scholarly journals Progesterone and estrogen levels in Awassi ewes treated with vaginal sponges

2013 ◽  
Vol 12 (1) ◽  
pp. 183
Author(s):  
A.A. Omar
Keyword(s):  
Veterinary Medicine ◽  
Jugular Vein ◽  
Treated Group ◽  
Control Group ◽  
Estrus Synchronization ◽  
Blood Samples ◽  
Elisa Technique

This study was conducted on10 healthy Iraqi Awassi ewes that examined by ultrasonography technique, with ages between 2 to 3 years, presented at the farm of the College of Veterinary Medicine, Al-Anbar University, Fallujah, during the period from the end of the February until the half of the March 2012. Vaginal sponges saturated with 40mg Flurogestone acetate were inserted in the vagina of each tested animals in order to induce estrus For synchronization for 14 days, at day 13of the experiment the animals were injected i.m. by Equine chorionic gonadotropine eCG (500 i.u.) then the sponges were removed and the animals injected i.m. with (200 i. u.) of Human chorionic gonadotropine (HCG). Blood samples were collected from jugular vein with vacutainer tubes each two days until the experiment was complete. Serum was collected after centrifugation with a speed 3000R/m for 10 minutes. Values of estrogen and progesterone hormones in serum were estimated by Enzyme Linked Immuno Sorbent Assay (ELISA) technique. Values of the estrogen and progesterone in the treated animals were (1.1932 ± 0.02380), (0.9558 ± 0.3282) respectively, while the levels of the two hormones for the control group was (1.1138 ± 0.02295) for the estrogen and (0.8702 ± 0.0152) for the progesterone. There was a significant different between the level of estrogen and progesterone hormones P < 0.05 in a treated group as a compare with the control group. It was concluded from this study that estrus synchronization of the ewes with vaginal sponge affected the values of estrogen and progesterone hormones in the serum

Serum Endocrine Profile in Relation to Estrus Synchronization in Surti Does

2018 ◽  
Vol 13 (03) ◽  
Author(s):  
M. M. Chaudhary ◽  
C. T. Khasatiya ◽  
S. B. Patel ◽  
S. S. Chaudhary ◽  
V. B. Atara ◽  
...  
Keyword(s):  
Jugular Vein ◽  
Estrus Synchronization ◽  
Progesterone Concentration ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Group Iii ◽  
Group I ◽  
Endocrine Profile ◽  
Group Ii ◽  
And Control

The serum progesterone and estradiol profiles during synchronization of estrus by buck effect and PGF2α treatments were monitored in Surti does. Total eighteen non-pregnant does selected were evenly divided into 3 groups, 6 does in each group. The does of Group I were teased with a sexuallyactive- apronized buck; and those of Group II were treated with PGF2α, i.e., Inj. Lutalyse® @ 7.5 mg/doe IM twice 11 days apart, while the Group III served as untreated control. Blood samples were collected from all the animals on day 0 (before 1st PGF2α injection), 3rd day (during treatment), 11th day (before 2nd PGF2α injection), 14th day (after treatment) and 40th day (post-service) by jugular vein puncture. The serum separated was stored at -20°C till further analysis. In all the three groups, 83.33% does, conceived at first service in the sampling cycle. The overall mean serum progesterone concentration of Group I does (5.82±0.72 ng/ml) was significantly higher (p less than 0.01) as compared to Group II (2.93±0.38 ng/ml) and III (2.88±0.30 ng/ml). Similarly, the overall mean serum progesterone concentration of Surti does on day 0 (2.65±0.46 ng/ml), 3rd (2.56±0.80 ng/ml), 11th (4.45±0.84 ng/ml) and 14th (3.40±0.63 ng/ml) did not differ significantly, but the overall mean level at day 40 (6.31±0.45 ng/ml) was significantly (p less than 0.01) higher, because most of animals became pregnant at that time. The overall mean serum oestradiol-17β levels of Group I (24.40±2.98 pg/ ml) was significantly higher (p less than 0.01) than in Group II (15.77±1.77 pg/ml) and III (12.21±1.45 pg/ ml). On the other hand, the overall mean serum oestradiol-17β levels of Surti does on day 0 (12.89±1.21 pg/ml), 3rd (15.84±1.74 pg/ml), 11th (14.81±1.96 pg/ml), 14th (22.15±2.97 pg/ml) and 40th (21.64±5.16 pg/ml) did not differ significantly (p>0.05) and the slightly higher overall mean level found at 40th day might be the influence of the non-pregnant does at first service in the cumulative animals. The hormonal profile reflected the initiation of cyclicity and establishment of pregnancy in treated and control animals.

