Turmeric and cinnamon dominate in antioxidant potential among four major spices

2017 ◽  
Vol 26 (1) ◽  
pp. 27 ◽  
Author(s):  
B Saranya ◽  
T Sulfikarali ◽  
S Chindhu ◽  
A M Muneeb ◽  
N K Leela ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Reducing Power ◽  
Black Pepper ◽  
Chloroform Extract ◽  
Antioxidant Potential ◽  
Total Phenol Content ◽  
Power Method ◽  
Phenol Content ◽  
Dpph Assay ◽  
Synthetic Antioxidant

Antioxidant activity of sequential extracts of black pepper, ginger, turmeric and cinnamon was determined by DPPH assay, phosphomolybdate method and ferric reducing power method and compared with that of the synthetic antioxidant BHA. The results revealed that methanol extract of cinnamon has highest antioxidant potential followed by chloroform extract of turmeric. The antioxidant potential was also correlated with total phenol content.  

scholarly journals Phytochemical Screening, Antimicrobial and Antioxidant Activities of Sonchus Oleraceus l (Leaves) Extracts

2020 ◽  
pp. 1-4
Author(s):  
Hatim MY Hamadnalla ◽  
◽  
Mahdi Abd Elmageed Mohammed ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Salmonella Typhi ◽  
Chloroform Extract ◽  
Antioxidant Potential ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Sonchus Oleraceus

The objective of this study to examine phytochemical analysis of sonchus oleraceus l, (asteraceae) (moliata) extracts which were extracted by four solvents, petroleum ether, chloroform, methanol and distill water and to assess their potential antibacterial against four standard bacteria gram-positive and gramnegative (bacillus subtilis staphylococcus aureu, salmonella typhi, and pscudomona aeruginosa) and antioxidant activities. (moliata) extracts using cold maceration method to extract sonchus oleraceus l, (asteraceae) locally known as (moliata) extracts, where dpph assay and paper disc diffusion assay were employed to evaluate the antioxidant and antibacterial activities respectively. The results showed that tannins, triterpenes, a sterols, flavonoids and cardiac glycosides compounds were present in the sonchus oleraceus l, (asteraceae) lives (moliata) extracts. The methanol extract of leaves showed high antioxidant potential (77± 0.03 % ) and chloroform showed low antioxidant potential activity (4±0.09) compare with propyl gallate as standard (93± 0.010 %) against dpph. Chloroform extract was effective and give moderate zone of inhibition against staphylococcus aureus. Methanol extract was effective and showed moderate zone of inhibition against staphylococcus aureus, bacillus subtilis, salmonella typhi and pseudomonas aeruginosa.

scholarly journals Phytochemical evaluation and in vitro antioxidant studies of selected species of Gomphostemma Wall. ex Benth. from Western Ghats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 32-37
Author(s):  
M. K Sajitha ◽  
J. E. Thoppil
Keyword(s):  
Hydroxyl Radical ◽  
Western Ghats ◽  
Quantitative Estimation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Aluminium Chloride ◽  
Dpph Assay ◽  
Radical Scavenging Assay

Plants are a rich source of bioactive compounds and have played a vital role in traditional medicine. Plant derived compounds are proved to have less side effects as compared to synthetic compounds. However, a truncated history on the contribution of plants to medicine together with lack of validation of plant derived components have led to massive research in phytochemistry and allied fields. Present study was intended to identify as well as to assess and compare different phytochemicals and antioxidant potential of selected taxa of Gomphostemma endemic to Western Ghats. Methanolic extracts of aerial parts of Gomphostemma heyneanum var. heyneanum, Gomphostemma heyneanum var. rottleri and Gomphostemma eriocarpum were subjected to preliminary phytochemical screening. Quantitative estimation of phenols and flavonoids was done with Folin-Ciocalteu method and modified Aluminium chloride method respectively. Antioxidant potential was examined by DPPH assay, Hydroxyl radical scavenging assay and Reducing power activity. Preliminary screening indicated the presence of many secondary metabolites like phenols, flavonoids, alkaloids, terpenoids and steroids. Results of quantitative estimation revealed that G. heyneanum var. heyneanum has the highest concentration of phenols and flavonoids. Antioxidant potential determined in terms of inhibition percentage also displayed that Gomphostemma species have significant radical scavenging activity. The antioxidant potential exhibited by the plant species may be attributed to their phenol and flavonoid content. Keywords: Phytochemical constituents, Gomphostemma, Quantitative estimation, Total phenolics, Flavonoids, Antioxidant activity, DPPH assay, Hydroxyl radical scavenging assay, Reducing power activity.

scholarly journals New Antioxidant Ingredients from Brewery By-Products for Cosmetic Formulations

