scholarly journals BIOCHEMICAL AND HAEMATOLOGICAL BIOMARKER DETERMINED IN FEMALE ALBINO RATS INDUCED WITH INFERTILITY AND ADMINISTERED WITH ANTHOCLIESTA VOGELII

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Olugbenga Oladimeji ◽  
O.A Lawal ◽  
A Steve
Keyword(s):  
Vitamin E ◽  
Hematological Parameters ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Traditional Use ◽  
Blood Samples ◽  
Biochemical Evaluation ◽  
Automated Hematology Analyzer

Anthocleista vogelii was investigated for the acclaimed fertility enhancing properties especially in females. The phyto-chemistry of the ethanolic leave extract of Anthocleista vogelii was determined prior to it being administered orally for 14 days to Wister albino rats. Micronor (norethisterone) or N-acetylcysteine (NAC) was given orally to induce temporary infertility in the female rats for seven (7) days prior to other treatment. The Blood samples from experimental animal groups were collected through cardiac puncture when the rats were sacrificed after the completion of fourteen (14) days extract administration. The vitamin E analysis was performed using HPLC. The hematological parameters were performed using the Sysmex® Automated Hematology Analyzer KX-21N. Biochemical evaluation of glutamic pyruvate transaminase ALT, alkaline phosphatase ALP, glutamic oxaloacetate transaminase AST, total cholesterol and total triacylglycerol was done using randox biochemical kits. The extract was found to possess Anthraquinones, Terpenoids, Flavonoids, Saponins, Alkaloids, Phenols and Phytosterols. The hematological parameters showed a significant increase (P<0.05) in absolute middle cells (basophils, eosinophils and monocytes) count. It also showed a significant reduction in the ALP, ASP, AST, TAG and cholesterol level (p<0.05). The obtained results showed a significant increase of vitamin E concentration in the female rats in the control group compared to extract treated group. The result also suggest that Anthocleista vogelii may have a role in creating the environment required for enhancing pregnancy with the Vitamin E concentration production that support the claims on the traditional use of Anthocliesta vogelii to enhance fertility in female.

scholarly journals SOME IMMUNE FACTORS AND HORMONES DETERMINED IN FEMALE ALBINO RATS INDUCED WITH INFERTILITY AND ADMINISTERED WITH ANTHOCLIESTA VOGELII

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Olugbenga Oladimeji
Keyword(s):  
Test Group ◽  
Ethanolic Extract ◽  
Treated Group ◽  
Female Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Albino Rats ◽  
Traditional Use ◽  
Clusters Of Differentiation ◽  
Automated Technique

Anthocleista vogelii Planch, phyto-constituents was evaluated and the plant leave extracts investigated on the claims of the traditional medicine practitioners of its usage as fertility enhancer in females. Ethanolic extract of Anthocleista vogelii were administered orally for 14 days to female albino rats placed in different groups. First, temporary infertility was induced with Micronor (norethisterone) or Nacetylcysteine (NAC) given orally to some rats, for seven (7) days prior to other treatment. The rats were sacrificed after the completion of extract administration. The absolute counts of clusters of differentiation CD4+ and CD8+ was performed on the blood samples using the Becton Dickinson’s (BD) FACS Count Automated technique. Hormonal analysis was performed on sera obtained from the experimental animals using commercial standard enzyme-linked immunosorbent assay kit. The extract was found to possess Anthraquinones, Terpenoids, Flavonoids, Saponins, Alkaloids, Phenols and Phytosterols . The obtained results of the test group compared with control showed a statistically significant decrease (P<0.05) in CD4+ and CD8+ counts cells and Prolactin, testosterone respectively. The results showed a significant increase of estradiol, leutinizing hormone, in the female rats in the control group compared to extract treated group. The result also suggested that Anthocleista vogelii may have a role in creating the environment required for enhancing pregnancy by decreasing ratio of CD4+ and CD8+ linked Th1 andTh2 cytokines production activation. Estradiol, luetinising hormone, concentration therefore support the claims on the traditional use of Anthocliesta vogelii that it enhance fertility in female.

