scholarly journals Analysis of βeta-carotene from Jongi (Dillenia Serrata Thunb.) as a source of vitamin A

2021 ◽  
Vol 10 (2) ◽  
pp. 184-190
Author(s):  
Khairun Nisa ◽  
Ryka Marina Walanda
Keyword(s):  
Chronic Disease ◽  
Vitamin A ◽  
Essential Role ◽  
Antioxidant Activities ◽  
Beta Carotene ◽  
Neurological Function ◽  
The Body ◽  
Yellow Orange ◽  
Β Carotene ◽  
Healthy Eyes

Beta-carotene is converted in the body to vitamin A, a powerful antioxidant that plays an essential role in maintaining healthy eyes, skin, and neurological function. Beta-carotene and other carotenoids have antioxidant activities and are valued for their ability to prevent chronic disease. β-carotene is found in yellow, orange, red, and green fruits. The purpose of this study was to determine the β-carotene in Jongi. Jongi (Dillenia Serrata Thunb.) is an endemic fruit of Sulawesi which contains β-carotene. The samples used in this study were raw jongi and ripe jongi extracted with n-hexane: acetone (1:4). To the samples were analyzed quantitatively using UV-Vis spectrophotometry at a wavelength of 450.00 nm. The results showed that the β-carotene in raw jongi was 0.3554 mg/100 g and the β- carotene in ripe jongi was 1.1841 mg/100 g. Ripe jongi consist of more β-carotene than the raw jongi, hence better as a source of Vitamin A.

scholarly journals Effectiveness of a Food-Based Intervention to Increase Breast Milk Vitamin A Content at One Month of Infancy

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 130-130
Author(s):  
Sumathi Swaminathan ◽  
Sumithra Selvam ◽  
Agnita Narendra ◽  
Tinku Thomas ◽  
Anil Vasudevan ◽  
...  
Keyword(s):  
Breast Milk ◽  
Vitamin A ◽  
Randomized Study ◽  
Beta Carotene ◽  
Maternal Blood ◽  
Energy Agency ◽  
Funding Sources ◽  
Deuterium Dilution ◽  
And Control ◽  
Β Carotene

Abstract Objectives Vitamin A requirements in early infancy are met only by breast milk intake. It is critical to ensure adequate breast milk vitamin A levels which also helps develop liver stores. The objective of the study was to evaluate the effect of a maternal food-based intervention on breast milk vitamin A content Methods Pregnant women (n = 50; 24 ± 1 week of gestation) were recruited for the randomized study. A 10 g of a green leafy vegetable powder (mint/coriander/curry) providing about 3200 μg β-carotene/day, for a period of 4 months up to 1 month of lactation were provided for intervention arm. Breast milk (BM) retinol concentration and BM volume were assessed. BM retinol and beta-carotene were assayed by HPLC and BM fat by creamatocrit method. BM retinol: fat ratio was calculated. The dose-to-mother deuterium dilution technique was used to estimate BM volume through enrichment of saliva measured by Fourier Transform Infrared Spectroscopy. Total BM retinol content was calculated from BM volume and the BM retinol (including beta-carotene:1 vitamin A RAE = 12 μg β-carotene). Inadequacy of intake was defined as proportion of infants with intake below a requirement of 400 μg RAE/day. Analysis of co-variance was performed after adjusting for age of mother, change in maternal blood retinol from baseline and BM fat, to compare outcomes between intervention and control arm. Results Maternal age, gestational age, socio-demographic characteristics and baseline vitamin A intake were comparable in both arms. Mean BM volume was similar in the 2 arms (676 ± 102 in intervention vs 630 ± 100 ml/day in control). BM retinol content (0.72 ± 0.12 vs 0.64 ± 0.11 μg/mL; P = 0.029) and BM retinol: fat ratio [0.41 (0.31, 0.47) vs 0.29 (0.21, 0.41), P = 0.011] were significantly higher in the intervention arm. The mean total BM retinol content was significantly higher in the intervention (482.2 ± 100.7 vs 406.5 ± 89.2 μg/day; P = 0.015; Cohen's effect size 0.80). Inadequacy of infant vitamin A intake was 14.3% in the intervention arm as against 39.1% in the control arm (P = 0.065). Conclusions The food-based intervention was effective in increasing vitamin A content in breast milk and thereby vitamin A intake in infants. Funding Sources International Atomic Energy Agency.