Cosmetics ◽  
2021 ◽  
Vol 8 (4) ◽  
pp. 96
Author(s):  
Roberta Censi ◽  
Dolores Vargas Peregrina ◽  
Maria Rosa Gigliobianco ◽  
Giulio Lupidi ◽  
Cristina Angeloni ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Total Phenol ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenol Content ◽  
Hacat Cells ◽  
Phenol Content ◽  
Waste Products ◽  
Specific Product ◽  
Cosmetic Formulations

The purpose of this work was to evaluate the total phenol content and antioxidant activity of different types of handcrafted beers (Ego, Alter, Fiat Lux, Triplo Malto, Ubi, and Maior), as well as the starting materials (malts, hops, and yeast), the intermediate products, and the waste products (spent malts, hops, and yeast), in view of their use in innovative cosmetic formulations. Extractions from starting and spent samples were taken from water or 70° alcohol. The total phenol content (Folin Ciocalteau Essay) of all the brewing products depended on the specific product under investigation. The highest values were found in starting hops (ranging from approximately 93 to 155 mg GAE/g, according to the extraction solvent), intermediate ones in starting malt and starting yeast, and the lowest values in wort. The total phenol content in the final beers originates from the phenols that were extracted from the different ingredients, namely the starting malts, hops and yeast, but non-negligible values were still observed in spent products. The method used for the evaluation of the antioxidant activity, trolox equivalent antioxidant capacity (DPPH), ferric-ion reducing antioxidant parameter (FRAP), and radical cation scavenging activity and reducing power (ABTS) strongly influenced the results. In general, the results reflected the trend observed for the total phenol content: that beers are progressively enriched by phenols originating from all the starting ingredients, and that spent products still possess non-negligible antioxidant activity. It is interesting to note that waste yeast frequently showed higher values than those of the starting material; it can be inferred that yeast is able to absorb phenols from the beer during brewing. By considering the interest in exploiting waste derived from processing foods, the biological activity of waste Alter brewery products has been evaluated on a cell culture of keratinocytes (spent products of malt, hop, and yeast). Preliminary in vitro assays in keratinocyte HaCaT cells were carried out to assess the potential bioactivity of spent extracts. Among the spent extracts, the spent hop and yeast extracts showed the ability to improve the mitochondrial activity and prevent oxidative stress in HaCaT cells, two features in skin ageing. In conclusion, this study offers evidence that waste from handcrafted beers can be an interesting source of phenols for the preparation of skin anti-aging cosmetics.

scholarly journals Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Joham Sarfraz Ali ◽  
Hammad Saleem ◽  
Abdul Mannan ◽  
Gokhan Zengin ◽  
Mohamad Fawzi Mahomoodally ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Methanol Extract ◽  
Biological Activities ◽  
Chloroform Extract ◽  
Inhibitory Response ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Ms Analysis ◽  
Monotheca Buxifolia

Abstract Background Ethnobotanical and plant-based products allow for the isolation of active constituents against a number of maladies. Monotheca buxifolia is used by local communities due to its digestive and laxative properties, as well as its ability to cure liver, kidney, and urinary diseases. There is a need to explore the biological activities and chemical constituents of this medicinal plant. Methods In this work, the biochemical potential of M. buxifolia (Falc.) A. DC was explored and linked with its biological activities. Methanol and chloroform extracts from leaves and stems were investigated for total phenolic and flavonoid contents. Ultrahigh-performance liquid chromatography coupled with mass spectrometry (UHPLC–MS) was used to determine secondary-metabolite composition, while high-performance liquid chromatography coupled with photodiode array detection (HPLC–PDA) was used for polyphenolic quantification. In addition, we carried out in vitro assays to determine antioxidant potential and the enzyme-inhibitory response of M. buxifolia extracts. Results Phenolics (91 mg gallic-acid equivalent (GAE)/g) and flavonoids (48.86 mg quercetin equivalent (QE)/g) exhibited their highest concentration in the methanol extract of stems and the chloroform extract of leaves, respectively. UHPLC–MS analysis identified a number of important phytochemicals, belonging to the flavonoid, phenolic, alkaloid, and terpenoid classes of secondary metabolites. The methanol extract of leaves contained a diosgenin derivative and polygalacin D, while kaempferol and robinin were most abundant in the chloroform extract. The methanol extract of stems contained a greater peak area for diosgenin and kaempferol, whereas this was true for lucidumol A and 3-O-cis-coumaroyl maslinic acid in the chloroform extract. Rutin, epicatechin, and catechin were the main phenolics identified by HPLC–PDA analysis. The methanol extract of stems exhibited significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging activities (145.18 and 279.04 mmol Trolox equivalent (TE)/g, respectively). The maximum cupric reducing antioxidant capacity (CUPRAC) (361.4 mg TE/g), ferric-reducing antioxidant power (FRAP) (247.19 mg TE/g), and total antioxidant potential (2.75 mmol TE/g) were depicted by the methanol extract of stems. The methanol extract of leaves exhibited stronger inhibition against acetylcholinesterase (AChE) and glucosidase, while the chloroform extract of stems was most active against butyrylcholinesterase (BChE) (4.27 mg galantamine equivalent (GALAE)/g). Similarly, the highest tyrosinase (140 mg kojic-acid equivalent (KAE)/g) and amylase (0.67 mmol acarbose equivalent (ACAE)/g) inhibition was observed for the methanol extract of stems. Conclusions UHPLC–MS analysis and HPLC–PDA quantification identified a number of bioactive secondary metabolites of M. buxifolia, which may be responsible for its antioxidant potential and enzyme-inhibitory response. M. buxifolia can be further explored for the isolation of its active components to be used as a drug.

