scholarly journals Comparative evaluation of hydrolysates as a basis for the construction of a nutrient medium for the cultivation of Listeria monocytogenes

2021 ◽  
Author(s):  
N. M. Khaptanova ◽  
А. S. Ostyak ◽  
S. V. Lukyanova ◽  
V. I. Kuznetsov ◽  
N. M. Аndreevskaya ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Enzymatic Hydrolysis ◽  
Nutrient Medium ◽  
Comparative Evaluation ◽  
Raw Materials ◽  
Amino Nitrogen ◽  
Test Strain ◽  
Nutrient Media ◽  
Microbiological Methods ◽  
The Common

The objective is to perform a comparative evaluation of the pancreatic hydrolysates prepared from fish and squid to determine the optimal culture medium for Listeria monocytogenes.Materials and methods. The following raw materials were used in the study: Pacific Herring (Clupea pallasii), Alaska Pollock (Gadus chalcogrammus), Common Roach (Rutilus rutilus lacustris), European Squid (Loligo vulgaris). The raw materials were subjected to enzymatic hydrolysis using the pancreas (according to Hottinger). A study of the physicochemical properties of pancreatic hydrolysates (content of free amino nitrogen (FAN), acidity of fish hydrolysates, the amino acid composition) was carried out.. The specific activity of nutrient media during the cultivation of the test strain L. monocytogenes 766 was assessed by a complex of microbiological methods.Results and discussion. The highest content of FAN at the end of enzymatic hydrolysis was observed in the pancreatic hydrolysate of the common roach (6%), the acidity of the hydrolysate remained stable from 6th to 13th day of the hydrolysis process (pH 7.2). Pancreatic hydrolysate of the common roach contained a number of amino acids that are most essential for the growth of Listeria. An assessment of the biological properties of nutrient media prepared on the basis of the obtained hydrolysates demonstrated that the best results in terms of sensitivity and germination of L. monocytogenes 766 showed a nutrient medium based on the pancreatic hydrolysate of the common roach. During the cultivation of L. monocytogenes 766 the test strain retained its morphological and cultural properties and did not show signs of dissociation.Conclusion. The research results have shown that the pancreatic hydrolysate of the common roach is a promising protein basis for the construction of an experimental environment for listeria. 

scholarly journals Designing a Nutrient Medium for the Accumulation of Microbial Mass of Listeria

2020 ◽  
Vol 5 (4) ◽  
pp. 60-66
Author(s):  
N. M. Khaptanova ◽  
S. V. Lukyanova ◽  
V. I. Kuznetsov ◽  
N. G. Gefan ◽  
N. M. Аndreevskaya ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Culture Medium ◽  
Specific Activity ◽  
Germination Rate ◽  
Biochemical Properties ◽  
Quantitative Composition ◽  
Nutrient Media ◽  
Microbiological Methods ◽  
Microbial Mass

Background. To obtain reliable results of laboratory studies on the identification of Listeria, the presence of certified diagnostic agglutinating Listeria sera is required. An important step in the manufacturing process of such medical devices for in vitro diagnostics requires effective nutrient media for the accumulation of listeriosis microbe. Aim of the research. To develop an effective nutrient medium for the accumulation of bacterial mass of Listeria. Materials and methods. The object of the study was an experimental culture medium for Listeria cultivation. As a control, we used nutrient agar for the cultivation of microorganisms (fish meal hydrolysate, FMH-agar) and meat-peptone agar with 1 % glucose (MPA with 1 % glucose). The specific activity of nutrient media during cultivation of the test strain Listeria monocytogenes 766 was evaluated using a complex of microbiological methods. Results. The optimal base of the nutrient medium for Listeria cultivation has been selected: pancreatic hydrolysate of river magpie fish (Rutilus rutilus lacustris) and hydrolysate of meat water production waste. The qualitative and quantitative composition of the nutrient medium has been developed, its physical, chemical and biological properties have been studied. It was found that after 24 hours of incubation at 37 °C, the nutrient medium provided the growth of typical Listeria colonies. The germination rate was 85 %, which is higher compared to the growth of the culture on MPA with 1 % glucose and GRM agar by an average of 21 % (p < 0,05). Conclusion. The experimental culture medium for Listeria cultivation provided growth of colonies of the test strain L. monocytogenes 766 with the preservation of characteristic cultural, morphological and biochemical properties, and, in terms of germination and growth rate, exceeded the control media. The developed nutrient medium provides effective growth of Listeria and can be used as a medium for the accumulation of microbial mass.