scholarly journals Progesterone Levels During Pregnancy in Ewes Treated with Bone Marrow Stromal Cells

2019 ◽  
Vol 12 (2) ◽  
Keyword(s):  
Bone Marrow ◽  
Beneficial Effect ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Jugular Vein ◽  
Marrow Stromal Cells ◽  
Control Group ◽  
Blood Samples ◽  
Significant Difference ◽  
Equine Chorionic Gonadotropin

This study was conducted to determine the effect of bone marrow stromal cells (BMSCs) on progesterone level during pregnancy in ewes. Flurogestone Acetate Sponges 40 mg, followed by 400 i.u. Equine chorionic gonadotropin (eCG) were used to synchronize estrus and ovulation. The animals were divided into three equal groups (5 animals for each group). The 1st and 2nd group injected intravenously after eCG injection with 1x108 and 2x108 respectively with BMSCs while the 3rd group was injected with normal saline which serve as a control group. Blood samples were collected during pregnancy, at day 10, 21, 85 from the jugular vein. At day 10, 21 the results showed that there was a significant difference (p≤0.05) in the level of progesterone between treated groups as compared with the control group. While there was no significant difference between different groups at day 85. It was concluded from this study that BMSCs have a beneficial effect in ewe’s reproductive system, by increasing the level of progesterone at early pregnancy.

Different wavelengths of light on GnRH mRNA, LH, steroid hormones and egg lay in the ageing hens (Gallus gallus domesticus).

2016 ◽  
Vol 5 (06) ◽  
pp. 4597
Author(s):  
Janardhan Reddy Ippala* ◽  
Ashish Mishra ◽  
Mondal S. ◽  
David G. C. ◽  
Ravi Kiran G. ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Egg Production ◽  
Treated Group ◽  
Reproductive Period ◽  
Control Group ◽  
Blood Samples ◽  
Egg Formation ◽  
High Concentrations ◽  
Normal Spectrum ◽  
And Control

The aim of this study was to investigate the effects of red spectrum of light (650nm, treated n=12) and normal spectrum of light (450nm control=12) on circulating concentrations of luteinizing hormone (LH), progesterone (P4), estradiol (E2β), GnRH mRNA, pause days and egg production in birds later in the reproductive period from 92-102weeks of age. Twenty-four White Leghorn birds of same age group were divided into two groups of 12 in each as control and treated. Birds in the control group were exposed to normal spectrum of light (450nm of length) and birds in the treated group were exposed to red spectrum of light (650nm, treated n=12). Egg production and inter sequence pauses were recorded daily from both the groups. Plasma LH, E2β and P4 concentrations were estimated in blood samples collected at weekly intervals. At 97th weeks of age, blood samples from treated and control birds were obtained every 3 h for 36 h to study the surges of LH. It was found that plasma GnRH was higher (p < 0. 01) in treated birds with high concentrations of LH, its 3 h LH surges, E2β and P4 in plasma. Higher egg production, less pause days in treated birds may be the result of high GnRH associated with positively correlated responses of high concentrations of LH (with regular interval and duration of LH surges), E2β and P4 concentration required for completion of egg formation and oviposition. In conclusion, red spectrum of light enhanced GnRH mRNA (p < 0. 01), increased (p < 0. 01) steroid hormones and LH surges, for egg formation and oviposition and enabled the birds to lay more eggs even later in the productive period with the available resources under normal husbandry practices. 

