scholarly journals Development of promiscous rhizobia for diverse rabi legumes (Chickpea, Pea and Lentil)

2017 ◽  
Vol 9 (1) ◽  
pp. 215-221 ◽  
Author(s):  
Ikbal Ikbal ◽  
Mukesh R Jangra ◽  
K. M. Sarim ◽  
V.K. Sikka
Keyword(s):  
Nitrogen Content ◽  
Dry Weight ◽  
Symbiotic Association ◽  
Bacterial Strains ◽  
Fresh Weight ◽  
Cross Combination ◽  
Selectable Markers ◽  
Farming Community ◽  
Rhizobium Strains ◽  
Strict Specificity

Conjugation between microsymbiont was used to create genetic variations in rhizobia for diverse rabi legumes (chickpea, pea and lentil) with better characteristics in nodulation and nitrogen fixation process. Ten antibiotics were used as selectable markers for the screening of twenty four bacterial strains to be used in mating experiments for obtaining transconjugants. All bacterial strains were sensitive to gentamycin and resistant to streptomycin, kanamycin and sulphanilamide. Total five fusants were obtained from each rhizobial cross combination with the help of electro-poration. Modified transconjugants, rhizobial strains had promiscuous infection with 50-122% more nodules showed significant increase in shoot fresh weight, dry weight and total nitrogen content in chickpea, pea and lentil plants. Electrofusantsrhizobial strains improved shoot nitrogen content up to 67% in lentil and 54% in pea and chickpea plants. The amount of nitrogen fixed in chickpea was highest (3.71gm) by transconjugants DP-C6- HLN followed by DP-C6-HP14 (3.56gm). Transconjugants DP-HP14-HLN fixed the highest amount of nitrogen (3.92gm) in pea and 4.06gm in lentil plants. Plasmids were also analyzed in order to characterize their role in the evolution of rhizobial symbionts and their involvement in symbiotic behaviour. The developed Rhizobium strains with improved symbiotic association and ability to infect across strict specificity for host legumes would be of great help for the farming community at large.

scholarly journals Distribution of Growth and Enzyme Activity in the Developing Grain of Wheat

1961 ◽  
Vol 14 (4) ◽  
pp. 552 ◽  
Author(s):  
AHGC Rijven ◽  
R Cohen
Keyword(s):  
Enzyme Activity ◽  
Nitrogen Content ◽  
Total Nitrogen ◽  
Dry Weight ◽  
Total Nitrogen Content ◽  
Fresh Weight

Ears of field-grown wheat were harvested on 12 occasions between anthesis and ripeness. Fresh weight, dry weight, and total nitrogen content of selected grains were determined.

scholarly journals Impact of Mycorrhizal Fungal Inoculum on the Growth of Fenugreek in Different Soil Samples

2021 ◽  
Vol 12 (1) ◽  
pp. 12
Author(s):  
Sana Kanwal ◽  
Muhammad Rashid ◽  
Sana Ghafar ◽  
Komal Nawwal ◽  
Sana Ajmal ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Clay Soil ◽  
Dry Weight ◽  
Soil Samples ◽  
Symbiotic Association ◽  
Aromatic Plant ◽  
Fresh Weight ◽  
Trigonella Foenum Graecum ◽  
And Control ◽  
The Impact

Fenugreek (Trigonella foenum-graecum) is an aromatic plant that yields secondary metabolites, continuously used for the readiness of food and medicines. The current study is conducted to assess the impact of inoculation of mycorrhiza on the growth of Trigonella foenum-graecum in different soil samples. The current study presents two arrangements of treatment in experimental and control pots. Different soil samples were obtained from different areas for experimental and control pots. In experimental pots, mycorrhiza fungi inoculation is introduced. The six pots were filled with each soil sample. Out of the six pots, three were control pots, and three were inoculated pots. In each pot, 6 kg soil was filled. It was observed that there were more leaves in the experimental (inoculated) pots and fewer in the fenugreek (non-inoculated) pots. The fresh and dry weight of the shoots and roots was taken. However, it was examined that the fresh weight of the shoots and roots of the inoculated pots was more compared to the non-inoculated pots. But the difficulty is seen in the clay control pots of clay soil because of the compactness of the clay soil. It was concluded that fenugreek showed more growth in inoculated pots compared to non-inoculated pots. It was also concluded that mycorrhizal fungal showed symbiotic association with fenugreek plants.