scholarly journals Characteristics growth of mycelium of mushroom shiitake Lentinula Edodes (Berk.) Pegler in vitro

2019 ◽  
pp. 20-25
Author(s):  
T. Ivanova ◽  
N. Voloschuk
Keyword(s):  
Growth Rate ◽  
Light Microscopy ◽  
Pure Culture ◽  
Nutrient Medium ◽  
Sodium Selenite ◽  
Lentinula Edodes ◽  
Ph Value ◽  
Nutrient Media ◽  
Microbiological Methods

Goal. The purpose of this work is to study the growth of the mycelium of the fungus Lentinula Edodes (Berk.) Pegler on nutrient media of different composition and also to study the features of the use of Avatar-1 micro fertilizer and sodium selenite. Methods. Biotechnological research methods. Sodium selenite (Na2S eO3) at a concentration of 1.0 mmol / l was also used. Pure culture of the mushrooms had a very dense structure and white color. In our work, we used biotechnological methods — obtain this by subculturing the L. edodes strain in vitro; microbiological methods — obtaining pure culture of the fungus, the study of the cultural properties of the colonies. We set the pH value (pH) of nutrient media at the beginning and at the end of incubation. We applied mycological methods to measure the speed, density of growth and dry mass of mycelium. we used the light microscopy method. We performed statistical data processing.Method of light microscopy. Results. The experiments showed about acceleration of mycelial growth, mass and the greatest yield of mycelium L. edodes were on a nutrient medium with microfertilizer Avatar-1. In the experiment, it was found that the maximum overgrowth of the medium by mycelium occurs at 7 days. We have been proved that in the «Avatar-1» nutrient medium there was an increase and consolidation of bifurcated hyphae and buckles of L. edodes. There was even germination of mycelium that did not have too thin or thick hyphae. The dependence of growth rate on the type of nutrient medium. Also, the of doses of the drug, which effectively influences the technology of obtaining primary mycelium L. edodes. Conclusions. The dependence of the growth rate on the type of nutrient medium, the dose administration rate, as well as the cultivation regimes, that effectively influence and cheapen the technology of obtaining the primary mycelium L. edodes is demonstrated.

scholarly journals Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus

2020 ◽  
Vol 5 (1) ◽  
pp. 47-53
Author(s):  
S. V. Lukyanova ◽  
N. G. Gefan ◽  
S. N. Adamovich ◽  
E. N. Oborina ◽  
N. M. Khaptanova ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Rate ◽  
Nutrient Medium ◽  
Biological Properties ◽  
Test Strain ◽  
Biologically Active ◽  
Nutrient Media ◽  
Growth Stimulator ◽  
Biologically Active Compound ◽  
Meat Peptone Agar

Background. Development of nutrient media ensuring the maximum growth rate of pathogens of dangerous infectious diseases while preserving their biological properties is extremely important. A promising direction in this area seems to be the use of synthetic microbial growth biostimulants.The aim of the work is to study the possibility of improving nutrient media for the cultivation of Listeria and Staphylococcus using a biologically active compound tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanylacetate.Materials and methods. The object of the study was experimental nutrient medium for the cultivation of Listeria used for the culturing of the test strain Listeria monocytogenes 766. As a comparison medium, commercial medium Fraser broth to which agar was added at a concentration of 1.5 %, was used. The test strain Staphylococcus aureus ATCC 6538-P (FDA 209-P) was cultivated on meat-peptone agar with 1% glucose. The compound tris(2-hydroxyethyl) ammonium (4-chlorophenyl)sulfanylacetate at a concentration of 10–4 wt. % was studied as a growth stimulator. A nutrient medium without a stimulant served as a control. The specific activity of nutrient media (germination rate, medium sensitivity, growth rate and stability of the main biological properties of microorganisms) was evaluated by the microbiological method.Results. Studies have shown that the addition of a growth stimulator to nutrient media contributes to the growth of colonies (by 10–50 %) and a decrease in the time of their development. When growth stimulator was added to the nutrient medium for the cultivation of Listeria, the initial growth of colonies of the L. monocytogenes 766 test strain after 12 hours of cultivation and growth of colonies of the test strain S. aureus ATCC 6538-P after 6 hours of cultivation on the meat-peptone agar with 1% glucose was observed.Conclusion. Thus, the addition of a growth biostimulator tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanyl acetate at a concentration of 10–4 wt. % in the nutrient medium accelerates the growth of Listeria and Staphylococcus, allows to reduce the time of issuance of the analysis result in half.

