scholarly journals THE EFFECT OF GAMMA IRRADIATION TO THE PHENOTYPIC OF TWO AGLONEMA VARIETIES

2017 ◽  
Vol 2 (2) ◽  
pp. 21-26
Author(s):  
Arya Widura Ritonga ◽  
Dewi Sukma
Keyword(s):  
Gamma Ray ◽  
Phenotypic Diversity ◽  
Economic Value ◽  
Leaf Length ◽  
Leaf Color ◽  
Group Design ◽  
Irradiation Treatment ◽  
Two Factors ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves

Increased phenotypic diversity is needed to increase the economic value of Aglaonema. However, information on increasing phenotypic diversity of Aglaonema using gamma-ray irradiation has not been widely known. This study aimed to investigate the effect of gamma ray irradiation treatment to the performances of two varieties of Aglaonema. This research was arranged factorially using randomized group design (RKLT) of two factors consisting of 8 combinations of treatments that are 4 level of irradiation dose and 2 Aglaonema varieties. The results showed that the induction of gamma ray irradiation decreased the % viable of the plants, the number of leaves, leaf length, leaf width, and the % green color as well as increased the % blue on the leaves of Aglaonema Butterfly and Aglaonema Siam Aurora. The interaction between dose of irradiation and aglaonema varieties was obtained in the % red of leaf color. Both of Aglaonema varieties had a high radiosensitivity with LD50 values ranged of 16.70 - 17.14 Gy

scholarly journals Keragaan Anggrek Persilangan ♀ Vanda Celebica X ♂ Vanda Dearei Hasil Iradiasi Sinar Gamma

2017 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Sri Hartati ◽  
Ahmad Yunus ◽  
Fajar Nugroho
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Color Change ◽  
Leaf Length ◽  
Leaf Color ◽  
Color Changes ◽  
Dose Irradiation ◽  
Randomized Design ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation

<p>Variation on orchid is an advantage that allows plant as breeding material. Orchid in plant breeding has a target to increase plant genetic diversity character who favored by the consumer. Efforts to increase genetic diversity among others by artificial mutation using gamma ray irradiation. This research aims to study the influence of gamma ray irradiation dose against the performance of morphology and growth of crossbred orchids ♀Vanda celebica x ♂Vanda dearei. Research using completely randomized design single factor that is dose gamma ray irradiation 0 Gray, 10 Gray, 20 Gray, 30 Gray, 40 Gray and 50 Gray. Data were analyzed descriptively, compared plant irradiation treatments with control plants to determine the effect of irradiation on morphology and growth plant. The results showed dose irradiation treatment increased the diversity of plant height, leaf amount, leaf length, leaf color changes, the amount and length of roots. 10 Gray dose irradiation increases as height and length plant leave. 30 Gray dose irradiation increase in the number of leaves. Leaf color change occurs at 20 irradiation treatments Gray and 50 Gray.</p>

scholarly journals Improving Performance of Coleus through Mutation Induction by Gamma Ray Irradiation

2015 ◽  
Vol 2 (1) ◽  
pp. 26-32
Author(s):  
Syarifah Iis Aisyah ◽  
Yodi Marthin ◽  
M. Rizal M. Damanik
Keyword(s):  
Gamma Irradiation ◽  
Dose Level ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Mutation Induction ◽  
Coleus Blumei ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves ◽  
Short Time

The objective of this study is to study the effect of gamma ray irradiation (15, 30, 45, and 60 gray (Gy) to determine Lethal Dose 50 (LD50) values, and to obtain new Coleus variances in a relatively short time. The study was conducted in a greenhouse at Cikabayan experimental field, Bogor Agricultural University, Darmaga, Bogor in May to July 2013. Gamma irradiation treatment significantly affected height, number of leaves, and number of nodes. Increasing the dose level of gamma irradiation tend to inhibit plant growth. LD50 for yellow/green, green/brown, variegated green/brown of Coleus blumei, and Coleus amboinicus Lour were 48.66, 65.2, 52.81, and 37.62 Gy respectively. C. amboinicus  irradiated at a dose level of 45 Gy had different leaf shapes compared to control. Keywords:  Coleus, gamma ray irradiation, LD50 values, ornamental plant, torbangun

scholarly journals Induksi Mutasi Kara Benguk (Mucuna pruriens L.) Menggunakan Iradiasi Sinar Gamma

