THE CONTENT OF DDR1 COLLAGEN RECEPTORS IN THE BLOOD SERUM OF CHILDREN WITH CHRONIC PYELONEPHRITIS: CONNECTION WITH THE URODYNAMICS DISRUPTION AND METABOLIC DISORDERS

Over the past decades, there has been an increase in the incidence of asymptomatic hyperuricemia (AHU), which includes an increased level (>360 μmol/L) of uric acid (UA) in the blood serum of patients with no clinical manifestations of gout. AHU is reported in various rheumatic diseases, mainly in osteoarthritis, in which AHU is one of the manifestations of the metabolic syndrome. There is relationship between AHU and pulmonary hypertension in systemic sclerosis, arterial hypertension (AH) in men with seronegative rheumatoid arthritis, extensive cutaneous psoriasis and metabolic disorders in psoriatic arthritis. There are almost no data on AHU in ankylosing spondylitis (AS), which served objective for this work.Objective: to assess the association of AHU with AS duration and activity and the presence of comorbid diseases.Patients and methods. A retrospective analysis of 48 medical histories of patients with diagnosed AS, who were treated in V.A. Nasonova Research Institute of Rheumatology from 2015 to 2019 years, whose serum UA level was >360 μmol/L.Results and discussion. More than half of patients with AS and AHU were overweight, 21% were obese. AH was diagnosed in 43.7% of patients. Stage II–III chronic kidney disease was detected in 16.7% of patients, urolithiasis – in 18.8%. 4 (8.3%) patients had diabetes mellitus. The serum UA level in patients with AS was 422.0 ± 61.6 μmol/L. In patients with AS, an association between AHU and age, duration and disease activity was noted. There was no statistically significant rela- tionship between HG and blood glucose, cholesterol, creatinine levels, body mass index. Correlation analysis revealed a statistically significant relationship between the glomerular filtration rate (GFR) and the age of patients (r=-0.54, p<0.001), the duration of the disease (r=-0.40, p<0.05), cholesterol level (r=-0.48, p=0.01), UA level (r=-0.45, p=0.03) and blood pressure (r=-0.54, p<0.001). There was no association between disease activity and GFR (p>0.05).Conclusion. In AS, an association between an increased level of UA in the blood serum and the duration and activity of the disease, and patient's age, was established.

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Effect of the first and second postpartum partial milking on blood serum calcium concentration in dairy cows

Czech Journal of Animal Science ◽

10.17221/7292-cjas ◽

2014 ◽

Vol 59 (No. 3) ◽

pp. 128-133

Author(s):

E.G. Salgado-Hernández ◽

A. Aparicio-Cecilio ◽

F.H. Velásquez-Forero ◽

D.A. Castillo-Mata

Keyword(s):

Blood Serum ◽

Dairy Cows ◽

Serum Calcium ◽

Metabolic Disorders ◽

Calcium Concentration ◽

Atomic Absorption Spectrophotometry ◽

Serum Calcium Concentration ◽

Group 2 ◽

Group 1

Parturient paresis and subclinical hypocalcemia are frequent metabolic disorders in dairy cows postpartum. The aim of this study was to determine the effect of postpartum partial milking in the first two milkings on blood serum calcium concentration in dairy cows. Twenty multiparous Holstein dairy cows were randomized into two groups. Cows of group 1 (n = 10) were partially milked at the first and second milking postpartum. Cows of group 2 (n = 10) were completely milked. Blood samples were collected from all animals 5–7 days before calving, within 30 min after calving, and 4, 8, 12, 16, 20, 24, 28, and 32 h after calving for determination of serum calcium (Ca), phosphorus (P), and magnesium (Mg) concentrations. Colostrum production was registered and sampled in the first and second milking. Concentration of Ca in colostrum was determined by atomic absorption spectrophotometry. Serum Ca and P concentrations decreased in both groups after parturition (P < 0.05) and remained low during 32 h postpartum with no difference observed between groups (P > 0.05). Serum concentrations of Mg were stable in all samples and no statistical difference was observed between groups (P > 0.05). Colostrum production was higher in completely milked cows only in the first postpartum milking (P < 0.05), but there was no difference between groups at the second milking. Total Ca secretion in colostrum was higher in the complete milking group at the first and second postpartum milking. Colostrum Ca secretion increased at the second milking with respect to the first one in both groups (P < 0.05). There was no correlation between serum Ca and colostrum Ca (P > 0.05). In this study, the partial milking of colostrum in the first and second milking postpartum did not prevent subclinical hypocalcemia in dairy cows.  

