly effective natural tyrosinase inhibitor that may have commercial application in dermatological medicines or cosmetic products.

Tyrosinase Inhibitory Activity of Flavonoids from Artocarpus Lowii King

2014 ◽  
Vol 71 (1) ◽  
Author(s):  
Shajarahtunnur Jamil ◽  
Siti Awanis Abdullah ◽  
Siti Mariam Abdul Lathiff ◽  
Hasnah Mohd Sirat
Keyword(s):  
Inhibitory Activity ◽  
Kojic Acid ◽  
Mushroom Tyrosinase ◽  
Tyrosinase Inhibitory Activity ◽  
Crude Extracts ◽  
Ic50 Value ◽  
Microplate Reader

Tyrosinase inhibitory activity was studied on the crude extracts and flavonoids successfully isolated from the leaves and heartwoods of Artocarpus lowii King. The flavonoids were fully characterized spectroscopically as isobavachalcone (1), 4-hydroxyonchocarpin (2), 2',4'-dihydroxy-4-methoxy-3'-prenyldihydrochalcone (3), 2',4'-dihydroxy-3,4-(2",2"-dimethylchromeno)-3'-prenyldihydrochalcone (4), artocarpin (5), cycloheterophyllin (6) and 4',5-dihydroxy-6,7-(2,2-dimethylpyrano)-2'-methoxy-8-γ,γ-dimethyl allylflavone (7). Tyrosinase inhibitory activity of the samples was determined against mushroom tyrosinase using ELISA microplate reader. Cycloheterophyllin (6) exhibited an excellent inhibitory activity against mushroom tyrosinase comparable to the standard kojic acid with the IC50 value of 52.5 µg/mL (88.3%).

scholarly journals Enzyme-Site Blocking Combined with Optimization of Molecular Docking for Efficient Discovery of Potential Tyrosinase Specific Inhibitors from Puerariae lobatae Radix

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2612 ◽  
Author(s):  
Haichun Liu ◽  
Yitian Zhu ◽  
Ting Wang ◽  
Jin Qi ◽  
Xuming Liu
Keyword(s):  
Molecular Docking ◽  
Structural Modification ◽  
Enzyme Inhibitors ◽  
Kojic Acid ◽  
Systematic Evaluation ◽  
Tyrosinase Inhibition ◽  
Computational Docking ◽  
Site Blocking ◽  
Leading Compounds ◽  
Positive Results

Enzyme inhibitors from natural products are becoming an attractive target for drug discovery and development; however, separating enzyme inhibitors from natural-product extracts is highly complex. In this study, we developed a strategy based on tyrosinase-site blocking ultrafiltration integrated with HPLC-QTOF-MS/MS and optimized molecular docking to screen tyrosinase inhibitors from Puerariae lobatae Radix extract. Under optimized ultrafiltration parameters, we previously used kojic acid, a known tyrosinase inhibitor, to block the tyrosinase active site in order to eliminate false-positive results. Using this strategy, puerarin, mirificin, daidzin and genistinc were successfully identified as potential ligands, and after systematic evaluation by several docking programs, the rank of the identified compounds predicted by computational docking was puerarin > mirificin > kojic acid > daidzin ≈ genistin, which agreed with the results of tyrosinase-inhibition assays. Structure-activity relationships indicated that C-glycosides showed better tyrosinase inhibition as compared with O-glycosides, with reduced inhibition achieved through the addition of glycosyl, which provides ideas about the screen of leading compounds and structural modification.

Investigation of tyrosinase inhibition by some 1,2,4 triazole derivative compounds: in vitro and in silico mechanisms

2018 ◽  
Vol 44 (4) ◽  
pp. 473-481
Author(s):  
Elif Ayazoglu Demir ◽  
Ahmet Colak ◽  
Aylin Kalfa ◽  
Ahmet Yasar ◽  
Olcay Bekircan ◽  
...  
Keyword(s):  
Critical Role ◽  
Docking Studies ◽  
Biosynthesis Pathway ◽  
Mushroom Tyrosinase ◽  
Tyrosinase Inhibitor ◽  
Effective Inhibitor ◽  
Tyrosinase Inhibition ◽  
Triazole Derivative

Abstract Background Tyrosinase plays a central role in the biosynthesis pathway of melanin pigment. Melanin protects human skin against radiation and its unusual levels cause some skin disorders such as pregnancy scar, oldness spots and melanoma. Tyrosinase has also been linked to Parkinson’s and other neurodegenerative diseases. In addition, melanin plays a critical role as a defense molecule for insects during wound healing and is important for their life. Therefore, determination of inhibitor molecules for tyrosinase has a promising potential for therapies of some diseases and is an alternative method for keeping insects under control. Material and methods In this study, 1-hepthyl-3-(4-methoxybenzyl)-4H-1,2,4-triazole-5-one derivative (A6, A8, A15) and 3-(4-chlorophenyl)- 5-(4-methoxybenzyl)-4H-1,2,4-triazole (B5, B9, B13) derivative compounds were evaluated in terms of their potential for mushroom tyrosinase inhibition. IC50 values of these six molecules were determined. Results It was seen that B9 molecule was the most effective inhibitor. Docking studies also nearly supported this end result. Tyrosinase inhibition type and Ki value were found to be uncompetitive and 370.7±0.3 μM, respectively, in the presence of B9 compound. Conclusion These results suggest that B9 compound is a potential tyrosinase inhibitor.

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