scholarly journals VIABILITAS SEL DAN AKTIVITAS ANTIMIKROBA BIO-KAPSUL PROBIOTIK Lb paracasei ssp paracasei ML3 HASIL EKSTRUSI KARAGENAN-SKIM (CELL VIABILITY AND ANTIMICROBIAL ACTIVITY OF PROBIOTIC BIO-CAPSULE Lb paracasei ssp paracasei ML3 by EXTRUSION KARAGENAN-SKIM)

2020 ◽  
Vol 20 (3) ◽  
Author(s):  
Mutia Elida ◽  
Gusmalini Gusmalini ◽  
Agustina Agustina ◽  
Iza Ayu Saufani
Keyword(s):  
Antimicrobial Activity ◽  
Cell Viability ◽  
Skim Milk ◽  
Coating Material ◽  
Lactobacillus Paracasei ◽  
Bacterial Cells ◽  
Coating Materials ◽  
Coating Agent ◽  
Extrusion Method

Production of Lactobacillus paracasei ssp paracasei Ml3 bio-capsules using a coating agent of carrageenan and skim milk as a protective and stabilizer for the viability of encapsulated bacterial cells during processing. This research was carried out to examine the viability of Lactobacillus paracasei ssp paracasei Ml3 and the antimicrobial activity against Eschericia coli in wet and dry bio-capsules after contact 3 hour. Production of bio-capsules uses extrusion method, with the ratio of coating materials for carrageenan and skim milk, namely 1: 1, 2: 1, and 3: 1.  Each treatment was repeated 3 times. The result showed that the coating material (carrageenan: skim milk) with 2:1 ratio in wet dan dry bio-capsules were able to maintain the viability of Lactobacillus paracasei ssp paracasei Ml3 and only decreased 0.72 log cfu/g and 1.31 log cfu/g, respectively. Antimicrobial activity was indicated  by the highest decrease number of Eschericia coli after contact 3 hour with bio-capsules 2:1 , namely 2.21 log cfu/g and 2.15 log cfu/g, respectively.

scholarly journals VIABILITY AND SURVIVAL RATE OF PROBIOTIC LACTOBACILLUS PARACASEI SSP. PARACASEI MI3 IN CARRAGEENAN-SKIM MILK BIO-CAPSULES

2020 ◽  
Vol 8 (12) ◽  
pp. 289-293
Author(s):  
M. Elida ◽  
◽  
Gusmalini Gusmalini ◽  
I.A. Saufani ◽  
◽  
...  
Keyword(s):  
Survival Rate ◽  
Cell Viability ◽  
Production Process ◽  
Gastric Acid ◽  
Negative Impact ◽  
Skim Milk ◽  
Low Ph ◽  
Lactobacillus Paracasei ◽  
Probiotic Lactobacillus

The ingredients of coated carrageenan which mixed with skim milk as the physical immersion material will able to stabilize the cell viability along the production process. It could reduce the negative impact of the processes, and decrased of resistance inside gastrointestinal due to the release of gastric acid. This study aimed to compare of Carrageenan-skim against bio-capsule viability, probiotic survival, bio-capsule viability at low pH 2.0 and the synthesis of probiotics in bio-capsules pH 2.0. The microencapsulation procedure with extrusion technique were performed with different composition of coated ingredients and dropped into a sterile 3% KCL solution. The respective composition are 1:1, 2:1 and 3:1.Carrageenan-skim coating can significantly protect the probiotic cells (P ≤ 0.05) with high visibility and durability of the pH 2.0 as well as bio-capsule probiotic synthesis and bio-capsule synthesis at pH 2.0 in various comparisons. The comparison of 2:1 was obtained the highest viability of 1.97 x 109 CFU/G and suffered a decrease in visibility during extrusion of 0.7 Log CFU/G, with a synthesis in bio-capsules of 93.3%. Resistance to pH 2.0 after 3 hours incubation is 2.6 x 108 with a decrease after extrusion 0.89 Log CFU/G and the survival rate in bio-capsule pH 2.0 is 82.94%.

