scholarly journals EFEKTIVITAS EKSTRAK KULIT BATANG MAJA (Aegle marmelos L) TERHADAP KEMATIAN LARVA NYAMUK Anopheles sp.

2021 ◽  
Vol 15 (2) ◽  
pp. 67
Author(s):  
Sinta Debi Pratama ◽  
Prayudhy Yushananta

<em>Malaria is one of the public health problems that can cause death in infants, children under five, pregnant women and reduces work productivity. Vector control by chemical means leads to vector resistance and environmental pollution and negatively impacts public and animal health. This study aims to determine the effectiveness of the bark extract of Maja (Aegle marmelos L) against the death of Anopheles sp mosquito larvae with concentrations of 5%, 10%, 20%, 40%, and 50% and the observation of contact times every 15 minutes, 30 minutes, 60 minutes, 90 minutes and 120 minutes. The study used a Completely Randomized Design (CRD) with Factorial. Extraction used maceration method, with 70% ethanol as solvent. Larvae of Anopheles sp. instar III obtained from malaria endemic area. The test used a 200 ml container, with two replications. All data were analyzed by two way ANOVA and Tukey HSD test. This study obtained mortality of 93.3% larvae at a concentration of 50% and a contact time of 120 minutes. The results of statistical analysis showed a significant effect of concentration (p=0.0001), contact time (p=0.0001), and interaction concentration-contact time (p=0.004) on the mortality of Anopheles sp. mosquito larvae. This study has proven the use of bark extract of Maja (Aegle marmelos L) as an alternative bio-insecticide in controlling mosquito larvae of Anopheles sp.</em>

2020 ◽  
Vol 2 (1) ◽  
pp. 72-78
Author(s):  
Thadeus Mariode Triputra Purba ◽  
Irwan Effendi ◽  
Feliatra Feliatra

This research was conducted in January - February 2019. The purpose of this study was to examine the effectiveness of mangrove extracts on stems and fruit on the growth of Aedes aegypti mosquito larvae and to know Lethal Concentration LC50 and Lethal Time LT50. The method used is the experimental method, with the experimental design is a completely randomized design (CRD). The treatment of nipah fruit and stem extract concentrations was 3%, 6% and 9% with contact time of 15 minutes, 30 minutes, 45 minutes 60 minutes, 120 minutes, 480 minutes and the number of replications 3 times with 2 trials. To find out (LC50) and (LT50), the data was analyzed using the SPSS program. The sample of this research is stem and nipah fruit taken in the mangrove area of Kayu Ara Village, Siak Regency. Aedes aegypti larvae are taken at a water reservoir next to the Arfaunas Mosque in Tampan District. The results showed that the stem and nipah fruit extracts had effective killing power against Aedes aegpti larvae. The extract that was more effective in killing mosquito larvae was the nipah fruit extract compared to the nipah stem extract seen in the number of larval deaths with LC50 = 3.398% and LT50 = 3.540 hours. This shows that the level of toxicity is quite toxic.


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Wahyuzan Wahyuzan ◽  
Lukmanul Hakim ◽  
Rahmat Afrizal ◽  
Asmeri Lamona ◽  
Khairuni Khairuni ◽  
...  

Traditional medicine in Indonesia has been known by the public before formal health services using modern medicine. Indonesia has many type of herbal plants which have medicinal properties including dadap (Erythrina orientalis). This study aims to utilized dadap herbal liniment by modifying heating in Vigin Cocanut Oil. The study conducted in the laboratory of Agricultural Product Processing using a Factorial Completely Randomized Design (CRD). The first factor is the addition of 250 ml (M1), 375 ml (m2), and 400 ml (M3) Virgin Cocanut Oil treatment. The second factor is the heating tim of 5 minutes (L1), 10 minutes (L2), and 15 minutes (L3). Observation variable consisted of yield, Specific gravity, and organoleptic test of aroma and colored. The result showed an average yield of 87.70-91.88%. The highest yield was found in the treatment of adding 400 ml Virgin Cocanut Oil and 15 minutes of heating time. The highest type of weight was found in the treatment of addition of 325 ml Virgin Cocanut Oil and 5 minutes heating time. Based on the organoleptic scent test, the everage penalist gives a like answer. Organoleptic colors of reudeuep herbal products is according to SNI number 06-23-2006.


