scholarly journals Role Of Moringa Oleifera Leaf Extract In Increasing Hemoglobin Levels In Pregnant Rats With Anemia

2021 ◽  
Vol 14 (1) ◽  
pp. 8-13
Author(s):  
Reny Retnaningsih ◽  
Rani Safitri
Keyword(s):  
Leaf Extract ◽  
Moringa Oleifera ◽  
Positive Control ◽  
Negative Control ◽  
Female Rats ◽  
Control Group ◽  
Control Group Design ◽  
Pregnant Rats ◽  
Nutritional Problem ◽  
Post Test

Anemia is one of the epidemic health problems in society and the most common nutritional problem. The Moringa oleifera is rich in excellent dietary content and can complement the body's needs. This study aims to determine Moringa oilefera leaf extract's effectiveness in increasing hemoglobin levels in pregnant rats (Ratus norvegicus) with anemia. This paper used a true experimental research design with a pre-post test randomized control group design. This study utilized female rats (Ratus norvegicus), pregnant 8-12 weeks, weighing 200 grams. Experimental procedures were four treatments with three repetitions. Types of treatment were negative control and positive control (administration of Moringa oilefera leaf extract) at a dose of 0.18 g / head, 0.36 g / head, and 0.72 g/head. Analysis using one way ANOVA with an error rate of 0.05. The results showed p<0.005, which means a significant increase in hemoglobin levels in the group given the treatment of moringa leaf extract at a dose of 0.72 g/head/day. The conclusion and suggestion from this research are public could get information about using natural materials as economically valuable drugs (back to nature)

scholarly journals Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

2018 ◽  
Vol 5 (1) ◽  
pp. 58
Author(s):  
Noer Kumala Indahsari ◽  
Masfufatun Masfufatun ◽  
Emilia Devi D.R
Keyword(s):  
Treatment Group ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

scholarly journals Uji daya hambat ekstrak daun bayam petik (Amaranthus hybridus L.) terhadap pertumbuhan bakteri Staphylococcus aureus

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Oktovianus Pormes ◽  
Damajanty H.C. Pangemanan ◽  
Michael A. Leman
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Amaranthus Hybridus ◽  
Group Design ◽  
Post Test

Abstract: Synthetic antibiotics have certain side effects, therefore, it is necessary to find alternative natural antibacterial materials which is easily available and to be cultivated, inter alia Amaranthus hybridus L. Its leaves contain active compounds, so they might have antibacterial potential. This study was aimed to determine the inhibitory effect of Amaranthus hybridus leaf extract on the growth of Staphylococcus aureus. This was a true experimental study using post test only control group design. This study was conducted at the Microbiology Laboratory of Faculty of Medicine and the Natural Phytochemical Laboratory of Faculty of Mathematics at Sam Ratulangi University. The modified Kirby-Bauer method was used with three wells, containing Amaranthus hybridus leaf extract, the positive control, and the negative control; and with 5 repetitions. The results showed that the average diameters of the inhibition zone of Amaranthus hybridus leaf extract and of the negative control were 0 mm meanwhile of erythromycin as the positive control was 38.8 mm. Conclusion: Amaranthus hybridus leaf extract had no inhibitory effect on Staphylococcus aureus. Keywords: inhibitory zone, Amaranthus hybridus L, Staphylococcus aureus Abstrak: Bahan antibiotik sintetik memiliki efek samping, sehingga perlu dicari bahan alternatif yaitu bahan alami yang mudah didapat dan dibudidayakan, salah satunya ialah bayam petik (Amaranthus hybridus L.). Daun bayam petik memiliki potensi antibakteri karena memiliki kandungan senyawa aktif. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun bayam petik terhadap pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Penelitian dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran dan Labaratorium Fitokimia Fakultas MIPA Unsrat. Metode pengujian yang digunakan ialah modifikasi Kirby-Bauer dengan menggunakan tiga buah sumuran yang diberi ekstrak daun bayam petik, kontrol positif, dan kontrol negatif, sebanyak 5 kali pengulangan. Hasil penelitian menunjukan diameter rerata dari zona hambat yang terbentuk pada sumur dengan ekstrak daun bayam petik dan pada sumur dengan kontrol negatif ialah 0 mm, sedangkan pada sumur yang diberi kontrol positif amoksisilin terjadi resistensi sehingga diganti dengan eritromisin dan didapatkan rerata zona hambat ialah 38,8 mm. Simpulan: Ekstrak daun bayam petik (Amaranthus hybridus L.) tidak memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus.Kata kunci: daya hambat, Amaranthus hybridus L, Staphylococcus aureus