scholarly journals Substrates with organic residues in Fritillaria cirrhosa seedlings for bulbus production

2019 ◽  
Vol 40 (3) ◽  
pp. 1079
Author(s):  
Ye Hu ◽  
Xuan Liu ◽  
Meng Ye
Keyword(s):  
Nitrogen Content ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Leaf Nitrogen ◽  
Leaf Length ◽  
Leaf Nitrogen Content ◽  
Organic Residues ◽  
Fresh Weight ◽  
Forestry Residues ◽  
Fritillaria Cirrhosa

China is abundant in organic residues from agricultural and forestry efforts. Bulbus Fritillaria cirrhosa D. Don (BFC) is a precious wild herb of Liliaceae that grows in the Tibetan area of China and the bulbs are used as a source of medicine. The shortage of germplasm resources restricts the development of traditional Chinese medicinal materials, so improved cultivation methods are urgently needed. The objective of this study was to study the effects of different substrates on the growth of F. cirrhosa seedlings. Sawdust and vermi-compost are common organic residues and were selected as nursery materials to supplement efforts to cultivate Fritillaria cirrhosa D. Don from ripe seeds and bulbs. The experiments were conducted in a plastic greenhouse in Ya’an of Sichuan Province from January to May 2013. Vegetative parameters of leaf length, leaf width, seedling fresh weight, seedling dry weight, and bulb size and fresh weight were measured. Additionally, the emergence ratio and preservation rate were calculated. The following results were obtained: (1) Sowing seeds of F. cirrhosa with 70% vermi-compost and 30% sawdust gave the best emergence rate, leaf length (60 days after seedling emergence, 60 DAS), and bulb dry weight (at harvest, 93 DAS). (2) The leaf nitrogen content (60 DAS) was positively correlated with the nitrogen content of bulbs (at harvest, 93 DAS). From the data, we concluded that forestry residues such as sawdust and vermi-compost can serve as excellent growing substrates of BFC seedlings.

scholarly journals Inflorescence Initiation in Lolium Temulentum L. IX. Some Chemical Changes in the Shoot Apex at Induction

1967 ◽  
Vol 20 (1) ◽  
pp. 1 ◽  
Author(s):  
AHGC Rijven ◽  
LT Evans
Keyword(s):  
Nitrogen Content ◽  
Shoot Apex ◽  
Phosphorus Content ◽  
Dry Weight ◽  
Cell Number ◽  
Fresh Weight ◽  
Residual Phosphorus ◽  
Residual Nitrogen ◽  
Long Day ◽  
Rna And Dna

Plants of L. temulentum were induced to flower by exposure to 1 long day. Shoot apices harvested from vegetative plants prior to the long�day exposure (day I) and others harvested on days IV and VI were analysed for length, fresh weight, total and residual (after extraction for solubles and lipids) dry weight, total and residual nitrogen content, residual phosphorus, and RNA and DNA phosphorus content. Cell number per apex was also established for two harvests.

scholarly journals Growth and Antioxidant Responses in Iron-Biofortified Lentil under Cadmium Stress

Toxics ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 182
Author(s):  
Ruchi Bansal ◽  
Swati Priya ◽  
Harsh Kumar Dikshit ◽  
Sherry Rachel Jacob ◽  
Mahesh Rao ◽  
...  
Keyword(s):  
Growth Parameters ◽  
Dry Weight ◽  
Cadmium Stress ◽  
Antioxidant Enzyme Activities ◽  
Limited Information ◽  
Cd Stress ◽  
Fresh Weight ◽  
Cd Uptake ◽  
Cd Tolerance ◽  
Controlled Conditions

