scholarly journals UJI PREKLINIS VIRGIN COCONUT OIL TERHADAP PENINGKATAN KADAR KOLESTEROL BAIK HDL, PENURUNAN KADAR TRIGLISERIDA, PROFIL KIMIA ASAM LEMAK (C6-C18) DAN OMEGA-3 PADA SERUM DARAH TIKUS PUTIH (Mus musculus)

2015 ◽  
Vol 1 (1) ◽  
pp. 59
Author(s):  
Sumaryati Syukur ◽  
Rina Dahlyanti ◽  
Titi Sumanti ◽  
Yulia Murni ◽  
Zoni Hidayat ◽  
...  
Keyword(s):  
Coconut Oil ◽  
Capric Acid ◽  
Egg Yolk ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Omega 3 ◽  
Virgin Coconut Oil ◽  
Cholesterol Ratio ◽  
Group I

 ABSTRACT The Virgin Coconut Oil (VCO) products have different qualities and controversy effects of lipids metabolism. This research has been used one of VCO product that was produced by fermentation method using Lactobacillus sp. It has high amount of lauric acid (C12) up to 51 %, caprilic acid (C8) 8.9 %, capric acid (C10) 7 % include Omega-3, 6 and 9, vitamins A, D, E, K and three kinds of phytohormone. The ratio of Omega-3 and 6 was very proportional, therefore it is important to investigate the Pre Clinic Test of animal experiment. Pre Clinic Test of dietary VCO as food supplement has been determined by using 40 mice’s, which divided into 4 groups. Feeding on egg yolk to Group I (negative control), Group II (positive control) increased cholesterol level. The others were Group III (egg yolk and VCO 2 %) and group IV (egg yolk and VCO 4 %). It was determined the total of cholesterol, HDL at 10th, 20th and 30th day treated by using the enzymatic methods. The dietary of VCO 2-4 % resulted in significant increases in HDL levels from 32 % to 69 %. The dietary of VCO 4 % for four weeks did not toxic to mice metabolism. Triglycerides level decreased 50 % from 177 to 85 and similar resulted to cholesterol ratio. Feeding on VCO for 4 weeks, the SCFA and MCFA not detected in serum of mice. The LCFA (C16) palmitate in significant decreased from 0.96 to 0.1%. The significant level of Omega-3 increased more than three times in serum of mice dietary VCO 2-4 %.  Keywords: Coconut Oil, Lactobacillus

scholarly journals Immunomodulatory Effect of Virgin Coconut Oil in Wistar Rats Infected with Staphylococcus aureus

2021 ◽  
Vol 26 (1) ◽  
pp. 31
Author(s):  
Desy Cahya Widianingrum ◽  
Siti Isrina Oktavia Salasia
Keyword(s):  
Lymphocyte Proliferation ◽  
Histopathological Examination ◽  
Coconut Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Bacterial Cells ◽  
Immunomodulatory Effects ◽  
Virgin Coconut Oil ◽  
Sod Activity

<p class="abstrak1">Virgin coconut oil (VCO) contains bioactive that induce immunity against infectious diseases. This study aim to determine the immunomodulatory effects of VCO based on the activity of superoxide dismutase (SOD), lymphocyte proliferation, and histopathological examination in liver and kidney of rats infected with <em>Staphylococcus</em> <em>aureus</em>. The VCO was given intragastrically to rats with a dose of 250 µL for one week. The rats were infected with <em>S. aureus</em> at 5x10<sup>2</sup> bacterial cells intraperitoneally. Twenty (20) female <em>Wistar</em> rats of one month old were divided into four groups. The negative control group (C-): without treatment, AV group: infected with <em>S. aureus</em> followed by VCO treatment; VA group: pretreated with VCO followed by <em>S. aureus </em>infection, and positive control group (C+): were infected with <em>S. aureus</em> without VCO. All rats were euthanized and necropsied based on the animal ethic standard. Plasma samples were taken to evaluate SOD activity, and lymphocytes were isolated from the spleen to determine their proliferative ability. Livers and kidneys were collected for a histopathology examination. Results showed that the VA group had the highest SOD activity on the 4<sup>th</sup> week (41.50 ± 3.56 %) and lymphocyte proliferation (0.3018) compared to all treatments, indicating immunomodulatory effects of VCO. Liver of treatments group showed leucocytes infiltration, no hemorrhages (VA); the hepatocytes with normal cells (VA). Kidney of treatments group showed leucocytes infiltration (AV); normal epithelial glomerulus and tubulus cells, still found hemorrhage (VA). These studies indicated that VCO has a potential role as an immunomodulator, hepatoprotectant, and nephroprotectant.</p>

scholarly journals Nephroprotective effect of virgin coconut oil in Plasmodium berghei ANKA infected Balb/c mice