2020 ◽  
Vol 22 (2) ◽  
pp. 105
Author(s):  
Siti Hartati Yusida Saragih ◽  
Khairul Rizal ◽  
Kamsia Dorliana Sitanggang
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Mucuna Pruriens ◽  
Mutation Induction ◽  
High Genetic Diversity ◽  
Group Design ◽  
Planting Material ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves

<p>Breeding kara benguk with mutation induction techniques using gamma ray radiation is expected to produce genetic diversity. The purpose of the study is to determine the value of Lethal Dose 50 (LD50) and obtain the genetic diversity of the kara benguk plant. The research was conducted at PAIR BATAN and Agrotechnology experiment, Universitas Labuhanbatu from May-July 2020. The planting material used was kara benguk seeds. The method used was Randomized Complete Group Design (RCGD) with one factor which was dose of irradiation with six levels (0, 200, 250, 300, 350 and 400 Gy) and three replications. Each replication consisted of 20 plants, so there are a total of 360 experimental plants. The results showed that the value of LD50 kara benguk in 3 weeks after planting was 281,472 Gy. Gamma ray irradiation increases the high genetic diversity of plants and the number of leaves at a dose of 350 Gy.</p>

scholarly journals Effects of Gamma Irradiation on Phenotypic Changes in Vanda Hybrid

2021 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Sri Hartati ◽  
Prasetyo Prasetyo ◽  
Endang Setia Muliawati
Keyword(s):  
Gamma Irradiation ◽  
Plant Height ◽  
Gamma Ray ◽  
Ornamental Plants ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Leaf Length ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves ◽  
Light Green

<p>Vanda Orchid is one of the most popular ornamental plants. Orchids have slow growth. One way to overcome this weakness is by using gamma-ray irradiation. This research aimed to study the morphological characters of various radiation doses resulting from the crossing of the Vanda Orchid. This study was carried out by observing the characters of vegetative growth for each plant on 0 Gy, 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy doses of gamma irradiation treatments. The observed variables were the increase in plant height, leaf length, root length, number of roots, number of leaves, leaf width, and leaf color. The results showed that the dose of gamma-ray irradiation were able to increase plant height. 10 Gy and 20 Gy, gamma-ray irradiation doses, resulted in discolored yellowing of orchid leaves. The discoloration of orchid leaves to light green occurs at irradiation doses of 30 Gy, 40 Gy, and 50 Gy. The morphological characteristics of <em>Vanda</em> sp. hybrid results gamma irradiation are high at dose 10-40 Gy.</p><p> </p><p>Keywords:<strong> </strong>characteristics; morphological; orchid.</p>

scholarly journals KOMBINASI MEDIA TANAM DAN PEMBERIAN BERBAGAI DOSIS PUPUK GROW QUICK LB TERHADAP PERTUMBUHAN ANGGREK DENDROBIUM (Dendrobium sp.) PASCA AKLIMATISASI

1930 ◽  
Vol 8 (01) ◽  
pp. 91-97
Author(s):  
Nofripa Herlina ◽  
Novia Gesriantuti ◽  
Anis Restiawati
Keyword(s):  
Economic Value ◽  
Leaf Length ◽  
Leaf Width ◽  
Growing Media ◽  
Randomized Design ◽  
Two Factors ◽  
The Media ◽  
Number Of Leaves ◽  
Medium Type