Effect of Grape (Vitis vinifera) Seed Extract on the Physico-Chemical, Microbial and Sensory Characteristics of Chevon Nuggets

2020 ◽  
Author(s):  
Priyanka Meena ◽  
Anurag Pandey ◽  
Ashish Saini ◽  
Asman Singh Gurjar ◽  
Ravi Raman ◽  
...  
Keyword(s):  
Frozen Storage ◽  
Seed Extract ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Antioxidant Ability ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Physico Chemical

The present study was carried out to evaluate the antioxidant potential of grape seed extract (GSE) and its effect on the physico-chemical, microbiological and sensory properties of chevon nuggets during frozen storage. Antioxidant potential by various biochemical tests (Antioxidant ability assays, total phenol content, total flavonoid content) were evaluated. The result showed that GSE exhibit high antioxidant ability assays (312.06±1.69 ìg ascorbic acid), total phenol content (190.34±2.26 mg GAE/g), total flavonoid content (83.43±2.36 mg rutin/g). The chevon nugget treated with GSE had significantly (P less than 0.05) lower thiobarbituric acid reactive substance (TBARS) and free fatty acid % (FFA) compared to control during frozen storage. Addition of GSE significantly (P less than 0.05) reduced the total plate, total psychrophilic and yeast and mold count in chevon nuggets during storage at -18±2oC. The GSE treated chevon nuggets recorded significantly (P less than 0.05) superior score of flavor, juiciness and overall acceptability than control. It can be concluded that GSE has excellent antioxidant properties, could be used as an antioxidant to meat products without affecting its quality and acceptability.

scholarly journals Dr. PI POLYPHENOL CONTENT, PHYTOCHEMISTRY COMPOSITIONS AND ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS FROM MARINE SPONGE AAPTOS SUBERITOIDES GROWN IN NHATRANG BAY, VIETNAM

2019 ◽  
pp. 80-86
Author(s):  
DANG XUAN CUONG ◽  
VU NGOC BOI ◽  
TRAN KHAC TRI NHAN ◽  
DINH HUU DONG ◽  
THAI MINH QUANG ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Marine Sponge ◽  
Functional Food ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Chloroform Extract ◽  
Polyphenol Content ◽  
Significant Difference ◽  
Dpph Scavenging

Objective: To investigate the content and antioxidant activities of polyphenol, the correlation between polyphenol content and their antioxidant activities, and phytochemistry compositions of different extracts from marine sponge Aaptos suberitoides commonly found growing in Nhatrang bay, Vietnam orienting application into functional food and pharmacy. Methods: Evaluating the toxicity of antioxidant polyphenol powder preparing from the initial concentrated extract was by the adjusted Behrens Karber method and a correlation between polyphenol content and antioxidant activities basing on the Pearson coefficient in Excel. Separating antioxidant polyphenol content was base on solvents polarization of n-hexane, chloroform, ethanol, ethyl acetate and n-butanol which the quantification of polyphenol content and antioxidant activities, and preliminary phytochemical compositions qualitative. Results: Antioxidant polyphenol powder did not affect mice weight during the assay time of 28 d. Polyphenol content and antioxidant activities got the highest value at chloroform extract in comparison to other extracts, a significant difference (p<0.05) and strong correlation (R2>0.9). Polyphenol content (122.682 mg gallic acid equivalent ml-1), total antioxidant activity (368.183 mg ascorbic acid equivalent ml-1), reducing power activity (24.08 mg FeSO4 equivalent ml-1) and DPPH scavenging (72.48±1.54 %) were the highest values. Alkaloids, flavonoids, steroids, tannins and triterpenoids existed in initial methanol extract. Weakly polarized polyphenol content was 70.27% in comparison to initial methanol extract. Conclusion: Antioxidant polyphenol of sponge Aaptos suberitoides has the potential for application into the field of functional food and pharmaceuticals.