scholarly journals EFIKASI EKSTRAK DAUN ZODIA (Evodia suaveolens) PELARUT HEKSANA DALAM MEMATIKAN LARVA INSTAR III Aedes aegypti

2020 ◽  
Vol 8 (2) ◽  
pp. 195
Author(s):  
Didi Setiyadi ◽  
Martini Martini ◽  
Mochamad Hadi
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Larval Mortality ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Environmental Damage ◽  
P Value ◽  
Control Group Design ◽  
Post Test

DHF is a disease caused by a virus which is transmitted through the bite of the Aedes aegypti mosquito and is one of the health problems in Indonesia. Efforts to decide the transmission of DHF by controlling the population of Aedes aegypti at the larval stage using larvaside temepos. Long-term use of temephos causes Aedes aegypti resistance and environmental damage. One of the plants that has the potential as botanical larvaside is the leaves of zodia (Evodia suaveolens). The purpose of this study was to prove the efficacy of zodia leaves of hexane solvent for killing A. aegypti larvae. This type of research is a pure experiment with the post test only control group design. The research sample is A. aegypti stage III susceptible strain. There are 7 groups consisting of 1 negative control (aquades), positive control (1 ppm temepos) and 5 treatment groups (zodia leaf extract). Data on larval mortality were counted 24 hours after treatment. The results of the one way ANOVA test showed that there were differences in mortality between test groups with a p value: 0.001 α (0.05). Zodia leaf extract probit test results showed LC50-24 hour and LC90-24 hour values of 0,443 ppm and 0,788 ppm. Zodia leaf extract with hexane solvent has efficacy as Aedes aegypti larvicide.

scholarly journals UJI EFEKTIFITAS BIOLARVASIDA EKSTRAK ETANOL BUAH LAMPESU (Baccaurea lanceolata) TERHADAP LARVA INSTAR III Culex quinquefasciatus

2020 ◽  
Vol 3 (3) ◽  
pp. 7
Author(s):  
Dea Alfani Nandjan
Keyword(s):  
Culex Quinquefasciatus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Chemical Insecticide ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Infection Disease

Filariasis is chronic infection disease caused by worm and carried by Culex quinquefasciatus mosquito. One of theways to controlling mosquitoes vectors are use biolarvacide or chemical insecticide. Chemical insecticide causingresistance Culex quinquefasciatus larvae and toxic for human. This research aim to determine the biolarvacideeffectiviteness of ethanol extract lampesu fruit (Baccaurea lanceolata) to Culex quinquefasciatus larvae instars III.This study was true experimental with a Post test-only control group design. This study used 700 larvae instars III ofCulex quinquefasciatus divided into 7 groups consentration of 0,2%, 0,4%, 0,6%, 0,8% and 1%, the positive control(abate) and negative control (aquadest). The observation was did after treatment in 3 hours, 6 hours, 12  hours and 24hours. The experiment is replicated four times. At 24 hours exposure concentration of 0,6% the test larvae mortalityreached 38% and at concentration of 1% the test larvae mortality reached 30%. In this stuy the concentration of1,531 % was effective to kill larvae with of 50% mortality(LC50) and concentration of 10,729 % was effective to killlarvae with of 90% mortality(LC90). Ethanol Ekstract of Lampesu Fruit (Baccaurea lanceolata) not effective asbiolarvacide ofCulex quinquefasciatus larvae instars III.

scholarly journals EFEKTIVITAS EKSTRAK DAUN JERUK PURUT (Citrus hystrix) SEBAGAI INSEKTISIDA ALAMI TERHADAP KECOAK AMERIKA (Periplaneta americana) DENGAN METODE SEMPROT

