scholarly journals Sivas İlinde Kültürü Yapılan Kenger (Gundellia tournefortii L.) Bitkisi Tohumlarının Besin Elementlerinin ve Biyolojik Aktivitelerinin Değerlendirilmesi

2019 ◽  
Vol 7 (sp2) ◽  
pp. 52
Author(s):  
Handan Saraç ◽  
Ahmet Demirbaş ◽  
Sevgi Durna Daştan ◽  
Mehmet Ataş ◽  
Özge Çevik ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Water Extract ◽  
Mass Spectrometry Analysis ◽  
Stress Index ◽  
Aureus Strain ◽  
Klebsiella Pneumonia ◽  
Oxidative Stress Index

The aim of the present study is to conduct a general content evaluation of water extract obtained from the seeds of Gundellia tournefortii by GC-MS (Gas chromatography–Mass spectrometry) analysis, to determine its macro and micro element concentrations, antimicrobial activity and total antioxidant level (TAS), total oxidant level (TOS), oxidative stress index (OSI) values, and to reveal its anti-carcinogenic properties on various cell lines. Rel Assay Diagnostics kits were used to determine TAS, TOS and OSI values. By determining the minimum inhibition concentration (MIC) value by Microdilution Broth method, antimicrobial activity analyses were performed on microorganisms which are Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Bacillus cereus (ATCC11778), Klebsiella pneumonia (ATCC 13883), Candida albicans (ATCC 10231) and Candida tropicalis (DSM11953), respectively. By MTT method, cytotoxic activity was determined on normal mouse fibroblast cell line, HUVEC and 3 different human cancer cell lines. As a result, it was determined that Gundelia tournefortii plant extract has a rather weak antimicrobial activity except for on S. aureus strain, a good antioxidant activity, and a cytotoxic activity in some cells. As for the oxidative stress index of the plant, it was found to be low. In addition, in terms of macro and micro nutrient content of the plant Gundellia tournefortii has concentrations of 3.64% N, 0.11% P, 3.78% K, 0.22% Ca, 0.57% Mg, 268.4 mg/kg Fe, 16.7 mg/kg Zn, 19.4 mg/kg Mn and 8.3 mg/kg Cu.

scholarly journals Önemli Tıbbi Bitkilerden Biri Olan Achillea millefolium L. (Civanperçemi) Bitkisinin Besin Elementi Konsantrasyonları ve Antioksidan Aktivitesi

2021 ◽  
Vol 9 (3) ◽  
pp. 590-594
Author(s):  
Handan Saraç ◽  
Hasan Durukan ◽  
Ahmet Demirbaş
Keyword(s):  
Oxidative Stress ◽  
Water Extract ◽  
Total Antioxidant Status ◽  
Stress Index ◽  
Achillea Millefolium ◽  
Nutrient Concentrations ◽  
Oxidative Stress Index ◽  
Total Antioxidant ◽  
Oxidant Status ◽  
And Oxidative Stress

In this study, it was aimed to determine the nutrient concentrations and antioxidant activity of Achillea millefolium L. (Yarrow), which is known to have medicinal properties. For this purpose, macro and micronutrient concentrations, total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of water extract Yarrow plant, which is known to be collected from Divrigi district in Sivas province, were determined. The results have shown that the yarrow plant is sufficient in terms of some macro and micronutrient concentrations, and the nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium from macro elements, iron (Fe), zinc (Zn), manganese (Mn) and copper (Cu) from micro elements concentrations were determined as 1.01% N, 0.63% P, 2.43% K, 2.22% Ca, 0.70% Mg, 360.4 mg kg-1 Fe, 47.6 mg kg-1 Zn, 85.5 mg kg-1 Mn and 28.3 mg kg-1 Cu, respectively. It has been determined that its antioxidant potential is moderate. In addition, the plant’s oxidant capacity and oxidative stress index were found to be low.

scholarly journals Sivas İlinde Doğal Olarak Yetişen ve Yenilebilir Bir Bitki Olan Evelik (Rumex crispus) Bitkisinin Biyoaktivitesi ve Terapotik Nitelikleri

2019 ◽  
Vol 7 (sp2) ◽  
pp. 67
Author(s):  
Sevgi Durna Daştan ◽  
Hasan Durukan ◽  
Ahmet Demirbaş ◽  
Erol Dönmez
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Plant Extracts ◽  
Human Cancer ◽  
Biological Effects ◽  
Scientific Data ◽  
Stress Index ◽  
Klebsiella Pneumonia ◽  
Rumex Crispus