scholarly journals Ameliorative Effects of Fenugreek Seeds and Curcumin on Hematotoxicity induced by Nicotine in Male Albino Rats

2022 ◽  
Vol 3 (1) ◽  
pp. 01-08
Author(s):  
Azab Elsayed Azab ◽  
Mohamed Omar Albasha ◽  
Manal Abuelkasem Elnaif
Keyword(s):  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Group Iv ◽  
Control Group ◽  
Albino Rats ◽  
Group V ◽  
Fenugreek Seeds ◽  
Group Iii ◽  
Group I

The present study aimed to investigate the ameliorative effects of fenugreek seeds and curcumin on hematotoxicity induced by nicotine in male albino rats. 30 male F-344/NHsd Fischer rats, weighing from 180 to 200g were used in the present study. The animals were divided into five groups (6 rats for each); Group I (control group), Group II (nicotine treated group), Group III (nicotine/fenugreek seeds co-administered), Group IV (nicotine/curcumin co-administered), and Group V (nicotine/curcumin& fenugreek seeds co-administered). At the end of the experimentation and 24 hours after the last dose, all animals were anaesthetized with ether and blood samples were collected by heart puncture. The samples were collected in clean dry tubes containing the anticoagulant substance EDTA and used for the hematological studies. The results showed that the animals treated with nicotine for 4 weeks showed a significant decrease in RBCs count, hemoglobin concentration, hematocrit value, MCH, MCHC, and platelets count, and increased MCV and WBCs count as compared to the control group. Co-administration of nicotine with fenugreek and/or curcumin caused improvement in all hematological parameters when compared with nicotine group. It can be concluded that nicotine had a strong effect on the hematological parameters. The ingestion of fenugreek and/or curcumin prevent the hematoxicity induced by nicotine. The current study suggests that fenugreek and curcumin may be useful in combating free radical-induced hematotoxicity induced by nicotine.

scholarly journals Cellular Damage After Prolonged Low-Dose Exposure of Neonicotinoid in Rats

2021 ◽  
Author(s):  
Rahat Naseer ◽  
Affan Tariq ◽  
Munazza Raza Mirza ◽  
Muhammad Rashid ◽  
Syed Qasim Raza ◽  
...  
Keyword(s):  
Vitamin E ◽  
Low Dose ◽  
Prolonged Exposure ◽  
Cell Damage ◽  
Hematological Parameters ◽  
Treated Group ◽  
Alpha Tocopherol ◽  
Cellular Damage ◽  
Control Group ◽  
New Class

Abstract Background; Dinotefuran is a new class of neonicotinoids claimed to be harmless to mammals and humans. This claim was daunted by the documented effect of dinotefuran on honeybees and further studies were required. Aim: The study was designed to assess the capaciousness of damage caused by prolonged exposure of dinotefuran in mammals and probable strategy to neutralize its effect. Methodology: Ninety-day trial using Wistar rats (n=45) was conducted while dividing them into three groups: untreated control group, insecticide (dinotefuran) treated group, and dinotefuran treated and vitamin E supplemented group. Dinotefuran was administrated orally (LD25). Vitamin E (alpha-tocopherol) supplementation was given in water ad libitum. Blood sampling was done twice a month, and hematological and biochemical data were recorded. After expiry of trial period, the experimental rats were anesthetized and sacrificed. Organs (kidneys, liver, and heart) were isolated from each groups, weighed, and stored at approximately -20°C till further processing, analysis and histopathology were performed. Results: All the hematological parameters were affected significantly. Histopathology of tissues showed clear necrosis in all the tissues except kidneys. All the biomarkers of oxidative stress and comet assay demonstrated significant cell damage. All the parameters showed improvement after vitamin E supplementation but non-significantly. Significance: These findings were suggestive that even low dose persistent exposure can lead to mutagenicity and carcinogenicity in mammals and other non-target species hence revised policy guidelines and more intelligent use of these chemicals is required.

scholarly journals Effect of wheatgrass biscuits on hematology of wistar albino rats