2020 ◽  
Vol 8 (1) ◽  
pp. 8-14
Author(s):  
Hanina Hanina ◽  
Sarah Madeleyne Baringbing
Keyword(s):  
Leaf Extract ◽  
Periplaneta Americana ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Observation Time ◽  
American Cockroach ◽  
Control Group ◽  
Significant Difference ◽  
Post Test

ABSTRACT Cockroach control can be done in various ways one of which is spraying insecticide. Natural insecticides are less dangerous than conventional insecticides. This study aimed to determined the effectiveness of kaffir lime leaf extract (Citrus hystrix) as a natural insecticide against cockroach americana (Periplaneta americana) with spray method. This research was an experimental study with a post-test only control group designed design. The sample in this study was 60 cockroaches, the sample was divided into 6 groups: negative control (aquades), positive control (transflutrin 0.06%) and and 4 test groups with concentrations of kaffir lime leaf extract (20%, 40%, 60% , and 80%) each group consisted of 10 cockroaches. Phytochemical tests were carried out to determine the content of secondary metabolites in extracts of kaffir lime leaves. Positive phytochemical test results containing flavonoids, alkaloids and essential oils. Based on probit analysis, it is known that the LC50 value = 58.5%; LC95 = 85.1%. The time of death of LT50 is fastest at a concentration of 60% (3,036 hours) and LT95 is the fastest at a concentration of 80% (5,960 hours). Anova test analysis results showed that the positive control group had no significant difference with kaffir lime leaf extract concentration of 80% Kaffir lime leaf extract has an insecticide effect, the higher the concentration of kaffir lime leaf extract, the more number of dead cockroaches and the longer the observation time, the number of cockroaches death will also increase. Keywords: American cockroach, kaffir lime leaf extract, cockroach death   ABSTRAK Pengendalian kecoa dapat dilakukan dengan berbagai cara salah satunya penyemprotan insektisida. Insektisida alami kurang berbahaya dibanding insektisida konvensional. Penelitian ini bertujuan untuk mengetahui efektifitas ekstrak daun jeruk purut (Citrus hystrix) sebagai insektisida alami terhadap kecoak americana (Periplaneta americana) dengan metode semprot. Jenis penelitian ini adalah penelitian eksperimental dengan rancangan post-test only control group designed. Sampel dalam penelitian ini adalah 60 ekor kecoak, sampel dibagi menjadi 6 kelompok yaitu kontrol negatif (aquades), kontrol positif (transflutrin 0,06%) dan dan 4 kelompok uji dengan konsentrasi ekstrak daun jeruk purut (20%, 40%, 60%, dan 80%) masing-masing kelompok terdiri dari 10 ekor kecoak. Uji fitokimia dilakukan untuk mengetahui kandungan senyawa metabolit sekunder pada ekstrak daun jeruk purut. Hasil uji fitokimia positif mengandung flavonoid, alkaloid dan minyak atsiri. Berdasarkan analisis probit, diketahui nilai LC50 = 58,5%; LC95 = 85,1%. Waktu kematian LT50 paling cepat pada konsentrasi 60% (3,036 jam) dan LT95 paling cepat pada konsentrasi 80% (5,960 jam). Hasil analisis uji anova menunjukkan bahwa kelompok kontrol positif tidak terdapat perbedaan yang signifikan dengan ekstrak daun jeruk purut konsentrasi 80% Ekstrak daun jeruk purut memiliki efek insektisida, semakin tinggi konsentrasi ekstrak daun jeruk purut maka jumlah kecoak yang mati semakin banyak dan semakin lama waktu pengamatan, maka jumlah kecoak yang mati juga akan semakin banyak. Kata kunci : kecoak amerika, ekstrak daun jeruk purut, kematian kecoak

scholarly journals Efektivitas perasan daun seledri (Apium graveolens Linn.) sebagai pembersih gigi tiruan terhadap pertumbuhan Candida albicans pada basis gigi tiruan nilon termoplastikThe effectiveness of celery leaf juice (Apium graveolens Linn.) as a denture cleaner against the growth of Candida albicans on thermoplastic nylon denture base