On the use of the main nutrient medium from cottage cheese for growing microbes

1937 ◽  
Vol 33 (3) ◽  
pp. 342-345
Author(s):  
O. V. Geltzer
Keyword(s):  
Soviet Union ◽  
Nutrient Medium ◽  
Pathogenic Bacteria ◽  
Raw Materials ◽  
Cottage Cheese ◽  
Medical Institute ◽  
Nutrient Media ◽  
Pigment Formation ◽  
Complete Replacement ◽  
The Soviet Union

The main condition for the suitability of a nutrient medium for growing microbes is the presence of protein substances in it. Research thought so far has been in the direction of replacing animal protein with vegetable protein. We see this in a large number of German recipes, the so-called. Ersaznhrbden (surrogate environments). In order to find the most complete replacement of animal proteins in bacteriological nutrient media, the composition of many nutrients was determined in detail. Soy proteins turned out to be the closest in composition to animal proteins. Since soybeans are not widespread in all regions of the Soviet Union, Russian researchers had to look for other sources of raw materials, more accessible than soybeans. The Scientific Medical Institute proposed nutrient media from peas. But, apparently, these media are not always suitable (Calc), if only in view of the limited number of generations obtained for the p-p of some pathogenic bacteria (bang. Shiga dysentery), the absence of pigment formation (miraculous rod).

scholarly journals Development of a Digital Twin for Enzymatic Hydrolysis Processes

Processes ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1734
Author(s):  
Christian Appl ◽  
Frank Baganz ◽  
Volker C. Hass
Keyword(s):  
Enzymatic Hydrolysis ◽  
Raw Materials ◽  
Starch Hydrolysis ◽  
Strategy Development ◽  
Enzymatic Process ◽  
Nutrient Media ◽  
Renewable Raw Materials ◽  
Digital Twins ◽  
Hydrolysis Process ◽  
Kinetics Of

Enzymatic hydrolysis processes can be used to produce organic nutrient media from renewable raw materials. However, many of these processes are not optimally designed, so expensive enzymes and substrates are wasted. Mathematical models and Digital Twins (DTs) are powerful tools, which can be used to optimize bioprocesses and, thus, increase the yield of the desired products. Individual enzymatic hydrolysis processes have already been modeled, but models for the combined starch hydrolysis and proteolysis, or DTs, are not available yet. Therefore, an easily adaptable, dynamic, and mechanistic mathematical model representing the kinetics of the enzymatic hydrolysis process of the combined starch hydrolysis and proteolysis was developed and parameterized using experimental data. The model can simulate the starch hydrolysis process with an agreement of over 90% and the proteolysis process with an agreement of over 85%. Subsequently, this model was implemented into an existing DT of a 20 L stirred tank reactor (STR). Since the DT cannot only map the kinetics of the enzymatic process, but also the STR with the associated periphery (pumps, heating jacket, etc.), it is ideally suited for future process control strategy development and thus for the optimization of enzymatic hydrolysis processes.

scholarly journals Improvement of Enzymatic Saccharification of Cellulose-Containing Raw Materials Using Aspergillus niger

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1360
Author(s):  
Ekaterina Budenkova ◽  
Stanislav Sukhikh ◽  
Svetlana Ivanova ◽  
Olga Babich ◽  
Vyacheslav Dolganyuk ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Filter Paper ◽  
Raw Materials ◽  
Enzymatic Saccharification ◽  
Wood Chip ◽  
Cellulase Activity ◽  
Wood Chips ◽  
Acid Pretreatment ◽  
Hydrolysis Of