Although scientific data on the biological effects and mechanisms action of many extracts from plants are still insufficient, interest in this issue is increasing day by day. In this study, Rumex crispus (Evelik) was collected from Sivas region during the season and dried, and then ethanol, methanol and water extractions were made from leafy parts. The aim of the study was to evaluate the antimicrobial activities of each extract on some microorganisms, total antioxidant level (TAS), total oxidant level (TOS), oxidative stress index (OSI) values and anticarcinogenic properties on various cell lines. Rel Assay Diagnostics kits were used to determine TAS, TOS and OSI values. Microdilution broth method was used to determine the minimum inhibition concentration (MIC) of plant extracts against microorganisms. Antimicrobial activity assays in the study were used Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Bacillus cereus (ATCC 11778), Klebsiella pneumonia (ATCC 13783) Candida albicans (10231) and Candida tropicalis (DSM11953). Cytotoxic activity of plant extracts was determined by MTT method in normal Mouse fibroblast cell line and 3 different human cancer cell line. As a result, it was found that ethanol, methanol and water extracts of domestic plants do not have antimicrobial activity on microorganisms we tested, they have high antioxidant activity and have cytotoxic activity in some cell cultures.

scholarly journals Total Antioxidant Capacity and Lipid Peroxidation Status in Cervical Cancer Patients Compared with Women Without Cervical Cancer in Bangladesh

2021 ◽  
Vol 19 (4) ◽  
Author(s):  
Taslima Nigar ◽  
Annekathryn Goodman ◽  
Shahana Pervin
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Cancer Patients ◽  
Total Antioxidant Capacity ◽  
Stress Index ◽  
Oxidative Stress Index ◽  
Total Antioxidant

Abstract Purpose Over the past several decades, research has suggested reactive oxygen species act as cofactors for cervical cancer development. The aim of this study is to evaluate the antioxidant and lipid peroxidation status in cervical cancer patients in Bangladesh. Methods From December 2017 to 2018, a cross-sectional observational study was conducted on 50 cervical cancer patients and 50 controls. Plasma levels of lipid peroxidation and total antioxidant capacity were measured. The Student’s t test was used for statistical analysis. P values less than 0.05 were taken as a level of significance. Results There was a significant reduction in total antioxidant levels in patients with cervical cancer, 972.77 ± 244.22 SD µmol equivalent to ascorbic acid/L, compared to normal controls, 1720.13 ± 150.81 SD µmol equivalent to ascorbic acid/L (P < 0.001). Levels of lipid peroxidation were found to be significantly higher in cervical cancer, 7.49 ± 2.13 SD µmol/L, than in women without cervical cancer, 3.28 ± 0.58 SD µmol/L (P < 0.001). The cervical cancer patients had significantly higher levels of oxidative stress index (0.83 ± 0.31) in comparison to controls (0.19 ± 0.04) (P < 0.001). Conclusion There was an increased oxidative stress index due to imbalance between lipid peroxidation generation and total antioxidant capacity in cervical cancer patients. Further studies are needed to explore the role of oxidative stress as a cofactor for cervical carcinogenesis.

scholarly journals Melatonin attenuates ovarian ischemia reperfusion injury in rats by decreasing oxidative stress index and peroxynitrite

2020 ◽  
Vol 50 (6) ◽  
pp. 1513-1522
Author(s):  
Şenol KALYONCU ◽  
Bülent YILMAZ ◽  
Mustafa DEMİR ◽  
Meltem TUNCER ◽  
Zehra BOZDAĞ ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Stress Index ◽  
Oxidative Stress Index ◽  
Group 6 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Background/aim: To evaluate the protective effect of melatonin on ovarian ischemia reperfusion injury in a rat model. Materials and methods: Forty-eight rats were separated equally into 6 groups. Group 1: sham; Group 2: surgical control with 3-h bilateral ovarian torsion and detorsion; Group 3: intraperitoneal 5% ethanol (1 mL) just after detorsion (as melatonin was dissolved in ethanol); Group 4: 10 mg/kg intraperitoneal melatonin 30 min before 3-h torsion; Group 5:10 mg/kg intraperitoneal melatonin just after detorsion; Group 6:10 mg/kg intraperitoneal melatonin 30 min before torsion and just after detorsion. Both ovaries and blood samples were obtained 7 days after detorsion for histopathological and biochemical analysis.Results: In Group 1, serum levels of total oxidant status (TOS) (μmol H2O2 equivalent/g wet tissue)were significantly lower than in Group2 (P = 0.0023), while tissue TOS levels were lower than in Group 3 (P = 0.0030). Similarly, serum and tissue levels of peroxynitrite in Group 6were significantly lower than those ofGroup 2 (P = 0.0023 and P = 0.040, respectively). Moreover, serum oxidative stress index (OSI) (arbitrary unit) levels were significantly increased in Group 2 when compared to groups 1 and 6 (P = 0.0023 and P= 0.0016, respectively) and in Group 3 with respect to groups 1, 4, 5, and 6 (P = 0.0023, P = 0.0026, P = 0.0008, and P = 0.0011, respectively). Furthermore, there was a significant decrease in histopathological scores including follicular degeneration, vascular congestion, hemorrhage, and inflammation in the melatonin and sham groups in comparison with control groups. Additionally, primordial follicle count was significantly higher in Group 6 than in Group 2 (P = 0.0002).Conclusion: Melatonin attenuates ischemia reperfusion damage in a rat torsion/detorsion model by improving histopathological and biochemical findings including OSI and peroxynitrite.

