Detection of the Active Compounds of the Hot Aqueous Extract of Cinnamomum cassia using FTIR Technology and Testing as Anti-bacterial and Antioxidant

2021 ◽  
Vol 8 (6) ◽  
pp. 95-101
Author(s):  
Afnan I. Abdulwahab
Keyword(s):  
Staphylococcus Aureus ◽  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Bacillus Sp ◽  
Ftir Analysis ◽  
Bacterial Strains ◽  
Active Compounds ◽  
Cinnamon Bark ◽  
Cinnamomum Cassia ◽  
Inhibitory Ability

The results of the FTIR analysis of the hot aqueous extract of bark indicated that it contains many groups and active compounds, and the results of the bacterial tests conducted by digging and spreading method showed a high inhibitory activity of the hot aqueous extract of cinnamon against all pathogenic bacterial strains and at different concentrations. It was observed that the highest efficacy of the extract was against bacteria an inhibition diameter of 35 mm at concentration 800 mg/mL in Bacillus sp. while the bacterial strains were resistant to most of the antibiotics, in the study (Amoxicillin, Gentamycin, Ampicillin, Erythromycin, Tetracycline) except for gentamicin, which showed inhibition of bacteria Staphylococcus aureus with a diameter 19mm and Serratia sp. in diameter17mm. We conclude from the study that the cinnamon plant contains many active compounds and that the hot aqueous extract of Cinnamomum cassia (cinnamon) bark has a high inhibitory ability for different bacterial strains, which exceeded the inhibitory ability of antibiotics.

The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Hussein A Kadhum ◽  
Thualfakar H Hasan2
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Growth ◽  
Broad Spectrum ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
Inhibitory Ability ◽  
Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

scholarly journals Inhibition of Staphylococcus aureus LC 554891 by Moringa oleifera Seed Extract either Singly or in Combination with Antibiotics

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4583
Author(s):  
Gamal Enan ◽  
Abdul-Raouf Al-Mohammadi ◽  
Samir Mahgoub ◽  
Seham Abdel-Shafi ◽  
Eman Askar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oils ◽  
Inhibitory Activity ◽  
Moringa Oleifera ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Resistance Index ◽  
Aureus Strain ◽  
Rrna Gene ◽  
Bacterial Strains

Bacterial outbreaks caused by Staphylococcus aureus (S. aureus) are interesting due to the existence of multidrug resistant (MDR) isolates. Therefore, there is a need to develop novel ways to control such MDR S. aureus. In this study, some natural agents such as honey bee (HB), extracts of either Moringa oleifera seeds (MSE), or leaves (MLE) and essential oils of garlic, clove, and moringa were studied for their inhibitory activity against this S. aureus pathogen. About 100 food samples including beef luncheon (n = 25), potato chips (n = 50), and corn flakes (n = 25) were investigated for possible pollution with the S. aureus bacteria. The isolated bacteria suspected to belong S. aureus that grew well onto Baird–Parker agar (Oxoid) and shiny halo zones and positive coagulase reaction were selected and identified by API-Kits; all of them that were approved belong to S. aureus (18 strains). The sensitivity of the obtained 18 S. aureus bacterial strains to 12 antibiotics were evaluated; all of them were resistant to ofloxacin; however, other antibiotics tested showed variable results. Interestingly, the S. aureus No. B3 isolated from beef luncheon was resistant to 10 antibiotics out of 12 ones tested. Multiple antibiotic resistance index (MAR) of this S. aureus strain was about 83.3%. Therefore, its identification was confirmed by sequencing of a 16S rRNA gene which approved a successful biochemical identification carried out by API Kits and such strain was designated S. aureus LC 554891. The genome of such strain appeared to contain mecA gene encoding methicillin resistance; it was found to contain hla, hlb, tsst-1, and finbA that encode α-blood hemolysis, β-blood hemolysis, toxic shock syndrome gene, and fibrinogen-binding protein gene, respectively. In addition, the virulence factors viz. sea; seb; sec encoding enterotoxins were detected in the DNA extracted from S. aureus B3 strain. Aqueous extract of Moringa oleifera seeds (MSE) showed inhibitory activity against S. aureus LC 554891 better than that obtained by tetracycline, essential oils or HB. Minimum inhibitory concentration (MIC) of MSE was 20µg/mL. Instrumental analysis of MSE showed 14 bioactive chemical compounds. Combinations of both MSE and tetracycline showed distinctive inhibitory activity against S. aureus LC 554891 than that obtained by either tetracycline or MSE singly.

