scholarly journals Molecular detection and characterization of phytoplasma associated with China aster (Callistephus chinensis) phyllody in India

2017 ◽  
Vol 9 (3) ◽  
pp. 1831-1834
Author(s):  
Shweta Kumari ◽  
H. A. Prameela ◽  
Manjunath Manjunath ◽  
S. Hurakadli ◽  
K. T. Rangaswamy
Keyword(s):  
Flowering Plant ◽  
Economic Losses ◽  
Universal Primer ◽  
Phytoplasma Strain ◽  
Pcr Product ◽  
Plant Grown ◽  
China Aster ◽  
Relationship Of ◽  
Maximum Sequence

China aster (Callistephus chinensis L.) is one of the most popular annual flowering plant grown through-out the world. Phyllody disease of China aster is a phytoplasma associated disease that induces severe economic losses. Phytoplasmal disease in China aster was assessed for phytoplasma by direct polymerase chain reaction primed by using phytoplasma universal primer pairs PI/P7. A 1.8 Kb DNA fragments encoding the portion of phyto-plasma 16SrDNA amplified by PCR was cloned and sequenced. Sequencing of the PCR product and BLAST analy-sis indicated that China aster phyllody phytoplasma strain shared maximum sequence identity (99%) with strains of Peanut Witches’ broom (16SrII) phytoplasma group. Phylogenetic relationship of 16SrDNA sequence of China aster phyllody phytoplasma strain in the present study confirmed association of Peanut Witches’ broom (16SrII) group of phytoplasmas with China aster phyllody disease in India.

Skeletal elements of crinoid echinoderms: High-resolution structural and microanalytical results

1983 ◽  
Vol 41 ◽  
pp. 194-195
Author(s):  
D. F. Blake ◽  
L. F. Allard ◽  
D. R. Peacor
Keyword(s):  
High Resolution ◽  
Marine Invertebrates ◽  
Sea Urchins ◽  
Structural Features ◽  
Sea Stars ◽  
High Resolution Tem ◽  
Relationship Of ◽  
The Relationship ◽  
Insight Into

Echinodermata is a phylum of marine invertebrates which has been extant since Cambrian time (c.a. 500 m.y. before the present). Modern examples of echinoderms include sea urchins, sea stars, and sea lilies (crinoids). The endoskeletons of echinoderms are composed of plates or ossicles (Fig. 1) which are with few exceptions, porous, single crystals of high-magnesian calcite. Despite their single crystal nature, fracture surfaces do not exhibit the near-perfect {10.4} cleavage characteristic of inorganic calcite. This paradoxical mix of biogenic and inorganic features has prompted much recent work on echinoderm skeletal crystallography. Furthermore, fossil echinoderm hard parts comprise a volumetrically significant portion of some marine limestones sequences. The ultrastructural and microchemical characterization of modern skeletal material should lend insight into: 1). The nature of the biogenic processes involved, for example, the relationship of Mg heterogeneity to morphological and structural features in modern echinoderm material, and 2). The nature of the diagenetic changes undergone by their ancient, fossilized counterparts. In this study, high resolution TEM (HRTEM), high voltage TEM (HVTEM), and STEM microanalysis are used to characterize tha ultrastructural and microchemical composition of skeletal elements of the modern crinoid Neocrinus blakei.

scholarly journals On cryptographic properties of (n + 1)-bit S-boxes constructed by known n-bit S-boxes

2020 ◽  
Vol 15 (1) ◽  
pp. 258-265
Author(s):  
Yu Zhou ◽  
Daoguang Mu ◽  
Xinfeng Dong
Keyword(s):  
Sufficient Conditions ◽  
Sum Of Squares ◽  
Basic Component ◽  
Necessary And Sufficient Conditions ◽  
Autocorrelation Functions ◽  
Walsh Spectrum ◽  
Cryptographic Algorithms ◽  
Necessary And Sufficient ◽  
Relationship Of

AbstractS-box is the basic component of symmetric cryptographic algorithms, and its cryptographic properties play a key role in security of the algorithms. In this paper we give the distributions of Walsh spectrum and the distributions of autocorrelation functions for (n + 1)-bit S-boxes in [12]. We obtain the nonlinearity of (n + 1)-bit S-boxes, and one necessary and sufficient conditions of (n + 1)-bit S-boxes satisfying m-order resilient. Meanwhile, we also give one characterization of (n + 1)-bit S-boxes satisfying t-order propagation criterion. Finally, we give one relationship of the sum-of-squares indicators between an n-bit S-box S0 and the (n + 1)-bit S-box S (which is constructed by S0).