Balanced oxidative stress index in spite of decreased uric acid levels in multiple sclerosis patients

2015 ◽  
Vol 9 (2) ◽  
pp. 153-158 ◽  
Author(s):  
O. Aydin ◽  
F. Kurtulus ◽  
E. Eren ◽  
H. Y. Ellidag ◽  
N. Yılmaz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Multiple Sclerosis ◽  
Uric Acid ◽  
Stress Index ◽  
Oxidative Stress Index ◽  
Multiple Sclerosis Patients

The Association Between Alterations in Redox Homeostasis, Cortisol, and Commonly Used Objective and Subjective Markers of Fatigue in American Collegiate Football

2021 ◽  
pp. 1-7
Author(s):  
Benjamin A. McKay ◽  
Jace A. Delaney ◽  
Andrew Simpkin ◽  
Theresa Larkin ◽  
Andrew Murray ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
College Football ◽  
Oxygen Radical ◽  
Flight Time ◽  
Stress Index ◽  
Strength Index ◽  
Urinary Cortisol ◽  
Free Oxygen Radical ◽  
Free Oxygen ◽  
Oxidative Stress Index

Purpose: To assess associations between a free oxygen radical test (FORT), free oxygen radical defense test (FORD), oxidative stress index, urinary cortisol, countermovement jump (CMJ), and subjective wellness in American college football. Methods: Twenty-three male student athlete American college football players were assessed over 10 weeks: off-season conditioning (3 wk), preseason camp (4 wk), and in season (3 wk). Assessments included a once-weekly FORT and FORD blood sample, urinary cortisol sample, CMJ assessment including flight time, reactive strength index modified and concentric impulse, and a daily subjective wellness questionnaire. Linear mixed models analyzed the effect of a 2 within-subject SD change in the predictor variable on the dependent variable. The effects were interpreted using magnitude-based inference and are presented as standardized effect size (ES) ± 90% confidence intervals. Results: Small negative associations were observed between FORT–flight time, FORT–fatigue, FORT–soreness (ES range = −0.30 to −0.48), FORD–sleep (ES = 0.42 ± 0.29), and oxidative stress index soreness (ES = 0.56 ± 0.29). Small positive associations were observed between FORT–cortisol (ES = 0.36 ± 0.35), FORD–flight time, FORD reactive strength index modified and FORD–soreness (0.37–0.41), oxidative stress index concentric impulse (ES = 0.37 ± 0.28), and with soreness–concentric impulse, soreness–flight time, and soreness reactive strength index modified (0.33–0.59). Moderate positive associations were observed between cortisol–concentric impulse and cortisol–sleep (0.57–0.60). Conclusion: FORT/FORD was associated with CMJ variables and subjective wellness. Greater amounts of subjective soreness were associated with decreased CMJ performance, increased FORT and cortisol, and decreased FORD.

Evaluation of Dynamic Thiol-Disulfide Balance in Preinvasive Lesions of the Cervix

2021 ◽  
Author(s):  
Burak Sezgin ◽  
Fatih Pirinççi ◽  
Aysun Camuzcuoğlu ◽  
Eda Adeviye Şahin ◽  
Özcan Erel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Papillomavirus ◽  
Stress Index ◽  
Lesion Group ◽  
Control Group ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Oxidative Stress Index ◽  
Preinvasive Lesions ◽  
Intraepithelial Lesion

Abstract Purpose: This study aimed to determine the potential clinical use of dynamic thiol disulfide balance in cases with preinvasive lesions of the cervix.Methods: One hundred and sixteen patients with high-grade squamous intraepithelial lesion, one hundred patients with low-grade squamous intraepithelial lesion and one hundred and ten healthy controls were enrolled in the study. A fully automated colorimetric system was used to determine the levels of thiol-disulfide parameters. The ischemia-modified albumin, total oxidant-antioxidant capacity, oxidative stress index of the retrieved cases were further analysed.Results: Native thiol and total thiol levels are significantly lower in the high-grade squamous intraepithelial lesion group according to control group (p:0.004 and p:0.015, respectively). Disulfide level is significantly increased in the high-grade squamous intraepithelial lesion group compared to control group (p:0.004). Oxidative stress index levels in high-grade squamous intraepithelial lesion group were observed as significantly higher according to the control group (p:0.014). Ischemia-modified albumin levels in the high-grade squamous intraepithelial lesion group were observed as significantly higher compared to the control group (p:0.020). Disulfide levels are positively correlated with risk type of Human papillomavirus (r:0.420, p<0.001).Conclusion: The analysis of dynamic thiol disulfide balance revealed considerable oxidative damage in patients with Human papillomavirus -related cervical precursor lesions compared to women with ordinary cytology specimens. Therefore, investigation of thiol disulfide balance with presented method represents a new promising test for early diagnosis and management of women at high risk for cervical cancer.

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