scholarly journals Antibacterial Activity and Molecular Docking Studies of a Selected Series of Hydroxy-3-arylcoumarins

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2815 ◽  
Author(s):  
Pisano ◽  
Kumar ◽  
Medda ◽  
Gatto ◽  
Pal ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Molecular Docking ◽  
Structural Features ◽  
Docking Studies ◽  
Trna Synthetase ◽  
Hydroxyl Groups ◽  
Bacterial Strains ◽  
Active Compounds ◽  
Molecular Docking Studies

Antibiotic resistance is one of the main public health concerns of this century. This resistance is also associated with oxidative stress, which could contribute to the selection of resistant bacterial strains. Bearing this in mind, and considering that flavonoid compounds are well known for displaying both activities, we investigated a series of hydroxy-3-arylcoumarins with structural features of flavonoids for their antibacterial activity against different bacterial strains. Active compounds showed selectivity against the studied Gram-positive bacteria compared to Gram-negative bacteria. 5,7-Dihydroxy-3-phenylcoumarin (compound 8) displayed the best antibacterial activity against Staphylococcus aureus and Bacillus cereus with minimum inhibitory concentrations (MICs) of 11 g/mL, followed by Staphylococcus aureus (MRSA strain) and Listeria monocytogenes with MICs of 22 and 44 g/mL, respectively. Moreover, molecular docking studies performed on the most active compounds against Staphylococcus aureus tyrosyl-tRNA synthetase and topoisomerase II DNA gyrase revealed the potential binding mode of the ligands to the site of the appropriate targets. Preliminary structure–activity relationship studies showed that the antibacterial activity can be modulated by the presence of the 3-phenyl ring and by the position of the hydroxyl groups at the coumarin scaffold.

scholarly journals Natural Antibrowning Agents against Polyphenol Oxidase Activity in Annona muricata and Musa acuminata

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Michelle B. S. Weerawardana ◽  
Gobika Thiripuranathar ◽  
Priyani A. Paranagama
Keyword(s):  
Polyphenol Oxidase ◽  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Fruits And Vegetables ◽  
Musa Acuminata ◽  
Annona Muricata ◽  
Retail Markets ◽  
Cinnamon Bark ◽  
Oxidase Enzyme ◽  
Nutritional Benefits

Fresh-cut fruits and vegetables emerge as popular food for consumers in retail markets. However, a loss of millions of dollars yearly to the food industry has been due to discoloration of fruits and vegetables caused by a pronounced reaction called enzymatic browning, which is caused by the activity of the polyphenol oxidase enzyme present in most of the fruits and vegetables. The main objective of this study was to investigate the natural antibrowning effects of the aqueous extract of ginger and essential oil of cinnamon bark on PPO enzymatic activity in Annona muricata (katu anoda) and Musa acuminata (ash plantains), which are considered to be widely consumable by Sri Lankans due to its respective health benefits. The antibrowning activity analyzed using a UV-visible spectrophotometer at a wavelength of 525 nm showed that cinnamon bark oil of 0.0035 g/mL had a % inhibitory activity of 51.97 percent on PPO activity in Annona muricata and 49.51 percent on PPO activity in Musa acuminata, while the aqueous extract of ginger of 0.091 g/mL had a % inhibitory activity of 60.90 percent on PPO activity in Annona muricata and 48.10 percent on PPO activity in Musa acuminata, respectively. This study shows that cinnamon bark oil and ginger can be used as effective, natural, nontoxic antibrowning agents that can inhibit the activity of the PPO enzyme, thereby preventing the essence and nutritional benefits of fruits and vegetables.

scholarly journals Green synthesis of copper oxide nanoparticles using Bougainvillea leaves aqueous extract and antibacterial activity evaluation

2021 ◽  
Author(s):  
CI Chemistry International
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Copper Oxide ◽  
Green Synthesis ◽  
Aqueous Extract ◽  
Low Cost ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Bacterial Strains ◽  
Cost Efficient