Molecular detection of equine infectious anemia virus in clinically normal, seronegative horses in an endemic area of Mexico

2021 ◽  
pp. 104063872110061
Author(s):  
César I. Romo-Sáenz ◽  
Patricia Tamez-Guerra ◽  
Aymee Olivas-Holguin ◽  
Yareellys Ramos-Zayas ◽  
Nelson Obregón-Macías ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Equine Infectious Anemia Virus ◽  
Antibody Detection ◽  
Economic Losses ◽  
Clinically Normal ◽  
Pcr Product ◽  
Surveillance Programs ◽  
Equine Infectious Anemia ◽  
Anemia Virus ◽  
Equine Industry

Equine infectious anemia (EIA) is a highly infectious disease in members of the Equidae family, caused by equine infectious anemia virus (EIAV). The disease severity ranges from subclinical to acute or chronic, and causes significant economic losses in the equine industry worldwide. Serologic tests for detection of EIAV infection have some concerns given the prolonged seroconversion time. Therefore, molecular methods are needed to improve surveillance programs for this disease. We attempted detection of EIAV in 6 clinical and 42 non-clinical horses in Nuevo Leon State, Mexico, using the agar gel immunodiffusion (AGID) test for antibody detection, and nested and hemi-nested PCR for detection of proviral DNA. We found that 6 of 6, 5 of 6, and 6 of 6 clinical horses were positive by AGID, nested PCR, and hemi-nested PCR, respectively, whereas 0 of 42, 1 of 42, and 9 of 42 non-clinical horses were positive by these tests, respectively. BLAST analysis of the 203-bp 5′-LTR/ tat segment of PCR product revealed 83–93% identity with EIAV isolates in GenBank and reference strains from other countries. By phylogenetic analysis, our Mexican samples were grouped in a different clade than other sequences reported worldwide, indicating that the LRT/ tat region represents an important target for the detection of non-clinical horses.

scholarly journals Molecular and Pathogenicity Characterization of Sphaceloma manihoticola Isolates from South-Central Brazil

Plant Disease ◽  
2003 ◽  
Vol 87 (11) ◽  
pp. 1322-1328 ◽  
Author(s):  
Elizabeth Alvarez ◽  
Juan Fernando Mejia ◽  
Teresa L. Valle
Keyword(s):  
Morphological Characteristics ◽  
Restriction Pattern ◽  
Poly Merase Chain Reaction ◽  
Highly Pathogenic ◽  
Central Brazil ◽  
Host Interaction ◽  
South Central ◽  
Pcr Product ◽  
Pathogenic Variation

Isolates of Sphaceloma manihoticola, the asexual stage of Elsinoe brasiliensis, were collected from several regions of south-central Brazil. The isolates were obtained from samples of leaves, stems, and petioles of cassava (Manihot esculenta) and the weedy Euphorbia heterophylla (“amendoim bravo”) by directly plating infected tissue onto acidified potato dextrose agar. For pathogenicity studies, 19 isolates were inoculated onto each of two cassava cultivars, MBRA 703 as a susceptible cultivar and MBRA 12 as a resistant cultivar to S. manihoticola. MBRA 703, with the greatest pathogenicity to 58% (11) of the isolates, showed an intermediate pathogenic reaction to 16% (3) of the isolates, and was less pathogenic to 26% (5) of the isolates. MBRA 12, with a less pathogenic reaction to 63% (12) of the isolates, showed an intermediate pathogenic reaction to 16% (3) of the isolates, and was highly pathogenic to 21% (4) of the isolates. The isolates were verified as belonging to the genus Sphaceloma based on their morphological characteristics, including conidia and hyphae of monoconidial isolate. Conidia of isolates were small, thin-walled, ellipsoid to (rarely) globose, commonly with one or two gut-tules. Conidiophores were phialides, hyaline to slightly pigmented 0-to-1 septate; conidiophores from the weedy specie were phialides, hyaline to brown 0-to-2 septate producing hyaline conidia. The isolates also were verified as belonging to the genus Sphaceloma by using a poly-merase chain reaction (PCR) assay, which detected a 645-bp band in all isolates except two (1 and 6) for which the PCR product had 600 bp. Digestion of the amplified product with the enzymes MspI and CfoI allowed differences to be detected in restriction patterns among isolates. A homogeneous banding pattern was obtained for 17 of the isolates but a different restriction pattern was obtained for isolates 1 and 6 of E. heterophylla. This suggests the possibility of another species within this group of isolates. The results indicate the presence of pathogenic variation among isolates of the fungus and an isolate-host interaction, because statistically significant differences were observed between the two cassava cultivars in response to inoculation with the isolates of S. manihoticola.