2020 ◽  
Vol 32 (3) ◽  
pp. 184
Author(s):  
Dhilan Purna Aji ◽  
Achmad Gunadi ◽  
Tantin Ermawati
Keyword(s):  
Candida Albicans ◽  
Positive Control ◽  
Negative Control ◽  
Apium Graveolens ◽  
Control Group ◽  
Control Group Design ◽  
Denture Base ◽  
Significant Difference ◽  
Post Test ◽  
Leaf Juice

Pendahuluan: Basis gigi tiruan nilon termoplastik merupakan alternatif pengganti basis gigi tiruan resin akrilik. Pembersihan gigi tiruan diperlukan untuk menjaga basis gigi tiruan agar tetap bersih dari kontaminasi mikroorganisme. Daun seledri merupakan bahan alam yang memiliki kandungan flavonoid, saponin, tanin, dan minyak atsiri yang sudah terbukti bersifat antijamur. Penelitian sebelumnya hanya melihat zona hambat saja. Tujuan penelitian adalah menganalisis efektivitas perasan daun seledri sebagai pembersih gigi tiruan terhadap pertumbuhan Candida albicans pada basis gigi tiruan nilon termoplastik. Metode: Eksperimental laboratoris dengan rancangan penelitian post-test only control group design. Sampel nilon termoplastik berbentuk cakram dengan diameter 10 mm dan tebal 2 mm  sebanyak 25 dibagi menjadi 5 kelompok. Sampel dikontaminasi dengan C. albicans setelah direndam dalam kontrol negatif (akuades) selama 6 jam, kontrol positif (tablet effervescent) selama 15 menit, dan perasan daun seledri konsentrasi 25, 50, dan 100% selama 6 jam. Sampel selanjutnya diletakkan dalam media sabouraud dextrose broth (SDB). Pengujian efektivitas perasan daun seledri dilakukan dengan menentukan nilai absorbansi kekeruhan media SDB menggunakan spektrofotometer. Data dianalisis menggunakan one-way ANOVA dan Least Significant Difference (LSD). Hasil: Nilai konsentrasi C. albicans pada kelompok kontrol negatif mendapatkan hasil 0,380 x 108 CFU/mL, kontrol positif mendapatkan hasil 0,310 x 108 CFU/mL, perasan daun seledri konsentrasi 25% mendapatkan hasil 0,243 x 108 CFU/mL, konsentrasi 50% mendapatkan hasil 0,160 x 108 CFU/mL, dan konsentrasi 100% mendapatkan hasil 0,236 x 108 CFU/mL. Simpulan: Perasan daun seledri efektif sebagai pembersih gigi tiruan untuk menghambat  pertumbuhan C. albicans, dengan konsentrasi yang efektif sebesar 50%.Kata kunci: Nilon termoplastik, perasan daun seledri, Candida albicans. ABSTRACTIntroduction: Thermoplastic nylon denture base is an alternative to the acrylic resin denture base. Denture cleaning is needed to keep the denture base clean from contamination by microorganisms. Celery leaves are natural ingredients that contain flavonoids, saponins, tannins, and essential oils that have been proven to have antifungal properties. Previous research only observed the inhibitory zone. This research was aimed to analyse the effectiveness of celery leaf juice as a denture cleaner against the growth of Candida albicans on thermoplastic nylon denture base. Methods: Experimental laboratory with a post-test only control group design was conducted towards 25 disc-shaped thermoplastic nylon samples with a diameter of 10 mm and a thickness of 2 mm, and divided into five groups. Samples were contaminated with C. albicans after immersion in the negative control (aquadest) for 6 hours, positive control (effervescent tablets) for 15 minutes, and celery leaf juice with the concentrations of 25, 50, and 100% for 6 hours. The sample was then placed in the Sabouraud-dextrose broth (SDB) medium. The effectiveness of celery leaf juice was tested by determining the absorbance value of SDB media turbidity using a spectrophotometer. Data were analysed using one-way ANOVA and Least Significant Difference (LSD). Results: The concentration value of C. albicans in the negative control group was 0.380 x 108 CFU / mL, the positive control was 0.310 x 108 CFU / mL, the juice of celery leaves with the concentration of 25% was 0.243 x 108 CFU / mL, the concentration of 50% was 0.160 x 108 CFU / mL, and the concentration of 100% was 0.236 x 108 CFU / mL. Conclusion: Celery leaf juice is effective as a denture cleaner to inhibit the growth of C. albicans, with an effective concentration of 50%.Keywords: Thermoplastic nylon, celery leaf juice, Candida albicans.

