scholarly journals Study the effect of aqueous extracts of Pimpinella anisum , Foeniculum vulgara and agroup of different medical herbs on growth of bacteria ( Staphylococcus aureus) isolated from Sputum of bronchiolitis patients .

2008 ◽  
Vol 2 (1) ◽  
pp. 57-64
Author(s):  
Ibtihal E . AI – Ani ◽  
Sabah A . R . AI – Obaidi ◽  
Amna N . Jasim
Keyword(s):  
Staphylococcus Aureus ◽  
Aqueous Extract ◽  
Bacterial Growth ◽  
Nigella Sativa ◽  
Gum Arabic ◽  
Inhibitory Effect ◽  
Aqueous Extracts ◽  
Inhibitory Ability ◽  
Pimpinella Anisum ◽  
Growth Of Bacteria

This study was conducted to determine the inhibitory dose of the hot and cold aqueous extracts of Pimpinella anisum (PA) , Foeniculum vulgara (FV) , and agroup of different medical herbs on growth of bacteria , Staphylococcus aureus isolated from the sputum of bronchiolitis patients . Minimum Inhibitory concentration (MIC) method was used to test the sensitivity of the bacteria toward the above extract . The hot aqueous extract of (PA)in concentration of 23.8 and 28mg/ ml gave a strong inhibitory ability on the growth of the bacteria at MIC 0.01 mg/ ml compared with the highest concentration of 47.6 mg/ml which did not inhibit the bacterial growth at MIC 0.02 mg/ml . The cold aqueous extract of PA at 125 mg /ml concentration also did not inhibit the bacterial growth up to MIC 0.12 mg / ml . This study also revealed that a concentration of 17.12 mg / ml of hot aqueous extract of FV seeds had no inhibitory effect on growth up to MIC 0.008 mg / ml . The combined hot aqueous extract of PA and FV seeds in a ratio of 1gm to 0.5 gm respectively an 1gm to 1 gm (24and 40.32mg / ml respectively ) also did not have any inhibitory effect of bacterial growth up to MIC 0.002 and 0.004 mg / ml, respectively . The results of this experiment also revealed that hot aqueous extract of similar quantities and concentrations ( 17.28 and 31.74 mg /ml ) of a mixture of several medical herbs like ( PA , FV , Anthemis nobilis , Thymus bovei ,Gum Arabic, Nigella sativa , Althaea , and Cydonia vulgaris ) did not show any inhibitory action on bacterial growth up to MIC of 0.004 and 0.007 mg / ml . It could be concluded that the inhibitory ability of the hot aqueous extract of PA seeds is the best compared to the other extracts used . The inhibition of bacterial growth may need a carefully selected medical herbs in different concentrations and combination .

The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Hussein A Kadhum ◽  
Thualfakar H Hasan2
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Growth ◽  
Broad Spectrum ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
Inhibitory Ability ◽  
Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

scholarly journals Phytochemical Screening and Antibacterial Evaluation of Conventional Antibiotics, Garlic and Ginger on Isolates from Fish Pond Water Samples in Awka, Anambra State, Nigeria

2021 ◽  
pp. 118-132
Author(s):  
O. R. Umeh ◽  
E. I. Chukwura ◽  
E. L. Okoye ◽  
E. M. Ibo ◽  
P. I. Egwuatu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
Water Samples ◽  
Vibrio Parahaemolyticus ◽  
Pond Water ◽  
Inhibitory Effect ◽  
Hot Water ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Conventional Antibiotics