KOVALEN ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 308-314
Author(s):  
Musafira Musafira ◽  
Nurfitrah M Adam ◽  
Dwi Juli Puspitasari

The investigation about the utilization of Banana peel (Musa paradisiaca) as biosorbent Rhodamine B dye has been done The purpose of this study was to determine the maximum contact time and to determine the adsorption capacity of kepok banana peel. Completely randomized design (CRD) was used in this research with two variables (the contact time and Rhodamine B concentration. Both variables were done in five levels i.e 10, 30, 60, 90, and 120 min and 2, 4, 6, 8 and 10 ppm respectively. The result showed that the maximum concentration of banana peel in adsorbing Rhodamine B was 6 ppm with 120 of contact time, and Rhodamine B adsorption capacity was  4.55mg/g. Keywords: Banana peel, Rhodamine B, biosorbent


Author(s):  
Anne Caroline Dallabrida Avelino ◽  
Dayana Aparecida De Faria ◽  
Carlos Eduardo Avelino Cabral ◽  
Joadil Gonçalves De Abreu ◽  
Elisangela Clarete Camili ◽  
...  

The aim of this study was to evaluate the effects of Brachiaria seed contact time with 05-25-15 fertilizer (N-P2O5-K2O) on the seed’s physiological quality. The experimental design was a completely randomized design with four replicates. Treatments were arranged in a 5x4 factorial, being: five seeds contact times with fertilizer (0, 24, 48, 72 and 96 hours); four forages: Marandu palisadegrass, Piatã palisadegrass, Xaraés palisadegrass and Ruziziensis grass. The performed tests were: water content, germination (first count and germination percentage), tetrazolium (viability), electrical conductivity, accelerated aging, sand emergency and emergence speed index. Contact time of 05-25-15 fertilizer with Brachiaria seeds reduces linearly: Germination, emergence and emergence speed index of Brachiaria seeds; Piatã and Xaraés palisadegrass seeds viability; vigor (electrical conductivity), except in Ruziziensis grass seeds; and seeds germination of Piatã palisadegrass submitted to accelerated aging test (vigor). Piatã palisadegrass is the least recommended, among the analyzed grasses, for intercropping in simultaneous sowing.


Author(s):  
A. J. Gomes Filho ◽  
S. C. de Paiva ◽  
G. M. C. Takaki ◽  
A. S. Messias

This study aims to analyze the reject produced by the desalinators implanted in the semi-arid Pernambuco in contact with the seeds of Moringa oleifera Lam. For this, Moringa seeds were collected and prepared with the following treatments: reject (control), reject with whole seed with and without husks, reject with crushed seed with and without husks, seed residue with and without husks, in five replicas. Each replica was constituted with doses equivalent to 2.0 g of Moringa seeds for 200 mL of reject, with contact time corresponding to 30, 60, 120 and 180 minutes, in a completely randomized design, under laboratory conditions, total of 140 experimental units. The physical-chemical and statistical analyses were performed through analysis of variance (ANOVA), and the F test, at confidence interval of 95%. It has been found through laboratory tests that seeds with crushed or ground Moringa husks are equally effective at adsorbing sodium from 1,868,0 mg/L to 24,6 mg/L (98,7 %), calcium from 1,005.0 mg/L to 894.6 mg/L (11%), magnesium from 741.0 mg/L to 728.3 mg/L (1.7%) and chloride from 6,997.5 mg/L to 6,782.4 mg/L (1.8%). Therefore, Moringa seed can be considered promising in the adsorption of chemical elements of saline water desalinizers.


2019 ◽  
Vol 11 (2) ◽  
pp. 87-93
Author(s):  
Wuri Artikasari ◽  
Emantis Rosa ◽  
Bambang Irawan