scholarly journals Diallel Analysis of Sweet Potato [Ipomoea batatas (L.) Lam] Genotypes for Combined Beta Carotene and Dry Matter Content in Southern Guinea Savanna, Nigeria

2019 ◽  
pp. 1-9
Author(s):  
M. S. Afolabi ◽  
O. B. Bello ◽  
G. O. Agbowuro ◽  
C. O. Aremu ◽  
M. O. Akoroda
Keyword(s):  
Vitamin A ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Dry Matter ◽  
Vitamin A Deficiency ◽  
Beta Carotene ◽  
Matter Content ◽  
Quality Trait ◽  
Dry Matter Content ◽  
Β Carotene

Roots of orange-fleshed sweet potato varieties currently available in Nigeria contain high quantities of β-carotene or pro-vitamin A but have high moisture content. These varieties have been found to be a cheap and crucially important remedy for vitamin A deficiency. The cream or white-fleshed varieties, on the other hand, have a sweet taste with high dry matter content, giving a dry texture, a quality trait preferred in Nigeria. Development of sweet potato genotypes that can combine these two important quality traits is the objective of this breeding work. A diallel experiment using six parental sweet potato genotypes crossed in all possible combinations were carried out and thirty progenies were evaluated for beta carotene (β-carotene) and dry matter content in Landmark University, Omu Aran, Kwara State, Nigeria. The 30 F1 progenies along with their parental lines were planted in the same field trial. The trial was laid out in 6 x 6 triple lattice in two replications. Highly significant (P≤ 0.01) differences were observed among the genotypes for the traits. The average β-carotene content among the progenies was 2.86 (mg/100g.f.w) while the dry matter cttgontent had a mean value of 31.89%. The cross progenies 199024.2 x Excel had the highest beta carotene (14.37mg/100g.f.w) content with the highest dry matter content (40.10%) and are therefore recommended for further evaluation.

scholarly journals Penentuan β-karoten dalam buah wortel (Daucus Carota) secara kromatografi cair kinerja tinggi (U-HPLC)

2019 ◽  
Vol 4 (1) ◽  
pp. 36
Author(s):  
Sonlimar Mangunsong ◽  
Rifqi Assiddiqy ◽  
Eka Puspa Sari ◽  
Priscila Natalia Marpaung ◽  
Rahma Arum Sari
Keyword(s):  
Free Radicals ◽  
Daucus Carota ◽  
High Performance ◽  
Calcium Salt ◽  
Beta Carotene ◽  
The Body ◽  
Organic Chemical ◽  
Chloroform Methanol ◽  
Β Carotene

