Antagonistic activity of marine bacterial isolates on common human pathogenic bacteria
Antagonistic activity of marine bacteria against common human pathogens was studied in this project. Marine animal surface bacterial flora was isolated from selected animal collected from local fishermen. The sample bacteria were isolated by serial dilution followed by spread plate method using marine agar (ZMA). About 11 bacterial isolates were selected and screened for antimicrobial activity through agar well diffusion assay. Among 11 isolates, 7 isolates exerted an inhibitory effect against human pathogenic organisms (E.coli, S.aureus, Klebsiella and Candida sp.). The isolates were then investigated for antibiotic production by cross species signal transfer mediated induction, 2 isolates (Sh-2 and PJR-3) exhibited enhanced activity against human pathogenic organisms (E.coli, S.aureus and Candida sp.). These 2 isolates were characterized phenotypically by morphological techniques (which revealed the strains as gram-positive and gram negative respectively) and physiologically by conventional tests.