Mechanisms involved in effects of antimicrobial peptides, bactenectins ChBac3.4, ChBac5, and mini-ChBac7.5Nb, on bacterial cells

2017 ◽  
pp. 43-50
Author(s):  
О.В. Шамова ◽  
М.С. Жаркова ◽  
П.М. Копейкин ◽  
Д.С. Орлов ◽  
Е.А. Корнева
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Innate Immunity ◽  
Infectious Diseases ◽  
Metabolic Activity ◽  
Capra Hircus ◽  
Bacterial Cells ◽  
Antibiotic Resistant ◽  
Resistant Strains

Антимикробные пептиды (АМП) системы врожденного иммунитета - соединения, играющие важную роль в патогенезе инфекционных заболеваний, так как обладают свойством инактивировать широкий спектр патогенных бактерий, обеспечивая противомикробную защиту живых организмов. В настоящее время АМП рассматриваются как потенциальные соединения-корректоры инфекционной патологии, вызываемой антибиотикорезистентными бактериями (АБР). Цель данной работы состояла в изученим механизмов антибактериального действия трех пептидов, принадлежащих к семейству бактенецинов - ChBac3.4, ChBac5 и mini-ChBac7.5Nb. Эти химически синтезированные пептиды являются аналогами природных пролин-богатых АМП, обнаруженных в лейкоцитах домашней козы Capra hircus и проявляющих высокую антимикробную активность, в том числе и в отношении грамотрицательных АБР. Методы. Минимальные ингибирующие и минимальные бактерицидные концентрации пептидов (МИК и МБК) определяли методом серийных разведений в жидкой питательной среде с последующим высевом на плотную питательную среду. Эффекты пептидов на проницаемость цитоплазматической мембраны бактерий для хромогенного маркера исследовали с использованием генетически модифицированного штамма Escherichia coli ML35p. Действие бактенецинов на метаболическую активность бактерий изучали с применением маркера резазурина. Результаты. Показано, что все исследованные пептиды проявляют высокую антимикробную активность в отношении Escherichia coli ML35p и антибиотикоустойчивых штаммов Escherichia coli ESBL и Acinetobacter baumannii in vitro, но их действие на бактериальные клетки разное. Использован комплекс методик, позволяющих наблюдать в режиме реального времени динамику действия бактенецинов в различных концентрациях (включая их МИК и МБК) на барьерную функцию цитоплазматической мембраны и на интенсивность метаболизма бактериальных клеток, что дало возможность выявить различия в характере воздействия бактенецинов, отличающихся по структуре молекулы, на исследуемые микроорганизмы. Установлено, что действие каждого из трех исследованных бактенецинов в бактерицидных концентрациях отличается по эффективности нарушения целостности бактериальных мембран и в скорости подавления метаболизма клеток. Заключение. Полученная информация дополнит существующие фундаментальные представления о механизмах действия пролин-богатых пептидов врожденного иммунитета, а также послужит основой для биотехнологических исследований, направленных на разработку на базе этих соединений новых антибиотических препаратов для коррекции инфекционных заболеваний, вызываемых АБР и являющимися причинами тяжелых внутрибольничных инфекций. Antimicrobial peptides (AMPs) of the innate immunity are compounds that play an important role in pathogenesis of infectious diseases due to their ability to inactivate a broad array of pathogenic bacteria, thereby providing anti-microbial host defense. AMPs are currently considered promising compounds for treatment of infectious diseases caused by antibiotic-resistant bacteria. The aim of this study was to investigate molecular mechanisms of the antibacterial action of three peptides from the bactenecin family, ChBac3.4, ChBac5, and mini-ChBac7.5Nb. These chemically synthesized peptides are analogues of natural proline-rich AMPs previously discovered by the authors of the present study in leukocytes of the domestic goat, Capra hircus. These peptides exhibit a high antimicrobial activity, in particular, against antibiotic-resistant gram-negative bacteria. Methods. Minimum inhibitory and minimum bactericidal concentrations of the peptides (MIC and MBC) were determined using the broth microdilution assay followed by subculturing on agar plates. Effects of the AMPs on bacterial cytoplasmic membrane permeability for a chromogenic marker were explored using a genetically modified strain, Escherichia coli ML35p. The effect of bactenecins on bacterial metabolic activity was studied using a resazurin marker. Results. All the studied peptides showed a high in vitro antimicrobial activity against Escherichia coli ML35p and antibiotic-resistant strains, Escherichia coli ESBL and Acinetobacter baumannii, but differed in features of their action on bacterial cells. The used combination of techniques allowed the real-time monitoring of effects of bactenecin at different concentrations (including their MIC and MBC) on the cell membrane barrier function and metabolic activity of bacteria. The differences in effects of these three structurally different bactenecins on the studied microorganisms implied that these peptides at bactericidal concentrations differed in their capability for disintegrating bacterial cell membranes and rate of inhibiting bacterial metabolism. Conclusion. The obtained information will supplement the existing basic concepts on mechanisms involved in effects of proline-rich peptides of the innate immunity. This information will also stimulate biotechnological research aimed at development of new antibiotics for treatment of infectious diseases, such as severe in-hospital infections, caused by antibiotic-resistant strains.