scholarly journals Pengaruh Pemberian Ekstrak Etanol Buah Masisin (Rhodomyrtus tomentosa Wight) terhadap Frekuensi, Konsistensi, dan Durasi Diare pada Hewan Coba Mencit Putih Jantan (Mus musculus) yang Diinduksi Oleum Ricini

2019 ◽  
Vol 5 (1) ◽  
pp. 22-40
Author(s):  
Angeline Novia Toemon ◽  
Austin Bertilova Carmelita ◽  
Astri Widiarti ◽  
Dian Mutiasari
Keyword(s):  
Mus Musculus ◽  
Positive Control ◽  
Negative Control ◽  
Study Data ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Ethanol Extracts ◽  
One Way Anova ◽  
Rhodomyrtus Tomentosa

People in Central Borneo which is often used to treat diarrhea is the fruit of masisin (Rhodomyrtus tomentosa Wight) with active compounds which are capable to treat diarrhea such as alkaloids, flavonoids, tannins, saponins, and steroids. Diarrhea is a defecate with a liquid or half-liquid feces, the content of water in a feces is more than usual, it is more than 200 g or 200 ml / 24 hours. To know the effectiveness of the extract of the fruit of masisin as an alternative to antidiarrheal treatment seen from the frequency, duration, and consistency of feces in male white mice (Mus musculus). This study design was a true experimental design with post-test control group design with 5 treatments for each group. This study started by making some extracts of Masisin fruit. Followed observation with 5 treatment groups animals. The induction of diarrhea was using oleum ricini, with aquadest as the negative control, and Loperamide HCL as the positive control. The dose of ethanol extracts of masisin fruit divided into 0.3 mg/g BW, 0.6 mg/g BW and 1.2 mg/g BW, all of the treatments were given orally. This study data was statistically analyzed with one way ANOVA. The results of one way ANOVA test showed that the value of p <0,05 meaning that there is the influence of EEBM on frequency, consistency and duration of diarrhea at doses 0.3 mg/g BW, 0.6 mg/g BW and 1.2 mg/Gbw. Based on observations ethanol extracts of masisin fruit had antidiarrheal effect at doses 0,3 mg/g BW, 0.6 mg/g BW and 1.2 mg/g BW in male white mice (Mus musculus).

scholarly journals Pengaruh Pemberian Ekstrak Rosella (Hibiscus sabdariffa Linn) Terhadap Kadar Malondialdehid dan Aktivitas Katalase Tikus yang Terpapar Karbon Tetraklorida