2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Syafarinah Nur Hidayah Akil ◽  
Heny Arwati ◽  
Puspa Wardhani ◽  
Priangga Adi Wiratama
Keyword(s):  
Plasmodium Berghei ◽  
Coconut Oil ◽  
Negative Control Group ◽  
Treated Group ◽  
Negative Control ◽  
World Health ◽  
Control Group ◽  
Plasmodium Berghei Anka ◽  
Virgin Coconut Oil ◽  
Nephroprotective Effect

AbstractMalaria is a parasitic infectious disease caused by Plasmodium, which remains a world health problem with an estimated 219 million cases worldwide. In severe malaria infection, several organs of the body can be affected, including the kidneys. One of the pathophysiology associated with the worsening of this disease is oxidative stress. The use of antioxidants is expected to prevent this, and one product that has a high antioxidant content is virgin coconut oil (VCO). This study aimed to analyze the effect of VCO on the kidney in Plasmodium berghei ANKA-infected mice. This study was an in vivo laboratory experimental study with a randomized post-test only control group design using 35 BALB/c mice infected with P. berghei ANKA, weighing 20-30 grams. VCO with the Javara® brand is used with doses of 1, 5, and 10 ml/kg body weight (kgBW)/ day. The parameter assessed were levels of BUN, creatinine, and renal histopathological changes. The administration of VCO on the treated group shows minimal tubular necrosis and glomerulonephritis compared to the negative control group. The BUN and creatinine levels in the treated group were also lower than the negative control group. The results showed that VCO has a nephroprotective effect against P. berghei ANKA infection in mice.Keywords: malaria, kidney, virgin coconut oil

scholarly journals The Combination Effect of Simvastatin and Virgin Coconut Oil on Total Cholesterol Levels in Dislipidemic Male Albino Rats (Rattus norvegicus)

2021 ◽  
Vol 12 (2) ◽  
pp. 72
Author(s):  
Lutfi Dewanda Nugroho ◽  
Soebagijo Adi Soelistijo ◽  
Jusak Nugraha
Keyword(s):  
Treatment Group ◽  
Total Cholesterol ◽  
Total Cholesterol Level ◽  
Coconut Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Cholesterol Levels ◽  
Group I

Introduction: Simvastatin is a primary pharmacological therapy for dyslipidemia (hypercholesterolemia). Virgin coconut oil (VCO) is a companion intake of statin drugs considered capable of lowering blood cholesterol levels. This study aimed to determine the combination effect of statin drug groups with VCO on total cholesterol.Methods: The stages of this study started with the adaptation process of animals, giving a high-fat diet (HFD), and treatment with simvastatin and VCO. The adaptation process was conducted where the experimental animals were fed pellets and drank clean water in a container. A HFD was given by mixing pork brains with distilled water with a ratio of 1:3 as much as 2 ml per day for 21 days. In simvastatin and VCO treatment for 7 days, the experimental animals were divided into 4 groups; group I was given aquades, group II was given VCO, group III was given simvastatin, and group IV was given simvastatin and VCO. The total cholesterol levels in the blood of albino rats were examined by employing a BS-300 Chemistry Analyzer through a spectrophotometric method. The total cholesterol levels data obtained were then examined for normality (Shapiro-Wilk test) and analyzed parametrically by utilizing One-way ANOVA.Results: The average total cholesterol level in the negative control group (being given a standard diet) was 45.57 mg/dL. The averages of blood total cholesterol levels were 47.28 mg/dL, 45.00 mg/dL, 46.85 mg/dL, 41.42 mg/dL, and 44.28 mg/dL in the positive control group (given an HFD), in the treatment group I, in the treatment group II, in the treatment group III, and in the treatment group IV, respectively. There were no significant distinctions in the statistical test on decreasing blood total cholesterol among the negative control group, positive controls I, II, III, and IV.Conclusion: Combination of simvastatin and VCO tends to reduce the total cholesterol level in albino rats. This is better than just giving VCO simvastatin alone.