The lifestyle of people who tend to be unhealthy causes a lot of free radicals in the body which can cause various diseases, especially degenerative diseases. To protect the body from attacks by free radicals, the body needs antioxidants such as β-carotene. One of the vegetables containing β-carotene is “wortel” Daucus carota, that  is very large in agriculture. The spread of beta carotene from carrots with chemical solvents has been done a lot, but without chemical solvents it has never been done. Based on the color, the content of β-carotene contained in carrots determines the content of beta carotene. The juice obtained by calcium salt was then centrifuged for 3000 rpm 15 minutes. The pellets are separated from the solution, evaporated to dryness, measured at a wavelength of 450-460. Pellets as beta-carotene calcium salts were analyzed by U-HPLC. The next pellet is measured at 460 wavelengths. The levels are determined by high performance liquid chromatography using C18 column and the mobile phase of chloroform-methanol (95:5) with a flow rate of 1 ml / minute at a wavelength of 460 nm. The content of β-carotene in the examination is up to 92,5%. The retention time obtained is 1,903 minutes. The results showed that this method can be used for the withdrawal and determination of β-carotene levels without organic chemical solvents.Pola  hidup masyarakat yang cenderung tidak sehat menyebabkan  banyak radikal bebas di dalam tubuh yang dapat mengakibatkan berbagai penyakit terutama penyakit degeneratif. Perlindungan tubuh dari serangan radikal bebas, perlu antioksidan seperti β-karoten. Salah satu sayuran  yang mengandung β-karoten adalah wortel, jumlahnya sangat banyak dalam pertanian. Berdasarkan warnanya, maka kandungan β-karoten yang terdapat dalam wortel menjadi penentu  kandungan beta karoten Metode ektraksi yang aman dari bahan pelarut kimia mutlak diperlukan.  Untuk mengetahui kandungan β-karoten dalam wortel,  lebih  dahulu dilakukan penghalusan  dengan blender, kemudian sari difilter /dipisahkan dari ampasnya. Sari  yangdiperleh ditambahkan garam kalsium kemudian disentrifus 3000 rpm  15 menit. Pelet dipisahkan dari larutannya, diuapkan hingga kering, Diukur pada panjang gelombang 450-460. Pelet sebagai garam kalsium betakaroten dianalisa dengan U-HPLC.  Pelet selanjutkan diukur pada panjang gelombang 460. ditetapkan kadarnya secara kromatografi cair kinerja tinggi menggunakan kolom C18 dan fase gerak kloroform–metanol (95:5) dengan laju alir 1 ml/menit pada panjang gelombang 460 nm. Kandungan β-karoten dalam pemeriksaan  sampai 92,5%. Waktu retensi yang diperoleh adalah 1,903 menit.  Hasil penelitian menunjukkan bahwa metode ini dapat digunakan untuk penarikan dan penetapan kadar β-karoten dalam   tanpa pelarut kimia organik.

Incorporation of Cornus mas L. in Soybean Oil: Evaluation of Phytochemical and Antioxidant Activity

2020 ◽  
Vol 16 (3) ◽  
pp. 397-402 ◽  
Author(s):  
Nabi Shariatifar ◽  
Massoud Amanlou ◽  
Ramin Rahimnia ◽  
Shabnam Mahernia ◽  
Amin M. Khaneghah
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Soybean Oil ◽  
Antioxidant Activities ◽  
Beta Carotene ◽  
Dpph Assay ◽  
Synthetic Antioxidant ◽  
Cornus Mas ◽  
Peroxide Values ◽  
Β Carotene

Background: The active compounds of Cornus mas L. were extracted by the aid of water and ethanol and were incorporated into soybean oil samples. Afterward, their antioxidant activities were examined. Methods: The 1, 1 diphenyl-2- picrylhydrazyl (DPPH) and beta-carotene methods were approached in order to assess the antioxidant capacity. Different concentrations of extracts prepared (0,200, 400 and 800 mg/lit) and β hydroxyl toluene (BHT, 100 mg/lit) were added into soybean oil, and the resulted mixtures were incubated for 35 days at 65°C. Peroxide values (PVs) and thiobarbituric acidreactive substances (TBARs) levels were measured each week during the incubation. The collected data from each incubation was analyzed using ANOVA test. Results: Results demonstrated that corresponded values for β-carotene-linoleic acid and DPPH assay of the Cornus mas L. extracted by ethanol and water were lower than synthetic antioxidant and BHT. Conclusion: PVs and TBARs levels of soybean oil during the incubation time was decreased by the incorporation of extracts (water and ethanol) of Cornus mas L. while compared to the control.

