scholarly journals The inhibitory activity of plant extracts (Mentha Citrato L. and Citrus aurantium L.) towards some bacteria strains isolated from inflamed gums

2019 ◽  
Vol 24 (7) ◽  
pp. 40
Author(s):  
Nagham Nseif Jassim ◽  
Rashid Hamid Hassan
Keyword(s):  
Inhibitory Activity ◽  
Total Concentration ◽  
Bacterial Species ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Citrus Aurantium ◽  
Bacterial Isolates ◽  
High Inhibitory Effect

This study was conducted in Salahaldin for the period from 15/9/2018 to 15/3/2019, to study the inhibitory activity of aqueous and alcoholic (Eth,Meth) extract of  Mentha Citrato L. And Citrus aurantium L plants by agar well diffusion method on some bacterial causing gingivitis . including Staphylococcus aureus, Streptococcus faecalis and Klebsiella pneumonia, were isolated. The results showed that alcohol extracts had a high inhibitory effect on the growth of the bacterial species under study compared to the aqueous extracts. These bacterial isolates were primarily sensitive to the methanolic and ethanolic extract, followed by the hot aqueous extract . The study showed that Mentha Citrato L extract was more effective in inhibiting the growth of bacterial isolates compared to the extracts of the Citrus aurantium L. The inhibitory efficacy of these extracts increased with the concentration of the extract. The concentration was 100 (mg / ml) had a higher inhibitory value than the total concentration Study 20 and 60 (mg / ml) .   http://dx.doi.org/10.25130/tjps.24.2019.127

scholarly journals Effect of Capsicum grossum L. and Allium cepal L. Juice on some bacterial species isolated from contamiinated vegetable salad

2007 ◽  
Vol 4 (1) ◽  
pp. 35-40
Author(s):  
Baghdad Science Journal
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Inhibitory Activity ◽  
Bacterial Species ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Marked Activity ◽  
Agar Well Diffusion Method

Twenty four bacterial isolates were identified from (10) places for wandering sellers in south Baghdad city (Bayaa garage). They were Staphylococcus aureus (9 isolates), Bacillus subtilis (6 isolates), Salmonella spp. (4 isolates) and Psudomonas aeruginosa (5 isolates). Agar well diffusion method was used to definition sensitivity of the fresh and dried juice of Capsicum grossum L. and Allium cepal L. at different concentrations. The fresh juice had no inhibitory activity against the bacterial isolates in contrast to the fresh juice , dried juice which show marked activity against all bacterial isolates at (30) mg/ml.

scholarly journals Effect of ethanolic extract of Clove (Eugenia caryophyllus) on growth of some types of pathogenic bacteria

2009 ◽  
Vol 3 (1) ◽  
pp. 72-78
Author(s):  
Huda Suhail Abid
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Clove Extract ◽  
High Inhibitory Effect

Effect of ethanolic extract of Clove (Eugenia caryophyllus) examined against (4) species of pathogenic bacteria ('Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium) using: diffusion method, determination the minimum inhibitory concentration MIC, and minimum bactericidal concentration MBC. The results showed that Clove extract appeared high inhibitory effect on the growth of' 'Staphylococcus aureus & Pseudomonas aeruginosa, this inhibition increased with increasing the concentration. The MIC values were, 2.0, 4.0 mg/ml respectively, on the other hand, the MBC value against 'Staphylococcus aureus was 4.0 mg/ml. while the MBC value against Pseudomonas aeruginosa dose not effective. Further Escherichia coli & Salmonella typhimurium showed resistance to the activity of Clove extract.

scholarly journals The Effect of Aqueous and Alcoholic Extracts of Punica granatum L. Pericarp on Hemolysin Production of several Bacterial species

2010 ◽  
Vol 7 (1) ◽  
pp. 309-316
Author(s):  
Baghdad Science Journal
Keyword(s):  
Inhibitory Concentration ◽  
Bacterial Species ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Inhibitory Effect ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Punica Granatum L