Dendrobium orchid is one of the very popular ornamental plant because of the flower is beautiful and has a high economic value. An effort to prevent the orchid from extinetion is by means of in vitro propagation. The study aims to look at the effect of various doses of fertilizers Grow Quick LB and types of growing media and their interaction on the growth of Dendrobium sp. post acclimatization.The completely randomized design was used with two factors. The firs factor was type medium (charcoal+fern, charcoal+cocopeat), the second factor was type of Grow Quick LB fertilizer (0ml/L, 1ml/L, 2ml/L, 3ml/L). Parameters observed were, plantlet height, leaf length, leaf width, and number of leaves. The results showed that the dosing of Grow Quick LB fertilizer 1ml/L showed the highest values for the parameters width of leaves and number of leaves, whereas a dose of 2ml/L showed the highest values for the parameters plantlet height, leaf length. A combination of types of growing media charcoal+cocopeat showed a better effect than the media charcoal+fern on all parameters. The interaction of medium type and Grow Quick LB fertilizer significantly different with plantlet height and leaf length and not significantly different with parameters leaf width, and number of leaves.

scholarly journals Gamma Irradiation Treatment of Bactrocera dorsalis Hendel (Diptera: Tephritidae) in Snake Fruit

2019 ◽  
Vol 23 (2) ◽  
pp. 242
Author(s):  
Novi Kusumaning Astuti ◽  
Suputa Suputa ◽  
Nugroho Susetyo Putra ◽  
Murni Indarwatmi
Keyword(s):  
Gamma Ray ◽  
Fruit Fly ◽  
Bactrocera Dorsalis ◽  
The Third ◽  
Minimum Dose ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation

Snake fruit (Salacca zalacca) is a unique fruit and it has decadent prospects to be developed as an export commodity. Nevertheless, oriental fruit fly, Bactrocera dorsalis Hendel seems to decrease the quantity and quality of this fruit. On the other side, irradiation has been developed as a standard quarantine treatment to disinfest fruit fly on fruit. The objective of this research was to determine impact of irradiation by Cobalt-60 gamma-ray on the development and survivorship of eggs and the third instar of fruitfly larvae using in-vitro and in-vivo approaches and minimum dose of Cobalt-60 gamma rays applied for snake fruit. Six doses of gamma-ray, i.e. 0 (control), 25, 50, 75, 100, 125 and 150 Gy were used in this experiment. The results showed that Cobalt-60 disturbed development and survivor rate of B. dorsalis. The development of eggs into pupa was failed when treated with Cobalt-60 at any doses, while the third instar larvae failed to become adult when irradiated with 75, 100 and 150 Gy of gamma-ray. The impacts were increased with the increment of dose. Furthermore, impact of gamma-ray irradiation was greater on eggs compared to the third instar of larvae. The minimum dose of irradiation to prevent adults emerge was 118 Gy.

scholarly journals Keragaman warna bunga m1 tanaman aster (Callistephus chinensis) Hasil induksi mutasi iradiasi sinar gamma

2018 ◽  
Vol 2 (1) ◽  
pp. 52
Author(s):  
Yanuar Rizqiani ◽  
Florentina Kusmiyati ◽  
Syaiful Anwar
Keyword(s):  
Research Design ◽  
Gamma Ray ◽  
Flower Colour ◽  
Purple Colour ◽  
Randomized Design ◽  
Irradiation Treatment ◽  
Flower Diameter ◽  
Gamma Ray Irradiation ◽  
Completely Randomized Design ◽  
Stalk Length

The aims of research was to determine the effect of gamma ray on the flower colour of Daisies. The research design was completely randomized design with five replicates. The irradiation treatment of gamma ray were 0 Gy, 5 Gy, 10 Gy, 15 Gy, 20 Gy. Parameters observed were stalk length, time of flowering, number of flowers, flower diameter, and flower colour. The collected data were analyzed by analysis of variance (ANOVA) and continued by Least Significance Different (LSD) of 5% level. The result showed that irradiation of gamma ray did not affected stalk length, number of flowers, and time of flowering. The gamma ray irradiation had a significant effect on flower diameter. Gamma ray irradiation significantly decreased the diameter of flower. The flower colour at doses 0 Gy (control) was purple. Colour flower was varied from dark purple to pink at irradiation 10 Gy and 15 Gy. Keywords: Callistephus chinensis, mutation, irradiation, colour of flower.