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INDICES OF IMMUNE RESPONSES OF BULLS IN THE NECROBACILLOSIS VACCINATION OF THE CATTLE

Vestnik of Kazan state agrarin university ◽

10.12737/14777 ◽

2015 ◽

Vol 10 (3) ◽

pp. 93-97

Author(s):

Михеева ◽

Ekaterina Mikheeva ◽

Бабинцева ◽

Tatyana Babintseva ◽

Макаев ◽

...

Keyword(s):

T Lymphocytes ◽

Immune Responses ◽

Blood Serum ◽

B Lymphocytes ◽

Metabolic Disorders ◽

Specific Antibodies ◽

Biological Safety ◽

Digestive Organs ◽

Udmurt Republic ◽

And Control

Metabolic disorders and dysfunctional state of the digestive organs lead to the development of distal extremities diseases. The greatest danger is neсrobacrillosis. Vaccination is one of the ways to prevent and control this disease. The article reflects the data on the effect of vaccination (“Nekovak” and formol-emulsion neсrobacrillosis vaccine of Federal Centre for toxicology, radiation and biological safety) on immunological and serological parameters of blood and blood serum of cattle in the Udmurt Republic. After immunization with “Nekovak” vaccine and formol-emulsion neсrobacrillosis vaccine, we determined an increase of the number of leukocytes to 7th day, and also an increase of T-lymphocytes to 14th day. The number of B-lymphocytes reaches a maximum on 21st day. The content of gamma globulins, including specific antibodies against the neсrobacrillosis pathogen, exceeded the control and reached a maximum on the 21st day after vaccination.

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ROLE OF HUMAN DISKOIDIN DOMAIN RECEPTOR 1 IN THE PROGRESSION OF CHRONIC PYELONEPHRITIS IN CHILDREN

Ukrainian Journal of Nephrology and Dialysis ◽

10.31450/ukrjnd.2(38).2013.01 ◽

2017 ◽

pp. 5-20

Author(s):

N. N. Kaladze ◽

E. I. Slobodyan

Keyword(s):

Growth Factor ◽

Transforming Growth Factor ◽

Inverse Correlation ◽

Chronic Pyelonephritis ◽

Clinical Form ◽

Discoidin Domain Receptors ◽

Collagen Receptors ◽

Mediators Of Inflammation ◽

Tgf Β1

The purpose: to evaluate the role of collagen receptors Human Discoidin Domain Receptors (DDR1) as mediators of inflammation, proliferation and fibrosis in children with chronic pyelonephritis (CP), to reveal their relationship to the clinical form of the disease and the characteristics of its flow. Materials and methods: The levels of DDR1, transforming growth factor (TGF-β1), insulin-like growth factor (IGF-1) in the serum, β2 - microglobulin ( β2- MG) in the serum and urine were identified during the study of 40 children, ages 6 to 16 with CP in a state of clinical and laboratory remission. Results: Clinical and laboratory remission HP was associated with significant increased levels of DDR1 sera from long ill patients with frequent exacerbations, as well as 2-3 degree of activity last exacerbation, with family history. Found a strong inverse correlation between the levels of DDR1 and IGF-1, and the line with TGF-β1 and β2-MG of blood and urine. In patients with obstructive HP DDR1 level was significantly higher than in patients with non-obstructive clinical form. Conclusions: Increased serum DDR1 shows the progression of kidney damage with active fibrogenesis and inflammation in certain categories of patients with CP in a state of clinical and laboratory remission.

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Cardiotrophin-1 and components of metabolic syndrome: what is the role in the development of atrial fibrillation?