The aim of present study is based on low cost, efficient, non-toxic and eco-friendly method for green synthesis of copper oxide nanoparticles (CuONPs) using Bougainvillea leaves aqueous extract. The green synthesized nanoparticles were subjected to characterization techniques UV–visible spectroscopy (UV–vis), X-ray diffraction (XRD), Fourier transform Infrared spectroscopy (FT-IR) and transmission electron microscope (TEM). The synthesized CuONPs were pure, predominantly spherical with sizes ranges from 8-20 nm. CuONPs showed excellent antimicrobial activity against various bacterial strains Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus. Moreover, E. coli and E. faecalis exhibited the highest sensitivity to CuONPs while Staphylococcus aureus was the least sensitive. Possible mechanisms of antimicrobial activity of CuONPs should be further investigated.

scholarly journals Antibacterial Activity of Lyophilized Aqueous Extract of Coriaria Myrtifolia from Northern Morocco

2013 ◽  
Vol 3 (3) ◽  
pp. 280-285
Author(s):  
Karima Hezbri ◽  
Maha Hafse ◽  
Abdellah Farah ◽  
Imed Sbissi ◽  
Maher Gtari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Aqueous Extract ◽  
The Other ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Streptomyces Sp ◽  
E Coli ◽  
Other Hand

This work aims to evaluate the antibacterial activity of lyophilized aqueous extract of Coriaria myrtifolia against twentythree bacterial strains belonging to twelve main genera, known to be involved in some pathologies and / or in the foodspoilage process: Bacillus sp., E. coli, E. hafnia, Enterococcus sp., Klebsiella sp,. Listeria monocytogenes, Pseudomonassp., Salmonella sp., Serratia sp., Shigella sp., S. aureus and Streptomyces sp. To achieve this goal, the disk diffusionmethod was used.The study revealed that C. myrtifolia’s aqueous extract is active against most of the tested strains and that activity isproportional to its concentration. The intensity of inhibition depends on the extract’s concentration on one hand and on thebacterial species tested on the other hand.

scholarly journals Study the effect of aqueous extracts of Pimpinella anisum , Foeniculum vulgara and agroup of different medical herbs on growth of bacteria ( Staphylococcus aureus) isolated from Sputum of bronchiolitis patients .

2008 ◽  
Vol 2 (1) ◽  
pp. 57-64
Author(s):  
Ibtihal E . AI – Ani ◽  
Sabah A . R . AI – Obaidi ◽  
Amna N . Jasim
Keyword(s):  
Staphylococcus Aureus ◽  
Aqueous Extract ◽  
Bacterial Growth ◽  
Nigella Sativa ◽  
Gum Arabic ◽  
Inhibitory Effect ◽  
Aqueous Extracts ◽  
Inhibitory Ability ◽  
Pimpinella Anisum ◽  
Growth Of Bacteria

This study was conducted to determine the inhibitory dose of the hot and cold aqueous extracts of Pimpinella anisum (PA) , Foeniculum vulgara (FV) , and agroup of different medical herbs on growth of bacteria , Staphylococcus aureus isolated from the sputum of bronchiolitis patients . Minimum Inhibitory concentration (MIC) method was used to test the sensitivity of the bacteria toward the above extract . The hot aqueous extract of (PA)in concentration of 23.8 and 28mg/ ml gave a strong inhibitory ability on the growth of the bacteria at MIC 0.01 mg/ ml compared with the highest concentration of 47.6 mg/ml which did not inhibit the bacterial growth at MIC 0.02 mg/ml . The cold aqueous extract of PA at 125 mg /ml concentration also did not inhibit the bacterial growth up to MIC 0.12 mg / ml . This study also revealed that a concentration of 17.12 mg / ml of hot aqueous extract of FV seeds had no inhibitory effect on growth up to MIC 0.008 mg / ml . The combined hot aqueous extract of PA and FV seeds in a ratio of 1gm to 0.5 gm respectively an 1gm to 1 gm (24and 40.32mg / ml respectively ) also did not have any inhibitory effect of bacterial growth up to MIC 0.002 and 0.004 mg / ml, respectively . The results of this experiment also revealed that hot aqueous extract of similar quantities and concentrations ( 17.28 and 31.74 mg /ml ) of a mixture of several medical herbs like ( PA , FV , Anthemis nobilis , Thymus bovei ,Gum Arabic, Nigella sativa , Althaea , and Cydonia vulgaris ) did not show any inhibitory action on bacterial growth up to MIC of 0.004 and 0.007 mg / ml . It could be concluded that the inhibitory ability of the hot aqueous extract of PA seeds is the best compared to the other extracts used . The inhibition of bacterial growth may need a carefully selected medical herbs in different concentrations and combination .