DHF (Dengue Hemorrhagic Fever) is a serious problem in Indonesia. DHF disease control has been applied so far, one of which is the use of larvacide temephos (abate). However larvacide is a chemical insecticide that has a negative impact on human health and causes resistance. Therefore in this research, biological control is carried out by utilizing entomopathogenic fungi as a larvacide. The purpose of this study was to determine the effectiveness of entomopathogenic fungi isolated from Ae aegypti larvae. Against the death of Ae. Aegypti mosquito larvae with the moist chamber method. This study used a completely randomized design (CRD) with a factorial pattern and performed two repetitions. Factor A is a type of fungi with 3 levels, namely A1: Aspergillus sp1, A2: Aspergillus sp2, and A3: Syncephalastrum sp. Factor B is a dilution with 7 levels, namely B0: Control, B1: 100 (without dilution), B2: 10-1, B3: 10-2, B4: 10-3, B5: 10-4, B6: 10-5 with every treatment was applied in 2 repetitions. Observations were made 24 hours after treatment for 3 days. Data were analyzed using analysis of variances (anova) and continued with the Least Significant Difference Test (LSD) at 5%. The results indicate that fungi isolates are the most effective in killing Ae. Aegypti mosquito larvae is Aspergillus sp1 and Aspergillus sp2 on the treatment of spores without dilution. Key words: DHF; larvacide; Aedes aegypti; Entomopathogenic Fungi.


Masker Medika ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 226-232
Author(s):  
Triana Oktaviani ◽  
Zairinayati Zairinayati

Latar Belakang: Aedes aegypti merupakan nyamuk yang dapat berperan sebagai vektor Demam Berdarah Dengue (DBD). Upaya promotif dan preventif menjadi mutlak dilakukan untuk memutus mata rantai penularan dengan membunuh larva nyamuk dengan larvasida alami menggunakan ekstrak daun sirih (Piper betle) sebagai insektisida alami. Tujuan dari penelitian: ini adalah untuk mengetahui perbedaan abate dan ekstrak daun sirih (Piper betle) dalam mematikan larva Aedes aegypti L instar III. Desain penelitian: ini bersifat eksperimen dengan menggunakan rancangan acak lengkap (RAL) yang terdiri dari 3 perlakuan 1 kontrol dan 8 kali pengulangan percobaan. Hasil Penelitian: dapat diketahui bahwa untuk penggunaan ekstrak daun sirih (Piper betle) dengan konsentrasi 5% dapat mematikan larva sebanyak 8 ekor, sedangkan konsentrasi 10% tidak dapat mematikan larva (0 larva) dan konsentrasi 15% dapat mematikan larva sebanyak 17 (85%) sedangkan untuk penggunaan abate dengan konsentrasi 5%, 10% dan 15% dapat mematikan larva sebanyak 160 larva (100%). Kesimpulan: bahwa abate mempunyai efektifitas lebih tinggi di banding dengan ekstrak daun sirh (Piper betle).     Background: Aedes aegypti is a mosquito that can act as a vector of dengue Hemorrhagic Fever (DHF). Promotove and preventive effort are absolutely necessary to break the chain to transmission by killing mosquito larvae with natural larvacides using betel leaf extract (Piper betle) as a natural insecticides. The purposes of this study: this was to determine the differences in abate and betel leaf extract (Piper batle) in kliing Aedes aegypti instar III larvae. Research methods this is an experimental study using a completely randomized design (CRD) consisting of 3 treatments 1 control and 8 repetitions of the experiment. The results: it can be seen that for the use of betel leaf extract(Piper betle) with a concentration cannot kill larvae in 17 (85%) whilke for the use of abate with a concentration of 5%, 10% and 15% can kill larvae of 160 (100%). Conclusion: that the abate has a higher effectiveness compared to sirih leaf extract.


2018 ◽  
Vol 12 (1) ◽  
pp. 33-38
Author(s):  
Arif Nur Muhammad Ansori ◽  
Hebert Adrianto ◽  
Hamidah Hamidah