EP Europace ◽

10.1093/europace/euab116.131 ◽

2021 ◽

Vol 23 (Supplement_3) ◽

Author(s):

V Ionin ◽

EL Zaslavskaya ◽

EI Barashkova ◽

DS Skuridin ◽

EI Baranova ◽

...

Keyword(s):

Atrial Fibrillation ◽

Metabolic Syndrome ◽

Blood Serum ◽

Metabolic Disorders ◽

Type I Collagen ◽

Public Institution ◽

Type I ◽

Funding Sources ◽

Comparison Groups ◽

Energy Processes

Abstract Funding Acknowledgements Type of funding sources: Public Institution(s). Main funding source(s): Pavlov University Introduction. The cardiotrophin-1 (CT-1) is a cytokine from the interleukin-6 family associated with pathology of the cardiovascular system, contributing to myocardial remodeling and the development of fibrosis in patients with hypertension. CT-1 is involved in the regulation of energy processes and the metabolism of adipose tissue, as well as in the regulation of lipid and carbohydrate metabolism. Revealed an increase in the deposition of type I collagen in the atrial myocardium against the background of dilatation of the left atrium and an increase in the expression of CT-1. The role of CT-1 in the development of AF is currently being actively studied. Purpose. To determine the concentration of cardiotrophin-1 (CT-1) in patients with metabolic syndrome (MS) and atrial fibrillation (AF) with an assessment of the relationship with the parameters of obesity and metabolic disorders. Methods. In a one-stage case-control study, 352 patients aged 35 to 65 years were included: patients with MS (n = 223), of which 107 patients with AF, and comparison groups consisted of patients with AF without MS (n = 69) and practically healthy examined without cardiovascular diseases and metabolic disorders (n = 60). Results. It was found that the concentration of CT-1 in serum in patients with AF is higher than in patients with MS without AF (766,2 (539,2-1032,3) and 590,2 (480,1-922,3) pg/ml, p = 0,005) and higher than in healthy subjects (780,2 (550,2-1050,3) and 410,1 (290,2-549,2) pg/ml, p < 0,0001). It was found that in patients with AF and MS, the concentration of CT-1 in the blood serum is higher than in patients with AF without MS (851,2 (589,1-1146,3) and 681,1 (480,1-823,2) pg/ml, p = 0,004). CT-1 positively correlates with the concentration of glucose in blood plasma (r = 0,423, p = 0.002) and triglycerides (r = 0,207, p = 0,003), as well as with the waist circumference (r = 0,265, p < 0.001) and the thickness of epicardial fat (r = 0,351, p < 0,001). Using ROC analysis, it was found that with an increase in the concentration of CT-1 in blood serum more than 638,1 pg/ml, the probability of AF increased by 4 times (OR = 4.0, 95%CI 2,31-6,94, p < 0,0001). Conclusion. An increase of serum CT-1 concentration is associated with heart remodeling and obesity in patients with MS and probably has a pathogenetic role in increasing the risk of AF in this cohort of patients.

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Endogenic intoxication as a reflection of metabolic disorders in cows and possibilities of its pharmacological correction

BIO Web of Conferences ◽

10.1051/bioconf/20202700029 ◽

2020 ◽

Vol 27 ◽

pp. 00029

Author(s):

Marina P. Semenenko ◽

Tatyana A. Sakhno ◽

Elena V. Kuzminova ◽

Aleksey V. Savinkov

Keyword(s):

Blood Serum ◽

Metabolic Disorders ◽

Liver Cells ◽

Physiological Conditions ◽

Medium Mass ◽

Pharmacological Correction

The article presents the results of studies of medium mass molecules concentration in the blood serum of cows with different physiological conditions and correction of this state with livazen, which has an antioxidant and lipotropic effect on liver cells. It was determined that the use of livazen contributed to a decrease in cholesterol in the group of pregnant cows by 21.0 % and in the group of fresh cows by 15.9 %, with a decrease in the concentration of average molecular peptides by 4.0-11.8 % depending on the length of exposure.