scholarly journals ANTIBACTERIAL EFFECT OF VIRGIN COCONUT OIL ON (ACTINOMYCES SP.) THAT CAUSES DENTAL BLACK STAIN IN CHILDREN

2018 ◽  
Vol 11 (2) ◽  
pp. 333 ◽  
Author(s):  
Priscilla Lavine ◽  
Eva Fauziah ◽  
Mochamad Fahlevi Rizal ◽  
Sarworini Bagio Budiardjo
Keyword(s):  
Coconut Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Selective Medium ◽  
Staining Procedure ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Minimum Concentration ◽  
Significant Difference

 Objective: This study aimed to assess the effectiveness of virgin coconut oil (VCO) as a natural antibacterial agent to reduce the viability of the chromogenic bacteria Actinomyces sp., which causes dental black stains.Methods: Actinomyces sp. was isolated from the saliva of a child diagnosed with black stain. Each streak of bacteria was cultured on a selective medium Actinomyces agar and confirmed visually and through a gram staining procedure. Each bacterial culture was exposed to VCO in concentrations of 12.5%, 25%, 50%, and 100%. Afterward, viability testing with a methyl-thiazolyl-tetrazolium assay was conducted, and the results were read using an enzyme-linked immunosorbent assay reader.Results: The reduction of bacterial viability of Actinomyces sp. showed a significant difference between the negative control group and the groups treated with various concentrations of VCO 12.5%, 25%, 50%, and 100%.Conclusion: The minimum concentration of VCO necessary to kill Actinomyces sp. is VCO 12.5%.

scholarly journals EFFECTIVENESS OF OZONATED VIRGIN COCONUT OIL ON FULL THICKNESS SKIN DEFECT WOUND HEALING IN SPRAGUE DAWLEY Rats

2021 ◽  
Vol 8 (2) ◽  
pp. 220-228
Author(s):  
Herry Maha Putra Surbakti ◽  
Renny Yuniati ◽  
Djoko Handojo
Keyword(s):  
Coconut Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Full Thickness ◽  
Tgf Beta ◽  
Sprague Dawley ◽  
Virgin Coconut Oil ◽  
Sprague Dawley Rats ◽  
Significant Difference

AbstractIntroduction: Wounds are a breakdown of tissue integrity / continuity that can lead to infection. Virgin Coconut Oil is a processed coconut product made by processing fresh coconut flesh at low temperature and has a high nutritional content. Ozone therapy is an alternative therapy that has disinfectant properties and can induce strong oxidative stress. Methods: This study was conducted on 50 Sprague-Dawley rats that had injuries. We performed full thickness wounds and administered ozonated doses of VCO to mice. We looked at shrinkage of wounds and TGF Beta levels in mice. Assessment was carried out on day 7 and day 14 to assess the wound and TGF-beta immunohistochemically. Results: There was a significant difference in the wound shrinkage variable between the negative control group and the group that received VCO offerings both on day 7 and day 14. We also found a significant difference in the TGF-beta variable between the negative control group and the group that received VCO offerings both on day 7 and day 14. Conclusion: The administration of ozonated virgin coconut oil was effective in increasing the expression of TGF-? in the full thickness defect of Sprague Dawley rats and was effective in increasing the reduction in wound size in the full thickness defect of Sprague Dawley rats.

scholarly journals Cardioprotective effect of virgin coconut oil (VCO) on rats induced by doxorubicin

2019 ◽  
Vol 4 (3) ◽  
pp. 1-5
Author(s):  
Sukirman Lie ◽  
Nyoman Ehrich Lister I ◽  
Edy Fachrial ◽  
Jenny
Keyword(s):  
Free Radicals ◽  
Heart Muscle ◽  
Coconut Oil ◽  
Muscle Cells ◽  
Negative Control Group ◽  
Negative Control ◽  
Cardioprotective Effect ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Heart Muscle Cells