Abstract The development of resistance to chemical insecticides against mosquitoes has beenconsidered as a setback in vector control. This study was aimed to identify LethalConcentration 90% (LC90) of polar fraction extract from  Citrus hystrix and Citrusaurantifolia leaves as biolarvicide against mosquito larvae after 24 hours of exposure.This study was an experimental research based on a Completely Randomized Design(CRD). The polar fraction extract of C. hystrix and C. aurantifolia  were tested in concentrations of 0 ppm, 500 ppm, 1375 ppm, 2250 ppm, 3125 ppm, and 4000 ppm againstthe 3rd instar larvae of  Culex quinquefasciatus. Each treatment was done in five independent replications. The numbers of deceased mosquito larvae were determinedafter 24 hours of treatment and analyzed by probit. The results showed that the polarfraction extract of  C. hystrix and C. aurantifolia  have bio-larvicidal activity against the 3rdinstar larvae of Cx. quinquefasciatus at LC90 of 1,653 ppm and 2,797 ppm, respectively. In conclusion, the polar fraction extract of C. hystrix leaf is more toxic than C. aurantifolialeaf's against  Cx. quinquefasciatus larvae. Abstrak Perkembangan resistensi terhadap insektisida kimia di antara spesies nyamuk telahdianggap sebagai kemunduran dalam pengendalian vektor. Penelitian ini bertujuanuntuk mengetahui Lethal Concentration 90% (LC90 ) ekstrak fraksi polar daun  C. hystrix dan C. aurantifolia sebagai biolarvasida terhadap larva nyamuk setelah paparan 24 jam. Penelitian ini adalah penelitian eksperimental dengan Rancangan Acak Lengkap (RAL).Ekstrak fraksi polar daun C. hystrix dan C. aurantifolia  diuji dengan konsentrasi 0 ppm,500 ppm, 1.375 ppm, 2.250 ppm, 3.125 ppm, dan 4.000 ppm terhadap larva nyamuk Cx.quinquefasciatus instar III. Setiap konsentrasi dilakukan replikasi sebanyak lima kali.Jumlah larva nyamuk yang mati dihitung setelah 24 jam. Setelah itu, data dari larvanyamuk yang mati dianalisis menggunakan probit. Hasil penelitian ini mengungkapkanbahwa ekstrak fraksi polar daun C. hystrix dan C. aurantifolia memiliki aktivitas sebagai biolarvasida terhadap larva nyamuk Cx. quinquefasciatus instar III dengan LC 90= 1.653  ppm dan 2.797 ppm, secara berurutan. Kesimpulan dari penelitian ini adalah ekstrakfraksi polar dari daun C. hystrix lebih toksik dibandingkan dengan  C. aurantifoliaterhadap larva nyamuk instar III.  


2019 ◽  
Vol 38 (4) ◽  
pp. 463
Author(s):  
Lisna Ahmad ◽  
Suryani Une ◽  
Yoyanda Bait

Traditional food products are no longer attractive to the public, so it needs innovation to create the product has a functional value. The aim of this research is to produce fortified cornmeal with kersen leaf extract which has high antioxidant activity and consumer demand of organoleptics. This research used completely randomized design with single factor at 6 treatment level, soaking grits of corn on cherry extract with concentration 0%, 10%, 20%, 30%, 40%, and 50%. The results of nutrient analysis consists protein ranged from 7.59% – 8.93%, fat 0.53% – 0.96%, ash content 0.05% – 0.12%, water content 11.6% – 13.37% and carbohydrate 77.24% – 78.91%. The result of antioxidant activity showed that the higher concentration of kersen leaf extract which added higher also the antioxidant activity of fortified cornmeal is 11.85 – 36.17%. Based on panelist organoleptic test give neutral response until somewhat like for the aspect of color, texture, aroma and overall grits while for panelist taste aspect give response rather dislike.


2012 ◽  
Vol 3 (1) ◽  
Author(s):  
Suharno Zen

Dengue fever is an infectious disease caused by a virus and is transmitted by the Aedes aegypti mosquito. Dengue fever has not yet found a cure and the only prevention is through mosquito vector  control. Health Department policy to prevent and eradicate dengue fever today is to make eradication by means of mechanical, physical, chemical or biological. Biological is one of alternative  control of mosquito vectors that are safe for humans and the environment but still effective in suppressing the mosquito vector. One attempt to do that is by using animals to fight other creatures, or better known as biological control. In the present research was performed using fish of Betta spp, Cyprinus carpio and Oreochormis niloticus as a natural predator of the Aedes aegypti mosquito. The design of this research is  Completely Randomized Design (CRD) with three treatments are 3 types of fish with 5 times repeated. Data were analyzed with analysis of the range and tested further with the Smallest Real Differences Test (LSD) on the real level 5%. The result of this research is the ability Betta spp eating is higher than the Oreochromis niloticus and Cyprinus carpio by the number of larvae that consumed between 34.6 to 36.9 mosquito larvae tail. The ability to eat at various types of fish against mosquito larvae, influenced by several factors: the agressive, espesialisasi fish to food, the active duration and the amount of time the fish is active in 24 hours.


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