Four local hemolysin producer bacterial isolates were selected, tow of them gram negative bacteria (Escherichia coli ,Pseudomonas aeruginosa ) and the other two were gram positive bacteria (Staphylococcus aureus , Bacillus cereus ). Minimum inhibitory concentration of the aqueous and alcoholic extracts of Punica granatum L. pericarp were determined towards the four bacterial isolates ,results obtaind showed that MICs of the aqueous extract were 200 mg/ml for E .coli and P. aeruginosa isolates while were 5 mg/ml and 2 mg/ml for B. cereus, S. aureus , respectively The MICs for the ethanolic extract were 50 mg/ml , 20 mg/ml ,1 mg/ml ,0.5 mg/ml for E. coli ,P. aeruginosa ,B. cereus ,S. aureus , respectively. The effect of Sub-MICs of the aqueous and alcoholic extracts on hemolysin production was investigated , both extracts had a suppressing effect on hemolysin production by E. coli ,P. aeruginosa ,while both extract had an inhibitory effect on hemolysin production by S. aureus and B . cereus isolates

scholarly journals Control of Clinical Pathogens by the Haemolymph of Paratelphusa hydrodromous, a Freshwater Crab

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
A. Arul Prakash ◽  
S. Balasubramanian ◽  
G. Gunasekaran ◽  
M. Prakash ◽  
P. Senthil Raja
Keyword(s):  
Bacterial Species ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Disc Diffusion ◽  
Freshwater Crab ◽  
Disc Diffusion Method ◽  
Strong Response ◽  
Clinical Pathogens ◽  
Rhizopus Sp ◽  
Antimicrobial Assay

In the present study, effort has been made to find the antimicrobial activity of haemolymph collected from freshwater crab, Paratelphusa hydrodromous. The haemolymph collected was tested for antimicrobial assay by disc diffusion method against clinical pathogens. Five bacterial species, namely, Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus, and five fungal strains, namely and Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Rhizopus sp., and Mucor sp., were selected for the study. The result shows a strong response of haemolymph against the clinical pathogens which confirms the immune mechanism of the freshwater crab.

scholarly journals Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities

2021 ◽  
Vol 3 (8) ◽  
Author(s):  
Muhammad Yasir ◽  
Basit Zeshan ◽  
Nur Hardy A. Daud ◽  
Izzah Shahid ◽  
Hafza Khalid
Keyword(s):  
Antimicrobial Activity ◽  
Inhibitory Activity ◽  
Antifungal Drugs ◽  
Proteinase K ◽  
Diffusion Method ◽  
List Type ◽  
Bacterial Isolates ◽  
E Coli ◽  
Inhibitory Potential