2020 ◽  
Vol 15 (2) ◽  
pp. 50-55
Author(s):  
Suneetha Runjala ◽  
Y.L.N. Murthy
Keyword(s):  
Hematological Parameters ◽  
Good Deal ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Gram Positive ◽  
Potential Health ◽  
Group 2 ◽  
Pellet Form

The exploitation of herbal -based inhibitor compounds in food stuffs and medicine are gaining a good deal of interest due to potential health advantages. Recent researchers have attempted the exploration of traditional and plant-based therapeutical and medicinal preparations using bio-chemical analyses and in-vitro experimentation. The effect of wheatgrass biscuits on hematological parameters were studied in Wistar albino female rats for 21 days. The wheatgrass powder 20, 30 and 40 g per 100g incorporated biscuits prepared into pellet form labelled as V2, V3 and V4 formula supplemented to 3 sample groups normal laboratory diet to 1 control group under laboratory conditions. The average mean food efficiency during 21 days of supplementation recorded that control group has the highest efficiency food intake than sample groups. Group 3 has the lowest efficiency intake fed with V4, than group 1 and group 2 fed with V2 and V3. The results showed significant increase in RBC, WBC, Hematocrit, MCV and decreased levels of MCH in all groups. At the end of the study microflora contamination was estimated by using fecal collection of rats. Lactobacilli colonies were 6 to 7cfu/g were identified as creamy white and small colonies on lactobacilli as Gram positive on MRS agar plates, which are under permissible limits. Less bacteria indicates that rats are not affected by any food poising during 21 days. E. coli colonies exhibited a metallic sheen on Sabouraud’s agar medium and counted 2 to 3 cfu/g appeared as shiny white colonies as gram negative. Yeast appears as gram-positive globules. It was found that the wheatgrass biscuits effectively improved normal haematological and physical body parameters without signs of 1any abnormalities.

scholarly journals Edible Mushroom significantly ameliorated Hepatorenal Toxicity of Butyl Paraben (BP) in Albino Rats.

2022 ◽  
Author(s):  
Kassahun berhane
Keyword(s):  
Plasma Proteins ◽  
Edible Mushroom ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Kidney Function Tests ◽  
Vascular Congestion ◽  
Liver And Kidney ◽  
Kidney Functions

Abstract Introduction: Parabens are used commonly as preservatives in a range of cosmetics applied to the under arm and breast area as well as popular preservatives because of their cost.Aim of the work: This study was done to evaluate the neprohepatic toxicity of parabens. Materials and methods: Thirty adult female rats were used and given paraben orally for six months at parabens at dose of 10 % of the LD50 equal to 4.6mg\kg.bw. Mushroom was given orally to at dose of 10 mg/kg/day for six months too. Results: Oral administration of BP induced biochemical and histopathological changes. Biochemical changes: BP toxicity manifested by changes in the liver and kidney function tests manifested by increase AST, ALT, Bilirubin, urea and createnine with decreases to plasma proteins in comparison to control group. Giving mushroom caused amelioration to the nephrohepatic toxicity by inducing recovery in liver and kidney functions in comparison to paraben treated group. For histopathological findings: BP induced vascular congestion in liver and kidney in association with necrotic changes in the hepatorenal epithelium which improved after mushroom treatment. Conclusion: BP induced hepatorenal toxicity which improved by mushroom treatment.

scholarly journals Effect of Adiponectin Hormone on Serum Lipoproteins Levels in Female Rats

2013 ◽  
Vol 12 (2) ◽  
pp. 50
Author(s):  
H, A. A. Al-Baka`a
Keyword(s):  
Normal Saline ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Serum Lipoproteins ◽  
Control Groups ◽  
Dose Administration ◽  
Blood Samples ◽  
And Control

This investigation aimed to study the effect of adiponectin hormone on serum lipoproteins levels in female rats. For this purpose, thirteen female rats were divided into treated and control groups. Each animal in the treated group received a daily intraperitoneal 25microgram does of adiponectin dissolved in 0.2ml normal saline for fifteen respective days. In the same time, each animal in the control group received a daily intraperitoneal 0.2ml normal saline for fifteen respective days. The animal has fasted for 12 hours after last dose administration. Blood samples were collected, and the sera obtained. Analysis showed a significant increase in the serum HDL level and a significant decrease in LDL concentration. AP < 0.05was considered significant.