Refined palm oil fractions: Effect of skim milk powder and maltodextrin on emulsion properties and microencapsulation by spray drying

2021 ◽  
Author(s):  
K. Cevik ◽  
H. Yalcin
Keyword(s):  
Spray Drying ◽  
Palm Oil ◽  
Palm Olein ◽  
Milk Powder ◽  
Skim Milk ◽  
Palm Stearin ◽  
Skim Milk Powder ◽  
Solid Content ◽  
Coating Materials ◽  
Oil Fractions

Abstract The aim of this study was to investigate microencapsulation of palm oil fractions (palm olein (POL) and 90% palm olein+10% palm stearin (POS)) using skim milk powder (SMP) and maltodextrin (MD) by spray drying. Twenty-seven emulsions with POL were prepared to determine appropriate solid content (SC) and oil/coating material ratio (O/CM) of the emulsions to be fed into the spray dryer. Emulsion properties, such as viscosity and stability, were affected by SC and coating materials. The effects of coating materials used in microencapsulation of POL and POS were also tested by using different ratios of SMP and MD. The microencapsulation efficiency (69.28–84.97%), the microencapsulation yield (14.50–31.79%), and the peroxide value (4.12–7.07 meq O2/kg oil) of the powders were affected by the coating materials (P < 0.05).

scholarly journals Anticancer and Antimicrobial Activity Evaluation of Cowpea-Porous-Starch-Formulated Silver Nanoparticles

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Shiara Ramdath ◽  
John Mellem ◽  
Londiwe Simphiwe Mbatha
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Cell Viability ◽  
High Dose ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Anticancer Drug Delivery ◽  
Ic50 Values ◽  
Biosynthesized Agnps

Health issues involving inadequate treatment of diseases such as cancer and microbial infections continue to be the subject of much ongoing recent research. Biosynthesized silver nanoparticles (AgNPs) were characterized using Transmission Electron Microscopy (TEM), Zeta Sizer, Ultraviolet (UV), and Fourier Transform Infrared (FTIR) spectroscopy. Their antimicrobial activity was evaluated on selected Gram-positive and Gram-negative bacterial strains, using the disc diffusion and broth dilution assays. Cell viability profiles were evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and apoptosis studies on selected human noncancer and cancer cells. The biosynthesized AgNPs were evaluated to be spherical clusters, with sizes between 40 and 70 nm. The absorption peak at 423 nm and the presence of polyphenols confirmed the synthesis and stabilization of these tested AgNPs. The AgNPs showed a good stability of −23.9 ± 1.02 mV. Good antimicrobial activity (6.0–18.0 mm) was seen on all tested bacteria at a minimum inhibitory concentration (MIC) ranging from 5 to 16 μg/ml, with the highest activity seen against Gram-negative Escherichia coli (18 ± 0.5 mm), and the lowest activity was seen against Gram-positive Listeria monocytogenes (6.0 ± 0.4 mm) after treatment with the AgNPs. These NPs showed a concentration-dependent and cell-specific cytotoxicity with low IC50 values (41.7, 56.3, and 63.8 μg/ml). The NPs were well tolerated by tested cells as indicated by a more than 50% cell viability at the high dose tested and low apoptotic indices (<0.2). These findings indicated that these biosynthesized AgNPs showed great potential as effective antibacterial agents and anticancer drug delivery modalities.

scholarly journals Low-Power Red and Infrared Laser Effects on Cells Deficient in DNA Repair

2019 ◽  
Vol 10 (3) ◽  
pp. 157-162
Author(s):  
Lucas Kiyoshi da Fonseca Iwahara ◽  
Flavia de Paoli ◽  
Adenilson de Souza da Fonseca
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Low Power ◽  
Cell Viability ◽  
Bacterial Cells ◽  
Wild Type ◽  
Low Level ◽  
Affect Cell Viability ◽  
Study Results ◽  
Emission Mode