Medicinal plants are used by almost 80% of the world’s population for their basic health care because of their low cost and ease in availability. In the last few decades, many bacteria have continued to show increasing resistance against current antibiotics. Aim: In this study, phytochemical screening and antibacterial effects of conventional antibiotics, garlic and ginger on test isolates from fish pond water samples were evaluated between May-November, 2019. Methods: Standard methods for phytochemical screening and antibacterial analysis were employed. Results: The results showed that amongst the antibiotics used for susceptibility test, Amoxicilin (30 µg) was mostly resisted by all the bacterial isolates except Staphylococcus aureus, Vibrio cholerae, Vibrio parahaemolyticus and Salmonella typhi while erythromycin (10 µg) was unable to inhibit Bacillus subtilis. Ciprofloxacin (10 µg) and pefloxacin (10 µg) inhibited the growth of all the isolates except Pseudomonas aeruginosa. The test isolates showed variable susceptibility to the garlic and ginger extracts (ethanol, methanol and hot water). The extracts inhibited the isolates in descending order; ginger ethanol > ginger methanol > garlic methanol > ginger hot water > garlic ethanol > garlic hot water. Vibrio parahaemolyticus, Vibrio cholerae and Staphylococcus aureus showed little resistant to the extracts while these extracts showed better activity on Klebsiella pneumoniae and Proteus mirabilis. Synergistic effect of garlic and ginger (500mg/ml) inhibited the growth of all the isolates with ethanol extracts having the highest zone diameter (29 mm) on Klebsiella pneumoniae and Proteus mirabilis while hot water extracts had the least zone of inhibition (18 mm) on Acinetobacter calcoaceticus and Vibrio parahaemolyticus. The minimum inhibitory and bactericidal concentration for ethanol, methanol and hot water extracts ranged from 31.25mg/ml to 62.5mg/ml and 62.5mg/ml to 125mg/ml respectively. Conclusion: The outcomes of susceptibility experiment depicted that ethanol and methanol extracts of garlic and ginger (each alone and in combination) showed more inhibitory effect than aqueous extracts and also the combination of ethanol, methanol and aqueous extracts resulted in inhibitory effect greater than each of the extracts when used singly. The use of ginger and garlic for control of fish pathogens appears to be justified.

scholarly journals Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Faramarz Zakavi ◽  
Leila Golpasand Hagh ◽  
Arash Daraeighadikolaei ◽  
Ahmad Farajzadeh Sheikh ◽  
Arsham Daraeighadikolaei ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Aqueous Extract ◽  
Antibacterial Effect ◽  
Juglans Regia ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Oral Bacteria ◽  
Disk Diffusion ◽  
Resistant Bacteria ◽  
Aqueous Extracts

Background. In this study antimicrobial effect of ethanolic and aqueous extracts of Juglans regia bark in Iran was evaluated on four different oral bacteria,Streptococcus mutans,Streptococcus salivarius,Streptococcus sanguis, andStaphylococcus aureus.Methods. Aqueous and ethanol extracts of Juglans regia bark were prepared by using disk diffusion technique and Minimal Inhibitory Concentration (MIC) methods. Tetracycline 30 μg and Erythromycin 15 μg were used as positive control and water as negative control in disk diffusion and MIC methods. Data were analyzed by ANOVA test.Results. The results showed thatS. sanguisandS. mutanswere the most sensitive and the most resistant bacteria against ethanolic and aqueous extracts, respectively. Ethanolic extract had significant antibacterial effect against all tested bacteria. Aqueous extract did not show antibacterial effect onS. mutans, in contrast to ethanolic extract. Aqueous extract had significantly antibacterial effect againstStaphylococcus aureus,S. salivarius, andS. sanguiscompared to control (P<0.0001), but it did not show effect onS. mutanswhen compared with Erythromycin. According to the obtained MIC values, ethanol extract of Juglans regia bark had the lowest rate.Conclusion. The results may provide the basis for using natural antimicrobial substance for oral hygiene prophylaxis purposes.

scholarly journals The Pathogenicity and Transcriptome Analysis of Methicillin-Resistant Staphylococcus aureus in Response to Water Extract of Galla chinensis

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Shizhou Wu ◽  
Yunjie Liu ◽  
Hui Zhang ◽  
Lei Lei
Keyword(s):  
Staphylococcus Aureus ◽  
Carbohydrate Metabolism ◽  
Aqueous Extract ◽  
Transcriptome Analysis ◽  
Biofilm Formation ◽  
Laser Scanning Microscopy ◽  
Aqueous Extracts ◽  
Invasive Ability ◽  
Methicillin Resistant