Cardiotoxicity is caused by anticancer drugs, one of the anticancer drug is doxorubicin. doxorubicin triggered free radicals reaction in heart muscle cells this results in the death of heart muscle cells or cardiomyopathy, in this condition, the heart can't pump blood properly and caused heart failure, virgin coconut oil (VCO) has antioxidant such as polyphenol that can neutralize the free radicals formed by doxorubicin. The aimed of this research was to evaluate the activity of cardioprotective effect of VCO on rats by induced doxorubicin with accumulative dose of 15 mg/kgbw for 21 days, by administering a 5 mg/kgbw dose in a week, and for 21 days of the rats given doses 2 VCO ml, 4 ml and 6 ml subsequently conducted an examination of CK-MB and LDH in the blood. The result showed that the effect cardioprotective from VCO reduce the levels of serum CK-MB (Creatinin kinase – MB) and the LDH (Lactate dehydrogenase) as the biomarker of heart. Group dose 6 ml showed the level of CK-MB 2110.37 ± 184,173 mg/dl and the level of LDH 2903.9 ± 70.0743 mg/dl and differ significantly (p < 0.05) with the negative control group induced doxorubicin. VCO is highly recommended to be food supplements for the patient used anti-cancer.

scholarly journals Efektivitas Ekstrak Artemisia vulgaris L. sebagai Hepatoprotektor pada sel-sel Hati Tikus yang Diinduksi Niasin

2018 ◽  
Vol 6 (2) ◽  
pp. 251
Author(s):  
Laily Rahmawati ◽  
Erma Sulistyaningsih ◽  
Rosita Dewi
Keyword(s):  
Treatment Group ◽  
Liver Damage ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Artemisia Vulgaris ◽  
Group I ◽  
Group Ii

  The niacin in energy drinks has metabolic product that cause oxidative stress and liver damage, while the liver damage can be prevented by hepatoprotective agents. Scoparone in Artemisia vulgaris L. can act as a hepatoprotector by its antioxidant effect. This study aimed to investigate the effectivity of Artemisia vulgaris L. extract as a hepatoprotector in wistar hepatocytes induced by niacin. This study used 25 male rats which were divided into 5 groups: normal, the negative control, the positive control, the treatment group I, and II. Treatment was conducted for 28 days. The samples were terminated and the hepatocyte were prepared for histological examination. Histological appearance was catagorized as mild, moderate, and severe damage with or without inflamatory cells activity. The data analysis by Kruskal Wallis showed significant difference (p<0,001). Further analysis by Mann Whitney revealed significantly difference (p<0,05) between normal group and all groups, negative control group and positive control group, and positive control group and treatment group I, but not significantly difference between negative control group and treatment group I, negative control group and treatment group II, positive control group and treatment group II, and between treatment groups. The study concluded that the effectivity of Artemisia vulgaris L. extract has not been proven as a hepatoprotector but further study is needed to draw a definite conclusion.   Keywords: energy drink, niacin, Artemisia vulgaris L., hepatoprotector  

scholarly journals Anti-nociceptive Potential of Ethanol Extract of Terminalia macroptera Guill&Perr (Combretaceae) Stem Bark in Mice

2021 ◽  
Vol 16 (2) ◽  
pp. 13-21
Author(s):  
S.A. Atunwa ◽  
M.O. Amali ◽  
S.O. Lawal ◽  
S.O. Usman ◽  
A.I. Olapade
Keyword(s):  
Acetic Acid ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Stem Bark ◽  
Negative Control ◽  
Flowering Plant ◽  
Control Group ◽  
Group I ◽  
Tail Immersion ◽  
Dose Dependent

Background: Terminalia macroptera Guill. &Perr. (Combretaceae) is a flowering plant with several ethno-medicinal claims. However, the dearth of information on its analgesic property has necessitated this study.Objectives: to evaluate the anti-nociceptive potential of ethanol extract of Terminalia macroptera stem bark (TMSB) in mice.Materials and Methods: Male and female mice of weight range 22 – 25g were randomly allotted into seven groups (n= 5) and treated as follows: Group I received 0.5 mL distilled water orally (negative control), Groups II-V were orally administered ethanol extract of T. macroptera stem bark (TMSB) at 50, 100, 200, and 400 mg/kg respectively while groups VI-VII received piroxicam 10 mg/kg and pentazocine 2 mg/kg intraperitoneally respectively as standards. The same treatment pattern was adopted for both pain models: tail immersion and acetic acid-induced writhing assays. Data were expressed as mean ± standard error of mean (SEM) using two-way analysis of variance (ANOVA) followed by Tukey’s and Bonferroni's multiple comparisons tests with p < 0.05 taken as significance.Results: The ethanolic extract of Terminalia macroptera stem bark showed significant dose-dependent anti-nociceptive activity at 100 and 400 mg/kg (2.95±0.41 and 2.9±0.31 respectively) 60 min post-treatment compared to the negative control group in the tail immersion test. Significant inhibition of nociception (0.20±0.20) was obtained at 400 mg/kg compared to the negative control group in the acetic acid-induced writhing test.Conclusions: The ethanol extract of Terminalia macroptera stem bark exhibited dose-dependent anti-nociceptive potential in both tail immersion and acetic acid-induced writhing assays in mice.