Combining Ability of Sweet Potato [Ipomoea batatas (L.) Lam] Genotypes for Beta Carotene and Dry Matter Content in Southern Guinea Savanna, Nigeria

2021 ◽  
Vol 19 (1) ◽  
pp. 53-61
Author(s):  
M.S Afolabi ◽  
G.O Agbowuro ◽  
A.E Salami ◽  
M.O Akoroda
Keyword(s):  
Vitamin A ◽  
Sweet Potato ◽  
Dry Matter ◽  
Beta Carotene ◽  
Diallel Analysis ◽  
Matter Content ◽  
Dry Matter Content ◽  
Carotene Content ◽  
Guinea Savanna ◽  
Β Carotene

Roots of orange fleshed sweet potato varieties currently available in Nigeria contain high quantities of β-carotene or pro-vitamin A but have high moisture content. These varieties have been found to be a cheap and important remedy for vitamin A deficiency. The cream or white fleshed varieties on the other hand, have sweet taste with high dry matter content, giving a dry texture, a quality trait preferred in Nigeria. The objectives of this study were to examine the quantitative inheritance of important traits in sweet potato by means of a diallel analysis with a view to estimating the GCA and SCA components of genetic variance, and to determine the associated type of gene action controlling β-carotene content and root dry mass. A diallel crosses experiment using six parental sweet potato genotypes crossed in all possible combinations were carried out. Resultant thirty progenies were evaluated for beta carotene (β-carotene) and dry matter content in Landmark University, Omu Aran, Kwara State, Nigeria during the cropping season of 2012 and 2013. The 30 F1 progenies along with their parental lines were planted in the same field trial. The trial was laid out in 6 x 6 triple lattice in two replications. Highly significant (P≤ 0.01) differences were observed among the genotypes for all the tested traits. The average β-carotene content for the progenies was 2.86 (mg/100 g f.w.) while the dry matter content was 31.89%. The cross combination 199024.2 x Excel recorded the highest beta carotene (14.37 mg/100 g f.w) and dry matter content (40.10%), this candidate is therefore suggested for further evaluation. Key words: Diallel analysis, Dry matter, Southern Guinea Savanna, Sweet potato, Vitamin A, β-carotene.

REPRODUCTIVE PERFORMANCE OF PREPUBERTAL DAIRY HEIFERS ON LOW OR HIGH β-CAROTENE DIETS

1987 ◽  
Vol 67 (2) ◽  
pp. 477-489
Author(s):  
F. R. TEKPETEY ◽  
W. M. PALMER ◽  
J. R. INGALLS
Keyword(s):  
Vitamin A ◽  
Estrous Cycle ◽  
Reproductive Performance ◽  
Average Daily Gain ◽  
Beta Carotene ◽  
Corn Silage ◽  
Dairy Heifers ◽  
Daily Gain ◽  
Estradiol 17Β ◽  
Β Carotene

Twenty 5-mo-old heifers were assigned randomly to either a no-carotene or a carotene-supplemented grain ration (2–2.5 kg head−1 d−1) for a 10-mo trial to investigate the effect of β-carotene deficiency on pre and postpuberal reproductive performance. All heifers received, ad libitum, a low-carotene basal forage of corn silage, weathered hay and/or straw. Mean serum β-carotene and vitamin A were higher (P < 0.05) in the carotene-supplemented heifers. Beta-carotene supplementation improved (P < 0.05) overall average daily gain, but had no effect on mean age and body weight at puberty, estrous cycle length and services per conception. Mean serum concentrations of progesterone (P4) estradiol-17β, LH and FSH from prepuberty to day 17 of the first estrous cycle were not affected by β-carotene supplementation. Total P4 output during the estrous cycle did not differ between control and β-carotene-supplemented heifers. However, intensity of estrus was weaker (P < 0.01) and interval (h) from LH peak to ovulation was longer (P < 0.01) in the control (low-carotene) heifers. Key words: β-carotene, vitamin A, reproductive performance, prepubertal dairy heifers

scholarly journals β-Cryptoxanthin- and α-carotene-rich foods have greater apparent bioavailability than β-carotene-rich foods in Western diets

