scholarly journals In vitro evaluation of some halophilic bacterial isolates as biofertilizers

2020 ◽  
Author(s):  
E. A. Samsonova ◽  
I. M. Ibrahim ◽  
Yu. P. Fedonenko ◽  
S. A. Konnova
Keyword(s):  
Heavy Metal ◽  
Nitrogen Fixation ◽  
Halophilic Bacteria ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Bacterial Isolates ◽  
In Vitro Evaluation

For six strains of halophilic bacteria, nitrogen fixation ability, production of phytohormones and siderophores, the solubilization of phosphates, and heavy metal resistance were revealed.

scholarly journals Bioremediation of lead using bacterial isolates and study their plant relieving effect on wheat under metal contamination

2020 ◽  
Vol 35 (1-2) ◽  
Author(s):  
Prashakha J. Shukla ◽  
Vishwa R. Vyas
Keyword(s):  
Heavy Metal ◽  
Stress Condition ◽  
Inhibitory Concentration ◽  
Anthropogenic Activities ◽  
Wheat Plant ◽  
Cost Effective ◽  
Heavy Metal Resistance ◽  
Metal Resistance

Increasing concentration of heavy metals due to various anthropogenic activities is a serious problem. To overcome this issue, many chemical and physical methods are available but they are either directly or indirectly harmful to nature. By these methods more quality of chemicals are wasted. So, bioremediation is the best method to remove pollutants. It is an eco-friendly and cost-effective process. A low concentration of heavy metal is required to plant for their growth and metabolic process but at higher concentration, plants do not survive. With the use of microbes, we can survive plants at certain levels. During this work heavy metal tolerating microorganism was isolated and purified. Various tests were performed like staining, minimum inhibitory concentration, multiple heavy metal resistance, multiple antibiotic resistance, biochemical test, DNA isolation, in vitro examination of the wheat plant under the stress condition of lead (1000ppm).

scholarly journals Antibiotic Profiling of Heavy Metal Resistant Bacterial Isolates from the Effluent of a Garment Industry in Lalitpur, Nepal

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 203-206 ◽  
Author(s):  
M. Sharma ◽  
H.P. Thapaliya
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Heavy Metal Resistance ◽  
Garment Industry ◽  
Metal Resistance ◽  
Antibiotics Resistance ◽  
Bacterial Isolates ◽  
Heavy Metal Resistant ◽  
Multiple Antibiotics

Heavy metal resistant bacterial isolates from the effluent in a garment industry site were examined to assess their resistance towards multiple antibiotics. Heavy metal resistance property has been found to enhance the antibiotic resistance ability of microorganisms. Isolation of the heavy metal resistant organisms was done in media containing salts of heavy metals. Organisms were identified belonging to the genera Bacillus, Corynebacterium, Lactobacillus, Aeromonas and Enterococcus. Bacterial isolates were tested for their sensitivity to seven common antibiotics (penicillin, tetracycline, erythromycin, chloramphenicol, gentamicin, vancomycin and cotrimoxazole) using Kirby-Bauer technique. Isolates were found to be resistant to multiple antibiotics but all the isolates were sensitive to gentamicin. The data of our study indicates that metal pollution of the environment is the cause of heavy metal resistance isolates and hence antibiotic resistance.Key words: Heavy metal, effluent, antibiotics, resistance, Bacteria, pollution.DOI: 10.3126/on.v7i1.2572Our Nature (2009) 7:203-206  

scholarly journals Analysis of the Genome of the Heavy Metal Resistant and Hydrocarbon-Degrading Rhizospheric Pseudomonas qingdaonensis ZCR6 Strain and Assessment of Its Plant-Growth-Promoting Traits

2021 ◽  
Vol 23 (1) ◽  
pp. 214
Author(s):  
Daria Chlebek ◽  
Tomasz Płociniczak ◽  
Sara Gobetti ◽  
Agata Kumor ◽  
Katarzyna Hupert-Kocurek ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Plant Growth ◽  
Contaminated Soils ◽  
Growth Promotion ◽  
Sulfur Metabolism ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Minimal Inhibitory Concentrations ◽  
Plant Growth Promoting

The Pseudomonas qingdaonensis ZCR6 strain, isolated from the rhizosphere of Zea mays growing in soil co-contaminated with hydrocarbons and heavy metals, was investigated for its plant growth promotion, hydrocarbon degradation, and heavy metal resistance. In vitro bioassays confirmed all of the abovementioned properties. ZCR6 was able to produce indole acetic acid (IAA), siderophores, and ammonia, solubilized Ca3(PO4)2, and showed surface active properties and activity of cellulase and very high activity of 1-aminocyclopropane-1-carboxylic acid deaminase (297 nmol α-ketobutyrate mg−1 h−1). The strain degraded petroleum hydrocarbons (76.52% of the initial hydrocarbon content was degraded) and was resistant to Cd, Zn, and Cu (minimal inhibitory concentrations reached 5, 15, and 10 mM metal, respectively). The genome of the ZCR6 strain consisted of 5,507,067 bp, and a total of 5055 genes were annotated, of which 4943 were protein-coding sequences. Annotation revealed the presence of genes associated with nitrogen fixation, phosphate solubilization, sulfur metabolism, siderophore biosynthesis and uptake, synthesis of IAA, ethylene modulation, heavy metal resistance, exopolysaccharide biosynthesis, and organic compound degradation. Complete characteristics of the ZCR6 strain showed its potential multiway properties for enhancing the phytoremediation of co-contaminated soils. To our knowledge, this is the first analysis of the biotechnological potential of the species P. qingdaonensis.

scholarly journals Response of the callus cells of fir (Abies nordmanniana) to in vitro heavy metal stress