Abstract There is a need for more efficient and eco-friendly approaches to overcome increasing microbial infections. Bacteriocins and chitinases from Bacillus spp. can be powerful alternatives to conventional antibiotics and antifungal drugs, respectively. The purpose of this study was to assess the inhibitory potential of bacteriocins and chitinase enzymes against multiple resistant bacterial and fungal pathogens. Bacterial isolates were selected by growth on minimal salts medium and after that were morphologically and biochemically characterized. The physiochemical characterization of bacteriocins was carried out. The inhibitory potential of bacteriocins towards six pathogenic bacteria was determined by the well diffusion assay while chitinase activity towards three fungal strains was determined by the dual plate culture assay. Two bacterial strains (WW2P1 and WRE4P2), out of nine showed inhibition of K. pneumonia, P. aeruginosa, E. coli and MRSA while WW4P2 was positive against S. typhimurium and E. coli and WRE10P2 against P. aeruginosa, S. pneumoniae. Two bacterial isolates (WW3P1 and WRE10P2) were chosen for further study on the basis of their antifungal activities. Of these, WW3P1 isolate was more effective against A. fumigatus as well as A. niger. The proteinaceous nature of the bacteriocins was confirmed by treatment of the crude extract with proteinase K. It was found that the inhibitory activity of strain WW3P1 against E. coli was highest at 20 °C, and against S. pneumoniae it was at 20 °C and pH 10 after treatment with EDTA. Inhibition by strain the WRE10P2 against P. aeruginosa was highest at 20 °C and pH 14. It was found that EDTA increased the inhibitory activity of strain WW2P1 against P. aeruginosa, K. pneumoniae and E. coli by 2 ± 0.235, 3.5 ± 0.288, 2.5 ± 1.040 times, respectively, of strain WRE4P2 against P. aeruginosa and E. coli by 2.5 ± 0.763, 2.7 ± 0.5 times, respectively, and of strain WRE10P2 against S. pneumoniae by 3 ± 0.6236 times. The isolates have promising inhibitory activity, which should be further analyzed for the commercial production of antimicrobials. Article highlights The current study aimed to isolate the microbiome from wheat plant (Triticum aestivum L.), to screen for bacteriocin production and to assess its antimicrobial activity against human pathogens. Forty-one phenotypically different bacterial colonies were subjected to bacteriocin purification from which 25 colonies showed positive reactions. These 25 bacterial isolates were screened against six different human bacterial pathogens using the well diffusion method to check the antimicrobial activity. Out of nine bacterial isolates, WW3P1 and WRE10P2 were able to degrade the chitin and utilize it as their sole energy source. Strain WRE4P2 exhibited partial inactivation in its activity against MRSA after treatment with proteinase K.

scholarly journals Development of mouthwash with Rosmarinus officinalis extract

2014 ◽  
Vol 50 (4) ◽  
pp. 851-858 ◽  
Author(s):  
Isabela Moreira Baumgratz de Paula ◽  
Flávia Costa Moraes ◽  
Orlando Vieira de Souza ◽  
Célia Hitomi Yamamoto
Keyword(s):  
Sodium Fluoride ◽  
Inhibitory Activity ◽  
Raw Materials ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Rosmarinus Officinalis ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Oral Infections

Rosmarinus officinalis, which belongs to the Lamiaceaefamily, is a species of medicinal flora with therapeutic properties. In order to exploit the benefits of these properties, a mouthwash formulation was developed, with careful selection of raw materials to meet pharmacotechnical requirements. Extracts of the plant were incorporated into a mouthwash, which was shown to have inhibitory action in vitro against the micro-organisms commonly found in periodontics. Controls for assessing the quality of the drugs were carried out, quantifying phenols and flavonoids as chemical markers. Mouthwash solutions were formulated containing 0.1, 5 and 10% ethanol extract of R. officinalis; and 0.05, 5 and 10% of the hexane fraction of R. officinalis. In order to evaluate synergism, ethanol extract and hexane fraction were also added to formulations containing 0.05% sodium fluoride and 0.12% chlorhexidine digluconate. These formulations were assessed for inhibitory effect against the specific microorganisms involved in the process of bacterial plaque formation, S. mutans(ATCC25175) and C. albicans(ATCC 10231), frequently found in cases of oral infections. The agar diffusion method was used to evaluate the inhibitory activity of extracts and formulations. All mouthwash solutions displayed inhibitory activity having higher sensitivity to S. mutansfor the 5% ethanol extract+0.05% sodium fluoride, and greater sensitivity to C. albicansfor the 10% hexane fraction. Results were characterized by the appearance of a growth inhibition halo, justifying the utilization and association of extracts of R. officinalis.

scholarly journals The Carpathian lingonberry, raspberry and blackberry fruit extracts feature variable antimicrobial efficiency

2019 ◽  
pp. 27-32
Author(s):  
Emőke Mihok ◽  
Éva György ◽  
Endre Máthé
Keyword(s):  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Effects ◽  
Fruit Extracts ◽  
Gram Positive Bacteria ◽  
Wild Berries