Enzymatic hydrolysis of cellulose-containing raw materials, using Aspergillus niger, were studied. Filter paper, secondary cellulose-containing or starch-containing raw materials, miscanthus cellulose after alkaline or acid pretreatment, and wood chip cellulose, were used as substrates. The study focused on a wild A. niger strain, treated, or not (control), by ultraviolet (UV) irradiations for 45, 60, or 120 min (UV45, UV60, or UV120), or by UV irradiation for 120 min followed by a chemical treatment with NaN3 + ItBr for 30 min or 80 min (UV120 + CH30 or UV120 + CH80). A mixture of all the A. niger strains (MIX) was also tested. A citrate buffer, at 50 mM, wasthe most suitable for enzymatic hydrolysis. As the UV exposure time increased to 2 h, the cellulase activity of the surviving culturewas increased (r = 0.706; p < 0.05). The enzymatic activities of the obtained strains, towards miscanthus cellulose, wood chips, and filter paper, were inferior to those obtained with commercial enzymes (8.6 versus 9.1 IU), in some cases. Under stationary hydrolysis at 37 °C, pH = 4.7, the enzymatic activity of A. niger UV120 + CH30 was 24.9 IU. The enzymatic hydrolysis of secondary raw materials, using treated A. niger strains, was themost effective at 37 °C. Similarly, the most effective treatment of miscanthus cellulose and wood chips occurred at 50 °C. The maximum conversion of cellulose to glucose was observed using miscanthus cellulose (with alkaline pretreatment), and the minimum conversion was observed when using wood chips. The greatest value of cellulase activity was evidenced in the starch-containing raw materials, indicating that A. niger can ferment not only through cellulase activity, but also via an amylolytic one.

scholarly journals THE PRODUCTION OF SELECTIVE ADDITIVES TO GROWING MEDIUM DRIGALSKI LACTOSE AGAR

2019 ◽  
pp. 95-102
Author(s):  
Владимир Ермаков ◽  
Vlyadimir Ermakov ◽  
Оксана Датченко ◽  
Oksana Datchenko ◽  
Юлия Курлыкова ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
First Generation ◽  
Animal Species ◽  
Agar Medium ◽  
Nutrient Media ◽  
Gram Negative ◽  
Selective Media ◽  
Growing Medium ◽  
Agar Media ◽  
Growth And Reproduction

The purpose of the study is to improve the selective supplement for selective media with the purpose to produce enterobacteria. Tasks of the study are to identify the sensitivity of strains obtained of enterobacteria in regard to an-tibiotics; develop a new selective supplement with antibiotics to the nutrient medium Drigalski Lactose Agar. Media should have a content that in the best way possible ensures the growth and reproduction of microorganisms of cer-tain species or family. Intensive biotechnology development and Microbiology allows today to develop new nutrient media and modify the already existing content of media. The object of the study was a new selective additive with antibiotics to the nutrient medium Drigalski Lactose Agar. 253 isolates of bacteria produced from the intestinal mi-crobiotope of different animal species have been the Material for research. The study was conducted in the period from 2010 to 2017. Carbenicillin 30±2.3 from the group of carboxypenicillins and piperacillin 37±2.5 from the group of ureidopenicillins, kanamycin 24±1.5, amikacin 26±1.7 and gentamicin 25±0,8, cefepime 38±3.2 from the group of IV generation cephalosporins, tetracycline 28±1.6, doxycycline 34±2.3 and chloramphenicol 31±2.5, nalidixic acid 37±2.8, trimethoprim 35±3,4 demonstrated the greatest antimicrobial activity against all cultures of enterobac-teria that has been achieved. The high resistance of enterobacteria was shown to benzylpenicillin from the group of natural penicillins, to streptomycin, cephalotine from the group of cephalosporins of the first generation, to polymyx-in B, to ofloxacin (tarivid) and metronidazole. Antibacterial drugs effective against the accompanying gram-positive and gram-negative microflora were considered as the samples of the selective components. Vancomycin from the group of glycopeptides, linezolid from the group of oxazolidinones, and telithromycin from the group of ketolides were chosen. Antibiotics vancomycin and telithromycin in a dose of 0.008 g/dm3, linezolid 0.004 g/dm3 were cho-sen as the selective additive to Drigalski Lactose Agar medium.