Cadmium (Cd) is a hazardous heavy metal, toxic to our ecosystem even at low concentrations. Cd stress negatively affects plant growth and development by triggering oxidative stress. Limited information is available on the role of iron (Fe) in ameliorating Cd stress tolerance in legumes. This study assessed the effect of Cd stress in two lentil (Lens culinaris Medik.) varieties differing in seed Fe concentration (L4717 (Fe-biofortified) and JL3) under controlled conditions. Six biochemical traits, five growth parameters, and Cd uptake were recorded at the seedling stage (21 days after sowing) in the studied genotypes grown under controlled conditions at two levels (100 μM and 200 μM) of cadmium chloride (CdCl2). The studied traits revealed significant genotype, treatment, and genotype × treatment interactions. Cd-induced oxidative damage led to the accumulation of hydrogen peroxide (H2O2) and malondialdehyde in both genotypes. JL3 accumulated 77.1% more H2O2 and 75% more lipid peroxidation products than L4717 at the high Cd level. Antioxidant enzyme activities increased in response to Cd stress, with significant genotype, treatment, and genotype × treatment interactions (p < 0.01). L4717 had remarkably higher catalase (40.5%), peroxidase (43.9%), superoxide dismutase (31.7%), and glutathione reductase (47.3%) activities than JL3 under high Cd conditions. In addition, L4717 sustained better growth in terms of fresh weight and dry weight than JL3 under stress. JL3 exhibited high Cd uptake (14.87 mg g−1 fresh weight) compared to L4717 (7.32 mg g−1 fresh weight). The study concluded that the Fe-biofortified lentil genotype L4717 exhibited Cd tolerance by inciting an efficient antioxidative response to Cd toxicity. Further studies are required to elucidate the possibility of seed Fe content as a surrogacy trait for Cd tolerance.

scholarly journals Applications of plant growth promoting bacteria and Trichoderma spp. for controlling Orobanche crenata in faba bean

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Mahmoud Ahmed Touny El-Dabaa ◽  
Hassan Abd-El-Khair
Keyword(s):  
Plant Growth ◽  
Faba Bean ◽  
Growth Parameters ◽  
Dry Weight ◽  
Research Centre ◽  
Plant Growth Promoting Bacteria ◽  
Fresh Weight ◽  
Orobanche Crenata ◽  
Trichoderma Spp ◽  
Shoot Dry Weight

Abstract Background Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 108 propagules/ml and the bacterium inocula were adjusted at 107–109 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The bio-control agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.

scholarly journals Culture Dependent and Independent Analysis of Potential Probiotic Bacterial Genera and Species Present in the Phyllosphere of Raw Eaten Produce

2019 ◽  
Vol 20 (15) ◽  
pp. 3661 ◽  
Author(s):  
Sascha Patz ◽  
Katja Witzel ◽  
Ann-Christin Scherwinski ◽  
Silke Ruppel
Keyword(s):  
Protein Pattern ◽  
Probiotic Bacteria ◽  
Lepidium Sativum ◽  
Intact Protein ◽  
Bacillus Species ◽  
Thymus Vulgaris ◽  
Petroselinum Crispum ◽  
Bacterial Strains ◽  
Fresh Weight ◽  
Trigonella Foenum Graecum L

The plant phyllosphere is colonized by a complex ecosystem of microorganisms. Leaves of raw eaten vegetables and herbs are habitats for bacteria important not only to the host plant, but also to human health when ingested via meals. The aim of the current study was to determine the presence of putative probiotic bacteria in the phyllosphere of raw eaten produce. Quantification of bifidobacteria showed that leaves of Lepidium sativum L., Cichorium endivia L., and Thymus vulgaris L. harbor between 103 and 106 DNA copies per gram fresh weight. Total cultivable bacteria in the phyllosphere of those three plant species ranged from 105 to 108 CFU per gram fresh weight. Specific enrichment of probiotic lactic acid bacteria from C. endivia, T. vulgaris, Trigonella foenum-graecum L., Coriandrum sativum L., and Petroselinum crispum L. led to the isolation of 155 bacterial strains, which were identified as Pediococcus pentosaceus, Enterococcus faecium, and Bacillus species, based on their intact protein pattern. A comprehensive community analysis of the L. sativum leaves by PhyloChip hybridization revealed the presence of genera Bifidobacterium, Lactobacillus, and Streptococcus. Our results demonstrate that the phyllosphere of raw eaten produce has to be considered as a substantial source of probiotic bacteria and point to the development of vegetables and herbs with added probiotic value.

scholarly journals Effect of Organic Manures on Growth, Rhizome Yield and Quality Attributes of Turmeric (Curcuma longa L.)