Wild berry is an excellent source of phytonutrients and/or bioactive compounds associated with significant therapeutic properties, so that they have been utilized in folk medicine and traditional nutrition throughout centuries. Multiple health-promoting effects, such as anti-inflammatory, anti-diabetic, anti-heart and coronary disease properties were attributed to such wild berries. It has also been proved that berries could feature antimicrobial effects that could be of a great importance for the prevention of food-feed poisoning and fighting back antibiotic resistance. In this study, we investigated the antimicrobial properties of lingonberry (Vaccinium vitis-idaea), raspberry (Rubus idaeus) and blackberry (Rubus fruticosus) crude and ethanolic extracts prepared from fruits obtained from the spontaneous flora of Eastern Carpathian Mountains situated in Transylvania. The antimicrobial effect of crude and alcoholic extracts were assessed on four Gram-negative, five Gram-positive bacteria and one yeast species using the agar diffusion method. The studied bacteria can cause food or feed spoilage and foodborne diseases. Our results indicate the significant inhibitory effect of lingonberry extracts in the case of Gram-negative bacteria like Proteus vulgaris and Salmonella Hartford, while among Gram-positive bacteria the strongest inhibitory effect was observed for Bacillus species like B. cereus, B. subtilis, B. mojavensis and Micrococcus luteus. The raspberry and blackberry extracts featured milder inhibitory effects in the case of the studied bacteria species. Furthermore, we have studied the crude or ethanolic extract combinations associated antimicrobial effects synergistic/additive or antagonistic properties. Interestingly, the triple and double ethanolic extract mixes had stronger antimicrobial properties, whereas the crude extract mixes showed relatively reduced effects, if any. Our results indicate that the antimicrobial activity of studied fruit extracts obtained from wild berries can vary upon the applied extraction method and their combination formulae, so that all these considerations must be taken into account when such fruit extracts are considered for foodstuff development.

scholarly journals Antibacterial Effect of Vernonia anthelmentica (Kattu Seeragam) on Bovine Mastitis

2021 ◽  
Vol 7 (2) ◽  
Author(s):  
Sandhiya G ◽  
Ahmed Fauzia
Keyword(s):  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Bacterial Species ◽  
Bovine Mastitis ◽  
Seed Extract ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Common Disease ◽  
Phytochemical Analysis ◽  
Quality Of Milk

Bovine mastitis is the common disease of mammary glands of dairy cows, caused by pathogenic bacteria, such as Staphylococcus aureus, Enterococccus faecium, Escherichia coli, Klebsiella pneumonia, Bacillus etc. The disease severely impacts the quality of milk and associated milk products. Three different bacterial species Staphylococcus, Bacillus and Klebsiella were isolated, identified and later treated with the seed extract of Vernonia anthelmentica. Well diffusion method was used to test the antibacterial effect of ethanol seed extract of Vernonia anthelmentica. Results showed that Vernonia anthelmentica was most effective against Staphylococcus compared to other two bacterial species. Phytochemical analysis of Vernonia anthelmentica revealed the presence of tannins, saponins, terpenoids etc. Present study suggests that seeds of Vernonia anthelmentica can be an effective antibacterial against bovine mastitis, which might be due to thepresence of tannins, and terpenoids in the extract.

scholarly journals Assessment of microbiological and residual antibiotics status in milk sold in Abeokuta, Ogun State. Nigeria

2018 ◽  
Vol 6 (6) ◽  
pp. 642
Author(s):  
Flora Oluwafemi ◽  
Bosede Folashade Oluwabamiwo ◽  
Oluseyi Oluwajubelo Oluwatosin ◽  
Olufunmilayo Ruth Akinrinade ◽  
Adelodun Lawrence Kolapo
Keyword(s):  
Bacterial Species ◽  
Method Comparison ◽  
Antibiotic Sensitivity ◽  
Plate Count ◽  
Diffusion Method ◽  
Rrna Gene ◽  
Antibiotic Residues ◽  
Bacterial Isolates ◽  
Powdered Milk ◽  
Microbiological Methods