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Zuraida Zuraida ◽  
Eti Yerizel ◽  
Eliza Anas
Keyword(s):  
Vitamin C ◽  
Negative Control Group ◽  
Organ Damage ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Hibiscus Sabdariffa ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Abstrak Pemberian rosella (Hibiscus sabdariffa Linn) diharapkan melindung hepar tikus dari kerusakkan akibat stres oksidatif pada keracunan karbon tetraklorida (CCl 4). Senyawa yang sering dijadikan petunjuk adanya kerusakan tersebut adalah malondialdehid (MDA). Rosella mengandung vitamin C, flavonoid, polifenol dan beta karoten. Tujuanpenelitian ini adalah menentukan pengaruh pemberian ekstrak rosella (Hibiscus sabdariffa Linn) terhadap MDA dan aktivitas katalase tikus yang terpapar CCl 4. Ini adalah penelitian eksperimental dengan desain Post test Only Control Group Design. Sampel 24 ekor tikus Strain Wistar berumur 2-3 bulan, berat 150-200 gr. Sampel diambil secara acakdan dibagi 4 kelompok terdiri dari kelompok kontrol negatif, kontrol positif (CCl 4), perlakuan 1 (CCl 4 dan ekstrak rosella 250 mg/kg bb) dan perlakuan 2 (CCl4  dan ekstrak rosella 500 mg/kg bb). Pemberian CCl 4secara oral dosis tunggal, setelah 24 jam kemudian diberi ekstrak rosella secara oral selama 14 hari. Data dianalisis dengan uji Anova, tingkatkepercayaan 95%.Pemberian ekstrak rosella secara statistik didapatkan perbedaan yang signifikan rerata kadar MDA dan katalase antar kelompok (p < 0,05). Disimpulkan bahwa ekstrak rosella dapat menurunkan kadar MDA dan meningkatkan aktivitas katalase tikus yang terpapar CCl 4. Kata kunci: karbon tetraklorida, MDA, katalase, rosella Abstract Administering roselle (Hibiscus sabdariffa Linn) is expected to protect rat liver from damage caused by oxidative stress in CCl4 poisoning. Rosella contains vitamin C, flavonoids, polyphenol and beta carotene. Compounds which was often used as marker of the damage caused by free radicals wa MDA. The objective of this study was to determine the effect of extracts of roselle (Hibiscus sabdariffa Linn) on MDA and catalase activity of rats exposed to CCl4. Experimental research design with Post test Only Control Group Design. Samples of 24 male Wistar Strain rats were 2-3 months old. weighing 150-200 gr. Samples were taken at random and divided into 4 groups consisting of a negative control group, positive control (CCl4), treatment 1 (CCl4 and roselle extract 250 mg / kg bw) and treatment 2 (CCl4 and roselle extract 500 mg / kg bw). CCl4 was given a single dose orally, after 24 hours, the subjects were given rosella extract orally for 14 days. Data were analyzed by ANOVA with a confidence level of 95%. Rosella extract obtained statistically significant differences of MDA and catalase levels among groups (p <0.05). It can be concluded that the rosella extract can reduce levels of MDA and increase the activity of catalase mice exposed to CCl4. From the research it can be concluded that the rosella extract can reduce levels of MDA and increas the activity of catalase mice exposed to CCl4. It is needed further research on the toxicity of extracts of rosella and organ damage caused.Keywords: carbon tetrachloride, MDA, catalase, rosella

scholarly journals Effect Of Garlic Extract (Allium Sativum Linn) in Healing Wistar Rats Wound Through Assesment Of Collagen

2021 ◽  
Vol 1 (1) ◽  
pp. 1-5
Author(s):  
Wa Ode Megasari ◽  
Irfan ◽  
Yulia Y.Djabi
Keyword(s):  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Garlic Extract ◽  
Control Group ◽  
Research Subjects ◽  
Time Dimension ◽  
Control Group Design ◽  
Post Test ◽  
Test Control

Baground: The severity of injuries is influenced by the wound surface area and structure of damaged tissue include skin tissue , blood vessels , nerves , muscles , bones to internal organs. The aim of this research is to determine the effect of 10 % concentration of garlic extract in healing wistar rats wound through assessment of collagen. Method: This research method is laboratory experimental study was conducted using the Randomized Post Test Control Group design with 45 wistar rats as the research subjects. The rats were divide into three groups; negative control, positive control and treatment groups. Each group was then divide based on the time dimension: 3rd, 7th, 14th day. The resulth were analysed using the Post Hoc Way Anova and Kruskall Wallis tests. Results: The result showed aquick decrease in collagen in the group treated with garlic extract. The level of decrease was not significant compared with the negative and positive control group. Conclusions: The study confirms that garlic extract contribute to the healing of wound although the comparison of change towards control group did not show a significant rate Keywords: Garlic Extract, collagen   Pendahuluan: Tingkat keparahan luka dipengaruhi oleh luas permukaan luka dan struktur jaringan yang rusak meliputi jaringan kulit, pembuluh darah, saraf, otot, tulang sampai organ dalam. Penelitian ini bertujuan untuk mengetahui pengaruh salep ekstrak bawang putih dengan konsentrasi 10% terhadap proses penyembuhan luka tikus wistar dengan model perlukaan akut melalui penilaian kolagen. Metode: Penelitian ini merupakan penelitian eksperimental laboratoris dengan menggunakan desain Randomized Post Test Control Group yang menggunakan tikus Wistar sebagai subyek penelitian. Penelitian ini menggunakan 45 tikus wistar yang dibagi dalam 3 kelompok yaitu kontrol negatif, kontrol positif,dan perlakuan. Selanjutnya masing-masing kelompok dibagi 3 berdasarkan dimensi waktu yakni hari ke-3, hari ke-7, dan hari ke-14. Hasil: Hasil penelitian menunjukkan kepadatan Kolagen pada kelompok yang diberi ekstrak bawang putih mengalami kepadatan kolagen dari hari ke hari . Namun, derajat kepadatan yang ditimbulkan tidak menunjukkan nilai yang bermakna jika dibandingkan dengan kelompok kontrol negatif dan kontrol positif. Kesimpulan: salep ekstrak bawang putih dapat mempercepat penyembuhan luka, tetapi perbandingan perubahannya terhadap kelompok kontrol tidak menunjukkan nilai yang signifikan Kata kunci: Ekstrak bawang putih, kolagen