scholarly journals FLAVONOIDS OF IRIS SIBIRICA L. GROWN IN VITRO CULTURE

2021 ◽  
pp. 301-308
Author(s):  
Olesya Nikolayevna Mazko ◽  
Lyudmila Ivanovna Tikhomirova ◽  
Lyudmila Vladimirovna Shcherbakova ◽  
Natal'ya Grigor'yevna Bazarnova ◽  
Dmitriy Alekseyevich Karpitsky
Keyword(s):  
In Vitro Culture ◽  
High Performance ◽  
Raw Materials ◽  
Quantitative Composition ◽  
Qualitative Composition ◽  
Nutrient Media ◽  
Qualitative And Quantitative ◽  
Clonal Micropropagation ◽  
Intact Plants

The aim of this study was to evaluate the effect of 6-benzylaminopurine (BAP) separately and in interaction with auxins on the change in the qualitative and quantitative composition of flavonoids in the raw materials of regenerating plants Iris sibirica L. Cambridge grade in comparison with aeroponic and intact raw materials using the method of high-performance liquid chromatography. Raw materials of I. sibirica Cambridge variety obtained in vitro culture had a richer qualitative composition of flavonoids than intact plants. The dependence of the accumulation of flavonoids on the concentration of 6-benzylaminopurine in nutrient media was noted. The presence of 13 compounds was observed in extracts of 70% ethyl alcohol from regenerating plants grown at the lowest concentration of BAP (1.0 µM) within the experiment. In quantitative terms, the flavonoid apigenin was maximally determined on a medium with BAP 1 µM, and kaempferol - on media with BAP 5.0 µM, supplemented with auxins. For a medium with 7.5 µM BAP, the lowest variety of compounds was observed (9) and the lowest kaempferol content. Auxins influenced the synthesis of flavonoids. The amount of flavonoids in all variants of the experiment increased by an average of 13% in the presence of auxins. The stages of the technological process of obtaining raw materials I. sibirica Cambridge variety on the basis of clonal micropropagation and cultivation in aeroponics conditions allowed to obtain raw materials that do not contain heavy and toxic metals, are not infected with pathogens and pests. With 1 m2 of useful area of aeroponics for 1 year, it is possible to collect 5 times more raw materials than with field cultivation. According to the qualitative composition of phenolic compounds, aeroponic raw materials are identical to intact plants.

scholarly journals Selection of Fish Raw Material Suppliers by Integrating ANP and Topsis Methods in CV. Riki Utama Mandiri

2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Aula Fajar Iman Sakti ◽  
Wiwik Sulistiyowati
Keyword(s):  
Supplier Selection ◽  
Raw Materials ◽  
Rank Order ◽  
Raw Material ◽  
Network Process ◽  
The Common ◽  
Common Problems ◽  
Frozen Fish ◽  
A Company ◽  
Selection Of

CV. Riki Utama Mandiri is a company in distributing an economic fish frozen product. This company distributed any kind of retail and wholesaler, both domestic and export. They distributing many frozen fish products variant such as Patin Fillet and Shark Fin. The all raw materials of those frozen seafood was obtained by three different suppliers. The common problems found in CV. Riki Utama Mandiri mostly about raw patin fish supplier which often committed delivery delays.  The purpose of this research is to fixing the supply chain management in deciding the more accurate selections of raw materials supplier. To overcome the common problems that happen. Analytical network process (ANP) will simplify the criteria weight values and sub criteria of each supplier. Meanwhile, technique for others reference by similarity to ideal solution (TOPSIS) method is used for giving a rank order of the alternative supplier. This research is expected for being a consideration for the company in obtaining a good and more effective kind of raw supplier. We also expecting the company for tighten supplier selection more effective way so that it can fullfilled the existing standard. Also to overcome the common problems such as delivery delays, competing raw materials with uncertain quality, and difficulty in sort out the raw materials due to size issues.

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