scholarly journals THE ADAPTATION LEVEL OF ALFALFA (MEDICAGO SATIVA L.) THAT IRRADIATED WITH GAMMA RAYS ON A FIELD SCALE

Pastura ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Prihantoro, I. ◽  
Anandia, A. ◽  
Aryanto, A. T. ◽  
Setiana, M. A. ◽  
Karti, P. D. M. H.
Keyword(s):  
Medicago Sativa ◽  
Gamma Rays ◽  
Gamma Ray ◽  
Adaptation Level ◽  
Field Scale ◽  
Leaf Color ◽  
Randomized Design ◽  
Gamma Ray Irradiation ◽  
Medicago Sativa L ◽  
Dark Green

Alfalfa (Medicago sativa L.) a high nutritious and palatability legume for ruminant. Constrain of alfalfa availability in Indonesia are due to the plant adaptability in tropical environment. Aim of the study was to measure the adaptation level of alfalfa that irradiated with gamma rays on a field scale. The study consisted of four types of plant sources, mutation plants with different gamma ray levels (0Gy, 200Gy, 300Gy and 400Gy). Plant tillers was analyzed with a complete randomized design with 3 replications, 20 plants per replication. Growth capability, leaf color, flowering time and pest attack level were analyzed descriptively. The results showed that alfalfa irradiated with 300 Gy significantly (p<0.05) produced highest tillers. Irradiated 300 Gy plant gave better result on growth capability, flowering capability, and number of not attack plant from pests. The higher level of gamma ray irradiation showed the changed in leaf color levels from dark green to light green. Keywords: alfalfa (Medicago sativa L.), mutation, gamma ray irradiation, field scale

scholarly journals Regenerasi Tanaman dari Kalus Tebu yang Diiradiasi Sinar Gamma pada Medium dengan PEG 6000

2017 ◽  
Vol 44 (3) ◽  
pp. 322
Author(s):  
Agus Purwito ◽  
Nidya Ravenska ◽  
Dan Awang Maharijaya
Keyword(s):  
Drought Stress ◽  
In Vitro Selection ◽  
Gamma Ray ◽  
Peg 6000 ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Two Factors ◽  
Selection Medium ◽  
Gamma Ray Irradiation

ABSTRACT<br /><br />Sugarcane cultivars tolerant to drought stress are needed for specific location. In vitro selection can be used to obtain plants tolerant to drought stress through regeneration of callus in the culture medium. The purpose of this study was to obtain regenerants from irradiated callus on the stress medium. The experimental design was a completely randomized design with two factors. The first factor was the regeneration medium (RG) added with PEG 6000, i.e., 0%, 5%, 10% and 15%, while the second factor was the dose of gamma ray irradiation i.e. 0 krad, 5 and 10 krad, and 15 krad. There were 12 treatments, each treatment was repeated 18 times and each repetition was a culture bottles planted with three clums of callus 10 mm diameter. RG medium was the MS medium added with 0.5 mg L-1 BAP, Kinetin 0.1 mg L-1 and IBA 1.0 mg L-1. The higher the concentration of PEG, the less regenerants were produced. Regenerants could be generated from the selection medium PEG up to 15%. Several shoots still produced from callus irradiated with 10 krad. Selection medium with PEG up to 10% decreased the precentage of albino regenerants. The gamma ray irradiation dose and the concentration of PEG significanly affected the number of  roots produced.<br /><br />Keywords: drought tolerant, in vitro, polyethylene glycol, plantlets, albino<br /><br />

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