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Acute Phase Proteins and Variables of Protein Metabolism in Dairy Cows during the Pre- and Postpartal Period

Acta Veterinaria Brno ◽

10.2754/avb200877010051 ◽

2008 ◽

Vol 77 (1) ◽

pp. 51-57 ◽

Cited By ~ 16

Author(s):

Cs. Tóthová ◽

O. Nagy ◽

H. Seidel ◽

J. Konvičná ◽

Z. Farkašová ◽

...

Keyword(s):

Blood Serum ◽

Dairy Cows ◽

Acute Phase ◽

Protein Metabolism ◽

Metabolic Disorders ◽

Serum Amyloid A ◽

Acute Phase Proteins ◽

Total Proteins ◽

Mean Values ◽

Amyloid A

The objective of the present study was to compare the concentrations of acute phase proteins and selected variables of protein metabolism in dairy cows of the Slovak Spotted breed from 4 weeks before parturition to 10 weeks after parturition. Acute phase proteins - haptoglobin (Hp) and serum amyloid A (SAA) - and variables of protein metabolism - total proteins, albumin, urea, creatinine, total immunoglobulins - were evaluated in blood serum. Significant differences were found in average values of the Hp and SAA concentrations in several groups during the monitored period (P < 0.05 and P < 0.001, respectively). The Hp and SAA concentrations in cows during the early postparturient period were significantly higher compared with the later postparturient period. Throughout the monitored time we found differences in mean values of total proteins (P < 0.001), urea (P < 0.001), and total immunoglobulins (P < 0.05). The concentrations of albumin decreased progressively in the postparturient period until 4 weeks after parturition. In the assessment of correlations between the monitored variables in the mentioned period we found significant correlations between Hp and SAA (r = 0.916; P < 0.001), total proteins and urea (r = 0.668; P < 0.05), total proteins and total immunoglobulins (r = 0.827; P < 0.01), and between total immunoglobulins and urea (r = 0.899; P < 0.001). The above mentioned results indicate that in the time around parturition there are significant changes in concentrations of acute phase proteins, as well as in the whole protein metabolism of dairy cows. These facts suggest that the postparturient period is a critical biological phase, throughout which there is the highest incidence of metabolic disorders.

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Features of fatty acid composition of blood serum in vibration disease

Russian Journal of Occupational Health and Industrial Ecology ◽

10.31089/1026-9428-2020-60-1-59-63 ◽

2020 ◽

pp. 59-63

Author(s):

I. A. Potapova

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Blood Serum ◽

Acid Composition ◽

Metabolic Disorders ◽

Clinical Manifestations ◽

Level Shift ◽

Vibration Disease ◽

Concentration Levels

Vibration disease (VD) is one of the most common occupational diseases. It is characterized by complex symptoms of the clinical picture. The study of the features of the course of VD of different severity from the position of metabolic disorders is an urgent issue, since it will allow to establish the most significant prognostic indicators in the future. Production vibration early enough leads to a shift in serum levels of higher fatty acids (HFA). This is largely due to the fact that HFA are structural units of lipids of cell membranes and precursors of biologically active mediators, are actively involved in the processes of energy supply. At present, there is no information on whether there is a reliable relationship between this indicator and the severity of clinical manifestations of VD.The aim of the study was to study the dependence of changes in the concentration levels of fatty acids, reflecting the specifics of metabolic disorders in VD, on the severity of pathological manifestations.The study of blood serum of workers of vibration-dangerous professions with vibration disease established in the clinic was carried out. The fatty acid composition of blood serum was studied using a gas chromatographic complex equipped with a flame ionization detector and a capillary column with a polyethylene glycol phase modified with nitroterephthalic acid. It is established that the complication of the clinical picture of vibration pathology on the background of authentic level shift s HFA: eicosatrienoic and arachidonic in males, γ-linolenic and eicosatrienoic in women. In addition, men tend to increase the levels of nervonic, eicosapentaenoic and docosahexaenoic acids with worsening of the degree of VD. Compared with workers exposed to vibration, but without pathological disorders, men have high levels of nervonic and docosahexaenoic, women-nervonic and palmitoleic acids.With varying degrees of severity of clinical manifestations of vibration pathology, certain changes in the metabolism of the HFA are observed. The nature of shift s in their concentration levels reflects the depth of violations of energy exchange reactions, synthesis of Pro-and anti-inflammatory mediators, membrane-pathological processes. The study of fatty-acid composition of blood serum in VD has prognostic value of detected level shift s and HFA can be estimated, what the metabolic process in the body of the examinee was subjected to the most grave violations, and to adjust the complex of therapeutic measures.Funding. The study had no funding.Conflict of interests. The authors declare no conflict of interests.