Introduction: Low-level lasers are successfully used to prevent and treat diseases in soft oral and bone tissues, particularly diseases in oral cavity caused by chemotherapy and radiotherapy in oncology. However, controversy exists as to whether these lasers induce molecular side effects, mainly on DNA. The aim of this work was to assess the effects of low-power lasers on mutant Escherichia coli cells in DNA repair. Methods: Escherichia coli wild type cultures as well as those lacking recombination DNA repair (recA- ) and la SOS responses (lexA- ) irradiated with lasers at different energy densities, powers, and emission modes for cell viability and morphology assessment were used in this study. Results: Laser irradiation: (i) did not affect cell viability of non-mutant and lexA- cells but decreased viability in recA- cultures; (ii) altered morphology of wild type and lexA, depending on the energy density, power, emission mode, and wavelength. Conclusion: Results show that low-level lasers have lethal effects on both recombination DNA repair and SOS response bacterial cells but do not induce morphological modifications in these cells.

Study of the System Wood – Coating Material. I. Wood – Liquid Coating Material

Holzforschung ◽  
2000 ◽  
Vol 54 (2) ◽  
pp. 189-196 ◽  
Author(s):  
Eva Liptáková ◽  
Jozef Kúdela ◽  
Ján Sarva
Keyword(s):  
Phase Boundary ◽  
Rheological Properties ◽  
Wood Surface ◽  
Surface Forces ◽  
Coating Material ◽  
Actual State ◽  
Wood Coating ◽  
Coating Materials ◽  
Wetting Process ◽  
Adhesion Work

Summary This paper deals with the evaluation of wood—wetting process with coating materials on the basis of interactions of surface forces on phase boundary. The obtained results are compared with the actual state in the system wood—coating material. Primary ability of coating materials—spontaneous spreading over the wood surface has been proved. There is also the secondary influence of rheological properties of coating materials causing deformations of the phase boundary, non-perfect wetting of the wood surface and apparent lowering of adhesion work. The influence of rheological properties has been expressed by using the coefficient F the meaning of which follows from the comparison between results of adhesion work computed according to modified Young-Dupré equation and of adhesion work determined on the basis of the interactions of surface forces on the phase boundary between wood and liquid coating materials. A direct dependence between the values of the coefficient F and coating materials viscosity has been proved.

scholarly journals The Gut Microbiota and Human Health with an Emphasis on the Use of Microencapsulated Bacterial Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Satya Prakash ◽  
Catherine Tomaro-Duchesneau ◽  
Shyamali Saha ◽  
Arielle Cantor
Keyword(s):  
Gastrointestinal Tract ◽  
Gut Microbiota ◽  
Cell Viability ◽  
Crucial Role ◽  
Bacterial Population ◽  
Significant Loss ◽  
Bacterial Cells ◽  
Lower Gastrointestinal Tract ◽  
Viable Cells ◽  
Harsh Conditions

The gut microbiota plays a crucial role in maintaining health. Alterations of the gut bacterial population have been associated with a number of diseases. Past and recent studies suggest that one can positively modify the contents of the gut microbiota by introducing prebiotics, probiotics, synbiotics, and other therapeutics. This paper focuses on probiotic modulation of the gut microbiota by their delivery to the lower gastrointestinal tract (GIT). There are numerous obstacles to overcome before microorganisms can be utilized as therapeutics. One important limitation is the delivery of viable cells to the lower GIT without a significant loss of cell viability and metabolic features through the harsh conditions of the upper GIT. Microencapsulation has been shown to overcome this, with various types of microcapsules available for resolving this limitation. This paper discusses the gut microbiota and its role in disease, with a focus on microencapsulated probiotics and their potentials and limitations.

Influence of Carvacrol and 1,8-Cineole on Cell Viability, Membrane Integrity, and Morphology of Aeromonas hydrophila Cultivated in a Vegetable-Based Broth

2015 ◽  
Vol 78 (2) ◽  
pp. 424-429 ◽  
Author(s):  
JOSSANA PEREIRA de SOUSA ◽  
KATARYNE ÁRABE RIMÁ de OLIVEIRA ◽  
REGINA CELIA BRESSAN QUEIROZ de FIGUEIREDO ◽  
EVANDRO LEITE de SOUZA
Keyword(s):  
Cell Viability ◽  
Membrane Permeability ◽  
Aeromonas Hydrophila ◽  
Membrane Integrity ◽  
Cell Membrane Permeability ◽  
Confocal Laser ◽  
Wall Structure ◽  
Bacterial Cells ◽  
Transmission Electron ◽  
Cytoplasmic Content