scholarly journals Antimicrobial Activities of Teucrium creticum against Reference Microbial Strains and Multi-drug Resistant Bacteria Isolated at an Oncology Ward

2019 ◽  
pp. 1-7
Author(s):  
Motasem Al-Masri ◽  
Ghadeer Omar ◽  
Adham Taha ◽  
Amira Abu Alsoud ◽  
Eman Tawafsha ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Aqueous Extract ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Resistant Bacteria ◽  
Dilution Method ◽  
Bacterial Strains ◽  
Patients With Cancer ◽  
Oncology Ward ◽  
National University

Aim: To determine antimicrobial activity of Teucrium creticum (T. creticum) leaves extract against bacterial and fungal reference strains and multidrug resistant bacteria isolated at an oncology ward. Study Design: Cross-sectional study. Place and Duration of Study: The study was carried out in department of biology and biotechnology in An-Najah National University in cooperation with the laboratory of the hospital of the university.  An-Najah National University is located in West Bank in Palestine. The research was performed from 8th of February to the 15th of April 2017.  Methodology: Teucrium creticum plant leaves were collected in Palestine, from which aqueous and methanolic extracts were prepared. Antimicrobial activities of T. creticum extracts were determined against reference bacterial and fungal strains as well as against 8 multidrug resistant bacteria isolated at an oncology ward.  Antibacterial and anti-yeast activities were determined by Micro broth dilution method, while anti-mold activities were determined by agar dilution method. Teucrium creticum methanolic extract strongly inhibited the growth of the studied reference bacterial strains, which were Staphylococcus aureus (MIC= 1.56 mg/ml) and Shigella sonnie (MIC=3.125 mg/ml).  In addition, most of the 8 multi-drug resistant bacterial strains isolated from patients with cancer (Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia Coli and Enterobacter cloacae) were also highly susceptible to methanolic extract (MIC=3.125 mg/ml).  Both the Staphylococcus aureus and Shigella sonnie reference strains were inhibited at lower level by the aqueous extract (MIC=12.5 mg/ml). All the bacterial strains isolated from patients with cancer were susceptible to aqueous extract at different levels (3.125 – 25 mg/ml).  Epidermophyton floccosum mold and Candida albicans yeast were strongly inhibited by aqueous extract, where the MIC values were 1.56 and 3.125 mg/ml, respectively.   Conclusion: T. creticum plant extracts showed promising antimicrobial activities against multidrug resistant bacterial isolates as well as against reference bacterial and fungal strains.

Lipids and Free Fatty Acids of Red Sea Avrainvillea amadelpha, Holothuria atra, and Sarcocornia fruticosa Inhibit Marine Bacterial Biofilms

2020 ◽  
Vol 17 (6) ◽  
pp. 466-471
Author(s):  
Usama W. Hawas ◽  
Fekri Shaher ◽  
Mohamed Ghandourah ◽  
Lamia T. Abou El-Kassem ◽  
Sathianeson Satheesh ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Red Sea ◽  
Inhibitory Activity ◽  
Sea Cucumber ◽  
Bacterial Biofilms ◽  
Antibiofilm Activity ◽  
Bacterial Strains ◽  
Holothuria Atra ◽  
Sarcocornia Fruticosa

This study aimed at evaluating the antibiofilm activity of the Red Sea metabolites from green alga Avrainvillea amadelpha, sea cucumber Holothuria atra and costal plant Sarcocornia fruticosa against three biofilm bacterial strains isolated from Jeddah coast. Free fatty acids (FFAs) and other lipoidal matters were extracted from these organisms and analyzed by GC-MS. The composition of lipoidal fractions showed that A. amadelpha is rich by 74% saturated FAs, while sea cucumber H. atra revealed high content (60%) of unsaturated FAs. Palmitic acid is the major FA component in all species ranging from 14.5 to 26.7%. Phytol, sterols and hydrocarbons (C8-C29) were represented in the alga A. amadelpha as high contents with values 25.8, 21.9 and 18.5%, respectively. The extracts and lipoidal contents showed biofilm inhibitory activity against the isolated bacterial strains, where the unsaponified lipoidal fraction of S. fruticosa exhibited highest inhibitory activity against Planomicrobium sp. at concentration of 200 µg/mL.

Sign in / Sign up

Export Citation Format

Share Document