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Clinical and orthopedic examination of loose cows

Veterinaria Kubani ◽

10.33861/2071-8020-2020-5-14-17 ◽

2020 ◽

pp. 14-17

Author(s):

Aleksandr S. Krasnoperov ◽

◽

Aleksandr I. Belousov ◽

Larisa V. Khalturina ◽

Sergey V. Malkov ◽

...

Keyword(s):

Blood Serum ◽

Musculoskeletal System ◽

Dry Matter ◽

Metabolic Disorders ◽

Liver Enzymes ◽

Biochemical Study ◽

Clinical Signs ◽

Metabolic Characteristics ◽

Animal Feeding ◽

Agricultural Organization

The results of clinical and orthopedic examination of highly productive loose cows in the agricultural organization of Sverdlovsk region are presented in the article. Due to the high percentage of pathology of the musculoskeletal system in animals, this farm was selected. As a result of the study, violations of veterinary sanitary norms of maintenance and technological violations in animal feeding were found. On average, more than 40.0% of animals recorded clinical signs of pathology of the musculoskeletal system. Biochemical studies of urine and blood serum were performed to determine the metabolic characteristics of the blood of animals with orthopedic pathology. Hypostenuria was established in 41.7% of the examined cows according to the results of a biochemical study of urine. This indicates a lack of the required level of dry matter consumption. In 31.3% of cows, manifestations of metabolic disorders were recorded (a decrease in total protein and albumin, an increase in urea in the blood serum). In 25.0% of animals revealed the presence of intoxication syndrome - an increase in the activity of indicator liver enzymes (aspartate aminotransferase and glutamate dehydrogenase). Signs of development of osteodystrophic processes (a decrease in total calcium with an increase in alkaline phosphatase activity) were noted in more than 30.0% of cows.

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Age-related features of the manifestation of non-contagious pathology and metabolic disorders of liver in broiler chickens

BIO Web of Conferences ◽

10.1051/bioconf/20201700139 ◽

2020 ◽

Vol 17 ◽

pp. 00139

Author(s):

Marina P. Semenenko ◽

Elena V. Kuzminova ◽

Denis V. Osepchuk ◽

Vladimir A. Grin ◽

Ksenia A. Semenenko ◽

...

Keyword(s):

Blood Serum ◽

Broiler Chickens ◽

Metabolic Disorders ◽

Total Population ◽

Industrial Enterprise ◽

Liver Pathology ◽

Hepatic Parenchyma ◽

Age Related ◽

Causes Of Mortality

The article presents the data on the study of age-related features of non-contagious pathology in broiler chickens, as well as the level of metabolic processes in liver of healthy poultry and poultry with diseases. The results of the studies indicate that in the conditions of an industrial enterprise specializing in meat poultry rearing, poultry mortality in different age periods ranged from 3 to 5 % of the total population. In the structure of the causes of mortality of broiler chickens from the 8th to the 15th day, an increase in mortality from hepatopathies was registered, and by the 25th day of life hepatosis was recorded in the bulk of the dead poultry. Biochemical monitoring of blood serum in poultry with signs of liver pathology revealed an increase in the level of ALAT, β-globulins, and the “inflammation syndrome” of the hepatic parenchyma was confirmed by a positive thymol test. Long-term consumption of feed contaminated with mycotoxins by broilers led to the deterioration in the health of the poultry, an imbalance in liver and the development of general metabolic disorders of the poultry organism.

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