A study of the luteolytic mechanism of the antiprogesterone RU486 during the late-luteal phase in pseudopregnant rats

1996 ◽  
Vol 150 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Y Nakamura ◽  
H Tamura ◽  
M Ono ◽  
K Shimamura ◽  
N Sugino ◽  
...  
Keyword(s):  
Subcutaneous Injection ◽  
Luteal Phase ◽  
Treated Group ◽  
Sesame Oil ◽  
Serum Prolactin ◽  
Control Group ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Serial Blood ◽  
Late Luteal Phase

Abstract The purpose of this study was to examine the possible mechanism through which RU486 induces luteolysis during the late-luteal phase in pseudopregnant (PSP) rats. PSP rats received a subcutaneous injection of RU486 in sesame oil (5 mg/kg body weight) or sesame oil alone once a day between day 9 and day 11 of pseudopregnancy. Serial blood samples were collected on days 5, 9, 10, 11 and 12 and assayed for progesterone content. To examine the possible action of RU486 through a uterine and/or a pituitary (prolactin-dependent) mechanism, PSP rats and chronic hysterectomized PSP rats which had been hysterectomized before PSP induction received a subcutaneous injection of RU486 in sesame oil (5 mg/kg body weight), sesame oil alone, prolactin in 50% polyvinylpyrrolidone (15 IU/day), or RU486 and prolactin once a day between day 9 and day 11 of pseudopregnancy. Serial blood samples were collected on days 5, 9, 10 and 11 and assayed for progesterone content. Blood samples were also collected at 0400 h on day 12 and used for prolactin and progesterone determinations. To examine the direct effect of RU486 on corpus luteum and/or pituitary, hysterectomized rats underwent hypophysectomy and pituitary autotransplantation on dioestrus 1 and received a subcutaneous injection of RU486 in sesame oil or sesame oil alone for 3 days between day 21 and day 23 after surgery. Serial blood samples were collected on days 10, 21, 22, 23 and 24 and assayed for progesterone and prolactin contents. In ordinary PSP rats, serum progesterone levels were significantly (P<0·01) lower in the RU486-treated group than in the control group (9 ± 1 vs 53 ± 7 ng/ml; mean ± s.e.m.) on day 11. Serum prolactin levels at 0400 h on day 12 of pseudopregnancy were significantly (P<0·05) lower in the RU486-treated group than in the control group (16 ±4 vs 154 ±44 ng/ml; mean ± s.e.m.). The concomitant prolactin treatment reversed the luteolytic effects of RU486 on day 11 of pseudopregnancy. In hysterectomized PSP rats, RU486 also suppressed serum prolactin levels, and the concomitant prolactin treatment again reversed the luteolytic effects of RU486. In hysterectomized rats which were hypophysectomized and pituitary autotransplanted, RU486 treatment did not induce any significant changes in serum progesterone and prolactin levels. These results indicated that RU486 induced luteolysis during the late-luteal phase in PSP rats by suppressing prolactin secretion via a hypothalamic mechanism. Journal of Endocrinology (1996) 150, 93–98

scholarly journals The Effect of Enteritis, Pneumonia and Omphalitis on Oxidative/Antioxidant Balance in the Calves

2019 ◽  
Vol 7 (3) ◽  
pp. 539
Author(s):  
İbrahim Yurdakul ◽  
Ugur Aydogdu
Keyword(s):  
Nitric Oxide ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Oxidative Damage ◽  
Jugular Vein ◽  
Control Group ◽  
Antioxidant Treatment ◽  
Blood Samples ◽  
Neonatal Disease

In this study, it was aimed to determine the effects of enteritis, pneumonia and omphalitis on oxidative/antioxidant balance in the calves. In total, 27 calves with neonatal disease and 10 healthy calves (control) were used. The sick calves were divided into 3 groups according to localization of infection, omphalitis (n = 10), pneumonia (n = 8) and enteritis (n = 9). Blood samples of the calves were taken from jugular vein for analysis. Blood in tubes without anticoagulant was centrifuged at 5000 rpm for 5 min following clotting, and the sera were removed and stored at -80°C until analyzed. Serum malondialdehyde and nitric oxide levels of all groups were significantly higher, while glutathione, glutathione peroxidase, catalase and superoxide dismutase levels were significantly lower compared to the control group. As a conclusion, diarrhea, pneumonia and omphalitis are caused by oxidative damage in the calves, and antioxidant treatment may be beneficial in the treatment of these diseases.