Aim. Antibiotic abuse contributes to the emergence of methicillin-resistant Staphylococcus aureus (MRSA). It is increasingly important to screen new antimicrobial agents for the management of MRSA infections. G. chinensis, a nontoxic Chinese herbal medicine, is considered a potential antibacterial agent. The aim of this study was to investigate the bactericidal effects of the aqueous extracts of G. chinensis on MRSA. The potential mechanisms of G. chinensis aqueous extract inhibition of the pathogenicity of MRSA in vivo are also discussed. Methods. G. chinensis aqueous extract was prepared and its antimicrobial activities were examined by determining its minimum inhibitory concentration (MIC). Biofilm biomass was determined by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). RNA sequencing (RNA-seq) was used to evaluate differentially expressed functional pathways in MRSA treated with G. chinensis aqueous extract. We validated the role of G. chinensis aqueous extract in the invasive ability and pathogenicity of MRSA in vivo using a rat infectious model. Results. The results indicated that MRSA was sensitive to the G. chinensis aqueous extracts at concentration of 31.25μg/mL. G. chinensis extract led to a reduction in dextran-dependent aggregation and biofilm formation in MRSA. Based on the transcriptome analysis, G. chinensis aqueous extracts significantly downregulated the gene expression related to biofilm formation and carbohydrate metabolism. G. chinensis aqueous extract inhibited the invasive ability and the pathogenicity of MRSA in vivo. Conclusion. The antimicrobial properties of G. chinensis aqueous extract are likely related to its modulation of MRSA biofilm formation and carbohydrate metabolism. G. chinensis aqueous extract is a promising supplementary therapy to lessen or eliminate the use of antibiotics and is a potential tool for the management of MRSA infections.

The relation between inhibitory substances and variability in time to germination of sugar beet clusters

1969 ◽  
Vol 73 (3) ◽  
pp. 337-346 ◽  
Author(s):  
J. P. Battle ◽  
W. J. Whittington
Keyword(s):  
Sugar Beet ◽  
Aqueous Extract ◽  
Inhibitory Effect ◽  
Aqueous Extracts ◽  
Germination Inhibitors ◽  
Positive Correlation ◽  
True Seed ◽  
Germination Characteristics ◽  
Overhead Irrigation ◽  
Good Germination

SummaryEvidence was obtained for the presence of germination inhibitors in the aqueous extract from sugar-beet clusters. A positive correlation was shown between the germination of cress seeds moistened with aqueous extracts from clusters of twenty sugarbeet plants and the germination characteristics of other clusters from the same plants. Inhibition was thus greater in extracts from clusters with poor rather than good germination, and also in extracts from immature clusters or those which had received overhead irrigation. The inhibitory effect was found to be located in the perianth and pericarp tissue rather than in the true seed.

scholarly journals The Effect of Nigella sativa and Piper guineense Aqueous Extracts on the in Vivo Expression Profile of Lipid and Sex Hormones

2019 ◽  
pp. 1-14
Author(s):  
Okoye Ngozi Franca ◽  
Uvbiekpahor Rume
Keyword(s):  
Aqueous Extract ◽  
Nigella Sativa ◽  
Follicle Stimulating Hormone ◽  
Positive Control ◽  
Aqueous Extracts ◽  
Piper Guineense ◽  
Male And Female ◽  
Hormone Levels ◽  
The Third ◽  
Black Seed