scholarly journals Hepatoprotective effect of Alchornea cordifolia leaf on liver damage in albino rats

2014 ◽  
Vol 2 (2) ◽  
pp. 217-221 ◽  
Author(s):  
J. M. Jacob ◽  
M. T. Olaleye ◽  
J. A. O. Olugbuyiro
Keyword(s):  
Vitamin E ◽  
Liver Damage ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Antioxidant Agents

Ethnopharmacological relevance: The dry leaf of Alchornea cordifolia (AC) is used, in traditional medicine in the S Nigeria, for the preparation of blood tonic, remedies for urinary, respiratory, liver and gas intestinal disorders. Aim of the study: This study investigated the protective property of AC leaf against liver damage in animals with a view to exploring its use for the treatment of hepatotoxicity in humans. Material and methods:  Ethanol extract of A. cordifolia was used to study the hepatoprotective activity in acetaminophen-induced Albino rats (150-200g). Animals in Group 1 served as vehicle control, Group 2 served as hepatotoxin (Acetaminophen 2g/kg treated) group, Groups 3 and 4 served as positive control (Vitamin E and Curcumin 100 mg/kg bw respectively) groups, and Groups 5-8 served as (200-500mg/kg bw) AC leaf extract treated groups while Group 9 served as normal group (AC extract only 300 mg/kg bw). Results: The hepatotoxic group showed hepatocytic necrosis, cellular infiltration and inflammation in the liver. The treatment group restored the liver cells to their normal lobular architecture in a dose dependent manner. The protection offered by the plant extract compared well with the standard antioxidant agents (Curcumin and Vitamin E). Tannins, flavonoids, alkaloids and saponins were detected in the phytochemical screening. Conclusion: Our findings suggest Alchornea cordifolia ethanol leaf extract as promising herpatoprotective herb and give credence to the folkloric use of this plant for the treatment of liver problems.DOI: http://dx.doi.org/10.3126/ijasbt.v2i2.10473Int J Appl Sci Biotechnol, Vol. 2(2): 217-221 

scholarly journals Hematological and Biochemical parameters study of female albino rats treated with lamotrigine drug

2019 ◽  
Vol 24 (3) ◽  
pp. 23
Author(s):  
Gawhar Ahmed Shekha ◽  
Kalthum Asaaf Maulood
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Haemoglobin Concentration ◽  
Chloride Ion ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Anti Epileptic Drug ◽  
Hematological And Biochemical Parameters

The present study was aimed to investigate the possible effects of the anti-epileptic drug lamotrigine (LTG) on some haematological and biochemical parameters in adult female rats. Forty-eight female rats were divided into three groups (each group=16). Group one can be considered as a control group, group two and three administrated lamotrigine drugs orally at a dose of 3.57mg/kg body weight and 7.14mg/kg body weight for 7,14,21,28 day and all groups fed with standard rat feed. The results showed that there were significant (P≤0.05) changes in haematological parameters in group two and three when compared with the control group during all period except the mean level of corpuscular haemoglobin concentration (MCHC). The liver enzyme aspartate transaminase (AST) and alanine transaminase (ALT) and serum urea, creatinine with calcium, potassium, sodium and chloride ion showed significant alteration in the treated group, the relative organ weight showed significant changes in group two and three in comparison with control group during 7,14,21 and 28 days. Estradiol level in group three increased at 7, 14 and 21 day and decreased at 28 days of treatment when compared with group two and the control group. This study suggested that treatment of healthy female albino rats with therapeutic doses of lamotrigine drug for 28 days generally affect on included parameters in this study.   http://dx.doi.org/10.25130/tjps.24.2019.044

In Vivo Anti-Anemic Effect of an Aqueous Root Extract of Phyllanthus Muellerianus (Kuntze) Exell in Model Rats.