scholarly journals THE PROTECTIVE ROLE OF OMEGA-3 AGAINST GENOTOXICITY AND REPRODUCTIVE TOXICITY OF COBALT OXIDE NANOPARTICLES ACUTE TREATMENT IN MALE MICE

2018 ◽  
Vol 11 (5) ◽  
pp. 423
Author(s):  
Nahed A Hussien ◽  
Hanan R. H. Mohamed
Keyword(s):  
Cobalt Oxide ◽  
Negative Control Group ◽  
Protective Role ◽  
Negative Control ◽  
Control Group ◽  
Omega 3 ◽  
Genotoxic Potential ◽  
Polychromatic Erythrocytes

Objective: Cobalt nanoparticles (NPs), especially cobalt oxide NPs (Co3O4 NPs) are attracting unique shaped NPs that are used in different biomedical applications and medicine. Different in vitro studies report their toxic and carcinogenic effect but limited in vivo studies were present on its genotoxic potential. The present study was aimed to evaluate the genotoxic potential of Co3O4 NPs on bone marrow cells and sperms and the protective role of omega-3 in male albino mice.Methods: Animals were segregated into four groups that were orally treated for 3 consecutive days, Group 1: Negative control; Group 2: Omega-3 (250 mg/kg); Group 3: Co3O4 NPs (20 mg/kg); and Group 4: Combined group (250 mg/kg Omega-3 and Co3O4 NPs 20 mg/kg).Results: The present results show that Co3O4 NPs administration significantly increased number of micronucleated polychromatic erythrocytes (PCEs)/1000 PCEs, sperm abnormalities, and DNA damage, significantly decreased sperm motility and concentration in comparison to negative control group. However, Omega-3 administration in the combined group modulates the genotoxic potential of Co3O4 NPs in comparison to Co3O4 NPs group.Conclusion: The present study reports the genotoxic potential of Co3O4 NPs in vivo and assesses the protective role of Omega-3 administration due to its antioxidant effect.

scholarly journals Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study

2020 ◽  
Vol 8 (02) ◽  
pp. 55-59
Author(s):  
Reetu Arora ◽  
Yogesh Kumar ◽  
Neetu Jindal ◽  
Renu Aggarwal ◽  
Kavneet Takhar
Keyword(s):  
Root Canal ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I

Abstract Introduction The aim of obturation in the root canal is to completely seal the canal space to eliminate all the portals of entry and exit between root canal and periodontal space. Various techniques have been developed to achieve a hermetic seal. Materials and Methods As many as 150 extracted human maxillary central incisors were taken for the study. Biomechanical preparation was done up to F5 protaper file. According to different obturation techniques, samples were divided into six groups, keeping 30 samples in experimental and 15 samples in control groups. Group I–Lateral Condensation, Group II–Thermafil, Group III–Beefill, Group IV–GuttaFlow, Group V–Positive Control group, Group VI–Negative Control group. After obturation, the samples were immersed in 2% Rhodamine-B dye for 24 hours. Each sample was longitudinally sectioned to examine under confocal laser scanning microscope. Statistical Analysis The results were evaluated with ANOVA and posthoc Tukey honest significant difference (HSD) comparison test. Results The mean values of dye penetration of different groups were Group I (Lateral Condensation) 1.51 ± 0.451, Group II (Thermafil) 0.918 ± 0.399, Group III (Beefill) 1.30 ± 0.559. Group IV (GuttaFlow) 0.655 ± 0.396, Group V (Positive Control group) 1.96 ±0.046, Group VI (Negative Control group) 0 ± 0. The lowest mean value of apical microleakage was found in GuttaFlow amongst all experimental groups. Conclusion It can be concluded that the GuttaFlow obturating material exhibited better apical sealing ability with canal walls.

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