2010 ◽  
Vol 105 (2) ◽  
pp. 212-219 ◽  
Author(s):  
Betty J. Burri ◽  
Jasmine S. T. Chang ◽  
Terry R. Neidlinger
Keyword(s):  
Vitamin A ◽  
Reversed Phase ◽  
The Body ◽  
Provitamin A ◽  
Food Sources ◽  
Dietary Intakes ◽  
Reversed Phase Hplc ◽  
Food Record ◽  
Apparent Bioavailability ◽  
Β Carotene

β-Carotene (BC), β-cryptoxanthin (CX) and α-carotene (AC) are common carotenoids that form retinol. The amount of retinol (vitamin A) formed from carotenoid-rich foods should depend chiefly on the bioavailability (absorption and circulation time in the body) of carotenoids from their major food sources and the selectivity and reactivity of carotene cleavage enzymes towards them. The objective of the present study was to estimate the apparent bioavailability of the major sources of provitamin A (AC, BC and CX) from the diet by comparing the concentrations of these carotenoids in blood to their dietary intakes. Dietary intakes were estimated by FFQ (three studies in this laboratory, n 86; apparent bioavailability calculated for six other studies, n 5738) or by food record (two studies in our laboratory, n 59; apparent bioavailability calculated for two other studies, n 54). Carotenoid concentrations were measured by reversed-phase HPLC. Apparent bioavailability was calculated as the ratio of concentration in the blood to carotenoid intake. Then apparent bioavailabilities for AC and CX were compared to BC. Eating comparable amounts of AC-, CX- and BC-rich foods resulted in 53 % greater AC (99 % CI 23, 83) and 725 % greater CX (99 % CI 535, 915) concentrations in the blood. This suggests that the apparent bioavailability of CX from typical diets is greater than that of BC. Thus, CX-rich foods might be better sources of vitamin A than expected.

Fatty acid content in bovine milk under the action of β-carotene

2021 ◽  
Vol 12 (2) ◽  
Author(s):  
L Shevchenko ◽  
◽  
V Mykhalska ◽  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Vitamin A ◽  
Milk Fat ◽  
Unsaturated Fatty Acids ◽  
Bovine Milk ◽  
Feed Additives ◽  
The Body ◽  
Biologically Active ◽  
Β Carotene

One of the key issues in the production of high-quality, biologically complete milk is to provide cows with a sufficient amount of biologically active substances, including vitamin A and its precursor β-carotene. One of the ways to enrich milk with vitamin A and carotenoids is feeding cows with natural feed additives, including vitaton containing up to 10% trans-β-carotene, which can be converted into vitamin A, as well as exhibit antioxidant, immunostimulating, and anticarcinogenic effects in the body. Vitaton is the biomass of fungi Blakeslea trispora TKST strain, a product of microbial synthesis obtained by cultivating the producer on the waste of starch and molasses production. In terms of sanitation, the biomass of the fungi Bl. trispora is safe for animals, since the main condition for its production is sterility, excluding any microorganisms entering the fermentation medium. Besides, after the end of fermentation, the temperature of the medium is raised to 65–70 °C in the fermenter that destroys the β-carotene producer itself. We conducted research on cows of the Ukrainian black and white dairy breed, which were fed with vitaton, in conditions of “Kuibysheve” ALLC of the Poltava region. The introduction of vitaton into the cows’ diet contributes to the increase of the milk fat content by an average of 0.26% and enhances the phospholipid synthesis in the mammary gland by 1.8 times. Vitaton as a biologically active additive did not affect the ratio of total saturated to unsaturated fatty acids in bovine milk but stimulated the formation and inclusion of two unsaturated fatty acids into the milk fat, namely nonadecanoic and α-linolenic, against the background of the disappearance of the arachidonic acid peak in the chromatogram. Changes in the fatty acid composition of milk fat obtained from cows fed with the vitaton as a source of β-carotene indicate the effect of β-carotene and other biologically active components contained in vitaton on milk lipid synthesis.