scholarly journals Molecular Typing ofNeisseria gonorrhoeaeIsolates by Opa-Typing and Ribotyping in New Delhi, India

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
Pejvak Khaki ◽  
Preena Bhalla ◽  
Ahmad Mir Fayaz ◽  
Sohiela Moradi Bidhendi ◽  
Majid Esmailzadeh ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Preventive Measures ◽  
Restriction Enzymes ◽  
New Delhi ◽  
Pcr Product ◽  
Sexual Contacts ◽  
High Level ◽  
Epidemiological Characteristics ◽  
Epidemiological Characterization

Control and preventive measures for gonococcal infections are based on precise epidemiological characteristics ofN. gonorrhoeaeisolates. In the present study the potential utility of opa-typing and ribotyping for molecular epidemiological study of consecutive gonococcal strains was determined. Sixty gonococcal isolates were subjected to ribotyping with two restriction enzymes,AvaII andHincII, and opa-typing withTaqI andHpaII for epidemiological characterization of gonococcal population. Ribotyping withAvaII yielded 6 ribotype patterns while twelve RFLP patterns were observed withHincII. Opa-typing of the 60 isolates revealed a total 54 opa-types, which 48 were unique and 6 formed clusters. Fifty-two opa-types were observed withTaqI-digested PCR product while opa-typing withHpaII demonstrated 54 opa-types. The opa-types from isolates that were epidemiologically unrelated were distinct, whereas those from the sexual contacts were identical. The results showed that opa-typing is highly useful for characterizing gonococcal strains from sexual contacts and has more discriminatory than ribotyping that could differentiate between gonococci of the same ribotype. The technique even with a single restriction enzyme has a high level of discrimination (99.9%) between epidemiologically unrelated isolates. In conclusion, the molecular methods such as opa-typing and ribotyping can be used for epidemiological characterization of gonococcal strains.

scholarly journals Autofluorescence spectroscopy and imaging: a tool for biomedical research and diagnosis

2014 ◽  
Author(s):  
A.C. Croce ◽  
G. Bottiroli
Keyword(s):  
Scientific Community ◽  
Data Interpretation ◽  
Near Ir ◽  
Endogenous Fluorophores ◽  
Biological Substrate ◽  
Autofluorescence Spectroscopy ◽  
Uv Visible ◽  
Biological Substrates ◽  
Relationship Of

Native fluorescence, or autofluorescence (AF), consists in the emission of light in the UV-visible, near-IR spectral range when biological substrates are excited with light at suitable wavelength. This is a well-known phenomenon, and the strict relationship of many endogenous fluorophores with morphofunctional properties of the living systems, influencing their AF emission features, offers an extremely powerful resource for directly monitoring the biological substrate condition. Starting from the last century, the technological progresses in microscopy and spectrofluorometry were convoying attention of the scientific community to this phenomenon. In the future, the interest in the autofluorescence will certainly continue. Current instrumentation and analytical procedures will likely be overcome by the unceasing progress in new devices for AF detection and data interpretation, while a progress is expected in the search and characterization of endogenous fluorophores and their roles as intrinsic biomarkers.

scholarly journals Hubungan Kekerabatan Sapi Aceh dengan Menggunakan Daerah Displacement-loop

2008 ◽  
Vol 8 (2) ◽  
pp. 9-14 ◽  
Author(s):  
Mohd. Agus Nashri Abdullah
Keyword(s):  
Whole Blood ◽  
Bos Indicus ◽  
Loop Analysis ◽  
Blood Samples ◽  
Bos Javanicus ◽  
Pcr Product ◽  
Two Samples ◽  
D Loop ◽  
Whole Blood Samples ◽  
Relationship Of