2021 ◽  
Author(s):  
James Nyirenda ◽  
Gershom B. Lwanga ◽  
Kaampwe M. Muzandu ◽  
David K. Chuba ◽  
Gibson M. Sijumbila
Keyword(s):  
Plant Extract ◽  
Hematological Parameters ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Root Extract ◽  
Dependent Manner ◽  
Phytochemical Screening ◽  
Aqueous Root Extract

Abstract Ethnopharmacological relevanceAnemia is a very serious condition in Zambia. One of the plants that has been used traditionally is Phyllanthus muellerianus where different parts of shrub are used to treat a number of diseases in Zambian folklore medicine. Earlier studies have investigated medicinal properties of its aqueous root extracts. This study evaluated the effect of P. muellerianus roots on the hematological indices of albino rats and determined its phytochemical profile. Aim of the studyTo carry out phytochemical screening of the root extract and assess the ant-anemic effect of the aqueous extract on laboratory rats with tail-bled induced anemia Materials and MethodsThirty-six male albino rats placed in six groups were used for the study. The groups comprised the 100, 200, and 400 mg/kg plant extract, Ranferon (200 mg/kg) positive control, anemic non treated control and a normal (non-anemic) control. Anemia, induced through bleeding of the rats, was defined as hemoglobin (Hb) levels less than 12 g/dL. The anti-anemic potential of the plant was determined by comparing its effect on the hematological parameters of rats on treatment to that of the control group.ResultsAfter treatment, rats on the 400 mg/kg plant extract dose showed the greatest increase in the mean values for Hb, Packed cell volume (PCV) and RBC count were 43.3±1.2%, 15.4±0.3 g/dL and 6.3±0.3 x106 /mL respectively, when compared to the negative control group (P < 0.05). Phytochemical screening revealed positive results for alkaloids, flavonoids, saponins, glycosides, steroids, triterpenoids and tannins with varying amounts.Conclusions. The aqueous root extract of P. muellerianus was efficacious against anemia in a dose-dependent manner. The phytochemical compositions seem to be responsible for its hematopoietic properties. Thus, the root decoction of P. muellerianus is useful in alleviating anemia and the results lend credence to its use in traditional medicine in the management of anemia.

scholarly journals Functional and Structural Effects of Erythropoietin Subconjunctival Administration in Glaucomatous Animals

2018 ◽  
Vol 3 (2) ◽  
pp. 1-11 ◽  
Author(s):  
Ana Paula Resende ◽  
Serge G. Rosolen ◽  
Telmo Nunes ◽  
Berta São Braz ◽  
Esmeralda Delgado
Keyword(s):  
Neuroprotective Effect ◽  
Treated Group ◽  
Retinal Function ◽  
Function Evaluation ◽  
Control Group ◽  
Albino Rats ◽  
Subconjunctival Injection ◽  
Beneficial Effects ◽  
Structural Evaluation ◽  
Retinal Structure

Purpose: The present study aimed to assess functional and structural benefits of erythropoietin (EPO) when administered subconjunctivally in the retina of glaucomatous rats using electroretinography (ERG) and retinal thickness (RT) measurements. Methods: Glaucoma was experimentally induced in 26 Wistar Hannover albino rats. Animals were divided into 2 groups of 13 animals each: a treated group receiving a unique subconjunctival injection of 1,000 IU of EPO and a control group receiving a saline solution. In each group, 7 animals were used for retinal function evaluation (ERG) and 6 animals were used for retinal structural evaluation (histology). RT was measured, dorsally and ventrally, at 500 μm (RT1) and at 1,500 μm (RT2) from the optic nerve. Results: Retinal function evaluation: for both scotopic and photopic conditions, ERG wave amplitudes increased in the treated group. This increase was statistically significant (p < 0.05) in photopic conditions. Structural evaluation: for both locations RT1 and RT2, the retinas were significantly (p < 0.05) thicker in the treated group. Conclusion: Subconjunctival EPO administration showed beneficial effects both on retinal structure and on retinal function in induced glaucoma in albino rats. This neuroprotective effect should be applied in other animal species.

Sign in / Sign up

Export Citation Format

Share Document