scholarly journals The Role of Beta-carotene Metabolism in Maternal Cardiac Remodeling: Findings in Mice Lacking Beta-carotene 9′,10′-oxygenase (BCO2) (FS06-04-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Chelsee Holloway ◽  
You-Kyung Kim ◽  
Loredana Quadro
Keyword(s):  
Vitamin A ◽  
Cardiac Hypertrophy ◽  
Mrna Expression ◽  
Active Form ◽  
Beta Carotene ◽  
Regulatory Genes ◽  
Chow Diet ◽  
Mrna Levels ◽  
Β Carotene

Abstract Objectives High intake of fruits and vegetables, main vitamin A sources, is associated with improved cardiac function. β-carotene, the most abundant dietary precursor of vitamin A, is cleaved by β-carotene 15,15′-oxygenase (BCO1) and β-carotene 9′,10′-oxygenase (BCO2). However, BCO2 is the only β-carotene cleavage enzyme expressed in adult hearts. Cardiac mRNA levels of Bco2 are elevated at mid-gestation in wild-type (WT) mice when the heart is hypertrophic. In the absence of BCO2 (Bco2-/- mice) the maternal heart fails to enlarge. Therefore, we aim to elucidate the role of BCO2 in maternal cardiac hypertrophy and to determine if metabolic pathways in the heart are disrupted by loss of BCO2. We hypothesize that BCO2 contributes to maternal cardiac hypertrophy by affecting homeostasis of RA, the active form of vitamin A. Methods Age matched WT and Bco2-/- (KO) mice raised on a chow diet were sacrificed at 14.5 days pregnant. Cardiac mRNA and protein expression of retinoid and lipid regulatory genes were measured. HPLC and LC/MS detected cardiac retinoids (vitamin A and its derivatives) levels. Results Pregnancy (mid-gestation) is associated with cardiac RA deficiency in WT dams. KO mice already showed cardiac RA deficiency pre-pregnancy. KO female mice have reduced PDK4 mRNA expression and enhanced PDH activity (phosphorylation) in the heart, that is reversed earlier, at mid-gestation. KO mice have increased cardiac Glut1 mRNA expression and reduced triglyceride levels. Lipid regulatory genes such as Pgc1a and Scd1 are increased at mid-pregnancy in the heart of WT dams but not in KO mothers. Conclusions Our data indicates that RA may be involved in modulating the cardiac hypertrophy of pregnancy. Dysregulation of RA homeostasis in the heart of KO females results in metabolic adaptations that makes the heart of the non-pregnant females preferentially dependent on glucose as an energy source. During pregnancy retinoic deficiency in the KO heart may induce an earlier attenuation of PDH activity, facilitating utilization of fat over glucose as energy substrate. These and other findings support the hypothesis of a crucial role of BCO2 in regulating heart hypertrophy, at least in females. Funding Sources NIH/NHLBI F31.

scholarly journals UJI KANDUNGAN VITAMIN A TANAMAN WORTEL (Daucus Corata L.) DI DESA NGABAB KABUPATEN MALANG

2021 ◽  
Vol 3 (2) ◽  
pp. 70-77
Author(s):  
Beta Herilla Sekti ◽  
Rakhmadani Gadis Aprilianti ◽  
Susi Wijiastini
Keyword(s):  
Quantitative Analysis ◽  
Vitamin A ◽  
Daucus Carota ◽  
Sampling Technique ◽  
Beta Carotene ◽  
The Body ◽  
Comparison Standard ◽  
Daucus Carota L ◽  
Carrot Plant ◽  
Carrot Extract

Carrot plant (Daucus carota L.) is a type of vegetable that contains lots of vitamin A and is very much needed by the body to help regulate or metabolic processes in the body. The research design used in this study was experimental, the population and sample were carrots from Ngabab village aged 3-3.5 months, using purposive sampling technique. The results of the qualitative analysis showed that there were orange spots with an Rf value of 0.64 in the carrot extract and the beta-carotene comparison standard. The results of the quantitative analysis showed that the average level of vitamin A in carrots was 49.7% with an absorbance of 0.649. Keywords: Visible Spectrophotometry, Vitamin A, Carrot.

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