Effect of Tylvalosin on Blood Parameters in Healthy Broiler Chickens

2021 ◽  
Vol 19 (8) ◽  
pp. 141-144
Author(s):  
Mohammed Mosleh Shwaish ◽  
Ammar H. Salman ◽  
Asif Hasan Abdulrazaq
Keyword(s):  
Body Weight ◽  
Normal Saline ◽  
Broiler Chickens ◽  
Treated Group ◽  
Blood Parameters ◽  
Control Group ◽  
Broiler Chicks ◽  
Blood Samples

To evaluate the effects of Tylvalosin on blood parameters in healthy broiler chickens, twenty broiler chicks (Ross 308) aged 21 days old and weight 600-800 gm was divided into two equal groups, the first one was control group (G1) and the second one was treated group (G2). G1 dosed with 1ml/kg of body weight of normal saline while G2 dosed with 25mg/Kg of body weight of Tylvalosin. Blood samples were collected with EDTA tubes after 4 days of treatment. The results showed significant decrease in total WBCs counts in G2 treated group as compared with control group (G1), also the eosinophil showed a significant decrease in G2 as compared with G1, while other parameters didn’t show any significant differences. In conclusion, WBCs were decreased after treatment with tylvalosin.

scholarly journals Effect of Adiponectin Hormone on Serum Lipoproteins Levels in Female Rats

2013 ◽  
Vol 12 (2) ◽  
pp. 50
Author(s):  
H, A. A. Al-Baka`a
Keyword(s):  
Normal Saline ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Serum Lipoproteins ◽  
Control Groups ◽  
Dose Administration ◽  
Blood Samples ◽  
And Control

This investigation aimed to study the effect of adiponectin hormone on serum lipoproteins levels in female rats. For this purpose, thirteen female rats were divided into treated and control groups. Each animal in the treated group received a daily intraperitoneal 25microgram does of adiponectin dissolved in 0.2ml normal saline for fifteen respective days. In the same time, each animal in the control group received a daily intraperitoneal 0.2ml normal saline for fifteen respective days. The animal has fasted for 12 hours after last dose administration. Blood samples were collected, and the sera obtained. Analysis showed a significant increase in the serum HDL level and a significant decrease in LDL concentration. AP < 0.05was considered significant.

scholarly journals Effect of Coriander Seeds (Coriandrum Sativum) on Wool Growth and It's Traits and Some Haematological Traits of Awassi Ewes

2010 ◽  
Vol 34 (2) ◽  
pp. 148-155
Author(s):  
Drgham H. Yousif AL-Zwein
Keyword(s):  
Jugular Vein ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Wool Fiber ◽  
Feed Additive ◽  
Control Group ◽  
Blood Samples ◽  
Wool Growth ◽  
Physical Traits ◽  
The Right

This study was conducted to find out the effect of coriander seeds as feed additive on some hematological traits and wool growth and it's physical traits of Awassi ewes, blood samples were taken biweekly for blood picture, and wool samples were taken every 2.5 months for wool growth and its physical traits. Eighteen Awassi ewes at the age of 2-3 years were randomly divided equally into three groups (6each ) "body weight was considered".Ewes in the first group kept as control and daily fed on concentrate diet (350 g/ head), while the second group was fed on the same diet contain (2.5%) of coriander seeds, while the third group was fed on same diet contain (5%) of coriander seeds. Also all animals were allowed to graze. Blood samples were taken from jugular vein for blood traits viz (PCV) packed cell volume, (Hb) hemoglobin, (MCHC) mean cell hemoglobin concentration. Wool samples (5×5) cm2 were clipped from the last six ribs of the right chest for measuring wool growth and its traits (creasy and clean wool, samples weights, clean wool ratio, staple and fiber lengths and diameters and wool fiber crimps in 1cm of length). The results of blood parameters revealed that the second and third groups were significantly (p<0.05) recorded higher values in their PCV ,Hb, MCHC during most studied periods compared with the control group ,While the second and third groups were significantly (P<0.05) recorded higher values than the first group (control) in their creasy and clean wool, fiber length, wool fiber crimps, while no differences where existed in other wool physical traits.