Aim: The study investigated the effect of aqueous extract of Piper guineense and Nigella sativa on Testosterone, Estrogen and Follicle stimulating hormone levels in male and female rats induced by diets supplemented with high dose of sucrose and margarine for four (4) week. Materials and Methods: Thirty male and female Wistar rats of average weight (100 g) were used for the study. The rats were arranged into five groups with six rats in each of the groups. The rats had access to their normal feed but sucrose and margarine were used to induce hyperglycemia and hyperlipidemia respectively on the rats with the exception of the rats in the positive control group.  The rats in the negative control were induced using the sucrose and margarine but were not treated using the aqueous extracts. The rats in the Piper guineense group were treated with 2 ml of Piper guineense aqueous leaf extract, while the rats in the Nigella sativa group were treated with 2 ml of Nigella sativa aqueous extract. The rats in the Nigella sativa and Piper guineense group were treated with 2 ml of the combined aqueous extract. Results: The results showed that the extracts had an increasing effect which was time dependent on the hormones. The highest increase was obtained on the third week of feeding when compared to the control. The testosterone levels (mlU/ml) showed for the, positive control (2.28 ± 0.08), uziza leaf (6.45 ± 0.01), black seed (2.85 ± 0.01), black seed &uziza (8.47 ± 0.08)(p<0.05).  The increase for estrogen levels (mlU/ml) showed for the positive control (99 ± 0.82), uziza leaf (98 ± 1.63), black seed (100 ± 1.63), black seed & uziza (113 ± 0.82). Furthermore, the extracts also had an increasing effect on the follicle stimulating hormone levels with the highest increase obtained on the third week (p>0.05).  The FSH levels (mlU/ml)) showed for the positive control (0.23 ± 0.01), uziza leaf 0.25 ± 0.01), black seed (0.54 ± 0.02), black seed & uziza (0.58 ± 0.01)(p<0.05). Histopathological findings also suggest that treatment with the aqueous extracts of P.guineense and N .sativa after inducement salvaged the testes and ovaries when compared to the induced group after the second week of administration. Although the combined mixture N.sativa and P.guineense extract was more effective. Conclusion: The results of the study indicate that the aqueous extract of a combined mixture of Nigella sativa and Piper guineense might be an effective plant in indigenous medicine, which may be used to combat infertility caused by hormonal imbalance.

scholarly journals THE EFFECT OF PSEUDOCEDRELA KOTSCHYI (SCHWEINF.) HARMS EXTRACTS AGAINST STAPHYLOCOCCUS AUREUS GROWTH

2021 ◽  
Vol 4 (1) ◽  
pp. 11-15
Author(s):  
Kokou Atchou ◽  
Koku Amegbo Dakey ◽  
Farida Etroubeka-Tsono ◽  
Amégninou Agban ◽  
Simplice Damintoti Karou ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Ethanolic Extract ◽  
Clinical Strain ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Bactericidal Effects ◽  
Kinetics Of ◽  
Kinetics Of Inhibition

Staphylococcus aureus is a ubiquitous bacteria that causes a serious health problem because of its multi-resistance to antibiotics. The aimed of this study was to evaluate the antibacterial activity of P. kotschyi root against S. aureus ATCC 29213 and S. aureus, a clinical strain. The phytochemical compound was sought in the extracts by standard staining tests and extractions were carried out by fractionation using solvents depletion method with increasing polarity. The method of dilution in liquid medium was used for the antibacterial tests. The results showed that the aqueous extract of the bark was effective against both S. aureus with MICs of 0.39 mg.mL-1. The MICs of ethanolic extract were 0.39 and 0.78 mg.mL-1, respectively on S. aureus ATCC 29213 and S. aureus. The steles of P. kotschyi roots were less effective. The ethanolic and aqueous extracts at concentrations = 2MICs, have been respectively bacteriostatic and bactericidal effects on tested germs. The kinetics of inhibition showed that the aqueous extract of the bark at 0.78 mg.mL-1, completely destroyed the two germs respectively in 4 h and 5 h. The action of ethanolic extract at 0.78 and 1.56 mg.mL-1 occurs late against both S. aureus at 6 h and 7 h. Phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, anthracenes, glycosides, saponosides and sterols, some of that may be responsible for the observed antimicrobial activity. This study proved the antibacterial activity of P. kotschyi roots that can be exploited as an antibiotic in the treatment of S. aureus infections.

scholarly journals Study on the extraction and antibacterial activity of Monascin

2021 ◽  
Vol 251 ◽  
pp. 02061
Author(s):  
Xiaojuan Gao ◽  
Xiaoshi Lu ◽  
Zifeng Wang ◽  
Guangpeng Liu ◽  
Xinjun Li
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Aspergillus Niger ◽  
Inhibitory Effect ◽  
Ethanol Extraction ◽  
Inhibitory Ability ◽  
Strong Inhibitory Effect