2012 ◽  
Vol 10 (1) ◽  
pp. 16-22 ◽  
Author(s):  
M. Z. U. Kamal ◽  
M. N. Yousuf
Keyword(s):  
Leaf Area ◽  
Dry Matter ◽  
Curcuma Longa ◽  
Dry Weight ◽  
Fresh Weight ◽  
Area Index ◽  
Yield Attributes ◽  
Organic Manures ◽  
Neem Cake ◽  
Yield And Quality

The investigation was carried out to evaluate the effect of different organic manures on turmeric with reference to vegetative growth, biomass production, rhizome yield and its attributes of turmeric (Curcuma longa L.). Turmeric showed better response to the application of organic manures. Plant with neem cake application had the taller plant (79.30 cm), maximum number of tillers per plant (5.40), leaf number (5.40), leaf area (44.09) leaf area index (0.429), fresh weight of halum ( 190.05g), fresh weight of root (49.13 g), fresh weight of rhizome per plant (256.21 g) and dry weight of halum (15.21g), dry weight of root (7.32 g), dry weight of rhizome per plant (40.35 g), total dry matter yield (6.85 t ha-1) than those received other types of manures. Moreover, yield attributes such as number of mother rhizomes per plant-1 (1.75), more number of primary rhizomes per plant-1 (5.19), secondary rhizomes per plant-1 (18.03) and tertiary rhizomes per plant (7.69) were also highly accelerated by neem cake application. Similarly, the same treatment expressed the best in terms of size of mother rhizome (7.69 cm), primary rhizome (21.86 cm) and secondary rhizomes (7.05 cm).All these parameters in cumulative contributed to  produce the highest estimated fresh rhizomes yield & cured rhizomes yield (29.48 t ha-1, 5.59 t ha-1 respectively). The highest curing percentage (20.28) was observed in T3 treatment having mustard cake@ 2.0 t/ha. Thus, organic manure like neem cake was best fitted natural fertilizer for turmeric cultivation.DOI: http://dx.doi.org/10.3329/agric.v10i1.11060The Agriculturists 2012; 10(1): 16-22

scholarly journals Foliar applications of bio-fabricated selenium nanoparticles to improve the growth of wheat plants under drought stress

2020 ◽  
Vol 9 (1) ◽  
pp. 706-714
Author(s):  
Muhammad Ikram ◽  
Naveed Iqbal Raja ◽  
Bilal Javed ◽  
Zia-ur-Rehman Mashwani ◽  
Mubashir Hussain ◽  
...  
Keyword(s):  
Drought Stress ◽  
Field Capacity ◽  
Dry Weight ◽  
Positive Control ◽  
Morphological Characterization ◽  
Leaf Length ◽  
Selenium Nanoparticles ◽  
Fresh Weight ◽  
Wheat Varieties ◽  
Shoot Dry Weight

AbstractThe present study was aimed to biosynthesize selenium nanoparticles (SeNPs) and assess their foliar applications to improve the growth of wheat plants under controlled irrigation and drought stress. Bud aqueous extract of Allium sativum L. was used as a reducing and stabilizing agent of SeNPs followed by their optical and morphological characterization by using ultraviolet-visible spectroscopy, scanning electron microscopy, Fourier-transform infrared, and energy dispersive X-ray analysis. Various concentrations of SeNPs (10, 20, 30, and 40 mg/L) were applied exogenously to drought-tolerant (V1) and drought-susceptible (V2) wheat varieties at the trifoliate stage. Under the positive control conditions, plants were irrigated with 450 mL of water/pot (100% field capacity); and under water-deficit environment, plants were irrigated with 160 mL of water/pot (35% field capacity). Remarkable increase in plant height, shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, leaf area, leaf number, and leaf length has been observed when 30 mg/L concentration of SeNPs was used. However, the plant morphological parameters decreased gradually at higher concentrations (40 mg/L) in both selected wheat varieties. Therefore, 30 mg/L concentration of SeNPs was found most preferable to enhance the growth of selected wheat varieties under normal and water-deficient conditions.

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