scholarly journals Effect of Hippophae rhamnoides L. Leaves Treatment on the Antioxidant Capacity, Total Phenol Content and Sensory Profile of Moschofilero Wines Vinified with and without Added Sulphites

2020 ◽  
Vol 10 (10) ◽  
pp. 3444
Author(s):  
Alexandros Tzachristas ◽  
Konstantina Pasvanka ◽  
Maria Liouni ◽  
Antony C. Calokerinos ◽  
Panagiotis Tataridis ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenol ◽  
Sensory Profile ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Phenol Content ◽  
Color Intensity

This study evaluated the antioxidant capacity, total phenol content, and sensory profile of selected Greek wines made from Vitis vinifera L. c.v. Moschofilero in two consecutive vintages, treated with powdered Hippophae rhamnoides L. leaves (HRL). Radical Scavenging activity, reducing power, total phenol content (TPC), and color intensity increased in a linear manner in relation to HRL treatments. Indicatively the addition of 0.8 g/L of HRL increased the radical scavenging activity as determined via the inhibition of the 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical from 28.4 to 55.8% in comparison to the initial values. Equally the reducing power as determined by the ferric reducing antioxidant power (FRAP) assay increased from 35.3 to 62.1%, and total phenol content values increased from 11% to 23.7% and the color intensity increased from 39.9 to 50.7%. The main oenological attributes examined, remained unchanged after the HRL addition. The addition of up to 0.4 g/L of HRL did not have a major impact on the organoleptic characteristics of the wines tasted whereas concentrations higher than 0.8 g/L were not considered beneficial. Results denote that the addition of H. rhamnoides leaves to white wines contributes positively to the overall antioxidant capacity and could be used if authorized as an antioxidant agent in wines vinified in the absence of or in synergy with sulphur dioxide.

scholarly journals PHYTOCHEMICAL COMPOUNDS AND ANTIOXIDANT ACTIVITY OF YELLOW VELVETLEAF FRUIT (LIMNOCHARIS FLAVA) EXTRACT

2019 ◽  
pp. 55-57
Author(s):  
ACE BAEHAKI ◽  
SHANTI DWITA LESTARI ◽  
NORYATI SIREGAR
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Power Method ◽  
Activity Assessment ◽  
Sample Extraction ◽  
Phytochemical Compounds

Objective: The purpose of this research was to observe the content of phytochemical compound and antioxidant activity of yellow velvetleaf fruit (Limnocharis flava) extract. Methods: Research consisted of several stages, including sampling, sample preparation, sample extraction, calculation of yield extract, phytochemical analysis (flavonoids, steroids, alkaloids, saponins, and triterpenoids), and antioxidant activity assessment using 2’,2’-diphenyl-1-picrylhydrazyl and iron-reducing power method. Results: The n-hexane and methanol extract contained flavonoids, saponins, and triterpenoids, while flavonoids and triterpenoids were detected on ethyl acetate extract. The IC50 of yellow velvetleaf extract with n-hexane, ethyl acetate, and methanol was 3321.67 ppm, 1439.24 ppm, and 96.0 ppm, respectively. The methanol extract had the highest iron-reducing power with the average absorbance of 0.588 followed by the ethyl acetate extract with the average absorbance of 0.195 and n-hexane extract had lowest with the average absorbance of 0.171. Conclusion: The best solvent to extract the yellow velvetleaf and have highest antioxidant activity was methanol solvent.

scholarly journals Antioxidant Activity of Multifarious Solvent Extracts of Leaf. (Plumbago Zeylanica)

2019 ◽  
Vol 35 (2) ◽  
pp. 718-722
Author(s):  
D. Roselin Jenifer ◽  
B. R. Malathy
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Chloroform Extract ◽  
Assay Method ◽  
Dpph Assay ◽  
Leaf Extracts ◽  
Plumbago Zeylanica ◽  
Reducing Power Assay

The current investigation is focused to determine antioxidant activities of the leaf extract of Plumbago zeylanica. The leaf extracts were prepared by using multiform solvents with increasing polarity, including as hexane, chloroform, ethyl acetate and Methanol. The antioxidant activities enlisting DPPH assay, hydrogen peroxide assay, reducing power assay and nitric oxide assay method were performed for all extracts. Barring no one, the extracts had shown average to potent antioxidant activity. Uniquely, the ethyl acetate extract and the chloroform extract explicated the utmost antioxidant activity.

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