scholarly journals Uji Aktivitas Ekstrak Daun Pirdot (Sauraia vulcani Korth.) Terhadap Pertumbuhan Candida albicans Secara In Vitro

2020 ◽  
Vol 11 (1) ◽  
pp. 1
Author(s):  
Romauli Anna Teresia Marbun
Keyword(s):  
Candida Albicans ◽  
Dimethyl Sulfoxide ◽  
Leaf Extract ◽  
Skin Lesions ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Post Test ◽  
True Experiment

(Article History: Received 5 October 2020; Revised 8 October 2020; Accepted 15 October 2020) ABSTRAKJamur Candida albicans merupakan jamur penyebab kandidiasis dan penyebab sariawan, lesi pada kulit, vulvavaginistis, candida pada urin (kandiduria), dan gastrointestinal kandidiasis. Salah satu jenis tanaman yang berkhasiat sebagai obat yaitu pirdot (Sauraia vulcani Korth.) dan bagian yang biasanya digunakan adalah bagian daun. Tujuan penelitian ini adalah untuk mengetahui aktivitas ekstrak daun pirdot terhadap pertumbuhan C. albicans secara in vitro. Penelitian ini merupakan penelitian true experiment dengan rancangan penelitian post-test only control group. Metode yang digunakan adalah difusi agar menggunakan teknik kirby bauer. Penelitian ini menggunakan 4 variasi konsentrasi ekstrak daun pirdot, yaitu 10%, 20%, 40%, dan 80%, kontrol positif (tablet ketokonazol), dan kontrol negatif (Dimethyl Sulfoxide). Data yang diperoleh dianalisa menggunakan statistika secara deskriptif. Hasil penelitian menunjukkan daya hambat mulai dari konsentrasi 10% sebesar 8,96 mm daya hambat tertinggi pada konsentrasi 80% sebesar 18,33 mm. Kesimpulan ekstrak daun pirdot memiliki aktivitas antijamur terhadap C. albicans.Kata kunci: Antijamur; Candida albicans; Metode Difusi; Saurauia vulcani Korth. ABSTRACTCandida albicans is fungus that causes candidiasis and causes thrush, skin lesions, vulvavaginism, candidiuria and gastrointestinal candidiasis. One type of plant that has medicinal properties is pirdot (Sauraia vulcani Korth.) and the part that is usually used is the leaves. The purpose of this study was to determine the activity of pirdot leaf extract on the growth of C. albicans in vitro. This study was a true experiment with a post-test only control group research design. The method used agar diffusion using Kirby Bauer technique. This study used 4 variations concentration namely 10%, 20%, 40%, and 80%, positive control (ketoconazole tablets), and negative control (Dimethyl Sulfoxide). The data obtained were analyzed using descriptive statistical analysis. The results showed that the inhibitory from a concentration of 10% of 8.96 mm, the highest inhibition was at 80% of 18.33 mm. In conclusion, pirdot leaf extract has antifungal activity against C. albicans.Keywords: Antifungal; Candida albicans; Diffusison Method; Saurauia vulcani Korth

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