2017 ◽  
Vol 59 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Katarzyna Nawrot-Chorabik
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Heavy Metal Stress ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Stress Factors ◽  
Negative Effects ◽  
Abies Nordmanniana ◽  
Long Time

Abstract The aim of the presented research was to investigate the effect of three heavy metals - lead, cadmium and copper - on the callus cells of Abies nordmanniana. The toxicity degree and toxicity effect of the selected heavy metals was determined on the embryonic level. On the basis of the spectrometric analyses as well as macroscopic and microscopic observations, this research referred to the accumulation of heavy metals in tissues, assuming that this mechanism is related to the acquisition of tolerance by cells exposed to this type of abiotic stress. Moreover, the effect of the genotype of fir on the cell defence, that is, the induction of tolerance, was analysed. Understanding of the issues related to the heavy metal resistance of plant genotypes in future may contribute to the selection of genotypes of individuals that are more resistant to stress factors, particularly in the multi-directional and rational forest management. The results showed that lead (20 mg l-1), which proved to be the most toxic amongst the three examined heavy metals, has the most severe negative effects on the tissue of fir trees. Copper (20 mg l-1) was accumulated for a long time in the cells of fir trees, and it was not degraded or excreted outside the tissues even after three weeks of in vitro culture. Of the three tested genotypes, G14 had the greatest tendency to accumulate each of the examined metals, that is, it appeared to be the least tolerant genotype.

scholarly journals Identification Of Cu Resistant Bacteria From Tin Mining-Affected Sea Sediment

2020 ◽  
Vol 6 (2) ◽  
pp. 112-119
Author(s):  
Rahmad Lingga ◽  
Budi Afriyansyah
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Contamination ◽  
Heavy Metal Resistance ◽  
Negative Influence ◽  
Metal Resistance ◽  
Mining Activity ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Gram Staining

Tin mining activity at sea has a various negative influence on the environment. One of them is heavy metal contamination that can affect the life of fisheries biota. This research conducted to isolate and test the Cu heavy metal resistance of marine sediment bacteria that are affected by tin mining activity. Sediment sampling was carried out in the area of tin mining to the mudflat neared to the mangrove area. Bacterial isolation was carried out by spread plate method and bacterial characterization included cell shape, Gram staining and biochemical tests. Furthermore, bacterial isolates tested for resistance to metals with concentrations of 10 ppm, 20 ppm, 40 ppm, 80 ppm and 100 ppm. The results showed that bacterial isolates originating from marine sediments affected by mining activity were resistant to Cu heavy metal at various concentrations. Isolates B6, B8 and A10 showed the highest resistance up to a concentration of 100 ppm

scholarly journals Antibiotic and Heavy Metal Resistance of Bacteria Isolated from Diseased Mud Crab (Scylla serrata)

2015 ◽  
Vol 3 (3) ◽  
pp. 1-15
Author(s):  
Cik Syahrizawati M Z. ◽  
Ikhwanuddin M. ◽  
Wendy W. ◽  
Zulhisyam AK ◽  
Lee SW
Keyword(s):  
Heavy Metal ◽  
Vibrio Parahaemolyticus ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Edwardsiella Tarda ◽  
Scylla Serrata ◽  
Bacterial Isolates ◽  
Mud Crab ◽  
Salmonella Spp ◽  
Klebsiella Spp

A total of 320 bacteria isolated from marketable size and diseased mud crab (Scylla serrata) at a commercial farm. The isolated bacteria were Aeromonas spp. n = 70, Edwardsiella tarda n = 50, Vibrio alginolyticus n = 40, Vibrio parahaemolyticus n = 20, Salmonella spp. n = 70 and Klebsiella spp. n = 70. All the bacterial isolates were tested for antibiotic susceptibility against 16 types of antibiotics by using disk diffusion method. The antibiotics tested in this study were nalidixic acid (30 ?g/disk), oxolinic acid (2 ?g/disk), compound sulphonamides (300 ?g/disk), doxycycline (30 ?g/disk), tetracycline (30 ?g/disk), novobiocin (30 ?g/disk), chloramphenicol (30 ?g/disk), kanamycin (30 ?g/disk), sulphamethoxazole (25 ?g/disk), flumequine (30 ?g/disk), erythromycin (15 ?g/disk), ampicillin (10 ?g/disk), spiramycin (100 ?g/disk), oxytetracycline (30 ?g/disk), amoxycillin (25 ?g/disk) and fosfomycin (50 ?g/disk). Heavy metal resistance pattern of the present bacterial isolates was also characterized against mercury (Hg2+), chromium (Cr6+), copper (Cu2+), and Zinc (Zn2+) by using two fold agar dilution method. The percentage of antibiotic sensitivity of the present bacterial isolates was ranged from 12.5 % to 100 % in which most of the present bacteria isolates were not sensitive to ampicillin whereas all the bacteria isolates were sensitive to nalidixic acid, flumequine and oxytetracycline. Overall, the total of antibiotic sensitive case was reported as 72.7% whereas antibiotic resistance and intermediate sensitive case was recorded as 19.7% and 7.4 %, respectively. The Multiple Antibiotic Resistance (MAR) values were range of 0.03 to 0.29 in which Aeromonas spp (0.29) showed the highest value of MAR. This was followed by Salmonella spp. (0.21) and Klebsiella spp. (0.21), Edwardsiella tarda (0.20), Vibrio alginolyticus (0.09) and Vibrio parahaemolyticus (0.03). The MAR value indicated that the commercial S. serrata were not contaminated to the test antibiotics. Furthermore, low resistance activity of the present bacterial isolates to the tested heavy metals (Cr6+: 20.7 % to 30.8 %, Zn2+: 0 % to 40 %, Cu2+: 18.8 % to 25 % and Hg2+: 30 % to 33.3 %) was observed.

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