Relationship of aceh cattle using displacement-loop regionABSTRACT. The aims of this study were to describe relationship of D-loop of mtDNA Aceh cattle which is useful database for conducting conservation programme. The whole blood samples were collected (8 samples for D-loop analysis) from four locations which were Aceh Besar, Pidie, North Aceh regencies and Banda Aceh city. Out group whole blood samples were collected from two samples from Bali cattles (Bali Island), Madura cattle (Madura Island), Pesisir cattle (West Sumatera) respectively and one sample from PO cattle (West Java). Amplification of D-loop sequences of mtDNA with BIDLF and BIDLR primary have PCR product 980 bp. The Data were analyzed using Squint 1.02 and MEGA 4.0 programme. Result of analysis indicate that Aceh cattle have nearer relationship with zebu and there is items inset of genetik Bali cattle (Bos javanicus) at the end sequences start ke-354 situs up to 483, so that the origin Aceh cattle was from Bos indicus which have hybridization with Bos javanicus.

scholarly journals Molecular characterization of porcine epidemic diarrhoea virus (PEDV) in Poland reveals the presence of swine enteric coronavirus (SeCoV) sequence in S gene

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258318
Author(s):  
Marta Antas ◽  
Monika Olech ◽  
Anna Szczotka-Bochniarz
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Rna Virus ◽  
Economic Losses ◽  
High Morbidity ◽  
Antigenic Analysis ◽  
S Gene ◽  
Porcine Epidemic Diarrhoea Virus ◽  
Diarrhoea Virus

Porcine epidemic diarrhoea (PED) is a highly contagious enteric viral disease of pigs with a high morbidity and mortality rate, which ultimately results in huge economic losses in the pig production sector. The etiological agent of this disease is the porcine epidemic diarrhoea virus (PEDV) which is an enveloped, positive single-stranded RNA virus. The aim of this study was to perform molecular characterization of PEDV to identify the strains circulating in Poland. In this study, 662 faecal samples from 2015 to 2021 were tested with reverse transcription quantitative real-time PCR (RT-qPCR) and the results showed that 3.8% of the tested samples revealed a positive result for PEDV. A phylogenetic analysis of the complete genome and complete S gene sequences showed that Polish PEDV strains belonged to the G1b (S-INDEL) subgroup and were closely related to the European PEDV strains isolated from 2014 to 2019. Furthermore, RDP4 analysis revealed that the Polish PEDV strains harboured a recombinant fragment of ~400 nt in the 5’ end of S gene with PEDV and swine enteric coronavirus (SeCoV) being the major and minor parents, respectively. Antigenic analysis showed that the aa sequences of neutralizing epitopes were conserved among the Polish PEDV strains. Only one strain, #0100/5P, had a unique substitution in the COE epitope. However, Polish PEDV strains showed several substitutions, especially in the COE antigen, as compared to the classical strain CV777. To the best of our knowledge, this is the first report concerning the molecular characterization of porcine epidemic diarrhoea virus strains, as well as the first phylogenetic analysis for PEDV in Poland.

scholarly journals Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae

2017 ◽  
Author(s):  
Olga Gavrilova ◽  
Anna Skritnika ◽  
Tatiana Gagkaeva
Keyword(s):  
Intraspecific Diversity ◽  
Northern Europe ◽  
Growth Defect ◽  
Organic Media ◽  
Auxotrophic Mutants ◽  
Pcr Product ◽  
Subgroup Ii ◽  
Free Media ◽  
Identification And Characterization

Analysis of 49 strains of F. langsethiae originating from northern Europe (Russia, Finland, Sweden, UK, Norway, and Latvia) revealed the presence of spontaneous auxotrophic mutants that reflect natural intraspecific diversity. Our investigations detected that 49.0% of F. langsethiae strains were auxotrophic mutants for biotin, and 8.2% of the strains required thiamine as a growth factor. They failed to grow on vitamin-free media. For both prototrophic and auxotrophic strains, no growth defect was observed in rich organic media. Without essential vitamins, a significant reduction in the growth of the auxotrophic strains results in a decrease of the formation of T-2 toxin and diacetoxyscirpenol. In addition, all analysed F. langsethiae strains were distinguished into two subgroups based on PCR product sizes. According to our results, 26 and 23 strains of F. langsethiae belong to subgroups I and II respectively. We determined that the deletion in the IGS region of the rDNA of F. langsethiae belonging to subgroup II is linked with temperature sensitivity and causes a decrease in strain growth at 30 °C. Four thiamine auxotrophic strains were found in subgroup I, while 21 biotin auxotrophic strains were detected in subgroups II. To the best of our knowledge, the spontaneous mutations in F. langsethiae observed in the present work have not been previously reported.

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