scholarly journals BIOCHEMICAL AND HAEMATOLOGICAL BIOMARKER DETERMINED IN FEMALE ALBINO RATS INDUCED WITH INFERTILITY AND ADMINISTERED WITH ANTHOCLIESTA VOGELII

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Olugbenga Oladimeji ◽  
O.A Lawal ◽  
A Steve
Keyword(s):  
Vitamin E ◽  
Hematological Parameters ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Traditional Use ◽  
Blood Samples ◽  
Biochemical Evaluation ◽  
Automated Hematology Analyzer

Anthocleista vogelii was investigated for the acclaimed fertility enhancing properties especially in females. The phyto-chemistry of the ethanolic leave extract of Anthocleista vogelii was determined prior to it being administered orally for 14 days to Wister albino rats. Micronor (norethisterone) or N-acetylcysteine (NAC) was given orally to induce temporary infertility in the female rats for seven (7) days prior to other treatment. The Blood samples from experimental animal groups were collected through cardiac puncture when the rats were sacrificed after the completion of fourteen (14) days extract administration. The vitamin E analysis was performed using HPLC. The hematological parameters were performed using the Sysmex® Automated Hematology Analyzer KX-21N. Biochemical evaluation of glutamic pyruvate transaminase ALT, alkaline phosphatase ALP, glutamic oxaloacetate transaminase AST, total cholesterol and total triacylglycerol was done using randox biochemical kits. The extract was found to possess Anthraquinones, Terpenoids, Flavonoids, Saponins, Alkaloids, Phenols and Phytosterols. The hematological parameters showed a significant increase (P<0.05) in absolute middle cells (basophils, eosinophils and monocytes) count. It also showed a significant reduction in the ALP, ASP, AST, TAG and cholesterol level (p<0.05). The obtained results showed a significant increase of vitamin E concentration in the female rats in the control group compared to extract treated group. The result also suggest that Anthocleista vogelii may have a role in creating the environment required for enhancing pregnancy with the Vitamin E concentration production that support the claims on the traditional use of Anthocliesta vogelii to enhance fertility in female.

scholarly journals Oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ADE vitamin complex

2013 ◽  
Vol 33 (suppl 1) ◽  
pp. 58-62 ◽  
Author(s):  
Rebeca A. Weigel ◽  
Alessandra Silva Lima ◽  
Aline Alberti Morgado ◽  
Giovanna Rocha Nunes ◽  
Maurício M. Miriam ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Metabolism ◽  
Energy Demand ◽  
Energy Use ◽  
Treated Group ◽  
Control Group ◽  
Blood Samples ◽  
Lactate Levels ◽  
Aerobic And Anaerobic ◽  
Variable Energy

Horses used for the game of polo experience abrupt and frequent changes in exercise intensity. To meet this variable energy demand, the horses use both aerobic and anaerobic pathways in varying proportions and intensities. In this context, there must be a balance between the formation of reactive oxygen species (ROS) and the action of antioxidants to prevent oxidative stress and its consequences. The effect of supplementation with an ADE vitamin complex on oxidative metabolism was evaluated in 18 crossbred horses randomly divided between a treated group (TG) and a control group (CG). The TG animals received the ADE vitamin complex (1mL/50 kg of body weight) by deep intramuscular injection at 30 and 15 days before the game. The CG horses received 10ml of saline by the same administration route and schedule. During the polo match, the animals played for a total of 7.5 min. Blood samples were collected on the same days as the treatments were administered, and immediately before and at 15, 90 and 180 minutes after the game. The concentrations of creatine phosphokinase (CK), lactate dehydrogenase (LDH), lactate, glucose, aspartate aminotransferase (AST), glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in the blood samples. After the game, the TG demonstrated higher levels of AST, lactate and glucose than the CG, suggesting more efficient energy use by the treated animals. The higher GSH and lower lactate levels in the TG before the game suggest the presence of a greater antioxidant supply in the treated animals. The maintenance of the MDA levels indicates that neither of the groups exhibited oxidative stress.

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