This study investigated the prevalence and concentration of antibiotic residues in twenty brands of milk. Determination and quantification of antibiotic residues was done using High Performance Liquid Chromatography. Aerobic plate count was carried out and the associated bacteria isolated. Identification of bacteria was done using standard microbiological methods. Molecular characterization and identification of bacterial isolates was done using 16S rRNA gene sequencing method. Antibiotic sensitivity was carried out on bacterial isolates using disc diffusion method. Plasmid profile of drug resistant isolates was done using alkaline analysis method. Comparison of means was done using Analysis of Variance. Antibiotic residues were detected in 10 (50%) of the 20 milk brands analyzed. The residual levels of tetracycline, oxytetracycline and chlortetracycline ranged between 5ng/kg and 1569ng/kg while none of the samples had doxycycline residues. The prevalence of tetracycline residues in evaporated milk brands and powdered milk samples were 100 and 23 % respectively. The aerobic plate counts ranged from 2.5 × 102 to 6.5 × 102 CFU∕mL for evaporated milk and 2.5 × 101 to 6.0 × 101 CFU∕mL for powdered milk. A total of ten bacterial species were isolated and identified. Susceptibility result showed that 95% of the isolates were sensitive to erythromycin, cefuroxime and gentamycin; 12.5% were resistant to tetracycline and ampicillin, 10% were resistant to ofloxacin and 7.5% to doxycycline and penicillin. Five of the bacterial isolates were resistant to more than one class of antibiotics. Resistant isolates subjected to plasmid profiling had detectable plasmids with estimated sizes between 120bp and 1000bp. The obtained results provide evidence that the presence of antibiotic residues in evaporated and powdered milk is an indication that the public is exposed to the harmful effects of the residues.

scholarly journals Bacteriological Quality Assessment of Nigerian Naira Notes in Circulation at Aminu Kano Teaching Hospital, Kano State, Nigeria

2020 ◽  
Vol 5 (2) ◽  
pp. 148-153
Author(s):  
Zainab S. Na'inna
Keyword(s):  
Teaching Hospital ◽  
Bacterial Species ◽  
Hospital Environment ◽  
Health Concern ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Coagulase Negative Staphylococci ◽  
Bacteriological Quality ◽  
Microbiological Methods

Contamination of currency notes by pathogenic microorganism is a public health concern. An improved understanding of the bacteriological quality of currencies would help us better understand the role of money as a medium of transmitting infections. This study investigates the bacteriological quality of Nigerian currencies circulating in Aminu Kano Teaching Hospital (AKTH) in Kano metropolis. A total of 128 Naira note samples containing 16 pieces of each denomination of 5, 10, 20, 50, 100, 200, 500 and 1000 were aseptically collected from different cash units of the hospital and subjected to standard microbiological methods for the enumeration and isolation of bacteria. The susceptibility of the bacterial isolates to antibiotics was investigated using disk diffusion method. The mean aerobic mesophilic bacterial count of the currency notes range between 3.10 x104 cfu/mlto 5.25 x104 cfu/ml. Different bacterial species were isolated which include E. coli, Klebsiella spp, Staphylococcus aureus and Coagulase negative Staphylococci, with S. aureus having the highest frequency of occurrence of 37 (46.2 %). Findings of antibacterial susceptibility test indicated that100% of the isolated Gram negative bacteria were susceptible to Meropenem, followed by susceptibility to Gentamycin and Ciprofloxacin. However, the isolates were found to be highly resistant to Augmentin (55%) and Cefuroxime (32%). On other hand, the Gram positive bacterial isolates were highly susceptible to Erythromycin (84%), Gentamycin(83%), Clindamycin (79%),and Oxicillin (78%) while few of the isolate showed resistance to Penicillin(24%), Oxacillin (22%), and Clindamycin, (16%). The study reveals that currency notes in circulation at AKTH may serve as vehicles for the spread of disease causing organisms. Cashless transactions and use of hand sanitizers is therefore recommended to reduce the risk of infection. Key words: Naira notes, Contamination, Hospital environment

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