Taking monascin as the research object, monascin was extracted from red kojic rice by ethanol extraction and extracted with 60%, 70% and 80% ethanol respectively. Finally, it was concluded that when the concentration of ethanol was 70%, the extraction rate of monascin was the highest, reached 75.68%. The bacteriostatic experiments of monascin extract and monascin fermentation showed that it had strong inhibitory effect on Staphylococcus aureus and Bacillus subtilis, weak inhibitory ability on Escherichia coli and Aspergillus niger, and no obvious inhibitory effect on the growth of Saccharomyces cerevisiae.

Effect of Arak, gall of Quercus infectoriaand Syzgiumaromaticum extracts on the growth and viability ofEntamoeba gingivalis in vitro.

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Sajida S. Hussein Al-Zako ◽  
Ban N. Mohammadali Al-Neamy
Keyword(s):  
Medical Treatment ◽  
Aqueous Extract ◽  
Inhibitory Effect ◽  
Alcoholic Extract ◽  
Aqueous Extracts ◽  
Salvadora Persica ◽  
Oak Trees ◽  
Oak Tree

After the succession of E.gingivalis cultivation on the DMEMmedium. This study was performedin order to identify the effect ofincreasing concentrations from aqueous and alcoholic extracts of a threeplants,known with their usage for medical treatment to dental and oraldiseases,those are Arak sticks (Salvadora persica), gall of oak tree (Quercusinfectoria) and flowering bud of clove (Syzgium aromaticum)on the viabilityof E. gingivalis.Aqueous and alcoholic extracts of Arak sticks showed a clear effectwith the superiority of the aqueous extract in its impact inhibitory effect onE.gingivalis.The results alsoshowed the existence of a negative and inhibitory impact increasingly on the growth ofE. gingivalis with the increasingconcentrations used of aqueous and alcoholic extracts fromgall of oak andflowering buds of clove, with simple superiority of aqueous extracts onalcoholic one for galls of oak trees and alcoholic extract of the of floweringbuds on aqueous one of buds clove.A clear increasing LT50 and LT90 where gained when using aqueousand alcoholic extracts of three plants with an Arak superiority in theelimination of the parasite, specially the aqueous one with the lower time.

scholarly journals Evaluate the efficacy of Antimicrobial activity of aqueous extract of Nigella sativa seeds powder on Staphylococcus aureus

2021 ◽  
Vol 5 (03) ◽  
Author(s):  
Vijitha Paheerathan
Keyword(s):  
Staphylococcus Aureus ◽  
Aqueous Extract ◽  
Skin Diseases ◽  
Fungal Infections ◽  
Nigella Sativa ◽  
Mean Value ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Disk Diffusion Method ◽  
Nigella Sativa Seed

In Siddha medicine system has been classified into Aga marunthu (Internal) and Pura marunthu (External) having 32 forms of medicines in each. The external remedies are very useful for topical ulcers, skin diseases like eczema, psoriasis bacterial and fungal infections. The purpose this study was Evaluate the efficacy of Antibacterial activity of aqueous extract of Nigella sativa seeds powder against Staphylococcus aureus by used disk diffusion method. The result has been evaluated as the diameter of the zone of inhibition of microbial growth showed that the different concentration (100%, 50%, 25%) of Nigella sativa seed powder’s aqueous extract more effective against Staphylococcus aureus. Mean value of the hot extract 100%, 50%, 25% respectively 12.19±1.91mm, 7.63±2.32 mm and 3.78±1.73mm and the mean value of Cold extract were 9.45±1.32mm, 4.21±0.92mm and 2.71±.64mm. Standard maintained 18mm of zone of inhibition .Among the hot and cold extract, 100% of hot extract was showed more significant affect against Staphylococcus aureus which was 12.19mm. Finally this study was concluded that the Nigella seeds powder can be used as an antibacterial agent.      

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