This study investigated the effects of carvacrol (CAR) and 1,8-cineole (CIN) alone (at the MIC) or in combination at subinhibitory amounts (both at 1/8 MIC) on the cell viability, membrane permeability, and morphology of Aeromonas hydrophila INCQS 7966 (A. hydrophila) cultivated in a vegetable-based broth. CAR and CIN alone or in combination severely affected the viability of the bacteria and caused dramatic changes in the cell membrane permeability, leading to cell death, as observed by confocal laser microscopy. Scanning and transmission electron microscopy images of bacterial cells exposed to CAR or CIN or the mixture of both compounds revealed severe changes in cell wall structure, rupture of the plasma membrane, shrinking of cells, condensation of cytoplasmic content, leakage of intracellular material, and cell collapse. These findings suggest that CAR and CIN alone or in combination at subinhibitory amounts could be applied to inhibit the growth of A. hydrophila in foods, particularly as sanitizing agents in vegetables.

scholarly journals Coating Matters: Review on Colloidal Stability of Nanoparticles with Biocompatible Coatings in Biological Media, Living Cells and Organisms

2018 ◽  
Vol 25 (35) ◽  
pp. 4553-4586 ◽  
Author(s):  
Jonas Schubert ◽  
Munish Chanana
Keyword(s):  
Colloidal Stability ◽  
Biological Fluids ◽  
Chemical Properties ◽  
Coating Material ◽  
Living Systems ◽  
Biological Media ◽  
Coating Materials ◽  
Physico Chemical ◽  
Environment Temperature ◽  
To Come

Within the last two decades, the field of nanomedicine has not developed as successfully as has widely been hoped for. The main reason for this is the immense complexity of the biological systems, including the physico-chemical properties of the biological fluids as well as the biochemistry and the physiology of living systems. The nanoparticles’ physicochemical properties are also highly important. These differ profoundly from those of freshly synthesized particles when applied in biological/living systems as recent research in this field reveals. The physico-chemical properties of nanoparticles are predefined by their structural and functional design (core and coating material) and are highly affected by their interaction with the environment (temperature, pH, salt, proteins, cells). Since the coating material is the first part of the particle to come in contact with the environment, it does not only provide biocompatibility, but also defines the behavior (e.g. colloidal stability) and the fate (degradation, excretion, accumulation) of nanoparticles in the living systems. Hence, the coating matters, particularly for a nanoparticle system for biomedical applications, which has to fulfill its task in the complex environment of biological fluids, cells and organisms. In this review, we evaluate the performance of different coating materials for nanoparticles concerning their ability to provide colloidal stability in biological media and living systems.

scholarly journals Evaporation-induced stimulation of bacterial osmoregulation for electrical assessment of cell viability

2016 ◽  
Vol 113 (26) ◽  
pp. 7059-7064 ◽  
Author(s):  
Aida Ebrahimi ◽  
Muhammad Ashraful Alam
Keyword(s):  
Cell Viability ◽  
Osmotic Pressure ◽  
Electrical Conductance ◽  
Ionic Concentration ◽  
Droplet Microfluidics ◽  
Cell Multiplication ◽  
Bacterial Cells ◽  
Label Free ◽  
The Road ◽  
Viability Assay

Bacteria cells use osmoregulatory proteins as emergency valves to respond to changes in the osmotic pressure of their external environment. The existence of these emergency valves has been known since the 1960s, but they have never been used as the basis of a viability assay to tell dead bacteria cells apart from live ones. In this paper, we show that osmoregulation provides a much faster, label-free assessment of cell viability compared with traditional approaches that rely on cell multiplication (growth) to reach a detectable threshold. The cells are confined in an evaporating droplet that serves as a dynamic microenvironment. Evaporation-induced increase in ionic concentration is reflected in a proportional increase of the droplet’s osmotic pressure, which in turn, stimulates the osmoregulatory response from the cells. By monitoring the time-varying electrical conductance of evaporating droplets, bacterial cells are identified within a few minutes compared with several hours in growth-based methods. To show the versatility of the proposed method, we show detection of WT and genetically modified nonhalotolerant cells (Salmonella typhimurium) and dead vs. live differentiation of nonhalotolerant (such as Escherichia coli DH5α) and halotolerant cells (such as Staphylococcus epidermidis). Unlike the growth-based techniques, the assay time of the proposed method is independent of cell concentration or the bacteria type. The proposed label-free approach paves the road toward realization of a new class of real time, array-formatted electrical sensors compatible with droplet microfluidics for laboratory on a chip applications.

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